eChapters

Introduction

The pH (hydrogen ion concentration) of the environment provides the maximum effect on the growth of bacteria. Each organism grows at its optimal pH. Due to variation in other factors such as temperature, osmotic pressure etc marked changes in pH have also been observed.

Requirements

1. Erlenmeyer flasks (100 ml)
2. Culture of E. coli
3. Pipettes
4. Spectrophotometer
5. pH meter

Procedure

Abstract

In the present study, effect of phloroglucinol on the rooting behaviour of three Pyrus rootstocks viz. wild pear [P. pyrifolia (Burm F.) Nakai], kainth (P. pashia Buch. Ham.), shiara (P. serotina  Rehd.) and two scion cultivars viz. Patharnakh (P. pyrifolia (Burm F.) Nakai) and Punjab Beauty (P. pyrifolia x P. communis?) was studied. Supplementation of rooting medium (solid or liquid) with phloroglucinol (PG) had a stimulatory effect on the rooting response of wild pear, but on liquid medium the plants were vitrified with very small roots.  However, in solid medium fortified with NAA + IBA (0.25 mg l-1 each) and PG (160 mg l-1), 86.29 per cent rooting was obtained.  In kainth, Punjab Beauty and shiara supplementation of solid medium with PG had inhibitory effect on root induction.  In liquid medium, PG stimulated root initiation in kainth at 40 and 80 mg l-1, but at 160 mg l-1, it was ineffective.  In Punjab Beauty and shaira, liquid medium fortified with 40 mg l-1 PG was ineffective, whereas, at higher levels (80 and 160 mg l-1), it inhibited root induction.  Patharnakh failed to root at any of the PG level. One week culture of kainth microshoots on ½ MS solid medium supplemented with IBA (0.5 mg l-1) and PG (160 mg l-1) in dark followed by transfer to ½ MS basal liquid medium for three weeks in light resulted in 76 per cent rooting.  While, 58.70 per cent rooting was obtained in Patharnakh with similar treatment, except when IBA (0.5 mg l-1) was replaced by NAA (0.5 mg l-1).

Introduction
India with a population of 1.30 billion people is highly focusing on “Development”i.e. good food, better health & living conditions for everyone. With the increase in the incomes, people can now afford better nutrition in the form of poultry egg and meat, now poultry industry has transformed from a mere backyard activity into a major commercial activity in just four decades. India is now the world’s 3rd largest egg producer and the 5thlargest producer of broilers. The Indian poultry market witnessed an increasing trend over the past five years, growing at a Compound Annual Growth Rate (CAGR) of 8.6% (DAHD, 2023). Poultry sector in India is valued at about Rs. 1,50,000 crore (2022-23) broadly divided into two sub-sectors – one with a highly organized commercial sector with about 80% of the total market share (say, Rs. 1,20,000 crore) and the other being unorganized with about 20% of the total market share of Rs. 30,000 Crore. (DAHD, 2023). Poultry production in India has become a profitable and most popular income generating sector for the educated unemployed youth. Most of the poultry farmers are interested in broiler production due to its quick returns, less space requirement and higher weight gains. The productive potential of poultry in India has not been fully exploited due to deficit feed resources and unutilization of available improved technologies for getting high productivity from the poultry at economical rate. Hence, it is essential to further enhance the feeding value of available feed resources so as to improve the efficiency of feed utilization and minimize the cost of feed per kilogram live weight gain. The use of antibioticas growth promoters in poultry has been banned due to concern about their residues in tissue and induction of bacterial resistance. Due to these concerns, recently many feed additives has been investigated for alternatives tofeed antibiotics. The international feed industry is facing the challenge of the awareness among the consumers of meat on the risk of bringing about antibiotic resistance in pathogenic microbiota through antibiotics used in animal and poultry feeds. It has directed them towards the non-antibiotic feed additives. Among them, the feed additives of plant origin, called as Phyto-additives or Phytogenic Feed Additives (PFA) or Phytobiotics are considered to be a better alternative as non-antibiotic growth promoters, even though there are well established non antibiotic growth promoters such as organic acids and probiotics. The Phytogenic feed additives also vary widely in their botanical origin, processing and composition. They have been used in solid, dried and ground forms or as extracts or essential oils.It is reported that aromatic plants may increase feed intake and may improve secretion of endogenous digestive enzymes. It has been shown thatthe dietary incorporation of herbs may provide beneficial effect on poultry performance and health due to the antimicrobial activity of their phytochemical components. Phytobiotics have gained increasing interest as natural growth promoting feed additives in broiler production in recent years. These have wide range of medicinal properties with no residual side effects and are best alternatives to antibiotic growth promoters (Chaudhary et al., 2018, 2019, Rahman et al., 2014). Beneficial effects of these substances in poultry nutrition are due to their high content of pharmacologically active compounds stimulating appetite and feed intake, improving endogenous digestive secretion and activating immune responses (Nouzarian et al., 2011 and Toghyani et al., 2010).

