eChapters

1.1. Dry farming (Dryland farming)

Cultivation of crops in a region where the annual rainfall is more than 750 mm.

Semi arid crops with growing period of 75-120 days.

Crop failure is less frequent.

Moisture conservation practices are necessary.

14.1. Soil fertility

Ability of the soil to supply essential plant nutrients during growth period of the plants

Crop producing capacity of the soil

13.1. Soil survey and soil mapping

Refers to study and mapping of soils in the fields.

Process of classifying soil types and other soil properties in a given area and geo-encoding such information.

Draws information on geomorphology, theories of soil formation, physical geography, analysis of vegetation and land use patterns.

Primary data for the soil survey are acquired by field sampling and by remote sensing.

Remote sensing principally uses aerial photography (Illuminating the object/ target with pulsed laser light and measuring the reflected pulses with sensor), but LiDAR ( Light Detection and Ranging) and other digital techniques.

12.1. Problem soils

Chemical constraints: Salinity, sodicity, acidity and nutrient toxicities

Physical constraints : High or low permeability, sub soil hard pan, surface crusting, fluffy paddy soils, sandy soils etc.

Word manure derived from French ‘Manoeuvrer, means to manipulate, to work, to produce crop.

11.1. Sources of organic wastes

Cattle shed wastes, dung, urine and slurry from biogas plants.

Human habitation wastes-night soil, human urine, town refuse, sewage, sludge and sullage.

Poultry jitter, droppings of sheep and goat.

Slaughterhouse wastes-bone meal, meat meal, blood meal, horn and hoof meal, fish wastes.

Byproducts of agro industries-oil cakes, bagasse and press mud, fruit and vegetable processing wastes etc.

Any material of natural or synthetic origin added to the soil to supply one or more plant nutrients.

10.1. Classification of fertilizers

10.1.1. Straight fertilizers

Supply only one primary plant nutrient, namely nitrogen or phosphorus or potassium.

Eg. Urea, ammonium sulphate, potassium chloride and potassium sulphate.

9.1. Nutrient deficiency of corrective measures

9.1.1. Major and secondary nutrients

8.1. Classification of essential nutrients

8.1.1. Based on the amount required by the plant

Major nutrients-Required in large quantities eg. N, P, K.

Secondary nutrients-Required in lesser quantities compared to major nutrients eg. Ca, Mg, S.

Micronutrients-Required in trace quantities eg. Fe, Mn, Zn, Cu, B, Mo.

Involved in plant metabolic functions and the plant cannot complete its life cycle without the element.

7.1. Plant nutrient concentration and plant growth

1. Deficient

Lowest concentration of an essential element that limit yield severely and cause distinct deficiency symptoms.

6.1. Humus

Fraction of organic matter.

Complex and resistant mixture of brown or dark brown amorphous and colloidal organic substance.

6.2. Humus formation

1. Decomposition: Chemicals in the plant residues are broken down by soil microbes.

Simpler chemicals are metabolized into new compounds in the body tissue of soil microbes.

New compounds are subjected to further modification and synthesis as the microbial tissue is subsequently attacked by other soil microbes.

5.1. Organic matter

In sandy soil, the sticky and slimy organic materials cement the sand particles together to form aggregates.

In clayey soil, it modifies the properties of clay by reducing its cohesiveness and making clay more crumby.

4.1. Role of soil microorganisms

Soil N utilization by plants.

Dead organic matters decomposed.

Nutrient transformation.

Physical and chemical properties of soil.

CO₂ content.

Soil formation.

3.1. Soil colloids

Clay fraction of the soil contains particles less than 0.002 mm in size.

Particles less than 0.001 mm size possess colloidal properties and are known as soil colloids.

2.1. Soil structure

Important physical properties of soils

Soil texture

Soil structure

Surface area

Soil density

1.1. Definition

Soil: Thin layer of earth’s crust made up of disintegrated and decomposed rocks, complex mineral compound, organic matter, water/air and living organism like bacteria, fungi, insects and worms and serves as the natural medium of growth of plants.

Provides nutrients, moisture, anchorage (support) and air to plants.

Parent materials are igneous rocks, sedimentary rocks and metamorphic rocks.

Soil Science: Science dealing with soil (Natural resource) on the surface of the earth, including pedology (Soil genesis, classification and mapping), physical, chemical, biological and fertility properties and their relation to management for crop production.

Arid regions lie under the category of wastelands which are not being used to their fullest potential therefore, it almost barren. Hot arid regions are sparsely populated with human and animal population and experiences various biotic exploitation of the already very poor resources base. The scarcity of water poses biggest hindrance in limits the crop productivity as climatic factor. Yet, nature has bestowed the arid region fruits with characteristic qualities. Some fruit crops can grow successfully in such type of regions. Among these crops, cardia most suitable crop for cultivation under such type of arid climate. Some regional names of cordia are Pidar, Ponugeri, Maruvilli, Geduri, Spistan, Bhakar, Borla, Kalo Bohori (Nepali) etc. It is growing not only all over Asia but also nearly whole of the globe especially in tropical environment. In Indian sub-continent, it is growing from east Myanmar to west Afghanistan. It is grown in arid region and having numerous utilities. It is found in a variety of the forest of Rajasthan to moist deciduous forests of the Western Ghats and Tidal forest of Myanmar. In Maharashtra, it grows in moist monsoon forests also. In Rajasthan, its commercial plantations are being done in Pali, Sirohi, Udaipur, Jodhpur and Jalore districts.

