eChapters

[Alkaline Permanganate / Subbiah and Asija (1956) Method]

Introduction

Nitrogen (N) is found in the arable horizon of the soil, mostly in organic material. In the soil solution, organic N is gradually transformed into ammoniacal (NH₄ ⁺), nitrite (NO₂ ^–) and nitrate (NO₃ ^–) nitrogen by microbial processes. Organic-N is, in itself, of very little use to plants, as it can not be absorbed as such. It is, therefore, necessary to estimate the different forms of mineralized or available N. The NO₃ ^–_N and NO₂ ^–-N together, hardly, exceed 1 % of the total N in normal soil.

The available N in soil refers to a fraction of the total N which is converted into forms accessible to the plants. This constitutes, on an average, only 0.5-2.5 % (rarely 5 %) of the total N in a soil at any given time. Nitrogen is, generally, absorbed by plants as NO₃ ^–, under oxidized environment (upland condition), and as NH₄ ⁺, under reduced condition. NO₂ ^– is sometimes, detectable, but the amount is, generally, very small vis-a-vis NH₄ ⁺ or NO₃ ^– and, hence does not warrant its determination. The sum total of NH₄ ⁺ and NO₃ ^–- N is smaller than the total N, which becomes available to plants and the difference being attributed to the mineralization of the organic N, during the crop growth cycle.

[Titrimetric/ Walkley and Black (1934) Method]

Introduction

The role of soil organic matter, in relation to soil fertility and physical conditions, are widely recognized. The organic matter is the source of plant nutrients which are released inassimilable forms during microbial degradation. A major proportion of N (95 – 99 % of the total), P (33 – 67 % of the total) and S (75 % of the total) in soils occur in organic combinations, which mineralize to release the nutrients in inorganic forms to be used by plants. Nevertheless, it serves as a reservoir of plant nutrients, in promoting water storage, and in regulating microbial activity.

The organic matter content of a soil varies from 0.344 % in very sandy arid soils to more than 86 % in Peats and Mucks. Soil organic matter contains 5 % N, and 0.5 % each of P and S, thereby, having a N:P:S ratio of 10:1:1. The organic matter content of a soil is estimated by the amount of organic carbon (C) present, as this element represents, on an average, 52 – 58 % of the organic matter, and the C:N ratio is 10 – 15. In the sub-soil, the average organic carbon amount is 36 – 44 % (40 %), and the C:N ratio is < 8. In order to find out the amount of humified organic material, the organic carbon content is multiplied by 1.724, which represents the ratio between the humus and the organic carbon (100/58) and is known as the ‘von Bemmlen factor ’.

The organic matter content of soils is estimated from the organic carbon, determined by using the ‘Titrimetric method / Walkley and Black method. The Titrimetric determination, also known as the ‘wet-digestion method’ involves a rapid titration procedure for the estimation of organic carbon content of soils.

The Electrical Conductivity (EC) of the soil is a measure of soluble salts present in the soil and is expressed as millimhos/cm or dSm^-1.

Principle

As the amount of the soluble salts in a solution increases, the electrical conductivity also increases. This electrical conductivity is measured in terms of resistance offered to the flow of current using a conductivity bridge.

It is known that solutions offer some resistance to the passage of current through them, depending upon the concentration of the soluble salts present. Hence, EC is measured in terms of electrical Resistance between parallel electrodes immersed in the soil water suspension.

In such a system, the solution between the electrodes becomes the electrical conductor to which the physical laws relating to resistance are applicable. The electrical resistance ‘R’ is directly proportional to the distance ‘L’ between the electrodes and inversely proportional to the cross sectional area ‘A’ of the conductor.

The pH is defined as negative logarithm of hydrogen ion concentration or the log of the reciprocal of the hydrogen ion concentration (Sorenson, 1906).

Principle

A glass electrode in contact with hydrogen ions of the solution acquires an electric potential which depends on the concentration of hydrogen ions. This is measured potentiometrically against some reference electrode which is usually a calomel electrode. The potential difference between glass electrode and calomel electrode is expressed in pH units.

Two electrodes are used in the determination of pH. One is reference electrode which provides a standard voltage and is usually a saturated calomel electrode which has two layers of saturated solution of KCl and mixture of solid HgCl₂ and Hg. The outer tube is usually 5-15 cm long, 0.5-1 cm in diameter. The mixture of solid HgCl₂ + Hg paste is contained in an inner tube that is connected to the saturated KCl solution in the outer tube by means of small opening. The resistance developed by this type of electrode is 2000-3000 Ohms.

The outer electrode is glass electrode that consists of a tube enclosing a lead wire made up of Ag coated with AgCl. This wire is again enclosed in a wax insulation. To the tube at the bottom is attached a glass bulb made up of a special kind of glass which is sensitive to hydrogen ions. The thickness of the glass membrane varies from 0.03 to 0.1 mm and has a resistance of 50 to 500 mega Ohms.

When these electrodes are dipped in solution, the saturated solution of KCl comes out of reference through small holes and forms an invisible ionic bridge between electrodes through which current passes. The hydrogen ions are absorbed by glass electrode and depending upon the amount of hydrogen ions present in the solution, an electric potential develops between electrodes. This potential difference is measured in terms of pH units by suitable Galvanometer.

