eChapters

21.1 Liquid Nitrogen Container/ Cryocan

To store liquid nitrogen a special type of container is used and is known as a LIQUID NITROGEN CONTAI NER OR CRYOCAN. Frozen semen doses are always preserved in liquid nitrogen which is an inert liquid having a boiling point of -1960C and a freezing point of -212°C. Liquid Nitrogen Containers are doubled walled metal (Stainless Steel/Aluminium) vacuum vessels with a very efficient insulation system. Due to highly efficient insulation system the temperature inside the container can remain -196 oC as long as atleast two inches of Liquid Nitrogen is present in the container. It has got an inner chamber which is suspended in outer chamber through neck tube which is a non metal and bad conductor of heat. Walls of the inner chamber are coated with a high quality of insulation material. The space between the inner and outer chamber is also filled with high quality insulation material. Vacuum is created in between inner and outer chamber. Loss of vacuum will lead to the boiling of the liquid nitrogen gas and hence rapid loss of this gas from the inner chamber.

1. UNIT OF CERTIFICATION

The entire area planted under seed production programme by an individual constitutes an unit, provided;

1. All the seed production programme is to produce seed of one category and one variety.
2. Area does not exceed 10 hectares and scattered fields constituting one unit (10 ha) should not be separated by more than 50 metres distance (Table 1).
3. The crop of the entire area is more or less of the same growth stage. The seed crop is grown as sole crop and it is not heavily and uniformly lodged.

I. FILL UPS

1. _____ is the observation of number of plants / earheads of seed crop which should be observed during field inspection as one unit depending upon cropping pattern and crop.
2. Number of plants / earheads to be observed in one count for paddy is _____.
3. Number of plants / earheads to be observed in one count for sesame is _____.

I. FILL UPS

1. Lab lab is a _____ crop.
2. Spacing adopted for lablab seed production is _____.
3. The isolation distance for lab lab crop is _____.
4. The offtypes permitted for CS in lab lab crop is _____.
5. Seed rate for field lab lab is _____.

Introduction

For optimum use of harvested rain water in surface reservoir like farm ponds or tankas, small sized solar PV pumping systems with 1 hp AC and DC motor were experimentally tested at research farms of ICAR-Central Arid Zone Research Institute, Jodhpur (Fig. 16.1). Total suction head in both pumps was about 5 m. Among two installed pumps, the system with AC pump consisted of 1400 Wp (200 Wp × 7) whereas the DC pump consisted of PV array of 920 Wp (230Wp × 4). Each PV panel of AC solar pump was connected in series, which was further connected with an inverter to generate AC output of about 220-240 volt and 4-4.2 ampere. In case of DC solar pump, panels are connected in parallel to generate DC output of 40-50 volt and 15-20 ampere.

Plant breeding activities basically involve development of new and superior crop varieties to replace the old ones and this naturally needs comparison of several lines/cultivars for agronomically important traits which are often quantitative in nature. Therefore, lots of environmental influences are there on the mean performance and mere numerical comparison of mean data is not sufficient to draw valid conclusions regarding the superiority of one variety over the other. Great advances have been made during the last about 100 years in the area of experimental designs and analytical procedures to facilitate such comparisons. Most commonly used designs by the plant breeders are included here for better understanding by the students and the breeders and to handle the experimental data on their own when confronted with such issues. Before applying statistical procedures on the experimental designs, it must be well understood that proper use of field plot technique is of great value to have meaningful data for further statistical analysis and use.

Introduction

Agricultural production in India is constrained by number of biotic and abiotic factors. India is a tropical country and has a climate which is conducive for insect pests which are, one of the major constraints in agricultural production, processing and storage. Annual crop losses due to insect pests and diseases in India are estimated to be 18 percent of the agricultural output. Insect pests are widespread in all the agroclimatic zones and large quantities of insecticides are used to control these pests. Indiscriminate use of insecticides, lack of adequate knowledge on proper application techniques and dosages, and frequent use of broad spectrum insecticides are common in India. There is a need to identify suitable alternative methods for the management of insect pests and among them, biological control using viruses, bacteria, fungi and entomopathogenic nematodes is gaining greater attention world over.