Now, it is becoming popular as monoculture cultivation as well as under agroforestry system in arid region. It does not grow gregariously but is found growing singly in moist shady ravines and valleys. In areas with annual rainfall less than 500mm, it thrives along streams and depressions where moisture is available. The hot arid zone stretch 32 M ha or 0.32 million square kilometer long covering Rajasthan (62%), Gujrat (20%), Andra Pradesh (7%), Punjab (5%), Haryana (4%), Karnataka (3%) and Maharastra (0.4%). In these area fruits selection for cultivation must be cope up that their maximal growth period coincides with the period of water availability in the soil. Few of the xerophytes characteristics are: deeper root system, deciduous nature, reduced foliage, sunken of covered stomata, waxy coating or hairiness on leaf surface which minimize the evapotranspiration and makes plant adaptive to the hostile climate. These qualities are found in cordia plant which can grow in this area without any problem after management of some agro-techniques.

Ker (Capparis decidua (Forsk.) Edgew, (synonym Capparis aphylla, Roth) is a multipurpose, perennial, woody shrub or small tree found growing naturally in hot arid regions and semi-arid subtropics in various parts of the world. In India, it is known by different names in different parts of the country. In Rajasthan, it is generally called Kair or Ker while in Haryana it is known as Teent or Dela. Some more synonym by which ker is known are Caper, Karyal, Hanbag, Cari, Karira etc while in English, it is known as Caper berry. This species is an important constituent of arid ecosystem and plays significant roles in sustaining the ecosystem. The peoples of Indian Thar desert have known the importance of this shrub for long back. On account of its acquired xerophytic adaptive characteristics, it is capable of growing in various kinds of habitats including wastelands. It is also suitable for stabilizing sand dunes and controlling soil erosion by wind and water. Due to its xerophytic adaptive nature, the plant grows successfully under harsh climatic conditions of arid regions. Berry-shaped unripe fruits are rich in carbohydrates, proteins and minerals. It was abundant about five decades back, but, with increase in populations of humans and livestock and more demand of land for agricultural purpose, it has been over-exploited and eliminated by tractor ploughing and excessive browsing pressure from livestock. This has resulted in significant reduction in plant population of ker, but people still try to retain some plants on farm boundaries as this also prevent soil erosion which is quite high due to wind.

Karonda (Carissa carandas Linn.) is also known as ‘Christ Thorn Tree’ and an important fruit plant of semi-arid and arid regions. It is an evergreen, hardy, spiny bush which is use as live fencing surrounding the orchards or filed. One or two plant was planted in backyards at rural place. It is also grown in wasteland, problematic soil, unproductive land and low moisture conditions. It can be also grown as ornamental purpose due to pink reddish fruits and evergreen nature. Plant produce sufficient amount of fruits and fruits are sour and astringent in taste. Fruits havenutritive value like rich source of iron, fair amount of vit. C and several medicinal values.

Wood apple is also known as kaith, kavat, curd apple and monkey fruit. It is found almost throughout the country. It is one of the very hardy tree found growing in arid and semi arid regions of the country. Hill region of northern India and Ceylon are supposed to be its native land (Reuther et al., 1967). Chundawat (1990) thought it to be a native of India and Sri Lanka. It is a popular tree of forest, Indoor yard garden and can be exploited for regular cultivation in dry areas. The cricket ball size fruits have sweet and aromatic pulp, eaten fresh or used for drinks, chutneys, jellies etc. In forests of Rajasthan, it is commonly found in Aravalli range and Hadoti Plateau.

Tamarind (Tamarindus indica L.) is a tropical evergreen tree species of Leguminosae family. It is a very versatile plant species which can grow easily up to 1500 m above sea level and found in abundance in the Indian subcontinent and African continent. The name Tamarindus indica was given by Linnaeus in the eighteenth century, inspired by the Arabic name “Tamar-ul-Hind”, meaning ‘date of India’. Tamarind is known by different vernacular names in different languages. In India, tamarind grows naturally in forest and also develops unattended on wastelands. Therefore, it is suitable to grow along the roads, in agroforestry as multipurpose tree species or as fire breaks for forest margins.

Phalsa is an important minor fruit crop of India. It is a sub tropical fruits, which is also known as star apple. It is known by different vernacular names in different parts of country for example Dhamini in Bengali, Phalsa in Punjabi and Shurkhi in Hindi.

Phalsa is a shrub or small tree of Indian origin. Phalsa has been mentioned in Vedic literature as having certain medicinal qualities. Phalsa is a bushy plant of arid and semi- arid region. It is also cultivated in subtropical region. It is very hardy, tolerant to drought and suitable to grow on wastelands. Phalsa is best suited for small area as it is manageable by pruning. Its cultivation is favoured around big cities where fruits find a ready and quick sale. It has a poor keeping quality. It is also grown as an intercrop with mango, aonla, bael and ber. It is capable of growing under neglected and water scarcity conditions where only a few other crops would survive.

This fruit plant is fast-growing when young, but soon become slow-growing tree thrive well under tropical, sub-tropical and temperate region. Mostly this crop is being cultivated for rearing of silkworm and wind break rather than fruit crop. This crop not specific with climatic requirement so can be grown in arid, marginal soil, barren land without much care.

The different plant parts viz., stem, bark, twigs, leaf, fruit etc are being utilized in different ways. Stem use for making agricultural implement, house artisans’, sports items, fodder for animals and fruits use as fresh dessert and in processing for making of different value added products like jam, beverages etc. Apart from these, its different plant parts also have medicinal properties use mainly in folk, ayurvedic and unani medicines.

