eChapters

The essential database for all skin diseases consists skin scrapings. Skin scrapings fall into two main categories: superficial and deep. Superficial scrapings give information from the epidermis’s surface without causing capillary bleeding. Capillary haemorrhage is a sign that the sampling was deep enough to gather material from the hair follicle. Deep skin scrapings are used for this purpose. The main purpose of skin scrapings is to detect the presence or absence of mites. A skin-scraping spatula, which is a thin metal weighing spatula, is recommended for use while scraping the skin. These spatulas are safe and can be used again. Scrapings from active lesions at several places are to be submitted. Scraping from active lesions at several places is to be submitted. Generally, the periphery of the lesion contains more parasites than the centre. Skin scrapings are used to identify ectoparasites and fungal diseases.

A. Intestinal protozoa Fresh faeces must be used as a sample. The sample must be examined immediately and without refrigeration. Methods of examination 1. Direct smear examination A thin smear of faeces mixed with either water or normal saline (recommended for Trophozoites since they stay intact and motile longer). Select blood-tinged mucus or tiny tissue bits if the faeces are dysenteric. One to two percent of an aqueous eosin solution can be used in place of saline or water, but the oocyst still resist the stain. The viability is examined using the eosin solution. It is possible to employ an iodine solution (which kills protozoa), which allows for the identification and differentiation of the protozoa. It is possible

Purpose of study An essential component of the diagnostic process in the evaluation of the disease process is the laboratory diagnosis of any parasitic infection. Sometimes, this confirms the presumptive clinical diagnosis, and other times, it provides evidence that the clinician may have ignored. A sufficient specimen must be used for the examination to achieve a satisfactory diagnosis. Internal parasite testing needs to be done often. Regular inspection typically enables early parasitism identification, preventing gross infection. The majority of the parasites can be accurately identified by doing a detailed examination of the faeces. Faecal sample collection and delivery for parasite identificatio

Collections of blood To collect blood for different haematological analyses, an animal’s vein are punctured with a syringe and needle. Requirements • Hypodermic needle • Syringe • Collection vials or tubes containing anticoagulantsProcedure • The place of blood collection should always be cleaned with alcohol and hairs trimmed. • Press on the vein to raise it. • Free-flowing blood can be collected directly into the vial containing anticoagulant after the needle is inserted into the vein. • 5 ml of blood from large animals and 2.0 ml of blood from small animals like dogs and cats are sufficient for normal haematological investigations. • Rotational motions in the vials are used to mix the blood

Urinnalysis An essential laboratory test that is easily conducted in veterinary settings and is included in a basic database is urinalysis. Documenting diverse urogenital tract conditions is helpful, because it could reveal details about other systemic conditions including hemolysis and liver failure. Cystocentesis, urethral catheterization, or simply voiding can all be used to collect urine. Urine should be examined within 30 minutes, but if this is not possible, it can be refrigerated for up to 24 hours or sent to a outside diagnostic lab; this, however, may cause crystal precipitation. The pH or specific gravity of urine are unaffected by refrigeration. Preservative should be added if cytologic analysis is necessary and a delay in slide preparation is anticipated. A few drops of 10% formalin (exact concentration not necessary) are better than boric acid

Creatinine Clinical significance Creatinine is produced at a steady rate due to muscle catabolism and is not reabsorbed by the kidney tubules after filtration. Its measurement provides an indirect assessment of the glomerular filtration rate (GFR). Unlike urea it is not markedly affected by diet or any aspect of liver function and therefore the test is more specific for renal dysfunction

Sodium Clinical Significance Compared to potassium, sodium is distributed differently throughout the body. Due to the sodium pump mechanism, most sodium is extracellular. Compared to this, just 2% of potassium is extracellular; the remainder is intracellular. Regarding the plasma concentrations of sodium and potassium, renal function is the single most significant homeostatic mechanism.

Calcium Clinical Significance Calcium is an essential mineral which is involved in many body systems. These include the skeleton, enzyme activation, muscle metabolism, blood coagulation and osmoregulation. In the blood calcium exists as 50% ionized, 40% protein bound and 10% complexes with anions such as citrate and phosphate. Only ionized calcium is biologically active in bone formation, neuromuscular activity, cellular biochemical processes and blood coagulation. Factors governing the total plasma concentration are complex and include interaction with other chemical moieties, proteins and hormones. Calcium, phosphorus and albumin metabolism are interdependent.

A. Triglycerides Clinical significance To form lipoproteins, triglycerides combine with cholesterol, phospholipids, and plasma proteins. To determine whether hypertriglyceridemia may be present, the serum triglyceride concentration is measured (increased blood and serum levels of triglycerides). Chylomicrons and very low density lipoprotein (VLDLs), which cause the extensive lipaemia frequently observed in serum or plasma samples, are both mostly composed of glycerides.

The liver produces the entire albumin and most of the globulin; a small amount of gamma globulin is produced by retculoendothelial tissue. The production of albumin is subject to a mechanism regulating colloidal osmotic pressure and the secondary to alterations in globulin concentration. The liver synthesizes lipoproteins, glycoproteins, mucoproteins, fibrinogen, prothrombin and clotting factors VII, VIII, IX and X.

