eChapters

A. Gram’s Staining
Introduction
The most widely used staining procedure in microbiology is the Gram stain, discovered by the Danish scientist and physician Hans Christian Joachim Gram in 1884. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives. The procedure is based on the ability of microorganisms to retain color of the stains used during the gram stain reaction. Gram-negative bacteria are decolorized by the alcohol, losing the color of the primary stain, purple. Gram positive bacteria are not decolorized by alcohol and will remain as purple. After decolorization step, a counterstain is used to impart a pink color to the decolorized gramnegative organisms.
The Gram stain procedure enables bacteria to retain color of the stains, based on the differences in the chemical and physical properties of the cell wall.
1. Gram positive bacteria: Stain dark purple due to retaining the primary dye called Crystal Violet in the cell wall. Example: Staphylococcus aureus
2. Gram negative bacteria: Stain red or pink due to retaining the counter staining dye called Safranin

1. Introduction

India is a land of large varieties of fruits and vegetables due to its vast soil and climatic diversity. India is the second largest producer of fruits and vegetables in the world, with an annual production of fruits 45.5 Million tonnes and vegetables 90.8 Million tonnes. It contributes 10.23% and 14.45% of the total world production of fruits and vegetables (F&V) respectively (NHB, 2010). Though fruits and vegetables are grown only on 7–8% of gross cultivated area, these contribute more than 18.8% of the gross value of agricultural output and 52% export earnings from total agricultural produce. These have a high export potential of 20-30 times more foreign exchange per unit area than cereals due to higher yields and higher price available in the international market (Sen et al., 2012). According to estimates, the per capita consumption of fruits and vegetables in India is only around 46g and 130g which are far below the stipulation of a minimum of 92g and 300g respectively as recommended by the Indian Council of Medical Research and National Institute of Nutrition. The low availability is mainly due to considerably high post-harvest losses. About 15-20% of the fruits and vegetables are wasted every year in the country, due to poor post-harvest management and lack of infrastructural facilities for processing and storage, amounting to a revenue loss of over Rs. 625 billion.

The sensorial, nutritional, and organoleptic quality of fresh produce starts deteriorating after harvest as a result of soon after harvest the altered plant metabolism and microbial growth. The quality deterioration is the result of produce respiration and transpiration, senescence, ripening-associated processes, wound-initiated reactions, development of postharvest disorders, microbial proliferation leading to postharvest quality loss. Therefore, it is necessary to understand the respiration and ripening processes and the physiological and biochemical changes during ripening. The best way to reduce respiratory metabolism is to reduce the storage temperature. All biological processes proceed more slowly at lower temperature (Yahia, 2007). In general, O₂ levels must be reduced to less than 10 % to see a reduction in the rate of respiration. Respiration rate is temperature dependent; so, as the temperature reduces the amount of oxygen required also drops. Therefore, oxygen levels must be substantially reduced at low temperature for it to affect the rate of respiration. It must however, be ensured that O₂ levels do not fall below extinction point as anaerobic respiration and decay may occur. Tolerance to low O₂ varies between species and cultivars and is also time dependent. Commodities may be able to tolerate low O₂ for short periods of time. Low O₂ can also be tolerated when CO₂ is low.

Materials Required

Fruit, pulper, fruit mill, hydraulic press, screw type juice extractor, stainless steel pans, chemical preservatives, glass bottles, closures, crown corks, crown corking machine, plastic barrels.

Procedure

    1.    Select and weigh sound fruits/vegetables for extraction of juice/pulp.    
    2.    Wash and peel the fruits as required.
    3.    Extract the juice by hot or cold process.
    4.    Pasteurize the juice/pulp to inactivate the enzymes and partially kill microorganisms.
    5.    Preserve the juice/pulp by heat processing after hot filling and hermetic sealing or by addition of permissible quantities of suitable preservatives.
    6.    Label the bottles properly and keep them for storage

EXPERIMENT

TECHNOLOGY FOR EXTRACTION OF KERNEL OIL FROM STONE FRUITS

Apricot, peach, plum, cherries, nectarines etc are the important stone fruits belonging to the family Rosaceae. These fruits are known to contain stones/pits i.e., apricot (6.0-11.5%), peach (7.5-11.5%), plum (4.5-7.0%) and these stones are reported to contain kernels (%) apricot (28.0-32.0%), peach (5.7-5.9%), plum (10.0-20.8%), almond (28.0-30.0%). These kernels can yield an oil yield of (42.5-45.5%), (38.5-41.5%), (39.2-40.6%) and (52.0-58.9%), respectively. Thus, these stone fruit kernels can be utilized for extraction of kernel oil by using the improved technology developed for extraction of oil. The developed technology consists of mechanical decortication of stones by the use of mechanical decorticator, kernel separation by using specific gravity separation technique, oil extraction by using table oil expeller and oil filtration through oil filter press followed by packing in PET bottles and PE pouches.

Mechanical decorticator: The mechanical decorticator is a type of roller crusher consisting of two rollers moving in inward opposite directions with the help of 3 HP motor and is provided with a hopper and feeder assembly. The clearance between two rollers is adjusted according to the respective size of stones.

Table oil expeller: Oil expeller is a 24 patti screw type oil expeller driven by 7HP motor, in which the kernels are fed into the moving hopper at pre-determined flow rate, which are pressed in between rollers (worm) and side walls of the expeller. The kernels are then, passed through the expeller 3-4 times until a thin slice of press cake is obtained.

1. Algal Biomass

Algae is defined as heterogeneous assemblage of organisms that range in size from tiny cells to giant seaweeds, thus algae mostly are photosynthetic species which include eukaryotes and prokaryotic Cyanobacteria (blue-green algae) and live in aquatic habitats. They are ubiquitous in nature and are found in variety of environments ranging from soil, sand, marshes, brackish water, sea and fresh water. Most of them are capable of manufacturing their own food.

Microalgal research is offering wide potential in food biotechnology and is gaining more attention because of presence of wide range of bioactive and nutraceutical properties and added health benefits.The increasing population is one of the major growing concerns of today’s world. To meet the nutritional requirement of the rising population, researchers are looking for optional sources for food which are easy to cultivate, cost effective and produce large amount of bioactive compounds useful to prevent major diseases. Chlorella is unicellular green alga that is a potential source of nutrients like carotenoids, minerals, vitamins, and polyunsaturated fatty acids. Especially, omega-3 fatty acid and carotenoids have received considerable interests due to their roles in the prevention of chronic diseases. Lutein and zeaxanthin from Chlorella have been shown to be associated with eye health and function. Beta-carotene is known to reduce the risk of CVDs and certain cancers. Animal studies also demonstrated that Chlorella supplementation reduced the serum cholesterol levels.

