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Technology transfer (TT) is entering a transformative phase shaped by globalization, digitization, and the growing role of start-ups in the innovation ecosystem. Traditional linear models of TT where inventions flowed from academic laboratories to industry through licensing are being replaced by dynamic, multi-directional, and collaborative frameworks. This chapter explores emerging trends that will define TT in the coming decades, including open innovation platforms, digital TT ecosystems powered by artificial intelligence (AI) and blockchain, the rise of entrepreneurial start-ups and spin-offs, internationalization of TT through cross-border collaborations, and sector-specific shifts driven by 5G, biotechnology, drones, and green energy. Drawing from global and Indian contexts, the chapter highlights how universities, governments, and industries can leverage these trends to build inclusive, resilient, and sustainable innovation ecosystems.
Three global shifts which will redefine TT
1. Digitization of knowledge: With AI, big data, and blockchain, knowledge and IP can be shared, tracked, and commercialized faster and more transparently.
2. Entrepreneurial ecosystems: Start-ups, incubators, and accelerators are replacing traditional firms as vehicles of commercialization, particularly in deep-tech and frontier technologies.
3. Globalization of innovation: Challenges such as pandemics, climate change, and food security require cross-border TT collaborations, making TT an instrument of both economic and geopolitical significance.

Introduction

The world does not function without mathematics. In our day-today life we come across many problems which are solved mathematically. The mathematical logical system follows “Yes” or “No”, “True” or “False”, “1” or “0” which are precise outputs for the precise inputs. There are several ambiguous or vague problems which cannot be given with accurate precise value with the conventional logical operators. Beyond this Boolean logic operator the logical system which mimics the human brain and gives precise solutions for the imprecise linguistic variables is the Fuzzy logic system. Fuzzy logic is a novel approach that intends to imitate human reasoning using calculations and operations with fuzzy systems and linguistic variables. The problems with vague imprecise data which we come across in our day today life cannot be solved by conventional binary logical method. The fuzzy logic solution spontaneously splits a complex problem into its component parts. This reduces the solution to its component parts. Compilers may re-assemble the code for effective execution on some target, but at the programmer level the problem is a number of simple tasks.

Fuzzy sets theory was introduced by Zadehin the year 1965, and it allows uncertain phenomena to be treated mathematically.

Galactometer: It is an instrument used for recording the specific gravity of the milk (Plate 306).

Galactose: It is an aldohexose sugar obtained by hydrolysis of lactose. Galactose is a monosaccharide and has the same chemical formula as glucose, i.e., C6 H12 O6 . It is similar to glucose in its structure, differing only in the position of one hydroxyl group. Due to this difference galactose possess different biochemical properties to glucose (Plate 307).

Galactosidase: An enzyme (actase) that hydrolyzes a galactoside. β-Galactosidase, commonly known as lactase, is an enzyme responsible to hydrolyze lactose (Plate 308).

Galactoside: A glycoside that yields galactose on hydrolysis. A galactoside is a glycoside containing galactose. The H of the OH group on carbon-1 of galactose is replaced by an organic moiety (Plate 309).

Galactosyl: A glycosyl radical C6 H11 O5 that is derived from galactose.

Fisheries as a source of food, livelihood and income is probably as old as human civilization. Millions of men and women across the world, especially the developing world, are dependent on the sector. While fish capture in the open seas has generally been a male preserve conditioned by various social, cultural and economic factors; women also have significant contributions for sustaining household nutritional and income security. They have been engaged in fishing in inshore coastal waters, inland water bodies like rivers and ponds; and in post harvest activities like pre-processing, processing, drying, salting, and allied activities like marketing, net making and mending etc. With increasing volumes of fish coming from culture fisheries the participation of men and women in these activities are also increasing. Suffice to say that both men and women contribute to the overall growth and development of the fisheries sector. However, often women and their contributions tend to be marginalized in the fisheries development debate.

Women’s roles in fisheries

In the marine fisheries sector, fishing is largely a male preserve. Women rarely venture into the sae, conditioned by cultural and social taboos rather than by skill and endurance. However, the support from women in managing the households and in taking up subsistence or other livelihood activities related to fisheries activities that may actually go into the household income security need to be acknowledged. Studies show that women comprise about 46% of the labour force in small-scale capture fisheries-related activities (FAO, World Bank, IFAD, 2008). It is as high as 73% in Nigeria to a low of 4% in Mozambique. At least 50 % of the workforce in inland fisheries and 60 % of those marketing fish are women in Asia and West Africa.

