eChapters

Introduction

Several hazardous chemicals, microbes, and techniques like recombinant DNA technology are used for research and development work in research Institutes and universities. Therefore, the researchers, technical, helping and supporting staffs as well as the laboratory outputs must be kept free from contaminations. Thus, biosafety is necessary to prevent unintentional exposure/ release of hazardous chemicals, microbes/pathogens, and toxins to the laboratory staff and environment. Following the biosafety guidelines and ethical considerations are essential to maintain safety standards for the researchers as well as the environment. This chapter deals with various biosafety measures and ethical issues to be strictly followed while working in a research laboratory. The required safety level needs to be determined as per the chemical/biological materials are used in the laboratory to keep the researcher/environment safe for sustainable research and developmental activities.

Biosafety

The term biosafety is used to describe the procedure and policies needed to ensure environmental and personal safety. Biosafety refers to the containment principles, technologies and practices that are implemented to prevent unintentional exposure to pathogens, toxins, or their accidental release into the environment. A fundamental objective of any biosafety program is the containment of potentially harmful chemical/biological agents as precautionary safety measures.

Why Biosafety?

With the increasing number of countries adopting molecular biology tools and techniques in life science research and development activities, especially in the areas of agriculture and medicine, the biosafety issues are gaining importance to ensure biological safety for the public and the environment. Conducting research in safe and sustainable manner is not only a personal requirement but essential collective efforts to ensure biological safety for a clean and safe environment. This requires rules, regulations, monitoring bodies, and awareness among the workers as well as the public. Therefore, biosafety per se is an integral part of laboratory research and requires awareness among the researchers so that biological safety can be well taken care from the grassroot level.

13.1    Biochemical Changes During Banana Ripening
    
13.2    Commercial Ripening

Introduction

Introduction to Feasibility Reports

A feasibility report is a detailed analysis of the viability, sustainability, and profitability of a proposed project. It is crucial for decision-making and project planning, particularly in the dairy industry where multiple variables affect the outcomes.

Objectives of Feasibility Reports • Assess economic viability.

• Analyse technical requirements.

• Evaluate operational sustainability.

• Provide a roadmap for project implementation.

• Decision-Making Tool: Helps stakeholders decide whether to proceed with a project. Hence, minimize risks through identification of potential obstacles and informed decision-making.

• Resource Optimization: Ensures efficient use of resources like time, money, and labour.

• Stakeholder Communication: Provides a structured overview of the project’s potential.

Carbohydrates (glucose) are the primary energy source for a body; when digested, glucose is the end product in human beings and simple stomach animals, but it yields volatile fatty acids in ruminants. Precise regulation of levels of blood sugars/glucose in the body is the net result of various processes like digestion, absorption, and metabolism operating under the interaction of various a) enzymes and b) hormones secreted by the different organs of the body. An abnormal secretion of any of the enzymes or hormones associated with the metabolism of carbohydrates disrupts the precise regulation of blood sugar, leading to various disorders.
14.1 Enzyme Deficiency and Disorders in Carbohydrate Metabolism
Usually, various enzymes are involved in the breakdown of carbohydrates, and if their secretion is insufficient, missing, or does not work correctly, it leads to different disorders.
I. Glycogen storage diseases (GSDs, glycogenosis, and dextrinosis)
Glucose is needed by the cells for the generation of energy and other cellular activities. If glucose happens to be in excess, it is stored as glycogen, mainly in the liver and muscles, for future needs. The stored glycogen can be converted back to glucose when the need arises. Many enzymes are related to this interconversion of glucose and glycogen. Deficiencies of one or more enzymes may lead to abnormal glycogen metabolism, leading to glycogen accumulation of normal or abnormal chemical structure in tissue, collectively called glycogen storage diseases (GSDs). Roman numerals identify different types of glycogen storage conditions. They are also classified under various categories like inherited metabolic diseases, autosomal recessive type, and X-linked type (VIII/IX type).

Over the past several years, there has been significant change in consumer perception on quality and food safety. Consumers are demanding healthy, nutritious and cost-effective products processed under controlled hygienic conditions. Food safety concern, in recent years, has become dominant and important. The requirement of food safety and quality assurance has never been so demanding as it is now. Globally food safety is being addressed independent of food quality. In anticipation of consumer demands on food safety, food sanitary standards at national and international levels are under constant review. Several inter governmental agencies / organizations are engaged in work related to quality assurance and food safety. Amongst these World Health Organization (WHO) and Codex Alimentarius Commission (CAC) play the major role in protecting public health and defining standards of quality and safety aspects of foods for regional and or world wide usage.

