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By definition, minimal processing would encompass any procedure, short of traditional complete preservation procedures (heat sterilization etc.) that adds value to the fruit or vegetables (Floros, 1993). Other terms used to refer minimally processed products are lightly processed, partially processed, fresh processed, fresh cut and prepared. Minimally processed fruits and vegetables are products those have the attributes of ‘fresh-like’ quality. A number of minimally processed products of fruits and vegetables are now being produced like peeled and sliced potatoes, shredded lettuce and cabbage, washed and trimmed spinach, chilled peach, mango, banana, melon and other fruit slices, vegetable snacks such as carrot and celery stick  and cauliflower and broccoli florets, packaged mixed salads, cleaned and diced onion, peeled and cored pineapple, fresh sauces, peeled citrus fruits and microwaveable fresh vegetable trays. Minimal processing of fruits and vegetables has two purposes. First, it is important to keep the product fresh but convenient without losing its nutritional quality. Second, the product should have a shelf life sufficient enough to facilitate distribution within the region of consumption. The microbiological, sensory and nutritional shelf life of minimally processed fruits and vegetables should be at least 4-7 days but preferably even longer (Ahvenainen, 2000).

India is known as the food basket of the world. It is the second largest producer of fruit till now after China, but its contribution to the Global market of fruit processing is just 1per cent. This is due to the fact that there is deficiency of skilled manpower, poor cold storage facilities, inefficient post-harvest management and minimal technological interventions.
Deficiency of the skilled manpower: In-spite of market size of US dollar 181 billion, expenditure on food and food products being 21per cent of India’s GDP and more than 31per cent share of wallet by consumers, there is resultant apprehension about the emergency emerging skill shortages due to mismatch between the demand for specific skills and available supply. Shortage of skilled, Semi- skilled and unskilled workers has emerged as a critical factor impacting the competitiveness of Indian food industry. Efficient man-power is one such critical element to improve to foster the growth. As the food industry advances and adopts various levels of automation the demand of skilled manpower will increase phenomenally. Hence if, the Indian food industry must achieve projected growth target set and become globally competitive, the need to develop and train the human resource efficiently would be essential.

1. Peeling: It is one of the integral parts of a food processing, and most agri-cultural crops need to be peeled to remove at the initial stage of food processing Peeling removes inedible portion (peel, seeds, and stalk) of fruits and vegetables. However, the susceptibility to spoilage increases due to acceleration of physiological process and the exposure of the tissues to microorganisms. The shelf-life and quality of fresh-cu produces could be compromised with the peeling.
The goals of optimum peeling operation are i. Minimizing product losses, ii. Types of products e.g., potato products, iii. Minimizing heat ring formation e.g., apple, potato, iv. Minimizing energy and chemical usage, v. Minimizing the environmental pollution.
Peeling operation can be grouped under following categories: Manual peeling (knife or blade), Mechanical peeling (abrasive devices, devices with drums, rollers, knives or blades and milling cutters), Chemical peeling, Enzymatic peeling, and Thermal peeling (Flame or dry heat peeling, steam or wet heat peeling, thermal blast peeling, and vapour explosion or vacuum peeling.

1. Calibration of Milk Butyrometer

Introduction

Butyrometer is used for determination of fat for milk and milk products by Gerber method. The milk butyrometer is a graduated vessel, which are designed in such a way that the internal volume of each unit is 0.125 ml which is corresponding to 1 percent fat. Sometimes due to manufacturer’s error, the volume of one unit of milk butyrometer is not exactly equal to 0.125 which leads to subsequent error in results in milk fat. So before starting to use a new butyrometer, it is essential to calibrate for its efficiency in the laboratory. Mercury is used to calibrate milk butyrometer.

Apparatus

Milk butyrometer (0 – 10% graduation), analytical balance

Microbial contamination sources: Foods may be contaminated by microorganisms at any time during harvest, storage, processing, distribution, handling, or preparation. The primary sources of microbial contamination are soil, air, animal feed, animal hides and intestines, plant surfaces, sewage, and food processing machinery or utensils. Since most foods either carry or eventually acquire bacteria, molds, or yeasts, microorganisms are the major causes of food spoilage. The increased load of microorganisms leads to rapid spoilage.

