eChapters

Microorganism is a collective term for all living organisms, which are not visible to the eye, and occupy an intermediate position between the vegetable and animal kingdoms. They are found every where in the atmosphere, in water and in the soil and, as they break down organic materials, they play a very important role in the cycle of the nature.

There are thousands of species which are important to the life and economic structure of the human race. For example, certain species form simple chemical elements during the break down of dead organic material which plants can use again. They increase soil fertility and crop production which results in more food.

Other species are present, and essential, in animal intestines, helping in the digestion of food.

Some microorganisms are used in many food processes, e.g. dahi, yoghurt, cheese, pickles, beer and wine as well as in the acid production to preserve foods. There are microorganisms that produce toxic substances which kill other organisms, e.g. the mould penicillium which produces the substance penicillin.

Many microorganisms cause diseases in animals and plants with a subsequent reduction in the nation’s food supply, whereas others cause food deterioration such as mouldy foods, tainted foods, etc.

10.1 Introduction

Milk is a unique substance in that it is both consumed, as fluid milk with minimal processing and it is the raw material used to manufacture a wide variety of products. From the nutrition point of view, milk is an almost ideal food and considered to be perfect food. The human body needs more than thirty distinct materials in its food supply. No single food stuff supplies all of them but milk supplies nearly all. Since milk by its nature is perishable and an ideal product for bacterial growth, so it must be carefully handled, both during production and while processing. This chapter focuses on the technological (processing) aspects of the transformation of milk into its various consumer products, including beverages, fermented products, concentrated and dried products, butter and ice cream.

Leafy vegetables are rich sources of provitamin-A, vitamin C and minerals like calcium, magnesium, iron, etc., They are the riches sources of roughes or fibres essential in human diet. The ICMR recommended dietary allowances of green leafy vegetables for adult women is 100g/day and men are 40g/day while for school children above 10 years it is 50g/day. Greens are rich in vitamins and minerals essential for healthy functioning of organs of body. Amaranth, beet leaf, spinach, fenugreek, etc., are the major leaf vegetables grown in India. In addition, a number of under-utilized annual crops are also grown as leaf vegetables in specific regions. Tender stems and leaves of a number of perennial crops are rich sources of vitamins and minerals and are used for cooking.

10.1. Amaranth (Amaranthus sp)

Amaranth is the most common leaf vegetable grown in India. Amarnath is an annual herb with erect growth and scarce to profusely branching habit. Stem is succulent and green or purple or mixed shades of these two. There are two sections in Amaranthus, viz., Amaranthus and Blitopsis. Majority of leaf cultivars grown in India belong to Amaranthus tricolor. Among the several kinds of greens Amaranthus occupy a special place because it has a large number of species grown in one or the other part of country.

Some of the important improved varieties released are CO-2 and CO-5 from TNAU, Coimbatore, Pusa Kiran, Pusa Kirti and Pusa Lal Choulia from IARI, New Delhi, Arka Arunima and Arka Suguna from IIHR, Bangalore. Amaranth are popular all over India by different names. While in northern parts it is popularly known as choulia, in south it is popularly called as keerai or cheera or koora or soppa. The general composition of amaranth is given below:

1.    Introduction
    
2.    Structure of Protein
    2.1     Primary structure
    2.2     Secondary structure
    2.3     Turns and Bends in the Polypeptide Backbone
    2.4     Tertiary structure
    2.5     Quaternary structure
    
3.    Protein Denaturation
    
4.    Conditions Affecting Protein Structure
    
5.    Fish Proteins
    5.1    Sarcoplasmic proteins
    5.2    Myofbrillar protein
    5.3    Stroma proteins
    5.4    Functional properties of fish proteins
    
References
    
Further reading

1.    Introduction
    
2.    Different Forms of Value Added Products
    
3.    Production of Fish Steaks
    
4.    Battered and Breaded Products
    
5.    Minced Fish Based Products
    5.1    Fish cakes
    5.2    Fish cutlets
    5.3    Fish sausage
    5.4    Dehydrated salted fish cakes
    5.5    Fish fingers
    5.6    Fish burgers
    5.7    Fish balls
    5.8    Fish wafers
    5.9    Fish soup powder
    
6.    Surimi and Surimi Based Products
    6.1    Kamaboko
    6.2    Chikuwa
    6.3    Tempura
    6.4    Hampen
    
7.    Modern Seafood Analog Products
    7.1    Imitation crab meat
    
8.    Marinated Fish Products
    
9.    Fermented Fish Products
    9.1    Fish pickle
    
10.    Sous Vide Cook-Chill Products
    
11.    Precooked Seafood Entrees
    
12.    Future Needs
    
References

Abattoir: An establishment where animals are butchered is known as abattoir. It is also known as slaughterhouse.

