eChapters

Terminology

    ·    Nutrient: Nutrients are substances required by an organism for their normal     growth and reproduction. 
    ·    Plant Nutrient: The plant nutrient is a “food” which is composed of certain chemical elements often referred to as ‘plant nutrient’ or plant food elements considered very essential for growth and development of plants. 
    ·    Nutrition: The supply and absorption of chemical compounds needed for growth and metabolism of an organism.

Agricultural meteorology, a discipline that has traditionally focused on understanding the relationships between weather and climate, and agricultural productivity, is at a critical juncture. The increasing vulnerability of agricultural systems to climate change, coupled with the need for sustainable and resilient agricultural practices, necessitates a fundamental transformation of agricultural meteorology. To support the development and implementation of climate resilient agriculture (CRA) practices, agricultural meteorology must be reoriented as a super speciality discipline. This requires a comprehensive overhaul of the discipline, including its curriculum, research focus, and practice. The reorientation of agricultural meteorology as a super speciality discipline is essential to provide the necessary expertise and knowledge to support CRA practices. This includes developing and applying cutting-edge technologies, such as precision agriculture, remote sensing, and machine learning, to support agricultural decision-making. However, the current state of agricultural meteorology is not equipped to meet the demands of CRA. The discipline faces several challenges, including a lack of interdisciplinary approaches, inadequate emphasis on practical skills, and limited focus on climate change and sustainability (Fig. 8.1). To address these challenges, essential reforms are necessary to reorient agricultural meteorology as a super speciality discipline. This chapter will discuss the essential reforms required to transform agricultural meteorology, including reforms in curriculum, research, practice, and policy. By implementing these

Introduction

An entrepreneur is someone who starts or runs their own business. By having an equity stake in the company, the entrepreneur can profit greatly if things go well; however, they also take on a great deal of risk-far more than a regular employee of the company. Financial, career, emotional, and overall business risks are all examples of entrepreneurial risk. Because there is so much at stake in starting and growing a successful business, there are very specific skills that an entrepreneur usually requires to be successful. It could be a picture of a talented college dropout or a seasoned business professional with an uncanny ability to predict the next big thing. Replace whoever the persona is with ourselves. A successful entrepreneur does not have a specific demographic or personality profile. We can be entrepreneurs regardless of our age, race, gender, sexual orientation, socioeconomic status, or upbringing if we have dedication, drive, and essential business skills. Unlike personality traits and demographic information, entrepreneurial skills can be learned and practiced. If we want to pursue entrepreneurship, use this list to assess our strengths and weaknesses and determine which skills we need to develop before launching our venture.

This chapter outlines fundamental molecular biology protocols essential for genomic analysis and molecular characterisation. Modern molecular biology relies heavily on precise analytical techniques for studying genetic material. Hence, this section presents a typical workflow encompassing nucleic acid extraction, amplification, and subsequent analysis. These methodologies are fundamental to various applications in molecular biology, including phylogenetic analysis, gene expression studies, and molecular diagnostics.
The chapter covers four core molecular biology and bioinformatics techniques, each building upon the previous:
1. Genomic DNA Extraction: Protocols for efficient isolation of DNA from biological samples. This encompasses cell lysis, protein denaturation, and nucleic acid purification through standard separation techniques. The quality and purity of extracted DNA significantly influences downstream applications.
2. Primer Design with Primer3: In silico design of oligonucleotide primers for PCR amplification. This section covers primer design parameters including optimal melting temperature (Tm), GC content, primer length, and considerations for avoiding secondary structures and primer-dimer formation.

At times, we hear our young generation questioning the need for obtaining degrees for conducting any research work. To some extend they are right. If we look back, there are many examples where persons without any former degree attained what is impossible by many degree holders. Tulsidas has written ‘Ramacharitmanas’, incorporating so many principles of Vedas and stories from Puranas without obtaining any degree. Srinivasa Ramanujan (21) narrated mathematics theorems without any degree in mathematics. His achievements may be appreciated by the fact that we celebrate his birthday 22nd December as National Mathematical day.

