eChapters

INTRODUCTION

Mizoram is a hilly terrain lying in the north eastern part of the country. The total geographical area of the state is 21, 087 sq. km and lies between 92º 15/ to 93º 29/ E longitude and 21º 58/ to 24º 35/ N latitude. Out of six agro climatic zones of the NEH region, Mizoram comes under sub tropical hill zone and posses a sub tropical and humid climate due to high rainfall (Fig. 1). These climatic conditions are very conducive for the cultivation of a large number of agri-horticultural crops. The physiography of the state is divided into hills and valley lands. About 90 % area of the state comes under hills and only 10 % area is under valley. The valley lands are sporadically distributed in small patches except in Champhai district. The slope of the hills in Mizoram is much steep as compared to the other states in the North Eastern region. The land use statistics of the state is presented in Table1.

Biofilms are resistant to antibiotics even at high concentrations (Costerton et al., 1999).This could happen when the diffusion of the antibiotic within the biofilm matrix is inefficient and results in slow growth rate of biofilm cells (Davey et al., 2000), there could be the presence of persistent cells and antibiotic-resistant small-colonies (Hogan and Kolter, 2002; Kolenbrander, 2000).Studies have proved that antibiotics can inhibit biofilm formation. An antibiotic azithromycin, is known to inhibit Pseudomonas aeruginosa biofilm. Biofilm colonies become resistant at higher concentrations of the antibiotics whereas at low concentrations these can be induced (Mah and OToole, 2001). P.aeruginosa is commonly present in the lungs of patients infested with cystic fibrosis. The clinical isolates show resistance to azithromycin (MIC>64 pg/ ml).However, low doses of azithromycin have shown improvement in lung function in patients with cystic fibrosis (Borriello et al., 2004). If antibiotics are given at low concentrations (below the MIC) than it induces biofilm formation in a variety of bacterial species. Sub-MIC concentrations of tobramycin can induce biofilm formation in P. aeruginosa. The use of antibiotics in agriculture, as growth promoters have shown to expose bacteria to low levels of the drugs and has caused havoc (Smith et al., 2002). Rachid et al. 

Jay et al., (1995) and Joerger et al., (2003) have written the role of bacteriocins as an alternate to antibiotics. Bacteria produce bacteriocins which are proteinaceous in nature or peptidic toxins. These inhibit the growth of similar or closely related bacterial strain(s). There are different types of bacteriocins which are classified based on the producing strain, common resistance mechanisms and mechanism of killing. Ahmad et al., (2013) have isolated antifungal compound for food preservation. Martin et al., (2018) and Batish et al., (1997) found antibacterial compounds from fungus. Mahboubi et al., (2019) studied the potential of Zataria in IBS. There are bacteriocins from gram-positive bacteria known as the colicins, the microcins and the bacteriocins from Archaea (Cascales et al., 2007). The bacteriocins from E. coli are called colicins (formerly called ‘colicines,’ meaning ‘coli killers’). These have been studied widely. Earlier called colicin V and is now known as microcin V. It is very small and is produced and secreted in a different manner than the classic colicins. Adrienne et al., (2016) have studied the colicin resistance.

Bio-stimulants are increasingly used to improve crop performance while reducing dependence on intensive chemical inputs. They include amino acids, protein hydrolysates, humic substances, seaweed extracts, beneficial microorganisms and plant- or microbial-derived compounds. These substances can influence root architecture, nutrient assimilation, hormonal balance, photosynthesis, stress tolerance and disease resistance. This chapter examines the effects of different bio-stimulants on onion seed germination, vegetative growth, bulb development, yield, quality and storage life. It discusses their modes of action, application methods and integration with nutrient-management programmes. Bio-stimulants are presented as promising components of environmentally responsible and resource-efficient onion production, while areas requiring further research are identified.

