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Marigold is one of the easiest annual flowers to cultivate, having wide adaptability. Flowers are attractive, have good keeping quality with a wide spectrum of colour, shape and size. They produce marketable flowers in a short time and are used widely for religious and social functions as well as for garden display. 

Importance and Uses

 Marigolds are sold as loose flowers, as cut flowers and widely used for various decorations and garland making. They are also excellent landscape plants widely used for bedding purpose, in herbaceous borders and can also be grown in pots. French marigolds, being dwarf, are suitable for hanging baskets, rockeries, edging and window boxes. In fact, marigolds rank next only to jasmine in popularity in South India 

Bud caterpillars : Helicoverpa armigera : Noctuidae

Eggs are laid singly on young buds.  Larvae feed on developing flowers by damaging florets. They can also eat the leaves.

Leaf webber : Phycita sp. : Phycitidae

Larvae of Phycita sp. feed on heads of buds and flowers.

Introduction

Marigold is an important flower crop in India. They are extensively used for making garlands, beautification and other purposes i.e. oil extraction and therapeutic uses. Apart from these uses marigold is a widely grown plant in gardens and for pots. Marigolds are also grown commercially for extraction of carotene pigments mainly xanthophylls. The carotenoid extracted from petals are added to poultry feed for intensification of yellow colour of egg yolk. Lutein which is the major constituent of xanthophylls is used for colouring food stuffs.

Marigold plantation has been found beneficial to reduce the population of nematodes. Root-knot (Meloidogyne spp.) can be effectively managed in the field without pesticides.

Genetics

Marigold belongs to family Asteraceae. There are about 33 species of the genus Tagetes. Important species are T. erecta, T. patula, T. tenuifolia, T. lucida, T. lacera, T. lemmonii, T. minuta, T. pusilla and T. corymbosa. The chromosome number in diploid species i.e. T. erecta, T. tenuifolia, T. elongata, T. lemmonii, T. jaliscensis and T. lucida is 2n = 24, however, tetrapoid species i.e. T. patula, T. minuta, T. remotifolia, T. mendocina and T. biflora is 2n = 48.

ABSTRACT

The Mumbai region of Maharashtra has been witnessing very rapid urbanization development in terms of industrial, commercial and residential establishments. This has resulted in reclamation of tidal flats and estuarine regions. The ‘marine clay', characteristic of this environment has high swelling potential and consequently affects the performance of the structures. This will have a serious negative impact on the urbanization expansion. In this study, an attempt is made to map the spatial distribution of these expansive clays using the state of art Hyperion data. Reflectance spectra of representative samples collected from the field is used to map marine clays using Spectral Angle Mapper (SAM) technique. Laboratory analyses of these foundation soils (reflectance spectroscopy, mineralogy and Atterberg limits) indicate that they have significant amount of montmorrilonite and has good swelling potential (A_C>1.4). The derived expansive clay abundance map will be of use while planning appropriate remedial measure and future development.

Key Words : Swelling clay, Spectral characters, Geotechnical properties

1. I Kilo joule - 10 joule.

2. 1 Mega joule -10º joule.

3. 1 Kilowatt hour-3.6*10 joule.

4. The heat engine converts heat energy into mechanical energy.

5. The machine which converts one forms of energy to another form is called as engine or prime mover.

6. Fahrenheit scale is called as British thermal unit and denoted by B.Th.U.

7. Internal combustion engines using coal gas as fuel was made by Frenchman Etienne Lenoir (1860).

8. Silent gas engine was developed by Germans Nileolansotto & Eugen Langen.

9. Rudolph diesel made the first diesel engine in 1894.

10. TDC is also called as inner dead centre.

The resources on the land have started showing sign of faster depletion owing to the population explosion, over exploitation of mineral and water resources and also the improper exploitation and the related wasting of the natural resources. Hence a time has come to search for more and more newer natural resources amongst which the ocean resources gain supreme importance as this treasure is yet to be hunted due to the non - availability of suitable technology for the same.

Such ocean resources mainly include the mineralogical and biological resources. The ocean biological resources, their birth, growth, population and migration greatly depend as chlorophyll content, total dissolved oxygen, total suspended solids, salinity etc. These parameters which constitute the marine environment drastically change in a quick span of time owing to the dynamically changing marine forces.

Abstract

GIS in marine fisheries has taken off much later than its application in land. But the strides made in this field since 1990’s has been quite significant, as many serious mapping studies and modeling initiatives have been recorded. With the third dimension added to the 2D spatial data, the marine GIS have all the ingredients of making it a fertile domain for research for decades to come. Most of the studies and products pertaining to GIS in fishery management had been revolving around mapping of habitats, geo-referencing of catch and effort data and modeling linking oceanographic and biogeochemical factors with living resources. Satellite remotely sensed information like ocean colour can act as a surrogate to fish biomass. Further remotely sensed SST, wind and other parameters can be effectively modeled to predict fish abundance, their habitat and related aspects. This manuscript pours into the various products and outputs based on GIS in marine fisheries around the world and the Indian initiatives.

