eChapters

Introduction

Gel electrophoresis is a technique for separating charged molecules with different sizes. Two kinds of gels are commonly used: agarose and polyacrylamide. Agarose gels can be applied to a wider range of sizes than polyacrylamide gels.  By using standard agarose electrophoresis, nuclei acids up to 50 kb may be separated. If Pulsed Field Gel Electrophoresis is used, the upper limit can be extended to 10 Mb. Polyacrylamide gels may separate nucleic acids that differ in length by only 1 nucleotide if their length is less than 500 bp (Sambrook et al., 1989).

Principle

The principle of Electrophoresis involves the motion of a particle in a fluid medium, where the gravitational attraction (on a flowing liquid) is the sedimenting force, and the frictional force is the opposing force and is proportional to the velocity of the particle. These forces balance each other acting in opposite directions and hence balance each other. This will lead to the uniform motion of the particle in the mobile liquid medium (i.e. the particle moves at constant velocity). In case the external field is an electric field instead of a gravitational field, a macromolecule will respond to the external field in two ways. In case the molecule is charged, it will migrate in an electric field to the electrode of opposite charge. Under this principle the technique of electrophoresis functions.

All electrophoresis operations are governed by the principle:

Electrophoresis is an analytical method frequently used in molecular biology and medicine. It is applied for the separation and characterization of proteins, nucleic acids and subcellular-sized particles like viruses and small organelles. Its principle is that the charged particles of a sample migrate in an applied electrical field. An electric current is passed through a medium containing the molecules, and each molecule travels at a different rate depending on its electrical charge, size and shape. Separation by electrophoresis is based on these differences. In electrophoresis, agarose and acrylamide gels are used for electrophoresis of nucleic acids and proteins.

What is Gel Electrophoresis?

A gel is a solid form. The term electrophoresis describes the migration of charged particle under the influence of an electric field. Electro refers to the energy of electricity. Phoresis, from the Greek verb phoros, means “to carry across.” Thus, gel electrophoresis refers to the technique in which molecules are forced across a span of gel, motivated by an electrical current. Activated electrodes at either end of the gel provide the driving force. A molecule’s properties determine how rapidly an electric field can move the molecule through a gelatinous medium.

Electrophoresis is a method of separating out large molecule ( DNA or protein). An electric current is passed through a medium containing the molecules, and each molecule travels at a different rate depending on its electrical charge, size and shape. Separation by electrophoresis is based on these differences. In electrophoresis, agarose and acrylamide gels are used for electrophoresis of nucleic acids and proteins.

What is Gel Electrophoresis?

A gel is a solid form. The term electrophoresis describes the migration of charged particle under the influence of an electric field. Electro refers to the energy of electricity. Phoresis, from the Greek verb phoros, means “to carry across.” Thus, gel electrophoresis refers to the technique in which molecules are forced across a span of gel, motivated by an electrical current. Activated electrodes at either end of the gel provide the driving force. A molecule’s properties determine how rapidly an electric field can move the molecule through a gelatinous medium.

Electrophoresis is the migration of particles under the influence of a direct electric current. There are two requirements for this process to be carried out. First the particles to be separated should be charged or at least should accept charge. Proteins, amino acids, nucleotides etc. conform to this requirement. Second, the medium used for separating the compounds also should carry charge. The mobilities of the ions depend on the nature of the electrolyte solution, the concentration and temperature. The cations and anions move in opposite directions with the cations moving towards the anode and the anions towards the cathode.

Electrophoresis is an important separation technique for the charged molecules. Biologically important molecules, such as amino acids, peptides, proteins nucleotides, nucleic acids which carry electrical charge as they possess certain ionizable functional groups may be separated and identified from a mixture with the help of this technique. Some compounds that are non-polar in nature and do not possess any charge may be transformed to charge carrying derivatives. Thus, phosphate derivatives of carbohydrates may be separated and characterized utilizing electrophoretic techniques.

Introduction 
Building on our extensive exploration of how individual weather parameters intricately influence every facet of crop life and the diverse methodologies employed to evaluate these responses, we now embark on a journey into the mathematical heart of crop science. This chapter, Elementary Crop Growth Models,” will introduce you to the foundational concepts and simpler mathematical frameworks used to quantify, simulate, and ultimately predict how crops grow under varying environmental conditions. In the upcoming sections, we’ll delve into the fundamental building blocks of crop modeling. You’ll discover how these elementary models simplify the complex biological processes occurring within a plant to create manageable, yet insightful, representations. Unlike highly detailed mechanistic models, which require vast amounts of data and computational power, elementary models focus on capturing the most essential relationships between environmental drivers (like solar radiation, temperature, and water) and key aspects of crop growth, such as biomass accumulation or phenological progression. These simpler models serve as a crucial stepping stone. They allow us to grasp core principles of crop-weather interaction in a quantifiable way, providing practical tools for initial assessments, quick predictions, and conceptual understanding, without getting lost in the overwhelming complexity of every single physiological pathway. By understanding these foundational models, you’ll gain a clearer perspective on how agricultural scientists begin to predict the future performance of our food crops, even as the climate around them continues to evolve

