eChapters

Antimicrobial drugs are important medicines which are continuously developed and evolveddue to the phenomenon of development of antimicrobial resistance to antimicrobial agents by microorganisms since the discovery of Penicillin by Sir Alexander Fleming in 1928-29. It has become essential for both pharmacologists and clinicians to optimize the certain principles of pharmacokinetic-pharmacodynamic aspects in both development of new antimicrobial drugs and in getting better therapeutic outcome in actual clinical situations in the field conditions to overcome the challenge thrown by microorganisms to the mankind.

Pharmacokinetics (PK) describes the relationship between the dose of any drug given and the resulting concentration in the body. It includes the physiological process of absorption, distribution, metabolism and elimination whereas Pharmacodynamics (PD) describes the interaction between the drug concentration and pharmacological/therapeutic effect in vivo. It is determined as a definite concentration of a drugand its ability to kill or inhibit the cultured pathogenic microorganismin vitro that is aptly described asminimum inhibitory concentration (MIC) in case of antimicrobial drugs.

The integration of pharmacology and bioinformatics has ushered in a new era of drug discovery and therapeutic innovati on, particularly in veterinary medicine. This interdisciplinary approach leverages computational tools to enhance the identification of drug targets, optimize treatment strategies, and address challenges such as antimicrobial resistance (AMR) and species specific drug metabolism. Pharmacogenomics, one of the most promising applications, allows for personalized medicine by tailoring drug therapies based on genetic variations in animals. Additionally, bioinformatics is revolutionizing vaccine development by identifying conserved antigens, aiding in the design of broad-spectrum and species-specific vaccines. In silico screening accelerates drug discovery by predicting drug-target interactions, reducing the time and cost of developing new veterinary therapies. Predictive modeling enables the simulation of disease progression and treatment outcomes, improving disease management. As bioinformatics technologies advance, they are poised to transform veterinary pharmacology, offering more precise and effective therapeutic options. This paper explores the synergies and applications of integrating pharmacology with bioinformatics to improve veterinary therapeutics, combat AMR, and accelerate drug discovery.

Post-traumatic bleeding, caused by accidents or battlefield injuries, is a major worldwide issue due to the associated high fatality rate. Significant efforts have been made to develop hemostatic medications that help reduce post-traumatic bleeding. The use of naturally abundant and highly biocompatible polymers, such as alginate, can have a substantial impact on the efficacy, safety and cost effectiveness of hemostatic medicines.We haveexaminedthe effectiveness of developed hemostatic hydrogel (SA-CZ) containing calcium (Ca2+) and zinc (Zn2+) in treating low-pressure bleeding wounds with the added benefit of promoting faster wound healing. The hydrogel nature of the hemostatic formulation poses limitation as hemostatic material due to its fluid absorption capability. Therefore, we converted the formulation in the form of dry formulation. Further, we explored the use of dry hemostatic particles (SA-CZ DHP) made from sodium alginate supplemented with Ca2+ and Zn2+ to manage excessive blood loss after post-traumatic hemorrhage.SA-CZ DHP showed substantial in-vitro efficacy, as observed by the significant reduction in coagulation time with better blood coagulation index (BCI) and no evident hemolysis in human blood. SA CZ also showed enhanced cellular migration (1.58-fold) in-vitroand improved wound closure (≈70%) as compared with betadine (≈38%) at the 7th-day post-wound creation in-vivo (p<0.005).The safe SA-CZ DHP showed high absorption and accelerated blood clotting kineticswith reduced coagulation time (≈70%, p<0.0001) in whole human blood. SA-CZ DHP significantlyreduced the mean blood loss (≈90% in SD rats tail incision), and bleeding time (≈60%in BALB/c mice tail incision) was at par with commercially available Celox™hemostatic granules.

The Indian subcontinent has a vast costal belt along with the wide range of forest environment, which host large number of unexplored plant/marine species. This diversity has been the source of unique chemical compounds with the potential for industrial development as pharmaceuticals, cosmetics, nutritional supplements, molecular probes, fine chemicals and agrochemicals. In recent years, a significant number of novel metabolites with potent pharmacological properties have been discovered from the natural sources including marine and terrestrial plants. Recent move of society towards nature for the treatment of various diseases where there is no satisfactory cure in modern medicine has diverted the attention of natural/medicinal chemists and biologists to unravel their chemical characteristics and biological activities together in order to define their therapeutic potential in the light of modern pathobiological understandings. This move has led collectively to rediscover, design and refine the therapeutic application of medicinal plants/marine sources.

GPCRs are integral membrane proteins that contain seven transmembrane (TM) α-helices, with a short cytoplasmic Helix 8 interconnected to the TM7 and the cytoplasmic C terminal. The amino terminal is extracellularly disposed.

The 7 TM helices are interconnected by 3 extracellular and 3 intracellular loops. On the extracellular side between TM3 and TM4 is located a conserved disulphide bond. GPCRs being allosteric proteins, agonist binding at one site that is accessed from the extracellular space and termed the orthosteric site, promotes allosteric binding to another partner, a heterotrimeric G protein at the cytoplasmic side. Agonist binding not only promotes binding of the receptor to a G protein, but the affinity of the agonist for the receptor is increased by the G protein.

Use of Antimicrobial Agents

The most frequently used classes of antimicrobial drugs in animals include tetracyclines, sulfonamides alone or in combination with potentiators, quinolones particularly fluoroquinolones, aminopenicillins alone as well as in combination with potentiators, first- and second-generation cephalosporins and also third- and fourth-generation cephalosporins, macrolides, and glycopeptides [1]. The “Terrestrial Animal Code”, issued by the Office International des Epizooties (OIE) in 2022 represents recent guidance for the responsible and prudent use of antimicrobials in veterinary medicine [2]. It describes the respective responsibilities of the competent authority and stakeholders in all processes pertaining to veterinary antimicrobial agents ranging from authorization to the administration to the animals.

Artificial Intelligence (AI), Machine Learning and Data Analytics seem to be invading all the fields to bring in efficiencies in those respective fields. However, Toxicology field seems to be lagging behind in these areas although there is a huge scope to use these advanced technologies.

AI, data analytics and machine learning can be used for creating toxicology databases on chemicals, predictive modelling and AI for risk assessment, amongst other applications. One such effort was carried out for use of AI in creating a BOT, a ToxBot, for conducting risk assessments on chemicals which helped in gaining lot of efficiencies. The ToxBotwhich was created helped in conducting risk assessments and reducing time for such risk assessments from48 hours to 15 minutes. This also ensured using a uniform database, consistent output and helping toxicologists in arriving at unform conclusions. To begin with, the sponsors need to be very clear about the objective and expected outcome of the AI solution. The first step in creating these AI solutions is creation of a datasbase. While, creating AI solutions for risk assessment two aspects are important- source of Toxicology thresholds and the other one to derive exposure to population to assess safety. Creating a Toxicology data base for deriving thresholds is tedious. Toxicology studies generate huge data on multiple chemicals, drugs and botanicals. However, these data are scattered, not uniformly documented, and there is lack of efforts to compile them. Each constituent has data on each end point and these data are spread across multiple species. Hence the Toxicology data on each constituent has variables such as species, routes, doses, duration and end points. The efforts start with creating a complete Toxicological profile including all available data on each end point in each species by specific route of administration. These profiles then form the basis of a data base, which then enable to derive safety thresholds. The other database or system is to derive the exposure. This depends on the type of product, daily dose, frequency, route, concentration of chemical and the target population. These can be populated through an exposure matrix. For Al, these data need to be documented in uniform manner for subjecting it to Machine Learning and creating AI solutions. The AI experts would then be required to understand the entire process of the desired outcome and how the dataare used, at what points. This helps them in creating an algorithm. Once the algorithm and coding is created, the product is ready to be tested.

