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Experiment No.- 1
AIM
To determine the sucrose content in condensed or evaporated milk using the Lane-Eynon method.
Principle
The Lane-Eynon method is used to measure sucrose in condensed milk by utilizing the reducing properties of sugars. Sucrose, being a non-reducing sugar, is first hydrolyzed into glucose and fructose (reducing sugars). These reducing sugars then react with Fehling’s solution, producing a brick-red precipitate of cuprous oxide (Cu2O) when heated. This allows for the accurate determination of sucrose content after the hydrolysis (inversion) process.
Reactions
• Reaction of Fehling’s Solution
• CuSO4 + NaOH → Cu(OH)2 + Na2SO4
• Cu(OH)2 + Reducing Sugar (RCHO) → Cu2O (brick-red precipitate) + RCOOH
This reaction forms cuprous oxide (Cu2O), a brick-red precipitate. The intensity of this precipitate correlates with the concentration of reducing sugar in the sample, providing a way to determine sucrose content.

Introduction

Food is a resource of vital importance and its safety in the whole chain, from producer to consumer, is a worldwide concern. In fact, as food trade expands throughout the world, food safety has become a shared concern among all consumers. This issue began to be taken seriously at a period where there is a significant increase of illness caused by infected food in both developed and developing countries. Unsafe food contains hazardous agents or contaminants that can make people sick, immediately or by increasing their risk of chronic disease. Contaminants can enter food at many different points in the food production process, and can occur naturally or as the result of poor or inadequate production practices. International competitiveness depends more on quality and safety concerns than on prices. Governments and local authorities intervene in the market place in setting technical regulations; and sanitary and phytosanitary measures. This encouraged food processing companies around the world to implement food safety management systems which guarantees the production and distribution of safe foods. Prerequisite Programs (PRPs) are considered as the integral part of food safety standards and audit schemes all over the world. It is essential to implement food safety programmes in every company to check the chances of illness or injury due to unsafe food.

1. INTRODUCTION

Since vedic times, importance of fermented milk and its products was known but the major attention to probiotic and its health related benefits was gained after Eli Metchnikoff’s publication entitled “The prolongation of life” in 1908. Due to the awareness of the role of nutrition in health and well-being, improved knowledge of diet-related diseases, consumer demand for probiotic and prebiotic products has been increased in recent years. The link between diet and health is flourishing the market for health-embracing population and opens an opportunity for Food and Dairy Industries meet the demand of this market. Probiotics, constituting live micro organisms, have been incorporated into drinks, food products, supplements (tablets, capsules and freeze–dried preparations) and prebiotic products. Milk, milk based products, fruits and vegetables are rich in functional food components such as minerals, vitamins, dietary fibers, and antioxidants (phytochemicals). Application of probiotic bacteria in such products further enhances the nutritional value of the food product. Probiotic juices produced from fruits and vegetables have also been in consideration in recent years. The food market is being getting captured by pro- and prebiotic food products day-by-day. Even the agro-industrial by-products such as whey, wheat straw, rice straw, sugarcane bagasse, etc. are being explored for the economical and cost effective production of prebiotic molecules. Thus, this chapter gives a comprehensive overview of pro- and prebiotics and their market potential.

Abstract

The concern of consumers for convenience and healthy foods involves many parameters like functional and nutraceutical properties, ease to prepare, good quality, economics as well as good nutritional profile of the food. This trend of convenience has been increasing in today's world because of the societal changes like the aging of population, the increasing entry of women into the labor force, the change to a multi-cultured society and the increasing polarization between poor and rich. Value addition to the traditional sources is a medium to bring variety products into the market. Cereal starches possess a wide application in the food industry as an additive but the native starch is not widely used in food industry due to its poor functional properties. Pregelatinized starches and flours have become important in processed foods because the functional properties of the starches and flours are improved over the native starches and cereal flours. Modification is a process which alters the structure of the starch in the wheat endosperm by affecting the hydrogen bonds in a controllable manner. Modification of starch and flours is generally done by several methods such as chemical degradation, physical alteration or enzymatic transformation. The primary objective of modification is to reduce the hot viscosity of the starch paste so that higher concentrations of starch can be dispersed without excessive thickening. The pregelatinized flours offer a tremendous number of functional benefits to variety of foods such as bakeries, snacks, beverages as well as nutritional foods. The various functional benefits of pre-gelatinized starch/flour as an additive are classified as fat replacer, texture improver, stabilizer, emulsifier, thickening agent and for freeze-thaw stability. Keywords: Pregelatinization, modification, functionality, cereal flour.