Abiotic: Abiotic means any factor which is not biological / a biological and especially there is no involvement of the organisms in synthesis of certain compounds.

 Abnormal milk: It is milk that is not smooth and homogenous but is flaky, ropy, stringy, slimy, water, discoloured or bad flavoured. It may be due to animal sickness or disease or due to excessive medicines given or uncleaned milk condition.

 Abomasum: It is the fourth stomach of the Ruminants which is lying close to the

Omasum or the third stomach, in which digestion gets completed by the supply of the digestive juice. It is also called Reed stomach, rennet stomach, rennet-bag, or reed

tripe and maw (Plate 1).

 Abrassive peeler: It is equipment comprising a rotating drum lined with carborandum (an abrasive material) in which certain vegetables like potato and ginger are tumbled and exposed to the abrasive action of the carborandum lining, thereby facilitating separation of peel from the vegetables (Plate 2).

2.1 Introduction

Food is one of the complex systems involving many constituents such as carbohydrates, lipids, proteins, vitamins, minerals and other phytochemicals. These all constituents are not present in the same relative proportion in every type of food and thus the foods are categorized and valued according to the presence of these constituents as protein rich foods, fat rich foods, etc. The specific type of food is in abundance with only one or at most two of these constituents and rest of the bulk is not valuable, commercially. So, if these active constituents which are present in the tissue surrounding inactive matrix, are separated, it is of great benefit with respect to increased purity, nutritive value, functional properties, reduction in volume, easy handling and transportation. On contrary some valuable food items contain undesirable constituents generally referred as anti-nutritional factors. Removal of these anti-nutritional factors enhances the food value and commercial utilization. Hence, the separation of phytochemicals and removal of anti-nutrients from the food matrices can be easily carried out by using a unit operation called as extraction.

Extraction involves the separation or isolation of active/desirable components of complex system viz., plant and animal tissues from the rest of inactive/undesirable components by using suitable means such as solvent or mechanical structure. The basic principle involved in the extraction process is partitioning of different components in accordance with the extracting agent. For example, in mechanical extraction, the partitioning is due to pressure and screen/apperature size where as in case of solvent extraction, the portioning of components from mixture takes place due to particular solubilizing power of solvent used.

1.1 Introduction

Due to the ever increasing world population, the demand for food would also increase. India is one of the largest food producer countries of the world (first in milk production, second largest in fruit and vegetable production and third largest in grains production). It is the seventh-largest country in the world, with a total land area of 3,287,263 square kilometres. India has varied climates of snow covered Himalayas, desserts, oceans, fertile plains and areas receiving the highest rainfall in the world. Name any food item; it can be grown in one or the other part of the country. All these make the production of various types of foods of plant (fruits, vegetables, cereals, pulses, oilseeds, spices etc) as well as animal origin (fish, meat, milk etc.) possible in the country. The annual production of cereals, fruits and vegetables is 260, 57 and 77 million tonnes respectively, besides this, the production of cow and buffalo milk is about 102.1 million tonnes and that of sugarcane and spices are 355.52 and 1.1 million tonnes respectively [15]. Table 1 shows that the production of various food items has increased many folds during the last five decades. On one hand, we feel proud as we produce one of the largest quantities of foods in the world and there has been an enormous rise in the production of various foods in India during the last five decades, on the other hand we should be very much worried being a leading country as far as the wastage of food in the world is concerned. The postharvest losses are huge and only meagre quantity (2%) of the food is processed. Sharma [41] very rightly said that when we do not agree to throw away Rs 4000/-per month out of a salary of Rs 10,000/-per month then why do we accept 40% postproduction losses of food in the country. This would require immediate necessary action to save a major portion of the food that has already been produced.