Jackfruit (Artocarpus heterophyllus Lam., Syn., Artocarpous integrifolia L.) is an evergreen tree species. Breadfruit (Artocarpus altilis) is a close relative of jackfruit. It belongs to Moraceae family along with fig and mulberry. It is a tree borne fruit crop species producing biggest size fruit among fruit crops. Jackfruit is the national fruit of Bangladesh and Indonesia (Matin, 2015). In southern India, Jackfruit received a respected status symbol. The state government of Kerala declared it as the state fruit of Kerala to promote the ‘Kerala Jack fruit’ and its value-added products as a brand, highlighting its organic and nutritious qualities among people.

The Jamun (Syzygium cuminii Skeels) is a nutritious fruit with a variety of uses; virtually every part of the tree has been utilized by both urban and rural dwellers. The fruit is good source of iron, sugars, minerals, protein and carbohydrate etc (Table 1). Fully ripe fruits are eaten fresh and can be processed into beverages like jelly, jam, squash, wine, vinegar and pickles. The Jamun fruit has sub-acid spicy flavour and squash is very refreshing drink for quenching the thrust in the summer season. A little quantity of fruit syrup is much useful for curing the diarrhoea. The vinegar prepared from juice extracted from slightly unripe fruit is stomachic, carminative and diuretic, apart from having cooling and digestive properties (Thaper, 1958). However, small jamun fruits not suitable for table use but suitable for use in the beverage industry as they contained a high amount of acidity, tannins and anthocyanins (Anon., 1986). The volatile oil from the jamun fruits can also be extracted (Vijayanandet al., 2001). Fruits are used as an effective medicine against diabetes, heart and liver trouble (Singh, 2001). The Powdered seeds have also reputation of being useful in the treatment of diabetes. Oral administration of an aqueous jamun seed extract for 6 weeks caused a significant decrease in lipids, thiobarbituric acid reactive substances and an increase in catalase and superoxide dismutase in the brain of diabetic rats (Prince et al., 2003). Leaf extract of jamun also reduces the radiation induced DNA damage in the cultured human peripheral blood lymphocytes. Jamun seed can be used as concentrate for animals, as it is rich in protein, carbohydrates and calcium. Its timber wood is used for railway sleepers, construction of buildings, agricultural implements and for well work, as it resists the action of water (Chundawat, 1990).

Pineapple (Ananas comosus L.) is an important commercial fruit crop belongs to family Bromeliaceae. It is one of the choicest fruits all over the world because of its characteristic taste and pleasant flavour. Plant is herbaceous in nature with narrow pointed leaves and 50–100 cm height. The inflorescence composed of 150–200 flowers arranged in a compact spiral cluster. The flowers are perfect with three short fleshy sepals and petals, six stamens, floral bract and ovary with three locules. The plant bears a single fruit of cylindrical shape terminally on a peduncle come out from the centre of the rosette. Pineapple fruit is a multiple or sorosis type fruit formed by the partial fusion of numerous fleshy segments from several separate flowers and edible part of fruit mainly consists of the ovaries, bases of sepals, bracts and cortex of the axis. Commercial varieties are self sterile but easily crossable with plants outside their varietal group. The apex of fruit bears a compressed, leafy and spiny shoot known as crown. The fruit weight ranged from 1.00–2.50 kg and has outer colour yellow and very golden yellow flesh inside. Pineapple fruits are mainly consumed as fresh fruit, juice purpose and also used for canning. The typically yellow fruit flesh is found suitable for fresh consumption when sweet (10 to 18% sugar) and moderately acid (0.5 to 1.6%). Fruits are processed in various value added products like pineapple chunks, crushed pineapple, diced pineapple, slices, juices, syrups, jams, etc. The pineapple possess crassulacean acid metabolism (CAM), a photosynthetic carbon assimilation pathway with high water-use efficiency

The bael (Aegle marmelos (L.) Corr.) is indigenous to India and it is also called as Baelpatra, Bengal quince (John and Stevenson, 1979), Indian quince, golden apple, holy fruit, bel, bilwa, bela, maredoo and other dialectal names in India. Hindus used the leaves to worship Lord Shiva (Bilwa Patra) and keep them on Shivling and they consider it is very auspicious and to get the blessings of Shiva. The fruits form a vital ingredient of holy offerings to the God and Goddess through holy pyre (haven). In history, the mention of bael tree is traced to Vedic times (C 2000 BC. to C 800 B..) within the Yajur Veda.

Custard apple (Sitaphal) is one of the most delicious fruit. The genus name, ‘Annona’ is comes from the Latin word ‘anon’, which means ‘yearly produce’ has three species namely Annona squamosa, Annona cherimola and Annona reticulate. Out of which Annona squamosa has been extensively used as fruit crop and traditional medicine for different ailments. The seeds and the leaves also are being used for preparation of medicine. It is hardy, tolerant to drought, salinity and saline irrigation water to certain extent. It grows very well in shallow soil profile. It also sheds off leaves during stress condition to evade moisture loss from plant tissue through transpiration. This crop is known by varied name like sitaphal, shariffa and sugar apple.

Origin and Distribution

The custard apple is believed to be a native of the West Indies but it was carried in early times through Central America to southern Mexico. It has long been cultivated and naturalized as far south as Peru and Brazil. It is commonly grown in the Bahamas and occasionally in Bermuda and southern Florida. Apparently it was introduced into tropical Africa early in the 17th century and it is grown in South Africa as a dooryard fruit tree. In India the tree is cultivated, especially around Calcutta, and runs wild in many areas.

