eChapters

Biological control is a strategy which employs utilization of natural enemies to reduce the damage caused by noxious organisms to tolerable levels (De Bach and Rosen, 1991). This is the most benign method for reducing pest population in vegetable ecosystem. It opens an amicable platform for production of organic vegetables with good export value. Since the late 1950s, much of the insect control in vegetables has been based on the use of synthetic chemical insecticides.

Introduction

The tropics extends on  vast region of the earth surface ranging from 23½^°C North to the Tropic of Cancer and 23½^°C South to the Tropic of Capricorn. Several countries of Asia, Africa and Latin America include tropical areas. The tropical regions are the warmest area on the earth with average temperatures ranging between 20^°C and 35^°C. More than 80 % of the area in tropics supports forests and savannah and only 15% is under deserts, shrubs and grasses. About 5% of the area is under mid or high altitude with moderate climatic conditions. Evidently, the tropics support great diversity of physical and climatic conditions in different ecologies and different ecosystems. Temperature and daylight length is much higher throughout the year which induces more number of insect pests, their generations causing more damage in tropics. The increased menace of insect pests requires frequent and repeated use of chemical pesticide to mitigate the insect pest damage.

1.0 INTRODUCTION

“Biological control is the reduction of the amount of inoculum or disease producing activity of a pathogen  or parasite  in its active or dormant state, by one or more organisms, accomplished naturally or through manipulation of the environment, host or antagonist or by mass introduction of one or more antagonists ” (Baker & Cook 1974). The biological world is a vast interacting network of living organisms in a state of dynamic equilibrium, reflecting changes in their physical environment and their relations to each other. Organisms exhibit many type of interactions in the process of achieving biological balance.  An organism may exert no effect on another or it may stimulate or inhibit the growth or development of the associate, it may stimulate or inhibit the formation of resting spores of the associate or it may enforce dormancy or cause lysis of the associate (Cooke & Baker, 1983).

1.0 INTRODUCTION

Infestation by insect pests is a major factor responsible for the low productivity of spice crops in India. Spice crops such as black pepper (Piper nigrum L.), cardamom (Elettaria cardamomum Maton), ginger (Zingiber officinale Roscoe) and turmeric (Curcuma longa L.) are infested by many species of insect pests, some of which cause severe crop losses. Adoption of integrated pest management (IPM) schedules in which biological control forms a major component is more relevant in these crops since excessive use of insecticides could lead to pesticide residues in the produce and other ecological hazards. Spices are high-value and export-oriented in nature and pesticide residues are the most important non-tariff barriers on trade in these produces. There has been a renewed interest during the past decade in developing environment–friendly crop management schedules in sustainable agriculture including spice crops, reflecting the increasing concern over pesticide misuse.

Introduction

The term “biological control” was first used by Smith in 1919 to signify the use of natural enemies (whether introduced or otherwise manipulated) to control insect pests. DeBach (1964) defined biological control as the action of parasites, predators, or pathogens that maintain another organism's population density at a lower average than would occur in their absence (Dhaliwal and Arora, 2016). According to a recent definition by Eilenberg et al. (2001) biological control or biocontrol is “The use of living organisms to suppress the population density or impact of a specific pest organism, making it less abundant or less damaging than it would otherwise be.

INTRODUCTION

Plant diseases need to be managed for maintaining the quality and abundance of food, feed, and fiber produced by growers around the world. Different approaches may be used to prevent, mitigate or control plant diseases. Beyond good agronomic and horticultural practices, growers often rely heavily on chemical fertilizers and pesticides. Such inputs to agriculture have contributed significantly to the spectacular improvements in crop productivity and quality over the past 100 years. However, the environmental pollution caused by excessive use and misuse of agrochemicals, as well as fear-mongering by some opponents of pesticides, has led to considerable changes in people’s attitudes towards the use of pesticides in agriculture. Today, there are strict regulations on chemical pesticidal use, and there is political pressure to remove the most hazardous chemicals from the market.

Introduction

Soil supports richest number and kinds of microorganisms interacting with each other and thus by modification or alteration in soil conditions greatly influence the microbial community and their activity in soil ecosystem. Soil physico-chemical and the biological factors interact to provide rapidly changing ecological niches and microbial components fluctuate in response to change in soil conditions. There are large evidences that agricultural practices often favour antagonistic microorganisms (Cook and Baker,1983; Yarham,1979) and therefore, biological control of plant pathogens could be possible through manipulation of soil condition. Soil organic matter also has a profound influence on microorganisms in soil, particularly those, including some pathogens, saprophytic and obligate plant parasites. Soil organic matter is distributed at discrete sites within the soil and determines to a great extent which parts of pore space are occupied. 

INTRODUCTION

Fungi naturally present in soil and rhizosphere of various crops serve as potential biological control agents of various plant pathogens. Species of fungi occurring in the genus Trichoderma have gained significant importance for use as agents for control of several diseases of cultivated crops. The last decade has witnessed a tremendous break-through in the research efforts on biological control of plant diseases in India especially by using species of Trichoderma and Gliocladium. Several economically important plant diseases of a variety of crops were effectively managed by using Trichoderma based biofungicides.

