eChapters

Various products of karanj (Pongamia glabra) are utilized for industrial, health and animal agricultural application in the Indian subcontinent. Despite a rich source of protein (CP 28 —34 %), karanj (honge) cake is found to be slightly bitter in taste and toxic owing to the presence of furanoflavonoid (Karanjin), restricting its safe inclusion in the livestock diets. Though a non polar solvent, soxhlet extraction of karanj seed cake with petroleum ether/ acetone drastically reduces karanjin content. The residual cake left after solvent extraction is proved to be safe as animal feed supplement.

Estimation of karanjin

The samples can be analysed for the toxin, karanjin as per the method of Prabhu et al. (2002).

Extraction of Karanjin from Raw and Processed Cake

Exactly 25 gm of ground and thoroughly mixed sample is weighed and transferred into the thimble and extracted for 12h using 200ml of freshly distilled methanol as solvent. Methanol extract is then cooled and filtered into preweighted round bottom flask using Whatman No.1 filter paper.The excess methanol is distilled off under vacuum. The flask containing the extract is weighed again to obtain the weight of extract by subtracting the empty flask’s weight. Sufficient amount of moisture free extract is transferred into vials for further analysis.

High performance liquid chromatography (HPLC) was developed during late 1960s and early 1970s. Today it is a widely accepted separation technique for both sample analysis and purification in a variety of areas. HPLC, basically a highly improved form of column chromatography and popularly known as liquid chromatography, like other forms of chromatography, whether paper, thin layer or gas chromatography, needs two essential components, the stationary and the mobile phase. It has the unique ability to separate and quantitate residues of polar, non volatile and heat labile chemical compounds. HPLC uses high pressure pumps up to 400 atmospheres that makes it much faster, short and narrow columns packed with micro particulate phase and a detector. To affect a separation, mixture to be analysed is dissolved in a solvent (ideally this solvent is identical to mobile phase) and then forced to flow through analytical column along with mobile phase. In the column the constituents of mixture get resolved and are detected with a suitable detector by a proper choice of column, mobile phase and detector. HPLC is a technique having high degree of versatility. There are several excellent texts published over the last decade which may be consulted for general information on the instrumentation in liquid chromatography

In the present era, residue analyst is exposed to array of analytical choice and as per International regualtions, presence of pesticide residues in environmental components and food commodities are becoming more stringent.Generally, multiresidue methods are based on GC equipped with selective detection either electron capture detector, nitrogen phosphorous detector, flame ionization detector etc. One most widely applied technique in pesticide residue analysis is HPLC, equipped with different detectors. Another up-datemethod for confirmation is gas chromatography-mass spectrometry(GCMS)and HPLC-MS applyingparticlebeam or thermo sprayionisationtechnique. Recently one more analytical technique which has gained ground in the field of pesticide residue analysis, is high performance thin layer chromatography (HPTLC).The criteria of good analytical technique like simple, rapid, flexible, versatile, inexpensive, easily documented and validated has been the inherent advantage of HPTLC. Strictly speaking, HPTLC is not different in principleor application from conventional TLC, but due to improvements in materials, its effectiveness has also enhanced.The technique has a potential to operate upto femtogramlevel. HPTLC is a sophisticated and automated form of TLC. It is a technique which can generate about 5000 or more theoretical plates. The high performance in TLC is achieved by optimized plate coating materials, by improved sample application techniques, and by developing newmethods for the development of mobile phase. Thus HPTLC is an instrumental approach at different stages of TLC. A flow chart for HPLC principle is shown in Figure 8.1. The principle of separation is based on adsorption and this leads to easyseparationof isomers, includingchiralcompounds.Main difference between HPTLC and TLC is given in Table 8.1

High pressure (or performance) liquid chromatography (HPLC) is more versatile than GC since it is not limited to volatile compounds. The choice of mobile and stationary phases is wider. HPLC has high resolving power, separations are speedier, quantifications are more precise and it is in general, more reproducible. Liquid chromatography basically involves separation of components due to differences in the equilibrium distribution of sample components between the mobile (liquid) and stationary phases.

1. Introduction

Fruit products have their unique identities having large content of micronutrients, polyphenols, vitamins, flavonoids and other nutritional compounds. Characteristic appearance and flavors distinguish among various fruit based products like juice, puree, jam, jelly, squash and other several varieties. Main concern of this fruit processing industry is the stability of the products throughout the post processing storage. Thermal treatment as mean of preservation of these products is the common process implemented so far. It ensures the safety of the product but lacks in retaining the nutritional intact that like of fresh fruit. Today’s consumer demand for minimally processed fruit products and “natural” fruit products tend to think of alternative thermal processing.

High pressure processing (HPP), a relatively new concept compared to conventional thermal processing, also sometimes known as high hydrostatic pressure (HHP), or ultra high pressure (UHP) processing, is a non-thermal food processing method that subjects liquid or solid foods, with or without packaging, to pressures ranging between 50 and 1000 MPa. HPP has already been established itself as an alternative to thermal processing. In food processing it is of great interest due to its ability to inactivate food borne microorganisms and enzymes, at low temperature, without the need for chemical preservatives and it is less detrimental than thermal processes to low molecular weight food compounds such as flavoring agents, pigments, vitamins, etc., as covalent bonds are not affected by pressure. Pressure-treated foods have sensory properties similar to fresh products, which is a major advantage in fruit processing as it matches consumer demand for healthy, nutritious and ‘‘natural’’ products. HPP of fruit products offers the chance of producing food of high quality, greater safety and increased shelf life (Hayashi 1989; Gould 1996; Butz and Tauscher 1998).