Litchi (Litchi chinensis Sonn.) is a subtropical and evergreen tree belonging to the family Sapindaceae. This fruit has highly specific climatic adaptability due to chilling requirement for floral initiation on one hand and the damage caused due to high summer temperature along with low relative humidity during fruit growth period on the other hand. Some other factors influencing expansion in different regions of the world are difficulties in establishment of plants in nursery and orchards, bearing and further into harvesting and post-harvest management. Calcium rich soil with mycorrhizae is best suited for the luxuriant growth of litchi plants which are commercially propagated by air-layering or gootee.

There has been a substantial increase in the area and production of litchi in India during the past 50 years. The area has increased from 9400 ha (1949–1950) to 91,000 ha (2016–2017). The contribution of litchi to the total area under fruit cultivation has increased from 0.75% to 1.70%. Increase in area during 1991–1992 to 2016–2017 (22 years) has been more than 80%, whereas production increase during the same period is to the tune of more than 150% (NHB, 2016–17). Productivity also recorded an increase of about 50% during the same period. Evidently, production and productivity of litchi are constantly increasing in the country. On a global scale, the fresh fruit market dominates the trade, followed by dried and canned fruit. Low success rate of establishment, lesser fruiting span, low and irregular yields because of poor flowering and fruit set, fruit cracking, browning and rotting of fruits, fruit borers and mites, poor shelf life, and lack of suitable varieties with early and late maturity and good-quality fruits are some of the factors encountered

Date palm (Phoenix dactylifera L.) is an important fruit tree of semi-arid and arid regions. It grows well under poor desertic soils due to its hardy plant characteristics and deep root system. It is also one of the most suitable fruit trees, which can be easily grown under saline conditions. In arid region, crop production is risky preposition, where date palm cultivation contributes in achieving food security, high nutritive value food, crop diversification, desertification control, income generation and foreign exchange earnings. It can be grown at such places where adequate irrigation facilities are available besides other climatic requirements.

The latin name of the date palm, Phoenix dactylifera is derived from a Phoenician name “Phoenix”, which means date palm and dactylifera derived from a Greek word “daktulos” means a finger. In India, generally two species of Phoenix are found. Out of which, Phoenix sylvestris Roxb. called “desi khajoor” is found throughout the country but it produces inferior quality fruits. The fruit contains large stone with less pulp content. From its crown parts, a liquid is extracted known as neera or taddi, which is used as fresh drinks and also for preparation of jaggary. The tree of wild date/ desi khajoor (Phoenix sylvestris) is found in Bundelkhand region of the country and it is suitable tree component for agro forestry system (Tewari et al., 2001). Phoenix dactylifera is commercially grown for edible fruits and value added products.

The ber is an important indigenous fruit and growing in semi arid conditions in the country. Ber is grown in China for the last 4000 years and under sub tropical to tropical as well as Mediterranean regions of the world. It is cultivated widely for its resistance to grow in drought and other diversified climatic and soil conditions, so it is also known as ‘King of fruit’ of arid.

Origin and Distribution

The origin of ber is said to be India to South-western Asia. The ber is being cultivated in Northern India, central India and peninsular India. Hisar, Rohtak, Panipat, Gurgaon, Jind, Mahindergarh in Haryana. In Rajasthan it is cultivated in district of Bharatpur Alwer, Barmer, Jodhpur, Palib and Gnaganagar Jaipur in area 82, 73, 59, 53, 53, 53,41 hectare with production 365, 395,133, 294, 340, 597, 826 metric tones, respectively. However total cultivated area in Rajasthan is 738 hectare with production 5248 metic tones under ber during 2017–18 (Anonymous, 2018).

The area Sangrur and Patiala in Punjab; Banaskantha and Sabarmati in Gujarat; Bijapur and Bellary in Karnataka; Tirunalveli, Ramanathapuram and Salem in Tamil Nadu are major ber growing areas in the country. Besides India, ber is grown in Afghanistan, Iran, Syria, Burma, Australia, USA, Russia, Italy, France, Spain and Northern Africa.

Medicinal plants especially underutilized fruit are the nature’s gift to arid zone. Many of fruits are popular in this area but aonla posses an important place among all. Aonla or Indian gooseberry (Emblica officinalis Gaertn) is native to tropical south-eastern Asia mainly peninsular of central and southern India. The tree is known as Amla, Amlaki, Amli, Ambala, Amalakamu and Nelli in different parts of India. It is a very hardy tree and can be grown under diverse soil and agro-climatic conditions without much care. Aonla fruits has been reported to have potential of antioxidant, free radical scavenging capacity, anti-inflammatory, anti-mutagenic, immune modulation properties, which is used to cure various cancer, diabetes, liver and heart related diseases

Aonla has been regarded as a sacred tree in India. It has religious value and it is medicinally very important since ancient time. It has hallowed position in Indian medicine system called as Ayurveda as a protective remedy for several mankind problems. The tree was worshipped as Mother Earth and is believed to nurture humankind because the fruit are very nourishing. Kartik Mahatma and Vrat Kaumudi order the worship of this tree. The leaves are offered to the Lord of Shri Satyanarayana Vrata, Samba on Shri Shanipradosha Vrata and Shiva and Gowri on Nitya Somvara Vrata. The fruit and flowers are also used in worship. Fruits of aonla are processed in various forms like Juice, Murabba, Chayvanprash, candy, powder and flakes which has significant role in mitigating several malnourished disorders. Aonla cultivation has gained special attention of researcher community due to its significant role in medicinal industry as well as processing potential.

