eChapters

1.1 Introduction Millets were one of the first crops to be domesticated by mankind in Asia and Africa which later spread across the globe as critical food sources to the evolving civilizations. The earliest evidence of millets has been found in the Indus civilization around 3000 BC. Millets are a collective group of small-seeded annual grasses that are grown as grain crops, primarily on marginal land in dry areas of temperate, sub-tropical and tropical regions (FAO). However, due to the popularity of the cereal crops like wheat, rice and maize; millets have lost dominion and have become an underutilized crop in last few decades. Millets have the potential to be the crop of the future, as our major crops have reached yield plateau, also changing climate has introduced several new biotic and abiotic stresses to the major crops, so to feed the ever-growing population we need to shift towards other potential crops. India is the largest producer of millet as it contributes about 41% of the total millet production of the world (APEDA, 2020).

The shift from vegetative to reproductive phase in plants is the result of multiple gene cascades in response to photoperiodic and temperature dependent pathways, gibberellic acid pathway, vernalization pathway and autonomous pathway. The environmental cues are initially detected in the leaves and the mRNA of the flowering signal integrator FT gene, moving to the shoot apical meristem, acts as a long-distance signal for floral meristem identity. At SAM, FT-FD protein complex, based on signals from above said pathways in association with the signals from age-dependent and miRNA pathways, leads to the activation of master regulator gene of flowering AP1. The activation of genes involved in ABCDE model of floral organ differentiation, based on the directions of various cadastral genes lead to the development of floral organs. Thus, the sequential action of floral meristem identity genes, floral organ developmental genes, floral meristem size and organ number genes, floral organ pattern genes and cadastral genes leads to the flower development. This chapter sums up the gene interactions happening in leaf and SAM, leading to the development of a complete flower.

Endophytic Microorganisms

Endophytes (‘endo’, inside; ‘phyte’, plant) include microorganisms that thrive inside the plant tissues without any obvious adverse effects on the host, and they include mainly bacteria and fungi (Hallmann et al., 1997; Bacon et al., 2002; Hardoim, 2015). Endophytic microorganisms share an intimate association with the host adding essential and functional capabilities. Therefore, plant may be considered as a superorganism composed of an amalgam of both selected symbiont microbiota and host cells (Hardoim, 2015) with the new concept of holobiome, i.e., plant together with the associated microorganisms collectively governing the plant activities (Hardoim et al., 2015; Vandenkoornhuys et al., 2015).

Food Safety has been the buzz word in recent days as there are increasing consumer awareness on hazards present in food as well as the ombudsmen role played by independent media. Although regulatory regime across the world has taken proactive steps, in most of the cases it has been a knee-jerk reaction to the impending crisis. Defining the actual goal of food safety has been an arduous task as there are umpteen interrelated factors that influence the intended goals. Some of the definitions on food safety put forward by international agencies are as follows:

Storage : It can be defined as keeping the commodity fresh or processed in safe condition with minimum of deteriorative changes for later use.

Reasons of Deterioration During Storage

Preservation of food (fruits and vegetables) in common salt sand vinegar is called pickling. It is one of the most ancient method of preservation of fruits and vegetables. Pickling is the result of fermentation by Lactic acid forming bacteria which are found in large number on the surface of fruits and vegetables. They can grow in acidic medium and in the presence of 8-10 percent salt solution. The growth of majority of undesirable bacteria is inhibited. Lactic acid most active at 30 °C temperature. This temperature should be maintained during the process of pickling.

Preparation of pickles

Pickles are prepared with salt, vinegar and oil or combination of these ingredients. Spices are also used for pickle preparation.

INTRODUCTION

India is endowed with a vast and rich diversity of natural resources particularly soil, water, climate and agro-biodiversity. To realize the optimum potential of the agricultural production system on a sustainable basis, there is a need to bestow a paramount importance in the arena of efficient management of natural resources. India with a geographical area of 329 M ha presently supports 17% of the world’s population on merely 2. 5% of world’s land area and 4% of world’s freshwater resources. India’s economy is predominantly agrarian in nature with about 72. 2% of its 1028 million population (census 2001) living in villages and about 54. 4% people (cultivators) directly depending on agriculture.

