eChapters

Abstract

The objectives of the study is to help to create self-employment and provide proper information for different conventional milk based products. Traditional dairy foods have always played a crucial role in preservation of precious milk nutrients and promotion of its use among masses. The demand for milk and milk products is increasing with increasing of world's population. To meet these needs, high-end technology is needed for emerging the capability of milk production and preserving it by producing high quality milk products. The dairy industry is highly localized owing to perishable nature of milk products. Approximately 50% of the total milk produced in India has been projected to be processed into a variety of conventional milk products, such as khoa, chhena, paneer, dahi, ghee, kalakand and a wide range of sweets in rural areas mostly by the rural citizens. Therefore, setting up a milk-based food production system and updating conventional technologies to manage milk-based products on a commercial scale is very important.

Introduction

Measurement of pH of a solution and preparation of different types/strength of buffers are common practices in a laboratory, particularly in the Biochemistry and Molecular Biology laboratories. Therefore, in this chapter you will be acquainted with the procedures of measuring pH of a solution as well as preparation of some of the commonly used buffers in a laboratory.

Potential or Power of Hydrogen Ion (pH)

Measurement of pH of a solution is measuring the molar concentration of hydrogen ion [H+] in the solution, which is an indication of the acidity or basicity of the solution. The symbol pH stands for “power of hydrogen” and numerical value for pH is just the negative logarithm of the molar concentration of hydrogen ions [H+]. The ionic product of water (Kw ) is the basis for the pH scale. The total hydrogen ion concentration from all the sources and it can be experimentally measured.

The pH scale designates the concentration of H+ (and thus of OH−) in an aqueous solution, which ranges between 1.0 M H+ and 1.0 M OH−. The term pH is defined by the expression

Thus, the ionic product of water makes it possible to calculate the concentration of H+, with given concentration of OH−, and vice versa. The value of 7 for pH of a neutral solution is not an arbitrarily chosen figure; it is derived from the absolute value of the ionic product of water. A solution having pH greater than 7 is basic, and the concentration of OH− is greater than that of H+. A solution having a pH less than 7 is acidic.

Onion growth, bulb initiation, yield, quality and storage life are strongly influenced by temperature, photoperiod, soil, moisture and crop establishment. Different cultivars require specific day lengths and temperature ranges, making regional variety selection and planting schedules essential. This chapter explains the environmental requirements of onion and the consequences of unsuitable conditions, including premature bolting, double bulbs, poor development and disease susceptibility. It examines soil preparation, planting geometry, direct seeding, transplanting and precision establishment. Modern approaches—including sensor-based environmental monitoring, Internet of Things systems, predictive modelling, precision seeding and machine-learning-supported input management—are presented as tools for improving uniformity, resource efficiency and climate adaptability.

Introduction

Different types of culture media are used for culturing microbes and plant tissues. Preparing as well as sterilizing them properly following appropriate method with safety for the desired purpose are important parts of research work. In this chapter, we will discuss briefly about different types of media, their composition, preparation and sterilization in laboratory for microbial or plant tissue culture.

Culture Medium

Ingredients mixed to prepare solid, liquid or semi-solid substance to support the growth of microorganism or plant tissue in vitro (outside the body) is called culture medium. Frequently, it becomes important to grow microorganism or plant/animal tissue in vitro for any or all of the following purposes:

• To study characteristic features or properties of microorganisms.

• To identify the causal organism(s) of infection in a clinical sample, so that appropriate remedy can be determined.

• To culture/multiply microorganism or plant/animal tissues in vitro for research or commercial purposes.

• To prepare/produce biological molecules, vaccines, antigens, proteins, metabolites, etc. in vitro for research or industrial purposes.