It is tropical fruit which is commercially cultivated in humid areas of India. Fruits are very tasty, sweet and rich in sugar. Trees are ever light green, slow growing and it attains a height of about eight meters.

Origin and Distribution

Sapota is native to Central and South America, specifically from the Yucatan Peninsula of Mexico to Costa Rica, where the largest population of native trees still exists (Gilly 1943). It is now widespread throughout the tropical regions of the world, including Central and South America, the West Indies, India, and Florida in the United States.

Sapota is grown on a commercial basis in India, the Philippines, Sri Lanka, Malaysia, Mexico, Venezuela, Guatemala, and some other Central American countries. India is the largest producer of sapota fruit with current production around 24,000 ha (Chadha 1992). Sapota is widely planted in south Florida, where the fruit is marketed locally and shipped to northern and eastern U.S. markets. The fruit, however, is not commonly seen in the United States. In southern Mexico and Central America where sapodilla is native, it is considered to be one of the best of the tropical fruits.

Pomegranate is one of the important fruits of tropical and sub tropical regions. It is also cultivated in semi arid and arid tracts of the country but requires irrigation facilities for better quality of fruits production. Pomegranate is used as fresh and making delicious juice. In India, it is cultivated for domestic consumption and export. Versatile adaptability, hardy nature, less cost in orchard management, low irrigation water requirement as compared to other commercial fruit crops, high yield potential, fine table and therapeutical values and excellent keeping quality has made this fruit more lucrative and remunerative (Sharma et al., 2014).

In global market, India plays a significant role of export of fruits. Recently exports of pomegranate from India have increased and extended to several countries. India stands firmly at third position with 14% market share in pomegranate export after China and Iran.

The annual export of pomegranate fruits from India is about 67, 892 metric tonnes (MT) amounting to revenue of ‘688 crore (Annon, 2019). For export purpose, Phule Arakta, Bhagawa, Super Bhagawa and Mridula varieties are suitable (Anon, 2003).

The highest importer of Indian pomegranate is Gulf countries led by United Arab Emirates (19,000 MT) followed by South East Asian Countries prominently, Bangladesh (10,500 MT) and Nepal (10,000 MT). Among European countries the biggest exporter is Netherlands (4,449 MT) followed by UK (576 MT). Recently, India has also started exporting fresh pomegranate to USA to the tune of 209 MT generating a revenue of 11 crore ((Annon, 2019).

Grape was known to grow in India from ancient time but its commercialization is relatively recent, with the discovery of, Anab-e-Shahi’ variety by Mr. Shanker Pillay in 1930 from the bungalow of Nawab Baquer Ali Khan. This variety performed very well in the Hyderabad region of Andhra Pradesh and in fact gave initial boost to the Indian viticulture. It was initially known as ‘Malta’ and proved to be a pioneer in revolutionizing grape industry in south Indian.

Grape growing in north India received impetus from its success in south Indian. The commercial grape growing in north India dates back to early sixties when the ‘Pusa Seedless’ variety was released by the Indian Agricultural Research Institute, New Delhi. Latter on, the Punjab Government imported 100,000 cuttings of early-maturing variety ‘Perlette’ from California (USA). This variety acclimatized well and proved good for commercial cultivation.

In western Rajasthan the grape was introduced around 1960, which became popular among the growers. The fruit yield declined slowly thereafter and the grape cultivation was almost abandoned by 1971–72, particularly in the arid district. The problem of the drop of flower and flower-bud has been over of the major constraints in grape growing in western Rajasthan.

Guava is the fifth most important fruit crop of India in area and production after mango, banana, citrus and papaya and also fifth most important fruit crop of India in area after mango, banana, citrus and apple (Anonymous, 2018). It is also called apple of tropics because it is sold at moderate prices. It is widely grown in different part of the tropics and subtropics. It is quite hardy, prolific bearer and highly remunerative crop which requires less care. Guava is an important fruit crop of India and has gained considerable importance because of its high nutritive value, availability at moderate price, a pleasant aroma and good flavour. Guava is one of the most common fruit liked alike by both the rich and the poor. Guava is a rich source of vitamin C and pectin. It is very rich and cheap source of vitamin C. The fruit is relished when mature or ripe and freshly plucked from the tree. It is also used for many preparations like jelly, cheese and juice.

Origin and Distribution

The world leading producer of Guava is India. It contributes 40.4% production of guava followed by china with 10.0% production. It is cultivated in India since early the 17^th century. Its origin is Tropical America. It has been spread throughout the tropics and subtropics. Guava is also cultivated in Israel but it is not common in the Mediterranean area and United States. However, Guava is growing widely in Southern Asia. It is cultivated in Ceylon at altitude of 1500 m and it also grown throughout Myanamar. In Florida, the first commercial guava planting was established around 1912 in Palma Sola. It is common throughout all warm areas of tropical America and in the West Indies (since 1526), the Bahamas, Bermuda and southern Florida where it was reportedly introduced in 1847 and was common over more than half the State by 1886. Apparently it did not arrive in Hawaii until the early 1800’s. Now it occurs throughout the Pacific islands. Generally, it is a home fruit tree or planted in small groves, except in India where it is a major commercial resource. In 1972, Hawaii processed, for domestic use and export, more than 2,500 tons (2,274 MT) of guavas, over 90% from wild trees. During the period of high demand in World War II, the wild guava crop in Cuba was said to be 10,000 tons (9,000 MT), and over 6,500 tons (6,000 MT) of guava products were exported. Brazil’s modern guava industry is based on seeds of an Australian selection grown in the botanical garden of the Sao Paulo Railway Company at Tatu.