4.1 Introduction

The purpose of processing the food is to make it safe and healthy for human consumption. The term food processing refers technologically anything, which transforms raw ingredients into consumer ready products, with the aim of stabilizing food products by preventing or reducing deteriorative changes in quality. The objective of food manufacturers is to develop, modify and employ those processing technologies that retain or create desirable sensory qualities in food and eliminate or reduce undesirable changes due to processing.

The purpose of food processing is to increase the shelf life of food before spoiling (preservation); to remove, destroy or inhibit pathogens and toxins (food safety); to change flavor, texture, aroma, color or form (variety); to reduce preparation times and make food more portable (convenience); to restore and/or raise nutrient levels in food (nutrition).The various food processing methods used extensively in food industry include physical (high temperature, freezing, chilling, dehydration, packaging), chemical (control of storage gases, reduction of water activity, pH, addition of chemical additives or spices) preservation methods.

Traditionally, food preservation has been associated with thermal treatments alone or in combination with biochemical or chemical methods. For example, the thermal processing of milk is done various thermal treatments viz. at 63^oC for 30 min for low temperature long time (LTLT), 72^oC for 15 seconds for high temperature short time (HTST) pasteurisations and up to 140^oC for a few seconds for ultra high temperature (UHT) treatment. However, such thermal treatments effectively reduce level of microorganisms but in long-term preservation, it may lead to losses of desired organoleptic attributes and destruction of temperature liable nutrients and vitamins. The limitations involved with thermal processing has been overcome by employing chemical preservation, cool chain management and process hygiene in the formulation and production of food products.

Food and nutrition are necessary for all living beings to maintain good health[1]. Thermal treatment is commonly used for preservation of food. Although application of thermal treatment can ensure food safety, it may cause undesirable changes in the nutritional, sensory and functional properties of food products[2]. Due to growing awareness of connection between the diet and the health, there is an increase in demands for fresh-like, palatable, additive-free and microbiologically safe food products with high nutritional value and quality[3].

Abstract

High Tech interventions begin with acute desire and demand for high productivity and profit from Agricultural activity. High Tech cultivation implies judicious use of space, time and inputs; nothing more and nothing less. It is interesting to note that the different components that were listed in each case above are known to scientists and extension workers and to a lesser extent to farmers also. Piecing them together to formulate a practise is the novel thing. The major components of high tech cultivation assume the highest priority by virtue of the fact that the country has shortage for water and fertilizer and both are essential for any type of crops. Different components of high tech intervention can be used for growing crops, also for the growers to enhance production and quality .High tech intervention includes a number of components like- uniform virus free planting material, drip fertigation technology, assistance to post harvest management, and training and guidance to farmers, value addition opportunity, technical support to the farmers etc. These high tech interventions are introduced to other crops- a host of vegetables, fruits like Pomegranate, Cashew and Guava, and agricultural crops like, Cotton, Pulses, Sugarcane, and even cereals like, maize, wheat and rice. Another important aspect of such interventions is that only if concomitant interventions in marketing of produce are made, the whole exercise would bring profit to the farmers.

ABSTRACT

Commercial propagation using tissue culture techniques started in seventies for orchids in USA. Since then, the science and technology of tissue culture have advanced particularly after the development of the most widely used medium by Murashighe and Skoog (1972). The science has led to the development of the fascinating area of gene transfer. Today, micropropagation protocols are available for almost all horticultural crops. But, there are certain grey areas where research need to be intensified to solve the problems confronting certain difficult to propagate species belonging to plantation and spice sectors. The major area that needs to be addressed is recalcitrance.

Introduction

Raising milk and meat production are long-standing global issues. Researchers are learning that present breeding efforts won't be sufficient to meet anticipated future food demands given the current climate scenario. The animals that influence the overall yield of the field must be screened as soon as possible. In order to build more successful modern breeding programmes, high-throughput phenotyping is currently being included into conventional breeding operations. Despite the public accessibility of their genetic information online, there is still a lack of phenotypic data on the genomes of various animal breeds because environmental factors make it more difficult to define phenotypes and increase the likelihood of measurement error (Rahaman et al., 2015). Genomic selection (GS) is a strategy that predicts the genomic estimated breeding values of lines in a breeding population using information from genome-wide markers (Meuwissen et al., 2001). When compared to other conventional methods like marker-assisted selection (MAS), genomic selection has some inherent advantages. These include boosting genetic gain by reducing breeding cycles and acquiring minor effect loci based on markers dispersed throughout the entire target genome. Reliable phenotypes and genotyping are both necessary for the training of accurate prediction models for genomic selection. Phenotyping is regarded as a significant factor restricting genetic improvements in animal breeding due to high labour and time expenditures. Traditional approaches of phenotyping are prohibitively expensive, time-consuming, and slow.