Similarly, the blue green microalgae Spirulina, possesses diverse biological activities and nutritional significance. Regular consumption of Spirulina fortified food improves immune system by increasing phagocytic activity of macrophages, stimulating the production of antibodies and cytokines, increase accumulation of NK cells into tissue and activation and mobilization of T and B cells. Spirulina have also shown to perform regulatory role on lipid and carbohydrate metabolism by exhibiting glucose and lipid profile correcting activity in experimental animals and in diabetic patients. They were also found to be active against several enveloped viruses including herpes virus, cytomegalovirus, influenza virus and HIV. They are capable to inhibit carcinogenesis due to antioxidant properties.

Food extrusion technology is a popular means of preparing snacks and ready-to-prepare food items. It is a method of size enlargement in which tiny granular food or powdered particles are strengthened into bigger parts with various forms, texture, color, etc. Extrusion cooking or thermoplastic extrusion is a common extrusion technology considered to be a process of HTST (high temperature, short time). It allows the manufacturing of a wide range of food products with little or no alteration of the fundamental equipment and adequate process control. Extrusion cooking is used for starchy and protein substances to make nutritious foods. Generally, such products are rich in calories and incorporation of protein rich fish improves its nutritional value. Most of the cereal-based extruded snacks were primarily made from maize, wheat and rice. However, these cereals (e.g. rice) have comparatively low protein content (6 - 8 g/100 g db) and an elevated amino acid profile in glutamic and aspartic acid, whereas lysine is the restricting amino acid. Therefore, it is recommended that protein food ingredient (e.g. fish) be added to the extruded snack products to develop nutrient rich diets.

Fish is considered to be great sources of protein of high dietary importance with a balanced profile of essential amino acids and lipids containing omega-3 fatty acids, in particular essential fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), respectively. EPA and DHA are very essential to normal growth and development. To avoid or moderate coronary artery disease, hypertension, diabetes, arthritis, other inflammatory and autoimmune illnesses, these vital fatty acids are discovered. The benefits of fish-based extruded products will assist in providing a big population in the nation like India with nutritious and balanced diets.

Extrusion cooking is an emerging technology having advantages of low production cost, capable of producing end products in various shapes and sizes, from a variety of food materials with easy digestibility, maintaining hygiene and high product quality and leaving no effluents or hazardous materials. Minimal energy consumption and processing costs and possibility of flavour addition are the added advantages of technology. The process/technique has the versality and ability to constituently produce a given product in continuity at a given set of pre – determined specifications. It destroys fat splitting enzymes to avoid rancidity and thus extends the shelf life of products considerably. Microorganisms present in food materials are destroyed. It can be effectively utilized to develop functional and designer foods by using several locally available under-utilized food materials. However, it leads to increased package cost as puffed or expanded food products have low bulk density. R & D studies are further needed for co – extrusion of variety of cereals with high fibre content foods and micronutrients.

1.1 Introduction and Background

In present scenario, the word “extrusion” refers to a process by which dry or semi-moist ingredients with varying in-barrel moisture are forced through varying barrel temperature, screw speed and screw configuration through a die opening of the desired cross section. In other words, extrusion is predominantly a thermo-mechanical processing operation that combines several unit operations, including mixing, coating, kneading, venting, shearing, heating, forming, partial drying or puffing, depending on the material and equipment used.

The origins of extrusion are in the metallurgical industry, where in 1797 Joseph Bramah patented the first extrusion process, wherein a piston driven device was used for making seamless lead pipes. The present understanding of extrusion technology and the developments or improvements in machine design are largely due to research carried out by the plastics industry. In food industry, this technology is in use since 1930 and its popularity is increasing continuously due to its efficiency, continuity and sustainability. In beginning, sausage extruders were developed in the nineteenth century as simple forming machines. One of the original developments which laid the foundation of the food extrusion was the use of pasta press termed as “cold extruder” in mid 1930s. Furthermore, in the late 1930s, General Mills, Incorporation., Minneapolis, MN, USA, first used a single screw extruder in the manufacture of ready-to-eat (RTE) cereals. Later in 1970s, twin screw extruders were used for the manufacture of moist pet-foods. Twin screw extruders were also adapted from polymer industry, as rising food quality and limitations of single screw extruders were demanding a more versatile process for the newer and higher quality products. After 1970s, the extrusion processing with simultaneous use of heat was popularized as a versatile and highly energy efficient with negligible wastage process. In all these years, number of changes has been noted in the extruder design such as changes in screw design, barrel design and also material used for the manufacture of these materials. Barrel liners availability along with already available segmented screws could further add more economics to the process and increased equipment lifetime. For better wearing characteristics, a number of screw elements of different materials are being used in addition to standard nitrided steel for customized processing needs, such as corrosion and wear resistant surface coated steels, stainless steel, satellite welded and bimetallic HIP (Hot Isostatic Press). JBL Feedscrews Limited, a UK based company recently introduced tungsten carbide coated screw in conjunction with a compatible bimetallic barrel. Likewise, a number of options for barrel metallurgy are available to withstand the higher internal pressure and temperature. Barrels lined with hard cobalt based alloy, typically Xaloy, to give abrasion and chemical resistance are also in use, although, the most common material is carbon steel with a nitride interior. Similarly, steam heated or jacketed barrels were used earlier for cooking purpose which gradually replaced by the electric induction heating elements. These HTST cooking extruders are versatile, highly productive and energy efficient processing machines. They can use a wide variety of raw materials and formulations to produce products with increased shelf-life and convenience.

F0 value: It is used to express F-value when z =10oC / 18oF and the temperature is 121.1oC. It is the number of minutes at a specific temperature (250oC) required to destroy or kill a stated number of microorganisms and has specific z-value. It is also called reference value.

Facing or lining board: The layer of the paper on which the flute is embedded is called facing or lining board (Plate 258).

Facultative anaerobic: Organisms that grow either with or without oxygen.

Facultative parasites: Facultative parasites can adapt to live on dead organic matter and often have a wide host range and can easily be cultured on artificial media. These organisms may be aerobic or anaerobic but under changed conditions, they changes to anaerobic or aerobic.

