eChapters

During processing seeds are cleaned, dried, and graded which are then treated with specific fungicide and insecticide. Seed at this stage is at best quality, which are then packaged into various packaging materials in a specific weight. Packaging or bagging of processed and treated seed is a requisite for easy handling of the bulk seed. Seed packaging can be defined as filling with seed, weighing and sealing the container whereas seed package as storage container where the seeds are kept for marketing or shipping or till the next sowing time.

The Packaging of Seed Involves

1. Filling bags/ containers of uniform size with seeds of known weight.

2. Providing leaflets inside the package with information about any specific cultural practices needed.

3. Attaching labels or certification tags having information regarding all the quality parameters.

4. Temper-proof sealing of the bag.

5. Storage or transportation of the bags.

Food Contact Materials (FCM)

Before food consumption, during production, processing, preparation, packaging, packing, transportation, storage, serving etc. food comes in contact o many materials, all these materials are called food contact materials (FCM). FCM may be containers used for transportation for example milk container, machineries for its production or processing, packaging materials for its packaging like cans of soft drinks, kitchenware used for its cooking, serving, heating, washing. The selection of FCM requires intense care because FCM can affect human health and also the quality of food. During contact with food, molecules can migrate from FCM to food. Hence, FCM should have the following common attributes:

1. FCM should be compatible with the materials used for production, processing, transport, storage, serving etc.

2. FCM should be inert and non-reactive.

3. FCM should withstand the chemical, Mechanical and Thermal variations.

4. FCM should be clean, hygienic and tamper proof.

5. FCM should not have blisters, cracks, sharp edges and visible defects. 6. FCM should be free from rust.

Sensory evaluation is a scientific method of evaluating foods including meat products that applies principles of experimental design and statistical analysis through the use of human senses like sight, smell, taste, touch and hearing. It is also called organoleptic evaluation in which we measure, analyse and interpret reactions to those characteristics of materials as they are perceived by the human senses. These attributes can be perceived well by the human sense organs as compared to instruments available for this purpose. It is a fact that no single instrument is able to assess all the attributes of meat products. With the help of these instruments we can very well evaluate the single quality attribute of meat product i.e. shear force instrument or penetrometer is good for knowing the texture and refractometer is for colour interpretation.

Concept: A few of the numerous environmental elements that affect seed quality maintenance are moisture, temperature, humidity, and storage conditions. When some seedborne diseases or pests like insects are taken into consideration, seed quality can still be negatively impacted. Applying one or more pesticides to seed is the most economical and efficient way to keep pests away and improve seed quality.

Because pesticides are harmful, handling treated seeds after application requires additional caution and safety measures.

Definition of Treated Seed

The definition of “treated” is “application of a pesticide or subject seed to a process intended to reduce, control, or repel insects, disease organisms, or other pests which attack the seed or seedlings.”

Food Safety and Standard Authority of India (FSSAI) has adopted the standards of Fruit Product Order (FPO).

Chutney

It is a product prepared by cooking the fruit pulp with added salt, sugar, spices, acetic acid and fruit to a suitable consistency so that it contains TSS not less than 50% and fruit part not less than 40% as per FSSAI specification. Sugar, salt, spices, acetic acid all act as partial preservatives. Preservatives may also be added in chutney for increasing its storability. Generally no artificial colour and flavor are required to be added.

Sauce

Sauce is a product similar to ketchup, prepared from pulps of tomato or other fruit/vegetable, having TSS not less than 15% and cooked to a suitable consistency with added sugar, salt, spices and vinegar. Sugar, salt, spices, acetic acid all act as partial preservatives. According to FSSAI specification fruit sauce should have minimum of 15% TSS and 1.2% acidity. Preservatives and colours may also be added for increasing of appearance and storability.

In India after rice wheat (Triticum aestivum L.) is the second most important cultivated food crop belonging to family poaceae. Total production of wheat was 760 million tons in India during 2016-2017 (FAOSTAT, 2017) which is second largest in the world after china. In India highest producing state of wheat is Uttar Pradesh followed by Punjab, Haryana, Madhya Pradesh, Rajasthan etc. Wheat is categorize into seven commonly known wheat classes those are hard red spring, hard red winter, soft red winter, hard white, soft white, durum and mixed wheat (Khamis, 2014). 

Introduction

A variety of glassware, plasticware, and other apparatus are used for experimental purposes in a laboratory. They need to be washed, cleaned, dried, and sometimes sterilized before they can be used in the next set of experiments. Glassware needs special care of handling during washing, drying, sterilization, etc. because even a minor mistake in handling of glassware may result in its cracking/breakage and it may become unserviceable. All of these may result in a delay in experimentation if spare piece of the glassware is not available or it may affect serviceability of the associated apparatus.