Abstract

The Himalaya is one of the youngest and the richest mountain ecosystems encompassed with a variety of species of both flora and fauna. Varying in climatic conditions coupled with altitudinal gradients and unique topography leads to the formation of different types of forests, which varies from tropical to the temperate and alpine to no timber line zone and snow-clad meadows. The present study aimed to investigate the potential of medicinal plants growing in valleys and mountains of district Doda to cure various ailments of the local inhabitants including animal health care. A total of 113 species belonging to 104 genera and 55 families of angiosperms, gymnosperms and cryptogams (lycophytes and ferns) were recorded and reviewed from published documents used for treatment of different diseases in the regions. Out of the total 113 species, 17 species were of trees, 14 species were shrubs, 78 types of herbaceous plants and 4 species of climbers. Asteraceae, Lamiaceae, Rosaceae, Ranunculaceae, Polygonaceae, Solanaceae, Apiaceae, Pinaceae, Fabaceae and Berberidaceae were recorded as the dominant families of the study area. The data were collected while interacting with local people as they possess rich traditional knowledge regarding the use of different herbal plants and medicine.

Introduction

Arid and semi arid parts of the India are known as Seed Spices Bowl (Rajasthan and Gujarat) and contribute more than 80% of total seed spices production. Total 109 spices are listed by ISO and 63 spices are grown in India and out of which 20 are being classified as seed spices. Out of 20 seed spices, ICAR-NRCSS working on 10 most important seed spices namely cumin, coriander, fennel, fenugreek, ajwain, anise, caraway, celery, dill and nigella (NRCSS Vision, 2050). Seed spices also grown in some other states on considerable areas such as Uttar Pradesh, Madhya Pradesh, Bihar, West Bengal, Orissa, Punjab, Karnataka and Tamil Nadu. Since there is a large scope of seed spices by introducing them in new areas, the higher yields can also be achieved effortlessly by implementing new technologies. Among seed spices, maximum area is under cumin followed by coriander, fennel fenugreek, ajwain, celery, nigella, dill, anise etc. Being seasonal crops, these are grown extensively in rotation with food crops and also as inter/mixed crops under rainfed/irrigated conditions. All these crops mostly grown in rabi season. India is the largest producer, consumer and exporter of seed spices in the world. Out of total area and production in spices, seed spices contribute about 51.79% of total area and 19.06% of production of spices in the country. The area under seed spices is about 1.74 million hectare and 1.45 million tonnes. India is consistent source of seed spices for importing countries worldwide. There has been ever increasing demand of seed spices and importing countries look at India for quality produce of seed spices.

Introduction

Vegetables are one of the potential crops in meeting the food requirements and improving the nutritional status of our people. They play a vital role in crop diversification, employment generation, and nutritional security and in improving the economic conditions of farmers.

Our country has made a tremendous progress in vegetable production and ranked as second largest producers of vegetables in the world with a total production of more than 125 million tons per annum. Our estimated demand is about 225 million tons by 2020 AD. This target can be achieved through use of improved vegetable varieties, hybrids and production technologies even with diminishing land and water resources.

In Indian vegetable scenario, IARI has contributed significantly by developing more than 168 improved varieties and 24 hybrids in different vegetables. Apart from high yielding, many of these varieties/F₁ hybrids are resistant to biotic and abitic stresses and also suitable for processing, value addition and export. Besides developing large number of improved varieties and F₁ hybrids of vegetables, IARI has developed/standardized improved hi-tech vegetable nursery and off-season production technologies which require commercialization by the entrepreneurs as these can bring quantum improvement

Abstract

Greenhouse vegetable cultivation is gaining popularity for reasons such as high productivity of quality produce round the year with enhanced input use efficiency. As agriculture has a major share in our economy and if India has to emerge as an economic power in the world, the productivity should substantially increase from the present levels. We need a new and effective technology which can constantly improve the productivity, profitability and sustainability of our major farming systems. One such technology is the green house vegetable production technology. At Indian Institute of Horticultural Research, Bangaluru, research on greenhouse vegetable production is in progress since the year 2000, resulting in the development of many technologies which have made possible to realize high yield levels in tomato, capsicum and cucurbits that are comparable to international standards of greenhouse productivity. Tomato and bell pepper yields have touched 300 tonnes and 200tonnes/ha/year, respectively and cucurbits 80 to 100 tonnes/ha/crop of 3 months. Vegetable cultivation in cost effective greenhouse is a technical reality in India, which is being adopted by resourceful farmers in and around big towns and cities as a peri-urban horticultural system. Such production systems have not only extended the growing season of vegetables and made their availability round the year but also opened the possibilities of growing new vegetables such as coloured capsicums, cherry tomatoes, seedless watermelons, icebox melons, sweet cucumbers, coloured cabbage, etc., besides tomato, cucumber, eggplant and climbing beans. Quality seedling production is one more important greenhouse activity in the present agricultural scenario as more and more F₁ hybrids are being adopted by the farmers.

High Yielding Variety Programme (HYVP)

High Yielding Variety Programme (HYVP) was launched in 1966, which helped the country in attaining self-sufficiency in food. The technological development did not remain confined to the introduction of high yielding crop varieties alone. These were combined with the application of high analysis and balanced fertilizer, irrigation, plant protection, improved implements etc. which made a ‘Green Revolution’possible in the country.