The term ‘gender’ was first used by Ann Oakley and others in 1970s to describe the characteristics of men and women which are socially determined in contrast to biological differences. The term ‘gender’ further explains, a neutral term meaning, either men or women or both are in a particular context. The goal is to improve the status of disadvantaged class and get rid of socially created and approved discriminations. The green revolution in India changed the face of agriculture but apparently it contributed to two general trends (FAO, 1996) where wealthy have benefited more from technological change in agriculture than the less well off and secondly men have benefited more than women. In general, it is true that the priorities are set by men and some women, who are unaware of gender issues, and that these are seldom considered unless the research is sociological. But even social research can be totally gender blind.

1. Proteins in solution absorb ultraviolet light with absorbance maxima at 280 and 200 nm. Amino acids with aromatic rings are the primary reason for the absorbance peak at 280 nm. Peptide bonds are primarily responsible for the peak at 200 nm.
2. Aromatic amino acids tyrosine and tryptophan give proteins their characteristic ultraviolet (UV) absorption at 280 nm, which is routinely used to estimate protein concentration. Phenylalanine and disulfide bonds also contribute to absorption at 280 nm, albeit slightly.

1.1. Introduction

The significance of milk as the most wholesome single food available in nature has never been debated. It is as ancient as mankind itself, as it is the substance created to feed the mammalian infant. All species of mammals, from the lowly rat to the mighty whale, produce milk for this purpose. Several centuries ago, perhaps as early as 6000-8000 BC, ancient man learned to domesticate species of animals for obtaining their milk for consumption. These included cows, buffalos, sheep, goats and camels, all of which are still used in various parts of the world for the production of milk for human use (Fig. 1.1 & 1.2).

Nature has made such a beautiful system that a fruit does not spoil unless its outer covering is removed and till it is there on the plant. For example banana will not spoil for long time if we do not remove its peel. Similar is the case with apple, guava, mango and many other fruits and vegetables. But as soon as the outer covering is removed the damage begins and the commodity is spoilt very soon.

Preservation is extending shelf life of fresh or processed food by using various methods and prevention or reduction of spoilage. Better the preservation, lesser the spoilage, more the spoilage poorer and inefficient is the preservation.

Therefore, in order to prevent or reduce the spoilage, we have to first identify and understand the causes of spoilage. There are chiefly 3 agencies that cause spoilage in fruits and vegetables i.e. (i) microorganisms, (ii) enzymes & chemical changes and (iii) man, animals, rodents, insects, etc. For effective preservation we have to check the growth and activity of these spoilage causing agencies. So, in order to prevent spoilage and preserve a perishable commodity for long time the following principles and methods are to be followed.

Abstract
Understanding genetic diversity is essential for achieving genetic improvement and conservation of grain legumes. These crops serve as important pulses grown and consumed all over the world, especially in Africa, South East Asia and America. Legumes serve as a good source of carbohydrates, oil, fibre and proteins. Grain legumes are a major source of food and nutrition globally. However, it has not been possible to enhance gain legume production to the required level, primarily due to the narrow genetic base of most of the legume crops, coupled with changing climatic conditions. Most grain legumes are lacking in desired plant ideotypes and resistance sources for various biotic and abiotic stresses. The genetic diversity conserved in gene banks globally is a major resource for crop breeding programs, but it
is utilized only marginally. Therefore, in order to broaden the genetic base of grain legume crops and enhance the genetic gains made in improvement programs, conventional approaches and modern scientific tools should be integrated in a phased and carefully judged manner. The first phase should focus on the search for desired traits and the infusion of diversity into the cultivated gene pool through use of landraces and CWRs. The second phase should focus on the utilization of advanced selection tools, such as genomics, high-throughput precision phenotyping, and artificial intelligence, to exploit the hidden potential of the available genetic diversity; and the third phase should involve technologies such as mutational breeding, genome editing, and transgenic technologies to improve, modify any novel or alien traits that are not available in the entire crop gene pool. We presume that enrichment of the genetic diversity of cultivated grain legume gene pools, along with simultaneous improvements in their yield and plant type with the aid of advanced scientific tools, will enhance grain legume crop yields to the required level.
Keywords: Crop improvement, Genetic diversity, Genome editing, Legume, Phenotyping

Abstract

The genetic improvement of crop plants is possible only through employing an appropriate breeding technique. The use of plant breeding technique depends on mode of pollination and reproduction of plant species e.g. selection and hybridization are used in sexually reproduced species, while clonal selection in asexually propagated plant species. The plant species/ varieties are usually derived through conventional techniques (vi z. introduction, selection, hybridization etc.), which are commonly used in various breeding programmes, while some modern techniques viz. transgenic breeding and marker assisted selection etc. are also available which are equally effective for improvement of all crop species. Various techniques applied for development of new varieties and hybrids are briefly described in this section.