Introduction

Quality of fish is affected by various factors, which are classified into two categories: intrinsic factors and extrinsic factors. Intrinsic factors are the chemical and physical properties inherent to fish. Extrinsic factors are treatments given to fish before and after catch until it reaches the consumer, which includes the method of catch, cross contamination, processing, preservation, and product storage environment (temperature, humidity, packaging). The processing methods such as drying, smoking, chilling, and salting greatly help to minimize the spoilage. The quality of fish is also dependent on cold chain, in which the temperature is managed and monitored at every stage of production, transport, storage and sale.

2.1. Pre-Harvest Factors

Pre-harvest factors relates to the intrinsic factors affecting the quality of fish, as they are beyond the direct control of human. Various intrinsic factors affecting quality are fish species, size, sex, physiological state, parasites, naturally toxic fish, pollutants, and occasional peculiarities. Additionally, some of the extrinsic factors like method of catch and feeding interruption for starvation are also considered as pre-harvest factors affecting the quality of fish.

2.1.1. Species Characteristics

The quality of fish is related to species. For instance, flat fish keep better than round fish. Post-mortem pH varies between species. For example, long rigor period and low pH (5.4-5.6) of very large flatfish and halibut give relatively long iced storage life of 21 days, while mackerel with low post-mortem pH has no effect on shelf life. Some fish are rated as good quality as they are expensive, example, seer fish and pomfret. Fatty fish are rejected sensorial before lean fish due to the appearance of oxidative rancidity. So, lean fish keep longer than fatty fish under aerobic storage. Bony fish are edible longer than cartilaginous fish. Skin of fatty pelagic fish is very thin and contribute to faster

spoilage rate, as it allows enzymes and bacteria to penetrate more rapidly. Thick skin and slime of flatfish contribute for better keepability, as slime contains bacteriolytic enzymes, antibodies and other antibacterial substances.

Fruits represent a vital component of global agriculture, serving as not only a significant source of essential nutrients but also a lucrative sector of the economy. However, from the moment of their inception on the tree to their arrival on consumers’ tables, fruits undergo a complex journey influenced by a myriad of factors. Among these, pre-harvest practices wield a profound impact on fruit quality, enzymatic activities, texture, ripening, and ultimately, post-harvest transportation losses. Each pre-harvest practice, from mineral nutrition to harvesting methods, plays a distinct role in shaping the biochemical composition and physical attributes of fruits, ultimately affecting their marketability and shelf life. Mineral nutrition stands as one of the foundational pillars of fruit production, with essential minerals serving as building blocks for various biochemical processes. Irrigation practices represent another critical aspect of pre-harvest management, as water availability profoundly impacts fruit development and composition. Different irrigation regimes can influence enzymatic activities, fruit texture, and the timing of ripening, necessitating strategic water management to achieve desired fruit quality while minimizing post-harvest losses. Canopy regulation, achieved through training and pruning techniques, offers growers a means to manipulate light penetration and airflow within the orchard. Moreover, the application of plant growth regulators (PGRs) emerges as a tool for fine-tuning fruit development and ripening processes. By modulating hormonal signals, PGRs can alter enzymatic activities, influence fruit texture, and mitigate transportation losses. However, judicious application is paramount to avoid adverse effects on fruit quality and safety. Harvesting decisions, including the determination of optimal maturity and the timing and method of harvest, profoundly impact fruit quality and post-harvest losses.

Pre-processing of fish

Fish is a highly perishable food which requires proper handling and preservation to increase its self life and retain its quality and nutritional aspects. Therefore, fish pre-processing and preservation are become a very important part of commercial fisheries. Preservation means keeping the fish after landing in a condition of wholesome and fit for human consumption for a particular period. This preservation should cover the entire period from the time of capture of fish to its sale at retailer’s counter. The main aim of processing and preservation of fish is to prevent fish from deterioration. Methods which are commonly used to preserve fish and fishery products include the control of temperature using ice, refrigeration or freezing and control of water activity by drying, salting, smoking or freeze-drying etc (FAO, 2011). Fish has to be pre-processed in such a manner that it remains fresh for a long time with a minimum loss of flavour, colour, taste, odour, nutritive value and digestibility. Fish processing can be subdivided into fish handling which is the preliminary processing of raw fish and the manufacture of fish products.

