eChapters

55.1. Aim

To determine dietary fibres in given samples.

55.2. Theory

Fibres can be classified into four categories like crude fibre (cellulose); acid detergent fibre (cellulose + lignin); neutral detergent fibre (cellulose + lignin + hemicelluloses) and total dietary fibre.

55.3. Procedure for estimation of dietary fibre

55.3.1. Acid detergent fibre (ADF)

Reagents: Cetyl tri-methyl ammonium bromide (CTAB) reagent and Acetone

Apparatus: Digestion apparatus vacuum pump, oven, desiccator and sintered glass crucible.

54.1. Aim

To determine crude fibre in given samples.

54.2. Theory

Fibres can be classified into four categories like crude fibre (cellulose); acid detergent fibre (cellulose + lignin); neutral detergent fibre (cellulose + lignin + hemicelluloses) and total dietary fibre (Cellulose + lignin + hemicelluloses + pectic substances). Crude fibre is the organic residue of a sample. A fat free sample is treated with boiling sulphuric acid and subsequently with sodium hydroxide. The residue after subtraction of ash is regarded as crude fibre.

54.3. Reagents/ Apparatus/Glassware

1. 0.255 N Sulphuric acid solution

2. 0.313 N Sodium hydroxide solution

3. 10% Potassium sulphate solution

4. Ethanol

26.1. Aim

To determine the pH of food samples.

26.2. Theory

The hydrogen-ion concentration of a food is a controlling factor in many chemicals and microbiological reactions. Pure water has equal concentration of H+ and OH- ions, each having the value of 1×107g/litre at room temperature that is why water is regarded as neutral. The pH of pure water is 7.0, the solution having pH below 7.0 is regarded as acidic while solution with pH above 7.0 is alkaline. Most fruits and fruit products have pH below 4.0 while vegetable; milk and meat products have pH more than 4.0. The pH value of some fruits and fruit products is given in Table 6.1. Thus, with the increase in acidity in the solution, the pH value decrease and vice versa. However, presence of buffer salts helps in maintenance of pH. Estimation of pH of the food is important as the processing conditions for different food products are categorized on the basis of their pH.

23.1. Aim

To determine the total soluble solids in fresh and processed fruit and vegetable products.

23.2. Theory and principle

Total soluble solids (TSS) are determined by means of brix which measures the specific gravity or by using hand refractometer which measures refractive index. Brix is a measure of soluble solids only in case of pure sucrose solutions. Generally, the fruit juices contain more sugar than any other soluble constituent. Hence, brix provides a useful guide of soluble solids or sugar contents. Brix hydrometer is used to determine the percentage of brix in syrup while Abbe refractometer gives refractive index as well as degree brix. The Refractive index of the test solution is measured at 20°C using a refractometer and the soluble solid content is determined by the use of tables correlating refractive index with soluble solids. Generally, hand refractometer is used for determining TSS in food samples in fruit processing units (Table 3.1).

2.1 Introduction

Greenhouses are used mainly for cultivating vegetables and fruits during the off-season to provide favorable growing conditions. Proper management of the greenhouse structure is necessary to create these off-seasonal favorable conditions for plants. Factors like site selection, location, atmospheric conditions, cropping pattern, and structure of the greenhouse are considered during the development of a greenhouse. The National Committee on the use of Plastics in Agriculture (NCPA-1982) suggests conducting site-specific greenhouse technology trials before implementation across the nation.

1. Introduction

Jackfruit is a fruit crop that is under appreciated and neglected. The jackfruit (Artocarpus heterophyllus Lam.) is a member of the Moraceae family. India is the world’s greatest cultivator and producer of jackfruit, also known as the poor man’s fruit because it grows more in rural than metropolitan areas. It is a pretty huge tree that bears the world’s largest edible fruit. The jackfruit is a tropical fruit native to Southeast Asia. It is thought to have originated in India’s rainforests, in the Western Ghats, and is now widely farmed throughout the region’s lowlands. The leading jackfruit producers in the world include Bangladesh, India, Myanmar, Nepal, Thailand, Vietnam, China, the Philippines, Indonesia, Malaysia, and Sri Lanka. Jackfruit can be found in East Africa, Brazil, and Caribbean nations such as Jamaica. Bangladesh’s national fruits are the jackfruit, mango, and banana.

New varieties of bougainvillea have been developed in India at different research institutions, nurseries, botanical societies and also by individual efforts. Details have been mentioned above. New varieties developed at CSIR-NBRI need special mention. 

CSIR-NBRI has developed a number of new varieties by using various available breeding tools viz. through spontaneous mutation, classical breeding, chromosome manipulation and induced mutation (Banerji 2007, 2008, Banerji and Datta 1986, 1988, 1993, 1996, Banerji et al., 1987, Data and Banerji 1990, 1994a,b, Datta 1992b, 2004, Nath et al., 1983, Ohri 1995, Sharma 1996, Zadoo et al., 1975b, Jayanti et al., 2007, Roy et al., 2015).

Dietary constitution of Indian people is mostly cereal based. However, the cereals supply chiefly carbohydrates. Therefore, there is deficiency of protein, minerals and vitamins, especially vitamin A, riboflavin, vitamin C, etc. in the diet. The dieticians advocate the intake of 125 g leafy vegetables, 75 g other vegetables and 100 g root and tuber vegetables (total 300 g) everyday because vegetables provide all the nutrient components like carbohydrates, protein, fat, minerals, vitamins and water along with roughages, which are the essential constituents of a balanced diet.

