eChapters

Concept: It’s a natural process that modifies the cytology, physiology, biochemistry, and physical characteristics of seeds. These modifications lower viability and ultimately result in the seed’s death. A decline in the proportion of germination indicates degradation, and weak seedlings are produced by seeds that do germinate. The factors that impact the ability of seed to be stored include the seed’s quality at the time of storage, its pre-storage history (environmental factors during pre- and post-harvest stages), its moisture content or ambient relative humidity, the temperature of the storage environment, the amount of time the seed is stored, and biotic agents. Seed will inevitably get damaged when being stored (Balesevic-Tubic et al., 2005). The quality of the seed at the time of storage, its pre-storage history (environmental factors during pre and post-harvest stages), its moisture content or ambient relative humidity, the temperature of the storage environment, the length of time the seed is stored, and biotic agents are factors that affect the seed’s ability to be stored. Different physical changes can happen to seed during storage because of factors like temperature, moisture content, and length of storage. The viability and vigor of the seeds may be impacted by these modifications.

Concept: Reduction of seed moisture content to the acceptable levels for enhancement of storability and longevity is known as seed drying.

The moisture content of seed is negatively related to its storability. As the moisture content of seed increases, its storability decreases. Adequate drying of seed prolongs viability for reasonably long periods without cold storage. The process of drying begins with physiological maturity till storage/packaging of seeds to avoid deterioration. The seed is hygroscopic in nature therefore it adjusts its moisture content in equilibrium to the existing environment.

Seed Drying

Seed drying is the process in which an environment with low humidity is created to remove moisture from the seed up to the desired level without any damage to its viability and physical appearance. This seed is stored at low moisture content under the low relative humidity to enhance its longevity. The initiation of seed moisture content and the desired level are what dictate the amount of the drying process, and these elements are determined by the following

• Type of seed: This will consider the longevity behaviour, seed content, and seed structure (i.e., how well the species stores energy).

Concept of Delinting

Cotton seed clumps because of linters getting trapped on them after ginning. Gin-run seeds have very low flowability and do not singulate, making cleaning, upgrading, and precise metering in planting operations challenging or impossible. To singulate the seed and increase flowability, a variety of techniques have been and are employed. Most of the techniques entail removing the tags and linters entirely or partially, although other coating techniques have also been attempted with mixed results, both financially and technically (Mezynski, 1966; Webber and Boykin, 1907).

Methods of Delinting

1. Mechanical Delinting

Mechanical delinting is the usual technique used to improve the flowability of cotton seed. As previously indicated, mechanical delinting basically involves resharpening with finer, closer-spaced saws to remove part of the commercially valuable linters.

During processing seeds are cleaned, dried, and graded which are then treated with specific fungicide and insecticide. Seed at this stage is at best quality, which are then packaged into various packaging materials in a specific weight. Packaging or bagging of processed and treated seed is a requisite for easy handling of the bulk seed. Seed packaging can be defined as filling with seed, weighing and sealing the container whereas seed package as storage container where the seeds are kept for marketing or shipping or till the next sowing time.

The Packaging of Seed Involves

1. Filling bags/ containers of uniform size with seeds of known weight.

2. Providing leaflets inside the package with information about any specific cultural practices needed.

3. Attaching labels or certification tags having information regarding all the quality parameters.

4. Temper-proof sealing of the bag.

5. Storage or transportation of the bags.

Objective

Vegetable seed separation requires specialized training. A little carelessness during the seed extraction process can seriously harm the plant’s viability and vigor in addition to its outward look. Inadequate extraction methods can also result in in-situ germination. The seed can be separated by the following methods.

1. Manual Method

(a) Maceration e.g., watermelon,

(b) Crushing e.g., brinjal,

(c) Scraping e.g., cucumber

(d) Separated e.g., muskmelon,

(e) Scooping e.g., pumpkins and

(f) Extraction e.g., squashes.

Concept: Seed blending is the process of merging two or more types of the same species, for as Kentucky bluegrass cultivars Merion, Baron, and Park. Contrarily, seed mixing refers to the blending of two or more species, however every species may have more than one variation represented, such as Jamestown and Cascade chewing fescue combined with Merion and Fylking Kentucky bluegrass. Seed is typically distributed in blends, primarily Kentucky bluegrass blends, for sod growing purposes. For home lawns, parks, and other utility areas where upkeep levels would not be adequate to maintain pure Kentucky bluegrass turf, mixes of different grasses are typically employed. Naturally, when it comes to pure seed quality, no blend of grass seeds is superior to the individual ingredients. Every time a new blend needs to be made; extreme caution must be taken to ensure that the mixing equipment can be completely cleaned to prevent any contamination from unwanted species—bent grasses are perhaps the most challenging to deal with.

Pre-Treatment Before Sowing

The natural conditions necessary for seeds to germinate are increased by pre sowing seed treatment. Many plants and trees’ seeds require a specific amount of time to mature in their immature portions and to overcome dormancy, which is the absence of certain elements that impede germination, so that the seeds can sprout. Depending on the type of seed, different environmental factors promote different levels of germination. For example, some seeds need to be kept in a very damp, cold environment, while others need to be kept in a dry, hot environment. Different techniques are employed to support seed survival and speed up germination, depending on the size of the lot, the species, the local conditions, and the availability of pertinent tools and facilities. Many species’ seeds don’t germinate successfully unless they are exposed to specific circumstances. Dormancy is the state in which seeds do not germinate unless certain circumstances are met. Conditions in the natural world could include being near fire or being eaten by animals.

Concept: A few of the numerous environmental elements that affect seed quality maintenance are moisture, temperature, humidity, and storage conditions. When some seedborne diseases or pests like insects are taken into consideration, seed quality can still be negatively impacted. Applying one or more pesticides to seed is the most economical and efficient way to keep pests away and improve seed quality.

Because pesticides are harmful, handling treated seeds after application requires additional caution and safety measures.

Definition of Treated Seed

The definition of “treated” is “application of a pesticide or subject seed to a process intended to reduce, control, or repel insects, disease organisms, or other pests which attack the seed or seedlings.”