SYNOPSIS

Fish has now become an important animal protein for mankind. It is a rich source of essential amino acids, vitamins and minerals. Fish meat is easily digestible compared to other animal meat and has a higher Protein Efficiency Ratio when compared to casein, the milk protein. Fish oils like body oils of fishes like sardine, mackeral, anchoviella, herring, salmon etc. are the richest source for Omega-3 long chain poly unsaturate fatty acid like Eicos- pentanoic acid (EPA) and Docosahexaenoic acid (DHA) having therapeutical applications for man. A number of fishery products and by-products like squalene, chitin, chitosan, oyster meat, mussel meat, shark bones etc.

Introduction

Flowering plants (Angiosperms) are identified by their morphological characteristics like flower, fruit and seeds that help their taxonomic characterization into different species, genera, families and orders. The floral characters which are used for taxonomic characterization of plants include the type of inflorescences, perianth structure, floral symmetry, arrangement of floral leaves, type of androecium, stamens, gynoecium, carpel, ovule, etc. The type of fruit and seed also play important roles in taxonomic classification. In this chapter we will briefly discuss about various morphological features of flowering plants (in botanical terms) that are useful in taxonomy.

Nomenclature of Plant

Nomenclature of plant is the formal and scientific naming of plants. It is related to but distinct from taxonomy for grouping and classifying plants and provide botanical name.

The scientific/botanical nomenclature started with Species Plantarum by Carolus Linnaeus in 1753. Botanical nomenclature is governed by the International Code of Nomenclature for algae, fungi, and plants (ICN), which replaces the International Code of Botanical Nomenclature (ICBN).

ICBN and ICN

International Code of Botanical Nomenclature, (ICBN) was adopted by the VIIth International Botanical Congress held in Stockholm, July1950. While the International Code of Nomenclature for algae, fungi, and plants (ICN) was adopted by the XVIIIth International Botanical Congress held in Melbourne, Australia in July 2011.

Introduction

We come across a variety of chemicals every day in a laboratory. While some of them are relatively safer, others are extremely hazardous. Thus, it is very important to know the potential hazards that can be caused by the chemicals present in the laboratory and the safety measures needed to be followed to avoid the hazards. Additionally, we should also be aware of the steps necessary to be taken on such hazardous situations. According to IUPAC, a hazardous chemical mean the chemical which has the potential to cause damage in various ways. In a laboratory, there are chemicals that might be irritant, carcinogen, respiratory fibrogen, systemic poison, asphyxiant, flammable, etc. which have the potential to cause hazard to the workers or environment. Therefore, it is important to use appropriate procedure to reduce the risk and protect the health/safety of laboratory personnel, the public and the environment.

Types of Hazards Associated with Chemicals

Chemical hazards: The hazards caused by chemicals can be broadly classified into three categories.

1. Health hazard: This is also known as toxic effects of chemical. The risk associated with a chemical in laboratory depends on the extent of exposure and the inherent toxicity of the chemical. Many chemical reactions form the product that might be much more toxic than the reacting chemicals. For example, inadvertent mixing of formaldehyde (a common tissue fixative) and hydrogen chloride results in the generation of bis(chloromethyl) ether, a potent human carcinogen. For most of the chemicals, the route of exposure (through skin, eyes, gastrointestinal tract, or respiratory tract) is more important (Figure 17.1) that must be considered during risk assessment.

Abstract

Modern agriculture largely depends on use of high cost inputs and energy intensive farm machinery. Worlds increasing population and continuously deteriorating soil fertility has alarmed agriculture system to look for some safe alternatives. Organic manures are rich source ofnutrients and humans, therefore crop residue recycling by composting is a best alternate of modern high cost input based agriculture. Compost is an aerobically decomposed organic matter .Bacteria, fungi, actinomycetes algae and protozoa are involved in the process of composting and are found throughout the natural environment. The composting material warms up due to microbial activity and it’s temperature becomes up to 160 ⁰F active stage of microbial activity . There are four types of compositing i.e. Windrow composting, Aerated static pile composting, In-Vessel composting and Vermi-composting. The C: N ratio, aeration, temperature and moisture are major factors affecting composting process. Major advantages of composting are: Aggregate formation, Reduction in bulk density, Increase in hydraulic conductivity, Reduction in loss of soil moisture, Increase in organic carbon content and pH of soil.