India is the second-largest producer of fruits in the world after China. However, the average productivity of fruits in India could be higher compared to many developed countries. The main reasons for low productivity are old and senile orchards, low-yielding varieties, poor orchard management, and inadequate technological upgradation and adoption by the growers. Presently, due to urbanization and population explosion, there is a continuous decline in the availability of cultivable land; rising energy and land costs, together with the increased demand for horticultural produce, have given thrust to the concept of high-density planting (HDP) in fruit crops. However, at the same time, it is now realized that there is limited scope for a quantum jump in fruit production in traditional areas. Therefore, the alternative is to expand under non-traditional regions like arid and semi-arid areas of the country. India’s hot arid and semi-arid regions are spread mainly in Rajasthan, Gujarat, Andhra Pradesh, Telangana, Punjab, and Haryana. The vast land resources offer an excellent opportunity for fruit production in the Indian arid zone, which is spread over about 31.70 m ha, of which 41.50% is arable and 19% is a cultivable wasteland (Table 1).

High-pressure processing (HPP) is a novel method for non-thermal preservation technique of food that inactivates harmful pathogens and vegetative spoilage microorganisms. HPP is an alternate, non-thermal food processing method, wherein the food is subjected to a very high pressure range from 100 -800 MPa (1MPa = 145.03 Psi or 10 Bar). HPP is also referred as High hydrostatic pressure processing, Ultra high-pressure processing, Hyperbaric processing and Pascalization (Rao et al., 2014).

8.1. Biofloc aquaculture

Among the innovations in the field of aquaculture, biofloc technology (BFT) has attracted great attention due to its ability to foster the goals of sustainable aquaculture (Khanjani et al., 2022a). If implemented correctly, BFT capitalizes on the existing organisms in water to significantly reduce water use and environmental impact and increase biosafety at farms (Avnimelech, 2012).

Abstract

Rural indebtedness is an old problem in India. In the 19^th century commercial banking was absent in rural areas and farmer were entirely in the hand of usurious moneylender. Since the British rule, the central government has been trying to extend institutional lending to the rural agriculture sector. To provide credit to the agricultural sector, a multi-agency approach involving co-operative banks, scheduled commercial banks, and RRBS has been used in India. Adequate and timely loan availability at reasonable rates is the basis for agricultural finance policy. The institutional structure for agricultural lending has improved over time in terms of scope and reach. India’s higher education institutions have been focusing their efforts on advancing agricultural finance.

Definition

Fracture of humerus bone and femur bone are generally categorised under higher level fracture.

Methods Used For Fracture Repair

These level fractures are repaired appropriately by intramedullary pinning and Thomas splint application.

Only pinning or only Thomas splint are not the method for its repair as the distal fragments of bone get tourqed by pinning only and the fracture fragments get distracted by simple Thomas splint application.

Hence after pinning a humerus or femur bone, the limbs are kept immobilised perfectly by Thomas splint application.

When pinning as well as Thomas splint application are not possible the fractured bones are kept immobilised with fevicol gum bandage only. So for this purpose modification of velpeau sling is generally adopted and animal is restrained within limited space for 21 days.

Definition

Any fracture or discontinuity in bony structure like scapula and pelvic girdle present above the shoulder joint and hip joint respectively are called as highest level fracture.

Aim

To know the method of repair of highest level fracture.

Procedure

As there is no satisfactory method of external immobilisation till date, generally animal is kept under restricted movement. Sometimes application of charge (glue bandage) externally may give some satisfactory result.

INTRODUCTION

According to analyst firm Gartner, Big Data is at the portion of the hype cycle called the "peak of inflated expectations." The business world is awash with all sorts of claims about the magic of Big Data and how it will transform industries by increasing productivity and profits and opening up opportunities that nobody even knew existed. But this will only happen if companies are able to hire enough people who actually understand what Big Data is, how to collect it, and preserve it. Computing and analytical skills are also required to get Big Data to reveal its hidden secrets and visualise it in novel ways. And there unfortunately, is the rub. There are just not enough data scientists, people with the required skills to satisfy this unmet demand. The shortfall in Big Data experts is set to rise and in the UK alone, one digital industries employer body has predicted there will be a need for 69,000 of these experts in the next five years. This claim is not original. Back in 2011, McKinsey & Co was claiming a US shortfall in Big Data experts of 140,000 - 190,000 by 2018.The shortfall in Big Data experts is being manifested in a number of ways. The first and most obvious is through recruiters casting an ever-widening net in their search for appropriate talent. There is some agreement that Big Data analysis and data visualisation requires skills in computing as well as statistics and mathematics. This has meant that university graduates with statistics, computer science and engineering have been the main source of potential employees. Lately this has widened to include subject areas such as astrophysics and computational chemistry.

