eChapters

ABSTRACT

Owing to paucity of available genetic / molecular markers, onion breeding still relies heavily on phenotypic selection methods. DNA markers can provide plant breeders genetic information for indirect selection of plants. All the DNA based procedures require high quality plant genomic DNA. Abundance of polysaccharides and phenolics present in plant tissue often impede PCR amplification. The polysaccharide contaminants can also produce downstream problems by inhibiting many of the enzymes that are employed in subsequent analysis and cloning. RFLP based markers in Allium species pose certain problems due to relatively large size of the genome compared to most other herbaceous crops. As an alternative, RAPD would prove advantageous in genetic analysis of Allium. Here we present our results on optimization of DNA extraction in onion to get amplifiable quality of DNA for further RAPD analysis. Leaf samples were collected from the onion germplasm maintained at Indian Institute of Horticultural Research (IIHR), Bangalore. Dried leaf protocol was suitably modified to obtain fair quality of DNA. After varying each of the ingredients of the extraction buffer we arrived at the optimal extraction buffer, which resulted in good quality DNA. Quality was analyzed by subjecting the DNA to agarose gel electrophoresis and restriction digestion. Extracted genomic DNA was quantified with ‘Hoefer’s Dynaquant’ (Pharmacia, USA). The DNA quantity ranged between 160-2400mg/gm of dried powder. The standardized 25ml PCR mixture contained template DNA (25ng), 5 pmol of primer (Operon Tech. Inc., Alameda), 2mM MgCl₂, I unit of Taq polymerase (Bangalore Genei), with 10x buffer along with 200 mM each of dNTPs. Amplifications were performed in thermal cycler (MJ Research, PTC 100) for 45 cycles after an initial denaturation of 95°C for 3 minutes. In each cycle denaturation for one minute at 95°C, annealing for one minute at 35°C and extension for two minute at 72°C was programmed with final extension step at 72°C for 5 minutes. This fine tuned protocol resulted in clear, informative RAPD profile.

Extension worker play a important role in rabbit keeping. It increases food production and experience in rabbit farming, people has to be educated as they have no knowledge of rabbit keeping especially people of towns and villages.

These have to be told the advantages of rabbitry. It is difficult to convince villagers, so extension is very important.

Rabbit Census : Recently taken up.

In Karnataka, census was taken in 1990. There were 16,327 animals.

The presence of a Y or X chromosome influences sexual differentiation and the development of reproductive organs. The reproductive system comprises the gonads, genital ducts, external genitalia, and gonad descent. Development of reproductive system is initiated in gonads which differentiate into ovary or testis. The formation of genital ducts and glands occur later. Differentiation of reproductive system is a complex process which involves a number of different mechanisms operating at several stages of development. It occurs only after the arrival of primordial germ cells. First, the gonads undergo an indifferent stage before differentiating into the testes and ovaries. Thereafter, the sexual differentiation of the genital ducts is determined by the presence or absence of testosterone and Anti-Müllerian hormone, both of which influence the outcome of the mesonephric and paramesonephric ducts. Then sexual distinction of the external genitalia is also determined by hormonal secretions, or lack thereof and finally, the gonads descend primarily due to the shortening of the gubernaculum. Importantly, the development of the reproductive system is directly related to that of the urinary system. Both systems develop from common intermediate mesoderm and their excretory ducts terminate in a common cavity known as cloaca) Two important ducts develop, i.e. wolffian duct (in males) and mullerian duct (in females). Mesonephric duct which is also known as Wolffian duct, originates from pronephric duct. Pronephric duct continues to grow caudally till it opens into the ventral part of the cloaca. Beyond pronephros (primitive kidney), it is called mesonephric duct. In males, this duct persists and forms epididymis, vas deferens and seminal vesicle. In females, this duct undergoes atrophy. Shortly after the formation of Wolffian duct, a 2nd pair of duct develops i.e. Mullerian duct. Each arises on the lateral aspect of the corresponding Wolffian duct as a tubular invagination of the cells lining the abdominal cavity. Mullerian duct opens on cloaca between the orifices of Wolffian ducts. In females, mullerian duct persist and undergo further development. Gonads develops from 3 important sources, namely mesothelium lining posterior abdominal wall, underlying mesenchyme (Embryonic CT) and primordial Germ Cells

Development of fungicide resistance in a fungal disease pathogen is a major concern for some of the important diseases in their management. This can be overcome by designing of fungicide use strategies to manage the threat of fungicide resistance.

Introduction
The development of the respiratory system in animals is a crucial process that begins early during embryogenesis. The respiratory system, responsible for gas exchange, develops from the endoderm of the early embryo, which is one of the three germ layers. Specifically, the respiratory tract develops as an outgrowth from the primitive gut tube (foregut). This process involves complex interactions of molecular signalling, tissue differentiation, and organ morphogenesis.
Key Stages of Respiratory System Development
1. Formation of the Respiratory Primordium
The respiratory system originates from the laryngotracheal groove, which appears on the ventral wall of the primitive foregut. This groove eventually forms the laryngotracheal tube, which serves as the foundation for the respiratory tract.
2. Division of the Foregut
The foregut is divided into two regions: The dorsal portion (oesophagus) for the digestive system. The ventral portion (laryngotracheal tube) for the respiratory system. This separation is achieved by the formation of the tracheoesophageal septum.
3. Development of the Trachea and Lung Buds
The laryngotracheal tube elongates and gives rise to the trachea) At its distal end, two lung buds form, which will develop into the bronchi and lungs. These lung buds expand, branch, and grow into the surrounding mesenchyme, a process called branching morphogenesis.

Objective: Development of RTS beverage from seasonal fruits.
Material required
Fruit, juicer-mixer-grinder, preservatives, sugar, refractometer, pan and knife etc.
Principle
Ready To Serve(RTS): This a type of fruit beverage which contain at least 10% fruit juice and 10% total soluble solid besides about 0.3% acid. This is not diluted before serving.
Before undertaking the preparation of beverage, it is necessary to know techniques of extraction of pulp/juice from various fruits used for RTS.