Falling rate period: It is that part of the drying process during which the drying rate varies with time and the instantaneous drying rate per unit surface or weight of dry material continuously decreases. Falling rate takes place when most of the moisture has been evaporated during constant rate period. The falling rate period during dehydration take place in the final stage of dehydration when the food commodities have large amount of free water available for evaporation. Here, rate of removal of the moisture from the food to the surrounding is continuously decreasing / falling per unit time.

This chapter introduces the major biological, nutritional, environmental and management factors that determine egg quality. It examines genetic and breed influences, hen age and stage of lay, nutrition and feed composition, housing systems, environmental conditions and heat stress. The effects of disease, medication, handling, on-farm management and storage are also discussed. The chapter emphasizes that egg quality results from interactions among multiple factors rather than from a single intervention. Induced moulting and nutritional management are considered as practical approaches to maintaining quality during the laying cycle.

    1.    Wohler (1828) synthesized the first organic compound, urea from inorganic components.

    2.    Emil Fischer (1894) demonstrated the specificity of enzymes and the lock and key relationship between enzyme and substrate.

    3.    Michaelis and Menten (1913) developed kinetic theory of enzyme action

    4.    Watson and Crick (1953) Proposed the double-helical model for DNA structure

    5.    Ingo potrykus (1999) Golden rice- rich in b-carotene.

    6.    Glyoxysomes : Glyoxysomes are temporary in that they occur during transient periods in the life cycle of a plant such as in certain beans and nuts which store fats in their seeds as energy reserves. Glyoxysomes appear in the first few days after seed germination in endosperm cells and associate closely with lipid bodies. They disappear after the storage fats are broken down and converted into carbohydrate.

1. Lecithin is an example of
   1. Carbohydrates
   2. Hormones
   3. Vitamins
   4. Phospholipids
2. Which of the following acts as storehouse of energy in body?
   1. Hormone
   2. Vitamins
   3. Fats
   4. All of these

23.1 Introduction
Various products can also be produced by emulsions that contain fat globules. For instance, cream is produced when milk contains concentrated fat. This cream can be further processed to produce butter, ghee, and butteroil. “Milk fat, ghee, butter oil, anhydrous milk fat, and anhydrous butter oil are fatty products that are exclusively derived from milk or products obtained from milk, or both, through processes that result in the almost complete removal of water and milk solids, not fat.” The method of manufacturing (FSSAI standards) has notably influenced the flavour and physical structure of ghee.

23.2 Types of Fat Rich Dairy Products
23.2.1 Cream or Malayi
“Cream is a yellowish component of milk that naturally rises to the surface when milk is allowed to stand. It is rich in fat globules.” Cream is mechanically separated in the dairy industry. The size of the fat globules is diminished as a result of homogenisation of cream, which renders the product less suitable for whipping.
Cream can be defined as
1. That portion of milk, which is rich in milk fat
2. That portion of milk into which has been gathered and which contains large portion of milk fat
3. When milk fat is concentrated into a fraction of the original milk that portion is called cream.

Fat-rich dairy products such as cream, makhan, butter, butter oil and ghee have played a pivotal role in growth and development of dairy industry in India as well as abroad from the time immemorial. The reference of makhan and ghee has been found in old Indian scriptures. Makhan was popularized by Lord Krishna about 5000 years before. In the traditional Indian dietary regimen, milk fat in the form of malai (cream), makhan (freshly churned butter) contributes significantly towards nourishment of people of almost all ages.

Indian dairy industry, over the years, has been converting surplus milk for the manufacture of fat-rich dairy products, especially ghee and butter with skimmilk as a byproduct because of technological and economic reasons. More than one-third of the total milk production is being utilized for the production of ghee and butter wherein milk lipids, the most expensive constituents of milk are conserved and preserved. Milk lipids play many diverse roles, some of which are essential for human health. Many of the desirable flavour and textural attributes of dairy products are due to their lipid components, consequently, butterfat has, traditionally, been highly valued. Significance of fat can also be realized from the fact that the consumer’s perception of food quality is largely based on the perceivable rich taste. Unfortunately, milk lipids are subject to chemical and enzymatic alterations which can cause flavour defects referred to as oxidative and hydrolytic rancidity, respectively. The storage stability of high-fat products is strongly influenced by these changes.

Procedure: Measurement of smoke point of given oils: Heat the given oils in a deep frying pan till fumes come out from oil. Record the temperature when the fumes come out and noted it as a ‘smoke point’. Cool the oil by keeping oil pan at room temperature. After cooling repeat the heating of same oils 2nd and 3rd time to measure the smoke point and record the observations

Fats are obtained from both animal and vegetable sources. The principal animal fats are cream, butter, egg yolk, the fat of beef, mutton, pork, bone marrow, chicken fat, and codliver oil. Vegetable fats are those derived from fruits and seeds of plant, as olive oil, cottonsed oil, corn oil, peanut oil, soyabean oil and nut oils.

COMPOSITION AND NUTRITIVE VALUE OF FATS

Fats and oils, the most concentrated of fuel foods, were the first of the organic compounds or foods to have their composition determined. They are made up of same three elements which go to produce the carbohydrates, but the oxygen is less in proportion to the carbon. This explains the fact that fat yields more energy per gram than carbohydrates do.

Lipids are a class of compounds that are defined by how they are isolated, rather than by their composition. Lipids are molecules that can be extracted from plants and animals by low polarity solvents such as ether, chloroform, or even acetone. They are not appreciably soluble in water. Fats and the fatty acids from which they are made belong to this group as do oils, waxes and steroids. Hydrolyzable lipids are those that contain a functional group that will react with water. The functional group is usually an ester and the list of compounds includes neutral fats, waxes, phospholipids, and glycolipids. Non- hydrolyzable lipids lack such functional groups and include steroids and fat-soluble vitamins (e.g. A, D, E, and K).