INTRODUCTION

Assam is a heartland of indigenous groups. Since hundreds of years, many indigenous groups have been living in the state, maintaining their originalities in every sphere of life with difficulties. The tribal-indigenous groups constitute almost 12.4% of the total population of Assam. (Sentinelassam.com). Fishing is one of the major livelihood option of many indigenous fishers as the state is blessed with huge water resources with about 3.91 lakh hectare of water area in the form of rivers, beel, derelict water bodies and ponds and tanks. Besides swamps and low-lying areas, two major rivers and their tributaries have a high growth potential for fishing and could play a pivotal role in the socio-economic development and employment generation in the state. Indigenous fishers have developed a close and unique connection with the water resources and its environment in which they are depended for generation to generation for their livelihood. They have generated unique system of knowledge, innovation and skills relating to the uses and management of fishing activities. Over centuries, people have learnt how to get food and to survive in different environment. Farmers are very keen to observe the problems arising during fishing and seek solutions to adjust to their local environmental conditions. They continuously adapt and manage their fishing systems and in this way, they develop a rich storehouse of practical knowledge on their situation.

Technologies for cultivation of crops are changing over the years. Adherence to the principles of conservation, high input-use efficiency and higher productivity with optimum inputs and the very idea of crop production as a business venture; all these are leading to increased adoption of high technological (high-tech) crop production practises.

Besides diversities and advantages in hills, the communities sustain largely on subsistence farming due to following constraints A.Natural constraints in hills There are large number of Inherent limitations in hill agriculture like, remote areas, vulnerability, marginality , eroded soil and short growing seasons. These Inherent constraints can be judged by the use of unsustainability indicators of hill agriculture which further showed increased in soil erosion rates from sloping farmlands , leaving of agricultural land due to decreasing soil fertility, appearance of stones and rocks on the cultivated lands, reduction in forest and grazing areas and more emphasis on monocropping that widened the cycle of inadequate food production and lead to food insecurity and increasing unemployment and frustration (Table 3) (Shrestha, 1992). These natural constraints are.

Pulses occupy an important place in hill agriculture. They are complementary to cereals in production and consumption being an important source of protein and nitrogen fixing ability which helps in improving soil health. Thus, cultivation of pulses has a positive relationship with our environment, human health and food security. Pulses produced in the hills are famous for their cooking quality, taste and flavour.
 
Himachal Pradesh has emerged as a model hill state in which agriculture is a dominant economic activity and major source of employment and income to the rural people. Being a hilly state, agriculture is practiced on about 10% of the geographical area. Out of which nearly 80% is rainfed. During the last three decades, the farming scenario has changed significantly. The area under fruits, vegetables and cereals has increased tremendously at the cost of pulses and oilseeds. There is, therefore, an urgent need to reverse this trend as pulse cultivation will not only increase soil health but save precious foreign exchange.

ABSTRACT

Plants play an important role in human survival and contribute to sustainable development as they are the major source of different bioactive compounds such as taxol, digoxin, reserpine and vinblastin used directly as drug and medicines for human diseases. As plants are basic need and requirement for living, they are used as herbal remedy, and known to produce lead compounds for semi synthesis of higher activity or lower toxicity. Indian Himalaya regions are repository of more than 8,000 plants species and out of which 50% are endemic and unique due to thepresence of morphological and chemical structure of peculiar characters. Most of the plant used in Indian traditional system of medicines such as Ayurveda, Unani, Siddha or Amchi described for different formulations and ethnomedicinal applications had distributional range restricted to the hills and the valleys of Himalayas. Hence, in this communication, an attempt has been made to present economic plants of Himalayan origin used as ethnomedicine, wild food plants as vegetables, herbal teas, homemade soups, wild salads, chutney, underground snacks, alcoholic drinks, nutrients as burnt powder and other human usages. Besides, this unique Himalayan plant has potential to be used as wild source for the development of new drugs and medicines which can sustain future to human survival and had the ability to produce medicine against deadly diseases. It is estimated that 80% of the world population uses traditional medicine made from herbal botanicals to cure disease and illness. It is of the record that 122 compounds obtained from ethnomedicinal plants is globally used as drugs. This indicates that tribal knowledge on plants and drug discovery cannot be separated, and newdrugsfor uniquedeadly diseases can be discovered only from ethnobotanical leads. More than 5,000 species reported from Indian Himalayas are of ethnobotanical values and they are used as medicine or wild food by different tribal communities. It can be concluded that Himalayan tribals are endowed with strong culture associated with botanicals and have age-old tradition with respect to the use of wild plants as food and medicine. Plants provide a real substitute for the primary health care systems in hills and valleys, and definitely going to serve as jewel for mankind in near future to come.

Morphological characteristics

Plant is terrestrial glabrous herb attends height of 20-25 cm. Tubers slightly flatten ed, palmately divided into 3-5, finger like lobes. Flower stalk upto 90 cm Long, erect, hollow, leafy throughout or the lower portion bear few sheathing scales. Leaves are 4-6, cauline, leaf blade oblong to linear lanceolate, 8.0-15.0cm x 1.5-3.0 cm in size, base sheathing, apex obtuse or acuminate.

Floral characteristics

Inflorescence is raceme, 5.0-15.0 cm long, crowded with many flowers. Flowers are purple and the bracts green, narrowly lance shaped, lower bract longer than the flowers, upper slightly shorter. Flowers are about 1.8 cm long, including the curved spur. Sepals and petals are nearly equal. Three of them form a hood and the two sides spread outwards. The lip is rounded and shallowly 3-lobed, spotted dark purple. Spur straight, cylindrical, nearly as long as the ovary; column very short; anther adnate to its face, cells diverging; pollinia 2, caudicles attached to 2 small globose, viscid glands enclosed in a minute pouch overhanging the broad, 2-lobed stigma.

