eChapters

Objective: Development of RTS beverage from seasonal fruits.
Material required
Fruit, juicer-mixer-grinder, preservatives, sugar, refractometer, pan and knife etc.
Principle
Ready To Serve(RTS): This a type of fruit beverage which contain at least 10% fruit juice and 10% total soluble solid besides about 0.3% acid. This is not diluted before serving.
Before undertaking the preparation of beverage, it is necessary to know techniques of extraction of pulp/juice from various fruits used for RTS.

Principle
Jelly: Jelly is a semi-solid product prepared by boiling a clear, strained solution of pectin containing fruit extract, free from pulp, after the addition of sugar and acid. A perfect jelly should be transparent, well set, but not too stiff, and should have the original flavour of the fruit. It should be of attractive colour and keep its shape when removed from the mould. Pectin (0.5-1.0%), acid (0.5-0.75%), sugar (65%), and water are the four essential ingredients. Pectin test and determination of end-point of jelly formation are very important for the quality of the jelly. Some recipes for jelly making are given below:

Principle
Jam: Jam is a product made by boiling the fruit pulp with a sufficient quantity of sugar to reasonable consistency, firm enough to hold fruit tissues in position. Apple, pear, sapota, apricot, carrot etc. are used for preparation of fruit jam. Jam contains 0.5-0.6 % acid and invert sugar should not be more than 40 %.
Total soluble solids (TSS)
The TSS value is defined as the amount of sugar and soluble minerals present in fruits and vegetables. A refractometer is based on the principle of total refraction. This is done by placing a few drops of distilled water on the prism in the specimen chamber of the back the cover. The distilled water reading should be zero. If it is not so, it should be set to zero with the scale correction knob. The specimen chamber is now cleaned with muslin cloth or tissue paper. For determining the TSS, a drop of sample (jam, juice and syrup, etc.) is placed on the prism and the percentage of dry substance in it read directly on scale in refractometer.

Principles
The quality of foods can be defined as the ‘composite’ of those ‘characteristics’ that differentiate individual unit of a product and have significance in determining the degree of acceptability of that unit by the consumer. The overall quality refers to technological, physiological, chemical, microbiological, nutritional; and sensory parameters to achieve the wholesome food. Sensory quality attributes such as taste, colour, texture, flavour and overall quality of any food products were evaluated by 9- point Hedonic rating test is used for the purpose of evaluation. This test measures the consumer’s acceptability.

Revolutionizing skin cancer detection
The diagnostic process for skin cancer is evolving. Physicians have in the past relied upon physical exams to be followed by scanning and biopsies taking anywhere between 15 days to return results. Although most abnormalities turn out to be non-cancerous, this is distressing to patients and contributes to healthcare costs. Additionally, certain growths that turn out to be cancerous remain undiagnosed since they have not been visible.
Breakthrough startup Damae Medical is set to reverse this process. The startup has developed an advanced probe that is able to take 3D images of the skin to unveil the full extent of abnormalities in real time. This enables doctors to diagnose in an instant, enabling improved personalized treatment. When an operation is required only diseased tissue is removed to prevent unnecessary treatments and improved outcomes in patients.
The Origins in the Institut d’Optique
The beginnings of Damae Medical can be traced to Palaiseau-based Institut d’Optique where in 2013 two photonics students envisaged potential in an emerging imaging tech. Together with the inventor himself in 2014, they cofounded Damae Medical by taking an exclusive license to the core family of patents held by three partner institutions. While this contract was beneficial in launching the company to an early growth spurt, in the long run created difficulty in exercising control. This was resolved by taking an equity-forcontrol purchase in 2019 by Damae to acquire the foundational patent

Technology transfer (TT) is entering a transformative phase shaped by globalization, digitization, and the growing role of start-ups in the innovation ecosystem. Traditional linear models of TT where inventions flowed from academic laboratories to industry through licensing are being replaced by dynamic, multi-directional, and collaborative frameworks. This chapter explores emerging trends that will define TT in the coming decades, including open innovation platforms, digital TT ecosystems powered by artificial intelligence (AI) and blockchain, the rise of entrepreneurial start-ups and spin-offs, internationalization of TT through cross-border collaborations, and sector-specific shifts driven by 5G, biotechnology, drones, and green energy. Drawing from global and Indian contexts, the chapter highlights how universities, governments, and industries can leverage these trends to build inclusive, resilient, and sustainable innovation ecosystems.
Three global shifts which will redefine TT
1. Digitization of knowledge: With AI, big data, and blockchain, knowledge and IP can be shared, tracked, and commercialized faster and more transparently.
2. Entrepreneurial ecosystems: Start-ups, incubators, and accelerators are replacing traditional firms as vehicles of commercialization, particularly in deep-tech and frontier technologies.
3. Globalization of innovation: Challenges such as pandemics, climate change, and food security require cross-border TT collaborations, making TT an instrument of both economic and geopolitical significance.

13.1 Challenges in the technology transfer process
Technology transfer (TT) is more complex and involves the complex interrelationships between universities, research institutes, the private sector, the state, and society. Though TT is all about translating research findings into purposeful solutions that can support the economy and society, it is not a linear process. The challenges can be described as legal, institutional, cultural, financial, policy, and human resource. All this is accompanied by bottlenecks that inhibit or decelerate the diffusion of promising technologies.
13.1.1 Legal and intellectual property challenges
The vital component of technology transfer is the proper management of intellectual property (IP), which is often the most challenging aspect.
• Ambiguities in IP ownership: During multi-institutional or shared funder research, ownership is quite controversial. As an example, when a government lab and a university collaborate on the project, there is ambiguity as to who would have the ultimate licensing authority over the technology: the university, the lab, or the funding agency. This kind of fight hinders commercialisation and scares off industry partners.
• Lengthy patent filing and prosecution timelines: In several jurisdictions, the filings, examination, and granting of a patent may take between three and five years or more. Such delay is not compatible with the rapid cycle of invention in other fields such as ICT or biotechnology, where patent claims may become invalid before they are granted.
• High costs of IP protection: International direct patenting, like under the Patent Cooperation Treaty (PCT), is costly and involves legal services, fees, and upkeep costs. These costs are excessively high, which cannot be easily compensated by universities and startups, and where technologies are not secured or limited to local markets.

Incentives are a powerful stimulus to transfer of technology. They can encourage researchers to get their results to market, a new career path for many of them. They can create a culture that attracts and retains entrepreneurial staff, scientists, students and technology transfer professionals (TTPs) that view the university as a career path and a vehicle for making a contribution to society. Working in a pro-entrepreneurship team of colleagues can also lead to getting people to think in terms of making a connection between their work and its application. Incentives can be short-term or long-term in nature, and can be tailored to individual or collective requirements. As it is likely to be challenging for universities to design incentives that cater to every researcher’s or TTP’s desire, they can design incentive programs to cater to various aspects of human. Nature.
12.1 Why incentives matter in this ecosystem
Incentives can be either financial and non-financial. These are needed to ensure that researchers and TTPs remain motivated, aligned with institutional goals, and committed to the long-term process of technology transfer and commercialization. Incentive programs serve several important purposes:
• Motivation: Incentives energize individuals to pursue innovation and commercialization, often over long- and uncertain-time horizons.
• Retention: By offering recognition, rewards, and career advancement opportunities, institutions can retain high-performing researchers and skilled TTPs.
• Alignment: Well-designed incentives ensure that the personal goals of stakeholders align with institutional and societal objectives.