Stored grain pests are one of the major constraints in obtaining quality seeds. They cause substantial yield loss in storage conditions affecting both the quality and quantity of the produce. Apart from causing direct loss to the grains, they also make them unfit for consumption through their excreta, scales and wings resulting in development of unpleasant odour (Upadhyay and Ahmad, 2011). They also act as a source of medium for growth of fungi or bacteria (Daglish et al., 2018). The method of infestation of stored gain pests is different from the insect pests infesting crops under field condition. Generally, the infestation of storage pests starts after harvesting. It has been reported that in India, there is a loss of about 14 million tons worth Rs. 7000 crores due to the infestation by different storage pests (Chaubey, 2022). The post-harvest loss in terms of quantity and quality by different storage insect-pests accounts for about 10-30 per cent (Rajasri and Kavitha, 2015; Yaseen et al., 2019).

Sanitation

Sanitation and maintaining good hygiene conditions are the important aspect of seed storage in storage godowns. Maintenance of good hygiene conditions will maintain the quality of produce in the long run (Mahapatra et al., 2014) and these practices should not be overlooked. The threshing floors should be cleaned properly before the operation so that there is no mixing of seeds or any insect-pests or pathogens. Harvesting and threshing should be followed by cleanliness of godowns to protect the seeds from the harmful pest and pathogens (Upadhyay and Ahmad, 2011). The storage godowns should be well lighted and the area should be preferably sunny, free from weeds and mostly situated at elevated areas. Damp areas should not be selected for building storage godowns as it will affect the moisture per cent of seeds thereby enabling easy growth of insect-pests and diseases (Parimala et al., 2013). Before bringing the new produce to the storage godowns, they should be cleaned properly. The cracks and crevices of storage godowns if any should be sealed ahead and the walls of the godowns should be whitewashed (Hazam and Kumar, 2022). The new seed lots should not be mixed with the older lots. The floors should be disinfected, and it should be cleaned regularly.

Millets are a type of minor cereals found in the Poaceae family. Millets are often described as “Nutri-Cereals” since they contain more nutrients than commonly produced main cereals. They are an important part of human’s diet in the world. These are foods with a high nutritional content, gluten-free and have nutraceutical qualities. The adequate amount of protein (essential amino acid), micronutrients and phytochemicals are found in millets. The phytochemicals found in millet grains enhance human health by lowering cholesterol and phytates in the body. Millets are full of antioxidants, minerals including dietary fibre, iron, calcium and magnesium that are vital for battling a variety of diseases. These are beneficial to include in our daily diet because the grain is healthy and nutritious. They decrease blood pressure, prevent cancer and lessen the chance of tumour growth. The rich nutrient content in millet has many health advantages such as cancer, obesity, lower risk of cardiovascular disease, gastrointestinal issues, migraines and asthma. People utilise millet-based products to fight a variety of nutritional deficiencies and diseases.

Millets which are also known as “Shree Anna” are a type of grass with small seeds that belongs to the Poaceae family and are widely grown throughout the world. Millets have high levels of proteins, minerals, vitamins, and antioxidants that why they are known to be climate-change-resilient crops. Proteins are essential amino acids that the body cannot synthesize and are used as a nitrogen source during embryo development following germination. Millets are high in dietary fibre and are important staple crop in many parts of the world. They are versatile grains that can be used to make a variety of food products, including millet flour. Millets are good for cardiac health, weight management, antioxidant activity, and may have anti-cancerous properties. They are nutrient- dense, provide sustained energy, and contain antioxidants such as polyphenols and flavonoids. Millets are considered safe and have a variety of health benefits. Surprisingly, millets perform major non-millet cereals in terms of nutrition. They are particularly high in dietary fibres, minerals, antioxidants, phytochemicals, and polyphenols, all of which have broad-spectrum positive effects on human health.

Minor millets, that belong to members of the cereal grass family, are advantageous in areas where large crops like Bread wheat, Rice paddy, and maize are relatively ineffective because they have a wide range of genetic diversity and thrive in marginal environments. The varied soil types, temperatures, heat and light cycles, and rainfall patterns across India all support the cultivation of minor millets. Finger millet, barnyard millet, foxtail millet, proso millet, and many more types of minor millet are used in agriculture. The majority of those who depend on these millets for sustenance and income are small-scale indigenous farmers and dwellers of rainfed areas, mainly among impoverished tribal populations numbering in the millions. Coarse cereals,now a days known as Nutri cereals or nutraceutical flora and are no longer known as coarse cereals as a potential global answer to malnutrition and unrecognized hunger. Minor millets have long been an essential component of Indian tribal societies’ agricultural systems and practices. They have used little millets for both famine prevention and ethnomedicine since the beginning of time. This chapter focuses on how little millet could help people have access to food and nutrition security while reducing hunger and malnutrition.

Millions of people worldwide suffer from food insecurity and malnutrition, which are exacerbated by issues including pollution, overcrowding, climate change, and economic hardship. These problems highlight the necessity of changing food systems for better immunity and health since they lead to both undernutrition and overnutrition. Essential carbs, fiber, fats, and important micronutrients including iron, calcium, potassium, zinc, and magnesium are all found in nutrient-dense grains such as foxtail, kodo, proso, tiny, and pearl millets. These millets are widely accessible and have several health advantages. For example, pearl millet is high in amylose starch and contains essential minerals like phosphorus and magnesium, whereas proso millet is a good source of iron, calcium, potassium, and B-vitamins. B vitamins, iron, calcium, and zinc are all found in kodo millet, while finger millet is a great provider of calcium, iron and magnesium. The nutritional profile of millets is further improved by processing methods like milling, germination, extrusion, and fermentation. Prominent research organizations, including as ICRISAT, ICAR, and CGIAR, are actively working to conserve millet and create better types to combat chronic illnesses and malnutrition. Targeted improvements in disease resistance and nutrient content are now possible because to biotechnology advancements. For improved worldwide health outcomes, dietary patterns must nonetheless be changed and these nutrient rich grains incorporated into contemporary food systems, notwithstanding current advancements.