Procedure
1. Mix the dough with ingredients I. Keep it for fermentation till breaks easily without stretching.
2. Put the sponge to mixer. Add salt, sugar, flour and optional ingredients and mix. In last, add shortening. The dough should be soft and very smooth.
3. Let the dough relax for about 30 min. cut pieces of dough weighing 100 g/450g.
4. Mould in along oblong shape and place in baking pans.
5. Proof the moulded dough to a desired height.
6. Bake at 210oC to a golden colour.
7. Cool the loaves overnight.
8. Cut the rusk of desired thickness. Arrange on baking sheets and toast at 190oC till these are crisp and golden brown in colour.
9. Cool and packaged.

Objective: Development of Salted Banana chips.
Banana is the second most significant natural product harvest of India close to mango. It is accessible round the year and due as it would prefer, nutritive and the rape tic worth. It is most loved product of all classes of individuals. It is a rich wellspring of sugar and nutrients especially nutrient B. Banana assists with diminishing the danger of heart illnesses. Banana is a famous organic product because of its low cost and high nutritive worth. Banana contains about 20% sugar and sensible measure of Vitamins A, B and C. This is considered to be a rich source producing food. It is consumed in several forms and among many worth added arrangements, Banana Chips is viewed as the main thing. Seared banana chips are typically delivered from under-ready banana cuts southern style in sunflower oil or coconut oil. These chips are dry (like potato chips), contain about 4% water (table), and can be salted, spiced, glossed over or jaggery covered. Some of the time banana enhancing is added.

Introduction

Since shrimp aquaculture became a big commercial enterprise in the 1970s, disease has had a significant influence on the industry. Major diseases of farmed shrimp have been identified as those caused by viruses, true bacteria, protozoa, and fungi. Nowadays, chemotherapeutics, regular sanitization, and enhanced culture procedures are employed to treat many bacterial, fungal, and protozoan-caused illnesses. Shrimp farming continues to be impacted by diseases, and their avoidance necessitates the development and use of a biosecurity policy unique to each facility, culture system, and sanitary zone.

Objective: Development of Sugarcane Vinegar.
Principle: Sugarcane is one of the main crops worldwide, and it has an important impact on environmental issues. Sugarcane is used in daily routine life in many ways like as vinegar, jiggery, juices etc. In India sugarcane crop is best cultivated in the west U.P. Sugarcane original vinegar drink with high nutritional quality was produced from fresh sugarcane juice using the yeast culture and acetic acid bacteria by fermentation techniques such as submerged alcoholic fermentation followed by acetic fermentation at room temperature. Refined sugarcane is the primary product of sugarcane juice, during its processing; various other valuable products are also obtained in an unrefined form such as brown sugar, molasses, jaggery and vinegar. Sugarcane juice is widely used in the treatment of jaundice, hemorrhage, dysuria, and other urinary disease. Nowadays sugarcane vinegar is also used in Indian kitchens commonly in pickles, salads, etc. Vinegar is extremely useful for human health including antimicrobial activity, blood pressure reduction, antioxidant activity, reduction in the effects of diabetes, and prevention of cardiovascular disease. Vinegar can be made from any carbohydrate source, amylaceous, or sugary substrate through two successive fermentations: alcoholic fermentation, which is carried out by means of yeasts, and acetic fermentation, with acetic bacteria as the protagonist.

1. INTRODUCTION

India is blessed with all types of vegetation because of the diversified climatic and physiographic factors like tropical, subtropical, temperate and alpine. Due to its wide ranging environmental regimes and diverse biological communities, it is one of the world’s 12 “Mega diversity” nations. Out of nearly 425 families of flowering plants in the world, 328 families with 21,000 species are found in India. Further, in India about 20.64 % area is under forest where various species of trees are found, depending upon the climatic conditions.

There are about 29 species of pine which produce edible nuts and utilized at least by indigenous tribal culture in the world. Most of these nut bearing pines are haploxilon (soft) pines, although several species are of diploxilon (hard) pine group. Spain is the largest producer of Pinus pinea and China is the largest producer and exporter of Pinus koriences and Pinus sibrica nuts. Some of the famous edible pine nut species are listed in Table 1. Chilgoza (P. gerardiana Wall.), commonly known as ‘chilgoza’ or ‘Neoza’ is a member of genus Pinus that maintains a very unique position of being an integral part of the vibrant, but fragile ecosystems of the great Himalayas in the Indian sub-continent. The chilgoza pine is the only pine which is of immense social forestry importance. The earning for the bread and butter of most of tribals in HP is from the chilgoza nuts. Further, it also forms an important part of their diet as well as an item essential for some social obligations. Tribals are still adopting the age old practices for chilgoza nut extraction and further their selling to the agents at throw away prices during peak season of nuts.

Abstract

There are enormous food processing industries comprising of grains and oilseeds milling, fruits and vegetables processing, spice grinding and other food processing units in India. Also, there are many seed processing companies in the country, which are engaged in the processing and production of good quality seeds of different crops besides National Seeds Corporation and State Seed Corporations. All industries are using different type of machinery and equipment like cleaner, grader, expeller, milling machines, polishers, dehusking machines, size reduction equipment, different type of grinders, separators, treatment machinery, mixers, pulverizes etc. Besides this equipment, material-handling equipment like conveyor (belt, chain or screw type), elevators, holding bins and other handling equipment are also frequently used in the processing plants. To meet the above requirement many firms are also involved in the manufacturing of these equipment and machinery. Since, most of the seed processing equipment, dhal milling plants, material handling equipment are manufactured by the unorganized sector and hence equipment and machinery fabricated by them are not of very good quality. However, Bureau of Indian Standards has formulated the codes of manufacturing for these equipment and machinery. Seed processing equipment manufactured in India are generally copy of imported processing equipment. The manufacturers do not use materials of good quality. Framework of the machines is very poor. These machines are not also tested for their efficiency, 