A TTO fills the important intermediary position of linking research institution with industry so that innovations and new ideas can reach market. To be able to facilitate that effectively, the institutions must have well-defined R&D policies that include Sponsored R&D, Collaborative R&D, and Grant-in-Aid R&D. These ensure that the research remains properly managed, regulatory compliance assured and aligned with institutional priorities. This chapter has discussed about key policies that can help in successful operation of TTO while aligning institution’s mission and interests Various core policies that help in successful technology transfer include:
• Intellectual property policy
• Technology transfer policy
• Revenue sharing policy
• Startup/Spinout/Venture creation policy
• Conflict of interest/Conflict of commitment policy
Each policy has specific objectives. These policies collectively ensure a clear and consistent framework for technology commercialization.
11.1 Intellectual property policy
An IP policy is a formal document that provides guidelines for managing IP by an organisation. A strong IP policy build trust by ensuring efficiency and transparency. Other benefits of IP policy includes creation of environment to encourage innovation and commercialisation of IP. It also lays foundation to set out the rules for public-private partnerships while addressing issues among various stakeholders and ensures compliance. IP Policy is not law, but a proposed course of action.

Performance metrics and indicators
Traditionally, in the past, TTOs’ performance was measured in monetary terms, such as universities’ revenues derived through commercialization. The methodology, however, is prone to shortcomings, in light of the fact that various TTOs do not receive significant amounts of financial proceeds through their technology transfers. In addition, relying on income does not depict a TTO’s true potential, in light of the fact that it does not account for the total range of outputs of research and commercialization opportunities. Recognizing these limitations, academics have found innovative ways to measure TTO efficiency, such as monetary and non-monetary indicators. Financial indicators typically entail measurable outcomes such as number of signed licenses, income from licenses, royalties collected, equity income, and number of spin-offs or startups created. These are actual gauges of the economic returns from commercialization efforts, and in the majority of instances are used in the context of benchmarking the performance of a TTO in a comparative context, perhaps against peer institutions or industry averages. Non-financial indicators consist of royalty distributions to academics, having an integral role in encouraging academics to participate in commercialisation activity. By incorporating monetary and non-financial metrics, universities might have an enhanced, comprehensive evaluation system for their TTOs. Such evaluation is sure to ensure assessment extends beyond short-run monetary metrics and looks to incorporate several organization, institutional, and research-oriented determinants in driving successful technology transfer.

A TTO needs a planned budget of five years to sustain it in the long term. This involves budgeting the infrastructure, manpower, research, and outreach programs. The budgetary needs of a TTO will be based on the size and scope of the TTO and the maturity of the parent institution it caters to. The initial years will be focused on capacity development, the appointment of experts, and the establishment of processes of intellectual property management. The mature TTO will generate income through the returns of the licensing agreements and the collaborative efforts with the industry. Nevertheless, external funding through grants and government schemes plays a vital part, particularly in the initial years. The planned budget ensures long-term stability and development of the TTO.
9.1 Budgetary heads
9.1.1 Operational expenditure
Operational expenditure refers to the ongoing costs incurred by a TTO to maintain its day-to-day operations. This includes the cost of maintaining the office, including rent, power and utility bills, computer and network infrastructure, patent management cost, and administration charges. The filing and legal charges also fall within this category. The overheads will be a heavy burden if not planned with finances properly. A TTO has to be provided with a properly planned operational budget that covers the daily operational expenses. The operational expenses help the office perform effectively, sustain the employees, and effectively manage IP. The operational expenses that the budget normally covers include:

8.1 Key legal statutes and regulatory foundations
Various policy measures were also undertaken by the Government of India in due course of time. The Government of India enacted four science policies after gaining independence, i.e. Scientific Policy Resolution (1958), Technology Policy Statement (1983), Science and Technology Policy (2003) and Science Technology and Innovation Policy (2013). The 1983 Technology Policy Statement possessed a simple goal of creating indigenous technology and boosting efficient absorption and adaptation of imported technologies suited to the nation’s priorities and. The Technology Policy Statement of 1983 was followed up through the establishment of the Technology Information Forecasting and Assessment Council (TIFAC), an autonomous organization entirely owned by the Government of India under the Department of Science and Technology during 1988. TIFAC also offers some funding and supports development and commercialization of technologies through the home-grown technology scheme, among its other operations. The Technology Development Board Act (1995) was also a significant policy move of the government in the same direction. Also in recent times, the Government of India also unveiled its own National IPR Policy 2016 with the motto cum slogan “Creative India, Innovative India”. In addition, the policy is aligned to a number of other schemes of the Indian Government like “Make in India”, “Atal Innovation Mission”, “Start-Up India” and “Stand-Up India”, all aimed at encouraging creativity, innovation and entrepreneurship in the nation.

In today’s innovation driven economies, turning a great idea or new technology into something investors want to fund takes careful planning. This process is called investor readiness. which means making sure the idea works, has a market, and is presented in a way that makes investors feel confident. Therefore, Investor readiness means the preparedness of an innovation to attract and secure investment from potential investors. It has all the essential parameters in place that an investor would evaluate before deciding to invest. For inventors, this means showcasing innovation’s business potential, potential for growth, and the capability to provide a return on investment. Understanding investor readiness is important because it directly influences scalability and growth of invention. Even the most innovative ideas can struggle to find the financial benefits without showcasing business potential.
Why it is important to discuss investor readiness
Investor readiness is the state in which a technology, its value proposition, and its founding team are significantly prepared to engage with securing funding from potential investors. Without investor readiness, even high-potential innovations may fail to reach the market due to gaps in business clarity, legal assurance, or team maturity. TTOs play an important role in this process by acting as intermediaries between inventor and investors. Modern day TTOs are more inclined to prepare technologies and associated teams to attract investment. This expanded mandate of TTOs and brought in investor readiness. It requires a wellstructured approach focused on aligning academic innovations with investor expectations. TTOs help in creating this readiness by guiding researchers through commercial pathways, derisking technologies, and translating technical advancements into investment suitable.

Strategic partnerships and productive outreach play a crucial role for new technologies to successfully reach the market, drive growth, and strengthen a country’s global competitiveness, Outreach programs have various benefits, ranging from technology transfer awareness, communication of stakeholders, industry, government, to academy partnership promotions. These efforts help in establishing connections, communicating opportunities, and encouraging active engagement from key stakeholders. Outreach activities can be performed under two broad categories:
1. Written communication includes all communication which can be viewed, shared, or made accessible for a set of people in a passive manner. This includes reports, newsletters, guidance, and online content which provide insights of worth for stakeholders.
2. Human outreach includes all direct communication like c face-to-face or verbal form communication it included conferences, conventions, workshops, seminars and personal communication with industry leaders.
The type of outreach creates stronger, more personal connections. An effective outreach strategy usually combines both human and written outreach efforts. By following this strategy TTOs can expand and strengthen partnerships, and make it easier for research-led innovations to get transition from lab to market more effectively. This chapter explores key outreach models, strategic networking approaches, and proven practices for building impactful partnerships in technology transfer.