The work on biocontrol of pigeonpea wilt by Bacillus subtilis by Dr. R S Vasudeva and his associates, published as a series of papers in Annals of Applied Biology during 1950 to 1965 can be considered as a starting point for Indian work on biological control of plant pathogens (Mukhopadhyay, 1994).

Bioagents like insects, pathogens etc., and other animals are used either to reduce growth or kill weeds.

But, not possible to eradicate weeds.

Introduction

Globally, India takes pride of being one of the world’s largest producers of horticultural and agricultural commodities. The rapid increase in the area under intensive cultivation of crops, introduction of high yielding varieties, lack of natural enemy load of the pests and changing climatic conditions is largely responsible for the severe pest depredations. Pest management in agriculture is a challenging task in the context of increasing agricultural productivity without upsetting the ecological balance and deteriorating the environment. Use of agrochemicals in agriculture though played a significant role to boost the agricultural production is posing enormous problems like environmental pollution, pest resistance, pest resurgence, toxicity hazards, secondary pest outbreaks, residues in feeds, foods, soil and water, destruction of biodiversity of useful natural enemies and some social economic and political problems. In response, there has been increased demand for alternative and selective pest control agents. Biopesticides are advantageous due to their target specificity, eco-safety, no development of resistance, reduced number of applications, yield and quality improvement, higher acceptability and value of produce for exports and suitability for rural areas.

Deliberate use of natural enemies to control invasive pest in an ecosystem is known as biocontrol. To control invasive weed species, selective pathogens and weed seed predators are employed to reduce the population of the weed to non competitive numbers rather than total eradication of the weed species (Appleby, 2005) These can be insects, herbivorous fish, other animals, disease causing organisms or even competitive plants. This method is comparatively safer to the ecosystem, is cheaper and has long lasting impact. Difficulty in f inding host specific bio-agent with minimum adverse effect on other crop and the environment is a challenge.

Abstract

Since the first oil well drilled successfully in 1859, crude oil has been a serious problem around world, including Indonesia. As a source of all petroleum products, crude oil is very complex component and divided into aliphatic, aromatic, resin and asphaltene. Petroleum products have been used for daily life and created large amounts of pollutants in environment and ecosystems around the globe. The pollutants should be treated by certain method; among them, bioremediation has several advantages to resolve the problem. In 2003, Ministry of Environmental (MoE) – Republic Indonesia was endorsed with a new regulation about biological treatment or bioremediation for crude oil contaminated soil (MoE regulation No. 128/2003). The most important to be noted in bioremediation process is the presence of microbes with certain metabolite capabilities. The degradation rate also can be improved and sustained by ensuring the adequate required factors. This article describes the removal of crude oil by utilization of microbial potentials, factors affecting biodegradation, and how they are applied in Indonesia.

The increasing concerns about dwindling biological resources globally led to the Convention on Biological Diversity in 1992. This convention for the first time, recognized sovereign rights of States over their biological resources and emphasized that access to genetic resources should be only for environmentally sound purposes and should be subject to national legislations. The access has to be on mutually agreed terms which inter-alia would include recognition of associated Traditional Knowledge of indigenous communities and equitable benefit sharing arrangements.

Taking cognizance of the provisions of the CBD, and to deal with extensive pressure on our biological resources, India has enacted an umbrella legislation called Biological Diversity Act-2002 (No. 18 of 2003), and also notified the Biological Diversity Rules-2004 for guidance and compliance by various stakeholders, including the Union and State Governments, Non-state sectors, and individuals. The Act aims at conservation of biological resources as well as facilitating access to them in a sustainable manner and through a just process. The salient features of this act are as follows (NBA, 2004).

The increasing concerns about dwindling biological resources globally led to the Convention on Biological Diversity in 1992. This convention for the first time, recognized sovereign rights of States over their biological resources and emphasized that access to genetic resources should be only for environmentally sound purposes and should be subject to national legislations. The access has to be on mutually agreed terms which inter-alia would include recognition of associated Traditional Knowledge of indigenous communities and equitable benefit sharing arrangements.

Plant nutrients are drawn up from soil for growth and development. There are more than 90 elements present in the soil. According to the criteria of Essentiality of Nutrients for plant growth, proposed by Arnon and Stout in 1952, only 16 are considered to be essential for plant growth. Out of these, C (Carbon), H (Hydrogen), O (Oxgen), N (Nitrogen), P (Phosphorus), K (Potassium), Ca (Calcium), Mg (Magnesium) and S (Sulfur) are called Macronutrients (required in large quantities) and the remaining 7 elements - Fe (Iron), Mn (Manganese), Cu (Copper), Zn (Zinc), B (Boran), Mo and Cl are called Micronutrients or trace elements (required only in very small quantities). Among the macro elements - N, P and K are called Fertiliser elements or primary nutrients as they are most often applied in the form of synthetic or mineral fertilisers, and Ca, Mg and S are called secondary elements which are applied in smaller quantities than primary nutrients and required subsequent to primary nutrients.