Buffalo farming offers a sustainable and economically viable solution to meet the growing demand for safe food production, particularly in regions with rising food insecurity. Buffaloes contribute around 45% of India’s total milk production and are more efficient in converting low-quality feed into high quality milk and meat, making them ideal for marginal farming environments. Buffalo milk, which has higher fat, protein, and mineral content than cow milk, is in demand for value-added dairy products, such as cheese, and fetches higher market prices. This enhances farmers’ incomes, especially small-scale dairy farmers, by ensuring higher returns on investment. Additionally, buffalo husbandry mitigates risks in crop failure due to climate change and provides sustainable employment opportunities. Furthermore, buffaloes produce less greenhouse gas emissions per unit of meat compared to cattle, making them more environmentally sustainable and climate smart. By advancing breeding practices and adopting scientific management, buffalo farming has the potential to drive the future of safe and sustainable food production, contributing significantly to food security and rural development.

Introduction

Antimicrobial resistance (AMR) represents a critical public health crisis, with far-reaching implications for human, animal, and environmental health. If unchecked, by 2050, the global toll of AMR-related deaths could exceed 10 million, with Asia and Africa alone projected to account for approximately 4 million fatalities. In a potential ‘superbug’ outbreak scenario, the economic loss could reach as high as USD 100 trillion, especially impacting low- and middle income countries (LMICs) like India (O’Neill, 2014). AMR complicates the treatment of a wide array of infections, transforming previously manageable conditions into fatal threats. In 2019 alone, AMR was directly responsible for approximately 1.27 million deaths and contributed to an additional 5 million fatalities—surpassing the mortality rates of major infectious diseases such as HIV/AIDS and malaria (Murray et al., 2022). The urgency of addressing AMR is underscored by a World Bank report, which warns that unchecked AMR could cause a global GDP decline of 1.1% to 3.8% by 2050, and averting this scenario may require an estimated annual investment of around 9 billion USD (Aslam et al., 2024).

Introduction

Discovery of the antimicrobial agents in the early twentieth century was a breakthrough to safeguard the public health from the microbial infection. Propensity of these compounds to kill pathogens influenced the discovery of novel antimicrobials. However, since the 1970s, soon after the discovery of the fluoroquinolones, no major antibiotics have been introduced. The prevalent issue is largely attributed to the repercussions of antibiotic overuse or irresponsible utilization across diverse contexts, predominantly in clinical treatment, agricultural practices, animal healthcare, war crisis and the food system. Moreover, the accessibility and availability of antibiotics results in antimicrobial resistance (AMR). Nonprudent use of antibiotics accompanied by genetic plasticity allows microorganisms to adapt to the effect of antimicrobials causing a rapid surge of antimicrobial resistance. Extensive use of antimicrobials in animal husbandry is a major driver of AMR selection and the emergence of antimicrobial-resistant bacteria (ARBs) (World Health Organization, 2014).

This study aimed to optimize the incorporation levels of whole egg powder (WEP) and skim milk powder (SMP) and to evaluate the storage stability of the resulting protein-rich pasta. Response Surface Methodology (RSM) with a Central Composite Design was employed for optimization; thirteen formulation combinations were tested. The best formulation was identified as 5% WEP and 10% SMP. This optimal mix resulted in a significant improvement in the proximate composition of the fortified pasta compared to the control. Both the control and fortified pasta were stored under aerobic conditions at 20-30±5°C in aluminium foil-LDPE laminate pouches for 60 days. The pasta samples were analyzed at intervals of 1, 15, 30, 45, and 60 days. Storage studies revealed an increase in TBARS, FFA, peroxide values, and microbial counts in both pasta types, but the control samples exhibited a significantly lower rate of increase (p<0.05). Sensory evaluation showed lower scores for the fortified pasta compared to the control. Despite these findings, the fortified pasta with 5% WEP and 10% SMP maintained its physicochemical, colour, textural, microbiological, and sensory qualities over the 60-day storage period. Microstructure studies revealed increased interaction between added protein (WEP & SMP) and starch molecules, indicating a potential option to produce high quality pasta with enhanced nutritional and functional properties, thereby enhancing the practical implications of the research.

With the recent advances in cognitive science and applied ethology, it become imperative to assess the effect of various negative stimuli on the animal welfare and meat quality in goats during slaughter. The present study was designed to assess the effect of exposure of goats to slaughter ambient such as the smell of blood, vocalization, noise, movement of equipment, and unfamiliar workers could initiate stress responses, thereby affecting animal welfare and meat quality. Boer cross bucks (age 8-12 months of age, 25-30 kg live weight, n=18) were halal slaughtered into three groups viz., Control (slaughtered alone), E (exposed to act of slaughter), and S (slaughtered in front of E). The vocalization and avoidance behavior were present in 50% of goats upon exposure to slaughter ambient. The time to death and bleed out percentage did not differ significantly among treatments. The exposure of the goats to the slaughter resulted in a significant (p<0.05) increase in the nor-adrenaline, and β-endorphin. Based on the present study, it can be concluded that exposure to slaughter ambient had an effect on behavioral responses, carcass, and blood biochemical factors. Thus, the present study highlighted the issue of emotional stress and slaughter empathy in goats. These findings could help in harmonizing all practices of slaughter and improving animal welfare while fulfilling religious and customary demands.

Species determination in meat products is crucial for traceability and preventing fraud, especially in the buffalo meat industry, which faces significant price disparities across regions. The complex global trade networks, with many intermediaries and processing stages, increase the risk of obscured provenance and adulteration. This not only poses serious public health risks but also leads to economic losses. Thus, there is an urgent need for rapid, reliable testing methods to verify buffalo meat authenticity and detect mislabeling. Existing protein-based and DNA-based assays, including isothermal and non-isothermal amplification techniques, often lack sensitivity, specificity, practicality, repeatability, and accessibility. The present study introduces a Point-of-Care (POC) polymerase spiral reaction (PSR) assay for rapid, on-site identification of buffalo tissue, especially in resource-limited settings. The PSR method uses chemical-free snap-chill DNA extraction, highly specific mitochondrial d-loop gene primers for amplification, and unique chromogenic indicators for visual detection, overcoming the limitations of current detection methods. The effectiveness of the chromogenic indicators was validated against SYBR Green I and agarose gel electrophoresis methods. The assay showed high specificity, with no cross-amplification in common meats like beef, mutton, chevon, and pork, and sensitivity down to 10 fg of buffalo DNA. It detected buffalo meat adulteration as low as 0.1%. The assay’s accuracy was confirmed through comprehensive validation using diverse, blind, and processed samples, compatibility testing with a commercial DNA isolation kit, and comparison with standard buffalo-specific PCR. This study is the first to report a PSR colorimetric assay identifying buffalo tissue within 90 minutes in resource constrained settings.

