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Mycotoxins are naturally occurring toxins produced by certain molds and can be found in food. The molds grow on a variety of different crops and foodstuffs including cereals, nuts, spices, dried fruits, apples and coffee beans, often under warm and humid conditions. Mycotoxins can cause a variety of adverse health effects and pose a serious health threat to both humans and livestock. The adverse health effects of mycotoxins range from acute poisoning to long-term effects such as immune deficiency and cancer. A scientific expert committee jointly convened by WHO and the Food and Agriculture Organization of the United Nations (FAO), called JECFA, is the international body responsible for evaluating the health risk from natural toxins including mycotoxins. International standards and codes of practice to limit exposure to mycotoxins from certain foods are established by the Codex Alimentarius Commission based on JECFA assessments.

Mycotoxins are toxic compounds that are naturally produced by certain types of molds (fungi). Molds that can produce mycotoxins grow on numerous foodstuffs such as cereals, dried fruits, nuts and spices. Mold growth can occur either before harvest or after harvest, during storage, on/in the food itself often under warm, damp and humid conditions. Most mycotoxins are chemically stable and survive food processing. Several hundred different mycotoxins have been identified, but the most commonly observed mycotoxins that present a concern to human health and livestock include aflatoxins, ochratoxin A, patulin, fumonisins, zearalenone and nivalenol/deoxynivalenol. Mycotoxins appear in the food chain as a result of mold infection of crops both before and after harvest. Exposure to mycotoxins can happen either directly by eating infected food or indirectly from animals that are fed contaminated feed, in particular from milk.

In managing diseases of horticultural crops, pathogen quiescence has important implications in timing prophylaxis, the reduction of stresses that trigger the transition of quiescence to aggression, and managing the harvested crop to prolong quiescence to the point at which yield is no longer affected. The inception of pathogen quiescence and its maintenance on or within the host implies a dynamic equilibrium between the host, the pathogen, and their environment. Physiological and physical changes in the host, its environment, or both trigger changes in that equilibrium to permit the pathogen to resume aggression. Microorganisms lying on the host surface constitute a potential latent infection; as can be the case with Botrytis cinerea conidia that are prevented from germinating by antagonistic bacteria on leaf surfaces (William R. Jarvis, 1994). Quiescence can run the spectrum from the ungerminated spore; through symptomless, internal infections; to visible but nonexpanding lesions, such as ghost spot of tomato (Lycopersicon esculentum) to expanding lesions on banana caused by Colletotrichum musae and on avocado caused by C. gloeosporioides.

A latent, dormant, or quiescent parasitic relationship is a condition in which the pathogen spends long periods during the host’s life in a quiescent stage until, under specific circumstances, it becomes active. Epidemiologists might refer to the period from spore landing until the parasitized tissue produces new spores as a latent period (Dov Prusky, 1996). The term “period of quiescence” is referred to differentiate the quiescent parasitic relationship from the latent period. Quiescence can be enforced during the various processes of fungal attack, which suggests that quiescence may or could occur during any of the processes leading from fungal germination to colonization.

Postharvest handling is the stage of crop production immediately following harvest, including cooling, cleaning, sorting and packing. The instant a crop is removed from the ground, or separated from its parent plant, it begins to deteriorate. Postharvest activities include harvesting, handling, storage, processing, packaging, transportation and marketing. Losses of horticultural produce are a major problem in the postharvest chain. The diseases which develop on harvested parts of the plants like seeds, fruits and also on vegetables are the postharvested diseases. The amount or extent of damage depends mainly on the pathogen(s) involved, on the condition of the products and the condition of storage. Major tips to avoid postharvest losses include Tips to avoid postharvest losses include assessing maturity, to check your water quality, to check water temperature, avoid injury, keep the produce cool and proper storage.