Plant diseases need to be controlled to maintain the quality and abundance of food, feed, and fiber produced by growers around the world. Different approaches may be used to prevent, mitigate or control plant diseases. Beyond good agronomic and horticultural practices, growers often rely heavily on chemical fertilizers and pesticides. Such inputs to agriculture have contributed significantly to the spectacular improvements in crop productivity and quality over the past 100 years. However, the environmental pollution caused by excessive use and misuse of agrochemicals, as well as fear-mongering by some opponents of pesticides, has led to considerable changes in people’s attitudes towards the use of pesticides in agriculture. Today, there are strict regulations on chemical pesticide use, and there is political pressure to remove the most hazardous chemicals from the market. Additionally, the spread of plant diseases in natural ecosystems may preclude successful application of chemicals, because of the scale to which such applications might have to be applied. Consequently, some pest management researchers have focused their efforts on developing alternative inputs to synthetic chemicals for controlling pests and diseases. Among these alternatives are those referred to as biological controls.

Definition

Garret (1970) defined biological control as any condition under which or practice whereby survival or activity of a pathogen is reduced through the agency of any other living organism (except man) with the result that there is a reduction in the incidence of the disease caused by that pathogen.

Baker and Cook (1974) stated that the biological control is the reduction of inoculum density or disease producing activities of a pathogen or parasite in its active or dormant stage, by one or more organisms, accomplished naturally or through manipulation of the environment, host or antagonists, or by mass introduction of one or more antagonists.

Biocontrol can be simply defined as the application of living organism to control diseases.

Managing plant diseases is necessary to preserve the quantity and quality of food, feed, and fiber that producers worldwide produce. To avoid, lessen, or manage plant diseases, several strategies may be employed. Aside from using effective horticultural and agronomic techniques, growers frequently largely depend on chemical pesticides and fertilizers. The remarkable increases in crop productivity and quality over the past 100 years have been largely attributed to such agricultural inputs. However, fear-mongering by certain opponents of pesticides and environmental degradation from excessive and improper use of agrochemicals have prompted significant shifts in public perceptions toward pesticide usage in agriculture. Political pressure is present to remove the most dangerous chemicals from the market, and there are stringent limits on the use of chemical pesticides today. Furthermore, because of the potential scale at which such applications could need to be made, the proliferation of plant diseases in natural ecosystems may make the successful application of pesticides impossible. As a result, several researchers studying pest management have concentrated on creating substitutes for artificial chemicals in the management of diseases and pests. Biological controls are one type of alternative among them.

COMMON FEATURES OF BIOLOGICAL MEMBRANES

Membranes are sheetlike, just a few molecules thick and form closed boundaries between cell compartments.

Membranes contain lipids and proteins, with small amounts of carbohydrayes linked to the lipids and proteins.

Lipids in membranes are small with hydrophobic and hydrophilic portions. Lipid bilayers provide a barrier to the diffusion of polar molecules.

Characteristic functions of membranes are mediated by specific proteins, serving as pumps, channels, receptors, energy transducers and enzymes.

Membrane components associate through noncovalent interactions.

Membranes are asymmetrical, with two sides of the membrane differing from each other.

Lipid and protein molecules often diffuse rapidly in the plane of the membrane.

Central transport of ions and molecules into and out of the cell.

Generate proton gradients for ATP production by oxidative phosphorylation.

Receptors bind extracellular signals and transduce the signal to cell interior.

Biological control in plant pathology refers to the use of living organisms to suppress or control plant pathogens, pests, and diseases, thereby reducing the need for chemical pesticides. These living organisms, known as biological control agents (BCAs), can be naturally occurring or introduced, and they act by various mechanisms to inhibit or eliminate the pathogen. These methods harness natural enemies of pathogens such as antagonistic fungi, bacteria, or other beneficial organisms to reduce the population of harmful pathogens in the environment. Biological control is an important component of sustainable agriculture, offering an eco-friendly alternative to chemical pesticides and contributing to integrated disease management (IDM).
Key Mechanisms of Biological Control
1. Predation: Certain organisms, like Trichoderma species or predatory mites, directly attack and consume plant pathogens, reducing their population.
2. Parasitism: Some fungi, like Ampelomyces quisqualis, parasitize plant pathogens, such as powdery mildew, thereby suppressing their growth and reproduction.
3. Antibiosis: Microorganisms like Bacillus subtilis and Pseudomonas fluorescens produce antibiotics that inhibit the growth or kill plant pathogens. For example, Bacillus subtilis produces iturin, which suppresses fungal growth.

Nanoparticles synthesis is done by two major routes, which include classical synthesis and green synthesis. The green synthesis techniques generally utilize relatively non-toxic chemicals non-toxic solvents, biological extracts and systems. Biological methods are considered safe and ecologically sound forthe nanomaterial fabrication asan alternative to conventional physical and chemical methods

Nanoparticles

Gold, silver, and copper have been used mostly for the synthesis of stable dispersions of nanoparticles, which are useful in areas of photography, catalysis, biological labeling, photonics, optoelectronics and surface-enhanced Raman scattering (SERS) detection

The need for biosynthesis of nanoparticles rose as the physical and chemical processes were costly. So in the search of cheaper pathways for nanoparticle synthesis, scientists used microorganisms and then plant extracts for synthesis. Nature has devised various processes for the synthesis of nano- and micro- length scaled inorganic materials which have contributed tothedevelopment of relatively newandlargely unexplored area of research based on the biosynthesis of nanomaterials.

