eChapters

Labile fractions of soil carbon (C) refer to the fraction of soil carbon that are easily altered by microbial activities. These fractions serve as sensitive indicators to changes in management induced soil quality and are grouped under active soil C pool. The method described by Weil et al. (2003)estimates changes in biologically active soil C using a dilute KMnO . This is an improvement over the method used by Blair et al (1995), where a very high concentration (0.333 M) of KMnO₄ was used.

Organic carbon present in soil can be very labile, labile or non-labile. This procedure measures the extent of lability of soil organic carbon through relative degree of oxidation by K₂Cr₂O₇.

Lablab purpureus usually knowna s Dolichus bean, Hyacinth bean or Field bean belonging to the family fabaceae, one of the most ancient crops among cultivated plants. It is a bushy, semi-erect, perennial herb, showing no tendency to climb. It is mainly cultivated either as a pure crop or mixed with finger millet, groundnut, castor, corn, bajra or sorghum in Asia and Africa. It is a multipurpose crop grown for pulse, vegetable and forage. The crop is grown for its green pods, while dry seeds are used in various vegetable food preparations.

Season:Seed is very sensitive to weather. A grain crop can be sold for a lower price even if it damaged by rain. But a seed crop, if affected will lead to low seed quality. Hence selecting the right season is necessary. Generally, the seed should mature in cool dry climate. Seasons should be selected with this idea in mind. In Tamil Nadu the best seed crops are grown during March--April and September - October as the seeds mature during the cool months of January - February.

An animal, which has more or less of comparable (similar) physiology and body composition with various biological system as human beings, which are tiny, easy to handle, less expensive and co-operative are called as “Laboratory Animals”. Importance of Laboratory Animal in Bio-Medical Research Laboratory animals are used in basic biological research, disease diagnosis, immunology, virology, applied medicine and demonstration/teaching. Materials collected from diseased animals need confirmation through microbiological and pathological means of diagnosis. Rats, Beagle dogs and primates are engaged in toxicological research. These are used to study the functions of organs, response of a particular organ or the whole system to drug and the method of evolving surgical techniques and demonstration of experimental surgery need experiment animals. Hamster have cheek pouches which may extend to halfway down the length of the body, are used to temporarily store food and, when full, should not be mistaken for a tumor. An entire shelled peanut

Introduction
 
Animals used for experimental research purpose, which show results resembling to human beings are known as lab animals. They are mostly used because of their small size, lesser feed and space requirement. Furthermore, they are not natural host for most of the animal / human pathogens. The laboratory animal research is conducted at universities, medical colleges, pharmaceutical companies, farms, defense establishments and commercial facilities that provide animal-testing services to industry. It includes basic research in the area of genetics, developmental biology, behavioural science etc., as well as applied research in the area of biomedical science, xenotransplantation and drugs including cosmetics testing. Laboratory animals are also used for education, breeding and defense research. The common laboratory animals are mice, rats, hamsters, guinea pigs and rabbits. However, cats, dogs, monkeys, goats, sheep, cattle, pig, horses, fishes etc. can also be utilized as laboratory animals for experimental purpose in biomedical sciences.

The group of animals constantly used in laboratories for general research in all discipline of science is called as laboratory animals. Commonly, it includes rats, mice, rabbits and guinea pigs. However, under certain circumstances, additional animal species like hamsters, non-human primates, amphibians, fowl, sheep and pigs can also be included. This term incorporates traditional laboratory animals, farm animals, wildlife and aquatic animals. Nontraditional animals, inclusive of invertebrate species are also included where they are relevant to the unit’s mission. Generally, these animals come under vertebrate but broadly laboratory animals may be classified in two major groups i.e. laboratory mammals and non-mammalian laboratory animals. The laboratory mammals include mouse, rat, guinea pig, rabbit, cat, dog, ferret, gerbil, bats, hamster, mink and monkey (Rhesus). Beside these mammalians the large domestic animals/livestock are also used as laboratory animals (horse, cow, sheep, goat etc) for fulfilling the demands of various laboratories. Poultry is also used as non-mammalian laboratory animals and Specific Pathogen Free (SPF) chicks are at utmost demand for production and testing of biologicals. The other non-mammalian laboratory animals includes reptiles (tortoise, lizzards and snakes), insects (cockroach, drosophila, house fly, house cricket, locusts, tsetse fly, stablefly etc) pisces (fishes and snails) and amphibians (toad and frog). Laboratory animals have been used extensively for basic research in drug and bacteria screening, toxicology, antibiotic sensitivity testing, animal models of diseases and production/testing of biologicals. Laboratory mammals are also valuable for diagnosis of infectious diseases in man and domestic animals. In this chapter the most commonly used laboratory animals i.e. mice, rats, guinea pigs and rabbits will be discussed.

