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India, the second most populous country in the world after China, is trying hard to provide basic needs like food, shelter, clothes, electricity and education facility to its 1,316 million people and to upgrade itself from the stage of ‘Developing economy’ to ‘Developed economy’. Table 1.1 depicts the trend of population increase from 1154 million to 1316 million during 2009 to 2018 with diminishing growth rate from 1.42% to 1.12%. Growing demand of ever increasing population has been met so far by over exploiting natural resources like land, water, fossil fuels, forests and mineral resources which resulted rapid unplanned urbanization, quick depletion of fossil fuels, more and more waste generation, alarming increase in environmental pollution, deforestation with loss of plant and animal species, desertification of cultivable land due to soil erosion, soil contamination & soil salinity and increase in health diseases due to malnutrition, starvation and environmental pollution.

An Overview of Fish and Shellfish Health Management in Aquaculture in India

Aquaculture in India in particular freshwater fish culture comprised mainly of Indian major carp cultivation has a long history. Since 1980s commercial carp culture expanded, particularly in Andhra Pradesh and in few years became an Asia model. Then on, it is slowly and steadily expanding to different parts of the country particularly Punjab, Haryana, West Bengal, Orissa and Tamil Nadu and Karnataka. Of late, culture of Pungasius (Basa) has come up on large scale in AP. In addition, there is also growing small scale commercial cultivation of catfish, murrels, tilapia and freshwater prawn(Macrobrachium rosenbergii). Aquarium fish breeding is also expanding to establish itself as an industry in India.

Brackish water culture has been practiced traditionally in India in Pokkali fields of Kerala, Bheries of West Bengal and Ghaznis of Karnataka since hundreds of years. From 1990 onwards, commercial shrimp culture came into existence along the coasts of India. High export value, support from government and short culture period encouraged private entrepreneurs to pursue shrimp farming in a big way. There is vast potential for fish culture in brackish water and at the present efforts are on to popularize culture of Asian seabass, mullets, Eutroplus and milk fish. Mariculture is still at infancy but increasing at a slow and steady rate.

Aquaculture health management has to be viewed from the incidences and outbreak of diseases due to growth and expansion of the industry. Aquaculture has expanded tremendously in the last three decades, growing at 6-7 % annually. Contribution of fish production from aquaculture in the country is around 60 % compared to 10 % it was 40 years ago. In few years to come, aquaculture contribution may go up to 80 % of total fish production. Today 2.43 m ha of freshwater and 1.15 m ha of brackish water are under culture. In addition, 8.62 m. ha of irrigated inland area mainly in Haryana, Maharastra and Karnataka which have become saline and unsuitable for agriculture are slowly being reclaimed for aquaculture. Farmers are growing acclamatized saltwater fish and shrimp spp in inland saline waters and a shift like this may favour pathogens with different virulence to cause disease. Overall, it can be seen that in addition to increased conventional areas in fresh and brackish water, aquaculture is being practiced in unconventional areas like saline soils. Performance of fish and shellfish in these non conventional water bodies in the long run needs to be studied from their health point of view.

India is famous as the ‘Land of Spices’ world over. Spices are nature’s gift to humankind for flavoring our food. They are also used as preservative and in pharmaceuticals. India is the largest producer, consumer and exporter of seed spices and their value added products. About 63 different spice crops are cultivated in the country. Among them, seed spices are annual herbs, whose dried seeds or fruits are used as spices. These crops share 21.87% in terms of quantity and 7.42 % in terms of value to total spices export in the country. The country grows a wide variety of seed spices like coriander, cumin, fenugreek and fennel as major crop, while, dill, nigella, ajwain, anise, celery, caraway, parsley, black cumin and few other are categories as the minor seed spices.

Association of human beings with plants is much older than the dawn of civilization. Use of plants as source of food, cloth and shelter started first for fulfilling basic needs. Aesthetic use by way of growing flowers and ornamental plants came much later. Gradually, it was felt that ornamental plants need to be grown in a systematic manner and that yielded the concept of designing gardens. With the advancement of time, gardening styles metamorphosed in various ways influenced by climatic conditions, geographic locations, rulers and most predominantly by people. At present, plants and gardens are part and parcel of our daily life. The scope of developing gardens is simply enormous, be it home, office, institute, temple, factory, ‘samadhi sthal’ etc. Growing plants and developing gardens are now considered a necessity wherever scope exists.