Introduction
The development of the head and neck in embryos involves the formation of two primary sections at the cephalic (head) end) These include:
1. Neural Portion: This part consists of the brain, eyes, and internal ears, along with their supportive structures. In early embryonic stages, the neural region is larger and more prominent. Even as the face develops, the neural portion retains a dominant role.
2. Facial or Visceral Portion: This section encompasses the upper ends of the alimentary (digestive) and respiratory tracts. As the embryo matures, the nose and jaws undergo differentiation and growth, reducing the initial size difference between the neural and facial portions.
Pharyngeal (Branchial) Arches and Their Role
The formation of the face and neck is closely linked with the development of the pharyngeal (branchial) arches. These arches appear as bar-like ridges with intervening grooves on the sides of the embryonic head around the fourth week of gestation. Though similar to the gill arches in fish, these structures in mammals do not develop respiratory functions. Instead, they transform into various permanent structures of the adult face and neck.
• Formation of Pharyngeal Arches and Grooves: Five pairs of pharyngeal arches typically develop, separated by four ectodermal grooves known as pharyngeal grooves. Underneath these grooves, the pharyngeal endoderm pushes the mesenchyme outward, forming pharyngeal pouches.
• Fuj results in a congenital condition known as branchial fistula)
• Role of Pharyngeal Arches in Facial Formation: The first pharyngeal arch on each side splits into an upper maxillary and lower mandibular process, forming the basis of the face. The second and third arches play essential roles in forming neck structures.

Tomato (Solanum lycopersicum L.) is one of the most important vegetable crops cultivated in the world. It is used as raw in salads and also used as cooked vegetable in the preparation of curries. Processed items such as tomato puree, ketchup, pickle, chutney, whole peeled tomatoes and tomato powder are also consumed considerably. It is a rich source of Vitamins A & C. Lycopene and other flavonoids in tomato serve as good source of antioxidants. Tomato occupies 5 million hectares with a productivity of 34t/ha. in the world. In India it is cultivated in an area of 0.88 million hectares with productivity of 21 t/ha.

Principle
Chutney and sauces are important food products prepared both in homes as well as commercially in India. They improve the digestion and are good appetizers. Tomatoes are a good source of vitamin C. Fresh tomatoes are very refreshing and appetizing but can not be stored for a long period. Often they are sold at distress prices during the peak harvest season and nearly 25% of the produce is spoiled due to mishandling and improper processing facilities. Such losses can be reduced by converting fresh tomatoes in to delicious product like paste, puree, juice, ketchup/sauce and powder. Generally, the sugar content in ketchup/sauce varies from 10-26%. The salt content of the product should be 1.3 to 3.4%. Good quality vinegar is essential for the preparation of high quality sauce/ketch up. It should contain 5.0-5.5% acetic acid or glacial acetic acid (100% acetic acid) and should be added when the product has thickened sufficiently. Tomato sauce/ketchup generally contains 1.25-1.50% acetic acid. In order to increase the viscosity and prevent the separation of pulp from clear juice, pectin can be added to extent of 0.1-0.2% pectin by weight of the finished product. Add 0.025% sodium benzoate as preservative to the product before bottling and then pasteurize.

ABSTRACT

Cassava has developed importance mainly as an industrial crop due to its ability to store large quantities of starch (74% to 85% of dry weight) within secondary root structures. Cassava starch is exploited as a source of human food, feed for livestock and as raw material for the food processing and chemical industries. Research accomplishments during the last four decades helped India to have the top position among cassava growing countries with an average yield of 24 t ha-1. However several traits like starch quality, high protein , post harvest deterioration etc can be altered only through the development of transgenics. The present study summarizes the results of the experiments undertaken to optimize the protocol for the development of transgenic cassava through Agrobacterium mediated gene transfer. Friable Embryogenic Callus (FEC) was developed in the cassava var. TMS 60444 us ing Murashige and Skoog (MS) media with picloram (50μM). Experiments were conducted to study the efficiency of different Agrobacterium stains viz, AGL 8, GV3101, C58C1, LBA 4404 and EH105 in transforming FEC. The strains differed in their growth rate. The strains EH105 and AGL were slower in growth as compared to other strains and the protocol was modified accordingly. Among all the strains, EH105 resulted in maximum recovery of transgenic tissues. The paper also summarizes the present status of development of transgenic cassava along with future thrusts.

1. INTRODUCTION

Wild pomegranate (Punica granatum L.) is one of those fruits which has got a great economic importance. The name “pomegranate”is derived from Latin pomum (apple) and granatus (seeded) which has influenced the common name for pomegranate in many languages (like in German Granatapfel, seeded apple). It has widely been cultivated throughout India and drier parts of South-East Asia, Malaysia, the East Indies and tropical Africa. The most important growing countries of pomegranate are are Egypt, China, Afghanistan, Pakistan, Bangladesh, Iran, Iraq, India, Mayamar and Saudi Arabia. In India, it grows in the vast tract of the hill slopes of Jammu and Kashmir and Himachal Pradesh. One of the important centers of wild pomegranate, in Himachal Pradesh is Darlaghat, located about 50 Km from Shimla. The fruit provides substantial income to the farmers particularly where it is found growing wild such as in Mandi, Kullu, Sirmour, Solan, Shimla, and Chamba districts of Himachal Pradesh. It is found very common and gregarious in drier and sub-marginal land of mid hill regions of outer Himalayas at an elevation of 900-1800 m above mean sea level (a.m.s.l). Wild pomegranate is considered as one of the commercial fruits of Himachal Pradesh in the form of dried arils popularly known as “Anardana”. Besides Anardana, other value added products can also be prepared from this fruit as detailed in this chapter.

