eChapters

A

Acervulus (pl. Acervuli)—A saucer-shaped, spore-producing body of a fungus embedded in host tissue.

Actinomycetes—Filamentous bacteria that produce several antibiotics and give soil its earthy smell.

Active ingredient: In pesticides, the chemical responsible for the desired effect.

Aggressiveness: Virulent forms of pathogen cause differing degrees of symptom severity.

Alternate Host—One of two kinds of plants on which a parasitic fungus (e.g., rust) must develop to complete its life cycle.

Anamorph—Asexual stage of a fungus.

Antagonism: The counteraction between organisms or groups of organisms.

Plants, with the help of photosynthesis, convert simple molecules (like carbon dioxide and water) into more complex molecules (anabolism) through various chemical reactions. Animals synthesize more complex molecules (anabolism) from monomer units through multiple chemical reactions. Anabolism refers to a biosynthesis process involving the sequences of biochemical reactions by which complex molecules are formed in living cells from available monomer units. Anabolic processes include the biosynthesis of complex biomolecules like carbohydrates, proteins, and lipids. The anabolic processes require energy from high-energy bond compounds like ATP.
The anabolic processes related to the biosynthesis of carbohydrates include
(1) Gluconeogenesis and
(2) Glycogenesis
11.1 Gluconeogenesis
Gluconeogenesis is the synthesis of new glucose molecules from noncarbohydrate molecules. The non-carbohydrate molecules that are used for gluconeogenesis are derived from glycolysis, lipolysis, and proteolysis. Examples are pyruvate, lactate, glycerol, and glucogenic amino acids. The liver and kidneys are the preferred organs for the gluconeogenesis process. It also occurs to some extent in the brain. Blood glucose levels are maintained at physiological concentrations through the process of gluconeogenesis in humans and ruminants. The blood glucose drops below normal levels during various conditions like fasting, starvation, pregnancy, lactation, fever, and low carbohydrate diets.

(Method of Froesch and Renold, 1956; Froesch et al., 1957)
Enzymatic with Glucose-oxidase / Peroxidase

Introduction

Glucose oxidase enzyme specific for the oxidation of glucose, was discovered in a fungus preparation by Müller in 1928. This enzyme is present in bacteria and in moulds such as (Pencillium notatum), which promotes the oxidation of glucose (b-ring form) to gluconic acid with the production of an equivalent amount of hydrogen peroxide. Normal values for whole blood glucose oxidase are 60 to 90 mg/100 ml. Results by the enzyme methods are lower than by the best “true  sugar” reduction methods largely because of their specificity, but inhibition of glucose oxidase by uric acid may be a contributory factor. In suspected cases of diabetes, values below 120 mg per 100 ml call for further investigation, for example, by glucose tolerance test. Due to the presence of glycolytic enzymes in red cells, blood must be collected into an antiglycolytic/anti-coagulant preservative, if there is to be a delay of more than 1 to 2 h. in processing or estimation. A fluoride/oxalate mixture, containing 2 mg of fluoride per ml of blood, is suitable and the glucose content will then remain unchanged for upto 3 days at 4ºC.

A glucose syrup is defined as ‘A purified and concentrated aqueous solution of nutritive saccharides derived from starch’, and having the following characteristics:

1. Dry matter of not less than 70%.
2. A dextrose equivalent (DE), expressed as d-glucose, of not less than 20% based on dry matter.
3. A sulphated ash content of not more than 1% on a dry basis.

Starch has a DE value of zero, whilst dextrose, the final end product of starch hydrolysis, has a DE value of 100. For the lay person, the term dextrose equivalent or DE can be regarded as an indication of how far the conversion process from starch to dextrose has gone. While technically DE is a measure of the total reducing sugars present in a glucose syrup.

Introduction

Wheat is the most important cereal crop to the human kind. It is the main source of energy and nutrition in human diet. Wheat is consumed in the form of large number of end products as chapati, bread, biscuit, cakes, pretzels, noodles and pasta products. Millions of people are involved in milling, baking and pasta products industry with huge turnover per annum. However, each end-use product of wheat has it’s own specific requirement for example basically hard wheats along with strong gluten and high protein content are used for bead making. Soft wheat with weak gluten and low protein content are required for biscuit making Souza et al. (1994). Durum (tetraploid) wheat is used for pasta products as macaroni, vermicelli and spheghatti and Dicoccum for therapeautic purposes. Often chemical improvers are used to improve the dough properties for these products. Most of these chemicals have been banned because of their toxic effects. Therefore, only alternative is to develop varieties suited to meet the requirements of these end-use products. This needs better understanding of molecular basis of grain quality to accelerate breeding for producing wheat varieties for each end-use product separately. Storage proteins are the major component of grain which determines end-product quality. In this chapter detailed structure, genetics and functionality of gluten proteins are described.