Seed is the primary and most critical input in any crop production programme. A quality seed alone contributes around 15-20% towards total production. The efficacy of all other inputs to increase production will be in vain if the quality of the seed is poor. Hence, use of quality seed is crucial as it appreciably increase the productivity of the crop without depending much on other inputs. This adds to the old saying in Manu Smriti good seed in good field yield abundantly .A quality seed can be defined as genetically and physically pure seeds with high germination percentage, free from seed borne pathogens and safe moisture content. This guarantees good germination, vigorous growth, and good establishment of crop in the field.

History of Seed Industry in India

Farmers dependent mostly on traditional indigenous varieties earlier and its seed requirements were met through their own farm saved seeds or through local sources. But the quality of these seed stocks was not assured, and this resulted in drastic reduction in production as well as productivity of the crops. As a result of various crop improvement programmes, improved varieties possessing high yield potential along with additional characters of biotic and abiotic resistance came into the scenario. But the availability of the seeds of these improved varieties among the farmers remained as a problem and it limited the cultivation of these varieties. This led to the development of a well-planned systemized seed production system. Government of India had framed and brought out different legislations for regulating systemized seed production. These laws are framed to make available quality seeds and planting materials to a common farmer and equip him to approach authority for justice. Seed legislations were initiated in the country by establishment of National Seed Corporation (NSC) during 1963 under Ministry of Agriculture and the seed industry was restructured through the nationals seed projects phase I (1977-78), Phase II (1978-79) and Phase III (1990-1991). These initiatives strengthened the seed industry through formulation of seed regulations, establishment of state seed corporations and seed certification agencies thereby assured the supply of quality seeds to farmers

The Indian agriculture is characterized by agro-ecological diversities in soil, rainfall, temperature and cropping system. Besides favorable solar energy, the country receives about three trillion cubic meter of water from rainfall. Of the total geographical area of 329 million hectares the net sown area has remained at about 140-142 million hectares during the last 40 years. The gross cropped area however has increased to about 200 million hectare in 2011-2012 (Anonymous, 2013). The total operational holding is estimated at 105 million with average size of the holdings of 1.6 ha. The Indian agriculture is not only characterized by small fragmented land, but also hill farming and shifting cultivation are also practiced. Out of an estimated 142 m ha net cultivated area around 40% is irrigated and rest is rain fed. About 65% of the population depend upon agriculture for their livelihood.

Plants are used as medicine since time immemorial. One of the area of Ethnobotany, Ethnopharmacology is considered as the scientific evaluation of traditional medicinal plants (Cotton, 1996). Cox (1994) has suggested that the ethno-directed sampling is most likely to succeed in identifying drugs used in the treatment of gastrointestinal, inflammatory and dermatological complaints. The Plant kingdom is a virtual goldmine of potential drug targets and other active molecules awaiting to be discovered. It has been estimated that only 10 - 15 per cent of the 7,50,000 existing species of higher plants have been surveyed for biologically active compounds. Natural products produced by plants, fungi, bacteria, insects and animals have been isolated as biologically active pharmacophores. Approximately one-third of the top-selling drugs in the world are natural products or their derivatives often with ethnopharmacological background. Moreover, natural products are widely recognized in the pharmaceutical industry for their broad structural diversity as well as their wide range of pharmacological activities.

New medicines have been discovered with traditional, empirical and molecular approaches (Harvey, 1999). The traditional approach makes use of material that has been found by trial and error over many years in different cultures and systems of medicine (Cotton, 1996). Examples include drugs such as morphine , quinine and ephedrine that have been in wide spread use for a long time, and more recently adopted compound such as the antimalarial artemisinin. The empirical approach builds on an understanding of a relevant physiological process and often develops a therapeutic agent from a naturally occurring lead molecule (Verpoorte, 2000). Examples include tubocurarine and other muscle relaxants, propranolol and other adrenoreceptor antagonists, cimetidine and other histamine H₂ receptor antagonists. With the development of molecular biological techniques and the advances in genomics, the majority of drug discovery is currently based on the molecular approach.