Dietary constitution of Indian people is mostly cereal based. However, the cereals supply chiefly carbohydrates. Therefore, there is deficiency of protein, minerals and vitamins, especially vitamin A, riboflavin, vitamin C, etc. in the diet. The dieticians advocate the intake of 125 g leafy vegetables, 75 g other vegetables and 100 g root and tuber vegetables (total 300 g) everyday because vegetables provide all the nutrient components like carbohydrates, protein, fat, minerals, vitamins and water along with roughages, which are the essential constituents of a balanced diet.

India has attained food security by increased cereal production due to green revolution. In case of vegetables, although there has been increase in production from 15 million tonnes during 1950 to 94 million tonnes in 2005 but still more vegetables are needed to meet the minimum requirement of at least 250 g per day per capita. There has also been increasing demand for fresh export and for processing industries. Moreover, there is a pressure for increasing vegetable supply caused by constantly increasing population in a world of limited acres.

Addition of new agricultural land is of course not possible. In this situation, plant breeding can be expected to contribute substantially to greater vegetable productivity. This can be accomplished not only by the breeding of basically higher yielding varieties but also by the development of varieties that help to stabilize production through resistance to various biotic and abiotic stresses.

1.    Normally economic growth refers to ___________.

(a) Growth in actual nominal per capita output

(b) Growth in potential real per capita output

(c) Growth in actual real per capita output

(d) Growth in potential real per capita output

2.    Which one of the following is not true about economic development?

(a) Problem of under developed country

(b) Its discontinuous and spontaneous change

(c) It means more output only

(d) Its innovative process

Invertebrate Development

The early stages of embryonic development determine the pattern of structures in the adult organism

The study of Drosophila mutants with disrupted development patterns has identified genes that control development

The generation of a body pattern depends on the positional information that each cell receives during development

The gene products of maternal effect genes are deposited asymmetrically into the oocyte and establish the anteroposterior and dorsoventral axes at a very early stage of development

Gap, pair-rule, and segment-polarity genes act sequentially to divide the Drosophila embryo into segments

The expression of homeotic genes controls the phenotypic characteristics of segments

The developmental fate of each cell in the nematode Caenorhabditis elegans is known

Heterochronic mutations disrupt the timing of developmental changes in C. elegans

6.1. Etawah Pilot Project (1948)

Started in 1948 by Mr. Albert Mayer of USA at Etawah district in UP

Funded by the Government of UP.

6.1.1. Objectives

To know the degree of productive and social improvements, through self confidence and cooperatives

To find how quickly these results could be attained

To know whether the results remain permanent even after the special pressure is withdrawn

To assess how far the results were reproductive in other places.

7.1. Sriniketan Project (1914)

Shri Rabindra Nath Tagore in 1914 established a rural reconstruction institute at Sriniketan involving youth from a group of 8 villages.

Maintained a demonstration farm, a dairy and poultry unit, an outdoor clinic, a department of cottage industries and a village school.

Villages were expected to approach these agencies through village workers who lived in villages to obtain solution of their more pressing problems.

Scheme of land development and tree plantation were given due importance.

Experiment on paddy, sugarcane and cotton were undertaken.

1. INTRODUCTION

Food additives have been used for centuries in food processing practices such as smoking and salting of meat. Prior to the advent of refrigeration, food grown in the summer had to be preserved for the winter. Salt, sugar, and vinegar were commonly used preservatives. Additives serve many roles and their common uses includes maintaining product consistency and palatability, providing leavening or controlling pH, enhancing flavour and imparting colour. A food additive can be defined in many ways. The Codex Alimentarius Commission, which develops international regulatory guidelines for food additives, provides the following definition of a food additive:

"Any substance not normally consumed as a food by itself, and not normally used as a typical ingredient of the food, whether or not it has nutritive value, the intentional addition of which to food for a technological (including organoleptic) purpose in the manufacture, processing, preparation, treatment, packing, packaging, transport or holding of such food results, or may reasonably be expected to result, (directly or indirectly), in it or its by-products becoming a component of or otherwise affecting the characteristics of such food. The term does not include contaminants or substances added to food for maintaining or improving nutritional qualities".

F.A.O and W.H.O in 1956 defines food additives as non-nutritive substances added intentionally to food, generally in small quantities, to improve its appearance, flavour, or storage properties. The definition did not include vitamins and minerals. Food Safety and Standard Authority of India (FASSI) in 2011 defines food additives “as any substances which is added to food for a technological (including organoleptic) purpose in the manufacture, processing, preparation or treatment of such food and affects the characteristics of such food, but does” not include contaminats or any substance(s) added to the food maintaining or improving the nutritional values. Food additives are those added intentionally to foods and are not naturally a part of a food but can be a part of food unintentionally.

Introduction

Oils and fats are utilized both for their food applications as well as for industrial purpose. As a food product, oils and fats are consumed in a variety of forms such as shortening, ghee, butter, margarine, cooking oil, salad oil, etc. Other uses include their usage in the form of lubricants, greases, soaps, cosmetics, paints as well as animal feed and biofuels. Among the Asian countries, Indonesia leads as the prime producer with more than fifty percent of the total palm oil production in the world. As a second-best producer of oils and fats, China was the major manufacturer of rapeseed as well as soybean oil in year of 2018. In general, Asian countries contributed to more than fifty percent of the total fat and oil manufacturing in the world in 2018 (Kushairi et al., 2018). Even though fats and oils are primarily utilized for food applications, there has been an increased interest in their industrial utility for the manufacture of biofuels. Fats manufactured from various animal sources like tallow or lard also contribute to a considerable quantity and compete with vegetable oils for the production of biofuels.