“Storage” means the phase of the postharvest system during which the products are kept in such a way as to guarantee food security other than during periods of agricultural production at the marketing level, to balance the supply and demand of agricultural products, thereby stabilizing market prices. Grain, which includes dry kitchen ingredients such as flour, rice, millet, couscous, cornmeal, and so on, can be stored in rigid sealed containers to prevent moisture contamination or insect or rodent infestation. For kitchen use, glass containers are the most traditional method. Maturity indices are the sign or indication the readiness of the commodity for harvest. It is the basis for determining harvest date. Postharvest losses occur between harvest and the moment of human consumption. They include on-farm losses, such as when grain is threshed, winnowed, and dried, as well as losses along the chain during transportation, storage, and processing.

Postharvest loss of fruits and vegetables is defined as “that weight of wholesome edible product (exclusive of moisture content) that is normally consumed by human and that has been separated from the medium and sites of its immediate growth and production by deliberate human action with the intention of using it for human feeding but which for any reasons fails to be consumed by human.” Not only quantity and quality but even the appearance of fruits and vegetables are affected, and their market value is reduced. Most ‘skin deep’ injuries such as ‘fly speck’ in apples do not affect the edible part. Some infections are not harmful as they occur on inedible parts and can be trimmed off before consumption ego Colletotrichum spots on the outer leaves of cabbage]. Fresh fruits and vegetables are perishable and highly prone to these losses because they are composed of living tissues. These tissues must be kept alive and health throughout the process of marketing. These are composed of thousands of living cells which require care and maintenance (Alao, 2000). The pathological rots are the most serious which is followed by mechanical injury. Pathological rots in combination with mechanical damage cause serious damage to the perishables. Environmental factors such as temperature, relative humidity and oxygen balance most especially during storage are also greatly responsible for damage also environmental conditions such as temperature and humidity are responsible for rendering fruits and vegetables susceptible for pathological attacks (Yahaya and Mardiyya, 2019). Fungi and bacteria are the major pathogens, which cause postharvest diseases in crops leading to about 30 % loss. Normally diseases will be more at improper temperature and relative humidity. Apart from causing disease, pathogens are also involved in ethylene production, which hastens the deterioration of the product.

Fruits, nuts and vegetables play a significant role in human nutrition, especially as sources of vitamins, minerals, dietary fibre, and antioxidants. Increased consumption of a variety of fruits and vegetables on a daily basis is highly recommended because of associated health benefits, which include reduced risk of some forms of cancer, heart disease, stroke, and other chronic diseases. Both quantitative and qualitative losses occur in horticultural commodities between harvest and consumption. Qualitative losses, such as loss in edibility, nutritional quality, caloric value, and consumer acceptability of fresh produce, are much more difficult to assess than are quantitative losses. Quality standards, consumer preferences and purchasing power vary greatly across countries and cultures and these differences influence marketability and the magnitude of postharvest losses. Postharvest losses vary greatly across commodity types, with production areas and the season of production. Losses of fresh fruits and vegetables in developed countries are estimated to range from 2 percent for potatoes to 23 percent for strawberries, with an overall average of 12 percent losses between production and consumption sites. In contrast, the range of produce losses in developing countries varies widely. Losses at the retail, food-service, and consumer levels are estimated at approximately 20 percent in developed countries and about 10 percent in developing countries (Adel A. Kader and Rosa S. Rolle, 2004 ). Overall, about one third of horticultural crops produced are never consumed by humans. Reduction of postharvest losses can increase food availability to the growing world population, decrease the area needed for production, and conserve natural resources. Strategies for loss prevention include use of genotypes that have longer postharvest-life, use of integrated crop management systems and good agricultural practices that result in good keeping quality of the commodity and use of proper postharvest handling practices in order to maintain the quality and safety of fresh produce.