Human beings have been concerned about the concept of food safety since time immorial. Around the globe there has been major improvement in food safety management in the last twenty years. Looking into the progress made on the ways to make food safe and how to make them free from pathogens, this book, will explain food hygiene, the journey of research been taken in food safety till date and the challenges that we are going to face in future to ensure food safety and its wholesomeness. It also includes the role and responsibilities of the various sectors of society, namely governments, food industry, consumers and academia.

In the beginning of the twentieth century many modern food safety policies have been established. In the packing of meat and in food processing industries a lot of work has been done (Sinclair 1906). Another second generation policy includes the outbreak in the United States in early 1990s’ of pathogen E. coli O157:H7 and the BSE scandal of the late 1990s in the UK Lopman et al., (2003) have discussed on viral outbreak in europe. Currently, the crisis has come due to the economic and technological transformations which have taken place in food and the food supply system. As these changes have many health risks the public is very concerned. Therefore, the control forms of safety regulation and food safety laws have come into the picture. These policies include continuous line inspection, visual product inspection and detailed specification of approved hygiene practices. There is a need of a public health-focused policy which works towards the preventive integrated management of foodborne hazards from farm to table. This includes the use of modern science based risk management tools which will increase the efficiency accurately towards public health. A lot needs to be worked out for better implications. Current efforts to modernize food safety policy around the world are being worked out by the use of risk analysis and cost-benefit analysis in public administration and also the quality management in industry. The role of public economics plays an important role in food safety policies. For the social benefits the policy makers need to take decision on the costs and strengthening of the risk assessment. Today there are globalized markets and this can influence the health globally. Emerging economies like China are industrializing and urbanizing rapidly like that of Europe and North America.

Soil is a natural resource that exists on the planet much before the existence of mankind on it. The human relationship with soil throughout the history of mankind has marked its ability of crop production and influenced the success of civilisation. Such relationships also affirm soil as the foundation of agriculture and food security, supplying all the essential ingredients to plants, as fundamentals to life on planet, and support various biogeochemical cycles on earth’ s terrestrial ecosystem. Prosperity of human society depends on the utilisation of planet’ s resources in creative and productive ways. Of these resources, soil has gained much importance as it provides human to sustain themselves with their capacity to food production, to make significant contribution towards biodiversity and other ecosystem services that are vital for the functioning and resilience of earth’ s environment. Soil occupies a pivotal position in the environment to maintain its quality.

Some cyanobacteria (Blue-green algae) like Nostoc are known to work as one of the two coponenents (photosynthetic partner or phycoboint) of lichens. The second partner of lichen is a lichen fungus or mycobiont. The lichens have not been described as a therapeutic agent in Charak Samhita, the monumental work on materia medica prepared by great Acharya Charak and his group in India more than three thousand years ago. However, these have been used in folklore medicine in many countries of the world and have been regarded to bear antibacterial properties (Katiyar and Katiyar, 2009). They are found in tundra even, the treeless plain of the arctic regions of Russia, Siberia, marshy in summer and frozen hard in winter. It is common to see gunny bags full of lichens for sale in the Masala market near Kesa House, Express Canal Road, at Kanpur. They also comprise a part of meat masala (spices for meat). There are references that Raja (King) Todarmal studied algae in relation to purification of water.

However, the search for antibiotics began in the late 1800s, with the growing acceptance of the germ theory of diseases; a theory which linked bacteria and other microbes to the causation of a variety of ailments. As a result, scientists began to devote time searching for drugs that would kill these disease-causing bacteria.

India is basically an agrarian economy where 57.8% of rural households are engaged in agriculture (Situation Assessment Survey of Agricultural Household, 2021). 48.9 per cent of the Indian work force is employed in the agricultural sector, (ILO, 2016). The estimated Indian population for the year 2050 is 1.7 billion which will raise our food demand to ∼400 million tonnes (Rao et al., 2018). In this context, Indian agricultural production system will have to take up the challenge to feed l7.5% of the global population with only 2.4% of land and 4% of the available water resources at its disposal.

