eChapters

Introduction
Good Laboratory Practices (GLP) are a quality system concerned with the organizational process and the conditions under which non-clinical health and environmental safety studies are planned, performed, monitored, recorded, archived, and reported. GLP ensures the quality and integrity of the study process rather than the scientific validity of the study itself. It provides a framework for ensuring that data generated in non-clinical safety studies are reliable and reproducible, thereby facilitating the mutual acceptance of data by regulatory authorities worldwide (OECD, 1998; US EPA, 2016). GLP represents a structured quality system designed to ensure the consistency and reliability of non-clinical safety studies. It establishes a framework encompassing organizational processes and laboratory conditions, with a focus on:
1. Systematic Study Conduct: GLP mandates a methodical approach to the planning, execution, supervision, recording, reporting, and archiving of studies.
2. Data Integrity Assurance: The primary objective is to guarantee the dependability of data generated, thereby fostering confidence in safety assessments.
3. Regulatory Compliance Support: GLP facilitates adherence to regulatory standards, enabling the acceptance of study findings by authorities worldwide.
4. Non-Clinical Safety Focus: It specifically applies to studies that precede human clinical trials, particularly those evaluating the potential hazards of chemical substances (OECD, 1998).
In veterinary and allied fields, GLP extends beyond general scientific rigor to address the unique challenges of animal health, food safety, and environmental protection related to animal populations. It ensures the reliability and traceability of data generated in studies related to veterinary pharmaceuticals, feed additives, diagnostics, and environmental toxicology involving animals. The focus is on maintaining the integrity of studies that directly impact animal welfare, public health, and agricultural sustainability (World Organization for Animal Health, 2018; FDA, 2010). In the context of veterinary medicine or treatment aspects, GLP in veterinary refers to a a well organised quality system that governs the organization and conditions under which non-clinical studies related to animal health and safety are planned, performed, monitored, recorded, reported, and archived. This includes:

For millennia, humans have forged an intimate relationship with the natural world, recognizing and harnessing the therapeutic potential of plants for healing. This unit, “Introduction to Herbal Medicines,” embarks on a comprehensive exploration of plant-based therapies, delving into their historical evolution, fundamental terminology, integration within traditional healing systems, and the practical aspects of their cultivation, processing, and utilization. We aim to provide a solid bedrock of knowledge, enabling a deeper understanding of the science and practice of herbal medicine, and appreciating its enduring significance in the modern world."
1. History and Scope of Herbal Medicines
1.1 Ancient Beginnings: The Dawn of Plant-Based Healing
• Paleolithic Roots: Our most ancient ancestors, from the Paleolithic period, are believed to have harbored a refined comprehension of the pharmacological attributes of vegetation. Excavations have unearthed indications of specific plant utilization for addressing physical trauma and maladies, underscoring an early grasp of their remedial efficacy. This knowledge was likely passed down through oral traditions, forming the foundation of future herbal practices.
• Civilizational Emergence: With the rise of civilizations, medicinal plant use became more structured and recorded. In ancient Sumeria, clay tablets inscribed with cuneiform script, dating back to 3000 BC, record the use of various plants for medicinal purposes, showcasing organized knowledge of herbal remedies. The Egyptian Ebers Papyrus (c. 1550 BC), a remarkable medical text, provides detailed prescriptions for treating a wide array of ailments using plants like aloe vera, garlic, and opium, evidencing a sophisticated understanding of pharmacotherapy. In India, the ancient system of Ayurveda, with its foundational texts, the Charaka Samhita and Sushruta Samhita, meticulously documents the use of numerous medicinal plants, emphasizing a holistic approach to health. Similarly, Traditional Chinese Medicine (TCM), with the Shennong Bencao Jing (c. 1st century AD), provides a comprehensive pharmacopeia, demonstrating the profound integration of herbal remedies into Chinese medical practice.

Horticulture is an intensive branch of agricultural and plant sciences concerned with the production, management, improvement, utilization, and marketing of high-value crops. It encompasses fruits, vegetables, flowers, ornamentals, plantation crops, spices, medicinal plants, and nursery crops. This chapter introduces the meaning, scope, importance, history, and major branches of horticulture. It explains how horticulture differs from general agriculture through its emphasis on quality, intensive management, perishability, and value addition. The chapter also highlights the economic, nutritional, social, and ecological contributions of horticulture and traces its development from traditional cultivation to modern technologies such as protected cultivation, precision farming, hydroponics, and artificial intelligence.