Regulatory Institutions

The quality environment in the Indian food processing sector revolves around compulsory legislation which specify minimum standard and certification system. Legal standards are generally made to have control over the quality of foods offered for sale and to safeguard the consumer from health hazards posed by practice of adulteration. There are basically three types of Indian standards namely

India has a population of one hundred twenty four crore plus and feeding this large a population calls for a strong FPI. The industry also assumes importance in the national economy due to the immense employment generation opportunities it offers and the export revenue it earns. Food Processing Industry (FPI) in India is a sunrise sector that has gained prominence in the recent years. Easy availability of raw materials, changing lifestyles and favourable fiscal policies has given a considerable push to the industry’s growth. FPI serves as a vital link between the agriculture and manufacturing sectors of the economy. Strengthening this link is critical to reduce wastage of agricultural raw materials, improve the value of agricultural produce by increasing shelf-life as well as by fortifying the nutritive value of the food products and ensure remunerative prices to farmers as well as affordable prices to consumers. The industry has very low processing level i.e., 2.2% for fruits and vegetables, around 35% in milk, 21% in meat and 6% in poultry products, which is significantly lower by international standards. For e.g., processing of agriculture produce is around 40% in China, 30% in Thailand, 70% in Brazil, 78% in the Philippines and 80% in Malaysia. Value addition to agriculture produce in India is just 20% with wastage estimated to be valued at around US $13 billion.

India has the second largest producer of food with arable land of 159.65 million hectares and has the highest acreage under irrigation. Next to China, India ranks second largest food producer in the world and has the potential to immerge the biggest with its food and agricultural sector. India accounts for less than 1.5% of international food trade despite being one of the world’s major food producers, which indicates huge potential for both investors and exporters. Food and food products are the biggest consumption category in India

SYNOPSIS

Urban and rural agriculture are different entities in terms of stakeholder involvement and enabling environment for farming but they are dialectically interlinked in providing food and nutrition security of any country. Urban agriculture is more suitable on the production of nutritious crops, while rural agriculture’s first priority is to produce more food from staple. Attaining nutrition security is more complex than it is previously thought. However, while existing systems of production should continue at field levels by developing more nutritionally rich cropping patterns, its integration with farmers’ farming systems remains more important.

Synopsis

Food safety has become increasingly important with globalization due to various factors such as increased volume and diversity of trade in foods, greater public demand for health protection, changing hazards e.g., resistant microbes, more sophisticated methods for detection of hazards, changes in production and consumption pattern, etc. Food safety contributes significantly to food security as unsafe and poor quality food leads to illnesses as well as food wastage. This leads to a strong need to emphasize not only on availability of food but also ensure that the same is safe.

Introduction The method of drying is expressed as applying heat under controlled conditions to remove the water or moisture from foods to get a dry product with low moisture content. The primary commitment of drying is to extend the shelf-life of foods by diminishing their water activity. Due to low water activity, microorganisms which cause food spoilage and decay and many of the enzymes which promote undesired changes in the chemical composition of the food are unable to grow, multiply or function. The need for drying in the food industry is increasing day by day, and Novel dehydration techniques are being invented for a more efficient drying process, less energy-consuming, and preserve the product characteristics and nutritional profile. Further, the drying technology is critical for moisture removal from food and non-food materials. Small scale farmers use this food preservation method as an indigenous method and the food processing industry as modern technology. Dried products have numerous benefits compared to raw products, including an extended shelf-life and reduced packaging, storage, handling, and transportation costs. However, drying is a highly energy-intensive food processing operation.

4.0. Introduction

Quality is a measure of the degree of excellence or degree of acceptability by the consumer. By quality, one can differentiate the individual unit of a product from the other units and can determined the degree of acceptability of the individual unit by the consumer. The quality may also be defined in term of end use and may vary depending upon consumer’s perception and need. According to USDA Marketing Workshop Report (1951) “quality is the combination of attributes or characteristics of a product that have significance in determining the degree of acceptability of the product to the user”. For industry, quality is a measure of purity, strength, flavour, colour, size, maturity, workmanship and conditions or any other distinctive attributes of the product.

According to Code of Federal Regulations “Quality is the inherent properties of any processed product which determine the relative degree of excellence of such product and includes the effect of preparation and processing and may or may not include the effects of packaging or added ingredients/ additives”.