Food waste and loss is an enormous issue worldwide. The FAO states that ‘Hunger is still one of the most urgent development challenges, yet the world is producing more than enough food’, but a vast amount of what is produced gets lost on its way from the field to the consumer. According to the FAO, about 30% of cereals, 20% of dairy products, 35% of fish and seafood, 45% of fruits and vegetables, 20% of meat, 20% of oilseed and pulses and 45% of roots and tubers are lost or wasted. Postharvest food loss is defined as measurable qualitative and quantitative food loss along the supply chain, starting at the time of harvest till its consumption or other end uses. Food losses can either be the result of a direct quantitative loss, for example, during the process of harvest or transport or arise indirectly due to losses in crop quality such as undesired sprouting or water loss. Postharvest food loss consists of many factors, which have not changed much in the last 40 years and so postharvest food loss in general has not changed much. Figure 1 illustrates the main reasons for food loss along the supply chain and is based on ‘The food pipeline’ published by Bourne in 1977. Food loss is highest during storage due to pathogens (insects, bacteria and moulds), environmental conditions (e.g. rain, humidity, heat and frost), sprouting and quality loss (rancidity, water loss and saccharification) or animals (rodents and birds). Recently published data for Switzerland have shown that about 53%-55% of the initial fresh potato production and 41%-46% of the initial processing potato production are lost mostly due to pathogen infection, saccharification, water loss of tubers and early sprouting during storage (Buchholz et al, 2018).

10.1 INTRODUCTION

‘Tissue culture’ is commonly used as a blanket term to describe all types of plant cultures, viz., callus, cell, protoplast, anther, meristem, embryo and organ cultures (George, 1993). It relies on the phenomenon of cell totipotency. The different techniques of culturing plant tissues may offer certain advantages over traditional methods of propagation.

Growing plants in vitro in a controlled environment, with in-depth knowledge of the culture conditions and the nature of the plant material, ensures effective clonal propagation of genetically superior genotypes of economically important plants. Micropropagation has become an important part of the commercial propagation of many plants (George and Sherrington, 1983; and Zimmerman et al., 1986) because of its advantages as a multiplication system (Pierik, 1997 and Razdan, 2003). Several techniques for in vitro plant propagation have been devised, including the induction of axillary and adventitious shoots, the culture of isolated meristems and plant regeneration by organogenesis and/or somatic embryogenesis (Williams and Maheswaran, 1986; and Gautheret, 1985).

Tissue Culture is becoming as an alternative means to vegetative propagation of plants. In vitro growing plants are usually free from bacterial and fungal diseases. Virus eradication and maintenance of plants in virus free stage can also be rapidly achieved in cultures (Sharma, 2016).

9.1 INTRODUCTION

When a new individual arises from a somatic cell without formation of a seed, it is called vegetative propagation. Vegetative propagation occurs naturally (i.e., natural vegetative propagation) in many crops, and profitably exploited in horticulture using either conventional or biotechnological artificial techniques. We shall discuss conventional artificial vegetative propagation in this chapter including cuttings, grafting, budding, layering, suckering, etc. They have been widely used for commercial propagation of fruit trees and ornamental shrubs.

8.1 INTRODUCTION

Natural methods of vegetative propagation include strategies that the plants have developed for self-multiplication or perpetuation. Natural vegetative propagation is mostly a process found in herbaceous and woody perennial plants, and typically involves structural modifications of the stem, although any horizontal, underground part of a plant (whether stem, leaf, or root) can contribute to vegetative reproduction of a plant. In crops like ginger, turmeric, onion, garlic, gladioli, and dahlia etc. they continue to grow from buds that are present on the surface of the stem. In some plants, like sweet potato adventitious roots can give rise to new plants. In Bryophyllum and Kalanchoe, the leaves have small buds on their margins. On detachment from the plant, they are able to grow into independent plants and they may also start growing into free plants if their leaves were made to grow in the soil. In nature, vegetative reproduction occurs through underground stems, sub-aerial stems, bulbils, leaves and even roots.

