eChapters

11.1 Introduction

In the preceding chapter 10, we have discussed in detail, the test procedures for which it has been assumed that the sample size n is large, (i.e n >,30). Those tests are also known as approximate tests or z tests. But in case when sample size n is small i.e. n<30, the approximate test fails to deal with such problem and we apply the theory of exact sample, for small sample tests. The exact sample tests however can be applied to large samples also though the converse is not plausible. In all the exact sample tests, the basic assumption is that the parent population is normally distributed.

In this part we will discusse in details the exact sample tests (based on exact sampling distributions namely snedekor’s F, student’s t and chi square (c²) distributions. These distributions will be simply introduced in short because main emphasis has to be given only on the test procedures based on them.

11.1 Introduction
In the preceding chapter 10, we have discussed in detail, the test procedures for which it has been assumed that the sample size n is large, (i.e n >,30). Those tests are also known as approximate tests or z tests. But in case when sample size n is small i.e. n<30, the approximate test fails to deal with such problem and we apply the theory of exact sample, for small sample tests. The exact sample tests however can be applied to large samples also though the converse is not plausible. In all the exact sample tests, the basic assumption is that the parent population is normally distributed.
In this part we will discusse in details the exact sample tests (based on exact sampling distributions namely snedekor’s F, student’s t and chi square (χ2) distributions. These distributions will be simply introduced in short because main emphasis has to be given only on the test procedures based on them.

14.1 Section-A : Write True / False

Q.1 If sample size n is small ie n < 30, then the small sample test is applicable.

Q.2 The exact sample test (small sample test) can be applied to large sample test but the converse is not plaussible.

Q.3 If (x²1 , x²2 ) are two independent chi-square variates with (V1, V₂) degree of freedom then F statistic is def ned as

• General considerations

 • Blood glucose

• Total serum protein

 • Serum albumin

 • Serum globulin

 • Serum gamma globulin

 • Serum calcium

 • Serum inorganic phosphorus

 • Serum magnesium

 • Serum cholesterol

 • Serum creatinine

 • Serum urea

 • Serum chloride

 • Serum sodium

 • Serum potassium

 • Serum Glutamic Oxaloacetic Transaminase

 • Serum Glutamic Pyruvic Transaminase

 • Serum alkaline phosphatase

Collection of blood Blood sample should be collected in a capped clean and dry vial containing an appropriate amount of anticoagulant. The commonly used anticoagulants are ethylene diamine tetra acetic acid (EDTA) @ 1-2 mg/ml and heparin @ 5 I.U./ ml of blood. Sites for blood collection Before collection of blood clean the site of collection by applying some antiseptic solution. If the area is hairy shaving of the site under aseptic condition should be done. Tip of the ear should be preferred for prompt or early diagnosis of haemoprotozoan infections. The vein can then be punctured with hypodermic needle and the collected blood is transferred to a clean glass vial. For collection of blood various preferred sites in different animals are as under

Collection of blood

Blood sample should be collected in a capped clean and dry vial containing an appropriate amount of anticoagulant. The commonly used anticoagulants are ethylene diamine tetra acetic acid (EDTA) @ 1-2 mg/ml and heparin @ 5 I.U./ml of blood.

• General considerations

 • Collection of blood

 • Storage

 • Examination of blood

 • Coagulation time

 • Bleeding time

 • Determination of movement of parasites under wet film

• Total erythrocyte count

 • Total leucocyte count

 • Differential leucocyte count

 • Hemoglobin

 • Erythrocyte sedimentation rate

• Hematocrit value

 • Mean corpuscular volume

 • Mean corpuscular hemoglobin

 • Mean corpuscular hemoglobin concentration

 • Erythrocyte fragility test

• General considerations

 • Indications

 • Limitations

 • Techniques of bone marrow examination

 • Cell types

 • Cellular composition of bone marrow

 • Morphology of the cells

 • Interpretation of bone marrow examination

Objective
After performing this experiment you will be able to:
Perform the cut out test for the given sample of food product.
Principle

The cut-out test is conducted to evaluate the overall quality of canned food. This test involves examining the condition of the food contents, the external and internal state of the can, and other characteristics of the product through various organoleptic, physical, and chemical assessments.

Materials and Equipment

1. Canned food: 4?6 nos. Cans

2. Tone tester

3. Physical balance

4. Vacuum gauge

5. Can opener

6. Brix refractometer

7. Scale

8. pH paper near neutral ranges.