To set up a soil-testing laboratory the minimum requirements are as follows:

8.1 Laboratory floor area

Minimum four rooms are needed. They are as follows:

1. Office room, floor size: 18’x 12’

This room is used for registration of the soil samples received, recording recommendations along with soil test results in laboratory book and in report sheet, their dispatch, accounts etc.

The establishment of a technology transfer office is an important and complex process for an organization. It needs careful planning to ensure its long-term sustainability. Before establishing a TTO, it is essential to evaluate whether institution’s core mission includes research commercialization, and if the volume and quality of its research output justify the need for an inhouse TTO. For institutions that may not meet all criteria, those institutions can collaborate or outsource for TTO services. It is also important to decide about its operational scope, funding, management, and for what kind of issues the office should develop a policy. There is no “right” way to set up a TTO, but successful TTO does require considering some key issues. This chapter outlines key steps and considerations for establishing a TTO. It also explains what physical infrastructures are needed, manpower, outlines the responsibilities and powers of TTOs.
2.1 Evaluating the need for a TTO
Establishing a TTO is an important step for any research focused organization. But before initiating a planning process for a new TTO, the organization must review its relevance by considering a few fundamental points:
Key considerations for institutions
1. Is institution’s mission focused on “research commercialization” If an institute’s primary mission is education, or it does not support research it might not be justified to create a TTO. For such institutions, it usually makes better sense to seek alternative solutions to the occasional technology transfer without the expense of a formal office. But institutions with strong research commercialization focus, a TTO has the ability to contribute meaningfully to the mission. The Association of University Technology Managers (AUTM), USA has identified following key motivations for initiating academic technology transfer:

1. SEED TESTING

The science of evaluating the planting value of seed has been developed to achieve the following objectives for minimizing the risks of planting low quality seeds.

To determine the seed quality and its suitability for planting

To identify seed quality problems and their probable cause.

To determine the need for drying and processing, if any.

To determine if seed meets established quality standards for labeling.

To establish quality and provide a basis for price and consumer discrimination among lots in the market.

Layout of an orchard

Layout means the way by which something is arranged or laid out. The purpose of designing a layout for an rchard is to assign the actual position for trees/ plantations, roads, water channels, buildings etc. The layout should be planned very carefully and implemented properly to facilitate proper care and maintenance of an orchard. The plan should provide optimum number of trees per unit area as well as sufficient space to each of the tree for proper development and ease in various cultural operations of the orchard.

Materials required for layout

Measuring tape, rope, pegs, poles and planting board.

Based on agro-climatic conditions, the following system of planting are generally followed for planting of fruit trees:

1. Square
2. Rectangular

An orchard is the place where different kinds of fruit crops are grown in large scale. An orchard is a long-term investment and needs lot of planning and expertise. The orchard crops are generally trees, which ultimately have a large spread, so when they are planted, the distance between plants and rows is kept keeping in mind the final spread of the tree. So, before the trees attain their full growth, some suitable crops which are of short duration, like vegetables, flowers, spices and herbs are grown as intercrops.

Fruit trees are perennial, may be tropical, sub tropical and temperate types and thus require proper environmental conditions and suitable management practices for their successful growth.