The present study was envisaged to develop kodo millet incorporated chicken meat nuggets and to explore the effect of kodo millet flour on the shelf life of chicken meat nuggets. The chicken meat nuggets were developed by using kodo millet flour (KMF) at three different levels (T1-6%, T2-8% and T3-10%) by replacing lean meat. Appearance/colour and flavour score, juiciness and overall palatability were found significantly higher (P < 0.05) for T1. On the basis of sensory scores, control and T1 were selected for further comparison and storage studies in refrigerated temperature for 28 days and assessed for microbial analysis at regular interval of 7 days. Thiobarbituric acid (TBA) and standard plate count (SPC) values increased significantly (P < 0.05) in both variants during storage. These values were found lower in treatment. Results of sensory evaluation studies during storage also revealed decrease in sensory scores in almost all occasions. T1 (treatment containing 6 % KMF) was rated higher for flavour, texture, juiciness and overall palatability throughout the storage period. It was concluded that functional chicken nuggets could be developed by replacing the chicken meat in standardized formulation at the level of 6 % kodo millet flour. The developed product maintains better physiochemical, microbiological and sensory qualities during refrigerated storage and was acceptable up to 14 days of storage.

In the present study, chicken (broiler) meat mince was used as protein sources. Incorporation of chicken meat minceat10, 20, 30, 40 and 50percent levelweretriedbesidesotheringredientswhichwereaddedincontrol.Chicken Biscuits incorporated with 20 per cent CMM were preferred the most on the basis of their sensory evaluation. Asthe chicken incorporated biscuits have not been reported in India and abroad. Majority of biscuits available in market are prepared from cereal (refined wheat flour), which has very low protein content & lack lysine, threonine & tryptophan essential amino acids. The biscuits thus developed can contribute good amount of nutrients especially protein if consumed in place of cereal (refined wheat flour) biscuits. The developed products can be used for feeding programme at institutes and school going children and elderly people, who are more prone to malnutrition particularly due to protein with essential amino acids deficiency. Chicken meat is also rich in threonine, tryptophan and lysine. Thus development of chicken incorporated biscuits using chicken meat and wheat flour would provide a nutritious, convenient and ready to eat food item. With an aim to develop biscuits by incorporating the chicken meat, the present study was planned. It was concluded that the biscuits with 20 per cent chicken meat mince and wheat flour would provide a nutritious with sufficient amount of essential amino acids (lysine, tryptophan and threonine), convenient and ready to eat food item. The cost of production of biscuits with 20 per cent CMM worked out to be Rs. 82.40 per kg.

Ready to eat shami kebabs from goat meat with 25, 50 and 75 % levels of goat tripe were prepared and their quality evaluated. shami kebabs prepared with 100% goat were used as control. Significant (p<0.05) increase was observed for pH, diameter shrinkage, drip loss, protein and fat contents in goat tripe incorporated shami kebabs than control. However, significant (p< 0.05) reverse trends were observed for product yield and moisture content. Sensory evaluation scores for appearance and colour, flavor, tenderness, juiciness, binding and overall acceptability were highest for 25% goat tripe incorporated shami kebabs followed by 50% and 75% goat tripe incorporated shami kebabs. Physico - chemical and sensory evaluation scores of 25% goat tripe incorporated shami kebabs were comparable with control goat meat shami kebabs and the values did not differ significantly between them. Thus, it can be concluded that 75% goat meat and 25% goat tripe can be used for preparation of ready to eat goat meat shami kebabs without affecting quality and acceptability.

Three female goats with age of 3, 4 and 24 month with bodyweight 7, 6 and 15 kg, respectively were presented at Veterinary Clinical Complex, Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-AnusandhanSansthan, Mathura, DUVASU during April-May 2024, with a history of right unilateral swelling below the ear and submandibular region. Based on physical examination, radiography and ultrasonography, swelling was found to be a fluid filled cystic structure. Exploratory puncture of the affected areas revealed colourless transparent fluid. Surgical exploration was done and the cystic structure was identified as Coenurus gaigeriupon parasitological inspection due to the presence of its characteristic four distinctive suckers on the scolices and a rostellum with a double crown of hooks. Coenurus gaigeri is the intermediate stage of Taenia multicepsgaigeri. Following surgical intervention, the animals were treated with praziquantel, antibiotic and an anti-inflammatory medication. By the 7th postoperative day, the animals showed uneventful recovery without any complications.

Introduction

Pig production and marketing have been driven by the growing consumer demand for high quality and low cost animal protein. As a result, organized and industrialpiggery has been rapidly developing globally. Pigs are domesticated in various parts of India, especially in South-Central and North Eastern Region (NER), each place has its own locally adapted pig breed, and most households raise at least one or two pigs each year. Pigs are more valuable alive than dead, acting as efficient converters of kitchen and agricultural scraps into nutrient-rich fertilizer, before becoming pork that could even sometimes be given as a wedding gift, used to curry political or social favor, or eaten as part of local celebrations, especially in NER. The 21stcentury agricultural and dietary changes however represent radical departures from the small holder production system and consolidation of industry is clearly visible in the last two decades. It should be noted that the industrialization of pig production is a relatively recent phenomenon globally. In India, too, the speed and scale of change has been phenomenal, conditioned by policies, investments and the transforming economic system.

Introduction

Global meat production is a crucial component of the global food system, driven by expanding populations, wealth development, and shifting dietary preferences. It involves the rearing of animals such as cattle, poultry, pigs, and sheep, and has experienced rapid growth in recent decades. In 2023, worldwide meat production was estimated to reach approximately 350 million tons per year, with chicken, pork, and beef being the most popular forms. Poultry meat is the most produced and consumed meat globally due to its lower cost and shorter production cycles. Countries like the U.S., Brazil, and China are major poultry producers globally. Pork is highly consumed, especially in Asia, with China being both the largest producer and consumer of pork. By 2050, the global human population is projected to reach 10 billion, presenting a major challenge in ensuring adequate balanced food. The demand for animal-based foods is increasing, and the intensification of animal farming is leading to the exploitation of natural resources and resulting in natural calamities that adversely affect humans, animals, and ecosystems. Meat production fosters sustainable development as it employs and supports over a billion people worldwide, many of whom live in underdeveloped regions. Meat contains essential nutrients such as proteins, vitamins, and minerals, which help prevent nutritional deficiencies and improve health (Headey et al., 2018; Zaharia et al., 2021). The meat production industry can also transform grass and agricultural waste into healthy food for people, thereby promoting food security in a world with limited natural resources (Mottet et al., 2017). However, it is important to note that meat has a much larger environmental and climatic footprint compared to plant-based diets (Poore & Nemecek, 2018).