Millets are highly nutritious and play a significant role in many people’s diets around the world. They have traditionally been transformed into a range of products through fermentation and malting for centuries. In the era of sustainable development, we still lag in using millets as a primary source of nutrition even after knowing that they are rich in terms of nutrition and other essential elements as compared to cereals. Today we have lots of information regarding different millets; nutrition content, methods of preservation, fermentation, malting, its products etc. Foods made from millet are regarded as potential prebiotics because they can improve the availability or functionality of probiotics, which has major positive effects on health. Foods have been fermented in order to enhance their flavor, texture and palatability. Although fermentation can result in a variety of products, the most prevalent ones are ethanol, lactic acid, carbon dioxide and hydrogen gas (H2 ). Commercial applications for these materials include use in food, vitamins, medications and industrial chemicals. Additionally, fermentation increases the amount of protein present, improves the accessibility of carbohydrates and reduces antinutritional components like tannin and phytic acid. Even though it has some drawbacks, such as the potential for contamination with dangerous microbes, fermentation technology is proven to enhance the nutritional quality of food products. This chapter covers the millet fermentation technology’s effects on the nutritional composition of the millets, contemporary developments in fermented millet-based products and technological improvements examined in the millet fermentation technology.

Introduction

Millets, which are small-seeded grasses belonging to the Poaceae family, have a rich historical and cultural significance as they have been cultivated for thousands of years (Goron and Raizada, 2015; Wen et al., 2014; Cheng, 2018). They remain an important crop in various regions worldwide, particularly in developing countries where they serve as a staple food for millions, especially in rural areas. Millets offer several advantages as they are gluten free and provide valuable protein, fiber, and minerals, making them highly nutritious for both human consumption and animal feed. The diverse range of millets includes pearl millet (Cenchrus americanus (L.) Morrone), foxtail millet (Setaria italica (L.) P.Beauv.), proso millet (Panicum dichotomiflorum Michx.; syn. Panicum miliaceum Walter), finger millet (Eleusine coracana (L.) Gaertn.), barnyard millet (Echinochloa frumentacea Link), kodo millet (Paspalum scrobiculatum L.), little millet (Panicum sumatrense Roth), and more (Ahmad et al., 2018; Bora et al., 2019). These grains have a significant historical presence globally, with evidence of cultivation in ancient China, Greece, and Rome (Li et al., 2020a; Huan et al., 2022). In India, millets have maintained their importance as a vital crop for thousands of years and continue to be an integral part of the diet in many regions of the country today (Padulosi et al., 2015; Li et al., 2020b).

Due to their remarkable health benefits, such as their low glycemic index, rich amino acid profiles, and abundance of B vitamins and antioxidants, little millets, formerly hailed as nutri-cereals, are once again becoming important dietary staples. These crops, which include a variety of species like finger millet, foxtail millet, and barnyard millet, have stimulated innovation in the food business by producing value-added goods like rice analogs, noodles, and biscuits. Additionally, their medicinal qualities could help advance research on nutraceuticals. Small millets are essential to climate-smart agriculture because of their renowned resilience, which allows them to flourish on marginal soils and in difficult climates. Breeding efforts seeking to create climate-resilient, high-yielding, and lucrative cultivars can benefit greatly from their extensive genetic diversity, which is conserved in traditional landraces and farmer-managed cultivars. Even with their potential, small millets’ genetic development projects are still in their early stages, requiring concentrated efforts to find trait-specific donors and enhance breeding techniques. In addition to preserving their ethnobotanical and cultural heritage, the conservation of their genetic resources supports international initiatives for environmental sustainability and food security. Small millets have the potential to become a vital component of sustainable and nutritionally enhanced agricultural systems by incorporating cutting edge agricultural techniques and speeding up breeding improvements.

Millets categorized as cereal crops are rich sources of nutrients, mostly cultivated across arid and semiarid areas of the world, specifically in southeast Asia and Africa. Millets are easy to cultivate, demands minimal field input and have appreciable market values. Despite of great value of millets, their production is limited mainly because of climate change which triggers multiple abiotic stresses like drought, high temperature, salinity, etc. Even though millets can withstand the majority of abiotic stresses, it necessitates their further improvement to develop more resilience against unprecedented effects of climate change by incorporating or enhancing stress tolerance characteristics. It is currently not attainable to create stress tolerant and high yielding varieties through conventional breeding techniques and thus modern approaches like genome editing become inevitable to achieve this goal. Also, available data for genome sequences of millets in these years promotes genome editing. The technique has drawn tremendous attention recently due to its potential in precise modification of alleles and is considerable to speed-up crop breeding by targeting important traits with precision. Additionally, many stress-resistant genes are identified in related monocots and are reported to be orthologs of millets which could assist genome editing for developing stress resilience and productivity in millets. Above all, the technique has also gained legal acceptance and has potential to revolutionary counter climatic challenges.

Millets are an essential food in the world’s semi-arid and arid regions. These are effective energy providers. They provide dietary fibre, polyphenols, fatty acids, minerals, vitamins and protein. The production of tropical crops is highly risky due to the pest outbreaks and increased abiotic pressures triggered by the climate change. Despite the fact that conventional breeding methods have greatly enhanced crop yield and output, new strategies are needed to further enhance crop productivity in order to fulfil the world’s expanding need for the food. The use of site-specific nucleases like zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN) and clustered regularly interspaced short palindromic repeat associated proteins 9 (CRISPR-Cas9) are the genome editing technologies developing very fast. The advancement of plant genome research and its use in plant breeding are significantly aided by such achievements. CRISPR/Cas9 and related genome editing methods are emerging as appropriate methods to reduce the negative consequences of climate change and assure the long-term food security of tropical countries’ growing populations.

Millets have potential as both food and fodders crops. Foods made from millet are abundant in micronutrients and necessary amino acids for regulatory functions. Since, millets were traditionally grown under adverse conditions, a great amount of polymorphism was preserved, making millet crops naturally resistant to abiotic and biotic stresses. Using data-driven technology to increase genetic gain in breeding operations can improve traditional breeding. Artificial intelligence can be used to create varieties that are adapted to certain soil and climatic conditions. In order to achieve breeding goals, machine learning (ML) is crucial in data mining and analysis, providing information for decision-making. We have been able to generate information on interesting genes using data mining and allele identification methods. For the exploitation of the natural variation and the identification of target genes, advances in high-throughput sequencing for the detection of genetic variations as well as the role of phenomics in genomics-assisted breeding can be employed. QTLs and marker-trait correlations can be identified via genome-wide association analyses. Multiple loci and genes that control agronomic traits have can be studied by comparative and functional genomics. Model selection is crucial for ML algorithms in smart breeding, which depends on knowledge in genotype, phenotype, and G×E interaction to be handled through computer science.