Introduction

The basic physiological structures of mammary glands in all species are almost same and are located externally in order to fulfill the milk requirement of neonates after parturition. The process of secretion of milk from the mammary glands is known as lacto genesis. Milk provides an almost complete nutrition to neonates from birth till weaning period. It is a complex mixture of various constituents like:

Lipids such a striacyl glycerols(TAG),phospholipids,cholesterols and other sterols, free fatty acids and fat-soluble vitamins

Carbohydrates such as lactose, oligosaccharides

Proteins such a scaseins, α-lactalbumin, β-lactoglobulin, immunoglobulin’s, bovine serum albumin, lactoferrin, lysozyme, enzymes, hormones and growth factors

Non-protein nitrogenous compounds like urea, creatine, creatinine, uric acid and amino acids

Major and minor minerals, water-soluble vitamins and cells (leucocytes, macrophages, and epithelial cells).

5.1 Elements of Landscape Gardens

5.1.1 Introduction

Elements of a garden are the basic units of any visual design which form its structure and convey visual messages. Elements are based on the art principles of landscaping. Process of landscape design includes concepts and elements of landscape designing. Generally, elements of a garden are divided into 2 types

The plant can absorb more than sixty elements as revealed from the plant tissue analysis, still a few elements is considered essential for the growth and nutrition of the plants. The deficiency of any one of the essential elements can stop completing the life cycle of the plants which is reflected on the yield of the crop. The growth of the plant which is expressed in terms of dry weight, length ,diameter etc. are interlinked with the plant available nutrients of soils.

3.1 Essential Nutrients

The essential elements are those elements which are required for growth and nutrition of the plants. There are 17 elements known to be essential nutrients for plants. The essential elements are classified as micro and macronutrients depending upon the requirement of the crop. Among the naturally occurring elements, eighteen elements are considered essential to plants. These are: C, H, O, N, P, K, Ca, Mg, S, Fe, Mn, Zn, Cu, B, Mo, Cl, Ni, Co. The elements which are found in different sources are required to be in available forms (Table 3.1) for growth and nutrition of the plants.

A. Answer the following.

Q.1.     What are the different phagocytic cells ?
Ans.     The phagocytic cells of mammals belong to two complementary systems (1) The myeloid system consists of cells that are rapidly phagocytic but incapable of sustained effect. (2) Mononuclear phagocytic system act more slowly but capable of repeated phagocytosis.

Q.2.     How 3 types of granulocytes are differentiated ?
Ans.    Three types of granulocytes are distinguished by the staining property of their granules. Cells whose granules take up basic dyes such as haematoxylin (a blue dye) are called basophils, whose granules take up acidic dyes such as eosin (a red dye) are called eosinophils and those that take up neither basic nor acidic dyes are called neutrophils.

Elephant foot yam, also known as suran or jimmikand, is gaining popularity due to its high yield potential and culinary qualities. It is grown in Andhra Pradesh, Bihar, Gujarat, Kerala, and Maharashtra in India, as well as in the north-eastern states of Odisha, Tamilnadu, Uttar Pradesh, and West Bengal. This crop is being embraced for commercial production in northern and eastern India, thanks to the advent of a high yielding, non-acrid variety. Elephant foot yam has an output potential of 50-60 tonnes per hectare. Elephant foot yam is a tropical tuber crop that has been grown for generations in tropical Asia. It is gathered for its edible corms, tubers, and smooth petioles, which all contain important minerals.

Elephant foot yam is an underground stem tuber belonging to the family Araceae, one of the most popular tuber crops, extensively used as a favourite vegetable by millions of people in India. It has both nutritional and medicinal value and is usually consumed as cooked vegetable. It has high dry matter production capability per unit area than most of the other vegetables. Elephant foot yam is a remunerative and profitable stem tuber crop. The crop is gaining popularity due to its shade tolerance, easiness in cultivation, high productivity, less incidence of pests and diseases, steady demand and reasonably good price.

5.1 Introduction

In recent decades, human induced changes in climate of the earth have the focus of scientific and social attention. The most imminent of this is the increased concentration of greenhouse gases (GHGs) namely, carbon dioxide (CO₂), methane (CH₄) and nitrous oxide (N₂O) in the atmosphere. The concentration of CO₂, CH₄ and N₂O have increased markedly by 30%, 145% and 15%, respectively as a result of human activity since 1750, the era of Industrial Revolutions (IPCC, 2007). Between 1750 and 2005, the concentration of CO₂, CH₄ and N₂O in the atmosphere has increased from 280 to 379 ppm, 715 to 1774 ppb and from 270 to 319 ppb, respectively (IPCC, 2007). However, there was a steep increase during the recent years i.e. 70% increase of GHGs between 1970 and 2004.