Who discovered bacteria?

a) Leeuwen hock b) Ledge berg

c) Walkman and Zinder d) None

India is the second largest producer of fruits However, the export potential of fruits and vegetables is very negligible. This is because of improper knowledge to the farmer about proper post-harvest infrastructure facilities. Fruits have a high-water content ranging from 70-95 per cent. They dry quickly under any normal atmospheric condition. These causes loss of rigidity and shrinkage of the cells cause wilting and shrivelling. Appropriate packaging ensures prolonged the shelf life of fruits and vegetables by preventing moisture loss and thereby wilting. Transportation is a critical phase in the fruit supply chain that can significantly affect the quality of fruits. Several factors during transportation can lead to quality degradation such as temperature/humidity fluctuations, mechanical damage, transit time etc. To mitigate the adverse effects of transportation on fruit quality, best practices ie, real-time monitoring, temperature and humidity control, loading/unloading techniques can be employed. Quality evaluation is a critical component in the fruit industry, encompassing a range of assessments to ensure fruits meet the desired standards for consumer satisfaction, shelf life, regulatory compliance, and market competitiveness. It involves a comprehensive approach that includes physical, chemical, and sensory assessments, supported by advanced technological tools, to maintain and enhance the quality of fruits throughout the supply chain. This book chapter deals with the effective packaging, transportation, and quality evaluation of fruits, ensuring that consumers receive fresh, high-quality produce.

A. Non-thermal Processing

Non-thermal processing encompasses a diverse range of food preservation and treatment techniques that do not rely on heat as the primary means of achieving microbial inactivation or quality preservation. Unlike conventional thermal methods such as pasteurization and sterilization, which involve exposing food to elevated temperatures for a specified duration, non-thermal processing methods operate at or near ambient temperatures. These innovative technologies offer several advantages, including the preservation of sensory attributes, retention of nutritional quality, and minimization of detrimental effects on food texture and f lavour (fig 1).

1. The Stability and Shelf-Life of Food

    1.    In the UK, the date coding to be used is determined by the total life of the product: for microbiologically highly perishable foods, a ‘use by’ date is needed, while for other foods, including foods with more than 18 months’ shelf-life, a ‘best before’ or a ‘best before end’ date is needed.

    2.    IFT (1974) definition of shelf-life, is : The period between manufacture and retail purchase of a food product during which the product is of satisfactory quality.

    3.    Shelf-life is defined as the time during which the food product will :

        i.    remain safe;
        ii.    be certain to retain desired sensory, chemical, physical and 
    microbiological characteristics;
        iii.    comply with any label declaration of nutritional data,

Introduction

For the year 2016-17, as per the FAO global estimate, the food grain production has crossed over 2.6 trillion metric tons, while the steadily increasing the demand is anticipated to be approximately 3.3 trillion metric tons for 2016-17. Similarly,the global production of all horticultural commodities has crossed over 1.8 trillion metric tons. The global trade of these commodities is purely based on their quality, the demand for good quality produces increases day by day. The term “quality” is a broad terminology used to explain many parameters and differs from commodity to commodity. Usually, the term ‘quality’ is classified into internal and external quality. The external quality refers the external colour, size, shape appearance, uniformity, external damage, cracks and bruishness etc. These parameters may be common for both perishables and durables.

But, in case of internal quality, each and every parameter vary from commodity to commodity, product to product. For food grains, the internal quality refers to moisture content and other nutritional parameters like carbohydrate, protein content etc. But in fruits, the internal quality refers to internal pulp colour, TSS, pH, acidity, juice content, ripeness, other biochemical and nutritional quality parameters. 

Introduction

Seafood undergoes rapid spoilage after capture or harvest due to their highly perishable nature. Spoilage refers to any change that renders seafood unacceptable for human consumption. Spoilage occurs as a consequence of various microbial, biochemical and chemical breakdown processes. Spoilage leads to quality changes in seafood. Quality change refers to any change that occur after harvest due to unpredictable catch, uncontrolled harvest, inadequate handling, processing and storage facilities. The initial quality loss is mainly due to the post-mortem autolytic activity (enzymatic process) and chemical degradation process (lipid oxidation); and later by microbial degradation. The quality problems occur as a result of poor-quality raw material, ice and water, microbiological contamination, presence of heavy metals and other storage aspects during processing.

Processing of fish means preparing raw fish into a marketable form. Pre processing is done prior to final processing. Pre-processing is often done on board fishing vessel or in a shore based plant. It includes operation such as washing, sorting, grading, butchering, etc of the harvested fish. The butchering involves removal of non-edible portions such as viscera, head, tails and fins from fish. The yield of fish meat varies from 30 to 70% depending on the butchering process. Butchering process includes beheading, gutting, filleting, peeling, deveining, shucking, etc. The discarded portion of fish meat are fish wastes, often used for animal feed or for conversion to bioactive functional foods. Final processing of fish includes processes such as heating, freezing, drying, salting, marinading, irradiation, or addition of chemicals. The processed seafood is categorized into five major groups – chilled, frozen, canned, cured and other value-added products in this chapter.