ABSTRACT

Haploids are of great importance in breeding programmes for the production of homozygous diploid lines. This technique reduces the time span in developing new varieties and fixes the traits including recessive ones in the homozygous condition. The haploid embryos or doubled haploid plants could be used in mutation breeding, genetic engineering, biochemical and physiological studies. Isolated microspore culture for the production of haploids has not yet been reported in Hevea brasiliensis. Hence the present work was initiated with the view of producing haploid plants by the culture of isolated pollen grains. The flower bud of the clone RRII 105 at the late uninucleate to early binucleate stage was used for culture initiation. Different pretreatment solutions were tried at different temperatures (4°C, 27°C and 33°C) with the macronutrients of N6 media. Three kinds of callus induction media were used viz. N6, WPM and Hunters medium supplemented with myoinositol (200 mg^-1), glutamate (150 mg^-1), phenyl acetic acid (7.0 mg^-1), sucrose (70 g^-1) and growth regulators. Induction medium include moving liquid, static liquid and semi- solid medium. The ovary co-culture was also tried in the induction medium to increase the frequency of callus formation. Pretreatment of the anther with 0.3 M mannitol at 33°C promoted the division of microspores. Static liquid cultures improved the formation of multicellular microspores compared to semi-solid or moving liquid cultures. N₆ basal medium supplemented with 2.0 mg/l 2.4-D and 1.0 mg/l KIN induced microcalli formation. When the ovary co-culture was included in the callus induction medium, the rate of cell proliferation was increased considerably and the duration of callus formation was reduced from 3 months to 14 days. The results of the present study throws light towards the possibility of regenerating doubled haploids in future.

Selection of Site and Land Preparation

Cashew is a hardy plant; it thrives well on wide range of soil. While selecting the land for cashew, soils with water logging and excessive salinity of alkalinity should be avoided. Otherwise, soil depth, slope, stoniness, soil fertility and water availability seem to impose very little limitation. For establishing new plantations, land preparation should begin with the first pre-monsoon rains. The area should be cleared of bushes and other wild growth before digging pits for planting.

Planting Season

Planting of soft wood grafts is usually done at the beginning of monsoon season (June-July). Therefore, land preparation such as clearing of bushes and other wild growth, digging of pits for planting, should be done during pre monsoon season (April-May).

The concentration of essential elements must be maintained within quite narrow limits if the growth, health and productivity of the animal are to be maintained. Soil-plant-animal relationship is based on micro-mineral profile in animal blood, plant (fodder) and the soil is of paramount importance to identify dietary requirements of animals maintained under different husbandry practices of various agro-climatic zones. The soil occupies a strategic position in the chain of nutrition flow to plant, animal and eventually to man. The soil is the principal source of trace elements entering the food chain. The influence of the chemical composition of soil on the trace element contents of pant is well known.

1. INTRODUCTION

Field run seeds obtained from the seed production field posses high moisture and physical contaminants like trash and other inert materials (sand, stones, soil etc.,), diseased and pest damaged seeds, other crop seeds, weed seeds, deteriorated and damaged seeds, off-size and ill filled seeds, plant remnants etc. Seed processing is must to dry the seeds to safe moisture level, remove unwanted and inert materials as detailed above.

Seed qualities such as vigour and viability are improved by certain physical characteristics of the seed i.e., shape, size, large, denser and sound seed, etc. So, if grading is done to obtain a particular range of size, shape, length and density of the seeds, the quality of the lot is upgraded.

Seed processing refers to all the steps necessary for preparation of harvested seed for marketing namely handling, drying, shelling, preconditioning, cleaning, size grading, treating and packaging.

Extent of Passage
(Method of Krishna and Ekern, 1974D)
Definition of Terms
The term  extent of passage is used to describe how far a given ingested material travelled  or was allowed to pass. The point at which  undigested residue of the food comes to or has passed through the alimentary tract may be found by the use of markers without disturbing the subject. In some conditions of digestive system malfunction or disorder , the food residue may be blocked at point along with digestive tract.

Transit Time
This is the time it takes the digesta of a meal to pass through the alimentary tract or segments of it. This time also represents the  retention time in the tract or the particular segment. A simple way of calculating it is by recording the time of first or last appearance of the marked residue of a meal. Other more useful methods for calculating retention time include “mean retention time” (Castle 1956a), “mean time” (Blaxter et al., 1956) and “turnover time” (Hungate, 1968).