7.1 INTRODUCTION

Grafting and budding are horticultural techniques used to join parts from two or more plants so that they appear to grow as a single plant. In grafting, the upper part (scion) of one plant grows on the root system (rootstock) of another plant. In the budding process, a bud is taken from one plant and grown on another. The practice of grafting can be traced back 4,000 years to ancient China and Mesopotamia. As early as 2,000 years ago, people recognized the incompatibility problems that may occur when grafting olives and other fruiting trees.

7.2 ROOT STOCK

The part of the graft that provides root system to the grafted plant is known as rootstock. It is, normally, raised by seeds in the seedbed and then, transplanted in the nursery bed for budding and grafting. Rootstocks are also raised in pots and polythene bags.

6.1 INTRODUCTION

The ultimate success of an orchard enterprise largely depends upon the quality and genuineness of planting materials as nursery plants are the foundation of the orchard. Fruit crops, mostly being perennial in nature, call for utmost care in selection of cultivar, quality planting material and adoption of right technology for mass multiplication of planting material. Mistakes committed during the initial establishment of orchards cannot be corrected and will cause serious damage to production and productivity. The variation of scion-wood and rootstock has great bearing on the productivity of an orchard. Thus, it is of immense importance having all plants carefully labelled or otherwise marked. Cuttings taken from the mother plant will grow to have the same genetic qualities as the original plant. This is why the plants resulting from these cuttings are called “clones”. When a stem cutting is taken from a mother plant, the piece of stem will produce new roots if it is properly hydrated. Additionally, some plants can be grown from leaf pieces, or leaf cuttings and these will produce both stems and roots.

Thus, the progeny trees are the basic component for the multiplication of fruit plants and ultimately deciding the quality and productivity of the fruit crops. Hence, it is necessary to stock the true to type and best quality progeny trees.

In asexual methods of plant propagation, plant parts are being used as propagating materials. The plant from which the plant parts are collected is known as mother plant. There is every possibility of transmission of disease or pest which is present in the mother plant to the progeny. The mother plants have to be maintained properly to obtain disease free planting materials so that multiplication of disease/pest can be prevented. Thus, there is a need for the establishment of mother plant block/scion banks.

5.1 APOMIXIS

Apomixis is derived from two Greek words “Apo” (away from) and “mixis” (the act of mixing or mingling). It refers to the occurrence of an asexual reproductive process in the place of normal sexual processes involving reduction division and fertilization. During sexual reproduction, developmental steps occurring inside the ovule produce the female gametophyte (embryo sac) and following a double fertilization event give rise to embryo and endosperm structures. Apomixis can be best described as the reference to the asexual process. In some species, embryo sac is produced not as a result of meiosis and fertilization but by certain asexual processes. The occurrence of such asexual reproductive process in the place of normal seed reproductive processes of reduction division and fertilization is known as Apomixis. Such seedling plants produced in this manner is known as apomicts. ’’Apomixis’’ is used synonymously with ’’agamospermy’’ (Stebbins 1950), referring to the asexual production of seed.

Apomixis (asexual seed formation) is a phenomenon in which a plant bypasses the most fundamental aspects of sexual reproduction—meiosis and fertilization—to form a viable seed. Plants can form seeds without fertilization, and the seed genotype is consistent with the female parent. The development of apomictic technology would be revolutionary for agriculture and for food production as it would reduce costs and breeding times and also avoid many complications typical of sexual reproduction (e.g., incompatibility barriers) and of vegetative propagation (e.g., viral transfer). The application of apomictic reproductive technology has the potential to revolutionize crop breeding. Apomixis is widely distributed among higher plants. More than 300 species belonging to 35 families are apomictic. It is most common in Gramineae, Compositae, Rosaceae and Rutaceae. Among the major cereals maize, wheat and pearl millet have apomictic relatives. Apomixis is reproduction where only one parent passes genes to the offspring (King, 2006). Apomictically produced offspring are genetically identical to the parent (clones).The method is mostly seen in plants, especially flowering plants. Apomixis was defined by Hans Winkler (2008) as the replacement of the normal sexual reproduction by asexual reproduction, without fertilization. Propagation from cuttings or leaves, is not apomixis. Natural replacement of the seed by an asexual method is apomixis.