• General considerations

 • Indications

 • Collection of cerebrospinal fluid

 • Sub-occipital puncture

 • Lumbar puncture

 • Examination

 • Physical examination

 • Chemical examination

 • Cytological examination

 • Microbiological examination

Eyes Anatomy The eye has following important structures. •     Choroid is the layer containing blood vessels that lines the back of the eye and is located between the retina and the sclera. •     Ciliary body is the structure containing muscle and is located behind the iris (that assist in focusing the lens). •     Cornea is the clear outer layer at the front of the eye that transmits and focuses (i.e., sharpness or clarity) light into the eye. •     Fovea is the depression in the inner retinal surface, the photoreceptor layer of which is entirely cones specialized for maximum visual acuity (sharp vision). •     Iris is the colored part of the eye regulating the amount of light entering the eye. In bright light,

Examination of the eyes and ears is an important component of complete clinical assessment. This chapter describes practical evaluation of ocular structures and ear abnormalities in veterinary patients. Eye examination includes assessment of the eyelids, conjunctiva, cornea, pupils and visual responses. Tests such as menace response, palpebral reflex, pupillary light response, Schirmer tear test and fluorescein staining help evaluate ocular function and disease. Ear examination includes inspection of the pinna and otoscopic examination of the ear canal. These procedures assist in identifying inflammatory, infectious, traumatic and neurological abnormalities and provide important information for diagnosis and appropriate treatment.

• General considerations

 • Collection of feces

 • Storage

 • Examination of feces

 • Gross examination

 • Microscopic examination

 • Examination of feces for flukes

 • Examination of feces for blood

 • Culture of feces for nematode larvae

 • Sporulation of coccidia.

• General considerations

 • Collection of milk sample

 • Storage

 • Examination of milk

 • Physical examination

 • Chemical examination

 • Cytological examination

 • Bacteriological examination

Examination of peritoneal fluid provides valuable information about abdominal and systemic disease. This chapter describes the practical collection and evaluation of peritoneal fluid, including appropriate sampling techniques and clinical considerations. Fluid examination helps differentiate transudates, modified transudates and exudates and can provide evidence of inflammation, infection, haemorrhage or other abdominal abnormalities. Physical, biochemical and cytological characteristics contribute to interpretation. Proper collection and handling of samples are essential for reliable diagnostic results. The chapter emphasizes integration of peritoneal fluid findings with history, physical examination and other diagnostic procedures to support identification of abdominal disease processes.

• General considerations

 • Volume

 • Colour

 • Reaction

 • Concentration of spermatozoa

 • Motility

 • Live and dead count

 • Microbiological examination

Deviation from normal well being may be for various kinds involving one or more organs and exhibiting array of symptoms. Examination of animals for diagnosis of disease is strenuous process because investigator has just got to know by several means of examination. The evidences produced by owner, animal house, feed being provided supported by prevailing environment and climate are immensely helpful in accurate diagnosis. Diagnosis, of production related diseases, is more difficult because of biasness in history revealed by the owner. At the same time diagnoses of poisonings are also not easy because owner’s description about possible cause and symptoms exhibited by sick animals are vague. So careful quizzing of owner about each and every aspect of disease and animal obviously seems to be first step towards diagnosis, followed by examination of animal. Examination of environment in relation to history revealed by owner and observations drawn from the examination of animal can infer some conclusion about cause of disease. Support of laboratory examinations such as fecal test, blood test and urinalysis are important tools in disease investigation. Following steps are essential in disease diagnosis.

The essential database for all skin diseases consists skin scrapings. Skin scrapings fall into two main categories: superficial and deep. Superficial scrapings give information from the epidermis’s surface without causing capillary bleeding. Capillary haemorrhage is a sign that the sampling was deep enough to gather material from the hair follicle. Deep skin scrapings are used for this purpose. The main purpose of skin scrapings is to detect the presence or absence of mites. A skin-scraping spatula, which is a thin metal weighing spatula, is recommended for use while scraping the skin. These spatulas are safe and can be used again. Scrapings from active lesions at several places are to be submitted. Scraping from active lesions at several places is to be submitted. Generally, the periphery of the lesion contains more parasites than the centre. Skin scrapings are used to identify ectoparasites and fungal diseases.

• General considerations

 • Examination of skin scrapings for mange

 • Examination of skin scrapings for fungi

 • Cultural examination

General Considerations

The skin scrapings are examined for the diagnosis of skin diseases, including parasitic, fungal and bacterial infections and infestations.