The demand of day by day increasing population there is lacking of nutritious food to peoples all around world. Underutilized crops like small or minor millets are having more nutrition and providing calories up to 2000 to 3000 calories per person per day than our staple crops i.e wheat, rice, and maize etc. Small Millets are the most underutilized crop in the world possessing high nutrient-cereal qualities and potential for the improvement of food security, nation income and generate the employment for the rural peoples. Millets are rich in nutrients such as proteins, fibers, carbohydrates, fats, vitamin-B, and minerals like calcium, phosphorus, magnesium, iron, potassium, and zinc etc.They are highly nutritive crop, non-acid forming, glutan free and help in reducing blood sugar level, blood pressure, diabetes and cardiovascular diseases. They also help in preventing asthma and migraines sufferings and fighting malnutrition in children s and adults.S mall millets are considered the forgotten crop species because of lower yield compared to other millets i.e. Sorghum and Pearl millet and cereals i.e. rice, wheat, maize etc. Being a highly nutritive cereal crop, easy to digest, high fibers and low glycine-max content, environment friendly and prevent the human hazards small millets still remained neglected and underutilized crop. Out of 200 underutilized crops identified 29 are small millets species (NAS USA, ICUC). Considering the food demand of common peoples, emerging health consciousness and nutrient demand for food security emerged small millets to limelight because of ecologically sound to climate. Still, the harvest of crop demands combination of computational technologies to full-fill food needs of population. Recognizing the potential of small millets in improving the national income UN-FAO announced the year 2023 as International Year of Millets. This chapter focused on application of climate smart agricultural methods such as artificial intelligence for the improvement of small millets to achieve future food security.

Agriculture is the support for the sustainable health and economic growth of the country. With the frequent changes in climatic conditions and increase of global population, it is important to come up with new varieties and nutrient rich crops. In this context, underutilized and neglected millets, considered as “nutri-cereals” or “smart foods” due to better adaptation in diverse environmental stresses and tolerance to insect pests and diseases, also a staple crop for hunger-stricken areas. Millet production is limited by the lack of intensive farming and deployment of genetic tools for trait and nutrition improvement. The recent advancement in machine learning (ML) and Artificial Intelligence (AI) technologies are key to depict plant response to environmental perturbation in huge data sets. Therefore, precision agriculture or smart farming is necessary to explore the genetic makeup of the neglected millets. AI has a substantial potential to handle numerous challenges in the formation of knowledge-based farming systems. The next agricultural revolution would emphasize more on farm machinery using AI integrated with the Internet of Things (IoT). In Smart agriculture, deep learning facilitates fast and interesting data analysis and provides support to researchers and farmers by the outcomes generated from the analysis. Here, we emphasize on the advancement of AI and its application to monitor the underutilized and neglected millets quality, yield and disease assessment. This chapter summarizes how AI can contribute to the improvement of sustainable productivity and quality of underutilized and neglected millet.

Millets are the staple food cropsacross the globe. The ability to thrive in harsh conditions established them as most suitable for current climate adversaries. The nutritional richness of millet reminded the need for adding them to our food basket.Despite possessing several desirable traits their cultivation is limited to a few pockets across the globe.Though significant progress has been achieved by conventional and transgenic breeding programs inmillets, there is a need for rapid improvement to meet the current needs.In order to accelerate the pace of breeding efforts, several molecular biology and biotechnology tools are being deployed by plant breeders. In the meantime, genome editing techniques like CRISPR, TALENS, ZFNs, Base and Prime editing have revolutionized the methods of genome engineering among which CRISPR has emerged as the most rapid, convenient and effective mechanism. The availability ofwhole genome sequence information, identified candidate genesfor various traits and successful transformation methods of millets promoted the application of CRISPR and resulted in the improvement of several traits. Initially, its application has been limited due to the restricted policies across the globe but the gradual change in the policies promoted genome editing across several parts of the globe. Thus, the application of genome editing for millet crop improvement can bring back those ancient grains to our food basket.

Introduction
Fruits and vegetables are an important part of the human diet as they provide the necessary nutrition, essential minerals, vitamins, antioxidants, dietary fiber, etc., required for maintaining proper health. India is the second largest producer of fruits and vegetables in the world after China. Comparison of area under horticultural crops during 2011-12 and 2021-22 indicate substantial improvement from 18.5 million ha to 27.6 million ha with a simultaneous increase in production from 215.04 million MT to about 333.25 million MT respectively in the country within a span of just one decade (Table 1.1). The credit for this spectacular growth in area and production goes to the sustained efforts of the central and state governments for the launch of several farmers’/ growers’ welfare schemes viz., National Horticulture Mission (NHM), Horticulture Mission for Northeast and Himalayan states (HMNEH), Mission for Integrated Development of Horticulture (MIDH); R&D support by the ICAR and State Agriculture & Horticulture Universities, extension agencies/Krishi Vigyan Kendra and proactive farmers and orchardists. However, the increase in production has still not kept pace with the improvement in infrastructure for post-harvest handling, management, processing, and value addition. Various studies still indicate that under normal conditions more than 15-20% of the produce goes to waste from production to consumption which in monetar terms accounts for huge economic losses. A recent study sponsored by the Ministry of Food Processing Industries Government of India and conducted by NABARD Consultancy Services has indicated that about 19.33 million MT worth Rs 58013.71 Crores of fruit & vegetables goes to waste annually as postharvest loss (4.87-15.05%) in various channels from harvesting to consumption (NABCONS,2022). The condition becomes even worse when there is a glut in the market, road blockades, or seasonal vagaries, and farmers are forced to leave their produce in the field itself without harvesting or dispose it at throwaway prices. Such a situation can, however, be controlled by diverting thisproduce to processing for conversion into a number of value-added products for its later use in human consumption. Thus, processing and preservation of horticultural crops have now become a necessity rather than a luxury.