Organisms: Bacteria and fungi (yeasts and molds) are the principal types of microorganisms that cause food spoilage and food- borne illnesses. The typical microorganisms that cause food spoilage are bacteria (e.g., Pseudomonas), yeasts (e.g., Candida), and molds (e.g., Rhizopus). The growth of microorganisms in the food will increase the load of microorganisms, and the heavier load will add to the difficulty of preventing spoilage and may influence the kind of spoilage to be anticipated.

Food spoilage may be defined as any change that renders food unfit for human consumption. These changes may be caused by various factors, including contamination by microorganisms, infestation by insects, or degradation by endogenous enzymes (those present naturally in the food). In addition, physical and chemical changes, such as the tearing of plant or animal tissues or the oxidation of certain constituents of food, may promote food spoilage. Foods obtained from plant or animal sources begin to spoil soon after harvest or slaughter. The enzymes contained in the cells of plant and animal tissues may be released as a result of any mechanical damage inflicted during postharvest handling. These enzymes begin to break down the cellular material. The chemical reactions catalyzed by the enzymes result in the degradation of food quality, such as the development of off- flavours, the deterioration of texture, and the loss of nutrients. The enzymatic changes in foods are desirable to some extent as in the case of ripening of fruits, but continued enzymatic activity beyond an optimum point becomes food deterioration due to rotting. The native enzymes may be inactivated by heat, radiation or by the use of specific chemicals.

10.1 CEREALS AND CEREAL PRODUCTS

CONTAMINATION: The sources of contamination in cereals and cereal products are soil, insects, air, etc. Most of the microorganisms are removed with the outer portions of the grain during milling. The bleaching agents used during milling also reduce numbers of microorganisms. The bacteria in wheat flour include Bacillus, Micrococcus, Flavobacterium. Mold spores are chiefly of Aspergillus spp and Penicillium spp and in addition Alternaria, Cladosporium and other genera are found. The numbers of microbes in cereals and cereal products vary widely from few hundred per gram to million. Corn meal contains several hundred to several thousand bacteria and mold per gram.

2.1 Introduction
Typically, milk is obtained from domesticated animals (water buffalo, sheep, or goats) under farm-level circumstances. In smaller amounts, it can be utilized directly after boiling; but, in larger quantities, processing and storage are crucial. Farms provide their milk to milk vendors, who collect it in cans and deliver it to chilling centers or dairy processing plants. After milking, the milk is quickly chilled to 4°C, or it should be pasteurized and packaged within two hours. Before being marketed, milk must be pasteurized, with the exception of products that initially require raw milk or those that require direct application of heat, such as desiccation or another heating process. The market for novel goods and procedures is steadily growing. The main causes include rising disposable incomes, shifting consumer attitudes and concerns about safety, hygiene, and nutrition, and the entry of foreign companies.

2.2 Dairy plant requirements
The plant should be situated in an area free from waterlogging, well- protected, easily accessible by car, and equipped with a plentiful supply of drinkable water, a facility for disposing of waste, and energy.

2.2.1 Infrastructure requirements
For the receiving of milk, chilling, storing, separating, pasteurizing, homogenizing, packaging, and product manufacturing, civil works with appropriate space and machinery are needed. provisions for utility units such steam generation and refrigeration control, storage, and quality control. When choosing metals for equipment in dairy plants, look for materials that are nontoxic, tainting, corrosion-resistant, easy to clean, affordable, and long-lasting. Generally, alloys made of aluminum or 18:8 stainless steel are recommended.

The lack of mechanistic understanding of probiotic activity is a major drawback for the prediction of safety of probiotic intervention. The complex gut-associated microbial ecosystem and nutritionally related factors are the most important environmental triggers for the development and modification of chronic degenerative diseases including immune and metabolic pathologies. In fact, the interaction between the gut microbial ecosystem and the host is considered to be a critical factor in overall health or disease. Whether probiotics will exert protective, harmful, immune-stimulating or immune-suppressing effects on a host is dependent on the interaction between the microbial signals, the genetic make-up of the host and the effects by the environment. Many studies have revealed that certain probiotic bacteria stimulate immune cell proliferation and activity as evidenced by the production of cytokines and antibodies, thereby enhancing the effectiveness of the immune response against pathogens. Ng et al. (2009)

4.1 Introduction
At the moment of secretion in the udder, milk is sterile; however, it is contaminated by bacteria before it is extracted from the udder. With the exception of mastitis, the bacteria are generally innocuous and restricted at this juncture. Microorganisms may contaminate the milk further during milking, handling, storage, and other pre-processing activities. The bacteria have the potential to either enhance the quality of milk or cause it to deteriorate. Some of the beneficial bacteria, particularly the lactic acid bacteria, are referred to as probiotics. These probiotics are gaining a more prominent position in nutraceutical dairy and other food products, which provide consumers with health benefits such as the control of diarrhoea, antihypertensive, hypocholesterolemia, immunostimulatory, and anticarcinogenic properties.