Fats and oils are composed of triacylglycerols. These are compounds prepared by the union of glycerol (1,2, 3-tri- hydroxypropane) and 3 fatty acids to form a triester. Fats and oils belong to a group of biological substances called lipids. Lipids are biological chemicals that do not dissolve in water. They serve a variety of functions in organisms, such as regulatory messengers (hormones), structural components of membranes, and as energy storehouses. Fats and oils generally function in the latter capacity. Fats differ from oils only in that they are solid at room temperature, while oils are liquid. Fats and oils share a common molecular structure. The fats and oils contain three ester functional groups. Fats and oils are esters of the tri- alcohol, glycerol (or glycerine). Therefore, fats and oils are commonly called triglycerides, although a more accurate name is triacylglycerols. Triglyceride molecules contain mostly carbon and hydrogen atoms, with only six oxygen atoms per molecule. This means that fats and oils are highly reduced (un-oxidized). Oils are particularly common in seeds, where the stored energy helps seedlings during germination, until they can exploit solar energy through photosynthesis. Fatty acids contain an even number of carbon atoms, from 4 to 36, bonded in an unbranched chain. Most of the bonds between carbon atoms are single bonds. If all of these bonds are single bonds, the fatty acid is said to be saturated, because the number of atoms attached to each carbon atom is the maximum of four. If some of the bonds between carbon atoms are double bonds, then the fatty acid is unsaturated. When there is only one double bond, it is usually between the 9th and 10th carbon atom in the chain, where the carbon atom attached to the oxygen atoms is counted as the first carbon atom. If there is a second double bond, it usually occurs between the 12th and 13th carbon atoms, while a third is usually between the 15th and 16th.

Introduction India is a country of festivals and celebrations. Indian sweets enjoy a legendary reputation across the world. India is home to a number of delicious sweets that play a vital role in every Indian celebration. While gulab jamuns, rasgullas and kaju katlis may be the most popular sweets in the country (Pal Sanchari, 2016). Functions and events in India are celebrated with intake of sweets. Also, it is customary to sweeten the mouth in social gathering like marriage, birthday, poojas, etc. (Gulati and Misra, 2014). In the past few years, guidelines are formulated based on analyses of all published scientific studies on the consumption of sugars. In view of the rising prevalence of overweight, obesity and related disorders globally, in 2002, the WHO recommended that sugar should be less than 10 percent of daily calories. The consensus dietary guidelines for Indians in 2011 recommend less than 10% of total calories from free sugars per day. In May 2014, the expert panel of WHO recently recommended decreasing sugar intake to 5% of total calorie intake to combat obesity. A high intake of added sugars is a primary risk factor for diabetes. It is these added sugars in sweets that makes them palatable, but also does the physical damage. (Willet Brian, 2011). People have developed taste towards food in which fat and sugar contents are high.

Fermentation was known to mankind from time immemorial. But, development of biochemical principles of  fermentation  was originated by  Lavoisier in France in 1789 by way of analysing chemical composition of  sugar and its fermentation products such as ethanol, carbon dioxide and  acetic acid. Many products are produced by fermentation process. In fermentation, controlled action of selected microorganisms is used to alter  texture of foods, preserve foods by production of acids or alcohol or to produce fine flavours and aromas which increase value of raw materials.

Introduction

Fermentation is one of the oldest food preservation techniques that can be traced back thousands of years. Fermentation is referred as one of method of biopreservation as it leads to extending the shelf life of foods that involve the use of microorganisms and their metabolites. (Ross et al., 2002) For millennia, the fermentation of food by using micro organisms has been used as a technique for ensuring shelf life extension and improving the quality characteristics, texture and flavour of food products as well as to develop new innovative products. The major bacteria used in food fermentation worldwide are lactic acid bacteria (LAB). LAB is a multitude of genera including Lactobacillus, Lactococcus, Streptococcus, Leuconostoc, Pediococcus, and Enterococcus. Lactic acid bacteria constitutes a diverse group of bacteria that enjoys the status of “generally regarded as safe” (GRAS) and “qualified presumption of safety” (QPS) by the Food and Drug Administration (FDA) and European Food Safety Authority (EFSA), respectively. In this respect, starter microorganisms are known to produce a wide array of antimicrobial and protein-acoustic compounds to inhibit the growth of undesirable flora in foodstuffs resulting in shelf life extension. (Hill et al., 2017).

1. INTRODUCTION

Fermentation is an age old technology. It has been used by man since antiquity. It has been employed to produce a number of fermented products such as wine, beer, curd, vinegar, pickles and several others. Fermentation was discovered quite early and by chance but the underlying principles were discovered very late. Primarly fermentation was used as a method of shelf-life extension of the produce in the time of glut production, though the final product had modified sensory qualities. Thus, fermentation and drying were the main methods of preservation. With the discovery of new methods of preservation, fermentation as a method of preservation has gone into background and as technique of flavour development and product with modified sensory quality came into practice. In this chapter, fermentation as a method of preservation has been described.

Millets are highly nutritious and play a significant role in many people’s diets around the world. They have traditionally been transformed into a range of products through fermentation and malting for centuries. In the era of sustainable development, we still lag in using millets as a primary source of nutrition even after knowing that they are rich in terms of nutrition and other essential elements as compared to cereals. Today we have lots of information regarding different millets; nutrition content, methods of preservation, fermentation, malting, its products etc. Foods made from millet are regarded as potential prebiotics because they can improve the availability or functionality of probiotics, which has major positive effects on health. Foods have been fermented in order to enhance their flavor, texture and palatability. Although fermentation can result in a variety of products, the most prevalent ones are ethanol, lactic acid, carbon dioxide and hydrogen gas (H2 ). Commercial applications for these materials include use in food, vitamins, medications and industrial chemicals. Additionally, fermentation increases the amount of protein present, improves the accessibility of carbohydrates and reduces antinutritional components like tannin and phytic acid. Even though it has some drawbacks, such as the potential for contamination with dangerous microbes, fermentation technology is proven to enhance the nutritional quality of food products. This chapter covers the millet fermentation technology’s effects on the nutritional composition of the millets, contemporary developments in fermented millet-based products and technological improvements examined in the millet fermentation technology.

INTRODUCTION

For thousands of years fermentation processes were largely natural phenomena exclusively identified with food preservation throughout the world. Fermentations can play a significant role in meeting the ever growing food requirements of world population providing benefits in the form of innovative food products with improved nutritional value and food quality along side better safety. Fermentations can be used to address a large variety of food related problems and in the development of processes to meet the demand for food. In the wake of estimations made by international organizations that global food demand and population would increase by two-fold by the year 2050, much emphasis has been placed over the ways and means to increase food production and preservation.

Introduction

Wine is a very unique fermented beverage. It was an essential part of various religious and cultural ceremonies in ancient civilizations; and continues to be considered as a ‘drink for elite’. The reason is that wine was considered as ‘Gift of God and ‘God’s nectar’ in religious text books, and medicinal value of the beverage is well supported by modern science. Wine is a beverage well tagged with its geographical origin. This concept originally comes from French concept of terroir; which explains that a varietal wine from a defined region possess distinctive qualities. Wine makers of the past enjoyed this definition of quality for better marketing opportunities. Today, globalization and advancement in wine science created empowered consumers who could clearly discriminate quality of wines. In addition to a product with high sensory qualities, consumers expect a healthful beverage produced in an environmentally sustainable manner. Enology research projects focus on such aspects; and this chapter is a glance on enology advancements and proven health benefits of wine, such as cardiovascular health gut health, dental health etc.