5.1 Technology readiness levels- Introduction
Technology readiness levels (TRLs) is measurement of the maturity level of a particular technology. These are based on a scale from 1 to 9, with 9 being the most mature technology. It is a map that shows whether a given technology is in a conceptual state or can be used in real-world applications and ensures innovations are thoroughly tested before being applied on a large scale. The TRL scale uses a set of questions designed to measure progress of a technology toward maturity. They help in reducing risks and improving efficiency. TRLs were initially designed in the 1970s for use by NASA to assess how prepared a technology was for space missions. Technology for space travel is always extremely sophisticated and with a high degree of risk. NASA required a system to assess systematically whether or not a new technology was ready to implement. By breaking down development into different levels, NASA was able to provide informed decisions on which innovations were ready to incorporate (figure 12). In due course, TRL proved to be so effective that other industries began to use it. Other entities like the European Space Agency (ESA), U.S. Department of Defense (DoD), BIRAC, DRDO and many other agencies have taken , to using TRL to guide R&D strategies. TRL helps in simplifying development, decreasing risks, and maximizing resource utilization.

4.1 Phases of technology transfer
To translate laboratory research into market ready product, it is important to understand steps or phases involved in this technology transfer process. The process typically includes six main phases, each with a different role (figure 9).
4.1.1 Research
Technology transfer begins with research, the critical stage of exploring and discovering novel knowledge, technologies, or innovations that may have potential commercial applications. The research phase can occur in academic institutions, research organizations, or private sector companies, often funded by public or private sources. Thorough research ensures that the technologies being transitioned are scientifically and technically robust, thereby increasing their potential for market success. Therefore, the research phase involves creating and evaluating new ideas, confirming their feasibility, and identifying IP prospects, all of which are key components of TT success.
4.1.2 Innovation disclosure
Innovation disclosure is the first formal step where the innovation is reported to the appropriate TTO or IP management team. This process ensures that the institution or organization recognizes the innovation and evaluates its potential for protection and commercialization. It includes following information:

The success of a TTO relies on a systematic approach encircling factors like assessment, valuation, marketing, negotiation, and post-transfer management (fi gure 8). This chapter covers the activities that are essential for the effective functioning of a TTO. Other activies Technology Assessement /Valuation Financial Managemnt Major Activities of technology transfer office Commercialization IP Management Fostering Innovation
Fig 8: Various activities of TTOs.
3.1 Technology assessment - A pillar of innovation commercialization
The process of transitioning an innovative technology to commercial reality requires a stringent and formal process known as technology assessment. This early stage is crucial as it determines the viability of an invention by analysing practicability of the invention as well as its uniqueness and the likelihood of it being adopted in the target markets. In the absence of this preliminary examination, the chance of technology’s potential being mismatched to the market can arise.
a) Scientific merit and technical feasibility
A key benchmark in assessing technologies involves the identification of
scientific validity and technical performance. An innovation’s viability depends on the fact that it complies with good scientific practice or the innovative application of a new paradigm that has empirically confirmable validity. It is important to determine whether the invention’s underlying mechanism can be reliably proven through established scientific practice. A working prototype allows for empirical testing in different conditions and serves as proof of practical application. In addition to that, scalability has to be tested critically. It often happens that innovations perform well in a controlled laboratory setting but lose efficiency or cost-effectiveness in the case of mass production. The viability of the implementation on a grand scale becomes a core concern in this phase of the evaluation.

The establishment of a technology transfer office is an important and complex process for an organization. It needs careful planning to ensure its long-term sustainability. Before establishing a TTO, it is essential to evaluate whether institution’s core mission includes research commercialization, and if the volume and quality of its research output justify the need for an inhouse TTO. For institutions that may not meet all criteria, those institutions can collaborate or outsource for TTO services. It is also important to decide about its operational scope, funding, management, and for what kind of issues the office should develop a policy. There is no “right” way to set up a TTO, but successful TTO does require considering some key issues. This chapter outlines key steps and considerations for establishing a TTO. It also explains what physical infrastructures are needed, manpower, outlines the responsibilities and powers of TTOs.
2.1 Evaluating the need for a TTO
Establishing a TTO is an important step for any research focused organization. But before initiating a planning process for a new TTO, the organization must review its relevance by considering a few fundamental points:
Key considerations for institutions
1. Is institution’s mission focused on “research commercialization” If an institute’s primary mission is education, or it does not support research it might not be justified to create a TTO. For such institutions, it usually makes better sense to seek alternative solutions to the occasional technology transfer without the expense of a formal office. But institutions with strong research commercialization focus, a TTO has the ability to contribute meaningfully to the mission. The Association of University Technology Managers (AUTM), USA has identified following key motivations for initiating academic technology transfer:

Introduction
Understanding the concepts of technology transfer, licensing, and commercialization is crucial as it plays an important role in understanding how scientific discoveries leads to generation of new technologies further making them the impactful solutions that benefit industries and society. In the context of Indian HEIs, this especially important because a large volume of impactful research often remains underutilized due to limited commercialization channels. This chapter explains the terminology, essential components of technology transfer, outlines its significance for the innovation ecosystem. It also outlines the evolution of India’s technology transfer ecosystem, and emphasizes while understanding its potential to boost India’s economy, encourage entrepreneurship, and strengthen global competitiveness. From initial idea to a market-ready product is complex and multiphased process. The stepwise process is shown in Figure 1. It highlights a structured pathway consisting of five core phases: Idea/Concept, Technology development, Intellectual Property (IP) rights, Technology transfer, and Technology commercialisation.
Phase 1: Idea/Concept stage
This process of technology transfer starts with the Idea/Concept phase, where an invention is created. Each phase plays an important role in transforming raw scientific knowledge into innovative solutions that benefit society and generate economic value. In this phase breakthrough ideas emerge, within labs. Researchers explore and develop initial proof-of-concept or prototypes to validate their ideas. At this stage, the focus is primarily on knowledge creation rather than commercialization. This pathway of technology transition is summarized in Figure 1.

Quality control plays a vital role in ensuring food safety and freshness. Inspecting, testing, and grading fruits and vegetables is essential to confirm that food products meet safety standards. In today’s globalized food industry, quality control tests are conducted at various stages throughout the supply chain, from cultivation and harvesting to processing, packaging, transportation, and distribution.
In agriculture, the quality of grain is determined by its intended use. In ethanol production, factors such as starch content are crucial, while in food and feed manufacturing, properties like protein, oil, and sugar are of primary importance. In the milling industry, the soundness of grain is the most significant factor in determining its quality. For grain farmers, high germination percentages and seed dormancy are essential characteristics to monitor. For consumers, visual qualities such as color and flavor are key indicators of quality. Quality control ensures that the grain meets the needs of both producers and consumers while maintaining food safety.
RESOURCES FOR QUALITY CONTROL
PRE-HARVEST TESTING
Pre-harvest tests are essential tools that help growers assess the needs of their crops. These tests assist in making informed decisions regarding nutrition or pesticide applications, determining the optimal harvest windows, and supporting various farm management decisions. By conducting pre-harvest testing, farmers can enhance crop health, improve yields, and ensure that their crops are ready for harvesting at the best possible time.
Starch Iodine Test
Starch is a carbohydrate present in various plants, including apples. As apples ripen, the starch gradually converts into sugar. The amount of starch in an apple