The resources for producing oils and fats are diverse depending on their end use, yet they are obtained from two major sources: animal sources and plant sources. Oils and fats are used as starting materials for the preparation of shortenings, margarines, and other tailored products that further act as functional ingredients in food products prepared by food processors, restaurants, and in home. They have been used for food and other variety of applications since the prehistoric times, due to their easy availability. For example, fatty tissues from animals liberate free-floating fats on being boiled, whereas oil can be pressed out from oil fruits and oilseeds.

The role of dietary oils and fats in human nutrition is also one of the important areas of interest. Over the time, oil and fat industry has provided significant contributions toward nutritional improvement of various products. With the growing concern linked between cardiovascular diseases and consumption of oil and fat products, low-calorie variants of different foods, free or low in cholesterol are also available now. Excessive fat consumption among the populations of developed countries and consequences of obesity has led to the development of reduced-fat and very-low-fat variants of foods.

Morphological characteristics

Abroma is a shrub or small tree that grows to a height of around 3-5 metres. The plant has horizontal and velvety branches that are 10-30 cm long, 6-18 cm wide, oblong or lanceolate, more or less cordate, delicately acuminate, membranous, whole, and three to five lobed with a petiole that is 1-12 cm long. The dorsal surface of the leaves is glabrous, while the ventral surface is hairy.

Floral characteristics

The plant has beautiful and intricate scarlet like flowers (5 cm in diameter) bearing purple colour. The inflorescence is a cyme. The sepals are persistent and lanceolate, measuring about 2.5 cm long. The petals are imbricate and will shortly fall off. Stamens are situated on a short staminal tube, and there are five staminodes. Each cell in the ovary is five lobed and pyramidal, with numerous ovules. The apex, the capsules are obpyramidal, membranous, finely pubescent, and truncate at the apex. Behind each carpel is a triangular wing. December and January are the fowering and fruiting months.

ABSTRACT

Diabetes is a chronic endocrinologic disorder characterized by high blood levels of glucose due to insufficient secretion of insulin by the pancreas or improper utilization of insulin by target cells. More than 120 million people from all over the globe are estimated to suffer from this malady. Many plants and their constituents have become chief ingredients of a number of pharmaceutical preparations used against diabetes. An intensive survey undertaken for over 4 years in the tribal belts of Orissa to identify the plants used in folk medicine and the methodologies employed by the herbal healers have revealed that 35 species are being employed to cure diabetes. Out of 313 patients (185 males and 128 females) interviewed, 211 (67.5%) use Gymnema sylvestre, Momordica charantia, Trigonella foenum-graecum, Tinospora cordifolia, Pterocarpus marsupium, Andrographis paniculata and Rauwolfia serpentina in one form or the other regularly. Multi-plant preparations gave 80% results whereas single plant administration gave less than 50% results. Polyherbal treatment is advocated as multi-pronged attack to achieve desired results.

Diabetes mellitus is a chronic metabolic disorder characterized by persistent hyperglycemia due to impaired insulin production, insulin resistance or both. It affects various animal species, including dogs, cats, horses and occasionally other animals like birds and ferrets. This condition requires lifelong management and, if left untreated, can lead to severe complications such as ketoacidosis, neuropathy and organ damage. Understanding diabetes mellitus in animals, its clinical manifestations and appropriate management strategies is essential for improving the quality of life for affected animals.
Types of diabetes mellitus in Animals
There are two primary types of diabetes in animals, analogous to human
diabetes:
1. Type 1 diabetes (Insulin-dependent):
Most common in dogs,
Characterized by the destruction of pancreatic beta cells, leading to insufficient insulin production,
Typically immune-mediated or idiopathic,

Animal models in diabetes research are very common when most of the existing models are developed as a conventional model either for Type 1 or for T2D. But very often a conventional model of diabetes cannot demonstrate the specific pathogenesis of diabetes related complications. Therefore, the necessity of the individual and specific model for diabetic complications has been raised in the recent years to achieve the authentic outcomes of specific research aims. A number of animal models of diabetic neuropathy have been developed in last few decades approaching from diverse point of views. However, most of them did not receive much popularity because of their considerable number of limitations and disadvantages. In a comprehensive review, Harati [4] reported that the major handicap in studying diabetic neuropathies is the lack of a suitable animal model that addresses acute and chronic events leading to diabetic neuropathy. Hence, in this review, the pathogenesis, advantages, disadvantages, and limitations of several genetic and nongenetic animal models of diabetic neuropathy have been discussed to substantiate their efficacy for human study and in order to guide diabetes research groups to more accurately select the most appropriate models to address their specific research questions.

Collection of faecal samples: To diagnose gastro-intestinal parasites of ruminants or other animals, the parasites or their eggs/larvae must be recovered from the digestive tract of animal or human or from the faecal/stool material.
 
The faecal samples for parasitological examination should be collected from the rectum of animals. If rectum samples cannot be obtained, fresh faecal samples may be collected from the pastures. Several samples should be collected and immediately dispatched to the diagnostic laboratory in suitable containers such as:

Veterinarian’s primary responsibility towards patients and their owners is to establish a correct diagnosis as it is a pre-requisite for rational, effective, eco-friendly and cheap treatment. Diagnosis of a disease is the crux of health problems. An accurate and prompt diagnosis helps in deciding effective treatment options, whereas incorrect and delayed diagnosis often leads to failure in disease outcome.

Diagnosis

The word “diagnosis” is derived from the Greek word “gnosis” that means knowledge. The diagnosis is not only the identification of the disease but also of disease process correlating with clinical manifestations with an understanding of disease etiology and its influence on the structure and function of the body. In fact making diagnosis is itself a process of ascertaining the nature of the disease or disorder from its signs and symptoms differentiating it from similar looking conditions.

1 Diagnosis of a disease includes

a) Determination of specific cause

b) Abnormality of structure and functions produced by causative agent

c) Clinical manifestation associated with the abnormality

d) All of the above

The Central Nervous System not only consists of the Brain but also involves the Spinal cord.