4.2 Lactic acid bacteria
This particular group of bacteria is capable of homofermentation, which is the process of fermenting lactose to lactic acid, and heterofermentation, which is the process of generating a high number of end products. They are commonly present in milk and are also used as starter cultures in the production of cultured dairy products, such as yoghurt.

Veterinary public health deals with the contributions of veterinary medicine to public health. It is concerned, in the first instance, with the control and elimination of zoonoses and also with the following areas of activity:
    ·    Food hygiene (production, processing and distribution of foods of animal origin)
    ·    Environmental contamination and degradation by animal wastes and animal products
    ·    Comparative medicine (eg. Studies in animals on naturally appearing cancer, cardiovascular disease, metabolic and other disorders encountered in humans)
    ·    Laboratory animal medicine
    ·    Education and training in VPH and communication with the public
    ·    Reproduction biology
    ·    Aspects of mental and societal health
    ·    Emergency veterinary functions in natural disasters and other catastrophes.

Antibiotic Resistance and Gene transfer
 
It is important that probiotic strains should be assessed for their phenotypical antibiotic resistance and potential to transfer resistance gene as otherwise they cannot be used in food (BgVV, 2000; Vankerckhoven et al., 2008). It is vital to do research on characterization of acquired resistance mechanisms and transferability of resistance genes and on methods for determining transferability.

5.1 Introduction
The process of milk processing entails the transformation of liquid milk into dairy products, including pasteurised liquid milk, yoghurt, butter, cheese, and ghee. The following are the rationales for processing: There are numerous benefits to processing milk, including the ability to access distant markets, the extension of the product’s storage life, and the attraction of higher prices because of its enhanced transportability. Furthermore, processed products are less cumbersome and lighter, which facilitates transportation. The refining of milk improves its quality and hygienic safety, enabling the production of a variety of milk products, including cheese and liquid milk, to satisfy the diverse needs of consumers. Additionally, the processing industry contributes to economic development by creating employment. Small-scale processors are capable of producing a wide range of dairy products. Before determining which dairy products the group will produce, it is recommended that a comprehensive market/feasibility study be conducted. Milk products can be processed in the manner depicted in the image.

In spite of the problems with dosage and viability of probiotic strains, a lot more work is required for industry standardization and safety issues. However, it is noted that probiotics have considerable potential against many pathogenic and other diseases. A wide range of clinical trials are still needed to standardize and optimize the conditions. Ongoing basic research will continue to identify and characterize existing strains of probiotics, identifying strain-specific outcomes, determine optimal doses needed for certain results and assess their stability through processing and digestion.

Food extrusion technology is a popular means of preparing snacks and ready-to-prepare food items. It is a method of size enlargement in which tiny granular food or powdered particles are strengthened into bigger parts with various forms, texture, color, etc. Extrusion cooking or thermoplastic extrusion is a common extrusion technology considered to be a process of HTST (high temperature, short time). It allows the manufacturing of a wide range of food products with little or no alteration of the fundamental equipment and adequate process control. Extrusion cooking is used for starchy and protein substances to make nutritious foods. Generally, such products are rich in calories and incorporation of protein rich fish improves its nutritional value. Most of the cereal-based extruded snacks were primarily made from maize, wheat and rice. However, these cereals (e.g. rice) have comparatively low protein content (6 - 8 g/100 g db) and an elevated amino acid profile in glutamic and aspartic acid, whereas lysine is the restricting amino acid. Therefore, it is recommended that protein food ingredient (e.g. fish) be added to the extruded snack products to develop nutrient rich diets.

Fish is considered to be great sources of protein of high dietary importance with a balanced profile of essential amino acids and lipids containing omega-3 fatty acids, in particular essential fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), respectively. EPA and DHA are very essential to normal growth and development. To avoid or moderate coronary artery disease, hypertension, diabetes, arthritis, other inflammatory and autoimmune illnesses, these vital fatty acids are discovered. The benefits of fish-based extruded products will assist in providing a big population in the nation like India with nutritious and balanced diets.