In India, the procurement and disposal of food grains are governed by several guidelines to ensure food security and fair market practices. Procurement is typically managed by government agencies like the Food Corporation of India (FCI), which purchases grains directly from farmers at minimum support prices (MSP) to stabilize market prices. Farmers are encouraged to sell their produce through designated government procurement centers, ensuring fair prices.
The quality of food grains is a key consideration in procurement. Grains are tested for moisture content, cleanliness, and pest infestation before being accepted. Once procured, the grains are stored in government warehouses, with regular inspections to prevent spoilage and pest damage. For disposal, food grains are distributed through public distribution systems (PDS) to ensure availability to consumers at subsidized rates. Any surplus grains may be sold in open markets, or occasionally exported, to maintain a balance in supply and demand. Effective management ensures food security and minimizes wastage.
PROCUREMENT POLICY OF WHEAT AND RICE
State Government agencies and the Food Corporation of India (FCI) are responsible for purchasing wheat and paddy within the designated period, ensuring that the products meet the prescribed Fair Average Quality (FAQ) specifications and are procured at the Minimum Support Price (MSP) for the Central Pool. This procurement supports the distribution of food under the National Food Security Act (NFSA) and other welfare schemes (OWS).
The Government of India (GoI), in consultation with State Governments and FCI, finalizes procurement estimates for wheat and paddy before each marketing season. These estimates are based on anticipated production, marketable surplus, and the agricultural crop pattern. The procurement of paddy and wheat is carried out under both the Decentralized Procurement System (DCP) and the Non-DCP system.

Inventory management involves tracking the stock levels of products, ensuring that there is neither overstocking nor understocking, and maintaining an optimal supply to meet demand. Proper inventory management minimizes wastage and helps in cost control.
Logistics management focuses on the movement, storage, and distribution of goods. It ensures that products are delivered to the right place at the right time, maintaining quality while optimizing transportation and storage costs. It includes planning for transportation routes, warehousing, and managing the flow of goods. Collateral management, particularly in agriculture and finance, involves managing assets (like stored grains) as collateral for loans or credit. It ensures that these assets are properly valued, secured, and monitored throughout the loan term. Efficient collateral management minimizes risks for lenders while ensuring that borrowers can access necessary financing for agricultural operations or business growth.
INVENTORY MANAGEMENT IN GRAIN STORAGE
In grain storage, “inventory management” refers to the methodical process of tracking and controlling both the quantity and quality of grain stored in facilities such as warehouses or silos. This involves ensuring optimal stock levels by overseeing incoming deliveries, coordinating outgoing shipments, and actively preventing losses due to pests, moisture, and spoilage, while upholding quality standards throughout the storage period.
Inventory management encompasses the ordering, storage, use, and sale of a company’s inventory, which includes managing raw materials, components, and finished goods, as well as their warehousing and processing. Industries like automotive and healthcare depend on efficient inventory management to streamline production and prevent delays. While inventory management was

The inspection of storage facilities and the food products they contain is crucial for preserving grain intended for human consumption. Stored grain is not simply a passive substance; it is a living, breathing organic entity that undergoes deterioration during storage. This deterioration can occur in terms of quantity, through dry matter loss (DML), or quality, with issues such as discoloration, mold contamination, sprouting, and caking caused by microorganisms, invertebrate pests (insects and arachnids), and vertebrate pests (rodents and birds). Additional weight loss happens during the handling, transportation, and processing of stored grains.
To gather accurate data on all forms of loss, regular and thorough inspection and sampling are essential. These inspections are crucial for making informed management decisions regarding remedial actions against biological deterioration agents or the disposal of grain, taking into account the condition of both the commodity and the storage environment. Regular inspections help maintain a storage environment that supports grain quality by keeping track of any significant pest population increases, grain temperature, moisture migration, spillage, and grain residues.
Such inspections also encourage the general upkeep of the storage structure and its surroundings, which helps to minimize pest access. By addressing cracks and crevices in the storage fabric and removing old, often infested, bags and equipment, the risk of pest infestation is significantly reduced.
The inspection should address several factors:
• The presence of pests, if any
• Identification of species, population densities, and locations
• Damage inflicted prior to inspection
• The growth potential of pest populations or whether they will die out if conditions remain the same

Storage plays a crucial role between harvesting and the processing or direct consumption of food grains. In developing countries, its significance is amplified, especially since small and medium landholding farmers heavily rely on on-farm storage. In India, however, an annual storage loss of 14 million tons, worth approximately 7,000 crores, occurs due to post-harvest pest damage. The economic loss from storage pests is not only a result of consumption but also due to contamination and spoilage caused by the pests’ body parts, excreta, and hoarding. The lack of awareness about storage pests, their damage, and the use of improper storage techniques contribute to these massive losses.
The solution lies in constructing suitable storage structures, which are wellequipped and properly managed, and implementing strict hygiene standards in storage areas, along with efficient pest control measures. These combined efforts are essential to minimize storage losses. This section focuses on identifying major pests in storage and their management strategies.
SIGNIFICANT INSECT PESTS OF GRAINS THAT ARE STORED
The primary insect pests that affect stored grains include:
• Lesser grain borer (Rhizopertha dominica)
• Rice weevil (Sitophilus oryzae)
• Khapra beetle (Trogoderma granarium)
• Rust red flour beetle (Tribolium castaneum)
• Long headed flour beetle (Latheticus oryzae)
• Saw toothed beetle (Oryzaephilus surinamensis)
• Rice moth (Corcyra cephalonica)
• Almond moth (Cadra cautella)
• Angoumois grain moth (Sitotroga cerealella)

The design and structure of a warehouse should facilitate proper ventilation, temperature control, and protection from pests. This includes features like smooth floors, durable walls, and proper drainage systems to prevent water accumulation. The warehouse should also be insulated to protect stored goods from extreme temperatures and humidity fluctuations.
Regular maintenance is essential to keep the warehouse in good condition. This involves routine checks for structural integrity, such as inspecting for cracks, leaks, or signs of pest infestation. Equipment like ventilation fans, conveyors, and lighting should be regularly serviced to ensure optimal functioning.
Sanitation and hygiene are equally important in preventing contamination and pest issues. The warehouse should be thoroughly cleaned to remove any dust, dirt, or residues that could harbor pests or mold. All surfaces, including walls, floors, and storage bins, should be disinfected regularly. Proper waste disposal systems should be in place, and areas where food products are stored should be kept free from garbage or organic waste that could attract pests. Ensuring a clean and well-maintained warehouse helps preserve the quality and safety of stored goods.
SELECTION OF STORAGE TYPE
Some basic Guildelins or Principles are Given as Follows:
ROUND OR RECTANGULAR STORAGE
In terms of structural cost per tonne of storage, round storage buildings are generally more cost-effective than rectangular ones. The reasons for this are straightforward:

The quantity of space available for product storage is referred to as warehouse capacity. Understanding the distinction between theoretical working capacity and storage capacity can be aided by warehouse capacity. In warehouses and other storage facilities, space is perhaps the most valuable resource for online retailers. By making the most of their warehouse space, businesses can significantly increase the quantity of goods they can store there. Having more things in storage at once can save them money on transportation and other expenses.
It also makes it easier for the warehouse KPI staff to access the products, which helps them process orders faster and deliver the goods to customers sooner. Because space can be limited in a warehouse setting, warehouse managers and business owners should take the time to assess how much space they have and how well they’re using it.
STEP TO CALCULATE WAREHOUSE STORAGE CAPACITY
Understanding your warehouse’s current capacity is the first step in
determining how to increase it. This brief guide will show you how to calculate a
warehouse’s capacity in feet:
• Calculate the total square footage of your warehouse complex. Let’s assume that it is 10,000 square feet in this case.
• Determine the total amount of space devoted to non-storage purposes, such as offices, loading areas, restrooms, and break rooms. Assume for the moment that this is equivalent to 2,000 square feet.
• Subtract the total square feet of non-storage space from the total square feet of your warehouse to determine the usable space. The following is the equation:
USABLE SPACE-TOTAL SQUARE FEET – NON-STORAGE SPACE SQUARE FEET
Determine the total usable storage space by identifying the maximum stack height or clear height. This should account for the lowest-hanging overhead

Cleaning, drying, and aeration are essential processes in the management of stored agricultural products to maintain their quality and reduce the risk of spoilage. These practices are particularly important in preventing the growth of molds, the infestation of pests, and the deterioration of the product during storage.
Cleaning is the first step in the storage process. It involves removing any debris, dust, dirt, and residues from both the stored products and storage facilities. Proper cleaning eliminates potential sources of contamination, such as pests, molds, and bacteria, which can thrive in the presence of organic matter. It also ensures that the grain or other stored product is free from contaminants that could affect its quality and marketability.
Drying is equally crucial to prevent moisture-related issues. Excess moisture can lead to mold growth, fungal infections, and insect infestations. Drying reduces the moisture content of the grain or product to safe levels, typically below 14% for grains, which helps preserve their quality during storage.
The drying process can be carried out using natural sunlight or mechanical dryers, depending on the volume of the product and available resources. Properly dried products are less prone to spoilage and can be stored for longer periods. Aeration involves the circulation of air through stored products to maintain optimal temperature and humidity levels. By controlling the environment, aeration helps prevent the buildup of heat and moisture, both of which can lead to product deterioration.
It also reduces the risk of insect infestations by keeping the grain cool and dry. Aeration systems typically use fans or ventilation channels to ensure continuous airflow, especially in large-scale storage facilities. Together, these processes form a comprehensive approach to maintaining the quality and safety of stored agricultural products.

Warehousing is a critical national strategy for conserving food grains. The proper storage and maintenance of notified commodities, primarily food grains, alongside safeguarding them from adverse weather, rodents, and natural decay, are vital for fostering the growth and development of the agricultural sector.
Post-harvest losses contribute to approximately 10% of total food grains, primarily due to improper storage methods, insects, rodents, microorganisms, and birds. The Punjab State Warehousing Corporation (PSWC) takes on the responsibility of storing, handling, and warehousing notified commodities, including agricultural produce, industrial raw materials, finished goods, and various perishable and hygroscopic items. PSWC provides scientifically designed warehouses that protect both the quality and quantity of stored goods, aided by trained personnel.
At PSWC warehouses, stored products are safeguarded from rodents, pests, and moisture, ensuring their preservation. The provision of scientifically managed storage facilities for food grains, from procurement to distribution, is a vital function carried out by PSWC. PSWC has made significant strides in creating warehousing facilities throughout the state, constructing numerous warehouses in key rural and urban areas.
Rail-side warehousing facilities have also been established, offering costeffective and efficient storage solutions under a unified approach. Additionally, PSWC helps prevent distress sales, enabling producers to hold their products until favorable market conditions arise, thereby securing the best possible value for their goods.
Horizontal sheds are commonly used to offer low-cost, large-volume storage, and the Food Corporation of India (FCI) has also built very large sheds for storing grains and other products. These sheds typically feature steel or corrugated sheet

Agricultural professionals continuously confront challenges in preventing pest infestations and managing temperature and moisture levels to protect grains from decay. They recognize the critical importance of safeguarding these valuable resources and rely on quick, dependable solutions to tackle common grain storage issues. Let’s take a closer look at the challenges faced by growers, handlers, transporters, and processors in long-term grain storage, and the strategies they employ to overcome these obstacles.
ABIOTIC FACTORS
There are also abiotic factors, which are non-living elements, that influence the quality of stored grains. Factors such as air, humidity, and temperature play a significant role in grain storage. If these factors are imbalanced in terms of quantity or quality, they can have a detrimental effect on the stored grains.
SPACE CONSTRAINTS
As crop yields continue to rise, farmers face the challenge of finding enough storage space to accommodate the increased harvest. Often, last year’s grain still fills storage bins when the new crop arrives, prompting the agricultural industry to seek innovative storage solutions.
The most straightforward solution to a shortage of storage space is to build additional bins. However, this option is not always feasible for smaller farms or processing facilities with limited budgets. As a result, many choose to repurpose existing barns or store surplus grain outdoors in piles. Unfortunately, this outdoor storage method comes with its own set of challenges, including faster spoilage and pest issues. To counter these problems, some facilities use temporary storage options such as grain bagging systems or outdoor storage tents. Mechanical ventilation systems are also employed to ensure proper aeration, reducing the risk of spoilage before the grain is moved to its final destination.

Food is a vital requirement for all living beings, as it provides the essential nutrients necessary for growth, development, and recovery. Humans depend on both plants and animals for nourishment, which we acquire through agricultural practices and animal farming. With the increasing global demand for food, crop yields have risen, which has led to a greater reliance on warehouses for grain storage. However, merely increasing food production is insufficient to combat hunger and malnutrition, as food security also heavily depends on ensuring that food is accessible to everyone. Crops, however, are exposed to various threats, such as pests, weeds, and diseases, which can drastically reduce yields if not managed adequately.
A significant issue is storage losses, which are driven by both biological elements (such as fungi, insects, and rodents) and environmental factors (such as unsuitable storage conditions or extreme temperatures). These losses result in various negative consequences, including:
• Reduced food quality
• Lower seed germination rates
• Loss of weight in crops
• Discolored produce
These problems can impede the marketing of harvested crops, leading to unsold goods and considerable losses for farmers and distributors.
To reduce these losses, it’s crucial to adopt proper measures like efficient treatments and continuous monitoring of storage conditions. Grains should be stored in environments that prevent deterioration. This can be achieved by ensuring thorough cleaning before storage, drying grains thoroughly (both under the sun and in the shade), and taking steps to control pests, including fumigation.