The term ‘Horticulture’ is derived from two Latin words, Hortus meaning Garden and Cultura meaning Cultivation. In ancient days the gardens had protected enclosures with high walls or similar structures surrounding the houses. The enclosed places were used to grow fruits, vegetables, flowers and ornamental plants. Horticulture differs from agriculture and forestry in specializing the cultivation of garden crops. The garden crops include fruits, vegetables, flowers, ornamental trees, spices, plantations, medicinal and aromatic plants. Horticultural crops do not give food grains. The products of horticultural crops such as fruits and vegetables are highly perishable so that they are utilized in the fresh state. In general horticultural crops need more intensive cultivation (i.e. need more work) than the agricultural crops.

Special Features of Horticulture

As horticultural crops generally need intensive cultivation, they require a large input, capital, labour and technology per unit area of land.

Many cultural operations are specific to horticultural crops. The cultural operations such as propagation, fertilization, training, pruning, harvesting and marketing are skilled operations and these are specific to horticultural crops.

Mostly the horticultural products are utilized in the fresh state and are highly perishable.

Most of the horticultural crops are the rich sources of vitamins and minerals.

Gratification or aesthetic sense is an exclusive phenomenon for horticultural science.

This chapter introduces ergonomics as the science and practice of designing tools, systems and environments around human capabilities and limitations. It explains the meaning, significance and principal objectives of ergonomics, including safety, comfort, efficiency and human well-being. The historical development of the discipline is traced from early toolmaking and industrialization to contemporary human-centred design. Anthropometry is introduced as an essential basis for matching furniture, equipment and workplaces to different users. Examples from domestic life, education and professional settings demonstrate the practical relevance of ergonomic thinking. The chapter establishes human-centred design as the foundation for developing functional, safe and accessible solutions.

Background
Cells are the basic unit of life. The morphology of cells describes their structural framework. It refers to the morphological features of the cell(s). It comprehends the various shapes and sizes in which cells function. Thus, the shape and size of the cell and the number of cells indicate their functional status. Hematological diagnostics requires the proper assessment and valid classification of cells for final diagnosis and prognosis. Clinicians, patients, and diagnosticians are all concerned with achieving a definitive specific diagnosis employing a single investigative test. The utilization of different cytological examinations from different organs gives adequate indicative data that leads to treatment outcomes.
What is ImageJ?
ImageJ is a public-domain image processing and analysis software that is Java-based and developed by the National Institutes of Health. The software is used to process and analyze scientific images.
The key features of ImageJ are 1) the versatility of working with various image formats, 2) extensibility with several plugins, 3) multidimensional data handling capability, and 4) vibrant community, making ImageJ a unique software for image processing and analysis. It can run as an online applet or be downloaded and used as an app.

Introduction

Impact studies isolate the effect of an intervention by assuring that there is clean comparison between a treatment group that received the intervention and a comparison group that is just the same except that it did not get the intervention.

Impact studies test whether one thing causes another. Just knowing scores have gone up following an intervention doesn’t mean improvement is attributable to that intervention.

The data are generated from an impact study used to identify best practices, to replicate successful programs or policies, and to avoid mistakes that might have negative implications for future projects.

Introduction
Impact Assessment (IA) is a systematic process used to evaluate the potential or actual effects of a project, policy, or program on society, the economy, and the environment. It helps decision-makers understand the implications of their actions and ensures that interventions achieve their intended outcomes while minimizing negative consequences.
Meaning of Impact Assessment
Impact Assessment is a structured method of determining the significance of an action or intervention in various sectors. It involves analyzing both the intended and unintended effects of policies, projects, or programs. IA provides insights into the sustainability, feasibility, and effectiveness of an initiative before or after its implementation. By assessing social, economic, and environmental impacts, IA helps stakeholders make informed decisions.
Concept of Impact Assessment
The concept of IA is rooted in sustainable development and evidencebased policy making. It is used in multiple fields, including environmental management, social development, economic planning, and corporate governance. The key principles of IA include:"
i. Comprehensiveness: Evaluating a wide range of impacts, including social, economic, and environmental factors.
ii. Transparency: Ensuring that the assessment process is open and inclusive.

Introduction
As global populations increase, so does the demand for high-quality seafood. Aquaculture has emerged as a vital solution, contributing significantly to global fish production and food security. However, traditional practices in fisheries nutrition, primarily reliant on fishmeal and fish oil, face substantial challenges. Overfishing, rising feed costs, and environmental concerns necessitate a shift towards more sustainable and innovative approaches in aquaculture nutrition. This chapter delves into the innovative methodologies and technologies shaping the future of fisheries nutrition, emphasizing sustainability, health, efficiency, and the optimization of nutritional strategies.