Plant breeding is an art, science and technology of improving genetic make-up of crop plants in relation to their economic usefulness for mankind. Various techniques/methods of breeding used for genetic improvement of crop plants are: introduction, selection, hybridization, mutation, transgenic breeding etc.

Classification of Breeding Techniques: Plant breeding techniques are classified as conventional and modern breeding techniques.

Even after public concern the scientists feel that the GMO foods are quite safe. According to them the genetically-engineered crops are very safe to eat as much as the non-GE of the same crop. According to them the environment has improved by reducing the use of pesticides. GMO stands for genetically modified organism. Soy, corn, or other crops are very common as GM crops which are made by genetically engineered DNA. Many have come to the conclusion that GM foods are safe, healthy and sustainable. However, some people are against them. There are many benefits of GM crops like less water or pesticides to be used. Genetically engineered crops fight diseases to save the foods. At the same time the industries/ farmers that grow them are also benefitted. These foods have higher nutritional profiles, both in the US and abroad like corn which is one of the most prominent GMO foods. Soy is found in tofu, vegetarian products, soybean oil, soy flour and many other products. The other major crops taken for genetic modification are Papayas, Canola and Cotton. For dairy products a lot of work is being done on the genetic engineering of animals in a big way. This is going to be the most intractable environmental challenge of the 21^st Century. Presently, around 92% of the U. S. corn (GE), 94% of soybeans and 94% of cotton are genetically engineered.

GENETIC ENGINEERING

Genetic engineering is a means of improving the food supply even before harvest or slaughter by improving yields, increasing disease resistance, and enhancing the nutritional qualities of various foods. Broadly speaking, genetic engineering refers to any deliberate alteration of an organism’s DNA. Genetic engineering has been practiced for thousands of years, ever since humans began selectively breeding plants and animals to create more nutritious, better tasting foods. In the past two decades, genetic engineering has become increasingly powerful as scientific advances have enabled the direct alteration of genetic material. Genetic engineering is alternately called as ‘ recombinant DNA technology’ or ‘gene cloning’. Genes have been cut and pasted from one species to another, yielding, for example, disease- resistant squash and rice, frost-resistant potatoes and strawberries, and tomatoes that ripen-and therefore spoil-more slowly.

Genetically modified organism (GMO) is a microbe or more complex plant or animal in which DNA has been altered for a particular purpose such as manufacturing proteins, making improvement to the organism, research into the nature of genes and biological process, correcting genetic defects.

Millets are the staple food cropsacross the globe. The ability to thrive in harsh conditions established them as most suitable for current climate adversaries. The nutritional richness of millet reminded the need for adding them to our food basket.Despite possessing several desirable traits their cultivation is limited to a few pockets across the globe.Though significant progress has been achieved by conventional and transgenic breeding programs inmillets, there is a need for rapid improvement to meet the current needs.In order to accelerate the pace of breeding efforts, several molecular biology and biotechnology tools are being deployed by plant breeders. In the meantime, genome editing techniques like CRISPR, TALENS, ZFNs, Base and Prime editing have revolutionized the methods of genome engineering among which CRISPR has emerged as the most rapid, convenient and effective mechanism. The availability ofwhole genome sequence information, identified candidate genesfor various traits and successful transformation methods of millets promoted the application of CRISPR and resulted in the improvement of several traits. Initially, its application has been limited due to the restricted policies across the globe but the gradual change in the policies promoted genome editing across several parts of the globe. Thus, the application of genome editing for millet crop improvement can bring back those ancient grains to our food basket.

Diversity of gut microbial community

The human microbiota (the collection of microbes that live on and inside us) consists of about 100 trillion microbial cells that outnumber our ‘‘human’’ cells 10 to 1, and that provide a wide range of metabolic functions that we lack. It has been estimated that they encode 100-fold more unique genes than our own genome. Furthermore, the gut microbes contribute to energy harvest from food, and changes of gut microbiome may be associated with bowel diseases or obesity. To understand and exploit the impact of the gut microbes on human health and well-being it is necessary to decipher the content, diversity and functioning of the microbial gut community. If we consider ourselves as superior organisms encompassing these microbial symbionts, by far the majority of genes in the system are microbial. In this sense, completing the human genome also requires the characterization of the microbiome (the collection of genes in the microbiota).