The present study was carried out on gross anatomical and morphometrical studies of extensor and flexor muscles of digits of forelimb and hind limb in six adults non-descript dogs irrespective of sex. Common digital extensor muscle was originated from the lateral (extensor) epicondyle of the humerus with multiple tendons of insertion whereas lateral digital extensor was originated from the lateral ligament of the elbow joint. Extensor pollicis longus and indicisproprius muscle was present under the lateral digital extensor and superficial to the abductor pollicis longus and originated from the middle third of the dorsolateral border of the ulna. Deep digital flexor muscle was comprised of two heads, flexor hallucis longus, and flexor digitorum longus.The average tensile strength for common digital extensor, lateral digital extensor, extensor pollicis longus and indicisproprius, and abductor pollicis longus muscles were 2.65 ± 0.36 kg, 2.20 ± 0.47 kg, 1.60 ± 0.38 kg and 1.95 ± 0.35 kg respectively. Mean Muscle belly length for common digital extensor, lateral digital extensor, extensor pollicis longus and indicisproprius, and abductor pollicis longus muscles was 8.30 ± 0.51 cm, 6.68 ± 0.45 cm, 4.80 ± 0.68 cm and 8.08 ± 0.73cm respectively. Mean tensile rest strength for the superficial digital flexor, deep digital flexor, interosseous muscle, f lexor pollicis brevis and lumbricales was 7.61 ± 0.56 cm, 8.53 ± 0.51 cm, 2.76 ± 0.47 cm, 0.73 ± 0.13 cm and 0.85 ± 0.17 cm respectively. Mean muscle belly volume of superficial digital flexor, deep digital flexor, interosseous muscle, flexor pollicis brevis, and lumbricales muscles was 6.61 ± 0.72 cm3, 7.84 ± 0.53 cm3, 0.24 ± 0.07 cm3, 0.03 ± 0.01 cm3, 0.02 ± 0.00 cm3 respectively.Mean tendon cross section area for muscles tibialiscranialis, long digital extensor, lateral digital extensor, and extensor digitorum brevis was 0.05 ± 0.00 cm2, 0.06 ± 0.00 cm2, 0.04 ± 0.00 cm2 and 0.04 ± 0.00 cm2 respectively.

Introduction

Equines are a fascinating and diverse group of mammals that have played a significant role in human history and continue to do so today. From the majestic horses used for transportation, sport, and work, to the hardy donkeys and mules used for farming and packing, equines have been an essential part of human societies for thousands of years. Equines are social animals and usually live in groups or herds. They form strong bonds with their herd members and communicate with each other through a range of vocalizations, body language, and scent cues. Equines also engage in playful behaviour, particularly when they are young. They can be seen running, kicking, bucking, and frolicking in the fields as a way of expending energy and developing physical coordination [6].

This is a group of mammals that belong to the family Equidae known for their slender legs, hooves, and long faces. Large, soft and expressive eyes, a velvety muzzle, a forelock adorning a smooth face these are just a few of the attributes of the equine head that beckon horse lovers. The equine head, which accounts for 10% of a horse’s total weight, houses a series of intricate turbinates within the nasal passageways. Turbinates are a network of bones, vessels, and tissue that warms, humidifies, and filters the air the horse inhales. In addition, seven air-filled sinus cavities called the paranasal sinuses sit on each side of the head, above, below, and between the eyes. A young horse starts out with teeth that extend up into the sinuses; with wear and age, the teeth descend into the mouth. Eventually, in a horse’s golden years, the sinuses are mostly empty of teeth.The head also contains a plexus of nerves and blood supply in addition to the eyes, ears, nostrils, lymph nodes, guttural pouches, brain, and more than 30 bones[6,7].

As we delve into the subcellular world, we uncover pivotal insights for advancing our understanding of animal health and disease. Immunoelectron microscopy (IEM) is the same as immunohistochemistry or immunocytochem istry at the light microscopy level. The Power of IEM combines the high-resolution imaging capabilities of electron microscopy with the specificity of immunolabeling. This robust procedure allows us to visualize the precise local ization of proteins and other molecules within cells, providing a detailed cellular architecture and function map. This technique is known as immunogold staining (IGS) or immunogold labelling (De Paul et al., 2012).

Principle of Immunoelectron Microscopy (Iem)

The basic principle of IEM is based on the specific binding of a primary antibody to the target antigen in the sample. A secondary antibody, which is conjugated to colloidal gold particles, is then added. This secondary antibody binds to the primary antibody. The gold particles are electron-dense, meaning they scatter electrons effectively. If viewed under an electron microscope, these particles appear as dark spots, indicating the location of the target antigen. This method demonstrates very specificity and high contrast, making it easier to visualize and study the distribution of proteins at the ultrastructural level (Schwartzbach & Osafune, 2010; Murtey, 2016).

Veterinary anatomy is a cornerstone of veterinary education, essential for diagnosing and treating animals. It imparts knowledge about origin, form, structure and function in animals. Traditionally, veterinary anatomy education has been delivered through lectures, dissections, and practical sessions. Anatomy teaching has undergone significant changes to keep up with advances in technology and to cater for a wide array of student specific learning approaches. In recent time a generalized decline in dissection based veterinary anatomy teaching has been observed due to implementation of new curriculum of veterinary anatomy by VCI, legal and ethical issues, unavailability of ethically sourced cadaver, reduced teaching hour to veterinary anatomy, reluctance of students as well as teacher to handle formalin fixed cadavers and specimens etc. Even with the introduction of modern instructional technology and improved teaching methods, dissection continues to remain a foundation of veterinary anatomy teaching. Conventional methods, while foundational, face significant limitations, including the availability of resources and variability in hands-on experiences. As veterinary education evolves, there is a growing recognition of the potential for disruptive technologies to address these challenges and enhance learning outcomes.

The escalating global demand for livestock products, driven by rapid population growth and rising per capita consumption, presents significant challenges for the agricultural sector (Moreau and Jordan, 2005). Traditional livestock production methods are becoming inadequate to meet this demand sustainably. Biotechnology offers novel solutions to enhance productivity, improve animal health, and ensure environmental sustainability (Schmitt and Henderson, 2005). This review aims to explore the role of biotechnology in livestock, focusing on key innovations and their implications for the future of animal agriculture.

1. Genetic Engineering

Genetic engineering has revolutionized the field of animal science by enabling precise modifications of the livestock genome to enhance desirable traits and reduce undesirable ones.

1.1 Transgenic Technology

A mouse was the first successful transgenic animal followed by pigs, sheep, cattle, and rabbits (Shakweer et al., 2023). Transgenic animals are created by inserting foreign genes that encode specific traits, such as growth hormone genes to enhance growth rates or disease resistance genes to reduce susceptibility to infections (Hammer et al., 1985). For instance, transgenic cattle have been engineered to produce milk with modified protein content (Zhang et al., 2019), improving its nutritional profile for human consumption (Brophy et al., 2003). Further, cattle genetically engineered to express human lysozyme in their milk have shown enhanced antimicrobial properties, making the milk safer for consumption (Moreau and Jordan, 2005). These modifications not only boost productivity but also improve the quality of livestock products, making them healthier and more nutritious.

Research techniques are the various methods and tactics strategically used to collect the materials, record the observations and analyse the data for study. These approaches provide a structured framework for achieving research objectives and validate the findings.

