eChapters

Millets are highly nutritious and play a significant role in many people’s diets around the world. They have traditionally been transformed into a range of products through fermentation and malting for centuries. In the era of sustainable development, we still lag in using millets as a primary source of nutrition even after knowing that they are rich in terms of nutrition and other essential elements as compared to cereals. Today we have lots of information regarding different millets; nutrition content, methods of preservation, fermentation, malting, its products etc. Foods made from millet are regarded as potential prebiotics because they can improve the availability or functionality of probiotics, which has major positive effects on health. Foods have been fermented in order to enhance their flavor, texture and palatability. Although fermentation can result in a variety of products, the most prevalent ones are ethanol, lactic acid, carbon dioxide and hydrogen gas (H2 ). Commercial applications for these materials include use in food, vitamins, medications and industrial chemicals. Additionally, fermentation increases the amount of protein present, improves the accessibility of carbohydrates and reduces antinutritional components like tannin and phytic acid. Even though it has some drawbacks, such as the potential for contamination with dangerous microbes, fermentation technology is proven to enhance the nutritional quality of food products. This chapter covers the millet fermentation technology’s effects on the nutritional composition of the millets, contemporary developments in fermented millet-based products and technological improvements examined in the millet fermentation technology.

INTRODUCTION

For thousands of years fermentation processes were largely natural phenomena exclusively identified with food preservation throughout the world. Fermentations can play a significant role in meeting the ever growing food requirements of world population providing benefits in the form of innovative food products with improved nutritional value and food quality along side better safety. Fermentations can be used to address a large variety of food related problems and in the development of processes to meet the demand for food. In the wake of estimations made by international organizations that global food demand and population would increase by two-fold by the year 2050, much emphasis has been placed over the ways and means to increase food production and preservation.

Introduction

Wine is a very unique fermented beverage. It was an essential part of various religious and cultural ceremonies in ancient civilizations; and continues to be considered as a ‘drink for elite’. The reason is that wine was considered as ‘Gift of God and ‘God’s nectar’ in religious text books, and medicinal value of the beverage is well supported by modern science. Wine is a beverage well tagged with its geographical origin. This concept originally comes from French concept of terroir; which explains that a varietal wine from a defined region possess distinctive qualities. Wine makers of the past enjoyed this definition of quality for better marketing opportunities. Today, globalization and advancement in wine science created empowered consumers who could clearly discriminate quality of wines. In addition to a product with high sensory qualities, consumers expect a healthful beverage produced in an environmentally sustainable manner. Enology research projects focus on such aspects; and this chapter is a glance on enology advancements and proven health benefits of wine, such as cardiovascular health gut health, dental health etc.

Introduction

Fermented foods are those foods which have been subjected to the action of microorganisms or enzymes that cause desirable biochemical changes and significant modification of the food (Campbell-Piatt, 1994). Fermented foods are usually produced using ingredients of plants or animals in combination with bacteria or fungi. The bacteria or fungi are either sourced from cultures maintained by humans or from the environment. The origins of fermented foods go back many thousands of years, with early evidence of the alcoholic fermentations of barley to beer and grapes to wine. Evidence suggests that fermented foods were consumed 7,000 years ago in Babylon (Battcock and Azam-Ali, 1998).Throughout the world, the fermented foods are used by the people. It plays a major role in the diet in many developing countries. Several traditional fermented foods are prepared in rural areas of many countries.

Fermentation in food processing typically is the conversion of carbohydrates to alcohols and carbon dioxide or organic acids using yeasts, bacteria, or a combination thereof, under anaerobic conditions.

2.1. The Story of Fermentation

Human progress depends on man’s tact to tame the forces of nature. It calls for knowledge. Prior to Age of Science, our fund of such knowledge was gained by a process of trial, error and accidental discovery. Progress was due not so much to man’s understanding as it was to his unquenchable spirit, miraculous ingenuity and indomitable persistence.

At last, in the utmost urgency of struggle for a longer, healthier and more satisfying existence, humanity taught itself the scientific method, which has changed the world, and will change it more in the coming years. This chapter deals with one of the most momentous and far reaching of all scientific achievements: the ability to harness microscopic living creatures in the service of mankind. Thanks to the wonders of fermentation technology and biochemical engineering developed by scientists and skilled technicians in industrial, governmental and university laboratories around the world, billions of microbes are now at work in industry, producing substances that give us better food $#x0026; drink, better clothing $#x0026; shelter and better medicines. Thus, the human race has entered a new era of health, comfort and longevity.

In the Beginning Art without Science: The cave man discovered that meat allowed to stand a few days was more pleasing to the taste than meat eaten soon after the kill. The cave man’s earliest descendants learned that intoxicating drinks could be made from grains and fruits.