Integrated fish farming is based on the concept that ‘there is no waste’, and waste is only a misplaced resource which can become a valuable material for another product. In integrated farming, the basic principles involve the utilisation of the synergetic effects of inter-related farm activities and the conservation, including the full utilisation of farm wastes. It is assumed that all the constituents of the system would benefit from such a combination. However, in most cases, the main beneficiary is the fishes which utilises the animal and agricultural wastes directly or indirectly as food. As integrated farming involves the recycling of wastes, it has been considered an economic and efficient means of environmental management. Integrated fish farming, also known as rice-fish culture, is an age-old practice that combines aquaculture and rice farming. This method has gained renewed interest due to its potential to increase agricultural productivity and sustainability.

Intellectual Property Rights (IPR) refer to the legal rights granted to creators, inventors, and innovators for their intellectual creations. These rights enable individuals or organizations to control and benefit from the use of their creations for a certain period. In the era of rapid advancements in biotechnology and molecular biology, IPR has emerged as a cornerstone in protecting and commercializing innovations in plant genetics, genetic engineering, bioproducts, and molecular tools.
The concept of IPR is rooted in the recognition that ideas, like physical property, hold economic value and deserve legal protection. IPR plays a pivotal role in encouraging innovation, fostering technology transfer, promoting research investment, and ensuring economic returns to developers. In biotechnology, IPR has direct implications on transgenic technologies, bioinformatics, novel genes, enzymes, diagnostic kits, and biopharmaceuticals.
1.1 Evolution and Historical Background of IPR
1.1.1 Introduction
The concept of Intellectual Property Rights (IPR) has evolved over centuries as a legal framework to protect creations of the human intellect. While the terminology “intellectual property” is relatively modern, the underlying idea—that inventors, artists, and creators deserve recognition and protection for their innovations—has ancient roots. In the context of modern biotechnology, IPR serves not only as a legal safeguard but also as a vital tool to stimulate innovation, secure investments, and promote equitable commercialization.
Understanding the historical trajectory of IPR helps contextualize contemporary debates on the ownership of genetic resources, traditional knowledge, and the ethical implications of patenting life forms.

Intellectual property (IP) refers to the creations of the human mind that can be protected and have commercial value. These creations, or “intellect,” can take various forms, such as inventions, artistic works, and designs. Converting intellectual property into a useful product or process requires resources like time, effort, skill, and financial investment. Intellectual property rights (IPR) are legal rights granted to innovators and creators, giving them exclusive control over the use and monetization of their inventions and creations. IPRs are territorial and allow creators to sell, license, transfer, or otherwise dispose of their rights, similar to physical property.

IPRs are considered crucial for promoting economic growth and development, as they encourage innovation, investment, and entrepreneurship. Without IPRs, inventions and creative works could be easily copied or stolen, resulting in reduced incentives for innovation and decreased economic value. Lack of IPR can also lead to an increase in infringements and economic losses. In recent years, India has been increasing its awareness of IPR and has seen a rise in registrations of indigenous technology, patents, trade secrets, and copyrights among others.

The Internet of things connects the devices over Internet. The devices can communicate with each other and can be monitored and controlled remotely. The IoT is a system with interrelated computing devices, digital and mechanical machines, objects, animals or people each with an unique identifiers (UIDs ). It has the ability to transfer data over a network without human intervention. The Internet of Things is simply a network with Internet connecting the objects to collect and exchange the data. This chapter covers the basics of Internet of Things and its components.

2.1 Characteristics of IoT

Internet of Things can be used to design products for businesses. The added value to the businesses over IoT is created by the information which is collected by connected devices. The process has five phases of lifecycle, first phase is ‘create phase’, where sensors collects the data from environment. This data can generate the information. Second is ‘communicate phase’ where data generated in first phase is communicated to the required destination. Third is ‘aggregate phase’ where collected data over network is aggregated by device itself. Fourth is ‘analyse phase’ where aggregated data is used to generate patterns and use it to control and optimize the process. Fifth is ‘act phase’ where actions are taken on the basis of information extracted from the aggregated data.

The characteristics of IoT may vary from one domain to other. Few characteristics are listed as follows-

To understand IoT “Internet of Things” it is important to understand internet first. Internet is connecting a device to other device anywhere in the world. When two devices are connected with the Internet, they can send and receive all kinds of information such as text, graphics, voice, and video. The high-speed, fiber-optic cables are used, through which the bulk of the Internet data travels. The Internet has revolutionized the communication world. In today’s scenario the internet is an important part for people around the world. The Internet is a global network of billions of electronic devices. The World Wide Web (www) is most widely used part of the Internet.