Introduction

Proper electrical wiring and earthing are crucial for laboratory safety and functionality. A laboratory houses several installations/equipment that demand stable/continuous power supply. Inappropriate or faulty wiring and earthing may lead to significant risks, including electrical shocks, damage to equipment, and even incidence of fire. Therefore, understanding the requirements of electrical wiring and earthing is essential for designing, construction, and maintenance of laboratory. This chapter aims to provide a basic knowledge of electrical wiring and earthing necessary for a laboratory. Importance of earthing in mitigating electrical hazards and ensuring integrity of electrical installations are also described.

Basics of Electrical Wiring

Basic understanding of electrical wiring is necessary for laboratory safety and efficient electrical functioning. The potential difference between two electrical points (negative and positive) is known as Voltage, which drives the flow of electric current. This is measured as volt (V). The rate of flow of electricity in a circuit is known as Current (I), which is measured as ampere (A). Current can be either direct (DC) or alternating (AC). Opposition/resistance experienced in the flow of current in circuit is known as Resistance (R) and it is measured in ohms (Ω). Higher the resistance means lesser the current flows at a given voltage. The rate at which electrical energy is transferred/utilized in an electric circuit is known as Power (P), which is measured in watt (W). The Power can be calculated as the product of voltage and current (P = V × I).

Structure

Structural unit of edible portion of most fruits and vegetables is the parenchyma cells and makes up bulk of cells. A parenchyma cell is rather thin walled and may be polygonal or cubical in shape.  Walls of parenchyma cells in young plants are composed almost entirely of fibrils of cellulose. In parenchyma cells of plants actively metabolising protoplast portion represents only about 5% of total cell volume. This protoplast is rather film like and is pressed against cell wall by large water filled central vacuole.  Protoplast has inner and outer semi permeable membrane layers between which is confined cytoplasm and its nucleus. 

Introduction

Several hazardous chemicals, microbes, and techniques like recombinant DNA technology are used for research and development work in research Institutes and universities. Therefore, the researchers, technical, helping and supporting staffs as well as the laboratory outputs must be kept free from contaminations. Thus, biosafety is necessary to prevent unintentional exposure/ release of hazardous chemicals, microbes/pathogens, and toxins to the laboratory staff and environment. Following the biosafety guidelines and ethical considerations are essential to maintain safety standards for the researchers as well as the environment. This chapter deals with various biosafety measures and ethical issues to be strictly followed while working in a research laboratory. The required safety level needs to be determined as per the chemical/biological materials are used in the laboratory to keep the researcher/environment safe for sustainable research and developmental activities.

Biosafety

The term biosafety is used to describe the procedure and policies needed to ensure environmental and personal safety. Biosafety refers to the containment principles, technologies and practices that are implemented to prevent unintentional exposure to pathogens, toxins, or their accidental release into the environment. A fundamental objective of any biosafety program is the containment of potentially harmful chemical/biological agents as precautionary safety measures.

Why Biosafety?

With the increasing number of countries adopting molecular biology tools and techniques in life science research and development activities, especially in the areas of agriculture and medicine, the biosafety issues are gaining importance to ensure biological safety for the public and the environment. Conducting research in safe and sustainable manner is not only a personal requirement but essential collective efforts to ensure biological safety for a clean and safe environment. This requires rules, regulations, monitoring bodies, and awareness among the workers as well as the public. Therefore, biosafety per se is an integral part of laboratory research and requires awareness among the researchers so that biological safety can be well taken care from the grassroot level.