4.1 INTRODUCTION

The method of propagation in plants may be sexual or asexual. In sexual method of propagation, specialized reproductive cells called gametes are formed by the process of gametogenesis. Fusion of the male and female gametes leads to the development of an embryo and eventually the seed.

4.2 GAMETOGENESIS

The production of male and female gametes is known as gametogenesis. It occurs within microspores and megaspores. It involves only mitosis. It is of two types: (1) microgametogenesis and (2) megagametogenesis.

3.1 INTRODUCTION

A nursery is a place, where seedlings, saplings, trees, shrubs and other plant materials are grown under intensive care and maintained until they are ready for final transplanting in the main field. The importance of the best quality planting material as an initial investment is a well-realized factor for persons engaged in horticulture field. So, nurseries have great demand for the production of plants, bulbs, rhizomes, suckers and grafts. But in general, good quality and assured planting material at reasonable price is not available in most of the cases. Nursery is the basic need of horticulture. Plant propagation techniques and practices is the core of horticulture nurseries. The planting materials for horticultural plantations are raised from seeds and vegetative parts. The difficulty of procuring seeds and their rising cost makes it necessary to find means to increase seedling survival and growth.

2.1 INTRODUCTION

Several propagation structures are helpful in propagation of horticultural crops. These structures are helpful for seed germination, rooting of cuttings, layering, grafting etc. and other hardening operations. The structures which facilitate propagation of plants are called propagation structures. Propagation structures are required for propagating plants by seed, cuttings and grafting.2.2

GREENHOUSE

Greenhouse is a structure covered with glass or polythene for protection against adverse climatic conditions as well as to provide optimum environment for growth of the plants. Greenhouse has been used long back by horticulturists as a means of forcing rapid growth of plants and extending the growing season particularly in colder areas. Plants are grown or cuttings are rooted in a greenhouse when the atmospheric condition does not permit for raising them in outdoor conditions. Modern greenhouses are equipped with regulatory mechanisms for controlling temperature, light intensity, air, humidity etc. Plants in green house are grown in the ground bed or bench type raised beds. The size of the greenhouses and dimensions of beds vary according to the need of propagators. Glass covered greenhouses are expensive but they have long life. However, for short-term benefits, plastic covered greenhouses can also be made. Plastic covered greenhouses are lighter than glass covered ones but there is increase in humidity is such houses, especially in winters, which results in undesirable water drops on the plants. It can however be overcome by making adequate ventilation. Polyvinyl chloride (PVC) has also been used in construction of greenhouses but it tends to darken pre-maturely in sunlight. In India, construction of temporarily low-cost poly houses is in fashion for raising nursery of fruit plant in off-season. Such low-cost greenhouses are constructed either on wood or metal framework and are covered with polyethylene sheet of 0.10 to 0.15mm thickness, which is resistant to ultra-violet rays. These houses are equipped with thermostat, cooler or an air conditioner or humidifier etc for rigid control on temperature and humidity. Greenhouses made from fibre glass sheet are more durable than the polythene houses, but are quite expensive. In greenhouses, two types of beds are used for raising seedlings. In greenhouses, the beds may either be prepared on ground itself or raised beds or bench type beds are used. Greenhouse structures vary from elementary home constructed to elaborate commercial installations.

1.1 WHAT IS PLANT PROPAGATION?

Propagation of plant is defined as multiplication and perpetuation of new individuals from a selected plant having all the characters of the original one. It is the process of increasing the number of plants of a particular species or cultivar. It refers to the multiplication of an individual plant or group of plants, which have specific value to mankind. New plants or new individuals are required for establishing new plantings/new gardens/new orchards.