Anatomy is the oldest scientific discipline of the medicine, which deals with the form and structure of the organisms. The first documented scientific dissection on the human body were carried out as early as in the third century BC in Alexandria. At that time anatomists explored anatomy through dissection of animals, primarily on pigs and monkeys. The first written evidence of anatomical terms and rational observations can be found in the Egyptian medical papyri. The work of Andreas Vesalius played a significant role in medical research and education. During the 19th century the research in anatomy increased due to discovery of microscopes which allowed in-depth studies of body tissues and their components. However, in the last 100 years due to technological innovations and the growing understanding of related sciences progress in anatomy took place at a much faster pace. A thorough knowledge of research techniques in anatomy is very important to perform systemic experiments to reach a definite conclusion. The technique should be selected according to the aim and field of research. Here we are going to discuss some of the important gross anatomical techniques.

Forensic science has traditionally been associated with human criminal investigations, but its application to veterinary cases is gaining momentum. Hair is a specialized outgrowth of epidermis, a component of the skin. Hair is chemically stable especially when compared to other physiological materials such as blood, semen or any other f luid as it is strongly resistant to decomposition and this property makes hair a nearly ideal type for physical evidence. However, the forensic identification of animal hair requires different skill sets and competencies to those required for human hair comparisons (Tridico et al. 2014). Hair carry a lot of biological information, their examination is easy and very cost effective. Recently Funes et al. (2023) has reviewed both macro and micro quantitative methods for human hair analysis.

The study of the animal hair could represent a useful tool in veterinary forensic investigation and thus can be helpful in species identification in veterolegal cases, illegal transport of slaughtered animals, poaching or wildlife crime, fraud in textile and fur industry, traceability of predators and identification of their victims (Dahiya and Yadav 2013). The use of hair analysis in forensic science dates back to the 19th century, with early forensic scientists recognizing its potential in criminal investigations. Over time, techniques have evolved from simple visual comparisons to sophisticated microscopic and biochemical analyses.

A diverse microbial population colonizes the sterile mammalian gastrointestinal tract during and after the birth. This organized and long-lasting process of colonization by microorganisms has developed into flourishing relationships creating diverse environment within the host gut (Mazmanian et. al. 2005). There are various factors that affect the optimum colonization of gut microbiome and their normal functioning on various organ systems of the host body directly or indirectly. The gut microbiome comprises of a complex combination of microbes (Mezouar et. al. 2018). The mammalian gastro-intestinal tract harbors this complex ecosystem in equilibrium with the host immune system. The most magnificent feature of this commensal relationship is that the host not only tolerates but has also evolved to require colonization by commensal microorganisms for its own development and health. There are various factors that affect the optimum colonization of gut microbiome and their normal functioning on various organ systems of the host body directly or indirectly. The process of colonization is a two way interaction between the host and the microorganisms.

Introduction

Neuroanatomy research covers a wide range of topics including, gross anatomy of brain and spinal cord, imaging technologies for diagnosis, surgical anatomy, microscopic anatomy on nervous system, ultrastructural details on neurons and neuroglia, research on gene and protein expression patterns using in situ hybridization and immunocytochemistry. Despite many challenges, neuroanatomy in animals remains a crucial area of study for understanding the basic principles of structural organisation and its physiology. This review on the master glands i.e. hypothalamus and hypophysis cerebri, is mainly focused on the histomorphology and ultrastructural details reported in rat, rabbit, sheep, goat, buffalo and camel.

Mastering the Master gland

The hypothalamus (“hypo” meaning below, and “thalamos” meaning nuptial chamber / innermost room) is a major centre of the brain consists of regulatory circuits that controls internal body functions for survival. The hypothalamus is a small, central region of the brain composed of a conglomerate of hypothalamic nuclei and their nerve tracts. The hypothalamic neural connections to the pituitary glands in various mammals have been studied under light microscopic examination of sections of hypothalamus and pituitary gland together intact with infundibular stalk (Paramasivan et al., 2011). The nerve fibres of various hypothalamic nuclei coursed on as a distinct bundle into the infundibular stalk and then continued into the neurohypophysis. Through its connections with pituitary gland, the hypothalamus exerts an enormous influence over the endocrine system and thereby over the general metabolic functions and reproduction (Clemente, 1985). The pituitary gland (hypophysis cerebri) is considered as the master endocrine gland as it plays a critical role in influencing and controlling the activities of other endocrine glands viz. thyroid, adrenal, ovary, testis and pineal. However, the hypophysis itself is under the control of the hypothalamus which actually masters the endocrine function of various hormones released from the master gland i.e. hypophysis cerebri.

In the 19th century, Etienne Geoffroy de Saint Hilaire conducted pioneering studies on prenatal anomalies such as cyclocephaly, anencephaly, and twin monsters, and is credited with coining the term 'teratology'. The physician Josef Warkany is considered the father of experimental teratology. His studies led to the definition of both genetic and environmentally induced structural defects. The occurrence of congenital malformations varies significantly across species, breeds, and geographic locations. Studies suggest that approximately 1.5% to 6% of live-born domestic mammals exhibit recognizable congenital malformations. These malformations range from complex anatomical abnormalities to complicated enzyme defects detectable only through advanced molecular diagnostics. Occurrence of these malformation is very low in cat while in sheep, cattle and horses it is almost 3-4 % and highest in dogs and pigs and is range about 6%. The genesis of congenital malformations is understood as interplay between an embryo's genetic makeup and its developmental environment. Teratology thus highlights the critical periods during embryonic development when external influences can significantly impact normal growth and formation, underscoring the delicate balance between genetic predisposition and environmental factors in shaping fetal development. Teratogens are those substances which may cause inheritable birth defect via a toxic effect on an embryo or foetus. These defects include malformation, disruption, deformation and dysplasia which may cause stunted growth, congenital disorders that lack structural malformation. A fundamental principle of teratology is that the susceptibility to teratogenic agents those causing malformations vary with the embryo's developmental stage at the time of exposure. Exposure during the first three weeks of gestation (stage of preformation), when the basic body plan is established, typically results in early embryonic death or is compensated for by regulatory mechanisms within the embryo. Agents that disrupt the morula or blastocyst stages, or interfere with embryo maternal communication and proper implantation in the uterine mucosa, often lead to early embryonic mortality. The severity of malformation is depends on the frequency and dose of the teratogens. Most of the teratogens affect the embryo during early differentiation and organogenesis stage. It has been found that the organogenesis of urogenital system of bovines, equine, canines, porcine and felines are most susceptible for teratogenic agents.

Introduction

Lymphatic tissue in general consists of reticulin fibres and fixed cells (macrophages and reticulin cells), as well as free cells (lymphocytes, plasma cells and granulocytes). In loose lymphatic tissue, fixed cells predominate whereas, in dense lymphatic tissue, free cells predominate. The stem cells which colonize the embryonic thymus and bursa originate in the area pellucida or embryo proper in birds. Late in embryonic development, stem cells may be found in the liver and spleen. In adult life they exist primarily in the bone marrow. The avian lymphatic system is divided into:

A. Primary lymphatic tissue (central): Primary lymphoid organs in birds include the thymus and the bursa of Fabricius, which are the places where lymphocytes develop, and the T-cell and B-cell receptor genes rearrange. The avian primary lymphoid organs, the thymus and the bursa of Fabricius, undergo age-dependent changes leading in some cases to the complete atrophy of the organ. Interestingly, the bursa of Fabricius can act as both i.e. primary and secondary lymphoid organ. Primary lymphatic tissue is antigen dependant, and in it incoming stem cells differentiate into immunologically competent B and T cells, which then migrate to the secondary lymphatic tissue

B. Secondary lymphatic tissue (peripheral): It consists of the spleen, gut-associated lymphoid tissue (GALT), including the caecal tonsil, lymph nodes, bone marrow, mural lymphoid nodules and variable aggregations of lymphocytes throughout the body. Secondary lymphatic tissue is antigen dependent "effector" tissue, in which B and T cells mature into respective effector cells.