Glycogenesis helps to maintain blood glucose, as it is a primary source of energy for every cell. An inadequate supply of glucose to the body may lead to the failure of the vital organs. Glucose, when in excess, is synthesized into glycogen and stored (glycogenesis), mainly in the liver and muscles (including the uterus). It is also found in other tissues, such as the kidney, brain, heart, and adipose tissues. The stored liver glycogen is broken down (glycogenolysis) into blood glucose when needed. Still, the glycogen stored in muscle is broken down to glucose 1-phosphate and converted to blood glucose later. Either the synthesis or breakdown pathway is primarily active at a time in tissues. Glycogen synthesis and its breakdown are not reversible processes.
The percentage of glycogen stored in skeletal muscles is less ( 1% to 2%) than in the liver (10%). Still, the total quantity of glycogen is greater in muscles due to their greater mass than an animal’s total liver mass. Muscle mass, on average, ranges from 40 to 45 % of body weight for the most mature mammals.
Glycogen synthesis (glycogenesis) is an anabolic process forming a branched homopolysaccharide made of several chains of alpha D-glucose units linked by α-1,4 glycosidic bonds in a linear chain and nearly every ten glucose residues; the chain branches off via α-1,6 glycosidic linkages. The α-glycosidic bonds give rise to a helical polymer structure. The number of glucose units in a single glycogen molecule varies (1,700 to -600,000 units). The glycogen granules are formed on the glycogenin protein in the center, so it acts as a primer, and their number determines the total number of glycogen particles.

Glycogen breakdown is known as glycogenolysis. This breakdown occurs in the cytoplasm of cells of the organs, wherein glycogen is stored. The end product of glycolysis is glucose in the liver, which is glucose-1-phosphate in skeletal muscles.
Glycogen is a polysaccharide synthesized from glucose monomers. Glycogen is stored in the cell instead of glucose because glucose requires more water, whereas glycogen can be stored with much less water. Most of the stored glycogen is in the liver (5% of body weight) and muscle (1 to 2 % of body weight). Muscle and liver glycogen undergo glycolysis for different purposes. Muscle glycogen provides energy for muscular activity, whereas liver glycogen helps to maintain the blood glucose level.
Glycogen contains many glucose residues (up to 120,000) linked by alpha 1-4 glycosidic bonds, forming a straight chain. Glycogen has branches at alpha 1-6 glycosidic bonds at every ten glucose residues. At the reducing end, the carbon one of glucose is free, whereas it is not accessible in the case of non-reducing ends.

Glycolysis involves a series of reactions to extract energy from glucose to
release two molecules with three carbons called pyruvate. The process is not
dependent on oxygen but can occur under aerobic and anaerobic conditions,
releasing different end products. Under aerobic conditions, the pyruvate formed
is then oxidized in the mitochondria to CO2 and H2O. The energy released in
glycolysis is in the form of ATP (High Energy Bond). All the reaction steps
occur in the cytoplasm, as it is the extramitochondrial pathway. In anaerobic
conditions and the absence of mitochondria (RBC), the end product of
glycolysis is lactic acid.
The net reaction of Glycolysis (anaerobic) may be summarized as under
Glucose + 2ADP + 2Pi → 2 Lactate + 2ATP
7.1 Steps in EMP Cycle or Glycolytic Pathway
Table 7.1: Steps and enzymes of glycolysis or EMP cycle.

Glycosides are compounds containing a carbohydrate and a non carbohydrate residue in the same molecule. They are the complex polysaccharides which are chemically heterogeneous collection of substances, which have in common one property that the molecules are linked together and contain at least one sugar unit. The term glycoside represents a group of compound containing glucose and one or more basic or acidic organic compound in chemical combination. When the sugar other than glucose is present, it is called glycoside. Each sugar is linked through a glycosidic bond to either a non sugar component, aglycones, (aglycone or aglucone is the nonsugar molecule in glycosides).