Introduction

Fermentation is an alluring procedure of biochemical adjustment of essential nourishment microorganisms and their enzymes accomplish by lattice. Fermentation is a utilized to upgrade bio-accessibility and bioavailability of supplements from various crops and improves organoleptic properties (e.g., appearance, generation of sour taste and new aroma compounds, and textural changes such as consistency or viscosity) just as increase the shelf life of realistic usability. It makes foodstuff safe not just hindering the growth of pathogenic microorganisms because of antimicrobial growth of lactic acid and it also detoxifies aflatoxin. With these advantages, fermentation is a successful method to reduce the risk of mineral deficiency among population, particularly in developing nations where unrefined cereal or pulses are profoundly consumed. Tragically, it is additionally connected with multiplication of microorganisms, for ex., yeast and molds caused food safety concerns, (Omemu et al; 2011), decrease in provitamin A and antioxidant (Ortiz et al; 2017), carotenoids, just as loss of nutrients and minerals (Hotz & Gibson, 2007).

Fermentation is a minimum cost-efficient procedure for production and preservation of nutrient, therefore fermented food is an important piece of the eating regimen in numerous of the world. In the nineteenth century the scientific study of microbiology began, and just because, the scientific procedure of fermentation was comprehended. several classifications have been utilized to sort the assorted exhibit of the fermented nourishment produced dependent on various microorganisms, and food types, and kinds of fermentation included.

The microorganisms associated with fermentation process fall into three classes: bacteria, yeast, and molds. Yeasts are the principal microorganisms for the fermentation of breads, whereas molds is mainly used in processing of cheese and legumes. Bacteria is included only of the fermentation of cereal product. cereal and tuber fermentation are a lactic and acetic acid producing bacteria. yeast is a piece of mix microbial fermentation by bacteria in weaning food; all the more frequently, be that as it may, alcoholic fermentation of yeast may not totally alluring. Four genera of lactic acid producing bacteria are recognized as overwhelming cereal fermentation to be specific, Lactobacillus, Leuconostoc, Pediococcus, and Lactococcus spp. all of which require carbohydrate for the energy. Lactic acid and other natural acids produced from hexoses bring down the pH from about 6.5 to about 3.6. In the event that the pH is beneath 4.0, fermented product retard the growth of pathogens and along these lines ensure fermented items.

Fermented foods are food substrates that are produced due to the cumulative activity, usually of edible microorganisms, whose enzymes, particularly amylases, proteases and lipases hydrolyze the polysaccharides, proteins and lipids to non-toxic products with flavours, aromas and textures pleasant and attractive to the human consumer. The role played by fermentation during food processing helps in – enrichment of human dietary through development of wide diversity of flavours, aromas and textures in food; preservation of substantial amounts of food through lactic acid, alcoholic, acetic acid, alkaline fermentation and high salt fermentations; enrichment of food substrates biologically with vitamins, protein, essential amino acids, essential fatty acids and antibiotics; detoxification during food fermentation processing and reducing the cooking times and fuel requirements.

Fermentation plays a major role in our diet and give the basic foods like bread, yoghurt, wine and cereal and legume based traditional fermented foods. The development of fermented food products can be considered as one of the greatest achievements of human civilization and goes back thousands of years. It arose from the necessity to conserve foods, to make them more digestible and also more enjoyable. During the centuries, man learned by trial and error to conduct the fermentation processes by changing physical and chemical parameters in the basic food ingredients. It took much longer, however, to discover the cause of fermentation processes: only towards the end of the 19th century it was demonstrated, that baker’s yeast is the cause of alcoholic fermentation. Through the development of the technique of "pure microbial culture", the basis for a controlled use of microbes in food industry was established.

The unique feature of fermented foods is the vital role of microorganisms that bring about essential bio-trasformations of the substrates during fermentation contributing number of desirable properties such as improved product shelf life, enriched diet with improved flavor and texture, increased safety, enriched nutritional supplement and probiotic functions. Fermentation is a chemical reaction carried out by many types of microorganisms to obtain energy. Fermentation is a process for production of a product by means of mass culture of microorganisms. In fermentation, microorganisms breakdown complex organic compounds into simpler substances. Although chemical changes and microbial growth usually mean food spoilage, in some cases fermentation is desirable and microorganisms are actually added to foods. For example, in the production of beer, wine, and other alcoholic beverages, yeasts convert sugar into ethyl alcohol and carbon dioxide. In the making of yogurt and cheese, lactic acid bacteria convert lactose, a sugar found in milk, to lactic acid. Alcohol, acids, and other compounds produced in fermentation act as preservatives, inhibiting further microbial growth. In addition to its use with alcoholic beverages, cheese, and yoghurt, fermentation is used to produce yeast bread, soy sauce, cucumber pickles, sauerkraut, and other traditional fermented food products. The variation in agroclimatic conditions prevailing in India and diverse form of dietary culture of various ethnical groups produced traditional fermented foods such as idli, dosa (rice-legume based), vada, papad, wari, balle (legume based), bhatura, jelabi, nan (wheat based), paneer, shrikhand (milk based).