INTRODUCTION

India has varied agroclimatic conditions, which favour the cultivation of various horticultural crops. Based on the adaptability and system of cultivation, the horticulture can be grouped into four major class such as Temperate, Sub-tropical, Tropical and Arid. India has become the second largest producer of both fruits and vegetables with a total production of 46. 75 million tonnes of fruits from an area of 4. 56 million ha and 93. 16 million tonnes of vegetables from 6. 30 million ha (2003-2004). Besides, there is a tremendous scope for increasing the production of flowers, spices and condiments, plantation crops and medicinal and aromatic plants. Floriculture has emerged as a viable agribusiness. The commercial cultivation of flowers in open field conditions was much popular than protected cultivation in India. But, now- a-days, the protected cultivation has brought a great revolution in Indian floriculture and it helps for the quality production and export to earn sizeable amount of foreign exchange. India has approximately 350 ha under green house cultivation with export value of approximately Rs. 330 crores during 2003-04(APEDA). On the other hand, the prevailing climatic conditions in India are highly suitable for cultivation of the almost all spices from tropical black pepper to temperate saffron. The major spices are mainly grown in humid tropics like Western Ghats and NE States. Out of 21 agro-ecological sub regions in India, spices are grown in 14 different agro ecological sub regions. Fruits, vegetables, flowers spices and condiments, plantation crops and medicinal plants are widely distributed across the country, while plantation crops are restricted to a few regions.

Introduction

Food and nutraceuticals are obtained from various agricultural resources available in this planet. Many types of bio-active compounds are available in small quantities in the plants and animals which are evidenced to improve performance of various organs, increase in immunity and cure for some diseases. Last decade evidences several advancements in developing phytochemical formulations which can be used as a cure for many diseases as well as helps in boosting immunity in human being. Such materials exist in different form, structure and chemical compositions. Examples of such chemicals are phenolic and organosulphur compound (Pascall 2011). The vegetables, fruits and seeds are used without further processing. Some plants are subjected to cooking before its consumption. Many industries are carried out extraction of bioactive compounds and developed products in the form of tablets, powders and liquids which are available in the market. In order to make commercial process more economic and feasible, long term storage of plant residues (Raw materials) as well as the prevention of the extracts emerges as essential requirement for the industries. Moreover, the products requires suitable packaging solution to increase its self-life, preventing it from the influences of environment, and to make viable solution for easy access which makes it more user-friendly. Suitable packaging solution is required for mainly the three reasons: (i) prevention. (ii) protection and (iii) presentation. Prevention is required in-order to avoid deterioration of the packaged items due to microbial growth, loss in moisture and enzymatic action. Protection is required for the purpose of protecting the packaging materials from the contamination as well as from the environment impact and rough handling. Presentation is equally important to provide it an aesthetic look to make it more approachable to the consumers. Moreover, packaging also offers “labelling for easy identification, warnings, instructions, and nutritional information, ease of transportation” (Pascall 2013).

The operation and maintenance of dehusking, dehulling, degerming, and drying equipment are crucial in ensuring the efficiency and longevity of these machines in grain and seed processing industries. These machines play a vital role in removing husks, hulls, and germs from grains, as well as reducing moisture content to enhance storage stability. Proper handling and regular maintenance not only improve performance but also reduce downtime and operational costs. Dehuskers are used to remove the outer husk from grains like paddy and millet. Their efficiency depends on factors such as roller pressure, feed rate, and machine alignment. Routine maintenance involves checking for wear and tear on rollers, adjusting pressure settings, and ensuring that all moving parts are well-lubricated. Similarly, dehullers remove the outer shell of seeds and grains, requiring careful calibration to avoid excessive grain breakage. Blade sharpness, motor performance, and sieve integrity should be regularly inspected to ensure optimal functioning. Degermers are employed to separate the germ from the endosperm in grains like maize. This process enhances the shelf life of processed products and improves their quality. Regular maintenance of degermers involves inspecting the abrasion plates, checking the feed rate, and ensuring that airflow mechanisms are clear of obstructions. Any malfunction can lead to uneven separation, affecting the final product quality. Dryers are essential in reducing the moisture content of grains to prevent microbial growth and spoilage. They require proper temperature control, uniform airflow, and regular cleaning of heat exchangers and drying chambers. Overheating or under-drying can compromise the grain quality and lead to storage losses. Timely inspections of sensors, air ducts, and burner efficiency help maintain effective drying operations. A well-planned operation and maintenance routine ensures these machines function optimally, increasing productivity and product quality while reducing energy consumption and repair costs.