Introduction

Fermented foods are those foods which have been subjected to the action of microorganisms or enzymes that cause desirable biochemical changes and significant modification of the food (Campbell-Piatt, 1994). Fermented foods are usually produced using ingredients of plants or animals in combination with bacteria or fungi. The bacteria or fungi are either sourced from cultures maintained by humans or from the environment. The origins of fermented foods go back many thousands of years, with early evidence of the alcoholic fermentations of barley to beer and grapes to wine. Evidence suggests that fermented foods were consumed 7,000 years ago in Babylon (Battcock and Azam-Ali, 1998).Throughout the world, the fermented foods are used by the people. It plays a major role in the diet in many developing countries. Several traditional fermented foods are prepared in rural areas of many countries.

Fermentation in food processing typically is the conversion of carbohydrates to alcohols and carbon dioxide or organic acids using yeasts, bacteria, or a combination thereof, under anaerobic conditions.

2.1. The Story of Fermentation

Human progress depends on man’s tact to tame the forces of nature. It calls for knowledge. Prior to Age of Science, our fund of such knowledge was gained by a process of trial, error and accidental discovery. Progress was due not so much to man’s understanding as it was to his unquenchable spirit, miraculous ingenuity and indomitable persistence.

At last, in the utmost urgency of struggle for a longer, healthier and more satisfying existence, humanity taught itself the scientific method, which has changed the world, and will change it more in the coming years. This chapter deals with one of the most momentous and far reaching of all scientific achievements: the ability to harness microscopic living creatures in the service of mankind. Thanks to the wonders of fermentation technology and biochemical engineering developed by scientists and skilled technicians in industrial, governmental and university laboratories around the world, billions of microbes are now at work in industry, producing substances that give us better food $#x0026; drink, better clothing $#x0026; shelter and better medicines. Thus, the human race has entered a new era of health, comfort and longevity.

In the Beginning Art without Science: The cave man discovered that meat allowed to stand a few days was more pleasing to the taste than meat eaten soon after the kill. The cave man’s earliest descendants learned that intoxicating drinks could be made from grains and fruits.

Introduction

Fermentation is an alluring procedure of biochemical adjustment of essential nourishment microorganisms and their enzymes accomplish by lattice. Fermentation is a utilized to upgrade bio-accessibility and bioavailability of supplements from various crops and improves organoleptic properties (e.g., appearance, generation of sour taste and new aroma compounds, and textural changes such as consistency or viscosity) just as increase the shelf life of realistic usability. It makes foodstuff safe not just hindering the growth of pathogenic microorganisms because of antimicrobial growth of lactic acid and it also detoxifies aflatoxin. With these advantages, fermentation is a successful method to reduce the risk of mineral deficiency among population, particularly in developing nations where unrefined cereal or pulses are profoundly consumed. Tragically, it is additionally connected with multiplication of microorganisms, for ex., yeast and molds caused food safety concerns, (Omemu et al; 2011), decrease in provitamin A and antioxidant (Ortiz et al; 2017), carotenoids, just as loss of nutrients and minerals (Hotz & Gibson, 2007).

Fermentation is a minimum cost-efficient procedure for production and preservation of nutrient, therefore fermented food is an important piece of the eating regimen in numerous of the world. In the nineteenth century the scientific study of microbiology began, and just because, the scientific procedure of fermentation was comprehended. several classifications have been utilized to sort the assorted exhibit of the fermented nourishment produced dependent on various microorganisms, and food types, and kinds of fermentation included.

The microorganisms associated with fermentation process fall into three classes: bacteria, yeast, and molds. Yeasts are the principal microorganisms for the fermentation of breads, whereas molds is mainly used in processing of cheese and legumes. Bacteria is included only of the fermentation of cereal product. cereal and tuber fermentation are a lactic and acetic acid producing bacteria. yeast is a piece of mix microbial fermentation by bacteria in weaning food; all the more frequently, be that as it may, alcoholic fermentation of yeast may not totally alluring. Four genera of lactic acid producing bacteria are recognized as overwhelming cereal fermentation to be specific, Lactobacillus, Leuconostoc, Pediococcus, and Lactococcus spp. all of which require carbohydrate for the energy. Lactic acid and other natural acids produced from hexoses bring down the pH from about 6.5 to about 3.6. In the event that the pH is beneath 4.0, fermented product retard the growth of pathogens and along these lines ensure fermented items.

Fermentation plays a major role in our diet and give the basic foods like bread, yoghurt, wine and cereal and legume based traditional fermented foods. The development of fermented food products can be considered as one of the greatest achievements of human civilization and goes back thousands of years. It arose from the necessity to conserve foods, to make them more digestible and also more enjoyable. During the centuries, man learned by trial and error to conduct the fermentation processes by changing physical and chemical parameters in the basic food ingredients. It took much longer, however, to discover the cause of fermentation processes: only towards the end of the 19th century it was demonstrated, that baker’s yeast is the cause of alcoholic fermentation. Through the development of the technique of "pure microbial culture", the basis for a controlled use of microbes in food industry was established.

The unique feature of fermented foods is the vital role of microorganisms that bring about essential bio-trasformations of the substrates during fermentation contributing number of desirable properties such as improved product shelf life, enriched diet with improved flavor and texture, increased safety, enriched nutritional supplement and probiotic functions. Fermentation is a chemical reaction carried out by many types of microorganisms to obtain energy. Fermentation is a process for production of a product by means of mass culture of microorganisms. In fermentation, microorganisms breakdown complex organic compounds into simpler substances. Although chemical changes and microbial growth usually mean food spoilage, in some cases fermentation is desirable and microorganisms are actually added to foods. For example, in the production of beer, wine, and other alcoholic beverages, yeasts convert sugar into ethyl alcohol and carbon dioxide. In the making of yogurt and cheese, lactic acid bacteria convert lactose, a sugar found in milk, to lactic acid. Alcohol, acids, and other compounds produced in fermentation act as preservatives, inhibiting further microbial growth. In addition to its use with alcoholic beverages, cheese, and yoghurt, fermentation is used to produce yeast bread, soy sauce, cucumber pickles, sauerkraut, and other traditional fermented food products. The variation in agroclimatic conditions prevailing in India and diverse form of dietary culture of various ethnical groups produced traditional fermented foods such as idli, dosa (rice-legume based), vada, papad, wari, balle (legume based), bhatura, jelabi, nan (wheat based), paneer, shrikhand (milk based).