Introduction
Food Pipe Line refers to a post-harvest system that deals with ensuring the delivery of a crop/commodity from the time and place of harvest to the time and place of consumption, with minimum loss, maximum efficiency, and maximum returns to all concerned including the grower, processors, and consumer. The term ‘system’ represents a dynamic, complex aggregate of logically interconnected functions or operations within a particular sphere of activity. The term pipeline refers to the functional succession of various operations but tends to ignore their complex interactions.
Stages of the post-harvest system
The food commodities broadly undergo different stages/operations of postharvest systems right from harvest to consumption. However, the applicability of different stages varies with the type of crop. Different unit operations involved in the post-harvest system of food commodities are as under:
1. Harvesting
2. Threshing (food grains, pulses and oilseeds)
3. Drying (food grains, pulses and oilseeds)
4. Collection, Sorting, grading, packaging (Horticultural Crops)
5. Transportation and distribution
6. Storage (Godowns, cold stores, CA stores)
7. Processing

Introduction
Food preservation refers to an art or science which deals with the process of prevention of decay, deterioration or spoilage of food commodity to allow it to be stored in a safe condition for future use. All foods gradually undergo deterioration or spoilage from the time immediately they are harvested or manufactured. Some commodities spoil rapidly, while others can keep longer but for limited periods. The basic purposes of food preservation are:
1. Extension of shelf life of foods thus increasing the supply
2. Ensuring the availability of seasonal foods throughout the year
3. Adding variety to the diet
4. Saving time by reducing preparation time and energy
5. Stabilizing farm prices and prices of food
6. Improving the nutritional qualities The food spoilage can be caused due to growth and activity of micro-organisms, insects or rodents, action of enzymes, chemical reactions and physical changes that takes place during processing, drying, or freezing etc. Classification of Food Depending upon the type of spoilage and level of perishability, the foods are classified into three broad categories:

Introduction: Food processing involves different steps from receiving raw material, preparing for product manufacture, and packaging to storage. All these steps are called unit operations. They are broadly categorized as raw
material preparation (receiving, sorting, grading, washing, peeling, blanching, slicing, cutting, dicing etc), processing (canning, drying, pulping, juicing, product manufacture like jam, jelly, preserves, candies, pickling, sauce making, etc.) and post-production handling including packaging and storage. A brief detail about each unit operation is discussed as under:
1. Raw Material Handling
Material handling includes varied operations such as hand or mechanical harvesting on the farm, transportation in trucks or refrigerated vehicles of perishable produce to the market or to the processing plant or to store/godowns. For conveying, a wide variety of mechanical conveyors is used depending on the type of material. Common conveyors used in the processing plant include screw conveyors, bucket conveyors, belt conveyors and vibratory conveyors. Throughout these operations, care is taken to maintain sanitary conditions, minimize bruises and product loss, maintain raw material quality like physical appearance, and vitamin contents, minimize microbial growth and minimize other detrimental changes to the product quality during handling etc. It also includes other unit operations like receiving, cleaning/washing, sorting, grading, peeling, halving, slicing, blanching etc for preparation of fruit and vegetables for processing.

Introduction
For setting up a fruit processing plant, the following cost and non-cost factors affecting the plant’s location are considered. Cost factors include raw material cost, transportation cost, cost of land, building and machinery, utilities cost, taxes, and insurance costs. At the same time, non-cost factors consisting of wages, salaries and incentives, market potential, community attitude, cost regulation, quality of life (school, living, recreation for workers, etc.), and environmental impact are taken into consideration. The main objective for the selection of the site for the processing unit is to minimize the sum of all costs. To minimize the cost, one should think not only the today’s costs but about long-term costs as well.
Plant Layout
The advantages of good plant layout are: -
i. Saving in floor space
ii. Increased output
iii. Fewer production delays
iv. Reduced material handling cost
v. Greater utilization of machine and manpower
vi. Easier and better supervision
vii. Less congestion and confusion
viii. Better appearance and more sanitary condition of work areas
ix. Reduced risk to the health and safety of employees

Introduction
Processing fruit and vegetables by applying heat is one of the most important methods of preservation as it exerts a positive preservative effect on the foods by destroying spoilage causing micro-organisms, enzymes, insects, parasites, etc. Heat processing also improves the availability of nutrients by improving digestibility, releasing vitamins like bound niacin, destroying anti-nutritional factors like trypsin inhibitor in some legumes including soybean, causing gelatinization of starches, and producing shelf-stable products. However, heating also causes some alteration or destruction of the sensory quality of fresh foods like changes in flavor, color, taste, and texture as a result, heatprocessed foods are perceived to have different sensory qualities than fresh produce.
Preservation by heat (thermal processing) involves heating foods in hermetically sealed containers for a specific time at a particular temperature to eliminate/kill/inactivate the microbial pathogens that endanger public health
and micro-organisms and enzymes that deteriorate food during storage. Most of the raw material used for canning is usually contaminated with different species and strains of bacteria, molds, and yeasts undergoing different stages of growth (lag phase, log phase, stationary phase, vegetative phase, sporulation phase, etc.,). Amongst these, bacterial spores are more difficult to inactivate. Thus, the criteria for heat processing chosen to inactivate the spores are also presumed to be capable of inactivating all other forms of microorganisms. Unlike fruits, most vegetables being low acid or even non-acid require a severe heat treatment to kill most bacteria. It is, therefore, necessary to work out the proper time and temperature combination for each category of food to ensure complete preservation.

Introduction
The term canning refers to a process in which the food is heated in hermetically sealed containers at a specific temperature for a specific time to eliminate microbial pathogens that endanger public health; and microorganisms & enzymes that deteriorate food during storage. The credit for the invention of canning goes to a French confectioner Francois Nicholas Appert, who was awarded a prize in 1809 by the French government for developing a new method of heat preservation of food in a sealed container and after whose name the process of canning has also been named as Appertization. In 1810 Nicholas Appert published the first book on canning entitled “The Art of Preserving Animal and Vegetable Substances for Many Years.” which is the first known work on canning. The work of Appert consists of packing food in glass bottles, closing them with corks, and heating the container in boiling water for several minutes depending upon the type of the food. Although the developed technique was used to preserve different types of food, the actual reason for the prevention of spoilage was known only after the work of Louis Pasteur (1864) who discovered that food spoilage was caused by microorganisms that were destroyed at elevated temperatures and the technique was known as Pasteurization. This understanding helped to form a scientific basis for establishing and revolutionizing the canning industry. Canning of fruits on a commercial scale was introduced in the USA in 1817 by William Underwood. The old phrase ‘necessity is the mother of invention’ suits well for the development of the canning industry during wars. The Civil War in America and the Great European War of 1914 also gave impetus to the development of a canning industry to meet enormous requirements of food for the fighting forces.