Nematode families of Steinernematidae and Heterorhabditidae attracted most attention as they contain the entomopathogenic nematodes (EPN). Entomopathogenic nematodes are obligate parasites of insects that kill their hosts with the aid of bacteria (Xenorhabdus and Photorhabdus) carried in the nematode’s alimentary canal. The lethal obligate insect parasites are ubiquitously distributed without pathogenicity to mammals and beneficial non-target insects, and exempted from registration requirements. Currently there are about 98 valid EPN species belonging to Steinernema (79) and Heterorhabditis (19). Soil surveys conducted in divergent areas of Indian subcontinent have demonstrated high diversity of EPN across seasons, habitats, and geographic regions. There are several target pests in India that can be controlled with entomopathogenic nematodes and several workers have used EPN against cutworms, stem borer, white grubs, etc. under laboratory and field conditions and are compatible with existing IPM programmes.

1. INTRODUCTION

Vegetables differ widely from field crops in their requirements for land preparation and cultural practices because of their differences in their morphologies, growth habit, and socio-economic values. In preparing land for vegetable production, ecological location, mode of cropping, season, crop disposition and the type of vegetables to be grown are taken into consideration.

Clearing: It is often necessary to remove the vegetation cover when a piece of land is to be used for vegetable production. The land clearing methods may involve removing the remains of previous crops and undergrowth, cutting back the woody shrubs and trees and burning them.

Levelling: When the site has been cleared, uneven land may have to be leveled. This facilitates the process of ploughing, harrowing ridging and layout of the site.

Land preparation and layout depend on

Minimum field standards for seed production have been prescribed  for each crop. The major components to be looked into for meeting the field standards are: 
   Preceding crops 
   Isolation distance 
   Number of field inspections 

The minimum proceding crop requirement has been specified to minimize genetic contamination on account of volunteer plants germinating from the left over seeds in the previous season. The disease contamination is also possible from soil borne disease organisms if the previous crop was not certified. The preceding crop requirement would mean that for a prescribed minimum period the same crop or any other contaminating crop should not have been grown on the same field where seed production is to be taken up unless the preceding crop was a seed crop and had met the field/the seed standards.

Many farmer participatory field  experiments were designed and conducted in different states of India having large area under acid soils, with locally available soil ameliorants to study their effect on soil health and crop performance. This work was done during 2000 & 2005 under an ICAR network project on “Soil characterization and resource management of acid soil regions for increasing productivity”. The details of the field locations are presented in table 1.

13.1 Biosafety Research Trials: Scope and Implementation
Introduction
Biosafety research trials serve as a critical phase in the evaluation and regulation of genetically modified organisms (GMOs), particularly transgenic crops and microorganisms. These trials assess potential environmental and health impacts under confined and controlled conditions before the organism can be considered for commercial release. With the expansion of biotechnology applications, especially in agriculture, pharmaceuticals, and industrial processes, the need for scientifically rigorous and ethically sound biosafety research trials has become increasingly important.
13.1.1. Scope of Biosafety Research Trials
1.1. Definition and Objectives
Biosafety research trials are confined, stepwise, and monitored field or laboratory-based studies designed to evaluate the safety, stability, performance, and unintended effects of GMOs. The overarching objectives include:
• Assessment of potential risks to human health, non-target organisms, and the environment.
• Evaluation of gene stability, trait expression, and environmental adaptability.
• Monitoring of gene flow and potential invasiveness or weediness.
• Determination of agronomic efficacy under field conditions.
• Data generation for regulatory dossiers and risk assessment reports.

A field visit to innovative farming system models would involve observing and learning about different approaches to agriculture that aim to improve efficiency, sustainability, and profitability. Here’s a breakdown of what such a visit might entail, the types of systems you might encounter, and the potential benefits:
Objectives of a Field Visit
• Practical Learning: See firsthand how innovative systems are implemented and operated.
• Knowledge Exchange: Interact with farmers, researchers, and experts who are using these systems.
• Inspiration & Idea Generation: Identify potential adaptations and applications for your own farm or region.
• Networking: Connect with other individuals and organizations interested in sustainable agriculture.
• Assessment: Evaluate the real-world performance of different technologies and practices.

The Aquatic Resources Research Institute (ARRI) was established to conduct research & answer important impending questions associated with the nation’s aquatic resources, like conservation of marine organisms, marine science, oceanography, aquaculture, biotechnology etc. It also works for transferring or sharing the knowledge to public, for guiding towards sustainable development.

Sichang Marine Science Research & Training Station: (SMaRT) Chonburi Province

Thailand This is another unit of the ARRI. It was established in 1979, in Chonburi Province Thailand.

