eChapters

India is basically an agrarian economy where 57.8% of rural households are engaged in agriculture (Situation Assessment Survey of Agricultural Household, 2021). 48.9 per cent of the Indian work force is employed in the agricultural sector, (ILO, 2016). The estimated Indian population for the year 2050 is 1.7 billion which will raise our food demand to ∼400 million tonnes (Rao et al., 2018). In this context, Indian agricultural production system will have to take up the challenge to feed l7.5% of the global population with only 2.4% of land and 4% of the available water resources at its disposal.

Introduction

A geographical information system (GIS) is a kind of database that has software tools for handling, analyzing, and presenting spatial data and descriptions of phenomena that have a location component. This system can also involve human users and support staff, procedures and workflows, a body of knowledge about relevant concepts and methods, and institutional organizations in a wider sense. GIS technology is a key part of spatial data infrastructure, which the White House describes as “the policies, technologies, standards, human resources, and related activities that are necessary to acquire, process, distribute, use, maintain, and preserve spatial data.”

• General considerations

 • Polyacrylamide gel electrophoresis (PAGE)

 • SDS-polyacrylamide gel electrophoresis (SDS PAGE)

• Nucleic acid hybridization

 • Radio labelled probes

 • Biotin labelled probes

 • DNA finger printing

 • Polymerase chain reaction (PCR)

 • Reverse transcriptase polymerase chain reaction (RT PCR)

• General considerations

 • Indications

 • Limitations

 • Techniques of bone marrow examination

 • Cell types

 • Cellular composition of bone marrow

 • Morphology of the cells

 • Interpretation of bone marrow examination

• General considerations

 • Blood glucose

• Total serum protein

 • Serum albumin

 • Serum globulin

 • Serum gamma globulin

 • Serum calcium

 • Serum inorganic phosphorus

 • Serum magnesium

 • Serum cholesterol

 • Serum creatinine

 • Serum urea

 • Serum chloride

 • Serum sodium

 • Serum potassium

 • Serum Glutamic Oxaloacetic Transaminase

 • Serum Glutamic Pyruvic Transaminase

 • Serum alkaline phosphatase

• General considerations

 • Collection of blood

 • Storage

 • Examination of blood

 • Coagulation time

 • Bleeding time

 • Determination of movement of parasites under wet film

• Total erythrocyte count

 • Total leucocyte count

 • Differential leucocyte count

 • Hemoglobin

 • Erythrocyte sedimentation rate

• Hematocrit value

 • Mean corpuscular volume

 • Mean corpuscular hemoglobin

 • Mean corpuscular hemoglobin concentration

 • Erythrocyte fragility test

• Epidemiological definitions

 • List of tests for international trade

 • OIE listed diseases

 • Solutions and reagents

 • Age and body weight of animals

 • Rectal temperature of animals

 • Normal values of blood vascular system

 • Vaccination schedule

• Nutritional diseases

 • Viral diseases

 • Prion, Mycoplasmal, Rickettsial, Chlamydial, Spirochaetal and Bacterial

diseases

 • Fungal diseases

 • Parasitic diseases

 • Toxic conditions

 • Miscellaneous conditions

• General considerations

 • Purpose

 • Scope

 • Five pillars of GLP

 • Good laboratory practice principles

• Test facility organization and personnel

 • Quality assurance programme

 • Facilities

 • Apparatus, materials and reagents

 • Test systems

 • Test and reference items

 • Standard operating procedures

 • Performance of the study

• General considerations

 • Purpose and goals of laboratory

 • Standards, guides and references

 • Accreditation for competence

 • Identification of scope

 • Test methods

 • Benefits of ISO17025 accreditation

 • Processing of application

• General considerations

 • Quality management principles

 • Objectives of 9001:2008

 1. Scope

 2. Normative reference

 3. Terms and definitions

 • Main clauses and sections of ISO 9001:2008

 4. Quality management system

 5. Management responsibility

 6. Resource management

 7. Product realization

 8. Measurement, analysis and Improvement

• General considerations

 • Biosafety levels-1 and -2

 • Biosafety level-3

 • Biosafety level-4

General Considerations

Biosafety is a combination of laboratory practices, techniques, equipments and other facilities appropriate for the protection of hazards posed by infectious agents handled in the laboratory. There are guidelines for maintaining the biosafety in laboratory at various levels described as under:

• General considerations

 • Ethics for veterinary diagnostic laboratory personnel

 • Safety measures in the laboratory

 • Accidents

 • First aid

 • Acid burns

 • Alkali burns

 • Poisoning

 • Burns caused by heat

 • Injuries caused by broken glass

 • Contamination by infectious materials

 • Bodily damage by electric shock

 • Precautionary measures

• General considerations

 • Steps in validation

 • Step1- Feasibility studies

 • Step 2- Assay development and standardization

 • Step 3- Determining assay performance characteristics

 • Step 4- Monitoring validity of assay performance

 • Step 5- Maintenance and enhancement of validation criteria

 • Measurement of uncertainity of a diagnostic test

• General considerations

 • Tuberculosis

 • Johne’s disease

 • Brucellosis

 • Infectious bovine rhinotracheitis

 • Campylobacteriosis

 • Tichomonosis

• General considerations

 • First hand information and constitution of a team

 • Gathering the disease information from records and the owner

 • Animal data

 • Previous illness

 • Present disease

 • Proforma for animal examination report

 • Observation of team

• Logical clinical examination of animals

 • Disease investigation kit

• General considerations

 • Cyanide poisoning

 • Nitrate poisoning

 • Nitrite poisoning

 • Oxalate poisoning

 • Strychnine poisoning

 • Arsenic poisoning

 • Lead poisoning

 • Thalium poisoning

 • Ergot poisoning

 • Carbon monoxide poisoning

 • Phenothiazine poisoning

 • Pesticide poisoning

• Allergic Tests

 • Single intradermal test

 • Tuberculin test

 • Johnin test

 • Double intradermal test

 • Comparative test

• Mallein test

 • Immunofluorescence Techniques

 • Direct method

• Indirect method

 • Immunoperoxidase Techniques

 • Detection of antigen

• General considerations

 • Agglutination

 • Slide agglutination test

 • Tube agglutination test

 • Capillary tube agglutination test

 • Plate agglutination test

 • Hemagglutination test

 • Hemagglutination-inhibition test

 • Coagglutination test

• General considerations

 • Scope

 • Microscopic examination techniques

 • Smear

 • Vital staining

• Routine histopathological techniques of sectioning

 • Collection of tissues

 • Fixation

 • Washing

 • Dehydration

 • Clearing

 • Impregnation

 • Casting of blocks

 • Trimming

 • Section cutting