Abstract

The applications of enzymes are awe inspiring marvels of nature. Scientists can use enzymes to explore all aspects of biology; from molecules to cells to tissues to organs to organism as a whole. For the purpose of food and drinks, enzymes have been exploited by men for thousands of years without really knowing what they are and how do they work? Enzymes have played a pivotal role in many aspects of life since the dawn of life. Today, biocatalysis is being used as a useful alternative to chemical process technology. The fact that enzymes work under much milder conditions oftemperature, pressure and acidity, thus, obviate harsh processing conditions and thereby decrease the possibility of damage to heat labile substrates and save energy. Enzyme technology being eco-friendly can be justifiably termed as‘Green technology’. Due to their high specificity and high catalytic rate, enzymes prevent wastage and reduce time of processing / manufacturing, respectively. The ability of enzymes to act on wide variety of substrates has made them useful tools to exploit cheaper starting material in food industry. Reportedly, over 45% of the enzymes produced are being used in food industry and remaining is shared by detergent (34.4%), textile (11%), leather (2.8%) paper and pulp (1.2%) and other industries (5.6%). Enzymes serve a myriad of functions in food processing, from maximizing juice extraction of pectin-rich fruits to extending shelf-life in baked goods through slowing starch retro-gradation. Indian food processing industry has seen significant growth and changes over the past few years. Though, the market of enzymes is increasing at a fast pace but most of the enzymes of the food sector are coming from overseas. Seeing the unparallel role of enzymes in food processing sector as in multifaceted domains, the researchers should emphasize upon finding novel enzymes and explore their untapped commercial benefits using modern techniques of enzyme engineering, immobilization and biotechnological interventions.

Abstract

Meat is considered to be a highly perishable food commodity because of its biological composition. Thus, to extend its shelf life and maintain its nutritive value, there is an urgent need to find out alternatives for synthetic anti-microbial and anti-oxidants. This will not only serve the purpose of meat product manufacturer’s but will also satisfy the consumers’ demand for minimally processed meat products devoid of synthetic preservatives. This review discusses the natural antimicrobial and anti- oxidants sources viz., various essential oils, their composition and application in different meat and meat products. Essential oils from basil, rosemary, thyme, oregano, ginger, basilica, balm, coriander, rosemary and clove have exhibited a better potential to be used as an antimicrobial agent in meat and meat products. They owe their anti-microbial properties to the phenolic compounds present in them. They dislocate cell membrane and disrupt functionality of bacterial cell membrane and eventually leading to the release of intracellular components. However, essential oils are not widely used due to the pungent and intense aroma imparted to the food, thus there is still need to work out on the novel technologies which can not only increase food shelf life but at the same time, are sensorially acceptable to the consumers.

Introduction

Fermentation has been an integral part of food processing throughout the history of mankind. In beginning, it was mainly preservation method, then it became key process in substantial sensory characteristic development and now it is utilized to improve overall properties of outcome from major industries like bakery, dairy, beverages, etc. The human understanding of controlled fermentation process changes its fundamental objective by exploring its success in development of rheological and texture characteristics.

The biochemical transformation of raw food due to fermentation affects the organoleptic as well as rheological properties of a product. The changes in rheological as well as sensory properties is related with the modification of major component by the action of microorganism. For example, transformation of polysaccharides in bread dough by action of yeast produce ethanol, CO₂ and other simple compound which modify sensory as well as rheology of dough. The major micro-structural, bio-compositional and bio-chemical changes are due to microbial action are frequently described to understand the effect of fermentation on rheological as well as sensory properties of food products. The rate and quality of these associated major changes mainly depends upon concentration of saccharides for growth of microorganism as well as nature and existing microflorae. Hence, a proper monitoring of changes in rheological and sensory properties is necessary to optimize the controlled fermentation as well as design of process parameters from engineering and consumer point of view.

The biochemical changes directly responsible for changes in basic rheological and sensory characteristic including flow behavior, consistency, viscoelastic properties, hardness, adhesiveness, color, aroma, flavor and mouthfeel. The food industry is so diverse and exploring new and possibilities to catch diverse consumer worldwide, a proper understanding of effect of fermentation on mentioned properties will be key to hold competitive market. Hence, the present chapter will cover the basics of rheology and its role in food industry, effect of fermentation on rheological and sensory attributes of major class of food.