Bacteriophage is the term applied to bacterial viruses. Bacteriophage having the ability to lyse certain sensitive living cells. Phages are small particles, which can only be seen in the electron microscope after the application of suitable staining techniques. All phages have a head and most have a tail. The head may be isometric or prelate with a hexagonal outline.

There are two types of phages virulent and temperate which enter a different relationship with the host, the lytic and lysogenic cycles respectively.

14.1 Introduction
India is now the top producer of milk globally, accounting for 13.1% of the world’s total milk output. Fresh milk, if left at room temperature, will spoil within a few hours. To extend its shelf life, some of the fresh milk can be converted into fermented products. This fermentation process not only prolongs the product’s shelf life but also enhances its flavor and makes it easier to digest. Historical evidence suggests that people have been making fermented milk products since as far back as 10,000 B.C. Various strains of Lactobacilli have been cultivated in labs to create a diverse array of cultured milk products with unique flavors. Since as early as 10,000 B.C., milk has been utilized to produce fermented dairy items in different parts of the world. These fermented products offer benefits such as improved digestibility, distinctive tastes, added probiotics, vitamins, minerals, and extended shelf life. There is an enormous diversity of fermented dairy products globally, with each product’s characteristics influenced by the local strains used in the fermentation process.
14.2 Lassi
In India and Pakistan, lassi is a somewhat common beverage. It is produced by combining Indian spices with water or milk and curd or yogurt. Sweet lassi is made with sugar or fruits rather than spices; traditional lassi, sometimes called salted lassi, or just lassi, is a savory beverage occasionally seasoned with ground roasted cumin.

K ration: It is a lightweight packaged ration of emergency foods developed for the U.S. armed forces in World War II (Plate 215).

Kairomone: A chemical substance emitted by one species and especially an insect / plant that possess an adaptive benefit (stimulus for oviposition) to another species.

Kaiser rolls: A round crusty roll often used for sandwiches (Plate 216).

Kalamata/calamata: It is brine cured black olive grown in Greece.

Kallikrein: A hypotensive protease that liberates kinins from blood plasma proteins and is used therapeutically for vasodilation.

Kanji: Clean, black coloured, crisp and sound product of characteristic flavor prepared by full fermentation (chiefly lactic acid fermentation) of black carrot with 2.5% salt, 1% powder mustard and some amount of water.

Kaposi’s sarcoma: It is a neoplastic disease that occurs in individuals coinfected with HIV and a specific herpes virus (species Human herpesvirus 8 of the genus Rhadinovirus), that affects skin and mucous membranes, and is marked by pink to reddish-brown or bluish plaques, macules, papules, or nodules.

Tuberculosis

Tuberculosis has been recognized as one of the most important infectious diseases in the world because of its high global impact and its chronic debilitating character, particularly in the poor section of society. Milk-borne tuberculosis is directly or indirectly related to the consumption of raw milk from infected dairy herds.

Normally raw milk contains different levels of inhibitory substances, which restrict the normal development of bacteria and some times may even kill them. The degree of inhibitory activity varies considerably in milks of different species, viz, cow, buffalo, human. Maximum activity present in human milk, variations occur in the milks of individual animals and some times even at different milking or from different quarters of the udder of the same animal.
There are two groups of antimicrobial substances present in milk:
    1.    Inherent or naturally occurring substances in milk. 
    2.    Non- inherent compounds secreted into milk from other sources.

Introduction

Fisheries and aquaculture are important source of food, nutrition and livelihood for millions of people around the world. Aquaculture is the fastest growing food-production sector in the global aquaculture with production of 73.8 Million tonnes in 2014 (FAO, 2016) expanding into new directions, intensifying and diversifying. A persistent goal of global aquaculture is to maximize the efficiency of production to optimize profitability.

Infectious diseases pose of the most significant threats to successful aquaculture. Intensively cultured fish and shellfish are susceptible to infectious agents. The losses due to shrimp diseases in India is valued at Rs. 10, 221 million. Globally, the yield loss due to diseases in aquaculture is estimated to be $ 6 billion. A variety of antimicrobial and chemical treatments have been used for the control and treatment of bacterial diseases in aquaculture systems. The emergence of antimicrobial resistance in bacteria due to application of antibiotics in aquaculture and the toxic nature of chemicals led to the search for alternatives for the control of infection. Here, we discuss the several alternatives to antibiotics that have been successfully used in aquaculture.