17.1 Introduction

Bioenergy is an array of energy produced by biomass (organic matter) including liquid, solid, and gaseous fuels, electricity, heat, steam, biochemicals and materials. Biofuel is any plant derived organic matter available on a renewable basis, including dedicated energy crops and trees, agricultural food and feed crops, agricultural crop wastes and residues, wood wastes and residues, aquatic plants, animal wastes, municipal wastes, and other waste materials. An organic matter such as wood, plants, residues from agriculture or forestry, and the organic component of municipal and industrial wastes that can now be used as an energy source. Today, many bioenergy resources are replenished through the cultivation of energy crops, such as fast-growing trees and grasses, called bioenergy feedstocks.

6.1 Introduction

Nanotechnology is the study of manipulating matter on an atomic or a molecular scale that deals with particle sizes between 1 and 100 nano meters at least in one dimension. Particles reduced to the nanoscale show some unusual properties which are different from what they exhibit on a macro scale, enabling unique systematic applications Tarafdar et al. (2012). The unexpected properties of nanoparticles are broadly due to the large surface area of the material, which dominates the contributions made by the very small quantities of the material. Nanoparticles, thus, take advantage of their dramatically increased surface area to volume ratio.

Nanotechnology has the potential to revolutionize the agricultural and food industry with new tools for the molecular treatment of diseases, rapid disease detection, nanofertilizer, enhancing the ability of plants to absorb nutrients etc. Nanotechnology opened doors to new ways of identifying and quantifying biomolecules through use of nanosensors and nanoprobes.

Introduction

After water, nitrogen is the most critical nutritive element for crop productivity and human efforts to produce food and energy have greatly changed the nitrogen cycle of the earth. Sustainable cropping systems throughout history and across the world have relied on the combination of a cereal with an N-fixing legume. In recent history, cereals have dominated global agriculture, while legume areas and productivity have stagnated or even declined. An atmosphere around us contains nearly 78% nitrogen, which is in free form. Hence it is not available to plants. At present, India produces around 206 million metric tones of foodgrains for its growing population, increased foodgrain cannot be produced unless we carefully make use of biological nitrogen. Biological nitrogen fixation is the key to sustain agricultural productivity through the application of biofertilizers in the field. However, there is an urgent need to transfer this technology on the field to farmers and industry by producing these fertilizers on large scale.

Abstract

Nitrogen (N₂) is an essential nutrient for every organisms. It is the building block of many biological molecules. Atmosphere contains 79% of N₂ gas which cannot be used by plants directly as it is chemically inert. Many diazotrophs like bacteria and cyanobacteria have the capability to convert atmospheric dinitrogen into a usable form by means of Biological nitrogen fixation (BNF). N₂ fixation is generally three types i.e. Asymbiotic, Associative and Symbiotic. Asymbiotic N₂-fixation also known as free living N₂-fixation is under taken by some bacteria and cyanobacteria. In associative N₂-fixation, some bacteria fix nitrogen in association with root of some grasses and cereal plants. In symbiotic nitrogen fixation, Rhizobium form root nodule in legumes and fix atmospheric N₂ and convert it into the ammonia (NH₃) by the help of Nitrogenase enzyme. This oxygen sensitive enzyme consist of two components i.e. di-nitrogenase (MoFe protein) and di-nitrogenase reductase (Fe protein) which are highly conserve in sequence and structure. The N₂ fixing genes e.g. nod gene (nodulation gene) and the nif gene (nitrogen-fixing gene) are located in the plasmid which is also called mega plasmid. Symbiotic association between legume and rhizobium have agronomic importantance and reduce the use of N-fertilizers or even avoided. The long term use of the nitrogenous biofertilizer in the agricultural field is a promising approach to develop and fulfil N₂ demand of the growing crop without causing any environmental hazard.

Abstract
Biological nitrogen fixation (BNF) is a critical ecological process carried out by grain legumes, which form symbiotic associations with nitrogen-fixing bacteria, primarily Rhizobium species. This natural mechanism converts atmospheric nitrogen (N2) into ammonia, a form that plants can readily utilize, significantly enhancing soil fertility and reducing the need for synthetic nitrogen fertilizers. The process begins with the recognition and infection of the legume roots by rhizobia, leading to the formation of specialized structures called nodules, where the bacteria convert atmospheric nitrogen into ammonia through the enzymatic action of nitrogenase. The symbiosis between legumes and rhizobia is tightly regulated by various genetic and environmental factors, including plant signalling molecules, soil conditions, and nutrient availability. Grain legumes, such as soybeans, chickpeas, and lentils, play a vital role in sustainable agriculture by improving soil health, promoting biodiversity, and providing a protein-rich food source. This overview explores the molecular mechanisms underlying BNF, the factors influencing the efficiency of this process, and its potential to enhance sustainable agricultural practices. Understanding the intricacies of BNF in grain legumes can inform breeding programs and management strategies aimed at maximizing the benefits of this natural process for agricultural productivity and environmental sustainability.
Keywords: Nitrogenase, Biological Nitrogen Fixation, Environmental sustainability, Soil fertility