Introduction
Fruit juice and beverages comprise naturally extracted juices, drinks, ready to serve (RTS) beverages, carbonated juice drinks, nectars, squashes, cordials and appetizers, etc. These products are highly refreshing, thirst-quenching, appetizing and nutritionally superior to many synthetic and aerated drinks. Fruit juice is the natural liquid expressed from the edible portion of the fruit. Fruit juices are generally extracted from fruits in several ways, depending on their structure and composition. Soft fruits such as berries or tomatoes can be pressed in a fruit press or pulped by using a juicer attachment to a food processor. Citrus fruits are usually reamed to extract the clear juice. Harder fruits, such as pineapple, are peeled, pulped and pressed to extract the juice. Apple and pear fruits are crushed in a fruit grater and pressed in a hydraulic/ basket press to extract a clear juice. Passion fruit juice is prepared by using a pulper-finisher that separates the skin and seeds from the pulp. The fruits like mango, guava, apricot, peach etc from which the clear juice extraction is difficult are passed through the pulper to make pulp and then the pulp is utilized for preparation of juice, fruit drinks and ready to serve beverages. The composition of juice is unaltered during preparation and preservation while for fruit beverages like drinks, squashes, cordial etc the fruit juice or pulp, sugar, acid, colour, flavour etc are mixed in appropriate proportions to a desirable taste. Apple juice, orange juice, mango squash, guava drink, pineapple juice and squash, mixed fruit drink, mango nectar etc are the commercial products available in the market.

Introduction
The addition of sugar in foods acts as a preservative when its concentration is above 66%. It absorbs most of the available water and restricts the availability of moisture required for the growth of microorganisms. Sugar adds value to the product by imparting sweetness as well as body to these products. When sugar is boiled with acid and fruits, it is hydrolysed into glucose and fructose, the degree of inversion depending upon the pH and duration of boiling. Because of the inversion of sucrose, a mixture of sucrose, glucose and fructose is found in products like jam, jelly, marmalade, preserves and candies.
Jams, jellies and marmalades constitute an important class of fruit products preserved by using sugar as the method of preservation. Apple jam, mango jam, pineapple jam, strawberry jam and mixed fruit jam prepared by using the pulp of two or more fruits are quite common. Similarly, guava jelly and orange marmalade are also examples of such products.
A fruit preserve is made from properly matured fruit by cooking it whole or in the form of large pieces in heavy sugar syrup, till it becomes tender and transparent. A fruit impregnated with cane sugar and glucose, followed by subsequent draining and drying is called a candied fruit (Fruit candy) e.g. petha candy, ginger candy. While candied fruit covered or coated with a thin transparent coating of sugar, which imparts to it a glossy appearance is referred to as a glazed fruit. When candied fruit is coated with sugar crystals either by rolling it in powdered sugar or by allowing the sugar crystals from glucose syrup to deposit on it, it becomes a crystallized fruit (Crystallized Candy). The use of high concentrations of sugar in these products acts as a preservative. Products like Petha candy, aonla preserve, candied citrus peels, ginger candy, ginger in syrup, bael, pineapple and carrot preserve are the commercial products in this category.

Introduction
Many chemical substances are added to the food for functional purposes and in many cases, these are found to occur naturally in some foods. When these substances are added to the foods, they are known as food additives. An additive is defined as a substance or mixture of substances other than basic food ingredients that are present in a food as a result of any aspect of production, processing or packaging. Additives may be intentional as well as unintentional. Intentional additives are substances deliberately added to perform a specific function and to increase the shelf life of the products while unintentional additives have no intended function in the finished product but become part of that particular product. The food additives having significance in fruit and vegetable preservation and their mode of action are discussed as under:
Objectives of adding an additive
1. To reduce wastage and improve keeping quality e.g., the addition of potassium meta-bisulphite in squashes, benzoate in sauces, sodium propionate in bread and sorbic acids in cheese etc.,
2. To improve and maintain nutritive elements e.g., addition of vitamins for enrichment, addition of vitamin D to milk, addition of iodine to salt etc.
3. To enhance the quality and consumer acceptability of the product e.g., the addition of colouring agents, emulsifiers, thickeners etc.
4. To facilitate the preparation of foods e.g., the addition of acid to beverages, sugar in jams, jellies etc.

Introduction
Preservation by using common salt, spices and vinegar is a common method for the preparation of pickles, sauce and chutneys. Salt is mostly used as a preservative in pickles in combination with acid. Sodium chloride is used in brines and curing solutions or is applied directly to the food. The quantity of salt necessary to inhibit growth or harm the cells varies with the type of microorganisms. However, the minimum concentration of salt to act as a preservative is about 12%. Salt (sodium chloride) exerts its preservative action
by performing the following functions:
? Causing high osmotic pressure resulting in plasmolysis of the microbial cells.
? Dehydrating food by tying up the moisture thus making it unavailabl for the growth of microorganisms.
? Salt lowers the water activity and thus has adverse effects on microorganisms.
? Affecting the solubility of oxygen in the food and thus inhibiting the growth of aerobic microorganisms.
? Salt ionizes to yield chlorine ions, which is harmful to microorganisms.
? Chlorine in sodium chloride is a good oxidizing agent & is, therefore, toxic to microorganisms.
 

A. Tomato and Tomato Products
Tomato though botanically a fruit is generally considered a vegetable because of how it is consumed. Commercial products from tomatoes include juice, puree, paste, ketchup, soup canned and dehydrated tomatoes. Processing of tomato is now practised from home scale level to large scale enterprise. As a semi-finished product, tomato puree is prepared on a small scale while at a large scale, tomato paste has gained commercial significance. Both puree and paste are used for the preparation of different finished products like ketchup, juice, soup etc. The method for preparation of these products is well standardized, however, some modifications concerning recipes are made in the processing plants owing to variations in the quality of the raw material. The methods for the preparation of different tomato products are discussed as under: -
I. Tomato Juice/Pulp
Tomato juice is an unconcentrated product consisting of a liquid with a substantial portion of the pulp, expressed from ripe tomatoes with or without the application of heat and the addition of salt. Tomato pulp/juice is the basic ingredient for the preparation of different tomato-based products such as tomato puree, paste, ketchup etc. Fully ripe tomatoes are used for the preparation of tomato juice/pulp. All green, blemished and overripe fruits should be rejected as they adversely affect the quality of the product. The juice obtained from overripe tomatoes is usually thin and unpleasant. The juice should be deep red in colour with a characteristic taste and flavour. The juice shall have about 0.4% acidity expressed as citric acid. Cane sugar (1%) is added to further improve the taste and flavour of the finished product.