The number of hormones secreted by the body exhibits control over the physiological regulation of glucose sugar level (homeostasis) in the manner needed by the body. Depending upon the situation, hormones may increase or decrease the level of glucose sugar present in the blood or tissues. The glucose is oxidized to yield energy, carbon dioxide, and water.
Glucose, more than the body’s needs, is stored or converted mainly to glycogen and, to some extent, protein and fat. If glucose is less than the need of the body, its denovo synthesis takes place from amino acids or fatty acids, or is formed from the breakdown of stored glycogen. Various hormones are secreted by multiple endocrine glands to control the blood glucose level. If it fails, it leads to hypoglycemia or hyperglycemia conditions. Gluconeogenesis is not developed at birth in the case of a baby pig. Therefore, hypoglycemia in baby pigs is observed within 2 days of age if starved, leading to hypoglycemia, weakness, confusion, and death.
15.1 The Hormones Involved in Homeostatic Regulation of Blood
Glucose Level
The hormones influencing carbohydrate metabolism are explained below:

Carbohydrates (glucose) are the primary energy source for a body; when digested, glucose is the end product in human beings and simple stomach animals, but it yields volatile fatty acids in ruminants. Precise regulation of levels of blood sugars/glucose in the body is the net result of various processes like digestion, absorption, and metabolism operating under the interaction of various a) enzymes and b) hormones secreted by the different organs of the body. An abnormal secretion of any of the enzymes or hormones associated with the metabolism of carbohydrates disrupts the precise regulation of blood sugar, leading to various disorders.
14.1 Enzyme Deficiency and Disorders in Carbohydrate Metabolism
Usually, various enzymes are involved in the breakdown of carbohydrates, and if their secretion is insufficient, missing, or does not work correctly, it leads to different disorders.
I. Glycogen storage diseases (GSDs, glycogenosis, and dextrinosis)
Glucose is needed by the cells for the generation of energy and other cellular activities. If glucose happens to be in excess, it is stored as glycogen, mainly in the liver and muscles, for future needs. The stored glycogen can be converted back to glucose when the need arises. Many enzymes are related to this interconversion of glucose and glycogen. Deficiencies of one or more enzymes may lead to abnormal glycogen metabolism, leading to glycogen accumulation of normal or abnormal chemical structure in tissue, collectively called glycogen storage diseases (GSDs). Roman numerals identify different types of glycogen storage conditions. They are also classified under various categories like inherited metabolic diseases, autosomal recessive type, and X-linked type (VIII/IX type).

Glycogen breakdown is known as glycogenolysis. This breakdown occurs in the cytoplasm of cells of the organs, wherein glycogen is stored. The end product of glycolysis is glucose in the liver, which is glucose-1-phosphate in skeletal muscles.
Glycogen is a polysaccharide synthesized from glucose monomers. Glycogen is stored in the cell instead of glucose because glucose requires more water, whereas glycogen can be stored with much less water. Most of the stored glycogen is in the liver (5% of body weight) and muscle (1 to 2 % of body weight). Muscle and liver glycogen undergo glycolysis for different purposes. Muscle glycogen provides energy for muscular activity, whereas liver glycogen helps to maintain the blood glucose level.
Glycogen contains many glucose residues (up to 120,000) linked by alpha 1-4 glycosidic bonds, forming a straight chain. Glycogen has branches at alpha 1-6 glycosidic bonds at every ten glucose residues. At the reducing end, the carbon one of glucose is free, whereas it is not accessible in the case of non-reducing ends.

Glycogenesis helps to maintain blood glucose, as it is a primary source of energy for every cell. An inadequate supply of glucose to the body may lead to the failure of the vital organs. Glucose, when in excess, is synthesized into glycogen and stored (glycogenesis), mainly in the liver and muscles (including the uterus). It is also found in other tissues, such as the kidney, brain, heart, and adipose tissues. The stored liver glycogen is broken down (glycogenolysis) into blood glucose when needed. Still, the glycogen stored in muscle is broken down to glucose 1-phosphate and converted to blood glucose later. Either the synthesis or breakdown pathway is primarily active at a time in tissues. Glycogen synthesis and its breakdown are not reversible processes.
The percentage of glycogen stored in skeletal muscles is less ( 1% to 2%) than in the liver (10%). Still, the total quantity of glycogen is greater in muscles due to their greater mass than an animal’s total liver mass. Muscle mass, on average, ranges from 40 to 45 % of body weight for the most mature mammals.
Glycogen synthesis (glycogenesis) is an anabolic process forming a branched homopolysaccharide made of several chains of alpha D-glucose units linked by α-1,4 glycosidic bonds in a linear chain and nearly every ten glucose residues; the chain branches off via α-1,6 glycosidic linkages. The α-glycosidic bonds give rise to a helical polymer structure. The number of glucose units in a single glycogen molecule varies (1,700 to -600,000 units). The glycogen granules are formed on the glycogenin protein in the center, so it acts as a primer, and their number determines the total number of glycogen particles.

Plants, with the help of photosynthesis, convert simple molecules (like carbon dioxide and water) into more complex molecules (anabolism) through various chemical reactions. Animals synthesize more complex molecules (anabolism) from monomer units through multiple chemical reactions. Anabolism refers to a biosynthesis process involving the sequences of biochemical reactions by which complex molecules are formed in living cells from available monomer units. Anabolic processes include the biosynthesis of complex biomolecules like carbohydrates, proteins, and lipids. The anabolic processes require energy from high-energy bond compounds like ATP.
The anabolic processes related to the biosynthesis of carbohydrates include
(1) Gluconeogenesis and
(2) Glycogenesis
11.1 Gluconeogenesis
Gluconeogenesis is the synthesis of new glucose molecules from noncarbohydrate molecules. The non-carbohydrate molecules that are used for gluconeogenesis are derived from glycolysis, lipolysis, and proteolysis. Examples are pyruvate, lactate, glycerol, and glucogenic amino acids. The liver and kidneys are the preferred organs for the gluconeogenesis process. It also occurs to some extent in the brain. Blood glucose levels are maintained at physiological concentrations through the process of gluconeogenesis in humans and ruminants. The blood glucose drops below normal levels during various conditions like fasting, starvation, pregnancy, lactation, fever, and low carbohydrate diets.

The uronic acid pathway is a minor and alternative pathway for glucose oxidation and for the production of uronic acid sugar. Uronic acids of sugars are formed when the terminal carbon’s (C6) hydroxyl group (CH2OH) of sugar gets oxidized to a carboxylic acid. Uronic acid names depend on the name of the parent sugars. Uronic acid of glucose is glucuronic acid, hexose uronic acids are hexuronic acids, and pentose uronic acids are named penturonic acids. The uronic acid pathway is located in the cytosol of the liver and adipose tissue.
The glucuronic acid should not be confused with gluconic acid (aldonic acid) and glucaric acid (aldaric acid or dicarboxylic acid). Gluconic acid (aldonic acid or “onic” acid) is obtained from D-glucose by oxidizing its aldehyde group at C1 to a carboxyl group. Glucaric acid (aldaric or dicarboxylic acid) is formed upon oxidation of both the terminal hydroxyl groups (C1 and C6) and the aldehyde. They have two carboxylic acid groups. This pathway yields pentoses & ascorbic acid or vitamin C (not in primates & guinea pigs) from glucose.