Introduction

Quality is very important in any production and operation system. It ensures whether the products produced or the services provided are of the right quality in right quantity at right time. Quality management must ensure proper quality of the product to survive in the market and expand its market. Quality of the product must be given importance to avoid quality failures that cause serious human inconveniences and wastage of money.

5.1. Definitions and Terminologies

5.1.1. Quality

We say a product is of satisfactory quality, if it satisfies the consumers. According to C. D. Lewis, a British writer, quality is “an asset which is offered to the potential customer of that product or service”. The International Organization for Standardization (ISO) has put up a more comprehensive definition of quality as “the degree to which a set of inherent characteristics fulfils requirements” (ISO 9000: 2005 – 3.1.1). In general terms, ‘quality’ is defined as the composite of characteristics that differentiate individual units of a product to determine its acceptability by the customer. Quality characteristics are nothing but elements that makes a product fit for use. Quality characteristics are further classified into categories called as parameters.

5.1.2. Total Quality Management

Total Quality Management (TQM) is an organization’s management approach, centered on quality. It improves the effectiveness and flexibility of a company or an organization. TQM is defined as “the system of establishing defect prevention actions and attitudes with a company or an organization on a permanent basis for the purpose of assuring conforming products or services directed at customer satisfaction”. TQM is based on the participation of all its members in decision making, which is aimed for long-term success, through customer satisfaction and benefits to the members of the company or organization and to the society.

Pre-Treatment Before Sowing

The natural conditions necessary for seeds to germinate are increased by pre sowing seed treatment. Many plants and trees’ seeds require a specific amount of time to mature in their immature portions and to overcome dormancy, which is the absence of certain elements that impede germination, so that the seeds can sprout. Depending on the type of seed, different environmental factors promote different levels of germination. For example, some seeds need to be kept in a very damp, cold environment, while others need to be kept in a dry, hot environment. Different techniques are employed to support seed survival and speed up germination, depending on the size of the lot, the species, the local conditions, and the availability of pertinent tools and facilities. Many species’ seeds don’t germinate successfully unless they are exposed to specific circumstances. Dormancy is the state in which seeds do not germinate unless certain circumstances are met. Conditions in the natural world could include being near fire or being eaten by animals.

Concept: A few of the numerous environmental elements that affect seed quality maintenance are moisture, temperature, humidity, and storage conditions. When some seedborne diseases or pests like insects are taken into consideration, seed quality can still be negatively impacted. Applying one or more pesticides to seed is the most economical and efficient way to keep pests away and improve seed quality.

Because pesticides are harmful, handling treated seeds after application requires additional caution and safety measures.

Definition of Treated Seed

The definition of “treated” is “application of a pesticide or subject seed to a process intended to reduce, control, or repel insects, disease organisms, or other pests which attack the seed or seedlings.”

Abstract

Prebiotics are the non-digestible food ingredients that beneficially affect the host by selectively stimulating the growth and/or activity of one or limited number of bacteria in the colon. These are heat resistant components which remain intact during the baking process; this allows prebiotics to be incorporated into every day food choices. Consumption of non-digestible ingredients allows the growth ofbio cultures by reaching the intestine unaffected by the digestion process resulting in improvement of digestive health. Prebiotics reach the intestine in an unaltered form and shows the prebiotic effect when there is an increase in the activity of healthy bacteria in the human intestine. The prebiotics stimulate the growth of healthy bacteria such as bifidobacteria and lactobacilli in the gut and increase resistance to invading pathogens. Functional foods increase consumer choice by adding prebiotics to every day food items such as cereals, biscuits, breads, table spreads, drinks, and yoghurts. By continuing to eat and drink common foods, but choosing functional alternatives (such as bread containing prebiotics) dietary requirements can be met, without significant changes to foodpreferences. Some common example of cereal based products containing prebiotics are oat-based prebiotic drink, Breakstone's and Knudsen live active cottage cheese, prebiotic yogurt- Attune and Muller vitality, multi-fruit drink enrich and flavoured water enrich, prebiotic flavoured water, prebiotic capsules, dried fruit bars, prebiotic powder- Biocare fructo-oligosaccharides, Newtree purplaisir chocolate spread. The claims are constipation relief, suppression of diarrhea and reduction of the risks of osteoporosis, atherosclerotic cardiovascular disease associated with dyslipidemia and insulin resistance, obesity and possibly Type-2 diabetes. At present, claims about reduction of disease risk are only tentative and further research is needed.