Papaya (Carica papaya L.) has emerged from the status of a home garden plant to that of commercial orchards and is popular with the farmers because it is one of the few fruits which fruits throughout the year, give quick and high returns, requires less area per tree and adapts itself to diverse soil and climatic conditions. It is an important fruit of tropical and mild subtropical regions of the country. Though India is the leading producer of this fruit, the productivity (43.27 MT/ha) is below it’s potentially (100 t/ ha). In India, Andhra Pradesh, Gujarat, Karnataka, Madhya Pradesh and Maharashtra are important centres of its production.

Origin and Distribution

Papaya is native to tropical America, from Southern Mexico through the Andes of South America. It was spread to the south by Indians, and throughout the Caribbean with Spanish exploration. It was spread in 16^th century to other parts of the world. The Spanish also carried it to Europe and the Pacific Islands. By the mid 17th century, papaya was distributed pan tropically. Papaya was introduced to Hawaii in the 1800s, and Hawaii remains the only state in the USA to produce papaya commercially. Now it is grown in all tropical and sub-tropical countries like Australia, Hawaii, Taiwan, Puerto Rico, Peru, Florida, Pakistan, Bangladesh and India. It was introduced in India in 16^th century by Portuguese. There are leading producer countries in the world which are India, Brazil, Mexico, Indonesia, Dominican republic, Nigeria, Democratic republic of the congo, Cuba, Thailand and Venezuela (Bolivarian republic).

India, is the worldwide leading producer of papaya and its principal exportation market is focused on Gulf countries. The papaya cultivation in India was 138 with production 5989 (‘000 hectare production ‘000 metric tones) in 2017–18 and it was 146 with production 6096 (‘000 hectare production ‘000 metric tones) 2018–2019. At harvested area rank, Mexico is the fifth papaya harvester, with 17,500 hectares. The maximum area in Gujarat followed by Andhra Pradesh, Kerala, and Chhattisgarh; and production is maximum in Andhra Pradesh followed by Gujarat and Karnataka, Anony, 2019.

Citrus is a major fruit crop grown all over the tropical and subtropical region of the world. Maximum citrus species are native of tropical and subtropical regions of South East Asia specially India, China in the area between these two countries. It is mainly cultivated in China, Brazil, India, USA, Mexico, Spain and Egypt in the world. China is the leading producer country in the world followed by Brazil, India and USA. India is the third largest citrus producer which has 4.8 per cent of the world’s total citrus production.

Banana is an important fruit of India ranking second fruit next to mango. Its name “Adams fig”, “Apple of Paradise” and the botanical name M. paradisiaca are suggestive of its antiquity. The edible banana is indigenous to warm moist parts of Asia and probably originated somewhere in the mountainous hot tropical regions of South-East Asia (Assam, Burma, Thiland or Indo-China region). Bananas are a very important commodity for developing countries, with a clear dual nature which complicates the analysis. They are at the same time a major staple commodity, together with rice, wheat and maize, and a fundamental export commodity. Many issues are involved in order to make of banana a very sensitive commodity at the international level, not only on economical grounds but also on the environmental, social and political aspects.

Origin and History

It is believed that the earliest written reference to banana is in Sankrist and dates back to around 500 BC. Bananas are suspected to be the first fruit in the earth by some horticulturists. The origin of bananas is placed in Southeast Asia, in the jungles of Malaysia, Indonesia or Philippines, where so many varieties of wild bananas still grow at present. Bananas have later travelled with human population. The first Europeans to know about bananas were the armies of Alexander the Great, while they were campaigning in India in 327 BC. In the Middle Ages, the banana was thought to be the forbidden fruit of paradise by both Muslims and Christians. The Arabs brought them to Africa. Africans are credited to have given the present name, since the word banana would be derived from the Arab finger. The Portuguese brought them to the Canary Islands. Bananas changed during all these trips, gradually losing its seeds, filling out with flesh and diversifying. When Spaniards and Portuguese explorers went to the New World, the banana travelled with them. In 1516, when Fiar Tomas de Berlanga sailed to Santo Domingo, he brought banana roots with him. From there, bananas spread to the Caribbean and Latin American countries. Bananas started to be traded internationally by the end of XIX century.

Mango is most important of all fruit crops in terms of are coverage and production, besides its socioeconomics providing livelihood to million of people in India and it is acknowledged as the king of fruit of this country. The greatest tribute of this esteemed plant was paid by Emperor Akabar (1556–1605) who established the Lal Bag (a mango orchards having one lakh plants) in Darbhanga (Bihar) when large orchards of fruit trees were unknown. In Ain-I-Akbari, a master piece of Persian literature, written by the great chronicler Abul Fazal (1590 AD) during the region of Akbar. Popularity of mango among the consumers is increasing due to its delicious flavour and much higher nutritional and therapeutic values. Thus, mango is now appreciated and spreading across the globe. It is grown in more than 90 countries. Washing of mouth and hand are necessary after eating the fruit so, it is also known as bathroom fruit.

Area and Production

India is the single largest producer of fruit, accounting for 49.6 per cent of area and 49.1 per cent of the total world production. Mango occupies 23.13 million hectares area in India. Amongst the major mango-exporting countries India, Mexico, Brazil and Pakistan accounted for 3.5, 1.4 and 3.3 per cent of total area under mangoes in the world respectively. The productivity of mango is highest with 13.6 tonnes/ha in Brazil and 7.0 tonnes/ha in India which is much lower. India contributed about 45.7 per cent of total mango production (35.71 million tonnes) in the world, making it the most important mango producing country. Andhra Pradesh ranks first as it produces about 27 per cent of total mango production and Uttar Pradesh is ranked second in production (23.9%) with productivity of13 tonnes/ha. At present the varieties like ‘Alphonso’ grown in Maharashtra and Gujarat and ‘Dashehari’ in U.P. are exported to countries viz. Afghanistan, Bahrain, France, Kuwait, Malaysia, Nepal, Qatar, Singapore and U.K. Mango nectar and pulp has great scope to European and American market.