This alternative pathway for glucose oxidation has enzymes in the cytosol through which glucose is degraded to carbon dioxide. It is also called a shunt because carbon atoms from glucose 6-phosphate take a long route before they proceed down the EMP or glycolytic pathway. This pathway is anabolic in nature as it produces many intermediates. In this pathway, two NADPH are generated from one glucose 6-phosphate molecule. No ATP is generated or consumed.
Overall, this pathway needs three glucose-6-phosphate molecules to complete all the reactions. Therefore, one cycle run produces 2 mol of fructose-6- phosphate, 1 mol of glyceraldehyde-3-phosphate, and 3 mol of carbon dioxide from 3 mol of glucose-6-phosphate with the reduction of 6 mol of NADP+ to NADPH.
Overall reactions of the HMP pathway are as follows:
3 Glucose-6-P + 6 NADP+→ 3 ribulose-5-P + 3 CO2 + 6 NADPH
3 Ribulose-5-P → 2 xylulose-5-P + Ribose-5-P
2 Xylulose-5-P + Ribose-5-P → 2 fructose-6-P + Glyceraldehyde-3-P

The Hans Krebs cycle, known by different names like the citric acid or tricarboxylic acid cycle, was named after the scientist Hans Krebs. The citric acid cycle is named after the citrate product formed in the cycle. The name tricarboxylic acid is given as citrate itself, which is a tricarboxylic acid due to the presence of three carboxyl functional groups (-COOH).
The tricarboxylic acid (TCA) cycle occurs in aerobic conditions and within mitochondrial ETC, involving various sequential oxidoreductase reactions. The cycle requires Acetyl-CoA (C2 molecule) and oxaloacetate molecule (C4 molecule) to begin with the formation of a citrate molecule (C6 molecule). Acetyl-CoA is generated from the pyruvate (glycolysis), the fatty acids (lipolysis), or by oxidative deamination of specific amino acids. The complete oxidation of a glucose molecule results in the end products like energy (ATP), CO2 and H2O. A molecule of Acetyl-CoA has two carbons. It is formed from a three-carbon pyruvic acid molecule generated from the glycolysis of a sixcarbon glucose molecule. Acetyl-CoA is the substrate for this cycle and is an energy-rich molecule having a thioester bond between coenzyme A and acetic acid.
8.1 Formation of acetyl-CoA from Pyruvic Acid
Pyruvate is obtained by glycolysis or the oxidation of glucogenic amino acids. The pyruvic acid produced in the cytoplasm crosses the inner membrane of the mitochondrion to enter the mitochondrial matrix, where it undergoes oxidative decarboxylation, forming Acetyl-CoA. Acetyl-CoA carries (carrier molecule) the acetyl group to the citric acid cycle. It also links various metabolic products with the TCA cycle. Such products can be from the EMP cycle, beta-oxidation, and oxidative deamination of amino acids. Oxidative decarboxylation of pyruvic acid needs a complex enzyme system known as pyruvate dehydrogenase

Glycolysis involves a series of reactions to extract energy from glucose to
release two molecules with three carbons called pyruvate. The process is not
dependent on oxygen but can occur under aerobic and anaerobic conditions,
releasing different end products. Under aerobic conditions, the pyruvate formed
is then oxidized in the mitochondria to CO2 and H2O. The energy released in
glycolysis is in the form of ATP (High Energy Bond). All the reaction steps
occur in the cytoplasm, as it is the extramitochondrial pathway. In anaerobic
conditions and the absence of mitochondria (RBC), the end product of
glycolysis is lactic acid.
The net reaction of Glycolysis (anaerobic) may be summarized as under
Glucose + 2ADP + 2Pi → 2 Lactate + 2ATP
7.1 Steps in EMP Cycle or Glycolytic Pathway
Table 7.1: Steps and enzymes of glycolysis or EMP cycle.

6.1 Metabolism
Dietary components like carbohydrates, lipids, and proteins are continuously broken down and synthesized through various chemical reactions (exergonic or endergonic) that are concerned with energy. These processes not only break them down but also interconvert them for their synthesis. Dietary components, when utilized, are known as nutrients the body. The number of reactions occurring in living cells maintains the body cells and fluid’s homeostasis and dynamic status. To keep the dynamic state of a cell, many reactions are going on to synthesize or break down the different types and sizes of molecules. The total of these reactions is known as metabolism.
The metabolism may be anabolic or catabolic. Further, it may be aerobic or anaerobic, depending on oxygen availability. These four terms are explained below:
I. Aerobic and Anaerobic Metabolism
• Aerobic metabolism-- In aerobic metabolism, the carbohydrates (substrate) in the presence of oxygen are metabolized to release energy, carbon dioxide, and water. The released energy is converted to ATP by two processes in the mitochondria (the Krebs cycle and oxidative phosphorylation). In oxidation, the electrons released from a glucose molecule are transferred to reduced NAD+ and FAD. The oxygen (electron acceptor) is reduced to water. Water formation [from H2(g) and O2(g)] is an exothermic reaction. The energy released from the chemical bond of glucose is captured as ATP, and heat is released.

Polysaccharides comprise several monosaccharide units (more than ten) linked by glycosidic bonds; therefore, they are high molecular weight carbohydrates. They are also called polyanhydrides of simple sugars. The most common monosaccharide unit of polysaccharides is D-glucose. The polysaccharides may differ in their monosaccharide units, molecular weight, chain nature, glycosidic bond types (alpha, beta), and linkages (1-2, 1-3, 1-4, or 1-6). They are tasteless, colorless, amorphous powders that are a little water-soluble. Some may form colloidal solutions. The polysaccharides provide structural stability (structural polysaccharides) to cells, prevent cells and tissues from drying out due to their water-binding properties, and reserve food for the future. Reserved polymeric carbohydrates are osmotically less active and can be stored in large quantities within the cell.
The right and left end of polysaccharides, respectively, are called the reducing and non-reducing ends. Based on the functional aspects, they are grouped in two ways.
• The first way depends on its digestibility—1. Nutrient polysaccharides are (digestible) starch, dextrin, and glycogen, and 2. Structural polysaccharides are (indigestible), chitin and cellulose.
• The second way, depending upon the constituent monosaccharide units, they are 1. Homoglycans (same units), and 2. Heteroglycans (different units).

Oligosaccharides comprise 2 to 10 units of monosaccharide molecules, which are released upon hydrolysis. The oligosaccharides are classified depending upon the number of monosaccharide units, e.g., Disaccharides (two monosaccharide units), Trisaccharides (three monosaccharide units), Tetrasaccharides (four monosaccharide units). Disaccharides are the most common oligosaccharides found in nature.
4.1 Disaccharides
Disaccharides are formed when glycosidic linkages covalently bond two monosaccharides together. A glycosidic bond is formed when the hydroxyl group on one of the sugars reacts with the monomeric carbon on the second sugar. A glycosidic bond can be alpha or beta, depending on the steric configuration at carbon one on the monosaccharide unit. During the union of two monosaccharide units, water molecules are eliminated (dehydration reaction or condensation reaction, or dehydration synthesis), and the formation of a glycosidic bond occurs, through which the units are linked via an oxygen bridge.

Hydrates carbons (saccharides) found in nature (animals and plants) are classified into three groups based on the number of monosaccharides found or released upon acid hydrolysis. Monosaccharides the groups of carbohydrates.
3.1 Formulation of Monosaccharides
The molecular formula of any monosaccharide (mono=one and saccharide=sugar) is Cn(H2O)n or (CH2O)n. Rhamnose and Deoxyribose are exceptions to the general formula of carbohydrates. Acetic acid (C2H4O2) and formaldehyde (CH2O) are not carbohydrates but have the formula Cn(H2O)n or (CH2O)n. Fitting and Baeyer proposed the formula CHO-(CHOH)4-CH2OH, which indicates the presence of aldehyde and five hydroxyl groups.
More than one monosaccharide molecule unites to form disaccharides, oligosaccharides, and polysaccharides. Monosaccharides, being simpler units, cannot be broken down further. Generally, they have two to ten carbon atoms per molecule (dioses to decoses). The monosaccharides may have either aldehyde or ketone groups. The one possible aldodiose (CH2OH.CHO) is glycolaldehyde (2-hydroxyethanal), but ketodiose is not possible since there are only two carbons. The glucose molecular formula C6H12O6 represents the number of C, H, and O atoms but not groups. However, the proposed formula, CHO-(CHOH)4-CH2OH, indicates the presence of an aldehyde and five hydroxyl groups. These simple sugars or monosaccharides have carbon backbones linked by single bonds with hydrogen and hydroxyl groups attached in an unbranched manner.

