eChapters

Abstract

The unique characteristics of mare’s milk help to prevent many chronic diseases. The health beneficial properties of this milk is even acknowledged since ancient time and this milk is being successfully used as medicated food against a wide range of chronic diseases. The merits of mare’s milk has also been noted by the cosmetic and pharmacy industries. Lastly, considering many health beneficial effects of this milk, attempt should be made to augment the production and popularization of mare’s milk. In this chapter different aspects of mare’s milk has been pointed out in different sections and subsections.

Introduction

Globally, 16.9% of milk consumed by humans comes from species other than cattle. Non-cattle milk is linked more to territories than cows’ milk: sheep in the Mediterranean basin, horse in Central Asia, yak in Himalayas, camel in desert regions. The domestic equid species (horse and donkey) belong to the taxonomic order Perissodactyla, family Equidae and genus Equus. The nutritional and therapeutic properties of horse (Equus caballus) milk have been known since ancient times according to the historian Erodoto (V century BC). Although horses are of minor importance for milk production in comparison with cows, buffalo, sheep and goats, they have been traditionally important dairy animals in Mongolia and in the southern states of the former Soviet Union, e.g., Kazakhstan, Kyrgyzstan and Tajikistan. Aristotle (322 BC) reported the existence of a dairy product in Mongolia that processed mare’s milk to transform it into a fermented product with an alcohol content of 3% and Herodotus also reported something very similar performed by the Scythians;

Marek’s disease is a herpesvirus-induced neoplastic disease of chickens characterized by infiltration of various nerve trunks and/or organs with pleomorphic lymphoid cells. Marek’s disease is world wide in distribution. Marek’s Disease has been named after Josef Marek who published the disease in 4 roosters in 1907.

Occurrence

In chickens only, however turkeys and quail have limited susceptibility. Lesions similar to MD have been seen in pheasants, ducks, pigeons, geese, canaries, budgerigars, swans and great horned owls. However, aetiology for lesions in these species has not been proved. It may appear at 3-4 weeks but usually after eight weeks, with major losses from 12-25 weeks. Morbidity and mortality varies depending on the virulence of MDV.

In India marigold is one of the most commonly grown flowers and used extensively on religious and social functions in different forms. Because of their ease in cultivation, wide adaptability to varying soil and climatic conditions, long duration of flowering and attractively coloured flowers of excellent keeping quality, the marigolds have become one of the most popular flowers in our country. Flowers are sold in the market as loose or as garlands. Due to its variable height and colour marigold is especially use for decoration and included in landscape plans.

1) Botrytis Gray Mould
Diagnostic Symptoms
• Appearance of a gray, fuzzy mold leaves, stems and on flowers of or plants. Also called, “gray mold”.
• Spotting or blight on leaf and flower stem lesions, and dieback.
• Flower parts become necrotic and die(Fig-1).
Etiology
Domain : Eukaryota
Kingdom : Fungi
Division : Ascomycota
Class : Leotiomycetes
Order : Helotiales
Family : Sclerotiniaceae
 

Bud caterpillars : Helicoverpa armigera : Noctuidae

Eggs are laid singly on young buds.  Larvae feed on developing flowers by damaging florets. They can also eat the leaves.

Leaf webber : Phycita sp. : Phycitidae

Larvae of Phycita sp. feed on heads of buds and flowers.

Marigold is one of the easiest annual flowers to cultivate, having wide adaptability. Flowers are attractive, have good keeping quality with a wide spectrum of colour, shape and size. They produce marketable flowers in a short time and are used widely for religious and social functions as well as for garden display. 

Importance and Uses

 Marigolds are sold as loose flowers, as cut flowers and widely used for various decorations and garland making. They are also excellent landscape plants widely used for bedding purpose, in herbaceous borders and can also be grown in pots. French marigolds, being dwarf, are suitable for hanging baskets, rockeries, edging and window boxes. In fact, marigolds rank next only to jasmine in popularity in South India 

Marigold (Tagetes spp.) an annual flowering plant belongs to family Asteraceae, alternately known as Compositae. The word “marigold” means “Mary’s Gold” and refers to the “Virgin Mary”. However, word “Tagetes” named after the Etruscan God “Tages” who supposedly emerged from the earth as it was being ploughed and was imbued with the power of divination. Cultivated species include Tagetes erecta commonly referred as African marigold and Tagetes patula as French marigold. Marigold commonly propagated by seed and cutting is a leading loose flower of India. Selection of variety is favoured by a higher proportion of double inflorescences.

Marigold (Tagetes spp.) an annual flowering plant belongs to family Asteraceae, alternately known as Compositae. The word “marigold” means “Mary’s Gold” and refers to the “Virgin Mary”. However, word “Tagetes” named after the Etruscan God “Tages” who supposedly emerged from the earth as it was being ploughed and was imbued with the power of divination. Cultivated species include Tagetes erecta commonly referred as African marigold and Tagetes patula as French marigold. Marigold commonly propagated by seed and cutting is a leading loose flower of India. Selection of variety is favoured by a higher proportion of double inflorescences.

ORIGIN AND DISTRIBUTION

Origin of marigold is central and South America especially Mexico (Kaplan, 1960). Bailey (1951, 1963 and 1976) reported that marigold is a native to Arizona and Mexico to Argentina.

During early 16^th century it spread to various parts of world from Mexico. African marigold (Tagetes erecta) first reached Spain and from there it become popular as rose of Indies in Southern Europe. Major work on improvement and cultural practices on marigold have been done in USA, Switzerland, France and Germany. In India, marigold was introduced by Portuguese and it became popular and spread quickly because of its wide adaptability to varying climatic and soil conditions. Marigold is well distributed in different states and union territories of India (Singh, 2006).

SPECIES

Earlier, Rydberg (1915) reported that genus Tagetes comprises about 33 species and Bailey (1951 and 1976) mentioned over 30 species, however, Turner and Nesom (1993) reported that Tagetes is made up of nearly 55 species distributed in the American continent, most of them located in Mexico. The two species Tagetes erecta and Tagetes patula are commercially important. Details of these species are given below.

INTRODUCTION

Marigold is an important flower crop and constitute as one of the five most commonly cultivated and used flower in urban and rural India. They are extensively used for making garlands, beautification and other purposes i.e. pigment and oil extraction and thera eutic uses A art from these uses marigold is a widely grown plant in gardens and pots. It is highly suitable as a bedding plant, in a herbaceous border and is also ideal for newly planted shrubberies to provide colour and fill the space. French marigold is most ideal for rockery, edging, hanging baskets and window boxes. Flowers remain fresh for 4-5 days at room temperature and are used for religious offerings and social functions. Recently marigolds are grown commercially for extraction of carotene pigments mainly xanthophylls. The carotenoid extracted from petals are added to poultry feed for intensification of yellow colour of egg yolk. Lutein which is the major constituent of xanthophylls is used for colouring food stuffs. Purified extract of marigold petals containing lutein dipalmitate is marketed as an ophthalmologic agent under the name adaptinol. Dietary carotenoids can be used to treat cancer and photosensitivity diseases.

Marigold leaves contain a distinct odoriferous oil. Wild marigold (Tagetes minuta) oil has valuable and precious essential oil which is used in high grade perfumes and cosmetics. Ketones present in the oil are base material for synthesizing aroma chemicals. Marigold essential oil has bronchodilatory, tranquilizing, anit-inflammatory as well as insect repellent activities. The floral extract of marigold is used for treating eye diseases and ulcers.

Abstract: Among the annual flowers, marigold is commercially important loose flower crop in India. Marigold types with odourless foliage with orange to light lemon colour are available in world market. Being cross pollinating and seed propagated, progeny can segregate widely for traits due to heterozygosity. Improvements in marigold as cut flowers, larger flowers, more intense flower colour, an expanded flower colour range, more uniform plant habit, earlier flowering, more xanthophylls and extended bloom time are future breeding objectives. The use of intraand interspecific hybridization, heterosis, polyploidy and genetics of traits have been presented with a focus on creating novel transformations for this crop in future.

Introduction

Marigold is an important flower crop in India. They are extensively used for making garlands, beautification and other purposes i.e. oil extraction and therapeutic uses. Apart from these uses marigold is a widely grown plant in gardens and for pots. Marigolds are also grown commercially for extraction of carotene pigments mainly xanthophylls. The carotenoid extracted from petals are added to poultry feed for intensification of yellow colour of egg yolk. Lutein which is the major constituent of xanthophylls is used for colouring food stuffs.

Marigold plantation has been found beneficial to reduce the population of nematodes. Root-knot (Meloidogyne spp.) can be effectively managed in the field without pesticides.

Genetics

Marigold belongs to family Asteraceae. There are about 33 species of the genus Tagetes. Important species are T. erecta, T. patula, T. tenuifolia, T. lucida, T. lacera, T. lemmonii, T. minuta, T. pusilla and T. corymbosa. The chromosome number in diploid species i.e. T. erecta, T. tenuifolia, T. elongata, T. lemmonii, T. jaliscensis and T. lucida is 2n = 24, however, tetrapoid species i.e. T. patula, T. minuta, T. remotifolia, T. mendocina and T. biflora is 2n = 48.

Marigold (Tagetes spp.) is an important annual flower crop; native to Central and South America especially Mexico and belongs to family Asteraceae (Compositae). It is commonly known as “Gainda” in India and also called as “Friendship Flower” in United States of America. Loose flowers of marigold are in great demand throughout the year. It is commercially grown in tropical and subtropical regions of India owing to its ease in cultivation, hardy nature, wider adaptability and year round availability of single, semi-double and double flowers of vibrant colours. Marigold is grown over an area of about 66.09 thousand hectares with production of 603.18 thousand MT loose flowers in India. Major states involved in loose flower production of marigold are Karnataka, Madhya Pradesh, Gujarat, Andhra Pradesh, West Bengal, Maharashtra, Haryana and Chhattisgarh. In India, Assam is the only state, which produces marigold for both cut as well as loose flower purpose on an area of about 0.61 thousand hectare (Anonymous, 2016a).

Marigold is a cross-pollinated flower crop and it is pollinated by insects (Kaneko et al., 1990). It bears terminal and solitary inflorescence, known as head or capitulum, which possesses long and hollow peduncle with swollen apex and campanulate involucres (Setshogo, 2005). Capitulum consists of peripheral ray florets and central disc florets. Ray florets are pistillate, ligulate, sessile, epigynous and zygomorphic, whereas, disc florets are bisexual, tubular, sessile, epigynous and actinomorphic (Subrahmanyam, 1996 & Setshogo, 2005).

African marigold (Tagetes erecta L.) is a diploid species (2n = 2x = 24), while, French marigold (Tagetes patula L.) is a tetraploid species (2n = 4x = 48) with basic chromosome no. of x = 12 as revealed by breeding work of various researchers (Towner, 1961, Chen and Lin, 1981, Chen and Lin, 1982, Serrato-Cruz et al. 2000, Zhang et al., 2011). Tagetes erecta and Tagetes tenuifolia are diploid species, whereas, Tagetes patula, Tagetes minuta, Tagetes biflora and Tagetes remotiflora species are tetraploid in nature (De, 2017). On the basis of floral morphology; three different types of male sterile systems i.e. apetaloid, petaloid and gynomonoecious types was observed in African marigold (Prakash et al., 2016). Out of these, apetalous male sterility (femina type) is more reliable and preferred over petalous male sterility because later is more prone to break down (Goldsmith, 1968 & Janakiram, 2004). Apetaloid male sterility was controlled by single recessive gene (Prakash et al., 2016). A monogenic recessive male sterile apetalous mutant was detected from T. erecta population by Khoshoo (1979). Cyto-genetic male sterility is also found in marigold (De, 2017). Petaloid and gynomonoecious male sterile systems were controlled by cytoplasmic inheritance (Prakash et al., 2016). In marigold, cytoplasmic genetic male sterile lines were also developed for commercial production of F_l sterile hybrids (Pratibha et al., 2000).

Marigold is one of the dominant loose flower crops in India, particularly important for religious ceremonies, festivals, weddings, garlands and decoration. The principal cultivated types include African marigold (Tagetes erecta) and French marigold (Tagetes patula). The chapter discusses the origin, distribution, genetics, botanical characteristics and important varieties of marigold. It also highlights emerging applications such as lutein extraction, natural colourants, culinary uses and companion planting. Detailed production practices cover climate, soil, propagation, nursery management, land preparation, nutrient management, planting, irrigation, pinching and flower harvesting. Important pests and diseases, postharvest handling and yield are also discussed for efficient commercial production.

ABSTRACT

The Mumbai region of Maharashtra has been witnessing very rapid urbanization development in terms of industrial, commercial and residential establishments. This has resulted in reclamation of tidal flats and estuarine regions. The ‘marine clay', characteristic of this environment has high swelling potential and consequently affects the performance of the structures. This will have a serious negative impact on the urbanization expansion. In this study, an attempt is made to map the spatial distribution of these expansive clays using the state of art Hyperion data. Reflectance spectra of representative samples collected from the field is used to map marine clays using Spectral Angle Mapper (SAM) technique. Laboratory analyses of these foundation soils (reflectance spectroscopy, mineralogy and Atterberg limits) indicate that they have significant amount of montmorrilonite and has good swelling potential (A_C>1.4). The derived expansive clay abundance map will be of use while planning appropriate remedial measure and future development.

Key Words : Swelling clay, Spectral characters, Geotechnical properties

1. I Kilo joule - 10 joule.

2. 1 Mega joule -10º joule.

3. 1 Kilowatt hour-3.6*10 joule.

4. The heat engine converts heat energy into mechanical energy.

5. The machine which converts one forms of energy to another form is called as engine or prime mover.

6. Fahrenheit scale is called as British thermal unit and denoted by B.Th.U.

7. Internal combustion engines using coal gas as fuel was made by Frenchman Etienne Lenoir (1860).

8. Silent gas engine was developed by Germans Nileolansotto & Eugen Langen.

9. Rudolph diesel made the first diesel engine in 1894.

10. TDC is also called as inner dead centre.

The resources on the land have started showing sign of faster depletion owing to the population explosion, over exploitation of mineral and water resources and also the improper exploitation and the related wasting of the natural resources. Hence a time has come to search for more and more newer natural resources amongst which the ocean resources gain supreme importance as this treasure is yet to be hunted due to the non - availability of suitable technology for the same.

Such ocean resources mainly include the mineralogical and biological resources. The ocean biological resources, their birth, growth, population and migration greatly depend as chlorophyll content, total dissolved oxygen, total suspended solids, salinity etc. These parameters which constitute the marine environment drastically change in a quick span of time owing to the dynamically changing marine forces.

Abstract

GIS in marine fisheries has taken off much later than its application in land. But the strides made in this field since 1990’s has been quite significant, as many serious mapping studies and modeling initiatives have been recorded. With the third dimension added to the 2D spatial data, the marine GIS have all the ingredients of making it a fertile domain for research for decades to come. Most of the studies and products pertaining to GIS in fishery management had been revolving around mapping of habitats, geo-referencing of catch and effort data and modeling linking oceanographic and biogeochemical factors with living resources. Satellite remotely sensed information like ocean colour can act as a surrogate to fish biomass. Further remotely sensed SST, wind and other parameters can be effectively modeled to predict fish abundance, their habitat and related aspects. This manuscript pours into the various products and outputs based on GIS in marine fisheries around the world and the Indian initiatives.

Key words: GIS, marine resources, oceanography, primary productivity and climate change

1.0 Introduction

Geographic Information Systems (GIS) have been one of the most prolific scientific enhancements in recent past, which by virtue of out of box expansion of traditional database concept have opened up an entirely new vista of knowledge and opportunities. It’s probably one such unique application where information collation and analysis happen hand in hand resulting in palpable mining of data in perspective. A formal introduction to GIS would define it as a collection of computer hardware, software, data and personnel designed to collect, store, update, manipulate, analyze and display geographically referenced information. By virtue of its conceptualizations, there are no single GIS; systems can be assembled in an infinite number of ways. This is one such field, which probably always seeks the frontiers of innovations in the fields of ICT and modeling.

Abstract
Kerala with its extensive coastline and rich marine resources, plays a pivotal role in India’s fisheries sector. This paper delves into the multifaceted dimensions of marine fisheries in Kerala, highlighting its economic, environmental, and social significance. Kerala boasts an exclusive economic zone (EEZ) spanning 2.19 lakh sq km and a coastline spanning 590 km. Its marine waters, especially in the Arabian Sea, are highly productive. The paper explores the recent advancements and achievements in Kerala’s fisheries sector, driven by mechanization and motorization initiatives. In 2022, Kerala recorded its highest marine fish catch of the decade, totalling 6.87 lakh tonnes, marking a 24% increase from the previous year. Key species such as oil sardine and Indian mackerel demonstrated significant recoveries, underscoring the sector’s resilience and adaptive capacity.
Sector-wise analysis reveals that pelagic finfishes dominate the landings, with oil sardine and Indian mackerel leading the pack. Demersal and crustacean landings also contribute significantly to the state’s fishery output. The abstract discusses the distribution of catch among different districts, with Ernakulam emerging as a major contributor. Furthermore, the socio-economic impact of marine fisheries is examined, highlighting its role in livelihoods for approximately 8 lakh people across 222 fishing villages. The sector not only provides income but also supports cultural identities and community cohesion along Kerala’s coastal areas.
Keywords: Marine resources, Production, Kerala, Landing and Fisheries sector.

ABSTRACT

The world ocean with a coastline of approximately 3,50,000 km is the largest ecosystem on earth. Marine organisms are the major, sustaining components of ecosystem processes and are responsible for biogeochemical reactions that drive our climate changes. Despite this, many marine organisms are poorly described and little is known of broad spatial and temporal scale trends in their abundance and distribution. With new molecular and analytical techniques we can advance our knowledge of marine biodiversity at the species level to understand how marine biodiversity supports ecosystem structure, dynamics and resilience. We can then interpret environmental, ecological and evolutionary processes controlling and structuring marine ecosystem biodiversity. In biotechnology applications marine microbes represent a potential source for numerous commercially important biomolecules and their bioremediation capabilities are also remarkable. They also play a crucial role in decomposition of organic matter and cycling of nutrients. Microbes also serve as food for some bottom-living organisms. Understanding and preserving biodiversity was one of the most important global challenges for the past 20 years and will continue to be an important scientific issue into the new millennium. Our knowledge of marine microbial diversity has, however, been severely limited by relying on microorganisms that have been cultured which are less than 1%.

Introduction
Marine Pollution
The pollution of the sea substances that humans have directly or indirectly introducedinto the marine environment, which can have negative impacts on the ecosystem, including health risks to people, obstructions to marine activities, and a decline in the quality of seawater.
It happens when invading species or chemicals or particles or garbage from industry, agriculture, or residential sources find their way into the ocean and can have detrimental impacts.
The majority of maritime pollution originates on land. Nonpoint causes of pollution include wind-blown debris and agricultural runoff.
A great deal of potentially harmful substances stick to small particles, which plankton and benthos animals (most of which are deposit or filter feeders) then consume.
Pesticides swiftly find their way into marine food webs after entering the marine ecology. These pesticides can induce diseases and mutations once they enter food webs, which can be dangerous for both humans and the entire food chain.
Marine food webs may also be exposed to toxic metals. These have the power to alter biochemistry, behavior, reproduction, tissue composition, and growth inhibition in marine organisms. Additionally, a lot of animal diets contain a lot of fish meal or fish hydrolysate. Toxins from the sea can thus spread to land animals and then show up in meat and dairy products.

Increasing pressures exerted on the environment by humans make protection of natural areas crucial for the preservation of biological diversity. Protected areas are one of the most effective tools available for conservation of biodiversity.

The IUCN defines a Protected Area as ‘an area of land and/or sea specially dedicated to the protection and maintenance of biological diversity, and of natural and associated cultured resources, and managed through legal or other effective means.

Since 1986, the IUCN commission on National Parks and Protected Areas (CNPPA) has been promoting the establishment and management of a global representative system of Marine Protected Areas (MPAs) which play a critical role in the conservation of biodiversity. MPAs are growing in importance globally as practical and potentially effective options for the management of fisheries, the protection of biodiversity and the generation of income from eco-tourism. The effective management of MPAs to ensure that they meet their declared objectives poses many challenges.

The IUCN has defined an Marine Protected Area (MPA) as ‘Any area of the intertidal or subtidal terrain, together with its overlying water and associated flora, fauna, historical and cultural features, which has been reserved by law or other effective means to protect part or all the enclosed environment (Kelleher,1999).

Abstract
The oceans, which make up more than 70% of Earth’s surface, are home to a vast variety of marine ecosystem services that sustain human civilization, health, and the economy. The 8.06% percent of the ocean covered by marine protected areas with an around of 18,200 marine protected areas with a total area about 29,202,667km2. A novel, pertinent, and science-driven framework for classifying regions and connecting their effects on people and the environment is the marine protected area system. Marine protected areas create high-quality metrics to augment the official World database on protected areas reporting by utilizing science-based frameworks to evaluate the degree of protection and stage of setup throughout the world. This article presents the most recent official coverage statistics for marine protected areas.
Keywords: Marine Protected areas, Ecosystem, Protection and International
Distribution of Marine Protected Areas
There are 8.06% marine protected areas (MPAs) in the world. The Global Ocean can be divided into areas within national jurisdiction (National Waters) and those in international waters (Areas Beyond National Jurisdiction (ABNJ)). Governments in national waters with established legislative frameworks find it easier to establish MPAs. MPA creation is more challenging in ABNJ because of the intricate legislative system that governs the state.
As a result, compared to ABNJ, a significantly larger number of MPAs have been established within national waters. 39% of the world’s ocean is made up of national waters, of which 18.43% are currently protected areas.

Introduction

The traditional approach to transferring genes from wild to cultivated species is based on interspecific hybridization followed by selection of hybrids that combine the trait with the cultivated genetic background. This breeding strategy is achieved by various backcross generations in which the selected hybrids at each generation are crossed back to the cultivated genotype with the aim of reducing the wild genome and its undesirable traits. The use of molecular markers has allowed this breeding approach to be greatly improved, since these markers directly reveal genetic variability through DNA analysis (Staub et al. 1996), and therefore their detection is not influenced by environmental effects. Since the development of numerous molecular markers for plant genometatic analysis, the possibility to select the genotype instead of the phenotype has been closely examined, leading to the concept of molecular marker-assisted selection (MAS; Paterson et al. 1991). The basic concept of MAS for crop improvement has been well discussed by Collard et al. (2005) which is most appropriate for this chapter. The most widely used markers suitable for MAS are RFLP, RAPD, AFLP, and SSR. Their common origin is point mutation or chromosome rearrangements that were accumulated during the evolution of the species without negatively influencing their survival and reproduction. The choice of the most suitable markers for MAS can differ and depends on the labor required for their detection, possibility of revealing single or multiple loci, dominant or co dominant nature and costs.

Marker Assisted Selection (MAS)

Marker Assisted Selection [MAS] is defining as the indirect selection for a desired plant phenotype based on the banding pattern of linked molecular (DNA) markers. It is based to infer the presence of a gene from the presence of a marker which is tightly linked to the gene of interest and high heritability of the gene of interest. Marker assisted selection (MAS) is also known by other names as marker aided selection and marker assisted breeding (MAB).

25.1 Introduction

The method of selection in which a gene/QTL (Quantitative Trait Locus) is selected on the basis of the genotype of the molecular marker (also called DNA marker) linked to this gene/QTL is known as marker-assisted selection (also called marker-aided selection). The idea of marker-assisted selection (MAS) was first advocated by Tanksley (1983). MAS can be used in combination with the conventional breeding methods or novel breeding schemes could be devised to take full advantage of the marker data. MAS has been widely used for introgression of the desired genes and QTLs using backcross programmes.

25.2 Marker-Assisted Backcrossing (MABC)

The backcross programme based on molecular marker is usually called marker-assisted backcrossing (MABC). MABC has three activities: (a) foreground selection, (b) background selection and (c) recombinant selection.

25.2.1 Foreground Selection

The use of molecular marker to select the gene/QTL being introgressed is known as foreground selection. The objective of foreground selection is to select the target gene, i.e., the gene being introgressed, without phenotypic evaluation for the trait governed by the target gene. Foreground selection for a gene/QTL will be highly desirable in a variety of situation, some of which are listed below:

ABSTRACT

In last twenty years, molecular biology has revolutionized conventional plant breeding techniques in many areas. The use of molecular markers in conventional breeding techniques has also improved the accuracy of selection and allowed breeders to produce varieties with combined traits that were difficult before the advent of DNA technology. Among vegetables, onion (Allium cepa L.) is the most important cash crop of India. Onion productivity in India is much lower (11.38 tones / ha.) compared to other countries (44.1 tones / ha. in Japan). Purple blotch disease (PBD) caused by Alternaria porri (Ellis) Ciferri decreases the yields by up to 50 percent. Genetically resistant onion varieties are the most economical, simple and eco-friendly mode of disease management. The advent of mo- lecular markers provided the opportunity to understand the genetic resistance more pre- cisely. In this paper we present our successful efforts in developing PCR based markers linked to purple blotch disease resistance, so that such markers can be easily integrated into PBD resistance breeding program in onion. Onion germplasm was screened for PBD resistance. Line PBR 287 was identified as a good source of resistance and the genetics of inheritance of resistance in this line was worked out. The protocols for isolating good quality DNA and PCR amplification conditions were standardized. 37 polymorphic mark- ers were identified between resistant and susceptible parents. Crosses were made between resistant and susceptible parents. Parents, F₂ and individual F₂ progenies were subjected for analysis of genetics of resistance and RAPD marker analysis. F₂ individuals segregated in 3:1 ratio for resistance. DNA from resistant and susceptible F₂ individuals was pooled separately for Bulked Segregant Analysis (BSA). BSA identified one RAPD marker of 900bp being linked to resistance. Further Chi-square test for detecting the deviation of segregation of this 900bp marker confirmed segregation with classical Mendalian ratio 3:1 for a monogenic trait. Identified marker is of great practical utility to the plant breeders to screen large number of populations for purple blotch disease resistance at the early stages of the crop even in absence of the pathogen.

ABSTRACT

During the efficient genetic transformation of plants with the gene of interest, some selectable marker genes are also used in order to identify the transgenic plant cells or tissues. Antibiotic and herbicide selective agents and their resistance gene are commonly used to introduce economically valuable genes into crop plants. According to the biosafety authority and consumer view points, the presence of selectable marker genes in released transgenic crops may be transferred to weeds or pathogenic microorganism in the gastrointestinal tract or soil, and will make them resistant to treatment with herbicides or antibiotics, respectively. Sexual crossing also raises the problem of transgene expression because redundancy of transgenes in the genome may trigger homology-dependent gene silencing. The future potential of transgenic technologies for crop improvement depends greatly on our abilities to engineer stable expression of multiple transgenic traits in a predictable fashion and to prevent the transfer of undesirable transgenic material to non-transgenic crops and related species. Therefore the development of efficient marker free transgenic plants system is very essential. That is why many technologies and various approaches designed to timely elimination of transgenes when their function is no longer needed. Due to the limited number of available selectable marker genes, in future the stacking of transgenes will be increasingly desirable. The production of marker-free transgenic plants is now a critical requisite for their commercial deployment and also for engineering multiple and complex trait. Here we describe the current technologies to eliminate the selectable marker genes (SMG) in order to develop marker-free transgenic plants and also discuss the regulation and biosafety concern of genetically modified (GM) crops.

Introduction

The main goal of plant breeding is to increase crop yield and the second goal is to improve quality, develop light- and heat-insensitive varieties, tolerance to biotic and abiotic stresses, simultaneous maturation, and water and nutrient use. Efficiency, elimination of toxins, and cultivation of different product groups allow to achieve high agricultural yields and sustainable development. Better understanding and development of genetics has increased the efficiency of plant breeding to achieve desired goals in agricultural crops. Effective and efficient use of molecular markers in crop improvement programs increases selection efficiency and productivity and accelerates the growth cycle to create new varieties with good results. Marker assisted selection (MAS) is a technique that uses markers (morphological, biochemical, or DNA/RNA variation-based markers) to indirectly select for traits of interest (e.g., productivity, disease, genetic determinants resistance, abiotic stress tolerance and/or quality). This process is used in plant and animal breeding. The rapid development of biotechnology has allowed breeders to develop better selections to replace traditional selections based on phenotypic pedigree.

1. Markers: Easily identifiable features are called markers or marker features.

2. Types of marker

These markers are three types

2.1.Morphological markers: These relate to the size, shape, color and surface area of different parts of the plant.

2.2.Biochemical markers: Such markers refer to changes in protein structure. For example, isozymes and storage proteins.

Introduction

Market access and supply chain innovation are two critical pillars that underpin the success and sustainability of agricultural produce in today's interconnected world. As the global population continues to grow, along with increasing consumer demands and evolving market dynamics, ensuring efficient access to markets and implementing innovative supply chain practices have become burning imperatives for agricultural producers worldwide.

The word market is derived from the latin word “marcatus” which means “merchandise or trade or a place where business is conducted “. The term market has been widely used in various means, such as:

• A place or a building where commodities are bought and sold (e.g. Super market)

1. ____________ aims at elimination of unhealthy and unscrupulous practices, reducing the marketing charges and providing facilities to producer and sellers in the market

a) Regional market

b) Controlled market

c) Regulated market

d) Revised market

2. The market charges are paid by the _____ of agricultural commodities in a regulated market.

a) Buyers

b) Traders

c) Farmers

d) Sellers

3. __________ is a voluntary business organization established by its members to market the farm products jointly to reap the direct benefit out of it.

a) Regulated market

Introduction

India now emerged as the world’s second largest producer of fruits, vegetables and tea holding a share of 10 percent. The production base of horticultural crops has been expending since independence (Chadha, 2009). But as a controversy to the previous statement there is a clear indication about the declining farm sector performance-in addition to its deleterious economic ramifications often causing large scale rural distress which often ends up in farmer suicides (Sundaresan, 2009). This distress conveys much on the economic rationale ruling the agri and rural domain for its incompatibility in the changing market environment. The identified major problems faced by farmers in the present scenario were non-availability of information about marketing services including availability of crop specific markets, existing demand of a commodity and daily market price for their produce which can be broadly categorized under lack of market-led extension services.

Market information

As defined by Philip Kotler, Marketing information system is continuing & interacting structure of people, equipment & procedure designed to gather, sort, analyze, evaluate, distribute pertinent, timely and accurate information for use by marketing decision makers, to improve their marketing planning, execution and control.

It is the job of collecting, interpreting and disseminating large variety of data which are needed for the smooth operation of marketing processes. In many countries Government agencies undertake this function and sometimes organisations such as chambers of commerce, board of traders etc. perform this function. The Directorate of Economics and Statistics and concerned state marketing departments collect data regarding a) quantity of various products produced in different areas, b) quantities of products held in storage, c) arrivals in various markets, d) prices prevailing in local, regional, national and international markets. The informative data so collected is disseminated through radio, television, news papers, weekly and monthly bulletins. Daily prices are published in most of the news papers. The informative marketing data is also collected through sales executives and from those personnel who are specially appointed as specialists by various trading firms.

7.1. Marketing institutions

Business organizations which have come up to operate the marketing machinery.

Individuals, corporate, co-operative and government institutions are operating in agricultural marketing.

Introduction

A fundamental issue when analysing trade policy reform in global seafood market is the extent to which domestic agricultural commodity markets in developing countries respond to changes in international prices. Price trans mission from the world to domestic markets is central in understanding the extent of the integration of economic agents into the market process.

Studies on the transmission of price signals are founded on concepts related to competitive pricing behavior. In spatial terms, the classical paradigm of the Law of One Price, as well as the predictions on market integration provided by the standard spatial price determination models (Enke, 1951; Samuelson, 1952; Takayama and Judge, 1972) postulate that price transmission is complete with equilibrium prices of a commodity sold on competitive foreign and domestic markets differing only by transfer costs, when converted to a common currency. These models predict that changes in supply and demand conditions in one market will affect trade and therefore prices in other markets as equilibrium is restored through spatial arbitrage.

The absence of market integration, or of complete pass-through of price changes from one market to another has important implications for economic welfare. Incomplete price transmission arising either due to trade and other policies, or due to transaction costs such as poor transport and communication infrastructure, results in a reduction in the price information available to economic agents and consequently may lead to decisions that contribute to inefficient outcomes. Fisheries and seafood food trade policy reform, especially, is a priority issue in the next WTO negotiations, as trade liberalization is viewed as encouraging allocative efficiency and long run growth.

1. The expansion of firms by consolidating supplementary marketing functions and activities under a single management

a) Market Segment

b) Market integration

c) Market channel

d) Market Regulation

2. When a firm gains control over other firms which performing similar marketing functions are __________

a) Vertical co-ordination

b) Horizontal integration

c) Vertical integration

d) conglomeration

3. When a firm performs more than one activity in the order or sequence of the marketing process is known as _________

a) Vertical co-ordination

b) Conglomeration

c) Horizontal integration

d) Vertical integration

4.1. Definition

Kohls and Uhl refer to expansion of firms by consolidating additional marketing functions and activities under a single management.

Establishment of wholesaling facilities by food retailers and setting up of another plant by a milk processor.

The present study examines the role of market intelligence in enhancing the efficiency and profitability of India’s agricultural sector. With agriculture contributing about 16-18% of India’s GDP and supporting 58% of the population, optimizing agricultural supply chains is crucial. Through tools like real-time data, AI, and blockchain, market intelligence enables farmers to reduce post-harvest losses estimated at INR 92,000 crores ($12 billion) annually and make informed decisions on crop cycles, pricing, and risk management. Initiatives such as e-NAM and Digital India have expanded access to data, empowering smallholders, who make up 85% of India’s farmers, with valuable market insights. The study presents case studies of successful applications, such as blockchain for organic certification in Sikkim and precision agriculture in Punjab, resulting in 20-30% income growth and 15% higher yields, respectively. Despite challenges like limited rural internet access and high costs, public-private partnerships and increasing smartphone penetration are advancing market intelligence adoption. The findings suggest that as these technologies evolve, market intelligence will be instrumental in driving sustainable growth, mitigating risks from climate variability, and securing India’s food systems for the future.

Abstract
The present study examines the role of market intelligence in enhancing the efficiency and profitability of India’s agricultural sector. With agriculture contributing about 16-18% of India’s GDP and supporting 58% of the population, optimizing agricultural supply chains is crucial. Through tools like real-time data, AI, and blockchain, market intelligence enables farmers to reduce post-harvest losses estimated at INR 92,000 crores ($12 billion) annually and make informed decisions on crop cycles, pricing, and risk management. Initiatives such as e-NAM and Digital India have expanded access to data, empowering smallholders, who make up 85% of India’s farmers, with valuable market insights. The study presents case studies of successful applications, such as blockchain for organic certification in Sikkim and precision agriculture in Punjab, resulting in 20-30% income growth and 15% higher yields, respectively. Despite challenges like limited rural internet access and high costs, public-private partnerships and increasing smartphone penetration are advancing market intelligence adoption. The findings suggest that as these technologies evolve, market intelligence will be instrumental in driving sustainable growth, mitigating risks from climate variability, and securing India’s food systems for the future.
Keywords: Market intelligence, Agricultural supply chain, post-harvest losses, Sustainable agriculture

Introduction

Focus of extension agencies on production technologies yielded very good results andhence India become self reliant on food production. Significantly,the extension systemhadplayedits role untiringlyintransfer ofproductiontechnologies from lab to land, besides the agricultural scientists, farmers and marketing network. But the farmers at individual level are not realizing remunerative prices for their produce. Add to this, the Indian farmers have to face challenges of global force that are affecting or are likely to affect Indian agriculture. This assumes greater significance in the light of the new international trading regime under WTA and the export opportunities being opened up. 

Introduction

As India was struggling to feed her population through “ship-to-mouth” strategy, the agricultural scientists focused on the enhancing the food grain production of India so that the country became self-sufficient. Through tireless efforts our country ushered into Green Revolution which marked the beginning of the era of self-sufficiency for India. The efforts of researchers were seamlessly taken to fields and communicated to masses by the efficient extension professionals of our country. The focus of extension was “Production Led”. But as time passed, India became self-sufficient in major food crops, the farmers started facing problems of marketing their surplus produce. Market gluts, non-remunerative prices, distress sales and emergence of exploitative middle men made farmers all the more helpless. It was at this time when the concept of “Production Led Extension” was thought of being reformed to “Market Led Extension” (MLE). As of today, MLE has helped many farmers market their produce easily and fetch remunerative prices of the produce. Agricultural, livestock, fisheries and all allied sectors have received benefit from this approach of extension.

Indian agriculture – the back bone of our economy accounts for about 65 per cent of the country’s employment and 20 per cent the total export earnings. Despite a drastic increase in the food grains production during the last five decades, the profit margins of farmers particularly small and medium farmers has declined due to certain serious problems like declining water table, deterioration in soil health, outbreaks of new pests diseases and weeds, and environmental pollution etc. With the globalization of market, the farmers will have to change themselves from mere producers’ in the domestic markets to producer cum seller in a wider market sense in order to best realize the returns for his investments, risks and efforts. Now the focus should be on high quality produce, low cost of production and high productivity.

When it comes to the fow of goods and services from the point of production till they reach the hands of fnal consumers, marketing is defned as “the performance of all business operations involved in that fow.”

Market structure is defned as “those aspects of the market’s organisation that appear to strategically infuence the nature of competition and pricing inside the market.”The market structure is decided by the following factors in general.

1. Number and size of the frms.
2. Degree of product differentiation.
3. Conditions for entry and exit.
4. Flow of market information.
5. Degree of integration.

Introduction
Market milk holds a crucial place in human nutrition, economic development, and the dairy industry. Its consumption patterns and marketing strategies continue to adapt, influenced by evolving lifestyles, consumer preferences, and advancements in processing and packaging technologies. Regulatory frameworks ensure its safety and quality, while the wide range of milk types caters to diverse dietary and cultural requirements around the world.
Modern dairy farms rely on advanced milking machines that prioritize both efficiency and hygiene. These machines use vacuum-operated devices to gently extract milk from cows’ teats without causing discomfort. The collected milk is immediately transferred into refrigerated tanks to maintain its freshness and prevent bacterial growth. Speed and efficiency in transporting milk to processing facilities are critical to ensuring its safety and quality.
At the processing plant, milk undergoes standardization, an essential step where the fat content is adjusted to produce different types of milk, such as whole milk, toned milk, double-toned milk, and skimmed milk. This ensures uniformity in fat levels, meeting market specifications and regulatory standards such as prescribed by FSSAI in case of India.
The milk then undergoes pasteurization, a process of heating it to a specific temperature for a defined period to eliminate harmful bacteria while preserving its nutritional value. Pasteurization has significantly improved milk safety, reduced health risks, and extended its shelf life. Following this, the milk is homogenized, where fat molecules are evenly distributed to prevent cream separation. This centres a smooth texture and consistent flavor preferred by consumers.

Abstract

Entrepreneurs take responsibility of their ‘business’ tad differently than their counterpart, the traditional businessman. A businessman walks on the pre-defined route whereas the entrepreneur creates his own alley-way, which if successful, becomes the rulebook for that trade. Lately, ecological issues have taken centre-stage as climatic changes have endangered the sustainable growth of the business. A new breed of entrepreneurs, alias ecopreneurs, functions in this ‘new’ space and endeavour to frame an innovative business landscape. Ecopreneurs possess singular focus on the safe-keep of the ecology. But in order to sustain the efforts of businesses in ecology, it is imperative that they remain economically viable too. Thus, ecopreneurial process is not only about fulfilling the business’ responsibility towards ecology, but also about the marketability of the product or service. In order to evaluate the ecopreneurial process, organic farming is considered as a case. In this paper, selected organic farms are analysed in an environment - market continuum, developed particularly for this study which is in tandem with the works of Schaltegger (2002) and Blake and Mouton (1964).The study reveals inadequacy of market performance of the individual and farm level ecopreneurs in the state of West Bengal, in spite of their strong inclination towards care for the environment.

A concentrated, hydrophobic liquid containing volatile aroma compounds obtained from aromatic plants is referred to as essential oil. It is so-called to indicate that the oil is the fragrant essence of the plant from which it is extracted generally through various distillation methods. The economics working behind essential oils has three components. First, the cultivation of herbs and plants used to extract essential oils; second, the actual process of extraction of the oils; and, third, the marketing and actual sale of these oils. But the major emphasis is often given to the agrotechnology, since the crop yield and yield of the essential oil depends on the quality of cultivation. The cost of cultivation of herbs in the developing countries per hectare is estimated at US $ 600 to 1100. This cost mainly comprises labour cost and as such this industry is uniquely suitable only for developing nations. The revenue from essential oils after the extraction per hectare is estimated to be around US $ 2500 to US $ 6500. According to industry sources, more than 3,500 essential oils are known. Out of these, some 400 are used for commercial purposes on a regular basis. These are obtained mostly from agricultural plants, some from wild plants that may be seasonal or perennial. Oil can be extracted from any part of plants—from leaves, flowers, fruits, roots etc. Whatever the source of essential oils, they have complex compositions, containing alcohol, aldehydes, ketones, phenols, esters, ethers and turpenes in varying proportions.

2.1. Market structure

Refers to those organizational characteristics of a market which influence nature of competition and pricing, and affect the conduct of business firms.

Those characteristics of market which affect the traders behavior and their performances.

Formal organization of functional activity of a marketing institution.

A knowledge of structure is essential to identify imperfections in performance of a market.

Market survey and analysis of different types of home appliances available in the market.