Abstract

Dietary fibers mostly include non-starch polysaccharides such as celluloses, hemicelluloses, gums and pectins, lignin, resistant dextrins and resistant starches. Based on their water solubility, dietary fibers may be divided into two forms insoluble dietary fiber which includes celluloses, some hemicelluloses and lignins which is fermented to a limited extent in the colon and soluble dietary fiber which includes f-glucans, pectins, gums, mucilages and hemicelluloses that are fermented in the colon. Resistant starch generally considered to be represented by the total amount ofstarch and the product of starch degradation that are not digested in the small intestine andpass into large intestine, and regarded as a component ofdietary fiber. Herbal nutraceutical is used as a powerful instrument in maintaining health and to act against nutritionally induced acute and chronic diseases, thereby promoting optimal health, and quality of life. Dietary spices in their minute quantities has an immense influence on the human health by their antioxidative, chemopreventive, antimutagenic, anti-inflammatory, immune modulatory effects on cells and a wide range of beneficial effects on human health. Nutraceutical is the hybrid of ‘nutrition’ and ‘pharmaceutical’. Nutraceuticals, in broad, are food or part of food playing a significant role in modifying and maintaining normal physiological function that maintains healthy human beings. Functional foods are including whole foods and fortified, enriched, or enhanced foods or dietary components that may reduce the risk of chronic disease and provide a health and physiological benefit beyond the traditional nutrients it contains.

SYNOPSIS

Fish has now become an important animal protein for mankind. It is a rich source of essential amino acids, vitamins and minerals. Fish meat is easily digestible compared to other animal meat and has a higher Protein Efficiency Ratio when compared to casein, the milk protein. Fish oils like body oils of fishes like sardine, mackeral, anchoviella, herring, salmon etc. are the richest source for Omega-3 long chain poly unsaturate fatty acid like Eicos- pentanoic acid (EPA) and Docosahexaenoic acid (DHA) having therapeutical applications for man. A number of fishery products and by-products like squalene, chitin, chitosan, oyster meat, mussel meat, shark bones etc.

Abstract
The United Nations predicts that by 2050, the global population will reach 9.6 billion, leading to urgent challenges such as ensuring food security and reducing greenhouse gas emissions. Climate change, coupled with biotic and abiotic pressures, will significantly impact global food production. Despite advances in agriculture, malnutrition remains a widespread issue, affecting millions, especially in developing countries. Protein-energy malnutrition, which affects over 170 million children and women, poses a significant threat to national economic development, increasing healthcare costs and reducing productivity. One key solution to combating malnutrition lies in promoting legumes (pulses), which are nutrient-dense, protein-rich, and vital for both human and animal consumption. Pulses have been a staple in diets
for centuries, providing essential nutrients like protein, fiber, vitamins, and minerals. They are not only beneficial for human health, reducing the risk of heart disease, diabetes, and cancer, but they also contribute to sustainable agriculture. Legumes can fix atmospheric nitrogen, reducing the need for synthetic fertilizers, improving soil health, and increasing agricultural productivity. However, despite their benefits, pulse production lags behind other staple crops like cereals. With proper management, legumes could play a vital role in alleviating global hunger, particularly in developing countries. Increased awareness and efforts to incorporate legumes into farming systems and daily diets could help meet the growing global demand for nutritious, sustainable food.
Keywords: Climate change, Food security, Global hunger, Legumes, Proteinenergy malnutrition, Sustainable agriculture