Key words: GIS, marine resources, oceanography, primary productivity and climate change

1.0 Introduction

Geographic Information Systems (GIS) have been one of the most prolific scientific enhancements in recent past, which by virtue of out of box expansion of traditional database concept have opened up an entirely new vista of knowledge and opportunities. It’s probably one such unique application where information collation and analysis happen hand in hand resulting in palpable mining of data in perspective. A formal introduction to GIS would define it as a collection of computer hardware, software, data and personnel designed to collect, store, update, manipulate, analyze and display geographically referenced information. By virtue of its conceptualizations, there are no single GIS; systems can be assembled in an infinite number of ways. This is one such field, which probably always seeks the frontiers of innovations in the fields of ICT and modeling.

Abstract
Kerala with its extensive coastline and rich marine resources, plays a pivotal role in India’s fisheries sector. This paper delves into the multifaceted dimensions of marine fisheries in Kerala, highlighting its economic, environmental, and social significance. Kerala boasts an exclusive economic zone (EEZ) spanning 2.19 lakh sq km and a coastline spanning 590 km. Its marine waters, especially in the Arabian Sea, are highly productive. The paper explores the recent advancements and achievements in Kerala’s fisheries sector, driven by mechanization and motorization initiatives. In 2022, Kerala recorded its highest marine fish catch of the decade, totalling 6.87 lakh tonnes, marking a 24% increase from the previous year. Key species such as oil sardine and Indian mackerel demonstrated significant recoveries, underscoring the sector’s resilience and adaptive capacity.
Sector-wise analysis reveals that pelagic finfishes dominate the landings, with oil sardine and Indian mackerel leading the pack. Demersal and crustacean landings also contribute significantly to the state’s fishery output. The abstract discusses the distribution of catch among different districts, with Ernakulam emerging as a major contributor. Furthermore, the socio-economic impact of marine fisheries is examined, highlighting its role in livelihoods for approximately 8 lakh people across 222 fishing villages. The sector not only provides income but also supports cultural identities and community cohesion along Kerala’s coastal areas.
Keywords: Marine resources, Production, Kerala, Landing and Fisheries sector.

ABSTRACT

The world ocean with a coastline of approximately 3,50,000 km is the largest ecosystem on earth. Marine organisms are the major, sustaining components of ecosystem processes and are responsible for biogeochemical reactions that drive our climate changes. Despite this, many marine organisms are poorly described and little is known of broad spatial and temporal scale trends in their abundance and distribution. With new molecular and analytical techniques we can advance our knowledge of marine biodiversity at the species level to understand how marine biodiversity supports ecosystem structure, dynamics and resilience. We can then interpret environmental, ecological and evolutionary processes controlling and structuring marine ecosystem biodiversity. In biotechnology applications marine microbes represent a potential source for numerous commercially important biomolecules and their bioremediation capabilities are also remarkable. They also play a crucial role in decomposition of organic matter and cycling of nutrients. Microbes also serve as food for some bottom-living organisms. Understanding and preserving biodiversity was one of the most important global challenges for the past 20 years and will continue to be an important scientific issue into the new millennium. Our knowledge of marine microbial diversity has, however, been severely limited by relying on microorganisms that have been cultured which are less than 1%.

Introduction
Marine Pollution
The pollution of the sea substances that humans have directly or indirectly introducedinto the marine environment, which can have negative impacts on the ecosystem, including health risks to people, obstructions to marine activities, and a decline in the quality of seawater.
It happens when invading species or chemicals or particles or garbage from industry, agriculture, or residential sources find their way into the ocean and can have detrimental impacts.
The majority of maritime pollution originates on land. Nonpoint causes of pollution include wind-blown debris and agricultural runoff.
A great deal of potentially harmful substances stick to small particles, which plankton and benthos animals (most of which are deposit or filter feeders) then consume.
Pesticides swiftly find their way into marine food webs after entering the marine ecology. These pesticides can induce diseases and mutations once they enter food webs, which can be dangerous for both humans and the entire food chain.
Marine food webs may also be exposed to toxic metals. These have the power to alter biochemistry, behavior, reproduction, tissue composition, and growth inhibition in marine organisms. Additionally, a lot of animal diets contain a lot of fish meal or fish hydrolysate. Toxins from the sea can thus spread to land animals and then show up in meat and dairy products.

Increasing pressures exerted on the environment by humans make protection of natural areas crucial for the preservation of biological diversity. Protected areas are one of the most effective tools available for conservation of biodiversity.