A large number of glassware such as beaker, flask, measuring cylinder, burette, test tube, etc. are regularly used in a laboratory, which requires careful handling during the different stages of experiment as well as after completion of the experiment while preparing them for another set of experimentation.

Glass being a complex silicate, its properties depends on the type of silicate anions in the structure and the cations formed. Thermal properties of glass changes by the addition of boron oxide (B2 O3 ); hence, borosilicate glass is extensively used in the laboratories. With this brief background information, let us discuss about the necessity, procedures, and precautions during washing, cleaning, drying and sterilization of laboratory-wares. Figure 3.1 shows various advantages of using borosilicate glass in a laboratory.

Good laboratory practices require clean glassware, because an experiment may give erroneous result if dirty glassware is used at any step of the experiment. Hence, the glassware must be physically and chemically clean; and in many cases, it must be microbiologically clean or sterile. The commonly used glassware in a laboratory include test tube, measuring cylinder, pipettes, beakers, flasks, microscopic slides, etc.

Introduction

Entrepreneurship is crucial for economic development, especially during crises when job losses push people to find alternative livelihoods, often turning to entrepreneurship as a means of survival. Its impact is highly beneficial, promoting growth, wealth creation, and better living standards. In developing countries like India, entrepreneurship offers a path to holistic development, particularly for disadvantaged groups traditionally dependent on agriculture. Uplaonkar and Biradar (2015) noted that entrepreneurship development effectively generates employment, increases income, reduces poverty, improves health, and ensures food and nutritional security. Recognizing the importance of entrepreneurship, stakeholders in the agricultural sector now see its positive impact on business sustainability. Verhees et al. (2011) argued that promoting entrepreneurship in agriculture can significantly reduce poverty and boost rural prosperity, directly addressing socio-economic challenges in rural areas (Bhooshan and Ranjith, 2022). Therefore, supporting the entrepreneurial ecosystem in rural sectors through programs and schemes is essential.

Overview of the Indian Dairy Sector

India’s dairy sector occupies a unique global and domestic position due to its scale and socio-economic impact. As the world’s largest producer of milk with 230.6 million tonnes of production in 2022-23 (BAHS, 2023), India contributes approximately 23% of global milk production, surpassing countries like the United States and New Zealand (FAO, 2020). This dominance is largely attributed to Operation Flood, the revolutionary initiative launched by National Dairy Development Board (NDDB) in the 1970s, which transformed India from a milk-deficient country to the world’s largest milk producer (World Bank, 1998). Operation flood is known for its contribution in increasing milk production, supplement rural income and make available reasonable prices to the consumers. This initiative laid the foundation for the cooperative model of dairy production, epitomized by the success of organizations like Amul, which became a global model for dairy development (Candler & Kumar, 1998).

Abstract

The objectives of the study is to help to create self-employment and provide proper information for different conventional milk based products. Traditional dairy foods have always played a crucial role in preservation of precious milk nutrients and promotion of its use among masses. The demand for milk and milk products is increasing with increasing of world's population. To meet these needs, high-end technology is needed for emerging the capability of milk production and preserving it by producing high quality milk products. The dairy industry is highly localized owing to perishable nature of milk products. Approximately 50% of the total milk produced in India has been projected to be processed into a variety of conventional milk products, such as khoa, chhena, paneer, dahi, ghee, kalakand and a wide range of sweets in rural areas mostly by the rural citizens. Therefore, setting up a milk-based food production system and updating conventional technologies to manage milk-based products on a commercial scale is very important.

Introduction

Measurement of pH of a solution and preparation of different types/strength of buffers are common practices in a laboratory, particularly in the Biochemistry and Molecular Biology laboratories. Therefore, in this chapter you will be acquainted with the procedures of measuring pH of a solution as well as preparation of some of the commonly used buffers in a laboratory.

Potential or Power of Hydrogen Ion (pH)

Measurement of pH of a solution is measuring the molar concentration of hydrogen ion [H+] in the solution, which is an indication of the acidity or basicity of the solution. The symbol pH stands for “power of hydrogen” and numerical value for pH is just the negative logarithm of the molar concentration of hydrogen ions [H+]. The ionic product of water (Kw ) is the basis for the pH scale. The total hydrogen ion concentration from all the sources and it can be experimentally measured.