Fieldpea is animportantrabi pulse crop which is highly productive and is grown for food, feed as a vegetable. There are two types of peas grown in India viz., vegetable type in which grains arevery sweet when green and become wrinkled ondrying, and grain type where grains are generally white and round. Green seeds of the grain type are not so sweet as in thecase ofvegetable type. The dry grains areconsumed in various forms such as chat, chhola, dal, vegetable and flour. Green seeds are used as fresh, frozen or canned vegetables. The cultivation of grain type is confined to northern and central parts of the country.

It is high protein (20%) crop with all the essential aminoacids important for normal activity of living organisms. The fractional composition is also favourable the most easily assimilated water soluble protein from mature pea seeds varies from 36 to 87% and in green matter from 65 to 82%. Pea contains 4 mgpro-vitamin A, 300 mgvitamin C, 3 mgB₁, 1.5 mgB₂and 1.2 mgpantothenic acid per 1000 g fresh seed weight. Also, it contains 1.1% fat, 2.2% minerals, 4.5% fibre and 56.5% carbohydrate. It is rich in phosphorus. Green pea and the immature pods of pea are typified by a high level of active lipotropic antisclerotic substances-choline and inositol.Choline deficiency may lead to the development and growth of malignant tumors.

Ficus carica L, one of the oldest plant species in China, is abound in Shandong, Xinjiang, Jiangsu province. Fig belongs to the family Moraceae and genus Ficus. It is native to Middle East region (West Asia), but now it is widely distributed in Asia and North America.

Cultivars/Varieties: Depending upon the nature of flowers and the method of pollination there are four distinct classes of fig- i) Common fig or Adriatic fig ii) Capri fig, iii) Smyrna fig and iv) San Pedro fig. Kadota, Mission, Adriatic, Brown turkey, Celeste and Conadria are some important varieties of common fig.

Uses

Fig is consumed fresh or dried, preserved, candied or canned. Fresh figs are very delicious, wholesoms and nutritious and used as dessert or for making jam. Due to its highly perishable nature it is dried and marketed. The fruit fresh, or dried is valued for its laxative property. Dried and ground fig can be used as a substitute for coffee.

Fig (Ficus carica  Linn.)  also called ‘Anjeer’ is a nutritive and delicious fruit. Its tree is native to western Asia. It is rich in calories (269), protein, and calcium (higher than milk), iron and fibre content. The small deciduous plants of fig are grown in different parts of the country. Fig has been cultivated since ancient period (4000 BC.) and well known through out the world. It is originated in Middle East and naturalized in many parts of the world. Fig tree plantations cover 426,244 ha. area in the world with an overall yield 1070676million tones. The largest fig growing countries are Turkey, Egypt, Iran, Greece and Algeria (Pasqual and Ferreira, 2007). It is also grown in some parts of Australia, California, New- Zealand and Argentina.

Fig is one of those fruits which are known to mankind since early ages. Legend maintains that the Fig was the “Autumn Fruit’ as revealed by the Greek Goddess, Demeter, and the tree is still held sacred in most parts of the Mediterranean. The fig’s nutritional value is so high that it is often referred to as the ‘Fitness Fruit’. It is a rich source of dietary fiber. It contains a good amount of vitamins and various useful minerals like iron, calcium, magnesium, copper, etc. The matured fruit has a tough peel either pure green, green suffused with brown, brown or purple. The interior is a white containing a seed mass bound with jelly-like flesh.

Genus : Ficus 
Species : carica L.
Family : Moraceae
Chromosome No. : 2n = 26

Fig along with fruits like date palm, grape (vinifera) and olive was regarded as an important food crop of the ancient civilization that developed in the Mediterranean region. The fig description occurred prominently in number of songs as well as in the mythological and historical events that took place at that time. Fig is thought to be native of southern parts of Arabian peninsula, Italy, Balkan and USSR region. Fig was in cultivation as early as 300 BC and from Arabian peninsula it spread to Turkey, Syria, Iraq and later to many subtropical parts of the western hemisphere.

• Poona fig is the most popular cultivar grown in India (5.0 m × 5.0 m)
• Excel and Candia are suitable for HDP (2.5 m × 2.5 m)
• Candia is suitable for drying purpose.
• Excel is suitable for canning purpose.
• Deanna is suitable for table purpose.
• Fig is one of the first fruit to be preserved by drying.
• Salt and drought-resistant crop.
• Ficus carica is gynodioecious spp.
• Caprifig is monoecious spp.
• Common fig is pistillate.
• Excessive irrigation or heavy rains during ripening result in fruit cracking and producing of insipid fruits.
• Cultivar – Merselies, Black Ischia, Kabul, Banglara, Lucknow, (Dinkar – an improvement over Daulatabad).