In India, people are mostly vegetarian, depending largely on cereals and pulses, as their staple foods, which provide the main source of dietary proteins and calories. The proteins from pulses are known to be inferior quality, due to the deficiency of sulphur containing amino acids as well as due to other factors like digestibility, availability of amino acids, anti-nutritional factors, etc. The anti-nutritional factors present in this type of food, including their contents of enzyme inhibitors, lectins, flatulence factors, tannins, phytic acid and saponins. Anti-nutritional factors can cause detrimental effects to humans and animal growth and performance by impairing intake, uptake, or utilization of other food and feed components, or by causing discomfort and stress to humans and animals. The anti-nutritional factors mainly occur in pulses and grain legumes and foods and feed material prepared from grain legumes and pulses.

Substance which, from nutritional point of view, interferes with normal growth, reproduction or health when consumed regularly in amount existing in a normal component of diet should be considered as harmful and toxic. A significant part of human population relies on legumes as staple food for subsistence, particularly in combination with cereals. They are unique foods because their rich nutrient content including starch, protein, dietary fibre, oligosaccharides, phytochemicals (especially the isoflavones in soybean) and minerals. Their nutritional contents contribute to many health benefits to human.

Advancements on sugar processing

India has popped out as one of the largest sugar processing country throughout the world. Through the collaboration of research and development carried out in the field of sugar processing, this industry has witnessed the vast changes in term of technology. Important considerations such as productivity improvement and cost reduction were the main objectives in the field of sugar processing. Sulphur dioxide was earlier used but afterwards lime treatment in clarified juice helped in the processing of white sugar from sugar cane. Since then the continuous progress and improvement has been made and advanced techniques are introduced for manufacturing of sugar with modern tools.

Ramjeawon (2004) reported that farmers and agriculture workers grow sugar cane and beet root for sugar production,providing about 20 kg per capita and that is equivalent to almost 13 percent energy requirement. Fechter et al. (2001) reported that production of the white sugar was focused more on juices rather than molasses which were taken as the raw material. Various processes such as ultra-filtration, de-mineralization and de-colorization were used for the production of white sugar. The traditional refining process wascheaper, and effective in terms of purification and juice purification was the method used for the processing of white sugar directly from the sugar cane. Crystallization was one of the process which is used for increasing the recovery of sucrose and it reduces the molasses production. Chromatography was used in the sugar industry for the separation of sucrose. Another method was ion exchange demineralization which was used in the sugar industry for the removal of minerals from the liquid sugar. Later adsorption has been incorporated to be used de-odorization and de-pigmentation. Membrane filtration is latest technique used in the process which was used for maintaining the quality of white sugar by removing unwanted particle from the juice. Velásquez et al.,(2018) reported that thermal processes are also used for the production of sugar from sugar cane. Non centrifugal cane sugar processing is used for this purpose and the product is obtained by evaporating the sugarcane which has the unique flavor and aroma. Later the product is used as the sweetener and has a traditional characteristics flavor and optimum nutrition.

Introduction

Probiotics, prebiotics and synbiotics are interrelated terms. Probiotic is discussed in detail in previous chapter. In this chapter, prebiotics and its effect on gut along with probiotics are emphasized. Prebiotics can be defined as non digestible food ingredients that stimulate the growth and/or activity of bacteria in the digestive system in ways claimed to be beneficial to health. They were f irst identified and named by Marcel Roberfroid in 1995. He defined prebiotic as a selectively fermented ingredient that allows specific changes, both in the composition and/or activity in the gastrointestinal microflora that confers benefits upon host well-being and health. Further, FAO defined prebiotic as “a non-viable food component that confers a health benefit on the host associated with modulation of the microbiota.”Whereas the term synbiotics refers to a product that contains both probiotics and prebiotics in the form of synergism. A synbiotic has been defined as “a mixture of prebiotics and probiotics that beneficially affect the host by improving the survival and implantation of live microbial dietary supplements in the gastrointestinal tract, by selectively stimulating the growth and activating the metabolism of one or a limited number of health promoting bacteria, and thus improving host welfare”. As described above, the combined use of prebiotics and probiotics is often described as synbiotic, but the United Nations Food & Agriculture Organization (FAO) recommends that the term “synbiotic” be used only if the net health benefit is synergistic.