Biotech crops or genetically modified (GM) crops or the transgenic crops are those crop plants into which certain genes have been introduced from unrelated sources beyond the boundary of sexual compatibility using techniques which bypass the normal sexual cycle. The transgenic production technology uses recombinant DNA technology where a recombinant DNA molecule is a vector into which the desired DNA fragment has been inserted to enable its cloning in an appropriate host. The recombinant DNA technology has opened up the flood gate of plant biotechnology to produce plants which are equipped with novel genes to produce products never heard of that a plant will do. Now transgenics in several crops have been commercialized globally. The importance and potential of GM crops can be realized from the fact that acreage under such crops at global level has reached to 189.8 million ha covering 24 countries in 2017. One can have a feel of this huge acreage under commercialized GM crops globally if it is compared with about total cultivated area of 143 million ha in India.

Over the last 30 years field of agricultural biotechnology has expanded rapidly due to greater understanding of DNA as the chemical double helix code from which genes are made. Genetic engineering is one of the modern biotechnology tools that are based on recombinant DNA technology. The term genetic engineering, often interchanged with terms such as gene technology, genetic modification, or gene manipulation, is used to describe the process by which the genetic makeup of an organism can be altered using “recombinant DNA technology.” This involves the use of laboratory tools and specific enzymes to cut out, insert, and alter pieces of DNA that contain one or more genes of interest. The ability to manipulate individual genes and to transfer genes between species that would not readily cross is what distinguishes genetic engineering from traditional plant breeding and opens up a huge opportunity to create crop plants equipped with desirable genes from the sources which cannot be sexually crossed. With conventional plant breeding, there is little or no guarantee of obtaining any particular gene combination from the millions of crosses generated. Undesirable genes can be transferred along with desirable genes or while one desirable gene is gained, another is lost because the genes of both parents are mixed together and re-assorted more or less randomly in the offspring. These are the major limitations of conventional crop breeding.

Transgenic varieties combine genes from totally unrelated species. For example, we can now transfer genes for salinity tolerance to rice from mangroves. The recombinant DNA technology is part of the evolution of genetics starting with the rediscovery of Mendel’s Laws of Inheritance in 1900. In the early part of the twentieth century, various techniques like irradiation, use of chemical mutagens and doubling of chromosomes through colchicine treatment were adopted to develop novel genetic combinations.  Today such gene transfer can be done with both precision and ease through recombinant DNA technology. Both molecular marker-assisted breeding and gene transfer now play a very important role in developing genetic combinations to meet the challenges rising from biotic (i.e., pest and diseases) and abiotic (i.e., drought, flood, sea level rise, etc.) stresses. They will gain further importance in the emerging era of climate change. Varieties developed by marker-assisted selection are also eligible for certification under the protocol for products produced through organic farming.

1. GMP

Good manufacturing practices and requirements of premises, plant and equipment for pharmaceutical products

To achieve the objectives listed below, each licensee shall evolve appropriate methodology, systems and procedures which shall be documented and maintained for inspection and reference; and the manufacturing premises shall be used exclusively for production of drugs and no other manufacturing activity shall be undertaken therein.

Introduction

Meat derived from animals are subjected to various contaminations from number of sources. The animal itself is a major contributor of both pathogenic and spoilage organisms. Further contamination may be introduced from water used, equipment used for product preparation, from the meat handlers and the surrounding environment. Microbiological problems such as food poisoning or spoilage may arise when the desired effect is not achieved and is usually due to errors in the handling or processing procedures. The detection and rapid correction of such errors and their future prevention is a major objective of any quality control system.