Introduction

Eradication of malnutrition and hunger (Sustainable Development Goal, SDG-2) are the major challenges to humanity. Availability of sufficient nutritionally balanced food is the prerequisite for ensuring global food security. Food security is also critical for achievement of SDG 1 (poverty eradication) and SDG 3 (health and well-being) (UNDP, 2019). Changing climatic conditions accompanied by global warming not only acts a major threat for achievement of SDGs but could actually lead to the reversal of the progress already made. The escalating global temperature has jeopardized the food security throughout the world. A great decrease in the global crop yield has been suspected even with a 2°C rise in temperature. On field, it is often associated with crop nutrient loss, less accumulation of micronutrients and low availability of micro-minerals like iron and zinc. Additionally, the effect of the rainfall and temperature has also been observed. The global food prices are projected to increase by 3–84% by 2050 due to the fluctuations in rainfall and temperature (Hoegh-Guldberg et al., 2018).

Millets are the small seeded grains which belong to family Grassacae/Poaceae. Traditionally, millets are used for the food and feed purpose and are among the oldest cultivated crops. The millet species of commercial importance are proso (Panicum miliaceum), foxtail (Setaria italica), barnyard (Echinochloa esculenta), finger (Eleusine coracana), little (Panicum sumatrense) and pearl (Pennisetum glaucum) in different regions of the world. In India, foxtail millet (priyangava), barnyard millet (aanava) and black finger millet (shyaamaka) have been mentioned even in the Yajurveda texts of 4500 BC (Morya et al., 2017). The records of consumption of foxtail and proso millet are as old as 2000 to 1000 BC in China. Cultivation of foxtail millet in Korean peninsula is documented around 3,500–2,000 BC. Foxtail millet is the oldest of all the cultivated millets and is believed to be originated in Southern Asia. From there, it was spread to Europian nations. In the west, cultivation of millets majorly started after 1850 (ICRISAT, 2019)

Introduction

Fermentation of food dates back to ancient practice of wine making. Fermented foods reflect culture and tradition of folklore and emphasis their close association with nature. Various methods employed for fermenting foods aimed in food preservation to reduce wastage; however lactic acid fermentation enhanced nutrition content and improved organoleptic properties. Experimental evidences on benefits on health and wellness on consumption of fermented foods are being reported. Fermentation process used for preparation of indigenous or traditional foods often depend entirely on natural microflora on the surface of raw material and containers used; Practices of fermenting foods are passed on as traditional know how to generations as a legacy. The fermented food manufacturers rely on commercial starter cultures, and highly reliant on indigenous materials as microbial collections are unique and highly variable according to region and products. Therefore, isolation of novel organisms, development of newer products and interactions are being explored using variety of fermented foods obtained through traditional processes

Introduction

The art of fermentation process is developed as far back as thousand years ago without understanding the role of microorganism in it. It is believed that the process of fermentation originates in Indus valley during 7000 – 8000 BC. Archives of harappan civilization showed that different clay utensils were used for the fermented product and the agriculture and animal husbandry were fully developed during that period. It is evident from the artifacts of Egypt and middle east that the practice of fermentation was common in that part (Farnworth, 2003). The earliest practice of cheese making is originated from Falcate between Tigris and the Euphrates rivers in Iraq, 8000 years back. Later, Egyptians developed the use of yeast in wine and bread making during 4000 BC (Ross et al., 2002). Further, Sumarians prepared beer from fermented barley during 1750 BC. Earlier the fermenting process was an artisan way without any knowledge of microorganism. However, the main turning point was in 1608, when Antony Van Leeuwenhoek first established the use of a microscope and revealed the existence of microorganisms. Another, major finding was Pasteurization by Louis Pasteur in 1861 AD and this was the first report that documented the role of microorganism in fermentation. Later, there was a shift observed in the production of fermented food from local communities to large scale industry. The health effect of fermented food was first reported by Roman historian Pliny, who reported that fermented milk is beneficial for gastrointestinal complains. Further, a French paediatrician proved that Bifidobacteria in food prevents infants against infection (Grill et al., 1995).