The pH scale designates the concentration of H+ (and thus of OH−) in an aqueous solution, which ranges between 1.0 M H+ and 1.0 M OH−. The term pH is defined by the expression

Thus, the ionic product of water makes it possible to calculate the concentration of H+, with given concentration of OH−, and vice versa. The value of 7 for pH of a neutral solution is not an arbitrarily chosen figure; it is derived from the absolute value of the ionic product of water. A solution having pH greater than 7 is basic, and the concentration of OH− is greater than that of H+. A solution having a pH less than 7 is acidic.

Onion growth, bulb initiation, yield, quality and storage life are strongly influenced by temperature, photoperiod, soil, moisture and crop establishment. Different cultivars require specific day lengths and temperature ranges, making regional variety selection and planting schedules essential. This chapter explains the environmental requirements of onion and the consequences of unsuitable conditions, including premature bolting, double bulbs, poor development and disease susceptibility. It examines soil preparation, planting geometry, direct seeding, transplanting and precision establishment. Modern approaches—including sensor-based environmental monitoring, Internet of Things systems, predictive modelling, precision seeding and machine-learning-supported input management—are presented as tools for improving uniformity, resource efficiency and climate adaptability.

Introduction

Different types of culture media are used for culturing microbes and plant tissues. Preparing as well as sterilizing them properly following appropriate method with safety for the desired purpose are important parts of research work. In this chapter, we will discuss briefly about different types of media, their composition, preparation and sterilization in laboratory for microbial or plant tissue culture.

Culture Medium

Ingredients mixed to prepare solid, liquid or semi-solid substance to support the growth of microorganism or plant tissue in vitro (outside the body) is called culture medium. Frequently, it becomes important to grow microorganism or plant/animal tissue in vitro for any or all of the following purposes:

• To study characteristic features or properties of microorganisms.

• To identify the causal organism(s) of infection in a clinical sample, so that appropriate remedy can be determined.

• To culture/multiply microorganism or plant/animal tissues in vitro for research or commercial purposes.

• To prepare/produce biological molecules, vaccines, antigens, proteins, metabolites, etc. in vitro for research or industrial purposes.

SYNOPSIS

Fish has now become an important animal protein for mankind. It is a rich source of essential amino acids, vitamins and minerals. Fish meat is easily digestible compared to other animal meat and has a higher Protein Efficiency Ratio when compared to casein, the milk protein. Fish oils like body oils of fishes like sardine, mackeral, anchoviella, herring, salmon etc. are the richest source for Omega-3 long chain poly unsaturate fatty acid like Eicos- pentanoic acid (EPA) and Docosahexaenoic acid (DHA) having therapeutical applications for man. A number of fishery products and by-products like squalene, chitin, chitosan, oyster meat, mussel meat, shark bones etc.

Glycolysis involves a series of reactions to extract energy from glucose to
release two molecules with three carbons called pyruvate. The process is not
dependent on oxygen but can occur under aerobic and anaerobic conditions,
releasing different end products. Under aerobic conditions, the pyruvate formed
is then oxidized in the mitochondria to CO2 and H2O. The energy released in
glycolysis is in the form of ATP (High Energy Bond). All the reaction steps
occur in the cytoplasm, as it is the extramitochondrial pathway. In anaerobic
conditions and the absence of mitochondria (RBC), the end product of
glycolysis is lactic acid.
The net reaction of Glycolysis (anaerobic) may be summarized as under
Glucose + 2ADP + 2Pi → 2 Lactate + 2ATP
7.1 Steps in EMP Cycle or Glycolytic Pathway
Table 7.1: Steps and enzymes of glycolysis or EMP cycle.

Introduction

Flowering plants (Angiosperms) are identified by their morphological characteristics like flower, fruit and seeds that help their taxonomic characterization into different species, genera, families and orders. The floral characters which are used for taxonomic characterization of plants include the type of inflorescences, perianth structure, floral symmetry, arrangement of floral leaves, type of androecium, stamens, gynoecium, carpel, ovule, etc. The type of fruit and seed also play important roles in taxonomic classification. In this chapter we will briefly discuss about various morphological features of flowering plants (in botanical terms) that are useful in taxonomy.

Nomenclature of Plant

Nomenclature of plant is the formal and scientific naming of plants. It is related to but distinct from taxonomy for grouping and classifying plants and provide botanical name.

The scientific/botanical nomenclature started with Species Plantarum by Carolus Linnaeus in 1753. Botanical nomenclature is governed by the International Code of Nomenclature for algae, fungi, and plants (ICN), which replaces the International Code of Botanical Nomenclature (ICBN).