(Keen-Rackzowski/Hilgard Apparatus Method)

Principle

With the capillary rise of water, the soil immediately above a water table gets saturated. The height upto which the soil is saturated depends on the texture, structure and packing of the soil mass. The experimental technique discussed herein permits approximation of maximum water holding capacity while determining the amount of water held in the soil at the point of saturation.

Principle

When water is ponded inside the concentric metallic rings driven into the soil, the rate of recession (fall) of the water level, or the rate of water withdrawn from a supply source used to maintain a constant head of water on the soil surface, gives the infiltration rate of the soil.

During infiltration of water into the soil, appreciable lateral movement of water may also occur. To avoid the error due to this lateral movement, two concentric rings are used. Equal heights of water are to be maintained in both the rings. The infiltration is measured in the inner ring only. The rate of water intake per unit area varies markedly with the size of the rings and the depth of the soil to which they are driven into. Infiltration rate studies are preceded by the profile, initial soil moisture and any other problems of the region in which the soil is located.

(International/Robinson’s Pipette Method)

Principle

This method is based on Stoke’s law. According to this law, the rate of fall of a particle in liquid is directly proportional to the square of its radius.

Bulk density is the mass per unit volume of soil including pore space. The normal range of bulk density in most soils is ranging from 1.02 to 1.80 g / cm³. The bulk density of coarse textured soils will be on the higher side while organic soils will have lower bulk density.

Particle density is the mass of soil per unit volume excluding pore space. Pore space is that fraction of soil volume not occupied by solid particles.

Principle

The bulk density and percent pore space are determined from the apparent and true volumes of the soil measures by adding a known quantity of water to a measuring cylinder contain a weighed quantity of soil.

Principle

Soil moisture content is determined by drying a known quantity of soil sample in an electric oven at 105° C to 110° C and finding out the loss in weight.

Soil is analyzed to know the nature of soil, to classify and advocate to farmers about the peculiarities of the soils and how much of fertilizers should be applied for better crop production.

A composite soil, of course, can not be moved to into a laboratory. The value of the laboratory work depends upon care in sampling. Each soil sample needs to be a fair representative of the specific area or horizon worth sampling.

If the sample is to be a representative of an area, it is necessary to take large number of samples spread over the area, pool them and sub-sample it so as to get a sample of desired size. For soil survey work, samples are collected from a profile which is typical of the soil of the surrounding area.

Principle

Equal volumes of all acids / bases containing their gram equivalent weights per litre of the solution react completely with each other.

More often, if the reagent is not a primary standard, a secondary standard solution must be prepared. The chemical is dissolved and made up or diluted to the desired concentration and then it is standardized against a primary standard or against another reliably known secondary standard solution. Such solutions are called as secondary standard solutions.

Principle

Standard solutions are the solutions of accurately known concentrations. There are two types of standard solutions viz., primary and secondary.

A known quantity of the substances is dissolved in a volumetric flask and the volume is made upto the mark. Standard solutions prepared in this way are known as primary standard. This method can be used for preparation of standard solutions of substances which satisfy the following conditions.

5.1 Volumetric Techniques or Titrimetric Analysis

Acid Base Neutralization Reactions-Acidimetry & Alkalimetry

Oxidation –Reduction Reactions-Oxidometry (Permanganometry) & Dichrometry

Practice to calculate the weight / volume of reagents required to prepare the following standard solutions.

Preparation of Standard Solution - (1 M, 0.1 M, 1 N, 0.1 N, 0.5 N, 5%, 10%, 20%, 10 ppm, 20 ppm, 100 ppm)

Instrumental methods of chemical analysis are the quantitative methods of analysis that use, as principal measuring devices, instruments other than those employed in conventional gravimetric and volumetric determinations.

The main basis for this method of analysis is the physical property of any particular element or compound being analyzed. These properties include:

2.1 Beakers

Beakers are the one of most important vessels used in laboratory which is made up of heat resistant glass.

They are available in various capacities ranging from 10 to 3000 mL.

For convenience of pouring solution, spout is provided in the beaker.

They are used to perform titrations & for boiling solutions, etc.,

Beakers are constructed in tall and low forms.

Analytical chemistry is a branch of chemistry dealing with the determination of constituents of a substance or mixture of substances.

1.1 Qualitative Analysis

Qualitative analysis deals with the identification of the constituents of a substance, mixture of substances, or solutions and the way in which component element or a group of elements are constituted.

1.2 Quantitative Analysis

Quantitative analysis is concerned with the determination of quantitative contents of individual element or group of elements or compound present in the substances. This branch of analytical chemistry is of enormous scope and importance in science, industry and agriculture. The quantitative analysis can be divided into several categories based on the different methods employed for their quantitative measurements such as:

12.1. Women in Agriculture

Women are actively involved in various agricultural activities.

11.1. Definition

Jones: Variations or modifications of any aspect of social process, social patterns, social interaction or social organization.

Gillin and Gillin: Variations from the accepted modes of life; whether due to alteration in geographical conditions, in cultural equipment, composition on the population or ideologies and whether brought about by diffusion or inventions within the group.

Davis and Mac Iver: Change in the relationships.