The IUCN defines a Protected Area as ‘an area of land and/or sea specially dedicated to the protection and maintenance of biological diversity, and of natural and associated cultured resources, and managed through legal or other effective means.

Since 1986, the IUCN commission on National Parks and Protected Areas (CNPPA) has been promoting the establishment and management of a global representative system of Marine Protected Areas (MPAs) which play a critical role in the conservation of biodiversity. MPAs are growing in importance globally as practical and potentially effective options for the management of fisheries, the protection of biodiversity and the generation of income from eco-tourism. The effective management of MPAs to ensure that they meet their declared objectives poses many challenges.

The IUCN has defined an Marine Protected Area (MPA) as ‘Any area of the intertidal or subtidal terrain, together with its overlying water and associated flora, fauna, historical and cultural features, which has been reserved by law or other effective means to protect part or all the enclosed environment (Kelleher,1999).

Abstract
The oceans, which make up more than 70% of Earth’s surface, are home to a vast variety of marine ecosystem services that sustain human civilization, health, and the economy. The 8.06% percent of the ocean covered by marine protected areas with an around of 18,200 marine protected areas with a total area about 29,202,667km2. A novel, pertinent, and science-driven framework for classifying regions and connecting their effects on people and the environment is the marine protected area system. Marine protected areas create high-quality metrics to augment the official World database on protected areas reporting by utilizing science-based frameworks to evaluate the degree of protection and stage of setup throughout the world. This article presents the most recent official coverage statistics for marine protected areas.
Keywords: Marine Protected areas, Ecosystem, Protection and International
Distribution of Marine Protected Areas
There are 8.06% marine protected areas (MPAs) in the world. The Global Ocean can be divided into areas within national jurisdiction (National Waters) and those in international waters (Areas Beyond National Jurisdiction (ABNJ)). Governments in national waters with established legislative frameworks find it easier to establish MPAs. MPA creation is more challenging in ABNJ because of the intricate legislative system that governs the state.
As a result, compared to ABNJ, a significantly larger number of MPAs have been established within national waters. 39% of the world’s ocean is made up of national waters, of which 18.43% are currently protected areas.

Introduction

The traditional approach to transferring genes from wild to cultivated species is based on interspecific hybridization followed by selection of hybrids that combine the trait with the cultivated genetic background. This breeding strategy is achieved by various backcross generations in which the selected hybrids at each generation are crossed back to the cultivated genotype with the aim of reducing the wild genome and its undesirable traits. The use of molecular markers has allowed this breeding approach to be greatly improved, since these markers directly reveal genetic variability through DNA analysis (Staub et al. 1996), and therefore their detection is not influenced by environmental effects. Since the development of numerous molecular markers for plant genometatic analysis, the possibility to select the genotype instead of the phenotype has been closely examined, leading to the concept of molecular marker-assisted selection (MAS; Paterson et al. 1991). The basic concept of MAS for crop improvement has been well discussed by Collard et al. (2005) which is most appropriate for this chapter. The most widely used markers suitable for MAS are RFLP, RAPD, AFLP, and SSR. Their common origin is point mutation or chromosome rearrangements that were accumulated during the evolution of the species without negatively influencing their survival and reproduction. The choice of the most suitable markers for MAS can differ and depends on the labor required for their detection, possibility of revealing single or multiple loci, dominant or co dominant nature and costs.

Marker Assisted Selection (MAS)

Marker Assisted Selection [MAS] is defining as the indirect selection for a desired plant phenotype based on the banding pattern of linked molecular (DNA) markers. It is based to infer the presence of a gene from the presence of a marker which is tightly linked to the gene of interest and high heritability of the gene of interest. Marker assisted selection (MAS) is also known by other names as marker aided selection and marker assisted breeding (MAB).

25.1 Introduction

The method of selection in which a gene/QTL (Quantitative Trait Locus) is selected on the basis of the genotype of the molecular marker (also called DNA marker) linked to this gene/QTL is known as marker-assisted selection (also called marker-aided selection). The idea of marker-assisted selection (MAS) was first advocated by Tanksley (1983). MAS can be used in combination with the conventional breeding methods or novel breeding schemes could be devised to take full advantage of the marker data. MAS has been widely used for introgression of the desired genes and QTLs using backcross programmes.

25.2 Marker-Assisted Backcrossing (MABC)

The backcross programme based on molecular marker is usually called marker-assisted backcrossing (MABC). MABC has three activities: (a) foreground selection, (b) background selection and (c) recombinant selection.