13.1    Biochemical Changes During Banana Ripening
    
13.2    Commercial Ripening

Over the past several years, there has been significant change in consumer perception on quality and food safety. Consumers are demanding healthy, nutritious and cost-effective products processed under controlled hygienic conditions. Food safety concern, in recent years, has become dominant and important. The requirement of food safety and quality assurance has never been so demanding as it is now. Globally food safety is being addressed independent of food quality. In anticipation of consumer demands on food safety, food sanitary standards at national and international levels are under constant review. Several inter governmental agencies / organizations are engaged in work related to quality assurance and food safety. Amongst these World Health Organization (WHO) and Codex Alimentarius Commission (CAC) play the major role in protecting public health and defining standards of quality and safety aspects of foods for regional and or world wide usage.

Regulatory Institutions

The quality environment in the Indian food processing sector revolves around compulsory legislation which specify minimum standard and certification system. Legal standards are generally made to have control over the quality of foods offered for sale and to safeguard the consumer from health hazards posed by practice of adulteration. There are basically three types of Indian standards namely

India has a population of one hundred twenty four crore plus and feeding this large a population calls for a strong FPI. The industry also assumes importance in the national economy due to the immense employment generation opportunities it offers and the export revenue it earns. Food Processing Industry (FPI) in India is a sunrise sector that has gained prominence in the recent years. Easy availability of raw materials, changing lifestyles and favourable fiscal policies has given a considerable push to the industry’s growth. FPI serves as a vital link between the agriculture and manufacturing sectors of the economy. Strengthening this link is critical to reduce wastage of agricultural raw materials, improve the value of agricultural produce by increasing shelf-life as well as by fortifying the nutritive value of the food products and ensure remunerative prices to farmers as well as affordable prices to consumers. The industry has very low processing level i.e., 2.2% for fruits and vegetables, around 35% in milk, 21% in meat and 6% in poultry products, which is significantly lower by international standards. For e.g., processing of agriculture produce is around 40% in China, 30% in Thailand, 70% in Brazil, 78% in the Philippines and 80% in Malaysia. Value addition to agriculture produce in India is just 20% with wastage estimated to be valued at around US $13 billion.

India has the second largest producer of food with arable land of 159.65 million hectares and has the highest acreage under irrigation. Next to China, India ranks second largest food producer in the world and has the potential to immerge the biggest with its food and agricultural sector. India accounts for less than 1.5% of international food trade despite being one of the world’s major food producers, which indicates huge potential for both investors and exporters. Food and food products are the biggest consumption category in India

SYNOPSIS

Urban and rural agriculture are different entities in terms of stakeholder involvement and enabling environment for farming but they are dialectically interlinked in providing food and nutrition security of any country. Urban agriculture is more suitable on the production of nutritious crops, while rural agriculture’s first priority is to produce more food from staple. Attaining nutrition security is more complex than it is previously thought. However, while existing systems of production should continue at field levels by developing more nutritionally rich cropping patterns, its integration with farmers’ farming systems remains more important.

Synopsis

Food safety has become increasingly important with globalization due to various factors such as increased volume and diversity of trade in foods, greater public demand for health protection, changing hazards e.g., resistant microbes, more sophisticated methods for detection of hazards, changes in production and consumption pattern, etc. Food safety contributes significantly to food security as unsafe and poor quality food leads to illnesses as well as food wastage. This leads to a strong need to emphasize not only on availability of food but also ensure that the same is safe.

Introduction The method of drying is expressed as applying heat under controlled conditions to remove the water or moisture from foods to get a dry product with low moisture content. The primary commitment of drying is to extend the shelf-life of foods by diminishing their water activity. Due to low water activity, microorganisms which cause food spoilage and decay and many of the enzymes which promote undesired changes in the chemical composition of the food are unable to grow, multiply or function. The need for drying in the food industry is increasing day by day, and Novel dehydration techniques are being invented for a more efficient drying process, less energy-consuming, and preserve the product characteristics and nutritional profile. Further, the drying technology is critical for moisture removal from food and non-food materials. Small scale farmers use this food preservation method as an indigenous method and the food processing industry as modern technology. Dried products have numerous benefits compared to raw products, including an extended shelf-life and reduced packaging, storage, handling, and transportation costs. However, drying is a highly energy-intensive food processing operation.