Millions of people worldwide suffer from food insecurity and malnutrition, which are exacerbated by issues including pollution, overcrowding, climate change, and economic hardship. These problems highlight the necessity of changing food systems for better immunity and health since they lead to both undernutrition and overnutrition. Essential carbs, fiber, fats, and important micronutrients including iron, calcium, potassium, zinc, and magnesium are all found in nutrient-dense grains such as foxtail, kodo, proso, tiny, and pearl millets. These millets are widely accessible and have several health advantages. For example, pearl millet is high in amylose starch and contains essential minerals like phosphorus and magnesium, whereas proso millet is a good source of iron, calcium, potassium, and B-vitamins. B vitamins, iron, calcium, and zinc are all found in kodo millet, while finger millet is a great provider of calcium, iron and magnesium. The nutritional profile of millets is further improved by processing methods like milling, germination, extrusion, and fermentation. Prominent research organizations, including as ICRISAT, ICAR, and CGIAR, are actively working to conserve millet and create better types to combat chronic illnesses and malnutrition. Targeted improvements in disease resistance and nutrient content are now possible because to biotechnology advancements. For improved worldwide health outcomes, dietary patterns must nonetheless be changed and these nutrient rich grains incorporated into contemporary food systems, notwithstanding current advancements.

Abstract

Nanotechnology deals with understanding and control of matter at 1-100 nm dimensions. Nano-sized materials have been part of everyday life in the form of biological entities and processes. Many of the structural building elements in foods are colloids in nature and are built up as a result of self assembly of nano-sized molecules in particles or at interfaces. The most important food raw materials-proteins, starches and fats undergo structural changes at the nanometer and micrometer scales during normal food processing. Unique phenomena occurring at this length scale are being exploited for novel food applications. Functional foods are products, in food or drink form, which influence specific functions in the body and thereby offer benefits for health, well-being, or performance beyond their regular nutritional value. Nanotechnology promises to provide means of altering and manipulating food products to efficiently deliver nutrients like proteins and antioxidants for precisely targeted nutritional and health benefits. Nanotechnology applications for functional food ingredients and additives offer range of benefits to the consumer in terms of innovative, tasteful and healthy food products. Nanoencapsulation systems aid smart protection and delivery of bioactives so as to ensure their stability, taste, colour, safety and bioavailability. The advent of nanotechnology has opened up innovations in food packaging with improved stability and barrier properties. However, need exists to develop regulatory systems for managing any risks associated with functional nanofoods and the use of nanotechnologies in the food industry.

In this century the usage of plastics had become very common. However, it is important to know as to what kinds of plastics are used for food handling and storage and the health hazards caused by using it. It has been observed that plastic packaging plays a significant role for the enhanced shelf life and in the ease of storage and cooking for many foods. Some kinds of plastic materials which are widely used for handling and storage of food and water include polyethylene terephthalate (PET). It is used in soft drinks, water, sport drinks, ketchups, salad dressing bottles, peanut butter, pickle, jelly and jam jars. This plastic is strong, heat resistant, resistant to gases and acidic foods. It can be transparent or opaque. It does not leach any chemicals that are suspected of causing cancer or disrupting hormones and can also be recycled.

Usage of High density polyethylene (HDPE) has been found to be common in milk, water, juice bottles, yogurt, margarine tubs, grocery, trash and retail bags. High-density polyethylene is stiff and strong but is not heat stable (it melts at a low temperature). It does not leach any chemicals that have a role in causing cancer or disrupting the hormones. HDPE can be recycled. Low-density polyethylene (LDPE) is used into making films of various types, some bread and frozen food bags and also in the making of squeezable bottles. Low-density polyethylene is relatively transparent. Many of the films are not heat stable either and may melt the food in case it is touching it. However, polypropylene (PP) is more heat resistant, harder, denser and more transparent than polyethylene and is therefore used for heat-resistant microwavable packaging and sauce or salad dressing bottles. Polycarbonate is clear and heat resistant. It is also durable and often used to make refillable water bottles and sterilisable baby bottles, microwave ovenware, eating utensils, plastic coating for metal cans. Tiny amounts of bisphenol A are formed when polycarbonate bottles are washed with harsh detergents or bleach (sodium hypochlorite). At high levels of exposure, bisphenol A is potentially hazardous as it mimics the female hormone estrogen. In addition, polystyrene (PS) and polyvinyl chloride (PVC) are also used during food material transportation and handling in supermarkets. Modern food safe plastic bags are plasticizer-free which do not leach harmful chemicals into the food during cooking.