Distribution

This attractive terrestrial orchid grows in damp pastures of Himalayas from Kashmir to Nepal at the altitude of 2500-5000 m MSL.

ABSTRACT

Saussurea costus (Falc.) Lipsch. is a perennial rhizomatous herbaceous plant, commonly called as kuth had a wide application in drug discovery programme and indigenously known for its medicinal usage in Indian system of medicine. Wide distribution of this species recorded from Himalayas and Indo-Mynamar hotspot biodiversity regions. This species is traditionally used for treatment of several seasonal diseases happen to occurs in hilly and mountaneous regions. It has been scientifically validated that this species possess various biological activities such as anti-microbial, anti-cancer, anti-arthritic, anti-convulsant, anti-oxidant, angiogenesis effect, hepatoprotective, gastro-protective, anti-obesity, and anti-inflammatory due to the presence of various bioactive principles such as costunolide, germacrene, lappadilactone, isodihydrocostunolide, cynaropicrin, linoleic acid, cyclocostunolide, alantolactone, isoalantolactone, sesquiterpene-saussureamines and several others in minor quantities. This species naturally grows in wild and mostly collected from the wild for its herbal usages, and this wild collection activities put S. costus under threatened and critically endangered categories, hence there is need for the development of agrotechnology for cultivation in large scale. This species has the potential components for developing new molecules in future for drug development. The present communication provides traditional usages, phytochemistry, therapeutic potentials, and future perspectives of S. costus. This baseline information provided in this article will be helpful in future for research to be carried forward in drug discovery programme using this plant species.

With the sharp development of urban and industrial growth, the area under agriculture is declining year by year. On the other hand, human and animal population is increasing at a very fast rate. So, to meet the growing demand of food and fodders, a vertical increase in yield is required. Crop productivity per unit area can be enhanced with the manipulations of some agronomist practices such as sowing the crops at appropriate time, selection of suitable field for raising a crop, adoption of best planting techniques, use of appropriate seed rates, adoption of proper irrigation schedule, proper and need based application of fertilizers, adoption of zero till technology, green manuring, mulching, addition of well decomposed farm yard manure, timely and proper weed management, selection of recommended crops and crop varieties, proper control of insects and pests, adoption of marketing tips etc.

Tomato

Tomato, a warm season vegetable, requires a long season to produce a profitable crop. The optimum range of temperature for its cultivation should be 21–24ºC. The mean temperatures below 16ºC and above 27ºC are not desirable. The minimum, optimum and maximum soil temperatures for seed germination are 10º, 30º and 35ºC, respectively. Maximum seed germination occurs at 24ºC. Maximum fruit setting occurs at night temperature of 15-20ºC. Flowers fail to set fruit below 13ºC and beyond 38ºC. Formation of red colour due to lycopene is inhibited when temperature exceeds 32ºC. Lycopene is highest at 21-24ºC, while its production drops off rapidly above 27ºC. Tomato plants are highly susceptible to high rainfall areas if proper drainage is not available.

It is grown in many types of soil from sandy to heavy clay. A well-drained fairly fertile loam soil with a fair moisture-holding capacity is ideal for growing its good crop. It prefers a soil having a pH of 6.0 - 7.0. Application of liming is beneficial if it is cultivated in acid soils.

It can be grown almost round the year. The planting seasons vary from place to place. In northern India, for spring summer crop, it is sown in November and transplanted in second fortnight of January. In area, where frost does occur, it is sown in July-August and transplanted in August-September. In hilly areas, it is sown in March-April and transplanted in April-May.

• Harmony is the principle of landscaping.

• Texture is an important element of landscaping.

• Blue is a primary colour.

• Green in a secondary colour.

• Garden furniture is a tangible item.

Indication

Necrosis, Gangrene, Extensive injury of nerve of hind limb.

Malignant neoplasm

Frost bite. Burns

Incurable peripheral vascular disease.

Hip joint is a ball and socket joint which involves cotyloid cavity of os-coxae and head of femur. Hip dislocation can be corrected by external immobilization of the area with POP cast and keeping the animal in a mobile sling for several weeks.

The dislocation involves cotyloid cavity and head of femur and the affected limb deviate outward. (Fig.1)

The fracture site is immobilized extending joint below and joint above level initially with plaster of Paris.(Fig.2)

Cloth bandage is applied over it for support involving joint above and joint below which should not be too tight or too loose.(Fig.3)

Second layer of cloth padding is provided over the first layer to provide more strength and further immobilization.(Fig.4)

Final immobilization is done by application of rubber belt or suspensory bandage. (Fig.5)

At the end the entire animal is kept in mobile sling for proper immobilization for some weeks and housed in limited space(Fig.6)

Histiocytoma is more common in younger dogs below two years of age. It is also observed in all age groups.

Gross examination

Grossly, histiocytoma appears as light pink, smooth to firm, raised round (Figure 77) masses. Alopecia and ulceration are also observed.