This chapter introduces artificial intelligence, machine learning, computer vision and spectroscopy for rapid, automated and non-destructive egg-quality assessment. It covers machine-vision fundamentals, image acquisition and preprocessing, feature extraction, machine-learning models, deep learning, convolutional neural networks, hyperspectral methods and defect detection. Applications include crack detection, freshness prediction and internal-quality assessment. The chapter also examines model validation, transfer learning, sensor integration, commercial grading-line requirements and the practical limitations of deploying AI systems at industrial scale.

Abstract

Classification of the hundreds of possible dryers is essential to the logical selection of appropriate dryer for a given application. Diverse criteria for classification as well as selection of dryers are presented and discussed briefly. The advantages and limitations of different dryer types must be taken into consideration. Initial capital cost is often the key deciding criterion in industrial dryer selection. This is not a good strategy as the user must pay for the operating costs over a long period of life of the dryer. In general several different dryer types are suitable for a given product. Careful selection is essential for cost-effective operation of the dryer.

1.    The basic building block(s) of proteins is/are

(a) Amino acids

(b) Phenolics

(c) Carboxylic acids

(d) Fatty acids

2.    Alpha helix and ?- pleated sheet are characteristic of protein structure?

(a) Primary

(b) Secondary

(c) Tertiary

(d) Quaternary

Introduction In today’s environment, the greatest challenge for the food industry is to develop health promoting foods; it is determined by the raw materials used and its impact on consumer health. Furthermore, people lifestyle has changed greatly, resulting in a change in food eating patterns. In today’s world, people are moving towards eating foods that are nutrient base and tasty (Bonciu, 2018). of all the nutrient based products available in market, expanded snacks have become a popular snack all over the world because of its crunchy texture and low density making it consumer’s acceptance (Grasso, 2020). The basic idea for developing any expanded snacks is utilizing a starch-based material for a better crispy and crunchy product, but to make it more nutritious incorporation of protein and fibre rich raw materials is recommended (Gu et al., 2017). Along with the raw materials, the other important parameters for developing any extruded product is the particle of the flour, its physicochemical and rheological properties of the extrudates are much concentrated (Bouasla & Wójtowicz, 2021) Thereby, extruded snack is considering as functional food with major health benefits.

1.    A lipid is

(a) Insoluble in water but soluble in nonpolar solvents

(b) Insoluble in both water and nonpolar solvents

(b) Soluble in water but insoluble in nonpolar solvents

(d) None of the above

2.    Fats are

(a) Esters of fatty acid with glycerol

(b) Esters of fatty acid with long chain monohydroxy alcohols

(c) Both (a) and (b)

(d) Esters of fatty acid with alcohols

Introduction

Let food be thy medicine and medicine be thy food- the age-old quote by Hippocrates, is certainly the tenet of today. Microbial cultures have been used for millenniums in food and alcoholic fermentations and in the past century have undergone scientific scrutiny for their ability to prevent and cure a variety of diseases. Later it became popular in improving human and farm animal nutritive values and recently in aquaculture also. The growing interest in the understanding the role of food in human health has moved from its primary role as a source of energy to the subtle action of biologically active food components on human health. Henceforth functional foods or nutraceuticals are of great demand in present time. The growing scientific evidence suggests that the food supplements containing beneficial bacteria can provide an array of benefits to the host. Now a day’s probiotic fermented foods are in great demand due to the awareness of beneficial aspects of probiotics among people. Several industries focused on the improvement of conventional fermented foods scientifically in order to fetch good market for their products. Particularly, animal feed companies and researchers have been looking for alternative products and strategies that can help to maintain animal gut health in order to prevent or reduce the prevalence of pathogens in the food chain. After understanding the mechanism and beneficial aspects of probiotics in detail at molecular level in earlier chapters, now it is relevant to discuss the applied part of probiotics. In this chapter, commercial importance of probiotics and probiotics derived products at industrial level are emphasized.

Abstract

Hot air circulation is the most common industrial method of drying foods which has its own inherited disadvantages. Microwave drying can be an alternative method for removing moisture from foods and there has been a surge of interest in the application of microwave to process the foods, in past few years. The reason being that there is worldwide energy crisis and the growing acceptance and familiarity with domestic microwave ovens. It is well known that conventional means of heat processing irreversibly alter the flavor, colour, and texture of many foods. From the point of flavor alone, it is desirable to improve our present methods of processing foods. Some improvements have been made in this direction with a number of food products by use of high-temperature short- time processing.