Toxic elements like Cadmium (Cd), Fluoride (F), heavy metals, radioactive metals are associated with mainly rock phosphates, other organic wastes like sewage sludge and pollutants. Their chemistry is similar to plant nutrients and replace them in their physiology leading to reduced uptake of nutrients, productivity and contaminated food chain.

Seed is the primary and essential starting point of a wide range of crops, including the majority of vegetables except those vegetative propagated (e.g- potato and sweet potato). Seedling emergence and field stand establishment is one of the problems facing the growers, especially in early planting where adverse conditions are prevailed (low temperature and high soil moisture). Seed germination and seedling emergence are affected by many factors such as: seed genotype, seed quality (seed viability and seed vigor) and the environmental conditions (moisture and temperature) prevailing during seed germination and subsequent seedling emergence, assuming that the seed is not dormant.

Introduction In latest years, there has been an upsurge in customer situation over using artificial colorants in foods, especially on the subject of healthiness and safety issues (Amchova, Kotolova, & Ruda-Kucerova, 2015). Dietary colorants have been banned or regulated by the European Food Safety Authority (EFSA) and various other national authorities throughout the world because to their confirmed or suspected link to enhanced cancer progression or adverse reactions. For the same motives, various pigments are being investigated and are most effective provisionally authorized at this time. Consequently, the use of natural additives in food production is becoming increasingly popular among food companies (Schwartz, Joachim, & Giusti, 2007). Red C-PE is the most extensively used colour and has various application in food, cosmetics, and pharmaceutical industries; however, because they are rare in nature, they must be synthesized. Therefore, the food sector is expressing an increasing interest in the finding, application, and stabilizing of natural red colorants, which are currently in high demand. A growing number of commercial companies are turning to cyanobacteria for colour extraction, including Spirulina sp. for the extraction of Phycoerythrin, Haematococcus sp. for the extraction of astaxanthin pigments, and Dunaliella sp. for the extraction of beta-carotene pigments (Sharma et al., 2021).

Bone healing is an extraordinary process involving not only an inflammatory reaction and formation of callus but also reconstruction of the defect to regain its original biomechanical strength. Bone healing can be modified by variety of endogenous or exogenous factors that affect the metabolic function of bone cells. Prostaglandins (PG), a family of cycled derivation of unsaturated long chain fatty acids, are intimately associated with bone metabolism, and are important as locally active agents with significant effects on bone and bone cells in vitro. In some experimental studies, it is suggested that the local increase of prostaglandin in response to trauma may act to stimulate differentiation and proliferation of osteoprogenitor cells during fracture healing, whereas, specific prostaglandin antagonists, indomethacin, inhibited fracture healing. The objective of this chapter is to investigate the effects of locally (defect-site) administered prostaglandin (PGE₂) on cortical bone healing in rabbit model.

Clinically healthy adult New Zealand White rabbits (10) of either sex are divided equally into groups A and B, consisting of 5 animals each. They are kept on standard diet and management throughout the experiment. In all the animals, mid-shaft single transverse fracture is created in both the ulnae under thiopental (2.5%) anaesthesia.

In test group A, 0.1 ml (25 µg) prostaglandin (PGE₂) is injected at fracture site on alternate day for 30 post operative days, whereas, group B is served as control. In both the groups lateral and antero-posterior radiographs are taken on days 0, 10, 20, 30 and 40 postoperatively to observe progress of fracture healing and complication, if any at different intervals.