Horticulture is the science and art of production, improvement and utilization of fruits vegetable, ornamental, aromatical medicinal, spices and plantation crops. The word horticulture derived from latin words ‘Hortus’ means garden and culture means cultivation.

The horticulture science can subdivided into different following branches on the basis of product utilization and crop involved.

Pomology: This word is derived from latin word pomus ‘Fruit’ and Greek term logy ‘Science’. The branch deals with the cultivation of fruits called pomology.

Olericulture: It is the branch of horticulture which deals with cultivation of vegetable crops product of which is utilized for culinary purpose after cooking. Olericulture word is derived from the latin word oleris means pot herbs and the english word culture means the culture of succulent vegetables.

Plantation crops: Cultivation and processing of tropical crops grown on a large scale centered around a factory constitute the plantation crops e.g., tea, coffee, black pepper.

Spices crops: These crops are grown and used for providing fragrance and flavouring to various food stuffs e.g., cinnamon, clove, pepper, coriander etc.

Medicinal plants: These plants are rich source of alkaloids and steroids having prophylactic and curative properties for treatment in several diseases e.g., Aloe, Isabgol.

Aromatic plants: Aromatic plants are rich source of essential oil which is used for cosmetic industry e.g., Rose, lemon grass etc.

Entomopathogenic nematodes (EPN) in the family Steinernematidae and Heterorhabditidae have enormous potential to control insect pests of economic importance. Since, chemical pesticides pose environmental hazards, these nematodes have received considerable attention as potential bio-insecticides because of their wide host spectrum, active host seeking, killing the host within 48 h, easy mass production, storage and application and are environmentally safe. One of the important criteria for a successful biocontrol agent is formulation of nematodes into a stable product, which has played a significant role in commercialization of these biological control agents. Apart from formulation field application and desired control of insect pests decides the success of EPN.

Formulation of Entomopathogenic Nematodes

EPNs have been known since 1929 but they became commercially available only during 1990s.The first attempts at formulating EPN were initiated in 1979 but the shelf life was very limited (one month). Infective juveniles carried on moist substrates such as sponge, vermiculite and peat require continuous refrigeration to maintain their viability. While formulating nematodes, two things are necessary, a long shelf-life and easy application method. The simplest method is impregnating a moist substrate (such as sponge, peat, vermiculite, etc.) with nematodes. The sponge needs to be squeezed in water before application, to release the nematodes, whereas nematodes from other carriers could be applied directly to the soil. However, these formulations are labour-intensive, require continuous refrigeration and lack economy of scale and therefore can be used on small scale only.

Although the infective juveniles of entomopathogenic nematodes can be stored for several months in water in refrigerated bubbled tank, high cost and difficulties of maintaining quality preclude the deployment of this method. Therefore, nematodes are usually formulated into solid or semi-liquid substrates soon after they are produced.

Introduction

Agricultural production in India is constrained by number of biotic and abiotic factors. India is a tropical country and has a climate which is conducive for insect pests which are, one of the major constraints in agricultural production, processing and storage. Annual crop losses due to insect pests and diseases in India are estimated to be 18 percent of the agricultural output. Insect pests are widespread in all the agroclimatic zones and large quantities of insecticides are used to control these pests. Indiscriminate use of insecticides, lack of adequate knowledge on proper application techniques and dosages, and frequent use of broad spectrum insecticides are common in India. There is a need to identify suitable alternative methods for the management of insect pests and among them, biological control using viruses, bacteria, fungi and entomopathogenic nematodes is gaining greater attention world over.

Nematode families of Steinernematidae and Heterorhabditidae attracted most attention as they contain the entomopathogenic nematodes (EPN). Entomopathogenic nematodes are obligate parasites of insects that kill their hosts with the aid of bacteria (Xenorhabdus and Photorhabdus) carried in the nematode’s alimentary canal. The lethal obligate insect parasites are ubiquitously distributed without pathogenicity to mammals and beneficial non-target insects, and exempted from registration requirements. Currently there are about 98 valid EPN species belonging to Steinernema (79) and Heterorhabditis (19). Soil surveys conducted in divergent areas of Indian subcontinent have demonstrated high diversity of EPN across seasons, habitats, and geographic regions. There are several target pests in India that can be controlled with entomopathogenic nematodes and several workers have used EPN against cutworms, stem borer, white grubs, etc. under laboratory and field conditions and are compatible with existing IPM programmes.

Introduction

Entomopathogenic nematodes (EPNs) viz., Heterorhabditis, Neosteinernema and Steinernema have been recovered from many regions of the world and have been used commercially as biocontrol agents of insect pests. They are associated with mutualistic bacteria in the genus Xenorhabdus for Steinernematidae and Photorhabdus for Heterorhabditidae. The infective juvenile nematodes enter their host insect through natural openings (mouth, anus or spiracles), they release bacteria into the insect and cause rapid death of insect host (24-48 h). EPNs were used against many insect pests viz., rice leaf folder, Cnaphalocrocis medinalis (Srinivas and Prasad, 1991); tobacco cutworm, Spodoptera litura (Rajkumar et al., 2003); brinjal fruit borer, Leucinodes orbonalis (Hussaini et al., 2002) diamond back moth, Plutella xylostella (Singh and Shinde, 2002); Diaprepes abbreviates (Jenkins et al., 2007); Bemesia tabaci (QiuBaoli et al., 2008) and sugar beet beetle (Saleh et al. 2009).