Introduction

The food that we consume should not only contain all essential nutrients like carbohydrate, protein, fat, vitamins, minerals, fibre etc but also should be hygienic and safe. Safety and quality of food is to be ensured not only during food production but also should be continuously monitored till it reaches the consumer (Coles and Frewer, 2013). The characteristics of food are altered during every processing operations viz., cleaning, sorting, transportation, thermal processing, packaging, storage, distribution etc which may be desirable or undesirable. Persistent physical, chemical and microbiological analyses have to be done at all levels of supply chain to ensure the quality of food. Traditional methods of food safety analysis are spectrophotometry, aerobic plate count method, microscopic plate count, titrations, chromatography etc. Though these techniques provide accurate results, the analysis demand cumbersome extraction steps, trained technical staff and are time consuming (Di-Natale et al., 2000). Hence, steady, sensitive and rapid monitoring of food analysis technique is a need of the hour in the present era. Biosensors can effectively replace the conventional techniques owing to its reliability, rapidity, economic and field applicability (Prasad et al., 2014). Biosensors are an attractive diagnostic alternative owing to their in situ detection ability and comparatively reduced detection time from several days to hours, or even minutes (Ruiz et al., 2017). Rapid food analysis can also minimise spoilage, especially in freshly harvested agricultural commodities, such as fruits and vegetables, meat, fish etc. In addition, the portable detection devices provide in-situ detection capabilities without extensive training and assist in integrating real time analysis in food processing centres.

Introduction
Chapter 11 delves into the intricate relationship between food sciences, human nutrition, and their collective impact on food security and global sustainability. The science of sustenance, encompassing food processing, preservation, quality, safety, and nutrition, is crucial for understanding how to feed a growing global population while minimizing environmental impact. The chapter begins by exploring food processing and preservation techniques, which are essential for extending the shelf life of food products and preventing food wastage. Traditional and modern food processing methods are discussed, highlighting their role in maintaining food quality and safety. The importance of food preservation is emphasized, along with emerging trends in food technology that address current and future challenges. Food quality and safety are vital components of the food sciences, ensuring that the food consumed by populations is not only nutritious but also free from harmful contaminants. The chapter examines the factors influencing food quality, including environmental conditions, handling practices, and storage methods. The principles of food safety are outlined, addressing the critical need for stringent measures to prevent foodborne illnesses. The challenges in maintaining food safety in the face of global trade and climate change are also discussed. Nutritional requirements and their role in human health are covered in the next section. This part of the chapter introduces fundamental concepts of nutrition, such as macronutrients, micronutrients, and dietary guidelines that promote health and well-being. The importance of balanced diets is underscored, with an emphasis on the need for proper dietary assessment methods to ensure adequate nutrient intake. The concept of food security is then explored, considering the various factors that influence the availability, accessibility, and affordability of food. The chapter discusses strategies for enhancing food security, including improving agricultural practices, reducing food waste, and addressing socio-economic inequalities. The integration of food sciences and human nutrition is presented as a critical approach to achieving sustainable food security. The chapter also examines the impact of food sciences and human nutrition on global warming. Food production, as a significant source of greenhouse gas emissions, is analyzed with respect to its environmental footprint. Strategies for efficient resource utilization, policy advocacy, and research innovation are

Introduction

Oils and fats are the major class of compounds in lipids. Oils and fats are essential nutrients in both human and animal diets and produced from plant, animal, marine and microbial sources. Dietary oils and fats are concentrated sources of metabolizable energy (9 kCal/gram) compared to protein or carbohydrates (4 kCal/g). According to the recommendations of Food and Agriculture Organization (FAO) of the United Nations, the maximum total fat intake for adults is 30 to 35% E (total energy calories). The minimum fat intake recommended is 15% E for most individuals and 20% E for women of reproductive age and for malnourished populations (FAO, 2010).The current dietary guidelines for optimal health and prevention of diet-related chronic diseases like diabetes, coronary heart disease (CHD), cancer and obesity emphasize the importance of both the quantity and quality of total fat in the diet. In addition to the caloric and nutritional value, oils and fats exhibit many functional properties in the diet. Oils and fats enhance the value of foods by releasing the flavours of other food components, provide texture, increase palatability of food, and impart the feeling of satiety after eating. Oils and fats provide essential fatty acids (omega 3 and 6) which are the building blocks for several metabolic pathways for the healthy life. Oils and fats are excellent carriers of fat-soluble vitamins A, D, E and K, and the absorption of these vitamins is impaired at very low-fat intakes. Due to this reason, sseveral countries have started using edible oils and fats as a suitable vehicle for the fortification of vitamin A and D as edible oils are consumed by almost everyone (Diosady and Krishnaswamy, 2018). Oils and fats are structural bodily components, and are involved in vital physiological processes, including growth, development, inflammation, and brain function. Lipoproteins are important cellular constituents, occurring both in the cell membrane and mitochondria, and also serving as the means of transporting lipids in the blood. Oils and fats are responsible for making biological membranes such as phospholipids and cholesterol, vital for making the cell membranes. Most of the edible oils contain several minor constituents which are known as potent nutraceutical constituents with several health benefits. In addition to edible purpose, oils and fats are being used in many industrial applications such as soaps, detergents, cosmetics, lubricants, specialty chemicals, biofuels, etc. Hence oils and fats are indispensable source for food (edible oils and nutraceuticals), fuel (biodiesel, lubricants etc.) and fabulous chemicals (oleochemicals).