The spinal cord is responsible for conveying information from the Brain to the rest of the body via the peripheral nervous system. The spinal cord can be divided into 4 functional segments.

1. C1-C5 (Upper Cervical Segments)

2. C6-T2 (Brachial Plexus)

3. T3-L3 (Thoraco - Lumbar)

4. L4-S3 (Lumbo-Sacral Plexus )

It is important to note that the motor and sensory function of the pelvic limbs are not only controlled by the Lumbo-Sacral plexus but also involve the Thoraco Lumbar segments (T3-L3) of the spinal cord. It is important to understand not only the neuroanatomy, but also the functional anatomy before approaching a neurological patient.

Introduction

India is the second largest producer of fruits crops in the world but productivity of arid fruit crops especially in western Rajasthan is miserably low.The productivity as well as the quality of fruit crops is affected to a greater extent due to various physiological disorders. The extremes of environmental variables like changing environmental conditions such as temperature, moisture, unbalanced soil nutrients, inadequate or excess of certain soil minerals, extremes of soil pH, poor drainage and deficiency of micronutrients as well as macronutrient causes many more disorders (Kumar and Kumar, 2015). At present, the climatic scenario of arid region is also changing and the problems like frost, temperature fluctuation, moisture fluctuation, high evaporation and low precipitation as well as nutrient imbalances are becoming a challenge for cultivation of many arid fruit crops. Reasons of low productivity in hot arid ecosystem of western Rajasthan are primarily physiological or stress related disorders such as styler-end browning in ber, fruit cracking in bael and pomegranate, internal necrosis in aonla and black and white nose in date palm. The world wide approximately 70% of crop yield reduction is the direct effect by abiotic factors (Acquaah 2007). In this chapter, we discussed the problems associated with ber, pomegranate, aonla, date palm & bael especially physiological disorders which will certainly help the farmers in timely overcoming of these maladies for getting satisfactory remunerative prices from arid fruit crops under hot ecosystem of western Rajasthan.

Abstract

Bacterial diseases of solanaceous crops like tomato, brinjal, chilli, capsicum and potato are the most serious and destructive diseases affecting both under field and greenhouse conditions. Under moist field conditions they can cause epidemics to affect total crop loss, quality of the produce and reduce market value. These diseases can be distinguished from each other on the basis of their symptoms appearance of the various parts of the plant as well as by using advanced techniques of diagnosis. Diagnosis of the disease is important step to detect pathogen at early stage of disease and subsequently manage it effectively in efficient manner.

Keywords: Bacterial diseases, solanaceous crops, symptoms, diagnosis.

Introduction

Bacterial diseases of solanaceous crops like tomato, brinjal, chilli, capsicum and potato are the most serious and destructive diseases affecting both under field and greenhouse conditions. Under moist field conditions they can cause epidemics to affect total crop loss, quality of the produce and reduce market value. These diseases can be distinguished from each other on the basis of their symptoms appearanceofthe various parts of the plant as well as by using advanced techniques of diagnosis. Diagnosis of the disease is important step to detect pathogen at early stage of disease and subsequently manage it effectively in efficient manner. Therefore, the early detection of the bacterium from asymptomatic plants, soil, and water and plant residues could facilitate elimination and certainly reduce the risk of crop loss. Powerful tools for the identification and detection of the bacterium in diverse substrates (plant, seed, water and soil) are available for diagnosis of the bacterial diseases. DNA amplification of pathogen offers many advantages over classical techniques; neither purification nor cultivation of the pathogen is required and the specificity, sensitivity and response time of tests are improved. Nevertheless, the PCR method has not yet become a routine diagnostic tool for many laboratories, mainly because of the inhibition of the amplification reaction by compounds contained in crude bacterial extracts, which give false negative results or low detection sensitivity.

Cancer has gained considerable relevance in companion animals nowadays owing to the increased awareness among the people towards animal sufferings and pain. A proper diagnosis augments a better treatment regime for the suffering animal. Of all species, dog develops cancer twice as frequently as humans. Mammary tumours rank second (behind skin tumors) as the most common neoplasm in dogs but constituting more than 50% tumours in bitches. All suspected growths therefore requires careful appraisal lest it be cancerous. Diagnosis of cancer represents one of the major challenges faced by the small animal veterinarian. In majority of instances, the differentiation of a benign from the malignant tumour can be made morphologically with considerable certainty and for the same, histopathological examination remains the gold standard. A proper histopathological diagnosis however depends a lot on the collection, fixation and processing of tissues. So clinician as well as histopathologist has their own role to play in the final diagnosis of cancer in animals.

A thorough history with complete physical examination including careful palpation of the mammary glands should be performed. As there are chances that dogs may be suffering from any other systemic ailments during middle to old age. In dogs with multiple tumors, all tumors should be excised and their location and size should be noted. Histopathological examination of the excised tumors should be carried out to classify mammary gland tumors. Mammary tumors are classified based on some of the parameters i.e., tumor size, lymph nodes, metastasis. Therefore, to stage a dog with mammary tumors information of these parameters needs to be collected and recorded. If lymph nodes are palpable and/or clinically enlarged, fine-needle aspiration should be performed for cytological evaluation. The technique is less invasive but with higher accuracy. Thoracic radiographs remain the mainstay for diagnosing the metastatic condition. The mammary tumors most commonly metastasize into lungs in dogs. The staging of CMTs becomes necessary as it is likely to confer some prognostic significance and provide guidance to veterinarians to make treatment decisions.

The speed with which changes in the eye can take place is challenging. Ophthalmic problems do not lend themselves to casual examination or ‘trial and error’ therapy. Therefore, to perform safe cataract surgery with good quality outcome, uniform minimum standard of diagnostic and clinical practice needed to be in place.