Introduction Adaptations in animals and birds are countless and extensive, which permit the species better suited in its environment and thus improve chances of survival and propagative success. There are three major categories of adaptations namely anatomical/structural, physiological and behavioural adaptations. Depending on the animal habitats, adaptations can be classified into terrestrial, aquatic and volant (flying). Terrestrial adaptations are exhibited by the organisms inhabiting in land habitats and are again grouped into cursorial, fossorial, arboreal and desert adaptations.

Terrestrial Adaptations

1. Cursorial Adaptations

Cursorial animals are those adapted for running over long distances, often characterized by adaptations that enhance speed and endurance. These adaptations include various skeletal features that optimize their locomotion and overall efficiency while running. These animals have streamlined and spindle-shaped body to minimize resistance in attaining the speed. Here are the key skeletal adaptations in cursorial mammals:

1. Long and Slender Limbs: Cursorial mammals typically have long and slender limbs, which reduce the amount of energy required per stride and increase stride length, thereby enhancing running speed. Lengthening of the limbs in these animals leads to the head placed away from the ground. Hence, as an adaptation to feeding and drinking, the neck and the skull become elongated.

2. Reduced Number of Toes: Many cursorial mammals have reduced the number of toes to streamline the foot and reduce weight. For example, horses typically have a single functional toe (hoof), while cheetahs have reduced and non-retractable claws.

Introduction

A deep knowledge of veterinary anatomy is essential for more sustainable livestock management practices, enhancing productivity while minimizing environmental impact. Understanding the physiological structure and function of food-producing animals enables professionals to make informed decisions regarding production, meat processing, and food safety. In today's modern workforce, a comprehensive grasp of animal anatomy is essential for success in various fields, including agriculture, veterinary science, food processing, and quality control. Professionals can enhance their expertise, make data-driven decisions, and drive innovation in the food production sector by grasping animal anatomy. Ultimately, this foundational knowledge is vital for advancing careers and ensuring the highest standards of quality, efficiency, and safety in the industry. Meat processing professionals require in-depth knowledge of animal anatomy to guarantee optimal carcass handling, accurate meat quality evaluation, and streamlined production workflows. Expertise in animal anatomy is also crucial for food safety and quality control specialists, enabling them to ensure regulatory compliance and maintain consumer trust. Additionally, knowledge of animal anatomy allows these specialists to identify potential contaminants and implement effective control measures. By possessing a thorough understanding of animal anatomy, industry professionals can optimize efficiency, quality, and safety throughout the meat processing and distribution chain.

Introduction

Veterinary Anatomy is regarded as the firm foundation and the knowledge gained in the subject is the basis for reasoning and helping with the diagnosis and treatment of animals. Teaching gross anatomy is highly regarded as being clinically relevant. The research on a wide range of species in Veterinary anatomy will be helping to be the understanding of their biology. The research conducted helps to understand the anatomy of domestic and wild animals and how this can be applied to diagnostic imaging and other disciplines of Veterinary Science. Hence teaching and research in anatomy provides foundation on which the knowledge of clinical practice and medicine is built. The research in Anatomy pave way for advanced research in other Veterinary Discipline.

TEACHING PERSPECTIVES

Anatomy education has entered newer and more innovative methods of teaching. “Vesalius” referred to as a founder of modern Anatomy struggled to make dissection of cadavers mandatory in 16th century and its significance is again being questioned today in search of substitutes. Hence use of alternatives to dissected specimens and multimedia teaching methodologies to teach anatomy and basic principles of Veterinary Medicine and Veterinary Surgery have gradually become more popular.

Bovine leukocyte adhesion deficiency (BLAD) is a lethal autosomal recessive disease caused by a point mutation in CD 18 gene. Through Minimum Standard Protocol (MSP), Government of India made mandatory to screen all the breeding and newly introduced HF bulls for this BLAD. In this present study screening for mutation is carried out by polymerase chain reaction-restriction fragment length polymorphism technique (PCR-RFLP). Non availability of positive control made us to resort for synthesis of DNA probes with similar sequence of the gene with mutation and authenticity was verified by nucleotide sequencing. A total of 200 blood samples collected from phenotypically normal HF bulls from organized farms and subjected to PCR-RFLP technique. The results indicate that out of 200 bulls, 01 bull (0.5%) was found to be carrier for mutated gene CD18. Hence it is mandatory to screen each bull to prevent accidental transmission of mutant gene to a large number of future populations.

Formalin, traditionally used for preserving anatomical specimens in veterinary education, poses well-documented hazards like toxicity and carcinogenicity. This proposal advocates for adopting safer, innovative preservation methods that maintain anatomical integrity and meet modern educational and ethical standards. The study aims to evaluate various formalin-free preservation methods: plastination, cryopreservation, resin embedding, and 3D scanning and printing. Plastination involves fixing specimens in 10% formalin, dehydrating in acetone, impregnating in glycerine, and curing in corn starch. Cryopreservation rapidly freezes tissues in liquid nitrogen, all chemical reactions, and biological processes are suspended. Resin embedding encapsulates specimens in epoxy resin, and advanced imaging creates detailed, interactive 3D models. Plastination maintains anatomical integrity and durability better than traditional formalin, retaining original structures with minimal distortion, and providing long-lasting educational resources. Cryopreservation preserves tissue well but requires specific storage conditions. Resin embedding produces clear, durable specimens but is less flexible for handling. 3D printing offers highly detailed, interactive models without chemical exposure risks. All methods avoid formalin’s toxic properties enhancing safety for students and educators. Transitioning to formalin-free preservation methods improves health and safety by eliminating toxic exposure and enhances educational value with more interactive and detailed models. These methods promote sustainability and align with modern ethical standards. Advanced imaging supports contemporary education by providing precise, manipulatable anatomical representations in a virtual environment. Adopting innovative preservation methods such as plastination, cryopreservation, resin embedding, and advanced imaging technologies ensures safer, high-quality anatomical education aligned with modern standards of Veterinary anatomy education.

The current study focused to investigate the significance of AgNOR count in diagnosis of canine mammary tumour and its correlation with histopathological evaluation. A total of 53 cases suspected for canine mammary tumour were presented to Veterinary Clinical Complex, DUVASU Mathura from January 2021 to April 2022, out of these 42 cases were screened as neoplastic while 11 were non- neoplastic based on cytological examination. 24 tissue samples were collected out of 42 neoplastic cases during surgical procedure under general anaesthesia for further detailed examination by the approval of Institutional Animal Ethics Committee vide application number IAEC/22/22. AgNOR staining revealed that carcinoma solid had highest number of AgNOR followed by malignant myoepithelioma and fibrosarcoma. AgNOR count gives an assessment of cell kinetics and is directly related to proliferative activity of cells.