Introduction

Probiotics, prebiotics and synbiotics are interrelated terms. Probiotic is discussed in detail in previous chapter. In this chapter, prebiotics and its effect on gut along with probiotics are emphasized. Prebiotics can be defined as non digestible food ingredients that stimulate the growth and/or activity of bacteria in the digestive system in ways claimed to be beneficial to health. They were f irst identified and named by Marcel Roberfroid in 1995. He defined prebiotic as a selectively fermented ingredient that allows specific changes, both in the composition and/or activity in the gastrointestinal microflora that confers benefits upon host well-being and health. Further, FAO defined prebiotic as “a non-viable food component that confers a health benefit on the host associated with modulation of the microbiota.”Whereas the term synbiotics refers to a product that contains both probiotics and prebiotics in the form of synergism. A synbiotic has been defined as “a mixture of prebiotics and probiotics that beneficially affect the host by improving the survival and implantation of live microbial dietary supplements in the gastrointestinal tract, by selectively stimulating the growth and activating the metabolism of one or a limited number of health promoting bacteria, and thus improving host welfare”. As described above, the combined use of prebiotics and probiotics is often described as synbiotic, but the United Nations Food & Agriculture Organization (FAO) recommends that the term “synbiotic” be used only if the net health benefit is synergistic.

The current world population of 7.6 billion is expected to reach 8.6 billion in 2030, 9.8 billion in 2050 and 11.2 billion in 2100, according to United Nations (UN). With roughly 83 million people being added to the world’s population every year, the upward trend in population size is expected to continue, even assuming that fertility levels will continue to decline (UN, 2017). This means there will be an extra billion people to feed within the next decade. Continuing population and consumption growth will mean that the global demand for food will increase for at least another 50 years .The future’s food systems need to be resource efficient and sustainable. It is quite clear that the only way to grow more food is to increase the yield while saving the environment from further degradation. It is not just food, it is the four F’s – Food, Feed, Fiber and Fuel. Digitization has increased in importance for the agricultural sector and is described through concepts like Smart Farming (SF), Precision Farming (PF) and Precision Agriculture (PA).The only way to get higher yield from limited resources is by changing agriculture into what we call Precision Agriculture, Climate Smart Agriculture, or as I prefer to call it Water-Smart Agriculture”

Precision farming manages spatial and temporal variability within fields through targeted, data-driven interventions. Onion is particularly suited to precision management because its productivity and quality are sensitive to moisture, nutrients, environmental stress, pests and diseases. This chapter describes applications of GIS, GPS, variable-rate technology, IoT, cloud platforms, drones, remote sensing, multispectral imaging, smart sensors, artificial intelligence, automated systems and crop-response models. These technologies support soil assessment, field preparation, irrigation, fertilization, crop monitoring, stress detection, protection, yield estimation, harvesting and storage. Their potential to reduce input waste and improve profitability is considered alongside constraints involving investment costs, technical knowledge, connectivity, accessibility and data privacy.

According to Fereshteh et al. (2020) microorganisms use biofilm to protect themselves against the harmful effects of the host body immune system and the surrounding environment. This helps them to survive against the anti-microbial agents. Inhibition of bacterial biofilm formation is very time consuming and expensive. Newer computational tools have been found to be a good option to predict the inhibition of biofilm peptides. In a computational prediction tool a large number of peptide sequences can be screened to select the peptides for validation. Vectors are extracted from the peptide primary structure and classification algorithms are carried out which includes SVM, random forest and k-nearest neighbour to evaluate their performance. Using a combination of features, helps in good evaluation. National Institute of Health (NIH) has found out that 80% of bacterial pathogens form biofilms and their multicellular structure makes them resistant to the hosts’ defense systems and traditional antimicrobials than planktonic bacteria. Recently, the use of Antimicrobial peptides