Pineapple is a tropical plant with edible multiple fruit consisting of coalesced berries. It is named after pine cone due its resemblance with its structure. It is the most economically important plant in the family of Bromeliaceae. It is widely grown throughout the world for its dual-purpose use in the form of fresh fruit and many processed products. It is a tropical fruit having excellent taste, juiciness, aroma and flavour which is preferred by many people. It is also rich source of fibre, potassium and vitamin C, vitamin B1 and vitamin B6. One of the important constituents in the fruit is proteolytic enzyme bromelein which is known to have significant use in treating conditions like inflammation, sinusitis, arthritis and early recovery from surgery (Joy, 2010). Bromelein is a digestive enzyme also used as meat tenderizer. Pineapple is cultivated in an area of 106.22 thousand ha and total production is 1,527.93 thousand tons (APEDA). Major producing states of pineapple were West Bengal, Assam and Karnataka. Pineapple is highly perishable fruit which transpires heavily and lose moisture thus have high susceptibility to spoilage microorganism (Hossain and Bepary, 2015). There are often several difficulties in production of pineapple with its physiologically active state even after harvest and non climacteric nature. Even though production is more, there are enormous post harvest losses for the fruit. The overall losses being encountered constitute 32.12 percent (6.82% at farm level, 3.09% at wholesale market and transportation, 5.44 % at retail market level, 5.64% during storage, 4.31% in processing unit and 6.82% at consumer level respectively) as reported by Ningobam, (2019).

Introduction

Fruits and vegetables (F&V) are good sources of phytochemicals like carotenoids, phenolics, dietary fibers, minerals and vitamins and are considered as important components of a healthy diet. These bioactive compounds in fresh F&V have been linked to delay in onset of many diseases including cardiovascular, chronic and other non-communicable diseases. WHO reported an approximate 3.9 million deaths all over the world in 2017 due to insufficient consumption of F&V (WHO, 2019). Hence, health advisors and nutritionists recommend inclusion of fresh fruits and vegetables to complement the daily diet, and WHO recommends the consumption of a minimum of 400g F&V daily in five portions. However, beneficial raw F&V may contain pathogenic microorganisms, such as Salmonella, E. coli, and Listeria that can pose threat to consumer safety. Since fruits and some of the vegetables are eaten raw, there are chances that pathogenic microorganisms may also enter food chain. Nearly a half of food borne illnesses in United States are caused by microbes on fresh produce (CDC, 2019). The F&V are handled by producers, dealers, wholesalers, retailers and several intermediaries, and therefore, are at greater risk of possible contamination till it reaches the consumer.

The ever increasing demand for F&V has led to overexploitation of pesticides usage in the farms, leading to harmful pesticide residues in air, soil, water and on the F&V surfaces. Thus, it is imperative to maintain the quality and safety of F&V all through the supply chain. In this chapter, we discuss various pre and postharvest factors affecting the quality and safety of F&V, storage techniques to extend shelf life and reduce the safety threat as well as methods available for microbial and pesticide decontamination of fresh F&V.

3.1. Introduction

Paper and paper-based packaging is the traditional form of packaging used for various food products due to its various advantageous characteristics. It is less expensive, flexible, lightweight, durable, recyclable and available in various shapes, sizes and colour to meet customer needs. Hence it occupies a vast market in food packaging from primary (i.e., packaging which is first used after manufacture) to secondary (i.e. processing aids of primary packages) packaging and the production of paper has doubled in the last three decades. In the year 2020, china was the leading producer for paper and paper based packaging followed by USA and Japan (Figure 1).