4.0. Introduction

Quality is a measure of the degree of excellence or degree of acceptability by the consumer. By quality, one can differentiate the individual unit of a product from the other units and can determined the degree of acceptability of the individual unit by the consumer. The quality may also be defined in term of end use and may vary depending upon consumer’s perception and need. According to USDA Marketing Workshop Report (1951) “quality is the combination of attributes or characteristics of a product that have significance in determining the degree of acceptability of the product to the user”. For industry, quality is a measure of purity, strength, flavour, colour, size, maturity, workmanship and conditions or any other distinctive attributes of the product.

According to Code of Federal Regulations “Quality is the inherent properties of any processed product which determine the relative degree of excellence of such product and includes the effect of preparation and processing and may or may not include the effects of packaging or added ingredients/ additives”.

In India, people are mostly vegetarian, depending largely on cereals and pulses, as their staple foods, which provide the main source of dietary proteins and calories. The proteins from pulses are known to be inferior quality, due to the deficiency of sulphur containing amino acids as well as due to other factors like digestibility, availability of amino acids, anti-nutritional factors, etc. The anti-nutritional factors present in this type of food, including their contents of enzyme inhibitors, lectins, flatulence factors, tannins, phytic acid and saponins. Anti-nutritional factors can cause detrimental effects to humans and animal growth and performance by impairing intake, uptake, or utilization of other food and feed components, or by causing discomfort and stress to humans and animals. The anti-nutritional factors mainly occur in pulses and grain legumes and foods and feed material prepared from grain legumes and pulses.

Substance which, from nutritional point of view, interferes with normal growth, reproduction or health when consumed regularly in amount existing in a normal component of diet should be considered as harmful and toxic. A significant part of human population relies on legumes as staple food for subsistence, particularly in combination with cereals. They are unique foods because their rich nutrient content including starch, protein, dietary fibre, oligosaccharides, phytochemicals (especially the isoflavones in soybean) and minerals. Their nutritional contents contribute to many health benefits to human.

Advancements on sugar processing

India has popped out as one of the largest sugar processing country throughout the world. Through the collaboration of research and development carried out in the field of sugar processing, this industry has witnessed the vast changes in term of technology. Important considerations such as productivity improvement and cost reduction were the main objectives in the field of sugar processing. Sulphur dioxide was earlier used but afterwards lime treatment in clarified juice helped in the processing of white sugar from sugar cane. Since then the continuous progress and improvement has been made and advanced techniques are introduced for manufacturing of sugar with modern tools.

Ramjeawon (2004) reported that farmers and agriculture workers grow sugar cane and beet root for sugar production,providing about 20 kg per capita and that is equivalent to almost 13 percent energy requirement. Fechter et al. (2001) reported that production of the white sugar was focused more on juices rather than molasses which were taken as the raw material. Various processes such as ultra-filtration, de-mineralization and de-colorization were used for the production of white sugar. The traditional refining process wascheaper, and effective in terms of purification and juice purification was the method used for the processing of white sugar directly from the sugar cane. Crystallization was one of the process which is used for increasing the recovery of sucrose and it reduces the molasses production. Chromatography was used in the sugar industry for the separation of sucrose. Another method was ion exchange demineralization which was used in the sugar industry for the removal of minerals from the liquid sugar. Later adsorption has been incorporated to be used de-odorization and de-pigmentation. Membrane filtration is latest technique used in the process which was used for maintaining the quality of white sugar by removing unwanted particle from the juice. Velásquez et al.,(2018) reported that thermal processes are also used for the production of sugar from sugar cane. Non centrifugal cane sugar processing is used for this purpose and the product is obtained by evaporating the sugarcane which has the unique flavor and aroma. Later the product is used as the sweetener and has a traditional characteristics flavor and optimum nutrition.