Clinical Significance The primary aim of laboratory diagnosis is to assess the carbohydrate metabolism impairment in diabetes mellitus, inducing the following: 1. Determination of glucose concentration in fasting blood and demonstration of postprandial glycosuria. 2. Assessment of glucose tolerance

Collection of Blood Objective: To diagnose the existing disease condition, if any. Types of blood collection • Arterial sampling: In order to measure arterial blood gases, an arterial blood sample is taken from an artery. Only veterinary professionals who are legally permitted to do so for their work in their country and who have proven their expertise via formal training should take arterial blood samples. • Venipuncture sampling: The most used method of taking blood from humans and animals is venipuncture. Collection occurs from a superficial vein near the surface that is not very close to any large nerves.

Future scope of the Research

The present study has been conducted by driving the insights deeper into the nature, intensity and character of the problems creating barrier against the farmers happiness and there mental health. The present study thus leaves behind the following domains to be researched out in future:

The study can be extrapolated to other domains of mental health and happiness:

The present study has been conducted in one of the agro-ecological zones of the state of West Bengal, New alluvial zone. Four villages from Haringhata block in Nadia district were selected from the new alluvial agro- ecological zone to conduct the study. One hundred farmers were selected from the villages of Dalilpur, Mitrapur, Fatehpur, 25 respondents from each. The primary data were collected with the help of structured interview schedule by following the personal interview method. The secondary data were collected from our departmental library, internet, Department of Agriculture, West Bengal, Bidhan Chandra krishi Viswavidyalaya, etc. for establishing the conceptual framework of the study.

The chapter deals with the Results and discussion of the study, whose purpose is to provide sufficient information so as to arrive at a valid conclusion. Tables and Figures are commonly used in this chapter to support and supplement textual materials. Discussion helps to interpret the Results of the study from a proper perspective and to relate them with other relevant studies.

Table 6.1. Descriptive Statistics of Variable with respect to Minimum, Maximum, Mean, Standard Deviation of Values, Variance, and Coefficient of Variance:

Research methodology is a detailed plan of investigation and the blueprint of procedure for carrying out the research. In this chapter, a discussion on the methodology has been made to understand the concepts, methods, and techniques, which are utilized to design the study, collect information, analyze data and interpret the findings for the revelation of truth and formulation of theories. The entire chapter has been broken up under the following sub-heads for easy understanding:

1. Locale of research
2. Sampling design
3. Pilot study
4. Variables and their measurements
5. Methods of data collection
6. Statistical tools used for the analysis of data

4.1 Research Setting

In any social science research, it is hardly possible to conceptualize and perceive the data and interpret the data more accurately until and unless a clear understanding of the characteristics in the area and attitude or the behavior of people is at commending by the interpreter who intends to unveil an understanding of the implication and behavioral complexes of the individual who lives in the area under reference and form a representative part of the larger community. The socio-demographic background of the local people in a rural setting has been critically administered in this section. A research setting is a surrounding in which input and elements of research are contextually imbibed, interactive, and mutually contributive to the system performance. The research setting is immensely important in the sense because it is characterizing and influencing the interplays of different factors and components.

This chapter deals with some important terms and their concepts related to the study which are essential to convey the ideas of the study effectively and also to provide a theoretical base for the empirical investigation and guidance for the selection of relevant predictor variables.

3.1 Human Happiness and Estimation

A state of mental or emotional wellbeing known as happiness is characterized by pleasant or good emotions, which can range from contentment to ecstatic delight. Happy emotions can also be a reflection of how someone feels about their general well-being.

This chapter is an extensive survey of all past studies relevant to the field of investigation which gives knowledge of what others have found out in the related field of study. In any scientific investigation or research, a comprehensive presentation of the review of literature is a statutory requirement. Its main functions are to develop a better understanding of the problem to be investigated, delineate a new area of study and avoid unnecessary duplication, decide on the tools and techniques to be adopted including developing some new ones, also to relate the present study with the previous ones by finding out the areas of agreement and disagreement.

The review of literature of the present study is based on the major concepts related to the field of investigation. As direct references on the entire item are not in abundance, certain specific references along with some indirect references have been incorporated in this chapter for the purpose of meaningful use.

Happiness is the barest minimum to any person for survival and moving with a humble vision and mission either. Unfortunately, our farmers are denied to a glow of happiness, and mantled with clouds of agony, depression and confusuion. They are supposed to ensure food security to all, but themselves have to deal with extreme food insecurity, market uncertainty, undulating income, erosion of natural resources and stereotypes of farming occupation. That is how there has been a sharp rise in the migration from farm to off farm occupation.

This is the need of the hour to estimate their happiness. Agriculture is India’s backbone, employing half the country’s population. However, it has been confronting with several issues, including unfair and non-remunerative market prices, an unstructured supply chain, disaster driven crop failure, and so on. However, an alarming trend in our country shows that farmers are desiring to quit agriculture by large number, 45-60 per cent, and are committing suicides; this is a threat to our long-term a gri-development. Do they cultivate because they desire to, or because they have got no other choices either?