Introduction

Eradication of malnutrition and hunger (Sustainable Development Goal, SDG-2) are the major challenges to humanity. Availability of sufficient nutritionally balanced food is the prerequisite for ensuring global food security. Food security is also critical for achievement of SDG 1 (poverty eradication) and SDG 3 (health and well-being) (UNDP, 2019). Changing climatic conditions accompanied by global warming not only acts a major threat for achievement of SDGs but could actually lead to the reversal of the progress already made. The escalating global temperature has jeopardized the food security throughout the world. A great decrease in the global crop yield has been suspected even with a 2°C rise in temperature. On field, it is often associated with crop nutrient loss, less accumulation of micronutrients and low availability of micro-minerals like iron and zinc. Additionally, the effect of the rainfall and temperature has also been observed. The global food prices are projected to increase by 3–84% by 2050 due to the fluctuations in rainfall and temperature (Hoegh-Guldberg et al., 2018).

Millets are the small seeded grains which belong to family Grassacae/Poaceae. Traditionally, millets are used for the food and feed purpose and are among the oldest cultivated crops. The millet species of commercial importance are proso (Panicum miliaceum), foxtail (Setaria italica), barnyard (Echinochloa esculenta), finger (Eleusine coracana), little (Panicum sumatrense) and pearl (Pennisetum glaucum) in different regions of the world. In India, foxtail millet (priyangava), barnyard millet (aanava) and black finger millet (shyaamaka) have been mentioned even in the Yajurveda texts of 4500 BC (Morya et al., 2017). The records of consumption of foxtail and proso millet are as old as 2000 to 1000 BC in China. Cultivation of foxtail millet in Korean peninsula is documented around 3,500–2,000 BC. Foxtail millet is the oldest of all the cultivated millets and is believed to be originated in Southern Asia. From there, it was spread to Europian nations. In the west, cultivation of millets majorly started after 1850 (ICRISAT, 2019)

Introduction

Fermentation of food dates back to ancient practice of wine making. Fermented foods reflect culture and tradition of folklore and emphasis their close association with nature. Various methods employed for fermenting foods aimed in food preservation to reduce wastage; however lactic acid fermentation enhanced nutrition content and improved organoleptic properties. Experimental evidences on benefits on health and wellness on consumption of fermented foods are being reported. Fermentation process used for preparation of indigenous or traditional foods often depend entirely on natural microflora on the surface of raw material and containers used; Practices of fermenting foods are passed on as traditional know how to generations as a legacy. The fermented food manufacturers rely on commercial starter cultures, and highly reliant on indigenous materials as microbial collections are unique and highly variable according to region and products. Therefore, isolation of novel organisms, development of newer products and interactions are being explored using variety of fermented foods obtained through traditional processes

Introduction

The art of fermentation process is developed as far back as thousand years ago without understanding the role of microorganism in it. It is believed that the process of fermentation originates in Indus valley during 7000 – 8000 BC. Archives of harappan civilization showed that different clay utensils were used for the fermented product and the agriculture and animal husbandry were fully developed during that period. It is evident from the artifacts of Egypt and middle east that the practice of fermentation was common in that part (Farnworth, 2003). The earliest practice of cheese making is originated from Falcate between Tigris and the Euphrates rivers in Iraq, 8000 years back. Later, Egyptians developed the use of yeast in wine and bread making during 4000 BC (Ross et al., 2002). Further, Sumarians prepared beer from fermented barley during 1750 BC. Earlier the fermenting process was an artisan way without any knowledge of microorganism. However, the main turning point was in 1608, when Antony Van Leeuwenhoek first established the use of a microscope and revealed the existence of microorganisms. Another, major finding was Pasteurization by Louis Pasteur in 1861 AD and this was the first report that documented the role of microorganism in fermentation. Later, there was a shift observed in the production of fermented food from local communities to large scale industry. The health effect of fermented food was first reported by Roman historian Pliny, who reported that fermented milk is beneficial for gastrointestinal complains. Further, a French paediatrician proved that Bifidobacteria in food prevents infants against infection (Grill et al., 1995).

1. Introduction

Now a days, foods are not intended to only satisfy hunger and to provide necessary nutrients but also to prevent nutrition-related diseases and improve physical and mental well-being of the consumers (Menrad, 2003). In this regard, functional foods play an important role. The concept of functional foods which has evolved as the role of food in the maintenance of health and well-being and in the prevention of disease, has received increased scientific and commercial interest is recent past. Growth of functional foods markets include increased consumer health awareness, increased popularity of healthy foods, search for food-based drug substitutes, self-medication approach promoted by busy lifestyles and rapidly developing health conscious markets. Functional foods can be defined as foods “that can beneficially affect one or more target functions in the body, beyond adequate nutritional effect, in a way relevant to an improved state of health and wellbeing and/or reduction of risk of disease” (Stanton et al., 2005).

14.1 Introduction
India is now the top producer of milk globally, accounting for 13.1% of the world’s total milk output. Fresh milk, if left at room temperature, will spoil within a few hours. To extend its shelf life, some of the fresh milk can be converted into fermented products. This fermentation process not only prolongs the product’s shelf life but also enhances its flavor and makes it easier to digest. Historical evidence suggests that people have been making fermented milk products since as far back as 10,000 B.C. Various strains of Lactobacilli have been cultivated in labs to create a diverse array of cultured milk products with unique flavors. Since as early as 10,000 B.C., milk has been utilized to produce fermented dairy items in different parts of the world. These fermented products offer benefits such as improved digestibility, distinctive tastes, added probiotics, vitamins, minerals, and extended shelf life. There is an enormous diversity of fermented dairy products globally, with each product’s characteristics influenced by the local strains used in the fermentation process.
14.2 Lassi
In India and Pakistan, lassi is a somewhat common beverage. It is produced by combining Indian spices with water or milk and curd or yogurt. Sweet lassi is made with sugar or fruits rather than spices; traditional lassi, sometimes called salted lassi, or just lassi, is a savory beverage occasionally seasoned with ground roasted cumin.