Abstract

The experiment, conducted at research farm of Central Arid Zone Research Station, Bikaner during 2009 on sandy loam soil. The study revealed that in agri-horti system different irrigation levels significantly affected the growth parameters (plant height, stem girth and tree canopy) of fruit trees which was highest at 80% ETc irrigation level in intercropping of moth bean and cluster bean and it was significantly higher over no intercropping. Intercropping of sonamukhi gave 24.8% lower plant height of citrus over no intercropping. Highest fruit yield of citrus was recorded (40% fruiting) with intercropping of moth bean at 80% ETc irrigation level which was 51% higher over intercropping of sonamukhi.

Abstract

Noodles are widely consumed throughout the world and their global consumption is second only to bread. In the study, response of reducing agents (cysteine hydrochloride and sodium metabisulphite) and oxidizing agents (potassium bromate and benzoyl peroxide) at 10 and 20 ppm levels on the farinographic and amylographic curve characteristics as well as on the noodle making properties of durum and commercial wheat semolina were investigated according to the AACC (1990) procedure. The farinographic curve characteristics revealed that water absorption was enhanced with increasing levels of reducing agents but decreased with addition of oxidizing agents. However there was no significant change in Dough Development Time in case of durum semolina on addition of reducing agents, but commercial semolina recorded significant changes at 20 ppm level of reducing agent. The best reducing agent for durum and commercial semolina was thus sodium metabisulphite and cysteine hydrochloride respectively. Addition of oxidizing agent potassium bromate at 20 ppm level enhanced stability and was thus chosen as the best oxidizing agent for durum semolina and benzoyl peroxide for commercial semolina. Addition of reducing and oxidizing agents improved yield, appearance, elasticity and extensibility characteristics of instant fresh noodles. The best levels were used in the preparation of noodles on the Italian extruder (NAMAD).

This practical in continuation of the practical no.10 explore the effects of screen sizes on different varieties. The screen sizes vary not with crops but with varieties also. To know the effect of screen sizes among the varieties we need to take the top and middle screens as standard or as recommended. These methods can be applied to any crop and to different varieties.

ABSTRACT

Anursery experiment was conducted to study the effect of solarization, selected native antagonists viz., T. viride and T. longibrachiatum and the standard culture of T. harzianum for assesseing the growth enhancement and management of Phytophthora rot in black pepper nursery. The experiment was laid out at College of Horticulture with 20 different treatments and replicated thrice. The sprouting percentage of cuttings in different treatments was worked out at different intervals, which showed significant difference. In general, it was observed that the maximum sprouting percentage was noticed in treatments where solarized potting mixture and biocontrol agents were used. The population of soil microflora viz., fungi, bacteria and actinomycetes in different treatments were also estimated. The study indicated a fluctuation in the population of soil microbes in various treatments at different periods of observation. The observations on height and number of leaves of pepper cuttings in different treatments were recorded. It was noticed that cuttings raised in solarized potting mixture incorporated with native antagonists, especially T. viride, exerted a significant effect in increasing the height of cuttings. With regard to total number of leaves, it was also observed that addition of native antagonists in solarized potting mixture had a positive effect in increasing the leaf production. Thus, the effect of solarization enhanced the growth parameters in crop plants by making available the required nutrients. The increased growth response of plants by soil application of Trichoderma spp. may be due to the production of growth promoting substances by the antagonists. Thus, the nursery experiment revealed that, in general, soil solarization and incorporation of antagonists had a favourable effect in promoting the growth of pepper plants besides checking the Phytophthora rot in black pepper nursery.

Abstract

The treatments showed significant differences for the characters under study in all the years. The maximum curd yield of 186.91, 180.24 and 192.83 q/ha (Av.186.66 q/ha) was recorded during the year 2002,2003 and 2004, respectively with early planting (30th Aug) and closer spacing (45x30 cm)  found significantly superior to other treatments. Similarly, maximum plant height of 75.06, 69.53 and 60.67 cm (Av. 61.47 cm) during the years 2002, 2003 and 2004, respectively was also recorded in the same treatment. Whereas maximum plant spread of 59.13, 78.97 and 65.45 cm (Av. 67.85 cm) was recorded during the year 2002, 2003 and 2004. respectively with second date of transplanting (15th Sept) and wider plant spacing of 45x45 cm. From the results of three year study it can be inferred that the early planting (30th Aug) and closer plant spacing (45x30 cm) is most suitable for obtaining higher curd yield of broccoli under Kashmir conditions.