History of Chemical Control, Insecticide Toxicology and Properties

History of pest control probably started when the first person swatted a mosquito or pulled off a tick, the battle for the control of our planet didn’t start until organized agriculture, when pests attacked the plants, and we grew to eat and threatened our very survival.

A pesticide is any substance or mixture of substances used to stop, kill, avoid, or lessen the effects of a pest (insects, mites, nematodes, weeds, rats, etc.). This includes insecticide, herbicide, fungicide, and many other substances used to control pests. Pesticide was defined in different ways at different times and in different places. But pesticide is still generally the same: it is a (mixed) substance that is poisonous and effective on target organisms but safe for non-target organisms and environments.

Chemical control, commonly known as pest control, is the use of chemicals to reduce insect populations. Herbicides, fungicides, insecticides, rodenticides, acaricides, nematodes, and fungicides are all examples of pesticides. Insecticides are a class of chemicals used to combat insect pests. A substance or combination of compounds used to kill, repel, or otherwise prevent insects is called an insecticide. When it comes to controlling pests, insecticides are most powerful tools available for management. They have a wide range of positive effects, including high efficiency, speed of healing, general applicability, adaptability to different settings, adaptability to shifting agronomic and ecological conditions, and low cost. When insect pest numbers approach or surpass the economic threshold, insecticides are the sole viable instrument for emergency action in pest management. The usage of pesticides, a widely used method, can be central to such a system. The use of chemical pesticides will remain pivotal in these initiatives. In many cases, chemical control of pests is the best option available. Some people’s view of pesticide use for pest management as an ecological sin is incorrect. Chemical pesticides are a reliable and useful tool for the biologist, so long as they are used in accordance with good ecological principles.

“Hydrology is the science that treats the waters of the earth, their occurrence, circulation and distribution, their chemical and physical properties, and their reaction with their environment, including their relation with living things. The domain of hydrology embraces the full life history of water on the earth.” (Federal Council for Science and Technology, “Scientific Hydrology”, Washington, 1962)

This definition puts in a nutshell what is most often inadequately done before undertaking exploitation of water resources. In short, it is the scientific inputs that are likely to be missed while planning water resources management projects. It is the “full life history of water” part of the above definition that is of specific relevance to the theme of this book, Isotope Hydrology.

Conventional hydrology is strong on measurement and quantification of different parts of the hydrological cycle (Fig.1.1), be it precipitation, run-off, evaporation, transpiration, infiltration, groundwater dynamics etc. Through chemistry and isotopes, we try to understand the processes themselves in order to delineate the history of that part of the hydrological cycle that is being studied. For example, if we are measuring river flow, we would like to know the contribution of different components; base flow, rainfall run-off and snow melt, if any.

Let’s see what we know and what do not know in the conventional hydrological practice. We know surface waters evaporate, form into clouds and precipitation occurs under favourable weather conditions. What we often do not know is the source of moisture, oceanic or inland.

India is well known for its rich flora and fauna and is referred as one of the richest biodiversity nations of the world. The mammalian population of India is a combination of Palaearctic, Oriental and Afro-tropical fauna (Sahajpal et al. 2009). It is the home of 400 different mammalian species, 129 of these species are protected under Wildlife (Protection) Act 1972 (India) and an arbitrator to Convention on International Trade in Endangered Species (CITES) (Nameer, 2015). Illegal trade of the wildlife for preparation of various products is the common issue in the conservation and is also responsible for regional extinction of the species.

Twenty first century is the century of entrepreneurship and every individual can be an agent for innovation and change. Entrepreneurship is regarded as one of the most crucial factors in the economic development of every region of the country. It widens the horizons of economic development even in the socially and industrially backward regions. Dynamic entrepreneurs are considered to be the agent of change in a society. Entrepreneurs play a very important role in generating new employment and setting up new business. The problem of poverty, inequality and regional imbalances can be tackled with the development of entrepreneurship. However, in all economic development activities more and more focus is being centered on entrepreneurship of the people. Entrepreneur has been now recognized as a concept, not only for starting industries but also in the development of dairy enterprise. In India, it is believed that tremendous latent entrepreneurial talent exists which, if properly harnessed, could help in solving many of the serious problems facing the country.