Globally the field of veterinary is dominated by women as 55% of veterinarians are women. In India 76% girl graduates were in the year 2021. Lady Veterinarians have been successful at their jobs especially in recent years. This brings out whether there is a psychological relation to this. “Animals are good companions for women.”, said Sreeja Arangottukara to The Hindu.[1] Gender is an identity which develops in a child around the age of 2 or 3 which is influenced by socialisation, culture, and biology.(Penn State College of Liberal Studies)[2] Various psychological researches have shown that women and men differ in intensity of certain attributes. From the ages of 21-64 years assessed with Rytt’s Psychological Well Being Scales and Bem Sex Role Inventory conducted under general Spanish population of 1700 men and 1700 women.(National Library of Medicine) It was found that women were better in positive relations which helps in developing trust between the veterinary team and clients and achieving a positive public perception of the veterinary profession.(today’s veterinary practices)[3] Women are better at understanding facial and body cues which lead them to understand the requirement of the client/patient in medicinal branch of veterinary. Moreover, women are better at verbal skills too their memory and analysing ability makes them capable of research and advancement in the field of animal husbandry. In a profession like veterinary women have better psychological ability and resultingly better at their job.

Antimicrobial resistance (AMR) includes antimicrobial use and abuse in human, animal and environmental sectors. It also covers the spread of resistant bacteria and resistance determinants within and between these sectors and as global phenomenon. Most of the classes of antimicrobials used to treat bacterial infections in humans are also used in animals. Major concerns in the animal health are treatment of animals with antimicrobials that are critically important for humans too, such as third-generation cephalosporins and fluoroquinolones. Another important issue is the use of medically important antimicrobials in feed for long term, such as colistin, tetracyclines, and macrolides, for growth promotion. Inappropriate use and unprofessional choice of antibiotics without performing antibiotic susceptibility test in animals results into higher occurrence of antimicrobial resistance. Given the important and interdependent human, animal and environmental dimensions of AMR, it is logical to take a One Health approach when addressing this problem. One Health is the multidisciplinary, collaborative, scientific and coordinated approach to address health threat at human, animal and environment interface, the aim of One Health approach is to understanding and designing interventions and policies to combat AMR. WHO recently has launched new guidelines on the use of medically important antimicrobials in food-producing animals, recommending that farmers and the food industry stop using antimicrobials routinely to promote growth and prevent disease in healthy animals. Thus these guidelines will help to preserve the effectiveness of antimicrobials that are important for human medicine by reducing their use in animals.

A study was conducted to assess utilization pattern of ICT based Information by the livestock owners in using information communication technology with special reference to enhanced livestock production, in Mathura district of Uttar Pradesh. A total of 80 farmers were selected for study. The results showed that cent percent of the farmers had availability and accessibility of mobile phones as well as television. Majority of the livestock farmers are regularly using ICT tools for information dissemination. Majority (85.00%) of respondents were saying scientific information of livestock is available on mobile followed by (25.00%) of respondents said this scientific information are available on computer. Apart from these (68.75%) of respondent usage of mobile as ICTs tools for getting health related information of animals. Mobile phone was the most frequently and widely used while radio was least used ICT tool among the livestock farmers being used for the purpose of getting information.

Ruminant, major source of meat and milk for human consumption, whose digestive system has evolved in such a way that it provides an additional advantage of being non-competitive to human food chain. Global warming is real and rising with a pace threatening lives of all flora and fauna on earth. Methane produced by ruminant bacteria predominately by methanogen group of bacteria acting as hydrogen sink cause loss of dietary energy to the animal affecting its potential productivity as well as a major source of global warming. The use of plant secondary metabolites is preferred as a feed additive in ruminant diet to improve fatty acid production by reducing enteric methane. The study was carried out using 10% aqueous and ethanolic extract of Psidiumguajawa leaves powder, citrus fruit peel powder to assess in vitro rumen fermentation parameters. The in vitro trial was conducted by taking different concentrations @ 0.2 ml, 0.5ml, 1ml and 2 ml of plant extracts with substrate media containing rumen fluid. After 6 and 24 hours of incubation, pH, total gas production, ammonia- nitrogen, TVFA and total nitrogen were measured. Bacteroides and methanogenic bacterial profile was done by Real time PCR. The results revealed that the total gas production decreased in plant extract supplemented samples with respect to control. Total nitrogen production increased in aqueous P. guajawa and increase in TVFA was observed in all plant extract supplemented samples. Ammonia- nitrogen production does not vary significantly, indicating no change in NPN degradation due to supplementation of different plant extracts at different concentration. Total bacteroides expression were increased in rumen samples treated with P. guajawa and citrus peel powder, while a decrease in methanogenic bacterial expression was observed in plant extracts supplemented samples as compared to control indicating an improvement in rumen fermentation further enhancing productivity.

The present study was conducted on 36 adult dogs of six different breeds in order to standardize normal ocular parameters. Six breed specific groups of dogs were Group 1 (German Shepherd), Group 2 (Pug), Group 3 (Spitz), Group 4 (Labrador Retriever), Group 5 (Rottweiler) and Group 6 (Non-descript) with 6 animals (3 males and 3 females) each. STT values revealed non-significant difference between sex and eyes. Among groups only Pug breed showed significant difference with GSD breed. Intraocular pressure reflected a non-significant difference between sex and eyes. IOP values of Labrador group were within the normal range but differ significantly from Pug and Non-descript groups. B mode ultrasonography revealed internal structures with various echogenicity. In B mode ultrasonographic biometry a significant difference in all parameters was observed among groups except ODL and OND. Longest and shortest axial globe length was revealed in Labrador and Spitz, respectively. Overall mean for sex revealed a longer vitreous chamber depth, axial globe length and optic disc length in males as compared to females. Non-significant difference was observed in all the parameters between left and right eyes. Standardized breed specific ophthalmic parameters were useful in diagnosing various ophthalmic affections, viz. keratoconjunctivitissicca, glaucoma, cataract, retinal detachment etc. in clinical cases.

Introduction

The compassionate treatment of animals and consideration for their well-being is an important aspect of livestock farming. Compassions needs to be an integral part of livestock farming as livestock are sentient being. Positive human experiences during handling are essential for ensuring the welfare of animals. Humans play a significant role in the environment of production animals, particularly in commercial cattle production where daily interaction occurs. Intensively farmed animals endure negative emotions that diminish their welfare. For instance, they experience fear from unfamiliar stimuli or from being separated from their social group (Jones, 2011), as well as stress, pain and frustration due to the obstruction of naturally driven behaviors (Dawkins 1998) or the absence of predictability and control over their surroundings (Bassett et al, 2007), along with boredom stemming from insufficient environmental stimulation (Wemelsfelder, 1993). Compassion is a fundamental virtue in veterinary practice, and female-owned businesses in this sector often exhibit this attitude. Compassion, the highest quality of women, is highly valued in veterinary practice, and women-owned enterprises are perfect example of this. They place a high value on compassionate care, acknowledging the close bond between people and animals and making sure each animal gets the attention and assistance they require by focusing on improving the quality of life for all animals and their loving owners.