Several substrates, like proteins (enzymes), are very sensitive to alteration in the concentration of the solution they are placed in. Hence is the importance of a buffer which controls the chemical properties of the medium. Literally a “buffer” has the ability to resist changes in the hydrogen ion concentration. Although, buffers do contain other molecules, that might, in addition to the hydrogen ion concentration, influence the ionic strength, the activity of enzymes, and other parameters of the experiment.

In any biological experiment, appropriate buffer must be selected based on their effect on the experiment. Ideal buffers control pH and ionic strength without interacting adversely with the experiment undertaken. For example, although phosphate is a common buffer, it may not be suited when phosphate is a substrate or product of the reaction being studied. Also, some proteins interact poorly with some buffers. For example, Tris is not ideal as it exhibit high pKa and the large change in pKa when temperature changes, although it is cheap and most proteins are stable in Tris buffers, moreover it seldom reacts with reaction substrates hence, Tris buffer is commonly used in biochemical experiments.

A. Intestinal protozoa Fresh faeces must be used as a sample. The sample must be examined immediately and without refrigeration. Methods of examination 1. Direct smear examination A thin smear of faeces mixed with either water or normal saline (recommended for Trophozoites since they stay intact and motile longer). Select blood-tinged mucus or tiny tissue bits if the faeces are dysenteric. One to two percent of an aqueous eosin solution can be used in place of saline or water, but the oocyst still resist the stain. The viability is examined using the eosin solution. It is possible to employ an iodine solution (which kills protozoa), which allows for the identification and differentiation of the protozoa. It is possible

Molecular biology labo-ratories are becoming very common in every University and research institutes and a good laboratory manners is extremely important as many of the equipments are very expensive and sensitive. Hence familiarity with equipments becomes very crucial before starting any experiment.

Microscope

A microscope can be defined as an optical instrument consisting of a lens or a combination of lenses for getting magnified images of minute objects. A simple microscope is essentially an ordinary magnifying lens held on an adjustable stand. It can give magnification up to 20 times the size of the object.

A compound microscope consists of two separate lens system, the objective and eye piece (ocular) mounted on a holder known as body tube and with adjustment for illuminating the objects. It gives much greater magnification (100 to 1500 times) when compared to a simple microscope.

This chapter introduces the essential components of laboratory work that form the foundation for veterinary parasitology and molecular biology research. A well-equipped laboratory along with proper safety protocols and correctly prepared reagents, is the key to reliable experimental outcomes and a secure working environment.
The following sections describe the standard operating procedures and laboratory biosafety, standard equipment used in research work, and the fundamental principles of solution preparation. These basic laboratory skills are essential prerequisites for the specialised techniques covered in subsequent chapters on veterinary parasitology, molecular biology and bioinformatics. Understanding and applying these foundational concepts allows researchers to maintain experimental consistency, ensure reproducible results, and conduct investigations safely and efficiently. The methods and principles outlined here will be regularly applied throughout the various techniques detailed in this manual, serving as essential knowledge for all laboratory personnel as well short-term visitors.
1.1 Laboratory Standard Operating Procedures and Safety
Laboratory safety is fundamental to protecting personnel, maintaining sample integrity, and ensuring reliable experimental outcomes. This section outlines essential laboratory conduct, safety protocols for personnel and equipment, waste management, and emergency protocols for standard laboratories carrying out diagnostics as well as research.

In the Radiochemical Laboratory

The following are some illustrative recommendation which may be used selected as a guide for setting up working rules :

General

No unnecessary materials should be brought into the laboratory. No unnecessary work should be done there.

Eating, drinking, and the use of cosmetics in the laboratory should be forbidden. Radioactive materials should not be place in milk bottles, soft drink bottles, or other vessels that might be inadvertently used for food purposes.

Record should be kept of radioactivity brought into the building and of its disposition.

The Laboratory Worker is Surrounded by Many Dangers such as from..........

Handling of infectious materials.

Handling of broken glassware.

Accidental spill of corrosive reagents.

Swallowing of corrosive reagents such as concentrated Sulphuric acid,

Hydrochloric acid, sodium hydroxide, trichloroacetic acid etc.

Swallowing of infectious specimen.

Inhalation of poisonous fumes.

Infection in the conduct of necropsies.

Potential hazards in the form of inflammable chemicals and gas leakages.

Introduction
According to the U.S. Food and Drug Administration (FDA), Good Laboratory Practices (GLP) is a quality system that ensures the nonclinical safety studies are appropriately designed, conducted, and documented to generate reliable data. The laboratory techniques are defined as the processes and practices that are recommended for equipment use in the laboratory, while laboratory methods are based on established scientific principles involving biology, chemistry, and physics, and encompass all aspects of the clinical laboratory from testing the amount of cholesterol in blood to analysing DNA to growing microscopic organisms that may be causing an infection. One of the most significant parts of laboratory techniques and laboratory methods is the proper validation of the equipments (old or new equipments) as well as all the testing procedures/ methods (established or newly developed methods). Analytical method validation is a critical process to ensure the quality and safety of products. The objective of the validation of an analytical method is to demonstrate that it is suitable for its intended purpose; the results are authentic and universally acceptable. An analytical method is suitably well considered when the results generated through different method are consistent, reproducible, and thus, reliable.
The validation is required when
• A new method has been developed
• Revision of established method
• Use of established methods in different laboratories and different analysts, etc.
• Comparison of methods required
• When quality control indicates method changes