McManus’ Method for Glycogen (PAS)
Tissues fix in 10% neutral buffered formalin and process for routine paraffin sections
Protocol
1) Deparaffinise the paraffin sections with two changes (10 minutes each) of xylene.
2) Descending grades of ethanol from absolute to 50% solution (2 minutes each).
3) Hydrate the sections with distilled water 5 minutes.
4) 0.5% Periodic acid solution for 5-6 minutes.
5) Rinsing in distilled water.
6) Schiff’s’s reagent or Coleman’s Feulgen solution for 10-15 minutes.
7) Washing in running tap water for minimum 10 minutes until pink colour develops.
8) Counter stain with Harris’ hematoxylin for 2-3 minutes to stain nuclei.
9) Washing in running water for 2-3 minutes.
10) Quick dip in ammonia water to develop blue colour of the nuclei.
11) Washing in running tap water for 5 minutes.
12) Dehydrate in ascending grades of 90% to absolute ethanol (one minute each).
13) Clearing in xylene, two changes of 30 minutes each.

1. Introduction

Human activities significantly lead to deterioration of ecological systems, affect individual organisms, and disrupt natural ecosystems. The rise in foreign compounds, such as heavy metals and pesticides, in aquatic ecosystems due to human activities has spurred extensive global research efforts (Wester et al., 2002). The Union’s Water Framework Directive (WFD) mandates monitoring programs to assess water body health. In environmental studies, understanding the impact of contaminants on aquatic life requires a multifaceted approach (Ullah and Zorriehzahra, 2015). Biomarkers that observe biological responses at various levels offer crucial insights into the presence and bioavailability of toxicants, complementing traditional methods (Yancheva et al., 2016). These indicators, termed early warning signals, are vital for evaluating exposure, effect mechanisms, susceptibility to contaminants, and enhancing predictive models (Yancheva et al., 2016). Integrating biomarkers into ecological studies provides a holistic understanding of environmental stressors and their implications in aquatic organisms.

2. Why is fish used as a model organism in ecotoxicological studies?

According to the Water Framework Directive (WFD), fish serves as a crucial indicator of river ecological status (Ullah and Zorriehzahra 2015). Their diverse sizes, ages, trophic levels, and sensitivity to various toxicants make them excellent indicators of water contamination (Wester et al., 2002). Fish adapt their metabolic functions in response to environmental changes, which makes them valuable in toxicological research. Monitoring sentinel fish species is a widely accepted method for assessing toxicant accumulation and its impact on human health (Wester et al., 2002). Fish, with their developed osmoregulatory, endocrine, nervous, and immune systems, are preferred to invertebrates in toxicological studies. Fish can absorb toxicants through both waterborne and dietary exposure, allowing the evaluation of contaminant transfer through the food chain. Key tissues for ecological, toxicological, and pathological studies in teleost fish include the gills, liver, and kidney because of their high metabolic activity and tendency to accumulate toxicants (Wester et al., 2002).

A protein that is deposited in various tissues of body called amyloid. Amyloidosis is a disorder of protein folding, in which normally soluble proteins accumulate in the tissues as abnormal insoluble fibrils (or filaments), thus disrupting their function The deposited proteins, generically known as amyloid, damage the structure and function of the tissues and so cause serious disease which is often fatal when it affects major organs. There are several techniques to observe the amyloid which is as follows.
Highman’s Congo Red Technique
Tissues fix in 10% neutral buffered formalin and process for routine paraffin sections The formal saline is a better choice
Protocol
1) Sections to water, removing pigment where necessary.
2) Stain in Congo red solution, 5 minutes.
3) Differentiate with the alcoholic potassium hydroxide solution, 3-10 seconds.
4) Wash in water, stain nuclei in alum hematoxylin and differentiate blue.
5) Dehydrate, clear, and mount.
Observations
Red : Amyloid, elastic fibers, eosinophil granules: Blue : Nuclei
Chemical reagents
Immerse in alkaline sodium chloride solution for 20 minutes.

Pituitary
Herlant (1960) Pituitary Stain I
Tissues fix in 10% neutral buffered formalin or Zenker formol and process for routine paraffin sections
Protocol
1) Deparaffinise the paraffin sections with two changes (10 minutes each) of xylene.
2) Descending grades of ethanol from absolute to 50% solution (2 minutes each).
3) Hydrate the sections with water 5 minutes (remove HgCl2 if present).
4) Stain in erythrosine for 5 minutes.
5) Rinse briefly in distilled water.
6) Stain in Mallory II for 5-10 minutes.
7) Rinse briefly in distilled water.
8) Stain in acid Alizarine blue for 5-10 minutes.
9) Rinse briefly in distilled water.
10) Treat with phosphormolybdic acid for 5-10 minutes.
11) Rinse briefly in distilled water.
12) Differentiate in 70% ethanol

Histomoniasis is a protozoan disease.

Etiology

Histomonas meleagridis.

Hosts

Mainly disease of turkey but has also been reported in chickens, peafowl, grouse, quail and gallinaceous birds.

Mammary tumours arise from the ducts are called as ductal or ductular carcinoma. Some include other tissues such as the myoepithelium and connective tissue between the glands. These have a number of different names including carcinosarcoma, carcinoma or sarcoma in benign tumour, complex adenocarcinoma and malignant mixed tumour. Tumours are also classified by the type of epithelial growth because this influences the probability of recurrence. Tumours that only have myoepithelium or connective tissue are called sarcomas.