In traditional approach to meat and meat product quality control system, only the samples of the final product were analyzed by laboratory test to check for compliance with specifications. However, the responsibility for control of hazards rests with individuals involved in all stages from farm to ultimate consumer. And the control of hazards at each stages of food chain may be achieved by Hazard Analysis Critical control point (HACCP). Hazard analysis critical control point is an internationally recognized system that helps to ensure the manufacture of safe food products. HACCP is not a zero risk system but rather a system designated to minimize the risk of food safety hazards. HACCP concept is a systematic and rational approach Firstly by consideration of the hazards i.e., microorganisms or their toxins, that need to be controlled in a specific operation by structured hazard analysis and secondly on the identification of the most effective means of controlling the identified hazards. This system, by focusing attention on the factor that directly affects the microbiological safety and quality of meat foods, eliminates wasteful use of resources and superfluous consideration. As a consequence, the cost/benefit relationships are more favorable. The system is applicable at all levels of meat food chain from farm production through processing, transporting, merchandising and ultimate use in the meat food service establishment or at home. Ensuring food safety and quality is the responsibility of all links in the food chain. Now a day’s food consumers are very much health conscious and they want their food to be safe and of good quality. As meat being highly perishable and high risk food product, it is very much imperative on the part of meat and meat products producers and exporters to produce safe and quality meat to stand in the competitive world and further hygienic meat processing is important not only to provide safe and wholesome meat but to provide a good public image and to increase consumer demand.

31.1 Introduction
A fundamental element of quality assurance is good manufacturing practice (GMP), which ensures that goods are consistently produced and regulated according to the quality standards suitable for their intended application and as outlined by the marketing license. The primary objective of GMP is to mitigate risks in pharmaceutical manufacture, which may be categorised into two main types: erroneous labelling and cross-contamination/mix-ups. Primarily, manufacturers must guarantee that their goods are safe, effective, and of adequate quality; hence, risk assessment has become a crucial component of WHO quality assurance guidelines.
31.2 Implementation of HACCP
31.2.1 HACCP Preamble and Definitions Preamble
1. The introductory portion of this publication delineates the concepts of the Hazard Analysis and Critical Control Point (HACCP) system as endorsed by the Codex Alimentarius Commission. This section provides broad recommendations for system application, recognising that implementation specifics may differ according to the food operation’s unique conditions.
2. The HACCP system, grounded in scientific principles and methodical methodology, identifies specific dangers and implements control measures to assure food safety. It functions as an instrument for hazard evaluation and the implementation of control systems aimed at prevention rather than predominantly on final product testing. The HACCP system is flexible, allowing for modifications in equipment design, processing methods, or technology innovations.
3. HACCP is relevant across the whole food chain, from initial production to ultimate consumption, and its use must be guided by scientific knowledge on dangers to human health. In addition to improving food safety, the use of HACCP can provide other substantial advantages. Furthermore, the use of HACCP systems can streamline regulatory inspections and enhance international trade by increasing confidence in food safety.

Goat anatomy

Understanding of the basic structure and functioning of goats is required to maintain the health and productivity of goats. Anatomy deals with the form and structure of the animal whereas physiology deals with the mechanism of body functions.

The skeleton of goat is made up of the vertebral column, ribs and skull, the limbs, and the joints. There are 7 neck (cervical) vertebrae, 13 chest (thoracic) vertebrae, 6 or 7 lumbar vertebrae, 4 pelvic (sacral) vertebrae and between 4 and 8 tail (coccygeal) vertebrae.The ribs are elongated, curved bones that form the ribcage, which is the skeleton of the thoracic cavity (chest). There are 13 pairs of ribs attached to each side of the thoracic vertebrae. Most of them are fixed i.e. they are joined to the sternum (breastbone) at the front of the chest but the last two pairs are not attached at the front and are called floating ribs.The skull includes all the bones of the head. It consists of a number of flat bones that form immovable joints, most of which disappear with age; and a lower jaw bone (mandible), which forms movable joints with the other parts of the skull.The limbs include forelegs (thoracic limbs) and hindlegs (pelvic limbs). The forelegs consist of shoulder,forearm and a lower limb made up of carpus,metacarpus and phalanges. The hind legs consist of pelvic girdle, thigh, and a lower limb made up of tarsus, metatarsus and phalanges.

The joints are where two or more bones of cartilages meet. There are two main kinds of joints.Fixed or immovable joints have no joint cavity and are united by fibrous tissue or cartilage. Many of these are temporary joints which become fixed as calcium accumulates in the flexible tissues and turns to bone (calcifies) with age. Examples are the joints in theskull, the joints of ribs to vertebrae, and the pelvic and mandibular symphyses (midline joints).Movable joints have a joint cavity surrounded by ajoint capsule. There are two opposing bone surfaces,which have smooth surfaces of very dense bone.

Movable joints have cartilages between the bones to reduce friction and absorb concussion. The inner lining of the joint capsule is the synovial layer, which produces synovial fluid (joint fluid). Examples include joints of the limbs, such as the shoulder and the hip, and the joint between the skull and the vertebral column.