Introduction

Fresh fruit journey from the farm to the consumer’s table is an intricate process which needs a lot of attention. The way that the produce is harvested and handled is of supreme importance in relation to its quality shelf life and general market attractiveness. Improper handling practices during these critical stages can bring both quantitative and qualitative loses hence the perishable produce will lose its commercial value and appeal to the consumers (Kader, 2005). No doubt the significance of successful harvesting and post-harvest handling practices is crucial and has an impact on quality features and safety in case of fresh fruits. Poor management can cause physical assault ripening speed and more susceptibility to the growth of micro-organisms which makes the produce unsuitable for consumption or processing.

1. INTRODUCTION

Vegetables differ widely from field crops in their requirements for land preparation and cultural practices because of their differences in their morphologies, growth habit, and socio-economic values. In preparing land for vegetable production, ecological location, mode of cropping, season, crop disposition and the type of vegetables to be grown are taken into consideration.

Clearing: It is often necessary to remove the vegetation cover when a piece of land is to be used for vegetable production. The land clearing methods may involve removing the remains of previous crops and undergrowth, cutting back the woody shrubs and trees and burning them.

Levelling: When the site has been cleared, uneven land may have to be leveled. This facilitates the process of ploughing, harrowing ridging and layout of the site.

Land preparation and layout depend on

Ficus carica L, one of the oldest plant species in China, is abound in Shandong, Xinjiang, Jiangsu province. Fig belongs to the family Moraceae and genus Ficus. It is native to Middle East region (West Asia), but now it is widely distributed in Asia and North America.

Cultivars/Varieties: Depending upon the nature of flowers and the method of pollination there are four distinct classes of fig- i) Common fig or Adriatic fig ii) Capri fig, iii) Smyrna fig and iv) San Pedro fig. Kadota, Mission, Adriatic, Brown turkey, Celeste and Conadria are some important varieties of common fig.

Uses

Fig is consumed fresh or dried, preserved, candied or canned. Fresh figs are very delicious, wholesoms and nutritious and used as dessert or for making jam. Due to its highly perishable nature it is dried and marketed. The fruit fresh, or dried is valued for its laxative property. Dried and ground fig can be used as a substitute for coffee.

Filling converts freshly frozen ice cream into commercially marketable product formats while maintaining accurate portioning, appearance, hygiene, and structural quality. This chapter examines the major filling and forming systems used in industrial ice cream production. It covers cup, cone, moulded stick, extruded novelty, tub, and family-pack filling lines. The chapter explains equipment functions, filling principles, product handling, and operational considerations for different formats. Common filling defects and corrective measures are also discussed. Standard operating procedures for start-up, operation, and shutdown provide practical guidance for plant personnel. Effective filling operations are essential for achieving consistent weight, attractive presentation, packaging integrity, productivity, and efficient manufacturing performance.

a. Balance Sheets or Net worth Statement

Balance sheet is the list of assets and liabilities owned by a business. It helps to calculate the growth in net worth of the business by calculating the change in assets less the change in liabilities. They represent the financial foundation on which the business is built.

The term ‘balance sheet’ implies some sort of balance as part of the document. That balance represents the relationship between assets on one side, and liabilities on the other, and the basic accounting principle that assets always equal liabilities plus equity. Since it is simply a list of assets owned and debts owed, at a given time, with monetary values attached, we must understand the concepts of assets and liabilities.

Assets are resources owned by or owed to the business such as dairy, equipment, real estate, and notes receivable. Assets are those parts of the business that are owned or controlled. They include any property/resource owned by a person or business e.g. cash, land, buildings, dairy, farm equipment and dairy-related share. Assets are usually listed on the top or on the left hand side of the balance sheet. They are classified in following ways

A TTO needs a planned budget of five years to sustain it in the long term. This involves budgeting the infrastructure, manpower, research, and outreach programs. The budgetary needs of a TTO will be based on the size and scope of the TTO and the maturity of the parent institution it caters to. The initial years will be focused on capacity development, the appointment of experts, and the establishment of processes of intellectual property management. The mature TTO will generate income through the returns of the licensing agreements and the collaborative efforts with the industry. Nevertheless, external funding through grants and government schemes plays a vital part, particularly in the initial years. The planned budget ensures long-term stability and development of the TTO.
9.1 Budgetary heads
9.1.1 Operational expenditure
Operational expenditure refers to the ongoing costs incurred by a TTO to maintain its day-to-day operations. This includes the cost of maintaining the office, including rent, power and utility bills, computer and network infrastructure, patent management cost, and administration charges. The filing and legal charges also fall within this category. The overheads will be a heavy burden if not planned with finances properly. A TTO has to be provided with a properly planned operational budget that covers the daily operational expenses. The operational expenses help the office perform effectively, sustain the employees, and effectively manage IP. The operational expenses that the budget normally covers include:

    a.    General Principles and practices
    b.    Analyzing project appraisals and reports
    c.    Capital Expenditure decisions, reinvestment and payback
    d.    Assessing project profits.

a. Financing the dairy enterprise

Dairy rearing provides triple benefits of income, employment and nutritional security to the society and is important source of subsidiary income to small/marginal farmers and agricultural laborers. Finance is a prerequisite and of paramount importance for setting up of a dairy enterprise whether a dairy farming unit for milk production, sheep or goat unit/piggery unit, dairy/poultry processing unit etc. Decisions about credit are often most important judgments that people in the agricultural industry must make. These decisions often determine whether individuals operating in input, production, processing, and services based dairy enterprise would succeed in making profit.

1. Introduction Millets have gained popularity as staple foods worldwide, particularly in regions with hot and humid climates. These crops are often cultivated in marginal areas where major grains struggle to yield significant harvests (Thapliyal and Singh, 2015). One such millet is finger millet (Eleusine coracana), native to Ethiopia, and widely grown in Africa and India, where it is known by various names, including ragi and mandua (Table 1). In many developing countries, finger millet serves as a staple for a substantial portion of the population, especially those belonging to lower economic strata, providing essential nutrients (Chandra et al., 2016).

1.1 Introduction Millets were one of the first crops to be domesticated by mankind in Asia and Africa which later spread across the globe as critical food sources to the evolving civilizations. The earliest evidence of millets has been found in the Indus civilization around 3000 BC. Millets are a collective group of small-seeded annual grasses that are grown as grain crops, primarily on marginal land in dry areas of temperate, sub-tropical and tropical regions (FAO). However, due to the popularity of the cereal crops like wheat, rice and maize; millets have lost dominion and have become an underutilized crop in last few decades. Millets have the potential to be the crop of the future, as our major crops have reached yield plateau, also changing climate has introduced several new biotic and abiotic stresses to the major crops, so to feed the ever-growing population we need to shift towards other potential crops. India is the largest producer of millet as it contributes about 41% of the total millet production of the world (APEDA, 2020).