Although cataract can be diagnosed by routine gross examination ofthe affected dog even then it is mandatary to develop a routine ophthalmic examinations in a consistent and organized manner to make a confirmatory diagnosis and plan the surgical technique for a favourable prognosis. A sequential examination starting right from history up to electroretinography may be performed as under:

History » Gross Examination » Menace response test » Obstacle course test » dazzle or photic blink reflex » Light induced pupillary reflexes (direct/ consensual) » Corneal reflex test »Schirmer’s tear test

Objective: To identify drought tolerant genotypes by PEG method in laboratory.

Background information:

Among different abiotic stresses, drought is considered to be most detrimental for agricultural production. A drought is a period of below-average precipitation in a given climatic region that results in prolonged shortages in water availability, whether atmospheric, surface water or ground water. A drought can last for several months or years. Drought has substantial impact on ecosystem and agricultural productivity.

Some plant species are better able to tolerate drought stress than others. Genetic variability for drought tolerance also exists within many plant species (Blum, et al. 1980). The efficient exploitation of that variability for crop improvement would be facilitated by a fast and accurate screening method for identifying drought tolerant genotypes (Clark and Mc Craig, 1982). Several methods utilizing seedlings to screen for drought tolerance have been developed using an osmoticum such as polyethylene glycol (PEG) (Singh, et al. 1984). The other synonyms of PEG are Poly ethylene oxide (PEO) and Poly oxyethylene (POE); these other chemicals depend on the molecular weight. Chemical structure of PEG may be written as H”(O”CH₂”CH₂)_n”OH (Kahovec, et al. 2002). PEG is used in certain abiotic stress experiments to produce high degree of osmotic pressure, normally in the order of 'tens of atmosphere' PEG produces osmotic pressure due to its flexible nature and water soluble polymer. PEG also makes certain specific interactions with bio-molecules. Due to above properties PEG is used as a specific chemical to produce osmotic pressure in several biochemical and bio-membrane related experiments. For some genotypes, the seedling response apparently is a good indicator of the plant's reaction to drought stress later in its life cycle. However, early screening of seed plants via seedlings tests must still be considered as a method for identifying genotypes that will tolerate drought stress in the vegetative stage (Andersen, et al. 1987).

1. California mastitis test

The California mastitis test (CMT) is conducted during milking of lactating cows. The CMT paddle is used to collect about three to four ml of fore milk from each quarter of cows after discarding few streams to which an equal volume of CMT reagent (composition mentioned below) is mixed immediately by circular motion (Fig. 1). The reaction is graded by intensity of gel formation and color changes as follows:

   Q.1.    How milk sample is collected for diagnosis of mastitis?
    Ans.    At first the end of teat is cleaned with a cotton swab dipped with 70% alcohol, then the dirt and wax are removed from the external sphincter by pressure. After this milk sample is collected in screw cap bottle.

    Q.2.    When milk sampling should be done in suspected cases of tubercular mastitis?
    Ans.    Last few streams of milking is crucial in cases of tubercular mastitis.

    Q.3.    What is the effect of freezing of milk sample on bacterial count?
    Ans.    On freezing of milk, Arcanobacter pyogenis and E. coli counts are decreased, coagulase negative bacteria counts increases whereas, Streptococcus and Staphylococcus spp. counts may be unaffected or increased.

    Q.4.    Which media is suitable for initial isolation of bacteria from milk samples?
    Ans.    Blood agar. However for isolation Streptococcus agalactiae selective media is required

DSM-5 and Diagnosis of Mental Health Issues
Mental health is a fundamental aspect of overall wellbeing and the accurate diagnosis of mental health issues is crucial for providing appropriate and effective treatment. The Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition (DSM-5) is the latest reference guide used by mental health professionals to diagnose mental health conditions (Kapadia et al., 2020). The current edition, DSM- 5, builds on the goal of its predecessors of providing guidelines for diagnoses that can inform treatment and management decisions. The DSM-5 provides a comprehensive and standardized framework for the classification and diagnosis of mental disorders.
The DSM-5 represents a significant advancement in the field of psychiatry, as it incorporates a cross-cultural assessment of mental health issues and an updated classification system. (Petito et al., 2020) This detailed diagnostic criteria helps clinicians accurately identify and differentiate mental health conditions, enabling them to develop tailored treatment plans.

Diagnosis of Poisoning    
Three types of diagnosis
 a) Tentative
 b) Presumptive
 c) Confirmative based on the following conditions
 1. History
 2. Physical examination and clinical evidences    
Hyperthermia- Organochlorine;    
Hypothermia- OP compunds    
Miosis: Opium 
Mydriasis- Belladona    
Dryness of mouth (Xerostomia)- belladona;    
Moist and Hypersalivation- OPC, Carbamates and synthetic pyrethroids 
Bitter Almond    smell-Cyanide
Garlic odor in expired air- Phosphorus, Arsenic    
Mice smell- Hemlock    
Rotten Garlic/Horse raddish smell- Selenium Poisoning

• General considerations

 • Tuberculosis

 • Johne’s disease

 • Brucellosis

 • Infectious bovine rhinotracheitis

 • Campylobacteriosis

 • Tichomonosis

Introduction

Diagnostic biochemistry, is a branch of laboratory medicine that focuses on the analysis of bodily fluids to diagnose and monitor diseases. This field plays a crucial role in understanding the biochemical mechanisms underlying various conditions, enabling accurate diagnosis, prognosis, and management. The biological samples for diagnosis include blood, urine, cerebrospinal fluid, pleural fluid and tissues.

Factors Affecting Serum Enzyme Activity or Protein Level

1. Organ Mass and Enzyme Tissue Concentration:

• Higher enzyme concentrations in organs can lead to greater increases in serum enzyme activity during disease. For instance, the liver’s large mass contributes to potential increases in serum ALT activity.

2. Cell Location

• The proximity of cellular enzymes to the blood, urine, or other fluids determines the fluid in which an enzyme’s activity will increase upon cell injury. For example, renal tubular gamma-glutamyltransferase (GGT) is released into urine, not blood, following injury.