In India, goats are known as “poor man's cow” because they are an important source of income for poor and landless people. They can be reared on cheaper resources easily than other livestock.

Goats come under the genus Capra have evolved from five wild species, including Capra hircus (which includes Bezoar), Capra ibex, Capra caucasica, Capra pyrenaica, and Capra falconeri. This evolutionary pattern contributes to their phenotypic diversity and adaptability to various environments. The chromosome profile of goats (specifically the Capra hircus species) reveals some interesting features which are as follows:

Chromosome Number
The modal chromosome number for goats is 60.
These chromosomes can be categorized into different types based on their centromere position:
Autosomes: These are either acrocentric or telocentric.
X Chromosome: It is acrocentric.
Y Chromosome: It is submetacentric.

Identification

Quinacrine-mustard staining and fluorescence microscopy allow us to identify 28 out of 30 chromosome pairs in goats.

Goat is the 1st domesticated animal in the ancient age and has been considered to be the Poor Man’s Cow Goat’s meat is lean & has low fat and the demand for goat’s meat is increasing, with rising prices. Goats usually give birth to twins & triplets. Goat is a multi functional animal and plays a significant role in the economy and nutrition of landless, small and marginal farmers in the country. Goat rearing is an enterprise which has been practiced by a large section of population in rural areas. It can efficiently survive on available shrubs and trees in adverse harsh environment in low fertility lands where no other crop can be grown. In pastoral and agricultural subsistence societies in India, goats are kept as a source of additional income for their meat, hair fiber, and dairy, or as a pet. Goats are also used in ceremonial feastings and for the payment of social dues. According to FAO (2008) goat milk production in India were 4 million metric tons, Bangladesh 2.16 million metric tons and the Sudan 1.47 million metric tons over much of the world. Goats are among the main meat-producing animals in India, whose meat (chevon) is one of the choicest meats and has huge domestic demand (Shalander Kumar, 2007). Goat rearing is an enterprise which has been practiced by a large section of population in rural areas. Goats can efficiently survive on available shrubs and trees in adverse harsh environment in low fertility lands where no other crop can be grown. (Oberoi et al., 199I) reported that in variable cost, expenditure on labour was the major component of cost and was 81% under goat farming.

Abstract

From the vedic time itself goat is popularly known as a poor man’s cow in India. It has the proven potential to make significant contribution to the poor man’s economy thus livelihood with minimal requirement of care. It can be easily managed by women and children in spare time as it is small-sized animal. Other maintenance requirement like feeding, milking and care of goats do not require much equipment and hard work. Capital investment and feeding costs are also quite low. It not only supplies nutritious and easily digestible milk but goat meat has high demand and price in the market thus can act as regular source of additional income for poor and landless or marginal farmers. Also the role of women in goat keeping is very significant in the poor rural families and goat is the most important means through which rural women are able to contribute meaningfully to the cash needs for their family members. Thus, goat rearing can play very important role in balanced socio-economic development of the country as there is a close relationship between the status of women and the socioeconomic development of any country (Alam, 2001 and Ahmed, 2017).

Gujarat state blessed with rich goat genetic resources. Recognized goat breeds in the state are Kutchi, Surti, Zalawadi, Mehsanba and Gohilwadi which have been the integral component of small, marginal farmers, landless labourers and significantly contribute rural livelihood. These breeds excel in drought capacity, disease resistance and better adapted to harsh local climatic conditions under extensive production system. The population status and breeding tract of goat breeds are given in Table 1.

The goat genetic resources (AnGR) of state may be important assets for having the valuable adaptive traits that they have developed over long periods of time, such as:

Introduction

Uttarakhand has a mix of almost all domesticated species of livestock, very large in number and very low in productivity across the board. In economic terms they are a wealth indeed, low productivity not with standing, as the sector supports livelihoods of nearly 80 percent of the rural households in the state, at least in part in the majority of cases and in full in some (tribal and nomads exclusively living off livestock).

Livestock production in Uttarakhand is the endeavor of the small holder (marginal, small and landless) and takes place in millions of small and tiny holdings scattered across the state. The predominant farming system is mixed crop livestock farming, both in the plains as well as the hills, irrigated in the plains and rainfed in the hills.