Immunoassays rely on the ability of an antibody to recognize and bind a specific macromolecule in what might be a complex mixture of macromolecules. In immunology the particular macromolecule bound by an antibody is referred to as an antigen and the area on an antigen to which the antibody binds is called an epitope.

In some cases an immunoassay may use an antigen to detect for the presence of antibodies, which recognize that antigen, in a solution. In other words, in some immunoassays, the analyte may be an antibody rather than an antigen.

As aquaculture expands rapidly across states like Andhra Pradesh, Tamil Nadu, Kerala, and West Bengal, the demand for accurate and timely disease detection methods has intensified. Enzyme-Linked Immunosorbent Assay (ELISA) and other serological tools have emerged as critical assets in pathogen surveillance and management in fish and shrimp farming systems.
ELISA, widely used in Indian diagnostics laboratories, enables the detection of pathogen-specific antigens or host antibodies with high sensitivity and throughput. Its capacity to analyze multiple samples simultaneously and deliver quantitative results makes it particularly suitable for large-scale disease monitoring. Both indirect and sandwich ELISA formats are employed, tailored to the diagnostic goal—whether it is detecting the pathogen itself or assessing the host’s immune response.
In indirect ELISA, fish serum samples are screened for antibodies against key pathogens. This format is instrumental in vaccine efficacy studies and epidemiological surveys. For instance, aquaculture centers in Kerala and Tamil Nadu use indirect ELISA to track immune responses to Aeromonas hydrophila and Koi Herpesvirus (KHV) in ornamental and carp species. On the other hand, sandwich ELISA is utilized for direct antigen detection in tissues and fluids, and is applied in shrimp hatcheries for diseases such as White Spot Syndrome Virus (WSSV) and Yellow Head Virus (YHV), supported by ICAR and MPEDA programs.
Other serological techniques also find relevance in Indian aquaculture. Agglutination assays, especially slide agglutination, offer rapid and cost-effective field diagnostics. These are applied in the detection of Edwardsiella tarda and Streptococcus iniae in tilapia and catfish. Latex agglutination further enhances rapid bacterial identification at the farm or lab level. Immunodiffusion techniques, such as Ouchterlony double diffusion, though less sensitive, are still used in resource-limited areas for detecting fungal pathogens like Ichthyophonus and Saprolegnia.

Teaching and research in anatomy is mainly based on cadaver dissections. A sound knowledge of anatomy is essential from the beginning of veterinary education and the knowledge obtained through dissection of animal body is an indispensable part of the veterinary science.
History of embalming
Embalming is the art and science of preserving the animal by treating with chemicals to forestall decomposition. Embalming destroys the colloid nature of the proteins and neutralizes the active centres of the molecules forming many cross linkages of adjacent protein molecules. Proteins are thus converted to high molecular inert solid materials that can no longer serve as food for bacteria or as a substrate for enzyme action. Embalming preserves the body from decay. The embalmed bodies are stored in tanks containing a fluid composed of formalin, phenol, thymol and rock salt in water in varying proportions.
Claudius Galen (130-200 A.D) did dissections of animals such as the pig or monkey. Leonardo Da Vinci (1452-1519) a pioneer anatomist introduced anatomical drawings. During 15th-16th century the motive was to advance the techniques for the preservation of the dead and to study detailed anatomy by dissections. Andreas Vesalius (1514-1564) believed that dissection was necessary to find out how bodies worked. His lectures were based purely on his observations. He carried out dissection on human cadavers as well as animals to understand Anatomy. As an Anatomist he represents the end of the middle ages obscurantism and the beginning of modern Anatomy and scientific thinking. He is considered the father of modern Anatomy. In 17th century Antonie Van Leeuwenhoek (1632-1723) opened up new horizons

8.1 Design of Canal Embankments

 There are two aspects of canal embankment design.

 (i) The general requirement, such as free board, dowel, service/inspection road, drainage of banks etc. The aspect of free board is discussed in Para

8.2 to 8.3 and that of service/ inspection road in Para

 8.4 and drainage of banks in Para 8.5 in this chapter.

 (ii) Safe design of embankment i.e. Hydraulic and structural requirement.

It includes stability of inner and outer slopes, Structural safe design of inner cut slopes in deep cuttings in different types of soils/rocks of varying nature and ground water conditions. Stability of slopes may include drainage, so as to prevent rock falls/debris in canal. For outer slopes in embankment (filling sections) hydraulic gradient/pharetic line, toe drains etc, similar to the earthen dams, on different types of soil/rock foundation with respect to varying ground water level conditions are important.

The canal embankments in filling may even fail through its foundations by sloughing or liquefactions. All these aspects of canal embankments are discussed in this chapter in Para 8.4 to 8.9.