Fixation of elemental nitrogen in the atmosphere by the microorganisms through a reductive process into ammonia is called biological nitrogen fixation (BNF). Molecular nitrogen (N₂) is the major component (approximately 80%) of the earth’s atmosphere. The element nitrogen is an essential part of many of the chemical compounds, such as proteins and nucleic acids, which are the basis of all life forms. However, N₂ cannot be used directly by biological systems to build the chemicals required for growth and reproduction. Before its incorporation into a living system, N₂ must first be combined with the element hydrogen. This process of reduction of N₂ commonly referred to as nitrogen fixation (N-fixation).

N₂is transformed into reactive N via lightning discharge (5 teragrams (Tg) N / year; 1 Tg equals 1012 g) and by biological N fixation (100 -140 Tg N /year ). Conversion of dinitrogen to ammonia in the industrial process requires temperatures of 400 - 500^o C and high pressures (Haber-Bosch process). Biological nitrogen fixation is much more important as it contributes to more than 70 % of the input in to the world’s soil and water nitrogen. It has been estimated that 100 to 175 million tones of nitrogen are fixed annually by the process of biological nitrogen fixation.

The process of N₂ fixation is mediated by the enzyme, called nitrogenase (which reduces N₂ to ammonia). The overall reaction is as follows.

The biochemical oxygen demand is a chemical procedure for determining the amount of dissolved oxygen needed by aerobic organisms in a water body to break the organic materials present in the given water sample at certain temperature over a specific period of time. BOD of water or polluted water is the amount of oxygen required for the biological decomposition of dissolved organic matter to occur under standard condition at a standardized time and temperature. Usually, the time is taken as 5 days and the temperature is 20°C. microbes
C6H12O6 + 6O2 ¾¾¾¾®CO2 + H2O
Principle
The test measures the molecular oxygen utilized during a specified incubation period for the biochemical degradation of organic material (carbonaceous demand) and the oxygen used to oxidize inorganic material such as sulfides and ferrous ion. It also may measure the amount of oxygen used to oxidize reduced forms of nitrogen (nitrogenous demand).
Procedure
Fill two BOD bottles with sample (or diluted sample); the bottles should be completely filled.

Phytoplankton populations are important in shrimp ponds because they aid in shrimp nutrition and the removal of excess nutrients from the pond environment. They can be beneficial or have potentially negative consequences, depending on how they are managed.

    Introduction
Over 60% of the population in India is occupied in agriculture and associated fields, making it one of the world’s most agriculturally dense nations. In India, the agricultural sector accounts for 18.6% of the country’s GDP. Almost all crops, including cereals, vegetables, and cash crops, are grown in India (Vanitha et al., 2013; APEDA, 2020). From the beginning of time, several pests, including weeds, insects, plant diseases, and nematodes, have had a detrimental effect on agriculture. As a result, crop losses are estimated to be 45%, or over 290 billion, annually (Aneja et al., 2016). Regardless of tactics, illnesses and pests significantly contribute to agricultural losses. Diseases, weeds, and insects hinder plant growth and lower plant density, eventually lowering the food supply. The Entomological Society of America (ESA) has released a policy statement stating that invasive insects cost more than $2.5 billion to control and cost $18 billion per year in economic losses to pastures, crops, lawns, and forests (The Not-So-Hidden Dangers of Invasive Species, 2018). Invasive crop pests continue to threaten food security because of the intricate relationships between human migration, international trade, climate change, and evolving agricultural techniques. Several of these variables also contribute to the sharp rise of urban pests and disease-carrying insects. The primary method of insect pest management has been chemical pesticides. Nonetheless, an increasing call for less agricultural chemical use due to worries about the environment, human health, and pesticide resistance ignites interest in novel ways.
Conventional chemical pesticides have raised food production and harmed non-target animals and the ecosystem. To prevent and mitigate pest-induced crop losses in storage (post-harvest losses) and in the field (pre-harvest losses). This has been accomplished through the development of numerous crop protection techniques. In the past few decades, chemical pesticides have been utilized to reduce food losses induced by insect pests; however, this has decreased agricultural production. Moreover, volatile pesticide residues have periodically raised questions about food safety among domestic customers and presented challenges to export crops’ ability to trade. Insecticides can cause physical or biological harm to pests. Certain insecticides are applied topically or indirectly to plants those insects may consume. Pesticides harm nature, soil, and human well-being, so regulations and rules governing their use are becoming more stringent. New technology and products to control and prevent pests are desperately needed. In modern agriculture, various methods are now available, including biological control, insect behavior, genetic modification, microbial pesticides and plant immunization of pest populations.