Agriculture depends mainly on natural resources like land situation, soil, water and a favourable climate. Continuous agricultural practices adopted without conservation of such precious resources may lead to unsustainability of growth in agriculture. Conservation of these resources and their sustainable use with appropriate measures would be required for a sustainable agriculture.

Definitions

Pathology

Pathology is the study of the anatomical, chemical and physiological alterations from normal as a result of disease in animals. It is a key subject because it forms a vital bridge between preclinical sciences (Anatomy, Physiology, Biochemistry) and clinical branches of Medicine and Surgery (Fig.1.1). Pathology is derived from the Greek word pathos = disease, logos = study. It has many branches, which are defined as under:

General Pathology

General Pathology concerns with basic alterations of tissues as a result of disease. e.g. fatty changes, thrombosis, amyloidosis, embolism, necrosis (Fig. 1.2).

Systemic Pathology

Systemic Pathology deals with alterations in tissues/organs of a particular system. e.g. respiratory system, genital system etc. (Fig. 1.3).

Introduction

Progress in plant biotechnological research has opened many avenues for basic and applied research in the field of crop plants. Plant tissue culture is an important component of biotechnology that involves in the improvement of crops. Besides this, plant tissue culture provides a good system for many basic studies in plant breeding, plant physiology, genetics and cell biology. Further, manipulation of cells through the modern technique of genetic engineering may lead to an introduction of new gene(s) to a host plant and the techniques of genetic engineering rely on plant tissue culture techniques. The regeneration of plants through plant tissue culture and their subsequent acclimatization and delivery to the field possesses many problems to make tissue culture technology a viable alternative proposition. The successful development of encapsulation technique for preparation of plant propagules in a nutrient gel has initiated a new line of research on artificial seeds. Application of this technology has been well recognized in several agronomically important crops and forest trees. Artificial seeds make a promising technique for propagation of transgenic plants, non-seeds producing plants, polyploids with elite traits and plant lines with problems in seed propagation.

Part A

1. Pesticides

A pesticideis defined as anysubstance intended for preventing, destroying, attracting, repelling or controlling pests including unwanted species of plant, or animals duringthe production, storage,transportation, distributionand processing of the food, agricultural commodities or animal's feed or which may be administered to the animals for the control of ectoparasites. Pesticides include insecticides, herbicides, fungicides, acaricides, nematicides, etc.

According to food and agricultural organization (FAO) 1986, the term pesticidehas been defined as“any substance or mixture of substances intended for preventing or controlling any pest and includes anysubstance or mixture of substances intended for use as plant growth regulator (PGR), defoliant or desiccant. The term excludes fertilizers and antibiotics or other chemicals administered to animals to stimulate their growth or to modify their reproductive behaviour”. Pesticides are the largest group of poisonous substances that are widely broadcast today.

Crop production broadly depends on the fertility of the soil where a crop is raised. There are several factors like the kind and quality of seed, climate, water supply, and plant protection measures etc. that affect the crop production. Among these factors, the fertility status of the soil largely determines the ultimate yield even if all these factors are optimum for crop production. A clear distinction should be made between soil fertility and soil productivity. Soil fertility deals with the inherent capacity of soil to provide nutrients in adequate amounts and in proper balance, for the growth of specified plants when other growth factors such as light, water and temperature, and the physical condition of the soil are favourable. Soil productivity is concerned with the capability of soil to produce a specified plant or plant parts or a sequence of plants under well defined and specified systems of management inputs and environmental conditions, viz. climatic conditions. It does not mean that a fertile soil is always productive. A fertile soil, under a bad management system, may be unproductive for a crop. In this book, plant nutrient elements and their available forms, functions and deficiency symptoms are briefly described for the better understanding of soil testing and fertilizer recommendation.