• General considerations

 • Scope

 • Microscopic examination techniques

 • Smear

 • Vital staining

• Routine histopathological techniques of sectioning

 • Collection of tissues

 • Fixation

 • Washing

 • Dehydration

 • Clearing

 • Impregnation

 • Casting of blocks

 • Trimming

 • Section cutting

Histopathology is a critical diagnostic method used to examine tissue structure and detect pathological changes caused by infectious agents in fish. In India’s rapidly growing aquaculture sector—encompassing economically significant species such as rohu, catla, tilapia, pangasius, and ornamental fish— histopathology plays a pivotal role in disease surveillance, diagnosis, and research.
As fish farming intensifies across major aquaculture states like Andhra Pradesh, West Bengal, Tamil Nadu, and Kerala, advanced diagnostic techniques like histopathology are becoming increasingly important. These techniques enable early detection of diseases and support efforts in biosecurity and sustainable fish farming.
The process begins with the careful collection of tissues from fish suspected of disease. Key organs examined include gills, liver, kidney, spleen, heart, intestine, and muscle, which are commonly affected in systemic infections of bacterial, viral, parasitic, or fungal origin. Fish samples are collected from ponds, tanks, cages, or RAS units and transported under chilled conditions—preferably on ice—to laboratories such as ICAR-CIFE, state fisheries universities, or certified private centers.
Tissue dissection is conducted under aseptic conditions. Samples are immediately fixed in 10% neutral buffered formalin, the most commonly used fixative in India, to prevent autolysis and maintain cellular architecture. Fixation typically takes 24–48 hours, depending on tissue size and thickness.
After fixation, tissues are processed through graded alcohol dehydration, clearing in xylene (or substitutes), and embedding in paraffin wax. These steps are often performed using automated tissue processors available in regional laboratories. Once embedded, tissues are sectioned at 4–6 microns using a rotary microtome and mounted on clean slides.

  Term anesthesia by Dr Oliver Wendell Holmes (1886).
  Anasthesis (Greece Word) – absence of sensation.

History of Animal Anesthesia
  Paracelsus (1540) produced ether and reported it to have a soporific effect on fowl. 
  Sir Humphrey Davy (1800) suggested that N₂O might have anesthetic property. 
  Chloroform was discovered by Liebig in 1831.
  Dr. Horace Wells (1844) discovered the general anesthetic properties of nitrous oxide. 

Surgery : The branch of medical science which deals with the treatment of injuries, deformities or disease condition by manipulation or operations with the hand. The term surgery is derived from a Greek word – Chirurgia. 
Cheir – Hand 
Ergon – Work 
Veterinary surgery : Veterinary surgery is the surgery which is practiced on animals. 

INTRODUCTION

In biology, fertility is de?ned as capacity to produce off-springs; however, in Soil Science a ?ne difference is made between a fertile and a productive soil. Soil Productivity is the ability of a soil to produce a crop using the entire spectrum of its physical, chemical and biological attributes, while Soil Fertility refers to the capacity of a soil to supply all essential plant nutrients in the desired amounts. Thus, soil fertility management is basically the nutrient management. To put it in simple words, a productive soil is always fertile, while a fertile soil may not be productive for a variety of factors, such as lack of adequate moisture in drier regions or lack of adequate air due to water-logging for most upland crops. Of late, a term ‘ Soil Health’ has been introduced. Soil Health is an assemblage of chemical, physical and biological parameters that closely relate to native or acquired production capacity and sustenance of ecologically important regulatory role (Katyal et al., 2016). It is measured against prede?ned parameters of soil’s status of fertility, compact ability, erodibility and biology. This again tends to relate soil fertility to nutrient content, availability and management.

Introduction

Farming system is complicated inter-related with soil, plants, animals, implements, power, labour, capital and alternative inputs controlled partly by farming families and influenced to varied degrees by political, economic, institutional and social forces that operate at several levels. The farm is viewed during a holistic manner. The farmers are subjected to several socio-economic, biophysical, institutional, body and technological constraints. Farming system conceptually may be a set of components or parts that are reticulate that act amongst themselves. At the centre of the interaction is that the farmer exercise management and selection concerning the sort and results of interaction. It’s the human setting that gives ample condition for development and utilization of a specific system. A farming system clearly is incredibly complicated system. Therefore, any agricultural technology compatible to a specific agroecological state of affairs and socio-economic environment might not be adopted by alternative farmers. Unless each natural and human environment is thought-about, agricultural analysis won’t end in relevant agricultural technology. The income from cropping alone in tiny and marginal farms is hardly ample to sustain the farmers’ family. With the decline in farm size (0.15 ha) because of explosion of population, it’d be more and more tough to provide enough food for the family by the tip of the century. Therefore, the farmer, to be assured of an everyday income for a good living (above the poverty line), a considered mixture of anybody or additional of those enterprises with agronomic crops ought to complement the farm income and facilitate in usage the farm residue/waste. The choice of enterprises should be supported the cardinal principle of minimizing the competition and maximizing the complementarily between the enterprises.