Introduction
Drying is probably the oldest method of food preservation used for agricultural crops, including food grains, oilseeds, spices, as well as fruit and vegetables. Drying refers to the removal of moisture content from the food to a level at which the activities of food spoilage micro-organisms are inhibited. Therefore, the reduction in water activity of the food as a result of moisture removal is themain principle of preservation by drying. Two commonly used terms for dried foods include low-moisture foods and intermediate moisture foods (IMF). Dried foods or low-moisture foods generally do not contain more than 25% moisture and have a water activity (aw) in the range of 0 to 0.6. Intermediate moisture (IMF) foods on the other hand contain 15- 50% moisture with a water activity (aw) of 0.60 -0.85. Dried grapes (raisins and munacca), apricots, dates, Morchella (Guchhi), bael, chilli, figs, etc. are commercial products prepared by using drying. However, the temperature used for drying is usually insufficient to cause the killing of microorganisms and enzymes, therefore any increase in moisture content during storage due to faulty packaging can also spoil the product. Thus, greater care is required for the packaging and storage of dried products.

Introduction
Freezing is one of the oldest and most widely used methods of food preservation, which allows the preservation of taste, texture, and nutritional quality in foods better than any other methods. The freezing process is a combination of the beneficial effects of low temperatures at which micro-organisms cannot grow, chemical reactions are reduced, and cellular metabolic reactions are delayed. Freezing is generally referred to as a unit operation in which the temperature of food is reduced below the freezing point and a proportion of water undergoes a change in state to form ice crystals. Immobilization of water to ice and the resulting concentration of dissolved solutes in unfrozen water cause a lowering of water activity of the food. Thus, reduction in water activity and use of low temperatures coupled with some pre-treatments like blanching is the basis for food preservation by freezing. Fruits such as strawberries, oranges, raspberries, black currants, and vegetables like green peas, green beans, sweet corn, spinach, carrots, sprouts, and potatoes are examples of commercially frozen fruits and vegetables.
The frozen food market is one of the largest and most dynamic sectors o the food industry. Despite considerable competition between the frozen food industry and other sectors, large quantities of frozen foods are being consumed all over the world. The frozen vegetable industry mostly grew after the development of scientific methods for blanching and processing in the 1940s. The commercialization history of frozen fruits is older than frozen vegetables. The commercial freezing of small fruits and berries began in the eastern part ofthe United States in about 1905.

Introduction
Plantation crops are high-value industrial crops grown in the tropics and coastal areas. They have great potential for utilization of wasteland like rainfed dry land, hilly, arid, and coastal areas to provide nutritional security; earn foreign exchange, and ensure livelihood security. The major plantation crops grown in India are tea (Camellia sinensis L), coffee (Coffea arabica L.), rubber (Havea brasiliensis), coconut (Cocos nucifera), areca nut (Areca catechu L.), oil palm (Elaeis guinensis), cashew nut (Anacardium occidentale L.) and cocoa (Theobroma cacao L.). India is the leading producer of most of
these plantation crops. Among all fruit crops, processing plays an important role in plantation crops as they are generally consumed only after processing. In addition to primary products, some by-products from these crops also have various commercial uses. Coconut, areca nut, cashew nut, black pepper, and cardamom are known as smallholder plantations whereas, crops like rubber, tea, and coffee are known as estate crops or conventional plantation crops. Processing aspects of some of these crops are discussed here:

Introduction
The food that has been damaged or has undergone changes so as to render it undesirable/unacceptable/unfit for human consumption is termed as spoiled. Spoilage of processed products is mainly caused by the action of micro-organisms and due to the physical or chemical changes. The reasons for microbial spoilage include under processing, inadequate cooling, contamination from leakage through can seams and pre-process spoilage. In under-processed cans (receiving low heat treatment), large number of different types of micro-organisms are expected to be involved in spoilage.
Causes of Food Spoilage
Food spoilage or deterioration may be described as loss of weight, softening, souring, rotting, wilting, moulding or a combination of one or more forms. The major causes of food deterioration are: biotic and abiotic factors.
A. Biotic Factors
1) Microbial spoilage (yeast, mould, bacteria)
2) Enzyme action (activities of enzymes present in foods)
3) Insect and rodent damage (attack of insects, rodents and parasites)

1. Introduction
Quality makes the product what it is. Quality is how well a product or service satisfies the needs of the customer. Quality can be interpreted in several ways, such as conformance to the standards, meeting customers’ preference/ satisfaction for desired quality attributes, degree of excellence, zero-defect products, etc.
The quality standard of fresh and processed fruit or vegetable products varies with their intended use. For marketing purposes, size, attractiveness, maturity, organoleptic quality and freedom from defects are to be kept in mind in fresh and processed fruit or vegetable products. During the processing of fruit or vegetables into value-added products, colour, flavour and texture also become important. Therefore, Quality is defined in different ways, such as:
i. Quality is a measure of the degree of excellence or degree of acceptability by the consumer.
ii. Quality is also defined as the combination of attributes or characteristics of the products that have significance in determining the degree of acceptability of the product to a user.