Diagnostic biomarkers are measurable biological molecules that provide information about physiological or pathological conditions. This chapter examines the use of metabolomics for discovering, evaluating, and validating biomarkers for disease diagnosis, prognosis, therapeutic monitoring, and personalized medicine. It discusses different types of biomarkers, metabolomic biomarkers, biological samples, analytical platforms, biomarker-discovery workflows, and statistical analysis. Applications in cancer, diabetes, cardiovascular, liver, kidney, neurological, infectious, and metabolic diseases are considered. The chapter also explains the role of metabolomics in drug discovery, toxicology, nutrition, and clinical research. Challenges involving biological variability, analytical reproducibility, data complexity, and clinical validation are addressed alongside future opportunities involving AI and multi-omics.

Diagnostic techniques are routinely used for diagnosing different surgical conditions. The most frequently used imaging techniques in veterinary practice are radiography and ultrasonography. Radiography and ultrasonography are not suitable for each organ in large animals. In small animals most organs are suited to ultrasound images except bones and gascontaining structures such as lungs etc. Bone and gas reflect ultrasound and do not allow the sound waves to form image therefore radiography gives better diagnostic images for these organs. The liver, spleen, kidneys, prostate, gravid uterus and fluid containing organs like urinary bladder, gall bladder and major blood vessels can be better imaged with ultrasound. In buffaloes in surgical section important clinical conditions relate to urinary and digestive systems, mammary gland and perineal area.

1. Urinary System: In bovine, the cases of obstructive urolithiasis are commonly observed. These types of cases are more common in bullocks and in buffalo calves. In these cases radiography is not so useful. Sonography can be tried to take the image of ruptured urethra in bullock and can be compared with radiography. Due to ruptured urethra there will be accumulation of urine in the s/c tissue of prepuce. Due to fluid density the image will be radioopaque and will appear white and no detail of the organ can be seen. The ultrasonogram (USG) will show images of glans penis and its coverings. Different diameters of urethra and a point of rupture just behind to glans penis can be make out. Due to obstruction, The B.calves either will pass drop wise urine or there will be complete stoppage of the urine. These cases can also be easily diagnosed by ultrasonography.

The aquaculture sector in India is expanding rapidly, playing a critical role in enhancing rural livelihoods, ensuring food security, and boosting export revenue. However, disease outbreaks remain a persistent hurdle, often leading to substantial financial losses for fish farmers. To mitigate this, the deployment of user-friendly, efficient, and rapid diagnostic tools at the field level has become increasingly vital—especially in areas with limited access to laboratory facilities.
Fish disease diagnostic kits, based on immunological and molecular technologies, are designed to detect specific pathogens responsible for viral, bacterial, parasitic, and fungal infections. A major benefit of these tools is their ability to deliver results swiftly—typically within minutes or hours—enabling prompt action. In India, research bodies such as ICAR-CIFA, ICAR-CIBA, and ICAR-NBFGR have played a key role in developing and adapting these tools for local aquaculture conditions.
Efficient nucleic acid extraction is a cornerstone of field diagnostics. Rapid DNA/RNA extraction kits that function without centrifuges and under varied environmental conditions have made sample processing quicker and easier. Mobile diagnostic vans—equipped with portable lab equipment—have also been introduced in states like Andhra Pradesh and Tamil Nadu for on-site disease surveillance and farmer training.
For detecting parasites, wet mount kits containing preloaded reagents like iodine and saline are used for direct microscopic examination of fish tissues. These kits help identify parasites such as Trichodina, Ichthyophthirius, and monogeneans, aiding in immediate diagnosis and treatment advice.

12.1. Introduction

The protocols provides guidance on the structure and content of the International Plant Protection Convention (IPPC) diagnostic protocols for regulated pests. The protocols describe procedures and methods for the official diagnosis of regulated pests that are relevant for international trade. They provide at least the minimum requirements for reliable diagnosis of regulated pests.

This standard sets the framework for the content of diagnostic protocols, their purpose and use, their publication and their development. Diagnostic protocols for specific regulated pests are included as annexes to this standard. Information relevant for diagnosis is provided in the diagnostic protocol on the specified regulated pest, its taxonomic position, and the methods to detect and identify it. Diagnostic protocols contain the minimum requirements for reliable diagnosis of the specified regulated pests and provide flexibility to ensure that methods are appropriate for use in the full range of circumstances.

The methods included in diagnostic protocols are selected on the basis of their sensitivity, specificity and reproducibility, and information related to these factors is provided for each of these methods. Detailed information and guidance for the detection of pests is provided on, for example, signs and/or symptoms associated with the pest, illustrations (where appropriate), developmental stages of the pest, and methods for detecting the pest in a commodity, as well as methods for extracting, recovering and collecting the pests from plants.

Introduction

To maintain optimum reproductive status early diagnosis of reproductive problems is important. Various advance diagnostic techniques are available for diagnosis of reproductive problems. Some of them are still in early stages of development or some though available; are not widely used due to various constrains.The various diagnostic techniques used in animal reproduction are ultrasonography, Doppler ultrasonography, endoscopy, laparoscopy, hormonal assays, vaginal cytology, x ray etc.

Accurate history and Thorough examination of reproductive tract

Accurate history is a powerful tool of enormous clinical value. Behavioral problems due to pain or psychological factors can also be ruled out on the basis of adequate history of the patient. Tentative differential diagnosis can be made by taking history of previous breedings.Through digital and vaginiscopic exanimation of reproductive tract helps to rule out anatomical abnormalities, which may interfere with natural mating. It also helps in diagnosis of fertile phase of oestrus cycle. In cases of refusal to mate due to anatomical problems like septum, narrow passage or due to behavioral problems AI can be performed. Vaginoscopic examination aids vaginal cytology in deciding right time of natural service or insemination in proper breeding management or abnormal oestrus cycle.