Carbohydrates are the most abundant class of organic compounds found in living organisms. Carbohydrates are also called sugars and are found in plants and animals. Plants synthesize them through photosynthesis (endothermic) by fixing carbon, hydrogen, and oxygen in the presence of solar energy and chlorophyll pigments.
nCO2 + nH2O + energy → CnH2nOn + nO2
Carbohydrates are stored as starch in plants as a source of energy. They are also stored in animals as glycogen in muscles and the liver. They are also found in skeletal structures of cell walls in plants (cellulose), bacteria (peptidoglycans), insects, and crustaceans. This stored food is consumed by animals and man, and upon their oxidation in the body, energy is released for various metabolic activities (work, growth, and various productive functions). Compositionwise, plants are more prosperous in carbohydrates (30%) than animals (1%), with water at 60% and ash at 4%. Plants are poor in proteins (5%) and lipids (1%) as compared to proteins (20%) and lipids (15%) in animals.
2.1 Introduction to Carbohydrates
Carbohydrates or saccharides (sakcharon=sugar or sweetness) are a chemical term applied to a large group of substances, which are organic compounds containing elements Carbon, Hydrogen, and Oxygen in a ratio of 1:2:1. Hydrogen and Oxygen atoms are in the ratio of two to one as in water molecules. As the ratio of hydrogen to oxygen is like H2O, they are also known as “hydrates of carbon” and are represented as (CH2O)n. But there is an exception to this general formula. Deoxyribose (C5H1OO4) and rhamnose (C6H12O5) do not have the required ratio of 2:1 for hydrogen to oxygen. On the other side, some compounds possess this required ratio but are not carbohydrates, e.g., formaldehyde (CH2O), acetic acid (C2H4O2), and lactic acid (C3H6O3). Further, some carbohydrates have nitrogen(glucosamine), phosphorus, and sulfur but do not satisfy the above general formula. The simplest sugars are called monosugars or monosaccharides. There are two main functional groups in

1.1 Carbohydrates Biochemistry
Carbohydrates are essential for life, both in plants and in animals. They are organic chemical compounds made of C, H, and O. These elements get arranged in different ways so that several simple, and stored complex compounds (starch in plants and glycogen in animals) are available in abundance on the Earth. They are involved in enormous biological and nonbiological functions. Their biological functions, found in all living things, are the chief energy source for men and animals, even for herbivorous fishlike carp and rohu fingerlings. Carbohydrates are also essential structural components like nucleic acids, containing genetic information. One must recognize the protective function in cells, plants, and human health management. Even bacteria form a glycocalyx and biofilm for protection. Knowledge about their involvement in blood groups helped to identify universal donors, universal recipients, and genuine donors for the recipient to save a life. Carbohydrates are found as a storage structure for energy in emergency conditions.
Upon combustion, they yield carbon dioxide and water. Autotrophs (plants) synthesize them from carbon dioxide and water to grow, reproduce, and store, but heterotrophs (humans and other animals) use them, too. In plants, they occur as fibers, supporting structures, and simple sugars. The plant carbohydrates are used for preparing adhesives, fermentation, foods, and papers. Cotton fibers are used to make clothes to cover the body and protect humans and animals against adverse climatic conditions.

Experiment No.- 1
AIM
To determine the sucrose content in condensed or evaporated milk using the Lane-Eynon method.
Principle
The Lane-Eynon method is used to measure sucrose in condensed milk by utilizing the reducing properties of sugars. Sucrose, being a non-reducing sugar, is first hydrolyzed into glucose and fructose (reducing sugars). These reducing sugars then react with Fehling’s solution, producing a brick-red precipitate of cuprous oxide (Cu2O) when heated. This allows for the accurate determination of sucrose content after the hydrolysis (inversion) process.
Reactions
• Reaction of Fehling’s Solution
• CuSO4 + NaOH → Cu(OH)2 + Na2SO4
• Cu(OH)2 + Reducing Sugar (RCHO) → Cu2O (brick-red precipitate) + RCOOH
This reaction forms cuprous oxide (Cu2O), a brick-red precipitate. The intensity of this precipitate correlates with the concentration of reducing sugar in the sample, providing a way to determine sucrose content.

4.1 Introduction
Human milk widely acknowledged as the optimal source of nutrition for infants, providing a rich array of components crucial for their growth, repair and development. However, the composition of human milk can vary
significantly within and between feeds, throughout the day, over the course of lactation, and among different mothers and also populations. While this variability is beneficial for infant health and survival, it presents challenges for the management of nutrition of high-risk infants who require precise oversight. The composition of human milk can be altered by factors such as the handling of expressed milk, including how it is stored and pasteurized. Thus, proper management of expressed milk is essential. The recommended practice for infant nutrition is exclusive breastfeeding for the first six months, followed by continued breastfeeding for at least one to two years. Human milk is naturally suited to meet the nutritional needs of infants, providing both nourishment and bioactive compounds that support healthy growth and immunity.
Human milk contains a variety of bioactive components, such as immune cells, anti-infective and anti-inflammatory agents, growth factors, and prebiotics. In contrast to infant formula, which has a fixed nutritional profile, human milk is dynamic and can vary between and within feedings, influenced by maternal factors, environmental conditions, and the methods used for expressing and managing milk.
A thorough understanding of human milk’s composition is vital for effective infant feeding, especially for at-risk infants. It also allows for the assessment of how milk storage and pasteurization may impact its beneficial components. Additionally, some of the bioactive elements in human milk are being studied for their potential use in medical treatments, either as preventive or therapeutic agents.

3.1 Malted Milk
Malted milk, originating from the ideas of British pharmacist James Horlick in the 19th century, was initially marketed under the name “Diastoid” before being trademarked as “malted milk” in 1887. Although initially targeted
for infants and invalids, its appeal expanded due to its nutritional value, convenience, digestibility, and palatability. Recognized globally for its lightweight, non-perishable nature, and high-calorie content, malted milk is
created by evaporating a mixture of malted barley, wheat flour, and whole milk until it forms a powder.
There aremainly two types of malt powder: diastatic and non-diastatic. Diastatic malt containing enzymes breaking down starch into sugar, aiding in dough rise and crust formation, particularly in bread making. Non-diastatic
malt lacks active enzymes and is primarily utilized for flavor enhancement, commonly in beverages. It may also include sugar, coloring agents, and other additives to improve taste and appearance.
3.2 Malting
Malting is a crucial process employed in treating cereal grains, such as barley, by inducing germination through soaking in water followed by halting the process through drying or heating with hot air. This technique encompasses two main stages: sprouting and kiln-drying. The term “malt” encompasses several products of this process:
1. Malted grains: These are grains, like barley, that have undergone the malting process.
2. Malt sugars: These are sugars, predominantly maltose-rich, derived from malted grains, often utilized in baking, such as baker’s malt.
3. Malted milk products: These include beverages like malted milkshakes, made using malted milk.