Probiotics are measured in colony forming units (cfu). CFUs are generally measured in the millions or billions per serving. Probiotics are most commonly beneficial bacteria, but can also be friendly fungal or other organisms, that are typically freeze dried to stabilize them in an inert state during storage and production. Then their continued stability and viability, as measured by CFU counts when cultured, is dependent on limiting their exposure to stimulating environmental conditions such as temperature and moisture. Besides refrigeration, this protection can be done by packaging in glass or by adding freshness packets that helps to absorb and reduce moisture in the package.

Introduction

Since the last century, nutritional science has made a remarkable contribution to the biological sciences through identification of diverse nutrients and their sources, functions, requirement, deficiency symptoms and their remedial measures etc. The advent of high-throughput sequencing technologies together with bioinformatic tools identified the presence of 100 trillion of microorganism belonging to all forms of life in the gastrointestinal tract. In fact, the whole gut microbial consortia are considered as a virtual organ in human and animals and is responsible for the development and functioning of series of essential physiological activities including digestion, absorption, metabolism, immunity, homeostasis, brain and gut development, resistance against disease etc. According to the modern scientific dogma, the human being is in fact a “Superorganism”, carrying consortium of numerous symbiotic Eukarya, Bacteria, Archaea and Viruses. The gut microflora has been co-evolved with human and presumed to maintain an intricate balance among different microbial communities. Upon introspection about the functionality of the gut microflora, it is amply clear that a few of these microorganisms have the ability to make healthy state; while other members are responsible for health-related problems including food poisoning, diarrhoea, abdominal pain, indigestion, inflammatory bowel disease, predisposition to colon cancer, compromising immunity, allergy etc.

Introduction

Food is any substance or a product obtained from nature, which contains nutritive and non-nutritive components that help to maintain or improve the health of an individual if consumed in optimal amount (Sukhneet and Malhotra, 2014). Globalization of food production and procurement makes food chains longer and more complex and increases the risk of food safety incidents. Infections and diseases arising from contaminated/adulterated food remain threats to global public health (Havelaar et al., 2015; Luo et al., 2019). The WHO reports shows that every year foodborne diseases cause the death of 33 million people including many children (WHO, 2019). Food safety is linked to the presence of foodborne hazards in food at the point of consumption. Since food safety hazards can occur at any stage in the food chain it is essential that adequate control be in place. Therefore, a combined effort of all parties in the food chain is required to deliver safe food products to the final consumer. Effective and harmonized food safety systems shall manage and ensure the safety and suitability of food in each link of the supply chain.

Although the food industry has the prime responsibility of ensuring safe foods in most societies, the maintenance of a safe food supply is regarded as a responsibility of the government of that country. This responsibility has, over many years, led governments to adopt legislation, standards and regulations for food processing and to impose penalties when a business fails to meet the expectation. As with any legislation, an effective system for detecting failures to comply with the legal requirements increases compliance and hence, in this case, promotes food safety. Governments have an obligation to protect consumer health and this includes protection against food borne diseases. Food standards are therefore necessary to aid governments in protecting public health. However, the standards can only be effective when they are enforced through an efficient food control system. This therefore requires comprehensive legislation inspection, food monitoring and food borne surveillance. An efficient food control system must be supported by competent staff with good laboratory services. Political and institutional support and stability are also important elements.

Abstract

In the present horticultural scenario of India, this is the burning issue as how best the underutilized horticultural crops can be exploited. There is tremendous potential for these crops especially for diversification. These plants can generate employment for millions especially to the natives ofdeveloping counties besides nutritional security. Traditional wisdom of Indian farmers if used properly can pave the path for transforming the economy of developing nations. There are great opportunities for the people to identify crops, explore their market, standardize their cultivation and processing techniques, document them and provide jobs to millions. This will salvage the people from exploitation by multinational companies of developed nations and can give a new direction to sustainable agriculture.