Methods of counting viable and non-viable bacteria in gut

Before the advent of genome sequencing and genome-wide analysis method, our knowledge of molecular characteristics of diverse gut microbial population was extremely limited. To obtain an estimate of cultivability, microbial ecologists generally compare microscopic counts with total viable counts. The total viable count made on a nonselective agar based medium estimates the number of colony forming units (cfu) per gram of sample. The finding that total viable counts are typically lower than total microscopic counts was thought to be due to the number of dead cells. Indeed, dead bacteria in feces may constitute up to one third of the total bacterial community. However, recent nucleic acid based studies indicated that a majority of bacteria in a variety of ecosystems are different from those described in culture.

Introduction
Ghee is the pure clarified butter fat prepared chiefly from cream and/or desi butter (Makhan) to which no colouring matter is added.  Ghee manufacture has great significance and relevance to Indian masses and the dairy industry. Ghee is a very popular product since ancient time and has greater demand during festival and other ceremonial functions where use of ghee in food has been considered to delicacy due to its pleasing flavour and aroma. There are sufficient recorded evidence to prove that the manufacture of ghee originated from India and has been used extensively for religious and dietary purposes since Vedic times. Presently about 28% of total milk production is utilized for manufacture of about 1 million tones of ghee per annum. It is the traditional product with an established market. Cow ghee is golden yellow in colour, and is attributed unique therapeutic value.  The colour of buffalo ghee is greenish yellow due to presence of pigments called billirubin and billiverdin. Ghee is characterized by its pleasant, cooked and rich flavour. The preferred texture is of larger uniform size from grains having non-greasy consistency.

Ginger is used extensively as a spice in cuisines throughout the world. Ginger belongs to Zingiberaceae family and an English botanist William Roscoe (1753-1831) gave this plant the name Zingiber officinale in the year 1807. The name of the genus Zingiber derives from a Sanskrit word denoting “horn shaped” in reference to the protusions on the rhizomes (Ghosh et al., 2011). It is an oldest rhizome widely domesticated as a spice where the edible part is the swollen underground stem or rhizome. The Zingiberaceous plants have strong aromatic and medicinal properties and are characterized by their tuberous or non-tuberous rhizomes (Chen et al., 2008). Ginger is a medicinal plant that has been widely used all over the world, since antiquity, for a wide array of unrelated ailments including arthritis, cramps, rheumatism, sprains, sore throats, muscular aches, pains, constipation, vomiting, hypertension, indigestion, dementia, fever and infectious diseases (Ali et al., 2008). Ginger has direct anti-microbial activity and thus can be used in treatment of bacterial infections (Tan and Vanitha, 2004).

5.1. Introduction

Glass is one of the oldest packaging materials used for food. The use of glass as a packaging material dates back to 3000 BC where it was the first object to be used for containing and carrying food objects. It is made by cooling a heated fused mixture of silicates, lime and soda. It is an amorphous material in which all the constituents individually form an irregular network at microscopic level. It means it has failed to form a definite crystalline structure during cooling. Glass is defined as “amorphous, inorganic product of fusion that has been cooled to a rigid condition without crystallizing (ASTM, 2010)”. It has no definite melting point.

Global onion research connects crop domestication, genetic diversity, nutrition, therapeutics, production, breeding and post-harvest technology. Onion contains organosulphur compounds, quercetin derivatives and other phytochemicals associated with antimicrobial, antioxidant, cardioprotective and anticancer effects. Despite high global production, productivity differs substantially among countries, and storage losses remain a major economic concern. This chapter reviews global production trends, germplasm resources, breeding strategies, phytochemistry, therapeutic potential and emerging storage technologies. Landraces, cultivars and wild relatives provide valuable traits for yield, storability and stress resilience. Advanced treatments such as ozonation, cold plasma and irradiation demonstrate promise, although wider commercial adoption, economic validation and regulatory development remain necessary.

1. Key Forces Shaping Food Packaging • Changing Consumer Priorities • Modern consumers are increasingly choosing food packaging that aligns with values of health, convenience, and sustainability. The demand is growing for packaging options that are recyclable, biodegradable, or reusable. Consumers also expect transparency, with clean labels and QR codes providing details on ingredients and sourcing, which has become essential for building trust (Santeramo et al.,2022) • Environmental Sustainability • Sustainability remains a powerful influence on packaging innovation. Reducing single-use plastics, increasing recyclable options, and adopting circular economy principles are all priorities. Both consumers and businesses are actively seeking solutions that reduce environmental impact, such as renewable, compostable materials and designs that minimize waste ( Fao et al ., 2022)