1. Introduction

Mounting pressure of teeming population and shrinking land resources have necessitated the production of increasingly more food per unit area. Insect pests, diseases and weeds are important biotic constraints inflicting 20-25% loss in agriculture production. Synthetic pesticides have of course, played a very significant role in restricting many pest problems. However, their indiscriminate has been its toll by creating several problems like pesticide resistance in insects, contamination of food with toxic residues, resurgence of pests and deleterious effects on non target organisms. The number of effective pesticides available is dwindling day by day further having less choice among which to choose. These ill effects have arised interest in alternate methods of plant protection.

The extent to which various plant species are utilized for pest control depends, not only on their effectiveness against target pests, but also on socio-economic, environmental and policy considerations. Keep these in mind the present study on the efficacy of botanicals against Spodoptera litura was carried out.

Abstract

Electronic waste or e-waste is one of the emerging problems in developed and developing countries worldwide. The constantly changing today’s world of technology has led to the serious problem of e-waste. The previous studies show that India has generated 0.6 million tons of e-waste in 2014 which may increase to 0.7 to 0.9 million tons by 2015–2016. E-waste constitutes multiple components some of which are toxic that can cause serious health and environmental issues if not handled properly. The challenge is to develop innovative and cost-effective solutions to decontaminate polluted environments, to make them safe for human habitation and consumption, and to protect the functioning of the ecosystems which support life. Biological approach is currently applied to recovery of metals from contaminated soil, groundwater, surface water, and sediments including air.

Tissue or cell samples from a living organism, different cell cultures grown in a laboratory incubator under controlled conditions, homogenates or extracts of cells and tissues, solutions of isolated and purified components (e.g. proteins, nucleic acids) can all be referred to as biological samples. As the medium of life is water, the majority of biological samples can be defined as aqueous solutions with one or more components, colloidal systems, or water-based suspensions (e.g. bacterial cells dispersed in a liquid medium). Consequently, most biochemical experiments also take place in aqueous environments. Different solids (e.g. chemical substances obtained from different companies, synthetic oligonucleotides or peptides) are also often necessary for biochemical research. In most cases, solids are dissolved in water (or sometimes in other solvents) prior to the experiments. Therefore, the methods of preparing solutions and measuring accurately the weight of the required solids will also be discussed below. Sometimes we use gases in the laboratory. These can be stored in gas cylinders (e.g. O₂), in Dewar flasks in liquid state (e.g. liquid nitrogen), or dissolved in water (e.g. HCl or NH₃). By working with gases it is very important to follow all safety instructions to avoid fire, explosions, frostbite or (in case of inhalation) asphyxia or poisoning.

Abstract

Mine spoils pose adverse conditions for soil microbes and plant growth, due to its low organic content and other essential nutrients, unfavourable soil chemistry and poor soil texture. Poor microbial population inhibits nutrient transformation, consequently the establishment of the plants and ultimately affects the process of ecological succession. A synergy among different groups of beneficial microbes accompanied by plantation of suitable tree species on mined over area has proved to be effective for speedy and efficient revitalization of the mined affected lands. Inoculation of plantation with efficient strains of native microbes accelerates the soil redevelopment and helps to establish nutrients cycle readily which attracts native flora thereby the pace of natural regeneration is increased. It is therefore imperative to consider the application of effective microflora in restoration of mined lands along with the plantation.

Aim

To synthesize gold (Au) nanoparticle using fungal mycelium.

Introduction

Biosynthesis of nanoparticles is a kind of bottom up approach where the main reaction occurring is reduction/oxidation. The fungal enzymes are usually responsible for reduction of metal compounds into their respective nanoparticles.

The history of nanotechnology in the modern world dates back to 1959 when American physicist Richard Feynman inspired the scientific community in his lecture “There is a plenty of rooms at the bottom”. Research on nanosized particles dramatically developed into an important field of modern research with potential effects in electronics to medicine and recently in agriculture and food industry.

Nanoparticles exhibit unique properties in terms of chemical, physical, photoelectrochemical and electronic properties when compared to their respective bulk materials. The applications of these nanosized particles are defined in a bigger ways in the field of Biotechnology. All these applications require custom based synthesis methods for specific applications.

Aim

To synthesize phosphorus (P) nanoparticles from tricalcium phosphate using fungal mycelium.

Introduction

Green nanotechnology includes a range of processes that reduce or eliminate toxic substances to restore environment. Microorganisms like fungi and bacteria having naturally bestowed property of reducing and oxidizing nanoparticles, are used for synthesis.