Due to the globalization of the food supply there has been a need to strengthen food safety systems in and between the countries. In this regard the World Health Organization (WHO) has promoted efforts in improving the food safety from farm to plate which started on World Health Day, 7 April 2015. The World Health Day 2015 slogan was “From farm to plate, make food safe”. The informal food production and marketing system is still strong in most countries of the South-East Asia Region, for the enforcement of food safety regulations. Traditionally, the preferences have been for hot and well-cooked food. These habits are partially responsible for preventing foodborne infections. In many countries street food has become very popular as it is cheap. Hygienic conditions are improving, provided that potable water supply and clean facilities are ensured by municipal authorities. Many waterborne and diarrhoeal diseases could be prevented with the introduction of bottled drinking water. The awareness and education on food safety by the consumers has strengthened in the enforcement of food standards. This has improved the hygienic practices and has helped in the prevention of foodborne illnesses. The “WHO Five keys to safer food” serves as the basis for educational programmes to train food handlers and educate the consumers. These help in preventing the foodborne illnesses. The important points are to keep food surfaces clean, washing of all utensils, plates and cutlery, keeping the raw food separate from the cooked food. Cooking food thoroughly to the appropriate temperature, keeping food at safe temperatures, both for serving and storage and using safe water and raw materials.

Carrot, radish, turnip and beet root are major root crops grown in India. In addition, root crops like rutabaga, parsnip, parsley, chervil and celeriac are grown in a limited scale in different parts of the world. There are several other roots and modified underground plant parts that grow in forests and consumed by tribes, but they are not commercially cultivated. Carrot, radish, turnip and beet root are generally consumed as cooked vegetables or fresh salads or as pickled vegetables.

5.1. Carrot

Carrots belong to the Umbelliferae family, named after the umbrella-like f lower clusters that plants in this family produce. As such, carrots are related to parsnips, fennel, parsley, anise, caraway, cumin and dill. Carrot, Daucus carota L. is believed have been primarily originated in Afghanistan, South western Asia and Mediterranean region. Daucus carota ssp. Carota is the most common wild form in Europe and South West Asia, and the present day cultivated carrots have most probably originated from this sub-species.

Haryana, Andhra Pradesh, Punjab and Tamil Nadu, Karnataka, Uttar Pradesh and Assam are the major producers of carrot in India.

Cultivars/Varieties: Varieties with long, orange coloured and smooth roots are preferred in India. Both indigenous and exotic varieties having different root length, shape and colour are grown. Temperate or European type varieties are Nantes, Half long, Early Nantes, Chantenay, Chaman, Pusa Yamadagni and Ooty-1, which require low temperature of 4-8°C for flowering. Tropical or Asiatic type varieties do not require low temperature for flowering and can be grown in plains also. The varieties belonging to this group are Pusa Kesar, Pusa Meghali and Hisar Gairic. Some varieties like Gold King, Indian Kuroda and Super Kuroda are also marketed by private seed companies.

INTRODUCTION

Rubber cultivation in regions extending from 10°N to 10°S latitude is known as the traditional region and the areas other than its natural climatic origin is termed as the non-traditional region. Northeast India comprises majority of the non-traditional areas in India with major climatic constraints like high seasonal rainfall, excessive precipitation and to some extent dry conditions. These areas extending from 22 to 29^°N latitude, holds promise in extending rubber cultivation because of its exceptional climatic make-up and abundant amount of annual precipitation (2000 mm) distributed over a fair period of time.

This perennial crop generally yields latex rubber at the 7th year after planting (with an economic life span of 20 years), although the period (immaturity period) it requires, is higher in the non-traditional region (Rubber Growers Companion, 2006). This immaturity period has also been shown to reduce with water availability in sub-humid climates (Vijayakumar et al. , 1998). Harvest of this crop is by tapping. Tapping is a process of controlled wounding during which thin shavings of bark are removed, thus opening the latex vessels which exude latex. Dry rubber yield is obtained after the latex is coagulated and rolled into sheets (about 500gm) which is then smoked and dried.