1. Introduction

Now a days, foods are not intended to only satisfy hunger and to provide necessary nutrients but also to prevent nutrition-related diseases and improve physical and mental well-being of the consumers (Menrad, 2003). In this regard, functional foods play an important role. The concept of functional foods which has evolved as the role of food in the maintenance of health and well-being and in the prevention of disease, has received increased scientific and commercial interest is recent past. Growth of functional foods markets include increased consumer health awareness, increased popularity of healthy foods, search for food-based drug substitutes, self-medication approach promoted by busy lifestyles and rapidly developing health conscious markets. Functional foods can be defined as foods “that can beneficially affect one or more target functions in the body, beyond adequate nutritional effect, in a way relevant to an improved state of health and wellbeing and/or reduction of risk of disease” (Stanton et al., 2005).

14.1 Introduction
India is now the top producer of milk globally, accounting for 13.1% of the world’s total milk output. Fresh milk, if left at room temperature, will spoil within a few hours. To extend its shelf life, some of the fresh milk can be converted into fermented products. This fermentation process not only prolongs the product’s shelf life but also enhances its flavor and makes it easier to digest. Historical evidence suggests that people have been making fermented milk products since as far back as 10,000 B.C. Various strains of Lactobacilli have been cultivated in labs to create a diverse array of cultured milk products with unique flavors. Since as early as 10,000 B.C., milk has been utilized to produce fermented dairy items in different parts of the world. These fermented products offer benefits such as improved digestibility, distinctive tastes, added probiotics, vitamins, minerals, and extended shelf life. There is an enormous diversity of fermented dairy products globally, with each product’s characteristics influenced by the local strains used in the fermentation process.
14.2 Lassi
In India and Pakistan, lassi is a somewhat common beverage. It is produced by combining Indian spices with water or milk and curd or yogurt. Sweet lassi is made with sugar or fruits rather than spices; traditional lassi, sometimes called salted lassi, or just lassi, is a savory beverage occasionally seasoned with ground roasted cumin.

Pteridophytes such as ferns belonging to several families, selaginellas popularly called ‘club mosses’ belonging to Selaginellaceae and the Lycopods which resemble both these, belonging to Lycopodiaceae are shade loving plants adapted to cool and moist or hot and humid ecosystems of tropical to temperate climates. They are mostly epiphytic or lithophytic and are grown as foliage ornamentals in a fernery or in shaded areas in gardens. Many are favoured indoor plants.

Popularly grown ferns, lycopods and selaginellas of gardens are listed below:

Adiantum capillus-veneris

Adiantaceae

Common Maidenhair fern

Plants are shade loving bushy lithophytes and have delicate looking thin black stems carrying light green oblique-ovate fronds. They are pot grown as ornamental foliage plants.

Adiantum decorum

Syn. Adiantum raddianum

Adiantaceae

Maidenhair fern

1. Ornamental ferns

Adiantum capillus – Maiden hair fern

Asplenium nidus – Bird’s nest fern

Cheilanthes argentea – Silver fern

Davallia bullata – Squirrels foot fern

Nephrolepsis aureum - Boston fern

Polypodium aureum – Hare’s foot fern

Pteris cretica – Brake/ Ribbon fern

Drynaria quercifolia -Oak-leaf fern / basket fern

Definition: Ferns are flowerless plant that has feathery or leafy fronds. These are reproduced by spores released from the undersides of the fronds.

It is a member of a group of about 10,560 known extant that differ from mosses by being vascular (i.e. having water-conducting vessels). Like other vascular plants, they have branched stems and leaves.

A wide range of beautiful plants exist among ferns which are very popular worldwide. Ferns are grown for their beautiful foliage and their gracefulness. Most of the house ferns are native of tropical regions and are ideal for indoor planting, as they can grow well under shaded or semi-shaded situations. Many species of ferns are native to India. Some of them can be easily grown while others are much difficult, but can be grown well preferably under suitable growing media and proper management.

Fig 8.1: A scheme for fertigation program working with automation

In modern farming with fertigation machines (NutriCare) and automated drip irrigation systems, fertigation set up schemes like the above are put into practice. This scheme will stream line the installation, operation and monitoring of the entire process. An informed grower would be able to control crop performance more precisely and achieve his objectives.

The crop growing environment, both soil/media and aerial are controlled in green house. Therefore, the movement of water (liquid and vapour) and minerals (nutrient ions) follow a different pattern altogether. This factor should be considered while preparing the fertigation program. This becomes all the more different if soil-less rooting media is used.

Table 7.1: Differences in Nutrient uptake in open field and Protected (GH) conditions.

A. CEREALS

1. Rice (Oryza sativa)

RD 60:20:20 kg/acre NPK

(Recommended by TNAU)