Introduction
Food laws and regulations are the legal standards of quality that regulate the manufacture, storage, distribution, sale, and marketing of food products. The major objectives of enacting the food laws and regulations are i) to maintain the quality of food produced ii) to safeguard the health of the consumers iii) to prevent exploitation of the consumer by the sellers and iv) to establish a standard of quality for different food products. In 2006 the government of India enacted the Food Safety and Standards Act, 2006 (FSSA) to consolidate all laws relating to food and to establish the Food Safety and Standards Authority of India (FSSAI) for laying down science-based standards for different articles of food and to regulate their manufacture, storage, distribution, sale and import to ensure availability of safe and wholesome food for human consumption. Various food laws enacted in our country before 2006 for different categoriesof food were:
• PFA (The Prevention of Food Adulteration Act),1954 (Act 37 of 1954)
• FPO (Fruit Products Order),1955
• Meat Food Products (Control) Order,1973
• Vegetable Oil Products (Control) Order,1998
• The Edible Oil Packing (Regulations) Order,1998
• Solvent Extracted Oil, De-oiled Meal and Meal and Edible Flour (Control) Order,1967

Introduction
In India, the government policy regarding the import and export of processed fruits is primarily regulated by the Agricultural and Processed Food Products Export Development Authority (APEDA), which requires exporters to register with them to export notified processed food items, including processed fruits, and implement various export promotion schemes to facilitate this trade; the overall goal is to boost the export of agricultural and processed food products through the Agriculture Export Policy (AEP).
1. Key features ofAgriculture Export Policy (AEP)
i. Governing body: APEDA, established under the Agricultural and Processed Food Products Export Development Authority Act, 1985.
ii. Registration required: All exporters of processed fruits are required to register with APEDA to export their products.

The following equipment and types of machinery are generally used in the food processing plant which specifically deals with the processing of fruits and vegetables;
I. Machinery/Equipment for general purpose
1. Weighing Balance (Precision balance for chemical and heavy duty for fruits/raw material) 1-500mg, 1kg,10kg,100kg.
2. Cutting Knife (SS), coring knife, pitting knife, peeling knife
3. Abrasive peeler (carborundum crystal) for potato,carrot,ginger etc.,
4. Working Table with Stainless steel/Aluminium top
5. Buckets/ Water tubs/jugs (Plastic/Stainless Steel)
6. LPG/Diesel Bhatti/furnace/Stove
7. Utensils/Bhagona (Aluminium /Stainless steel); 10, 50,100 kg capacity
8. Spoon, ladle (heavy duty), grater,`
9. Belt conveyors

Fruit firmness is an important indicator of maturity. During ripening, fruits may soften and become too soft at the overripe stage. The changes are detected by pressing the fruit in between hands or by using devices like a pressure tester or texture analyzer to measure the texture of fruits and vegetables. Commonly used pressure testers to measure the firmness of fruits and vegetables are the Magness–Taylor Pressure Tester and Penetrometer. Principle: For the measurement of pressure, the maximum force required to rupture the sample is measured by pushing a cylindrical metal probe into the sample to a given depth. The Magness-Taylor pressure tester can be used to determine the softening of fruits during maturity. In this instrument 10 lb or 30 lb force springs are available. Each of them is provided with one or two punches of 5/16 or 7/16 inch in diameter. The plunger of larger diameter is used on softer material, while; the smaller one is used for firmer material.
Procedure
1. Select the pressure tester according to type of fruit
2. Remove a thin portion of the skin in case of waxy fruits

The estimation of moisture in foods is one of the most important and most widely used measurements in the processing and testing of food products. It is used to determine the nutritive value of food, to express results of analytical determinations on a uniform basis, and to meet compositional standards or food laws. The level of moisture in different fruits and vegetables varies widely. Most fresh fruits contain more than 85-90% moisture in edible portions. Broad range of moisture content in common fruit, vegetables and their products as general guideline are given in Table 3.1.
Estimation of Moisture content in food products: Moisture content in foods can be estimated by following methods.
i. By Drying in an oven
ii. By distillation with an immiscible solvent
iii. By Chemical and physical methods (Infra-red heating method)

Measurements of sugars in the fruit can indicate the stage of maturity. Total soluble solid (TSS) contents can give a reliable measure of the sugar and other water-soluble components in fruits, vegetables, and their products such as juice, squash, jam, preserve, candy, sauce, ketchup, etc., In climacteric fruits, carbohydrates accumulate during maturation in the form of starch, and as the fruit ripens the starch is broken down into sugar. In non-climacteric fruits, sugar tends to accumulate during maturation. The presence of sugars, acid, ascorbic acid, and other water-soluble components in the fruit, vegetable, and their products are measured as total soluble solids.
Principle
Soluble solids can be determined using a refractometer which measures the refractive index or a brix hydrometer which measures specific gravity. A Brix hydrometer is used to determine the percentage of brix in syrup while an Abbe refractometer gives both refractive index as well as degree brix. The Refractive index of the test solution is measured at 20°C using a refractometer and the soluble solid content is calculated by the use of tables correlating the refractive index with soluble solids.

The inorganic residue remaining after the destruction/burning of organic matter in foodstuffs is called ash. Ash contents in food commodities roughly represent the mineral contents, however, some minerals are likely to be lost due to volatilization or some interaction between constituents during ashing. Estimation of ash content is also used as a measure to judge the quality of fruit products. The presence of high ash content or low alkalinity of the suggests the presence of adulterants in the sample. Similarly, the presence of a high amount of acid-insoluble ash indicates the presence of sand and other silicious matter in the food products.
Principle
Organic matter is burnt off at as low a temperature as possible. Heating is done in stages, first to char the product thoroughly and finally to ash it at 550ºC in a muffle furnace. The inorganic material left after burning organic matter is cooled and weighed.
Apparatus
* Silica Crucibles
* Muffle furnace
* Desiccator
* Balance

Most of the fruit, vegetables, and their products contain acid or a mixture of acids. These acids are mainly responsible for the tartness or sour taste. The level of acidity is estimated to measure the degree of tartness/sourness in the product.
Principle
Acidity in the sample is measured by titrating a known volume of sample against a standard alkali solution of known concentration using phenolphthalein as an indicator. The appearance of a light pink color indicates the endpoint of titration. However, for highly colored products like tomato sauce/ketchup, jamun juice, Rhododendron juice, mixed fruit jam, etc., accurate determination of endpoint may be difficult by using an indicator. Therefore, for such samples, acidity is measured by using electrometric methods i.e. titration to a predetermined pH value, or the sample is further diluted with distilled water to mask the interfering color. The level of titratable acidity is expressed in terms of the predominant acid. Different fruits, vegetables, and their products have different predominant acids. A list of some products having predominant acid is shown as under: