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Site of incision: Abduct the hind limb well apart and remove the skin covering the medial aspect of thigh. To obtain this make an incision longitudinally along the entire length of pelvic symphysis. From both ends of this incision make vertical incision up to medial aspect of stifle joint and joins distal end of these vertical incision together and remove the skin to expose the muscles of medial aspect of thigh region. To expose deeper muscles incise the vertebral attachment of biceps femoris, reflect it caudally by incising muscle close to stifle than reflect middle gluteus muscle dorsocranially and observe the deeper layer of muscles.

Site of incision: Make an incision from distal end of cranial margin of stifle joint to coxal tuber and extend it dorsally up to mid dorsal line of the body. Make another incision at mid caudal of thigh from stifle region to tuber ischii and extend it to mid dorsal up to the base of the tail and then connects the distal ends of both previous incisions and reflect the skin up to mid dorsal line of the body.

Site of incision: Make a transverse incision below the anus between lateral margins of tuber ischia. Then from each end of this incision make an oblique incision caudo-cranially so that these join midventrally each other between the thighs. Remove the triangular flap of skin to expose the region.

Site of incision: Remove the abdominal viscera to expose the sublumber region.

Site of incision: Make a dorsoventral incision along caudal border of last rib up to mid ventral region of abdomen and another incision along the coxal tuber up to mid ventral region of abdomen. Joins the above two incision mid ventrally and reflect the skin up to mid dorsal aspect of lumber region.

Site of incision: Cut the skin over the back and loin region by making cranio caudal straight incision along the proximal part of 1st rib and the lateral margin of transverse process of lumbar vertebrae up to wing of sacrum and reflect the skin to mid dorsal aspect of back and loin.

Site of incision: Make a midventral incision on the sternum from cranial to caudal than from both ends of this incision make cranial incisions along the caudal border of shoulder and arm region to mid dorsal aspect of back and along last rib to mid dorsal aspect of back, respectively. Reflect the skin flap from ventral aspect to mid dorsal region of back.

Site of Incision

Make an incision around the eyeball at the margin of orbit.Then remove the supraorbital process by cutting dorsal and ventral end to access inside the orbit. Remove all the attachments of eyeball from osseous orbit through carefull dissection. Then remove all the extraoccular and periorbital fat to expose the muscles of eyeball.

Site of incision

Remove the skin of intermandibular space.Then remove one of the rami of mandible by disarticulating temporo-mandibular joint at caudal end and cut the ramus just behind the mental foramen.

Site of incision-Make a rostro-caudal incision along the ventral border of horizontal ramus of mandible then extend cranio-dorsally along caudal margin of nostrils up to middorsal line of face. From the caudal end of 1st incision make another incision dorsally up to base of the ear then passes cranially along the ventral aspect of eye up to mid dorsal line of face. Reflect the skin carefully from ventral to dorsal, carefully remove the muscle zygomaticus, malaris and nasolabialis as these are firmly attached to skin.

Site of incision- Make a mid-lateral and mid-medial longitudinal incision on forelimb from elbow joint to 3rd digit. Join the above incisions transversally on caudal aspect at elbow joint above and 3rd phalanx below, respectively. Remove the skin on palmero-medial aspect of limb.

Site of incision

Make a mid-lateral and mid-medial longitudinal incision on forelimb from elbow joint to 3rd digit. Joins the above incisions transversally on cranial aspect of elbow joint and 3rd phalanx, respectively. Remove the skin on dorsolateral aspect of limb

Site of incision: Detach the limb from the trunk by cutting various muscles viz; pectoral muscles, serratus ventralis, latissimus dorsi, brachiocephalicus, omotransversarius, trapezius, rhomboideus and long head of triceps.

Site of incision

Remove the skin flap of pectoral region to expose the pectoral muscles.

Site of incision: Make a straight dorso-ventral incision through skin from the summit of dorsal vertebral spine to the cranioventral aspect of shoulder joint. Extend it up to the cranial margin of shoulder joint, then caudo-ventrally up to caudal most part of elbow joint. Make another ventrodorsal straight incision from caudal most point of elbow joint to the summit of vertebral spine then reflect the skin flap from ventral to dorsal up to mid dorsal line of the body. Remove the facia to expose the various muscles of this region.

Myology: It is the branch of biological science which deals with the description of different muscles and their accessory structures.

Muscle is a contractile tissue present in all animals. Its function is to produce motion. Impulses from nerve cells control the contraction of each muscle fiber. Contractile part of muscles is muscular tissue.

There are three kinds of muscular tissue in the body:

a. Striated or striped muscle

b. Non-striated or unstriped or smooth muscle

c. Cardiac muscle.

For dissection purpose only striated muscles are included in this book.

Sex determination is one of the first aspects of nematode biology to be systematically characterized with genetic analysis and the depth of this analysis has made it a major topic in developmental biology. It is both fair and interesting to ask how general the Caenorhabditis elegans model is likely to be, both for other nematodes as well as for animals in general. As nematodes employ many different reproductive strategies, often related to parasitic life histories, one might expect similarly variable sex determination mechanisms. However, comparative studies of sex determination based on the C? elgans model have revealed both rapidly evolving and surprisingly well conserved features (Haag, 2005). This mixture of old and new (or slow and fast), along with its obvious relevance to evolution, ecology, and applied fields like agricultural and medical parasitology, has made the evolution of nematode sex determination an increasingly active research area.

Omics technologies based on genes and proteins involved in the nematode activities and the plant resistance responses are key factors to novel nematode management. Techniques such as next-generation sequencing of genomes and transcriptomes, RNAi, PIs, MAS, chemo-disruptive peptides and genetic transformation systems with reproducible results must be available within phytonematodes management strategies to enhance crop yields (Abd-Elgawad, 2022). Linking long-read sequencing to the use of high density genetic mapping can also support the detection and characterization of nematode-virulent genes. Progress in computational biology, bioinformatics, and analyzing the omics large-scale data can efficiently boost these techniques to offer accurate recognition of components and pathways engaged in nematode parasitism and plant response. Updated genome-editing devices will serve classical plant breeding and precisely translate how gene actions are linked to phenotypic performances. Yet, these methods must be cautiously used to avoid unwanted effects such as nematode virulence and pleiotropic impacts on qualitative and quantitative crop yields. Definite issues such as those related to the transfer of multiple disease resistance traits, gene original construct, and specificity of the virulent nematodes to the R-gene may arise in particular cases.

Modeling phytonematodes is helpful in the investigation of several host plant interactions and may yield useful information to be exploited in the integration of management strategies and biological control. Modeling the phytonematodes density changes in a soil/root microcosm may allow the identification of main factors active in such systems (Ciancio et?al?, 2022). The multiple tri-trophic interactions linking roots, phytonematodes and antagonists, however, originate a very complex system. It requires knowledge about the many parameters describing the nematode life stages, the interactions with the antagonistic organisms present in the microcosm, as well as their capabilities for effective and durable host regulation. Descriptive variables should also account for the effects of roots and of other environmental factors at work in the tri-trophic interaction system. Models may provide benefits, including the possibility of identifying the best moment for the inoculation of a biocontrol agent, the optimal amount of its inoculum applied to maximize its biocontrol efficacy, as well as the possibility to simulate and thus investigate its behavior once introduced in soil.

Genetic resistance is the more efficient and the more environmentally friendly way to protect crops against phytonematodes. Nematode management is complex, hence, the prevention of an initial phytonematode infestation in pathogen-free areas is of fundamental importance. However, after the entry of the nematode into an area, it is virtually impossible to eradicate because it is a soil-dwelling organism. In this regard, the genetic resistance of plants to nematodes is considered one of the most efficient and economically feasible methods to prevent production losses (Matsuo et?al?, 2012). Thus, breeding for nematode resistance is essential in crop management to obtain a high yield and stable production.