Introduction to the Salmonid Family (Trout’s and Salmon)
The Salmonidae constitute the only extant family of ray-finned fish in the order Salmoniformes, with 11 genera and more than 200 species collectively referred to as "salmonids" or "salmonioids". This family includes all coldwater mid-level predatory fish found in subarctic and cool temperate waters in the Northern Hemisphere, such as char, graylings, freshwater whitefish, taimens, lenoks, and salmon (both Atlantic and Pacific species). In addition to being the Exclusively Distributed by NIPA GENX Electronic Resources and Solution Pvt. Ltd. New Delhi eponym of its genus, the Atlantic salmon (Salmo salar), has also been given the names of its family and order as well. Among teleost fish, salmonids have a relatively primitive appearance, with pelvic fins in the rear and an adipose fin at the rear. There is a single row of sharp teeth in their mouth, along with rounded scales and forked tail fins (Mc Dowell, 1998). Salmonids vary greatly in size, and the smallest reach only 13 cm (5.1 in) when adults, while the largest reaches 2 m (6 ft 7 in) (Pauly et al., 2008). Salmonids are migratory fish that spawn in the shallow gravel beds of freshwater headstreams, develop into juveniles in rivers, creeks, small lakes, and wetlands, and then migrate downstream to spend the majority of their adult lives in larger bodies of water upon reaching maturity. Often euryhaline, salmonid species migrate to the sea or brackish estuaries as soon as they reach adulthood. Just for breeding purposes, they migrate back to the upper streams. Some freshwater salmonids that migrate only between lakes and rivers are referred to as potamodromous, whereas the life cycle of these sea-run salmonids is known as anadromous. In the middle food chain, salmonids are carnivorous predators that eat smaller fish, crustaceans, aquatic insects and larvae, tadpoles, and occasionally fish eggs (even those belonging to their own species), which are then eaten by larger predators. Salmonids are regarded as keystone organisms that contribute biomass to freshwater and terrestrial ecosystems as a result of their mass migration from oceanic to inland waterbodies. Three lineages of salmonids are currently recognized, each classified as a subfamily: Coregoninae (freshwater whitefish), Thymallinae (graylings), and Salmoninae (trout, salmon, char, taimens, and lenoks). Each of the three lineages is generally accepted to belong to a monophyletic group based on derived traits (McPhail and Strouder, 1997).
Salmons and trouts are well-known for their economic importance as both sport fish and commercial products. Trout and salmon stocks are stocked worldwide each year for sport and commerce. Millions of salmon and trout are hatched, raised, and stocked every year as part of government investment programs. To meet market and restaurant demands for trout, a large private industry has developed—especially in Denmark, Japan, and the U.S. Scientists are confronted with the challenge of perpetuating and increasing the abundance of salmon and trout because of the increasing human population and industrial and agricultural demands on rivers. Among heavily populated regions, the demand for trout outstrips the supply. As a result, state and federal agencies in the United States have initiated massive programs to raise and stock trout in heavily fished waters. The artificial abundance of trout, however, is not a good substitute for the natural abundance of the fish.

Introduction
Schizothoracinae, commonly known as snow trouts, are a specialized group of fish thriving in the fast-flowing, snow-fed streams and lakes of regions such as Afghanistan, Pakistan, the Himalayas in India and Nepal, China, and parts of the former USSR. Adapted to these challenging environments, Schizothoracinae have not been extensively studied due to the difficult terrain they inhabit. In India, they are primarily found along the Himalayan range, with an exception in the Periyar, Kerela (river and lake). The Indian Schizothoracinae are classified into seven genera, forming a significant part of Exclusively Distributed by NIPA GENX Electronic Resources and Solution Pvt. Ltd. New Delhi the Himalayan fishery. Resembling Salmonidae, they are commonly referred to as "Indian Trout." Early mentions of Schizothorax species by Day (1877, 1889) were brief. Subsequent taxonomic studies by researchers such as Hora (1921, 1934, 1937, 1953), Misra (1949), and Mukerji (1936) have provided a more comprehensive understanding of their taxonomy.
The Schizothoracinae, part of the Cyprinidae family, represent a diverse group uniquely adapted to high-altitude environments and have been categorized into three groups based on their morphological characteristics: "primitive group," "specialized group," and "highly specialized group" (Cao et al., 1981). The primitive group includes Schizothorax and Aspiorhynchus, which share more traits with the outgroup Barbodes hexagonolepis. They are distinguished by the presence of uroneuralia, three or four rows of pharyngeal teeth, minimal scale degeneration, and a lack of pronounced sexual dimorphism. The specialized group includes Ptychobarbus, Gymnodiptychus, and Diptychus, and is marked by the absence of uroneuralia, one or two rows of pharyngeal teeth, and moderate scale degeneration. The highly specialized group, which comprises Gymnocypris, Oxygymnocypris, Schizophygopsis, Chuanchia, and Platypharodon, is characterized by the complete absence of barbels and scales and a well-developed canalis preoperculomandibularis (Qi et al., 2012, Yonezawa et al., 2014). The genus Schizothorax comprises 63 species in total. Among these, 34 species are mainly found in Central Asia, while 28 species inhabit the Indo-Himalayan region. The species of Schizothorax in the Himalayan region include S. curvifrons, S. nasus, S. richardsonii, O. sinuatus, S. planifrons, S. esocinus, Schizothoraichthys progastus, S. longipinnis, S. kumanonensis, O. molesworthii, S. hugelli, S. labiatus, and S. micropogon (Menon, 1999; Mishra, 2003; Zhang et al., 2018; Ma et al., 2020).