25.2.1 Foreground Selection

The use of molecular marker to select the gene/QTL being introgressed is known as foreground selection. The objective of foreground selection is to select the target gene, i.e., the gene being introgressed, without phenotypic evaluation for the trait governed by the target gene. Foreground selection for a gene/QTL will be highly desirable in a variety of situation, some of which are listed below:

ABSTRACT

In last twenty years, molecular biology has revolutionized conventional plant breeding techniques in many areas. The use of molecular markers in conventional breeding techniques has also improved the accuracy of selection and allowed breeders to produce varieties with combined traits that were difficult before the advent of DNA technology. Among vegetables, onion (Allium cepa L.) is the most important cash crop of India. Onion productivity in India is much lower (11.38 tones / ha.) compared to other countries (44.1 tones / ha. in Japan). Purple blotch disease (PBD) caused by Alternaria porri (Ellis) Ciferri decreases the yields by up to 50 percent. Genetically resistant onion varieties are the most economical, simple and eco-friendly mode of disease management. The advent of mo- lecular markers provided the opportunity to understand the genetic resistance more pre- cisely. In this paper we present our successful efforts in developing PCR based markers linked to purple blotch disease resistance, so that such markers can be easily integrated into PBD resistance breeding program in onion. Onion germplasm was screened for PBD resistance. Line PBR 287 was identified as a good source of resistance and the genetics of inheritance of resistance in this line was worked out. The protocols for isolating good quality DNA and PCR amplification conditions were standardized. 37 polymorphic mark- ers were identified between resistant and susceptible parents. Crosses were made between resistant and susceptible parents. Parents, F₂ and individual F₂ progenies were subjected for analysis of genetics of resistance and RAPD marker analysis. F₂ individuals segregated in 3:1 ratio for resistance. DNA from resistant and susceptible F₂ individuals was pooled separately for Bulked Segregant Analysis (BSA). BSA identified one RAPD marker of 900bp being linked to resistance. Further Chi-square test for detecting the deviation of segregation of this 900bp marker confirmed segregation with classical Mendalian ratio 3:1 for a monogenic trait. Identified marker is of great practical utility to the plant breeders to screen large number of populations for purple blotch disease resistance at the early stages of the crop even in absence of the pathogen.

ABSTRACT

During the efficient genetic transformation of plants with the gene of interest, some selectable marker genes are also used in order to identify the transgenic plant cells or tissues. Antibiotic and herbicide selective agents and their resistance gene are commonly used to introduce economically valuable genes into crop plants. According to the biosafety authority and consumer view points, the presence of selectable marker genes in released transgenic crops may be transferred to weeds or pathogenic microorganism in the gastrointestinal tract or soil, and will make them resistant to treatment with herbicides or antibiotics, respectively. Sexual crossing also raises the problem of transgene expression because redundancy of transgenes in the genome may trigger homology-dependent gene silencing. The future potential of transgenic technologies for crop improvement depends greatly on our abilities to engineer stable expression of multiple transgenic traits in a predictable fashion and to prevent the transfer of undesirable transgenic material to non-transgenic crops and related species. Therefore the development of efficient marker free transgenic plants system is very essential. That is why many technologies and various approaches designed to timely elimination of transgenes when their function is no longer needed. Due to the limited number of available selectable marker genes, in future the stacking of transgenes will be increasingly desirable. The production of marker-free transgenic plants is now a critical requisite for their commercial deployment and also for engineering multiple and complex trait. Here we describe the current technologies to eliminate the selectable marker genes (SMG) in order to develop marker-free transgenic plants and also discuss the regulation and biosafety concern of genetically modified (GM) crops.

Introduction

The main goal of plant breeding is to increase crop yield and the second goal is to improve quality, develop light- and heat-insensitive varieties, tolerance to biotic and abiotic stresses, simultaneous maturation, and water and nutrient use. Efficiency, elimination of toxins, and cultivation of different product groups allow to achieve high agricultural yields and sustainable development. Better understanding and development of genetics has increased the efficiency of plant breeding to achieve desired goals in agricultural crops. Effective and efficient use of molecular markers in crop improvement programs increases selection efficiency and productivity and accelerates the growth cycle to create new varieties with good results. Marker assisted selection (MAS) is a technique that uses markers (morphological, biochemical, or DNA/RNA variation-based markers) to indirectly select for traits of interest (e.g., productivity, disease, genetic determinants resistance, abiotic stress tolerance and/or quality). This process is used in plant and animal breeding. The rapid development of biotechnology has allowed breeders to develop better selections to replace traditional selections based on phenotypic pedigree.

1. Markers: Easily identifiable features are called markers or marker features.

2. Types of marker

These markers are three types

2.1.Morphological markers: These relate to the size, shape, color and surface area of different parts of the plant.

2.2.Biochemical markers: Such markers refer to changes in protein structure. For example, isozymes and storage proteins.