Ornamental plants that are adapted to an aquatic environment are commonly grown in water gardens and pools. They include those that are entirely submerged or floaters that are free living in the surface water or having roots anchored firmly in the ground beneath and marginal plants that thrive along the edges of water bodies and in shallow water. Spectacular flowering plants like the lotuses and water lilies to plants like the tape grass that are entirely submerged and grass like are grown in natural or structured fresh water habitats throughout the world. Many of these are also inhabitants of aquaria.

Some of the popularly grown water garden plants are listed below:

Acorus calamus

Araceae

Sweet Flag, Vayambu

Plants are rhizomatous herbs adapted to low lying and wet situations. The leaves are closely spaced, distichously arranged and resembling a fan, leathery, dark green, smooth and shiny. The inflorescence is a spadix that emerges from the middle of the terminal leaf. Plants are valued medicinally for their aromatic rhizomes and are also commonly grown as marginals of water gardens.

Grasses, bamboos, sedges and rushes are plants that are being used increasingly in xeri-scaping and in marsh gardens for getting the benefits of their unusual foliage textures and colours. Their hardiness, stature and canopy frame make them more suited for specimen planting in gardens and landscapes.

GRASSES

Grasses are the most successful of monocots belonging to the family Poaceae that are found the world over. Low growing perennial grasses are used to make attractive lawns in gardens and to provide a desirable turf cover for various situations. A number of species belonging to various genera differing in leaf colour, shape and growth pattern are used for this purpose. Mixtures of different varieties of grasses are also used for making turf having desirable blends of textures and hardiness. Tall bushy grasses having a mound-like or fountain–like habit and fine to velvety surfaces are grown for their textural and architectural features in landscaping. Many are grown as specimen plants for their display value at flowering. In selecting grasses for gardens, their potentially invasive nature is to be taken into account and seed dispersal controlled by timely cutting.

Grass species that thrive in the hot and humid tropics are called warm-season grasses and include Bermuda grass, Carpet grass and St. Augustine grass. Cool season grasses that are adapted to temperate regions and thrive at temperatures of 10 to 20^oC include Blue grass, Fescues and Zoysia.

Pteridophytes such as ferns belonging to several families, selaginellas popularly called ‘club mosses’ belonging to Selaginellaceae and the Lycopods which resemble both these, belonging to Lycopodiaceae are shade loving plants adapted to cool and moist or hot and humid ecosystems of tropical to temperate climates. They are mostly epiphytic or lithophytic and are grown as foliage ornamentals in a fernery or in shaded areas in gardens. Many are favoured indoor plants.

Popularly grown ferns, lycopods and selaginellas of gardens are listed below:

Adiantum capillus-veneris

Adiantaceae

Common Maidenhair fern

Plants are shade loving bushy lithophytes and have delicate looking thin black stems carrying light green oblique-ovate fronds. They are pot grown as ornamental foliage plants.

Adiantum decorum

Syn. Adiantum raddianum

Adiantaceae

Maidenhair fern

Terrestrial or epiphytic members of the family Bromeliaceae that are grown in gardens for their unusually coloured and patterned leaves or showy inflorescences and fruits are referred to as ornamental ‘Bromeliads’. Being low growing, they are ideally displayed in containers or as epiphytes. They are sun loving, suckering in habit and have xerophytic adaptations. The leaves are tough and fibrous often with spiny margins and sheathing leaf bases that hold water. A grayish-white waxy or powdery coating often covers the lamina. Certain bromeliads have a water collecting depression at the center. Inflorescences are sunken, erect or reclining, with condensed or long and much branched stalks carrying colourful bracts and usually, less showy flowers. In many, the terminal leaves become brightly coloured at flowering. These and the floral bracts remain showy even after the flowers are shed.

Ornamental bromeliads popularly grown in gardens include the following:

Aechmea blanchetiana

Copper leaf bromeliad

Plants have strap like leaves that develop streaks of coppery red colour on the upper surface in bright sunlight.

Aechmea burle-marxii

Burle-Marx bromeliad