Cordial is a sparkling, clear, sweetened fruit juice beverage from which pulp/ fibrous material has been removed completely. It contains at least 25% fruit juice, 30% total soluble solids (TSS), 1.5% acid and 350 ppm sulphur dioxide. Lemon and lime are the most suitable fruits for making cordial.
Recipe: The recipe for the preparation of cordial as a general guideline is as under:
Extract the juice from lime or lemon as in squash and store in glass bottles after adding potassium metabisulphite @ 1.0 g/kg of the juice. Allow the juice to settle for a month. Decant the clear juice without disturbing sediments and strain it through a fine muslin cloth. Prepare syrup by mixing sugar with water, boil, strain and allow to cool. On cooling, mix juice in syrup and strain the cordial through muslin cloth if necessary. Add KMS and fill into bottles. Stor in a cool and dry place.

Fruit nectar is a type of fruit beverage that contains not less than 20% fruit juice or pulp, not less than 15o B total soluble solids and acidity ranging between
0.3-0.5 % but not exceeding 1.5%/. For orange and pineapple nectar, the fruit juice shall not be less than 40%. The product is preserved by heat processing/ thermal processing, as such no chemical preservative is permitted in nectars. Unlike squashes, fruit nectar is served chilled as such without any dilution.
Mango, guava, pineapple, papaya, jamun etc., are the most suitable fruits for making nectar.
Recipe: As a general guideline, the following recipe is used for the preparation of fruit nectar:
Procedure
i. Select fully mature and ripe fruit and extract pulp as discussed under fruit pulp making but do not add any chemical preservative.

Fruit pulps are semi-finished products that are used for manufacturing finished value-added products like jam, ready-to-serve beverages, squash, appetizers etc. According to Food Laws, pulp refers to an unfermented but fermentable product, pulpy, turbid or clear, obtained by a mechanical process from sound ripe fruits/vegetables. These are preserved either by heat processing or by using chemical preservatives. The maximum preservative permitted under Indian Food Laws is 1000 ppm sulphur dioxide added in the form of potassium metabisulphite.
Mango, guava, apple, pineapple, papaya, bael, guava, peach, plum, apricot etc., are the most suitable fruits for making pulp for use in finished product preparation.
Procedure
i. Select fully mature and ripe fruit like mango, guava, apple, peach, apricot, kiwi, etc., and wash thoroughly.

Jam is prepared by boiling the fruit pulp with a sufficient quantity of sugar to a thick consistency, firm enough to hold fruit tissues in position. For the preparation of jam, not less than 45 parts of fruits are used for every 55 parts of sugar. The jam prepared by using two or more fruit pulps is called mixed fruit jam. Apple jam, apricot jam, mango jam, guava jam, pineapple jam, and mixed fruit jam prepared by using the pulp of two or more fruits are quite common. According to Food Safety & Standards Regulations,2011 the minimum TSS in the Jam shall not be less than 65%(w/w). Earlier the minimum limit was 68% (w/w)
Raw material, ingredients and utensils required
1. Fruits like mangoes, guava, apples, apricots, plum, peaches and kiwi etc singly or in combination
2. Stainless steel knives, de-corers, ladle, utensils for cooking and mixing, glass bottles (Jars), LPG or Diesel Bhatti, bucket/tub for washing, Stainless steel sieve, Steam Jacketted Kettle, Boiler (for the commercial unit) etc. 

Ready -to -Serve (RTS) drink is a type of fruit beverage that contains at least 10% fruit juice/pulp and 10o Brix total soluble solids with acidity ranging between 0.3-0.5%. The product is not diluted before serving like squash; hence it is called a ready-to-serve beverage. The product can be preserved by using 70 ppm SO2  or 120 ppm benzoic acid. Mango, orange, guava, lemon, lime, litchi, kiwi fruit, apricot, pineapple, etc., fruits are used for making RTS beverages. For lime & lemon drinks minimum level of juice content is 5% as per Indian Food Laws.
Recipe: As a general guideline, the following recipe is used for the preparation of ready-to-serve drinks:
Procedure
i. Select fully mature and ripe fruit like mango, guava, apricot, kiwi, etc., and wash thoroughly.
ii. Some fruits like mango, kiwi, pineapple, and litchi are peeled before heating. Apricots, guava, plums etc are not peeled. Place fruit after peeling in a pan/pressure cooker and boil till softening