The damage to food grains in the storage may be caused due to insect attack, mould, fungi, bacteria, rodents, heat and moisture. The value of grain for milling purposes and human food is directly correlated with the amount of insect contamination present. The immature stages of rice and granary weevils, lesser grain borer, Angoumois grain moth and occasionally the flat grain beetle, cadelle, rust red flour beetle and saw toothed grain beetle live inside the kernels where they are hidden from view until they emerge.

Grain from casual inspection appears to be in good condition but may contain enough internal infestation which render it unfit for human food or unfit for purchase for future sale as milling stock. In general, millers have found that wheat with more than 0.5 per cent of infested kernels is unfit for milling. The need for a rapid and accurate method of estimating insect contamination in grain offered for sale has become more and more important to both grain dealers and millers.

Methods for Estimation of Damage

In order to assess the damage caused to food grains due to any one or combination of various factors; physical, chemical and micro-analytical methods are employed according to the necessity and information required. Various methods have been employed by field workers as well as by laboratory technicians in estimating damaged grains in food grains with particular reference to detection of insect infestation which alone cause maximum damage. These methods have been described below.

Principle

The portion of the total ash which is insoluble in dilute hydrochloric acid is called acid insoluble ash (AlA) and it is the part of ash which is having unavailable mineral matter like silica and sand. Silica is the part of mature plant materials which are highly lignified or woody like straw, husk, stems and stalks, while sand inters in feeds by faulty handling in the fields or as an adulterant.

Equipment/glass wares
    1.    Asbestos sheet
    2.     Filter paper (Whatman No.1)
    3.    Funnel stand
    4.    Glass funnel
    5.     Glass rod
    6.     Hot plate/heater/Bunsen flame
    7.     Metal tongs
    8.     Silica basin (crucible)
    9.     Volumetric flask
    10.     Wash bottle with distilled water

Nutritional significance of tannins in feeds and fodders

Tannins are widely distributed in many types of browse plants, tree leaves, forages and feed ingredients. The structure of tannins are not completely well defined but they are polymers of phenolic and aliphatic hydroxy compounds. Although tannins and related polyphenols are considered as protein binders and, protease and cellulolytic inhibitors, they can also exert beneficial effects on rumen fermentation. There are two forms of tannins viz., hydrolysable and condensed. Hydrolysable tannins are considered to have less deleterious effects on protein digestion since these types of tannins might get hydrolysed under acidic gastric condition and release bound proteins. Attempts have been made to utilize this to advantage in protection of proteins from rumen degradation.The condensed tannins are resistant to hydrolytic degradation. The reduction in digestibility due to condensed tannins is not clear whether it is due to specific interference with cellulases or precipitation of proteins.

Certain feed ingredients have been incorporated as a source of tannins, in the diets of ruminants to benefit ruminal fermentation and performance. Inclusion of Bengal gram husk as a source of tannins in the diet of sheep prevented potentially digestible carbohydrate in the rumen, but digested post ruminally (Sreerangaraju et al., 2000).

29.1. Aim

To estimate ascorbic acid in fresh and processed fruit and vegetable products.

29.2. Theory and Principle

Fruit, vegetables and their products are important sources of ascorbic acid. The ascorbic acid is present in sufficient quantity in aonla, guava, grapefruit, lemon, pineapple, strawberry fruits etc. Barbados cherry is the chief source of vitamin C. The products manufactured from these fruits are also considered as rich in ascorbic acid and the contents available in the commodities can be detected by using 2, 6 dichloro-phenol - indophenol visual titration method.

25.1. Aim

To estimate the ash content in fresh and processed products.

25.2. Theory and principle

Estimation of ash contents is important as it gives an idea about the presence of minerals in the food samples. Determination of ash is also required to evaluate fruit chutneys as the maximum limit for ash in this product shall not exceed 5% as per FPO specifications.

25.3. Apparatus/instruments/equipment

1. Crucibles

2. Muffle furnace

3. Sensitive balance

4. Heating plate

5. Desiccators