Abstract

Paddy seeds of variety PR - 116 were obtained from the PAU seed farm. These seeds were cleaned with commonly used seed processing equipment. Samples were divided into 2 parts. One part was kept as such for taking the observations of feed (control) and the remaining part was passed through specific gravity separator for its refinement. The various fractions obtained were recovery, middlings, and rejections. These graded fractions as well as the feed were packed in 5-kg cloth bags and stored under ambient conditions. Different quality parameters viz. moisture content, physical purity, thousand grain weight, percent germination, root/shoot length, vigour index and speed of germination were recorded at 30 days interval. The physical purity, thousand grain weight, percent germination, root length, shoot length and vigour index were found to be higher in recovery and minimum for rejections while moisture content was highest for rejection as compared to all other fractions. Germination, root/ shoot length, vigour index and speed of germination increased with the duration of storage while moisture content, thousand-grain weight and physical purity followed a reversed trend. Compared to feed, the recovery had higher quality attributes after 4 months of storage viz. moisture content decreased by 0.3%; physical purity increased by 0.67%; thousand grain weight increased by 2.97 gm/ thousand grain, germination increased by 8%; vigour index increased by 481.1 points; speed of germination increased by 3.2 seedlings per day. The grading resulted in improvement in overall quality of sunflower seed.

Most of the starch sources are cereals (40 to 90%), tubers (65 to 85%), roots (30 to 70%), legumes (25 to 50%) which contain nearly 70% of starch on dry basis (Santana & Meireles, 2014). Major application of starch is seen in food production, where it is used as a thickening agent, for stabilization, adsorption, gelling, for binding aromatic compounds and as a film forming agent as well (Subaric et al., 2007). About 0.8-3.2 tons of CO2 per ton of plastic can be saved by using starch-based plastics (Patel 1999).

Abstract

Re-constituted rice-premixes made by extrusion, has been successfully formulated for the fortification of market rice with vitamin A (retinyl palmitate and betacarotene) and iron. The statistics of vitamin A deficiency (VAD) and Iron deficiency anaemia (IDA) are alarming in the developing world, particularly in India. In fact, India is second largest producer as well as consumer of rice in the world, so fortification of rice with vitamin A and iron seems to be a successful solution to reduce the VAD and IDA in India. In order to design the final formulation, eight formulations containing target micronutrient profile were tested for vitamin A (both retinyl palmitate and beta-carotene) and iron retention for a period of six months under ambient (24-26 ⁰C) and elevated temperature (40 ⁰C) conditions. Nonsignificant iron loss was observed during six months of storage time under both ambient and elevated conditions. The higher retention of retinyl palmitate and beta-carotene was observed in formulation 5 and 8 respectively. In F5, the retention of retinyl palmitate was 73% and 66% and in F8, retention of beta carotene was 70% and 63% under ambient and elevated temperature conditions respectively after six months of storage. Substituting the original, vegetable shortening with palm shortening resulted in increased retention of retinyl palmitate and beta carotene in formulation 5 and 8. Thus, increased antioxidant effect of natural antioxidants in palm shortenings contributed to stability of micronutrients.

Introduction Noodles have received importance worldwide due to their particular taste, flavor, convenience, consumer acceptance, and low cost. The noodles generally come under the category of unhealthy and junk food because of high carbohydrate content and low vitamins, minerals, and fiber content (Park et al., 2011). Due to the increasing popularity of instant noodles, many people are becoming more prone to various health problems. The substitution of various ingredients to wheat flour noodles is increasing by incorporating different pulses, cereals, and pseudocereals to improve the nutritional properties and also make them health food. Among the pseudocereals, the use of amaranth and chia seeds in food products has been increasing because of their high nutritional value and good health benefits. Their proteins have well-balanced amino acids with a high content of essential amino acids and also good bioavailability (Lopez et al., 2019). These pseudocereals are also gaining importance due to their high content of phytochemicals and other macromolecules. The amaranth (Amaranthus sp) seeds are spherical and small, having approximately 1 mm diameter with a creamish to light reddish color. The composition of amaranth flour includes amylose (7.05%), proteins (15.27%), fat (7.33%), crude fiber (2.04%), and ash (2.93%) (Siwatch et al., 2019).