Water is an essential component of all living things. Although once believed as a resource with a never-ending supply, it has now been understood that water is becoming increasingly scarce and therefore a proper management is necessary to keep a sustainable food production (Kannan & Anandhi, 2020). A continuous and safe supply of water is indispensable for the food industry where it has numerous applications along the agriculture and food production processes.

At present, food and nutrition security are the prime aspects which need to be addressed in an efficient way. It has been projected that by 2050 the global population will reach 9.2 billion which in turn enhances annual demand for agricultural products by 1.1 per cent from 2005 to 2050 (McKenzie and Williams 2015).

Introduction Millets are a group of annual grasses which are small seeded, hardy, drought tolerant, low maintenance and mainly found in semi-arid regions. The historic importance of millets can be derived from various types of traditional dishes which uses millets like roti and chapati flatbreads in India, uji (a type of porridge), injera (a fermented flatbread commonly eaten in Ethiopia) and beer in Africa, and kasha and fermented drinks in Eastern Europe. Millets are the 6th most important cereal grain in the world. India, China and Nigeria are the largest producers of millets, and the Research Institute for the Semi-Arid Tropics estimates that in Africa and Asia more than 90 million people depend on millets in their diets. There are hundreds of millet types, but the major types grown commercially are: proso millet, foxtail millet, pearl millet, Japanese barnyard millet and browntop millet. In this book chapter, we will mainly focus on two minor millets i.e., Teff and Fonio

5.1 Introduction

Food is the basic requirement of every living being and it provides physical, mental as well as emotional satisfaction. Globally, there are many food enterprises which provide this basic necessity across the nations irrespective of nature of origin and climatic condition. Food companies are required to provide products at certain quality parameters so as to maintain the uniformity in the food system. Hence, food quality can be defined as a total of traits and criteria which characterize food as regard to its nutritional value, sensory value, convenience as well as the safety for a consumer’s health. Food quality is the quality characteristics of food that is acceptable to consumers. This includes external factors as appearance (size, shape, colour, gloss and consistency), texture, and flavor; factors such as grade standards and internal (chemical, physical, microbial). Due to the various food risks, all over the world the quality regulation authorities have intensified the efforts to improve food quality. Some of the quality related systems are obligatory by law while other is voluntary which are to be implemented by the various food chain members.

Many companies are forced to improve safety and quality of their products by implementing the quality and safety assurance and management system because of growing consumers’ expectations and concerns as regards food quality and safety.

In order to attain maximum profit and decrease the flaws, a new system was developed and followed by various companies all over the world. This new system is known as Six Sigma. The application of this system has the capability to reduce the losses considerably by reviewing the process parameters and increasing the profit exponentially. It constitutes a systematic approach to assure that food products have particular traits at any stage of production and distribution.

Macaroni: Pasta made from semolina and shaped in the form of slender tubes (Plate 396).

Macaroni wheat: It is tetraploid wheat and is preferred for making macaroni, vermicelli etc.

Macaroon: A small cookie composed chiefly of egg whites, sugar, and ground almonds or coconut (Plate 397).

Mace: An aromatic spice consisting of the dried external fibrous covering of a nutmeg (Plate 398).

Mach number: It is a number representing the ratio of the speed of a body (flow velocity) to the speed of sound (sonic velocity) in a surrounding medium (as air).

Macroglobulin: A highly polymerized globulin of high molecular weight.

Macronutrient: A chemical element (as nitrogen, phosphorus, or potassium) of which relatively large quantities are essential to the growth and health of a plant or a substance (as protein or carbohydrate) essential in large amounts to the growth and health of an animal.