Haemostasis refers to the physiological process that prevents blood loss following vascular injury while maintaining fluid blood flow within the vascular system. It involves a complex interplay between the vascular endothelium, platelets, coagulation factors, and fibrinolytic proteins. In animals, laboratory tests for haemostasis play a critical role in diagnosing bleeding disorders, monitoring treatment, and investigating hypercoagulable states. This article discusses the principles, applications, and limitations of laboratory tests used to assess haemostasis in animals.
In veterinary medicine, laboratory tests for haemostasis are essential diagnostic tools for identifying bleeding disorders, hypercoagulable states, and monitoring therapeutic interventions. They are crucial in both emergency and routine clinical settings for animals. These tests provide insights into the functionality of primary haemostasis (platelet plug formation), secondary haemostasis (coagulation cascade and fibrin clot formation), and tertiary haemostasis (fibrinolysis and clot breakdown).

2.1 Beakers

Beakers are the one of most important vessels used in laboratory which is made up of heat resistant glass.

They are available in various capacities ranging from 10 to 3000 mL.

For convenience of pouring solution, spout is provided in the beaker.

They are used to perform titrations & for boiling solutions, etc.,

Beakers are constructed in tall and low forms.

Labour economics : The aspects of economics concerned with the supply and demand for labor. This includes factors affecting the participation rate, wage bargaining and organized labor, training, hours and conditions of work, practices concerning hiring, redundancy, labor turnover, migration and the age of retirement.

Labour intensive : A production process that involves comparatively large amounts of labour; the opposite of capital intensive.

Labour market flexibility : A flexible labour market is one in which it is easy and inexpensive for firms to vary the amount of labour they use, including by changing the hours worked by each employee and by changing the number of employees. This often means minimal regulation of the terms of employment (no minimum wage, say) and weak (or no) trade unions. Such flexibility is characterised by its opponents as giving firms all the power, allowing them to fire employees at a moment’s notice and leaving workers feeling insecure.

Labour theory of value : The notion that the value of any good or service depends on how much labour it uses up. First suggested by adam smith, it took a central place in the philosophy of karl marx. Some neo-classical economists disagreed with this theory, arguing that the price of something was independent of how much labour went into producing it and was instead determined solely by supply and demand.

In economics, labour is defined as manual or mental work for acquiring income for their livelihoods. In farming domain, the agriculture labor is defined as labourers who earn their main source of income by working in farms of others for a wage. In this type, labours may classified in three namely skilled, semi skilled and unskilled labours based on their skill sets.

Skilled labour is a type of labour, who is having specific skills like carpentry, black smiths, drivers, masonry etc will be utilized to perform a specific function, and correspondingly their wages will be on the higher side than other two types. 

6.1 Introduction

This chapter describes the development of data logger of agricultural field parameter monitoring system with LabVIEW. The system comprises of two sections- Fields device and data logger. Field device comprises of Arduino, smoke detector (for sensing hazardous gases), soil moisture sensor, temperature sensor, humidity sensor, water level sensor, battery, display unit, 2.4 GHz RF modem. Data logger comprises of Arduino, display unit, 2.4 GHz RF modem, power supply, PC with LabVIEW. The system is designed to monitor the environment and agriculture field parameters. The field device is designed to collect all physical parameters and communicate it to the receiver end with LabVIEW. For establishing communication between two sections 2.4GHz RF modem is used, which can be replaced with any other modem also.Fig.6.1 shows block diagram of field device and Fig.6.2shows block diagram of data logger.Table.6.1 & Table.6.2 shows the component list required to develop the field device and data logger.

Introduction

Enhancing productivity and profitability are two major factors of agricultural growth for the future. Over dependence on monsoon and limited scope for area expansion made agriculture development is critical to generate employment and additional income for the rural poor. Major part of agricultural population is small and marginal farmers who are economically vulnerable and poor. The consequence of inclement weather on crop production affects ultimately livelihoods of farmers. In the changing scenario improvements in farmers’ productivity and profitability, diversification of agriculture have been acknowledged to enhance profit, generate additional employment for rural masses and to conserve the natural resources.

Integration of different component at farm level is the need of hour to enhance the profitability of agriculture production system. Jharkhand state a resource rich state wheremonocroping in rainfed agriculture with small undulated upland is in practice. Rural economy is mixed type where forest produce particularly lac play important role. To enhance rural economy, integration of agriculture with forest based intervention appears to be viable option to enhance profits and productivity of upland which is almost sixty percent of total cultivable land. Even though moisture is available during Rabi free grazing made imperative cultivation of second crop. Continuous aberrant weather demand the inclusion of forest resources to be exploited in diversified and integrated manner as forest interventions require little water.