In India after rice wheat (Triticum aestivum L.) is the second most important cultivated food crop belonging to family poaceae. Total production of wheat was 760 million tons in India during 2016-2017 (FAOSTAT, 2017) which is second largest in the world after china. In India highest producing state of wheat is Uttar Pradesh followed by Punjab, Haryana, Madhya Pradesh, Rajasthan etc. Wheat is categorize into seven commonly known wheat classes those are hard red spring, hard red winter, soft red winter, hard white, soft white, durum and mixed wheat (Khamis, 2014). 

Abstract

A field experiment was conducted at Mechanized Commercial Farm, College of Agriculture, Junagadh Agricultural University, Junagadh (Gujarat) during the Kharif-Rabi seasons of 2006, 2007 and 2008 to study the effect of tillage practices on yield of rainfed castor (Ricinus communis). The experiment was laid out in Randomized Block Design with four replications. The six treatments comprised viz., T_1- Conventional tillage (Cultivator + blade harrowing), T_2-Ploughing followed by cultivator and blade harrowing, T_3-Tillage through rotavator, T_4-Furrow opening through sub-soiling, T_5-Furrow opening through V-notch cultivator and T_6-Zero tillage (pre and post emergence herbicides + 2 hand weeding). Among the different treatments, the different growth and yield attributing parameters viz., plant height, number of spikes per plant, length of main spike, number of capsules per spike and test weight, seed and stalk yields, higher gross and net returns were recorded when field was prepared by ploughing followed by cultivator and blade harrowing. It also improved soil moisture content which was closely followed by tillage through rotavator (T₃) and furrow opening through sub-soiling (T₄).

Bone healing can be modified by variety of endogenous or exogenous factors that affect the metabolic function of bone cells. The Food and Drug Administration is approved the use of low-intensity ultrasound for the accelerated healing of fresh fractures in October 1994 and for the treatment of established nonunion in February 2002. The first regulatory approval is based primarily upon two rigorous, double - blind, placebo controlled clinical trials, which shows that the rate of healing of fresh fracture is accelerated by treatment with ultrasound. Therapeutic ultrasound has been able to improve functional activity, reduce post-ambulatory period, alleviate suffering and restore the animal’s health early. It has been used frequently for acceleration of healing in various musculotendinous injuries in animal. However, the role of ultrasound therapy in different dose levels in fracture healing has not been elucidated properly. In view of the above fact, this chapter is undertaken to investigate the effects of therapeutic ultrasound in two different doses, on cortical bone healing in rabbits.

Twelve clinically healthy adult New Zealand white rabbits of either sex are divided equally into three groups A, B and C, and kept on standard diet and uniform management. In all animals, mid-shaft simple transverse fracture is created in both the ulnae under thiopental (2.5%) anaesthesia. All the animals are administered broad-spectrum antibiotic and analgesic and surgical wounds are dressed daily with povidone iodine and antibiotic cream for 4 days postoperatively. Group A animal is received pulsed ultrasound therapy @ lW/ cm² (1:1) whereas, group C animals is received continuous ultrasound therapy @ 3 W/cm², starting on day 4 after the creation of fracture. Ten treatments, each consisting of 5 min duration, are given on alternate days. Group B animals are served as control. In both the test groups (A and C), the transducer head of the ultrasound machine is moved in a linear fashion along the length of the bone after applying ultrasound gel.

In recent years the importance of VAM fungi for plant growth in most soils has been accepted and acknowledged by soil chemists, agronomists, horticulturists and farmers. Ever since it was shown that VAM could increase nutrient uptake and water uptake from soil in plants, researchers have been trying to extend and manipulate this effect of mycorrhiza in improving the growth and productivity in various groups of plants. Mycorrhizasts of the country have also explored the possibility of utilizing this tool for improving the performance of various crops including cereals, millets, pulses, vegetables, oilseeds and fruit crops. In addition, they have tried to explore the possibility of utilizing this biotool also for improving the growth of various types of trees including forest trees. Following account is related to the work done by the mycorrhizasts of the country on the effect of VA mycorrhiza on the growth and productivity of various groups of plants including cultivated crops.