A novel zoonotic disease was reported in December 2019 in Wuhan (China) and patients were presumably exposed to a new coronavirus, designated as SARS CoV-2. This virus probably crossed into humans from an animal species, most likely a bat, spreading rapidly across the world and causing the coronavirus disease 2019 (COVID-19). This infectious illness was responsible for a global pandemic that caused deaths and economic despair (Santaniello et al., 2023). The latest WHO data (December 2023) confirmed 77,38,19,856 cases worldwide since the beginning of this zoonosis and 70,10,568 deaths (WHO, 2023), making this pandemic one of the deadliest in human history. Human CoVs (HCoVs) are often of animal origin but adapt to humans via direct jumping or through intermediate species. These data and the zoonotic origin of SARS-CoV-2 quickly raised questions concerning the potential role that animals could play in transmitting the virus to humans or other species.

Introduction

In recent years, women have made remarkable strides in the veterinary field, bringing a wealth of skill and dedication to various sectors, including clinical practice, research, academia, and industry. Today, women comprise a significant portion of the veterinary workforce. However, the path to leadership and career advancement still presents unique challenges. Gender biases, limited representation in senior roles, and the constant balancing act between personal and professional life often stand in the way. As a woman in this field, I understand the significance of leadership development and the impact it has on creating a meaningful, rewarding career. This session aims to equip women veterinarians with actionable strategies and essential leadership skills to advance their careers meaningfully. Leadership is not merely a title; it’s about creating positive change, setting examples, and inspiring those around us. For women in veterinary medicine, it means breaking barriers, making a difference, and contributing to animal welfare and public health. By cultivating confidence and a focused approach, women can rise to leadership roles that truly make an impact.

Introduction

Milk, which is recognized for its significant nutritional and immunological value to humans as well as animals, is the primary source of vital nutrients and bioactive components that contribute to the newborn’s growth and development. In addition, it includes extracellular vesicles (EVs). EVs are membrane-bound vesicles that are discharged by cells into biofluids like milk. EVs play an important function in intercellular communication and have demonstrated potential in therapeutic applications. This is extremely important since EVs are abundant in milk and carry a wide range of cargo, including proteins, lipids, and nucleic acids, that supports to their classification as bioactive components of milk Milk-derived exosomes have intriguing properties as nanocarriers for theranostic care.

Introduction

Biofilm formation is a series of complex process and accomplished mainly in five stages -Reversibleattachment, Irreversible attachment or colonization,Proliferation, Maturation &Dispersion. Usually biofilms comprise 10% of dry mass that represent microorganisms while the rest 90%derived from the matrix of biofilm. Microbial biofilms are aggregates of microorganisms embedded in self-produced matrices and adhere to an abiotic or biotic surface. Firstly, Marshall et al. (1971) threw light on the concept of biofilms &it’s complexity was discussed by many researchers. According to Donlan and Costerton (2002) “Biofilm as a structured community of microbial cells enclosed in a self-produced polymeric matrix and adherent to a surface to interface, and to each other” still remains the most appreciated definition of biofilms (Mishra et al., 2022). Basically, microbial biofilms are complex, dynamic and three dimensional heterogeneous structures in which cells are interconnected by EPS (Extracellular Polymeric Substances). Biofilms are found to be resistant against UV light, metal toxicity, antimicrobials and other chemical agents like soaps, detergents, disinfectant and other cleaning agents. Future insight the immense need to implicate effective methods for elimination of biofilms from the environment.

Introduction

Diagnosis of cancer has always been challenging. There is no single diagnostic test to confirm the cancer. A detailed evaluation of the patient comprises of history, physical examination and various diagnostic tests.

Most common steps in the diagnosis involve physical examination, imaging tests, laboratory tests, biopsy etc. Other than these there are several other battery of tests that are followed in modern diagnostics. The main aim of the diagnosis is to determine what type of cancer and more importantly the stage and the grade. Early detection of cancer provides cure or better chances of survival in any patient. It improves the prognosis of cancer and potentiate the effect of targeted therapy. There are several moderndiagnostic techniques like 2D and 3D imaging using PET positron emission tomography, MRI, Computed Tomography (CT), X Rayimaging and ultrasound scanning. The diagnosis of tumor involves several steps.

Introduction

My journey into the heart of wildlife conservation began in the unassuming classrooms of KendriyaVidyalaya, where my curiosity for the natural world was nurtured by dedicated teachers and an enriching curriculum. My dreams took f light when I studied stories of Mahadevi Verma in my school days where she was taking care of wild animals in her home. Fuelled by my determination, I pursued my education in Veterinary College, Hebbal, Bangalore from 2006 to 2011. To increase my knowledge and help the wild animals in better way, I did master in Veterinary Microbiology from Veterinary College, Hebbal, Bangalore during 2012-2014, research was conducted in “Salmonellosis in Wild Animals” and PG diploma course in wild animal disease management from TANVAS, Chennai in 2016 research was on “Oral Microflora of Snakes”. Pursing higher studies gave direction to help wild animal, also gave me abilities to write research paper which will help the communities.

Introduction

Goat farming plays a significant role in supporting rural livelihoods, contributing to food security, and generating income through milk, meat, and fiber production. However, achieving sustainable goat productivity requires scientific management, wherein, nutrition is a critical factor that makes profound effect on overall performance and production. With increasing global demand for goat-derived products, optimizing nutritional strategies has become essential to improve both production efficiency and sustainability. Globally, the sector is shifting from subsistence-oriented backyard farming to more commercialized systems, driven by increasing demand for goat meat and dairy products. India, which ranks first in goat population with 148.88 million goats (DADF, 2019), is experiencing rapid changes as goat farming evolves from a low-input livelihood strategy into a thriving commercial industry (Ramachandran et al., 2019).This transformation is not only economic but also ecological. Goats are known for their remarkable adaptability to harsh environments and climate variability, which makes them crucial in ensuring food security under changing environmental conditions. Their ability to utilize marginal lands and convert low-quality forages into meat, milk, and fiber allows them to thrive in regions unsuitable for other livestock . Shifting towards semi-intensive and intensive systems, farmers can utilize better breeding, nutrition, and healthcare practices to meet growing market demands.

Introduction

In current scenario of COVID-19 pandemic, Agriculture and its allied sectorare the only sector which is contributed positive growth rate due to hard work and total devotion of farmers towards it in both first quarter of the financial year 2022-23. Farmers are the backbone of our country but face different challenges such as scarcity of land due to fragmentation, water sources, impassable roads, unavailability of better financial services and new technologies. Since a decade number of small and marginal farmers keeps on increasing. Due to the fragmented holding of land and improper management practices in farming inadequate access to market, making them agriculture more unviable. Government motive to double the farmers’ income in 2022-23 by addressing the challenges of farm sector. The government affirmed that farmer producer organizations (FPO) are the most appropriate institutional form around which farmers can mobilize and build their capacity to collectively leverage their production and marketing strength. However, majority of these located in rural areas and suffer from the problems like Inadequate Professional Management and Manpower, poor start-up capital, lack of technically skilled man power, ineffective capacity building training programmes. There is a need to find the solution and to need bring policy changes to handle this problem to empower our farmers and Indian economy by farmers produce organisation.