The “Internet of Things is a potentially integrated part of the ‘Future Internet’. IoT can be defined as a dynamic global network with self - configuring capabilities based on communication protocols where physical and virtual ‘Things’ interact with each other.

The Internet of Things (IoT) is inter-connecting the networks of smart devices. Smart devices may be embedded with vehicles, buildings, or other electronics object with software, sensors, actuators, and network connectivity. Network connectivity enables smart devices to collect and exchange data. The Internet of Things (IoT), also referred to as the Internet of Everything (IoE) which comprises of the web-enabled devices. IoT has its applications in smart city, smart surveillance, automated transportation, smarter energy management systems, water distribution, urban security and environmental monitoring. Agriculture is another new area of IoT application.

Due to advancement in technology the processing power and storage capacity has been increased, in the recent years. At the same time technology is making the devices pervasive, wearable and mobile. Smart devices are fitted with sensors, actuators which enable devices to sense, compute and act intelligently. They are able to exchange information with environment.

This is a branch of veterinary science that deals with the biology of animal when they are in good health condition, disease status and also deals with the selection of the laboratory animal, important care to be taken and also the usage of these laboratory animals in experiment and also for other research activities.

IMPORTANCE / SCOPE FOR STUDY

Veterinarian with special training/ advanced training in laboratory animal medicine and management are required by medical school, pharmaceutical industries, private and government organization, hospitals, research institutes.

This field of veterinary science has become highly dynamic and it provides good opportunities to satisfy academic professional career.

Usage of these laboratory animals goes back to 2^nd Century. However, the systematic and systemic usage came into existence a century back when research workers started working on polio vaccine. Since then, a debate is going on between scientists of different organizations regarding the usage of laboratory animals.

Large Language Models (LLMs) have emerged as one of the most significant developments in artificial intelligence, transforming the way machines understand, process and generate human language. Their evolution reflects a progression from rule-based systems and statistical language models to neural networks, word embeddings, recurrent architectures and, ultimately, Transformer-based models. This chapter provides a comprehensive overview of this evolution and explains the technological breakthroughs that enabled modern LLMs. It discusses early language modelling approaches, the transition from N-gram models to distributed representations, and the contribution of deep learning and attention mechanisms. The development of influential models such as BERT, GPT, T5, XLNet, PaLM and LLaMA is considered to illustrate increasing capabilities in language understanding and generation. The chapter also examines core LLM capabilities, including question answering, dialogue, summarization, translation and multimodal processing. Finally, it highlights challenges involving hallucination, computational requirements, privacy, bias and responsible deployment, while outlining opportunities for future intelligent and trustworthy AI systems.

The science of Meteorology is defined as the science that deals with physical and chemical processes of the lower atmosphere. The physical and chemical changes in the lower atmosphere produce weather and popularly known as the weather science. The weather science concerns the study of structure, state and behaviour of the lower atmosphere. It is essentially an observational science dealing with different atmospheric variables such as atmospheric pressure, temperature, wind, humidity, density, cloud amount, radiation, precipitation and related variables at a given moment and attempts to predict the behaviour of atmosphere over a period of few hours to few days ahead and disseminate the same in time to the users? community. The statistical approach of weather phenomena deals with the mean state of the physical properties of atmosphere. This branch of science is popularly known as Climatology. The weather science can be categorized into several branches based on method of approach, purpose and function, scale and region. Based on the purpose and function, it can be divided into several branches like Livestock Meteorology, Agro Meteorology, Forest Meteorology, Hydro Meteorology and Bio-Meteorology (Fig 1.1).

Animal husbandry is the most important economic activity in rural areas as livestock plays a vital role in the economy. Over 90 per cent of the milk production comes from small farmers, who are located in rural areas. Growing human population, increasing urbanisation, rising domestic incomes and changing lifestyles in the country have led to increasing demand for dairy products (Anita and John, 2001).

Introduction Market led extension effectively delivers sufficient and high-quality information to farmers so they can make decisions about production and marketing that willmaximize their return on investment without endangering the needs of future generations. Farmers must improve from being producers or sellers in the home market areas to producers cum sellers in a large market to best reap the benefits of their products. This necessitates the transfer of new extension strategies involving a range from local market to export markets, subsistence to commercial agriculture, productivity to profitability, commodity-oriented to farming systemsoriented, mono-cropping to varieties of crops, and so forth. The extension agents’ attention must be diverted from production in some way. Reducing production costs, increasing product value, and enhancing marketability will assist the farming community in receiving high returns for the products.