ICBN and ICN

International Code of Botanical Nomenclature, (ICBN) was adopted by the VIIth International Botanical Congress held in Stockholm, July1950. While the International Code of Nomenclature for algae, fungi, and plants (ICN) was adopted by the XVIIIth International Botanical Congress held in Melbourne, Australia in July 2011.

Introduction

We come across a variety of chemicals every day in a laboratory. While some of them are relatively safer, others are extremely hazardous. Thus, it is very important to know the potential hazards that can be caused by the chemicals present in the laboratory and the safety measures needed to be followed to avoid the hazards. Additionally, we should also be aware of the steps necessary to be taken on such hazardous situations. According to IUPAC, a hazardous chemical mean the chemical which has the potential to cause damage in various ways. In a laboratory, there are chemicals that might be irritant, carcinogen, respiratory fibrogen, systemic poison, asphyxiant, flammable, etc. which have the potential to cause hazard to the workers or environment. Therefore, it is important to use appropriate procedure to reduce the risk and protect the health/safety of laboratory personnel, the public and the environment.

Types of Hazards Associated with Chemicals

Chemical hazards: The hazards caused by chemicals can be broadly classified into three categories.

1. Health hazard: This is also known as toxic effects of chemical. The risk associated with a chemical in laboratory depends on the extent of exposure and the inherent toxicity of the chemical. Many chemical reactions form the product that might be much more toxic than the reacting chemicals. For example, inadvertent mixing of formaldehyde (a common tissue fixative) and hydrogen chloride results in the generation of bis(chloromethyl) ether, a potent human carcinogen. For most of the chemicals, the route of exposure (through skin, eyes, gastrointestinal tract, or respiratory tract) is more important (Figure 17.1) that must be considered during risk assessment.

Abstract

Modern agriculture largely depends on use of high cost inputs and energy intensive farm machinery. Worlds increasing population and continuously deteriorating soil fertility has alarmed agriculture system to look for some safe alternatives. Organic manures are rich source ofnutrients and humans, therefore crop residue recycling by composting is a best alternate of modern high cost input based agriculture. Compost is an aerobically decomposed organic matter .Bacteria, fungi, actinomycetes algae and protozoa are involved in the process of composting and are found throughout the natural environment. The composting material warms up due to microbial activity and it’s temperature becomes up to 160 ⁰F active stage of microbial activity . There are four types of compositing i.e. Windrow composting, Aerated static pile composting, In-Vessel composting and Vermi-composting. The C: N ratio, aeration, temperature and moisture are major factors affecting composting process. Major advantages of composting are: Aggregate formation, Reduction in bulk density, Increase in hydraulic conductivity, Reduction in loss of soil moisture, Increase in organic carbon content and pH of soil.

Introduction

Proper electrical wiring and earthing are crucial for laboratory safety and functionality. A laboratory houses several installations/equipment that demand stable/continuous power supply. Inappropriate or faulty wiring and earthing may lead to significant risks, including electrical shocks, damage to equipment, and even incidence of fire. Therefore, understanding the requirements of electrical wiring and earthing is essential for designing, construction, and maintenance of laboratory. This chapter aims to provide a basic knowledge of electrical wiring and earthing necessary for a laboratory. Importance of earthing in mitigating electrical hazards and ensuring integrity of electrical installations are also described.

Basics of Electrical Wiring

Basic understanding of electrical wiring is necessary for laboratory safety and efficient electrical functioning. The potential difference between two electrical points (negative and positive) is known as Voltage, which drives the flow of electric current. This is measured as volt (V). The rate of flow of electricity in a circuit is known as Current (I), which is measured as ampere (A). Current can be either direct (DC) or alternating (AC). Opposition/resistance experienced in the flow of current in circuit is known as Resistance (R) and it is measured in ohms (Ω). Higher the resistance means lesser the current flows at a given voltage. The rate at which electrical energy is transferred/utilized in an electric circuit is known as Power (P), which is measured in watt (W). The Power can be calculated as the product of voltage and current (P = V × I).

Structure

Structural unit of edible portion of most fruits and vegetables is the parenchyma cells and makes up bulk of cells. A parenchyma cell is rather thin walled and may be polygonal or cubical in shape.  Walls of parenchyma cells in young plants are composed almost entirely of fibrils of cellulose. In parenchyma cells of plants actively metabolising protoplast portion represents only about 5% of total cell volume. This protoplast is rather film like and is pressed against cell wall by large water filled central vacuole.  Protoplast has inner and outer semi permeable membrane layers between which is confined cytoplasm and its nucleus.