I. In vivo culturing technique

In vivo culturing means culturing of entomopathogenic nematodes (EPN) in the host. Generally rice meal moth, Corcyra cephalonica and greater wax moth, Galleria mellonella are used as insect hosts for rearing EPNs.Insect hosts such as Spodoptera, Helicoverpa and Bombyx mori are also used for culturing Steinernema and Heterorhabditis but these insects need plant host especially leaves for culturing. Corcyra can be grown in broken cumbu grains and Galleria in honey comb or artificial diet.

Basically we need to mount and prepare the slide for observation of various stages of EPN as detailed below in Table.1

Although the Rhabditids have been studied from last one century, the field level use of Entomopathogenic nematodes the members of Rhabditids for the management of insect pest was followed only after Dutky who practically used EPNs in 1937 on Apple Codling moth.

The taxonomy of Entomopathogenic nematodes of the order Rhabditidae is discussed in this lecture.

Introduction

Corcyra cephalonica commonly called as rice meal moth or rice moth is a pest of stored foods, viz., cereals, cereal products, oilseeds, pulses, dried fruits, nuts and spices. Many of the natural enemies mass-bred in the laboratory for use in field against crop pests are dependent on either egg or larval stages of Corcyra due to the simple reason that it is easier and cheaper to produce natural enemies on different stages of Corcyra than on their original hosts.

Morphology and Biology of Corcyra

The eggs are oval and measure 0.5 x 0.3 mm. The white surface is sculptured and has a short nipple-like structure at one end. The larvae are generally creamish – white except for the head capsule and the prothoracic tergite, which are brown. There are well-developed prolegs on abdominal segments 3-6 and 10. A fully matured larva measures 15 mm. The last-instar larva spins a closely woven, very tough, double-layered cocoon in which it develops into a dark-brown pupa. The anterior portion of the cocoon has a line of weakness through which the adult emerges. The adults are small. The hind-wings are pale-buff, and the fore-wings are mid-brown or greyish-brown with thin vague lines of darker brown colour along the wing veins. The males are smaller than the females.

Sexual activity usually begins shortly after adult emergence. There is a preoviposition period of about 2 days. Egg-laying mainly occurs during the night. The greatest numbers are laid on the second and third days after emergence, although oviposition may continue throughout life. Eggs take about 2-3 days to hatch. Optimum conditions for larval development of C. cephalonica are 30 – 32.5 °C and 70 per cent RH, at which, the period from egg hatch to adult emergence is only 26-27 days. There is considerable variation in the number of larval instars; however, males generally have 7 and females have 8. The last-instar larvae pupate within the food. The adults emerge through the anterior end of the cocoon, where there is a line of weakness. The sex ratio is 1:1. The adult moth is nocturnal and is most active at nightfall.

Entomopathogenic nematode (EPN) viz., Heterorhabditis, Neosteinernema and Steinernema have been recovered from many regions of the world. They selectively infect many insects and a few other arthropods but do not adversely affect mammals or plants. They are actually vectors of pathogenic bacteria, cause relatively rapid death of the host (24 – 48 h) and show considerable potential in biological control of insect pests in the integrated pest management systems.

EPNs are similar to insect parasitoids in that the immature form develops at the expense of one host individual; they are ecologically similar to both parasitoids and arthropod predators because the host individually killed. However, these nematodes differ from predators and parasitoids in two ways: (1) the nematode is mutualistically associated with a bacterial pathogen which actually kills the host, and (2) the pathogenicity of the bacterium has a great influence on the efficacy of the system (Ehler, 1990). The purpose of this chapter is to introduce EPN as biocontrol agents in this refresher course and to provide an overview of some current issues in biological control of insects with particular emphasis on their relevance to the use of EPNs.

Biological control is defined as the action of natural enemies which maintains a host population at levels lower than would occur in the absence of the enemies. Natural enemies will include parasitoids, predators, nematodes, microbial pathogens and their gene products. Entomologists generally divide biological control into two categories: (1) natural biological control which is effected by native or coevolved natural enemies in the native home (origin) of a given insect and (2) applied biological control which due to human intervention. Applied biological control is further divided into classical biological control (introduction of exotic natural enemies) and augmentative biological control (enhancement of natural enemies already in place).

Introduction

Studies on biocontrol agents have revealed the presence of a diverse range of antifungal metabolites derived from amino acid, polyketones and isoprenoid biosynthetic pathways, each of which has distinctive regulatory points and is subjected to different stimuli within organisms. In this experiment, the extraction of secondary metabolites from the culture of T. viride and Chaetomium globosum will be demonstrated.

Agarose gel electrophoresis is used for the fractionation and characterization of nucleic acids. It is a simple and highly effective method for separating, identifying and purifying DNA fragments of various sizes. DNA can be checked for size, intactness, homogeneity and purity by this technique. Agarose is a purified form of agar and forms a gel by hydrogen bonding of the agarose monomers. Increasing the Agarose concentration of a gel reduces the migration speed and enables separation of smaller DNA molecules. Shorter molecules move faster and migrate further than longer ones.

Aim: To study the size of the DNA extracted from the fungal culture.

Principle: Ethidium Bromide added to the gel interacts with the bases of DNA and fluorescence orange when irradiated with UV light.