The larval phase is among the most delicate and significant periods in the development of fish and shellfish. It represents the shift from the embryonic stage to early juvenile life and greatly influences survival, growth, and the overall productivity of aquaculture ventures. During this stage, the larvae exhibit distinct morphological, physiological, and behavioral traits, which differ considerably from those of adult organisms.
Newly hatched larvae are usually microscopic and underdeveloped, depending initially on internal energy reserves like the yolk sac for nourishment. After this endogenous feeding phase, they transition to feeding on external sources such as plankton, rotifers, and Artemia. Timely access to suitable food is crucial, as any delay can result in starvation and large-scale mortality.
Fish larvae undergo rapid structural changes as they develop, including the formation of fins, a functional mouth, and a digestive system. Likewise, shrimp and crab larvae progress through multiple stages—nauplius, zoea, mysis, and post-larva—each with specific needs for food and habitat.
Physiologically, larvae have high energy demands and poorly developed respiratory systems, making them extremely sensitive to environmental factors such as oxygen, ammonia, and temperature. Their weak immune systems and fragile exteriors make them prone to disease and stress from handling.
Larval feeding is influenced by visual and environmental cues, and due to their small mouth size, they require micro-sized live feeds early on. Hatcheries must, therefore, maintain live feed cultures and gradually transition larvae to formulated diets as they grow.
Effective hatchery practices depend heavily on an understanding of larval biology. Parameters like light exposure, stocking density, water conditions, and sanitation directly impact larval development. Even minor changes in temperature

1. Giant tube worms - Riftia sp.

2. Novel mussels Bathymodiolus sp.

3. The stock inside the marine protected areas is called as Bank account.

4. Cartilaginous fishes don't have swim bladder.

5. Non- endemic species - Grass carp.

6. Banned species - Tilapia.

7. Non-parasite fish - Echinoderms.

8. Non- Penacid fishery - Maharashtra.

9. Maximum shellfish and finfish exploiting state in India is Kerala.

10. Small reservoir more in Tamil Nadu.

I. INTRODUCTION

The fruits and vegetables we eat and flowers and ornamentals we enjoy are biological entities. They breath, produce heat, perspire, are easily damaged, get diseases and finally die. These carry on processes characteristics of all living things before or after harvest to perform the metabolic activities:

1. to maintain cellular organization
2. to transport metabolites around the tissue and
3. to maintain membrane permeability.

I. INTRODUCTION
The fruits and vegetables we eat and flowers and ornamentals we enjoy are biological entities. They breath, produce heat, perspire, are easily damaged, get diseases and finally die. These carry on processes characteristics of all living things before or after harvest to perform the metabolic activities:
1. to maintain cellular organization
2. to transport metabolites around the tissue and
3. to maintain membrane permeability.
The job of postharvest technologist is to understand life in fruits, vegetables and flowers and prolong their life as much as possible. The post harvest life of produce depends on the rate at which they use up their stored food reserves and their rate of water loss. The sugar and starches (carbohydrate derivatives) are stored in various parts of plants through a process known as photosynthesis occurring in growing plants and are used later to provide energy for further growth, development and to prolong post-harvest life. The process of photosynthesis in growing plants and respiration in harvested produce can be seen in Fig. 2.1. The released energy through process known as respiration occurs in plants before and after harvest. Thus, principal normal physiological processes leading to aging are respiration and transpiration.

Except lions, the tigers like other felids are highly solitary and however, during the breeding period, the males will approach the female for mating activity. Tigers are highly territorial in nature. Like other felids, retractile claws are present in felid which is an anatomically peculiar feature. They are crepuscular in nature and however, they are quite active in the morning hours.

Life cycle of nematode has six stages. The egg stage, first stage juvenile (J₁), second stage juvenile (J₂), third stage juvenile (J₃), fourth stage juvenile (J₄) and the adult stage.

The first four stages are the immature stages and are known as juvenile stages. The female lays eggs in soil or in plant tissues, singly or in groups as egg mass that hatch out into juveniles which are almost similar to adults appearance. The first moult occurs within the egg shell and the second stage juvenile comes out by rupturing the egg shells as J₂. In case of Xiphinema index, the juveniles are reported to emerge from the egg before the first moult. The juvenile cuticle is shed after each moult.

The egg

The nematode eggs are oval in shape. The eggs are covered by three membranes, the external protein layer which is the secretion of uterus wall, the middle chitinous layer or the true shell secreted by egg itself and the inner lipid layer (vitelline layer). The chitin content in the egg shell vary in different species of nematodes.

Life cycle of nematode has six stages. The egg stage, first stage juvenile (J1), second stage juvenile (J2), third stage juvenile (J3), fourth stage juvenile (J4) and the adult stage.

Introduction

The biology is the study of life forms, subdivided into a variety of specialist categories that include their morphology, physiology, anatomy, behavior, origin, and distribution.The conservation of endangered species is often equated with the preservation of biodiversity by people all over the world. Although endangered species are among the most obvious and understandable markers of the continual loss of biodiversity, ecologists are aware that biodiversity involves much more than just endangered species. An increasingly popular and crucial component of initiatives to preserve the ecological diversity of the planet is the protection of such species.

Endangered species

Endangered (EN): A taxon that is not critically endangered but facing a very high risk of extinction in the natural environment in the near future, it is considered to be endangered.

The important criteria are

1. A decline of at least 50% over at least 10 years or three generations that has been observed, estimated, inferred, or suspected. 2. Population thought to consist of no more than 2500 mature individuals.