8.1 Introduction

Electron Microscopes were developed due to the limitations of Light Microscopes which are limited by the physics of light to 500x or 1000x magnification and a resolution of 0.2 micrometers. In the early 1930’s this theoretical limit had been reached and there was a scientific desire to see the fine details of the interior structures of organic cells (nucleus, mitochondria etc.). This required 10,000x plus magnification which was just not possible using Light Microscopes. The Transmission Electron Microscope (TEM) was the first type of Electron Microscope to be developed and is patterned exactly on the Light Transmission Microscope except that a focused beam of electrons is used instead of light to see through the specimen. It was developed by Max Knoll and Ernst Ruska in Germany in 1931. The first Scanning Electron Microscope (SEM) debuted in 1942 with the first commercial instruments around 1965. Its late development was due to the electronics involved in scanning the beam of electrons across the sample. Electron microscope has been a valuable tool in the development of scientific theory and it contributed greatly to biology, medicine and material sciences. This wide spread use of electron microscopes is based on the fact that they permit the observation and characterization of materials on a nanometer (nm) to micrometer (µm) scale. Electron Microscopes are scientific instruments that use a beam of highly energetic electrons to examine objects on a very fine scale. This examination can yield information about the topography (surface features of an object), morphology (shape and size of the particles making up the object), composition (the elements and compounds that the object is composed of and the relative amounts of them) and crystallographic information (how the atoms are arranged in the object). A scanning electron microscope (SEM) is a type of electron microscope that produces images of a sample by scanning it with a focused beam of electrons. The electrons interact with atoms in the sample, producing various signals that can be detected and that contain information about the sample surface topography and composition. SEM can achieve resolution better than 1 nanometer. The combination of higher magnification, larger depth of focus, greater resolution, and ease of sample observation makes the SEM one of the most heavily used instruments in research areas today.

According to Pasteur statements which one of the following is true

a) Living organisms discriminate between stereo isomers

b) Fermentation

c) Living organisms doesnít discriminate

d) Both a and b

4.1 Introduction

A huge amount of waste in the form of liquid and solid is produced in the fruit and vegetable processing industry which causes pollution problem if not utilized or disposed off properly. Generally solid waste is dumped in local landfills, eaten and scattered by stray animals, which has now become a major cause of environmental pollution. The waste obtained from fruit processing industry is extremely diverse due to the use of wide variety of fruits and vegetables, the broad range of processes and the multiplicity of the product [79]. The solid waste from fruits and vegetables includes peel, stone, rag, seed, core etc is of economic value and can be used for manufacturing of various by-products [21]. The extent of the waste produced from processing industries of some of the important fruits and vegetables, is given in Table 1.

In India, utilization of fruits and vegetables for processing is hardly 1.8% of the total production. There were 5166 processing units till 1^st Jan, 2009 of fruits and vegetables products throughout the country. About 90% of these units are small scale units with annual production limit of 50-250 tonnes. These units have no organized methods of waste disposal. In certain places this waste is taken away by farmers for their disposal as cattle feed or field manure.

• The proportion of bone varies depending on breed and body condition from 12 to 28% in the dressed carcase and approximately 15% in a good large ruminants carcase
• Bone percentage increases with the age and weight of the animal and it is lowest in Aberdeen Angus cattle and it may increase to 28% in low quality large ruminants
• The average bone % in bobby calves is 50%, veal calves 25%, in lamb 17–35%, in pork 12–20% and in poultry 8–17% Important parts of digestive system of meat animals Ox tongue
• Filiform papillae found on the tongue are pointing backward and horny and thus, helps in food prehension
• Actinobacillosis erodes the prominent posterior part of the tongue dorsum
• 10–14 circumvallate papillae are spread in the region of prominent dorsum around the either side of the tongue midline
• Black pigmentation of the tongue surface is fairly normal and not associated with any disease condition
Sheep and goat tongue
• Similar to cattle tongue
• However, papillae are not horny but the centre of the tip is slightly grooved
• Black pigmentation of the surface of the tongue is common in blackskinned sheep.

Introduction
• Slaughtering of meat animals in way so as to effect minimum pain and suffering to animals is referred as humane slaughter
• Slaughtering method followed should inflict least stress and suffering to the animals
• There are acceptable and the unacceptable slaughtering methods and there is no ‘nice’ method to slaughter the food animals
• In advanced countries, common slaughtering method consists of two steps, stunning and bleeding
• Stunning is done to make animal unconscious and not to feel the pain during actual bleeding process which is followed next
• Exception of stunning-- Jewish and Muslim methods of slaughter
Stunning
Humane slaughter consists of stunning followed by sticking (bleeding). Stunning is the process of making the animal unconscious till it is slaughtered so that the pain suffered during actual act of slaughtering can be alleviated