Introduction

Fermented meat and fish products have been consumed all over the world since times immemorial and represent one of the most important groups of food. Meat offers its own natural microflora. Hence a wide variety of products have been prepared since ancient times by varying the mixture of meat and salt as well as by the addition of spices and seasonings. Thus, fermented meat products characterize a diversity of flavors and textures and are receiving increased interest from consumers all over the world. Most of these products still depend mainly on local, traditional manufacturing processes since little scientific information is available; but scientific knowledge is very important for consistent production of high quality and safe products.

Meat is an essential part of non-vegetarian diet. It is liked for its unique taste and is a rich source of nutrients like good quality animal proteins, essential amino acids and fatty acids, minerals, trace elements and particularly B-complex vitamins (Singh et al., 2013). The growing concern of consumers regarding health and safety issues has led to the development of functional meat products that promote health and well-being, beyond their nutritional benefits (Grasso, et al., 2014; Tyopponen et al., 2003). This has driven researches in the meat processing sector to increase their efforts in producing such functional meat products, which have incorporated functional ingredients, reduced salt, nitrates/nitrites, cholesterol and fat and modified product fatty acid profiles (Zhang et al., 2010). Fermentation of meat produces a wide range of physical, biochemical and microbial changes in the muscle-based product

Takes clean, washed and edible fish-50-100 g for each method of cooking. And cook the fish according to the methods given table and record the characteristics of cooked fish.

Finfish and aquatic invertebrates are part of Fish Genetic Resources (FiGR) that has actual or potential value for culture and capture fisheries. Fish as a group, have the highest species diversity among all the vertebrate taxa. Out of 62,305 species of vertebrates recognized world over, 32,064 (~ 52%) are valid fish species; of which, the great majority (97%) are bony fishes, mainly teleosts; and the remaining (3%) are cartilaginous fishes (sharks & rays) and jawless fishes (lampreys and hagfishes) (Eschmeyer and Fricke, 2012). India is blessed with vast fishery resources, like exclusive economic zone (2.02 m sq. km), coast line (8,129 km), ponds and tanks (2.254 m ha), beels, ox-bow lakes and swamps (1.3 m ha), reservoirs (2.90 m ha), rivers and canals (173,287 km), brackish water (1.235 m ha) (Ayyappan et al., 2011). The country is fortunate to possess vast and varied fish genetic resources in different aquatic ecosystems and being home to 7.84% of global finfish diversity, with 3535 species of fish, of which 3035 are native species, representing 46 orders, 252 families and 1,018 genera. The 500 exotic fishes are also covered and majority of them are of ornamental fishes. Out of the 3035 native fishes, 1016 are freshwater, 113 are brackish water and 1906 are marine species and at least 258 species are of commercially important (NBFGR, 2016-17). India harbours a rich diversity of fishes and four out of 34 biodiversity hotspot areas of the world. The biodiversity hotspots i.e. the Western Ghats and North East India harbours maximum endemic freshwater fishes. The Western Ghats harboring about 306 finfishes out of which nearly 69% are endemic to the region (Gopalakrishnan and Ponniah, 2000). Likewise North East India, another biodiversity hotspot area, harbors about 240 fin fishes and nearly 40% are endemic to the region (Sarkar et al. 2012). In the past one and half decades more than 50 new species were described from these areas and more than 10 new species were described from the marine environment.

7.1 Introduction

Fisheries are emerging as one of the fastest growing food production system all-over the world. The potential for fisheries development world over and especially in India is highly promising in view of physical and biological resources available coupled with technological development. The demand for fish fishery products is ever increasing as the awareness of health benefits are being made available to public at large. The world fish production has not kept pace with demand which means less and less fish is available. Nevertheless, a large fraction of this valuable commodity is wasted due to deterioration after landing or consumer preference. An estimate by FAO [1] indicates nearly 20-25% of the total catches are wasted as postharvest loss. Better utilization of aquatic resources should therefore, aim primarily at reducing this enormous loss by improving preservation of fish and fishery products and upgrading the discarded low value fish to high value fishery products. The processing of fish into different types of products generates considerable waste. Commercially, fish is being processed into fillets, steaks, mince, canned products and a variety of ready-to-eat products. Fish processing plants contributes substantial wastes in the form of skins, bones, offal, exoskeletons from shrimps and nutrients in wash water from surimi plants.

Fish processing waste utilization is an important issue for the seafood industry both from a regulatory standpoint as well as one that has potential economic impacts. It is estimated that only 25-50% of the raw material is utilized for primary products (fillets, canned and stabilized mince production). The remaining 50-75% of the raw material is considered processing waste and is utilized for low-value products or disposed-off at sea. A large volume of solid waste is currently processed into fish meal, fish protein hydrolysates, and fertilizers. A small percentage is processed into higher valued items, such as, seafood flavourants, colourants, chitosan, enzymes, etc.

Introduction Aquaculture is one of the world’s fastest-growing food industries, providing a sustainable source of income for millions of people (Mohanty et al., 2018, Troell et al., 2014; FAO 2018). From the total world’s production around 33% of the all harvested fishes are not only utilized for human utilization for different reasons like size, species, quality, amount, non-accessibility of handling or transportation office. Today, waste generated from fish processing is a major source of pollution in the environment. Even when excellent grade fish is produced, only 50% of it is consumed by humans, with the remainder being discarded as waste. These wastes, which are dumped in an open environment without being treated, produces foul odour, which, as previously said, might be a reason of environmental pollution, water pollution and other municipal difficulties. Fish meal and oil made from low-grade fish and discarded fish parts not only solves many of these health and environmental issues, but also boosts the fishing and fish processing sectors’ overall revenue. With the rapid build-up in yearly extraction since 2000, global usage of natural resources has increased dramatically in recent years, reaching 92.1 billion tonnes and resulting in a 254% rise from 27 billion in the nineteenth century (Coppola et al., 2021). As a result, the research is stepping up its attempts to develop a circular bio-economy that aspires to increase the value of material flows while also achieving sustainable consumption and production. It is a new idea that is gaining traction for the goal of ensuring human quality of life through the effective and sustainable use of resources, energy, and infrastructure.