India has attained food security by increased cereal production due to green revolution. In case of vegetables, although there has been increase in production from 15 million tonnes during 1950 to 94 million tonnes in 2005 but still more vegetables are needed to meet the minimum requirement of at least 250 g per day per capita. There has also been increasing demand for fresh export and for processing industries. Moreover, there is a pressure for increasing vegetable supply caused by constantly increasing population in a world of limited acres.

Addition of new agricultural land is of course not possible. In this situation, plant breeding can be expected to contribute substantially to greater vegetable productivity. This can be accomplished not only by the breeding of basically higher yielding varieties but also by the development of varieties that help to stabilize production through resistance to various biotic and abiotic stresses.

All over the world, garden designing has a historical back ground. Every continent has a definite style based on cultural heritage, agro-climate and the rulers. There are influences of one style over the other and subsequent change in gardening style also. However, there are some recognized orthodox styles like ‘English Garden’, ‘French Garden’, ‘Japanese Garden’, ‘Italian Garden’, ‘Dutch Garden’, ‘Mughal Garden’ etc. These are unique and true to their specific characters. Modern gardens all over the world have been greatly influenced by all these styles.

History and characteristics of these gardens are given below in order to make one acquainted with these styles.

Dutch Garden

Initially, gardening in The Netherlands was started in the form of abbey and monastery gardens (middle ages 500-1500 A.D.). Design of the medieval gardens usually was elongated rectangle, sometimes square, also divided with cross paths. These gardens were mainly meant for production of vegetables, medicinal herbs and fruits besides flowers.

During the Dutch renaissance (1500-1650 A.D.) further gardening activities initiated. After independence in 1609, it gained momentum and the change of garden style also noticed. Gardens became more open without walls. However, the basic design was on the basis of geometrical patterns - rectangular divided into small separate square. Axis of the garden was not in relation to the building. Leafy walk path (berceaux) was main structural elements created between various sub-divisions. Bulbous plants specially tulip was most preferred in the garden besides marigold, carnation, poppy, rose etc.

Digital technologies are transforming horticultural production by enabling data-driven crop management and precise decision-making. This chapter introduces agricultural informatics, sensors, Internet of Things, remote sensing, drones, artificial intelligence, machine learning, geographic information systems, robotics, and precision technologies. These tools can support crop monitoring, disease detection, irrigation scheduling, nutrient management, yield prediction, phenotyping, and resource optimization. AI-based image analysis can identify symptoms and assist in early crop diagnosis, while sensors provide continuous information about soil and plant conditions. The chapter emphasizes the integration of digital data with practical horticultural knowledge to improve productivity, profitability, sustainability, and resilience while reducing unnecessary inputs and improving decision-making.

Introduction
Dill seed/sowa belong to the genus Anethum and it is derivative of Greek word ‘aneeson or aneeton’, meaning thereby strong smell. The botanical name of dill seed is Anethum graveolens L. It is the only species falling in the genus Anethum, and known as East Indian dill or Sowa (Anethum graveolens var. sowa Roxb. ex, Flem.) in India. It is cultivated as a foliage crop in rabi season stretched in the Indian subcontinent.
Origin
It seems to be originated in South-east Europe or South-west Asia and also native to Central Asia, Mediterranean and southern USSR. It belongs to India, as it is found growing in the states of Maharashtra, Gujarat, A.P., M.P. and Rajasthan.
Uses
• Used as in abdominal discomfort, colic and promoting digestion.
• Cures ‘vata’, ‘kapha’, ulcers, eye diseases and uterine pains.
• Paste of dill seed with milk is used for external use in rheumatic and swellings of joints.
• Attributed to tonic, rejuvenating and intellect promoting properties.
• Used as condiment.
• Seeds are used as carminative, aromatic, galactogogue, mildly diuretic, stomachic and stimulant.

1. DISEASES OF MANGO

Mango is considered to be the king of fruit. India is the largest producer and exporter of mango in the world. Mango possess unique nutritional and medicinal qualities apart from being a rich source of vitamins A & C, besides its attractive form and appearance, delicious taste and appetizing flavor, the ripe mango fruit according to nutritional experts is also highly invigorating, fattening, laxative and diuretic. Every part of mango from root to tip is used in a variety of ways. This crop is affected by many fungal, bacterial and other non parasitic diseases.

1. Mango Malformation Disease (MMD)

Mango Malformation Disease is a fungal disease of mango caused by several species of Fusarium, some yet to be described. Mango is the only known host of the disease. The disease spreads on a tree very slowly, but if left unchecked, can severely reduce yields. The main method of spreading MMD to new areas is through infected vegetative planting material. There is no evidence that the disease can spread on fruit or the seeds, or that it affects human health. It usually associated with the bud mite, Aceria mangiferae but the mites have been shown to spread the disease within a tree and not between the trees. Mango malformation, also known as bunchy top, is a very serious threat to the mango industry, particularly in northern India. The Etiology of the disease still remains obscure and diverse claims have been made about its causes, e.g., physiological, viral, fungal, acarological and nutritional.

Importance of fruits in human diet is well recognized since time immemorial. They are important part of a healthy diet as natural sources of energy, essential vitamins, minerals and antioxidants ever since the beginning of humancivilization. Our country is bestowed with varied climatic conditions, which had made it possible to grow diverse group of fruits from its temperate to tropical, to subtropical and even to arid niches. The cultivation of fruit crops is, presently, one of the most important and profitable proposition. Growing fruit crops translate into realization of more yield/ income from a unit area of land than any of the agronomic crops. However, there is need to considerably increase the yield of fruit crops, particularly under changing environmental conditions as under such conditions diseases are getting more prevalent. Therefore by adoption of proper protection practices, yield of fruit crops can be improved.

Plant diseases caused by fungi, bacteria, viruses and phytoplasmas reduce crop yields (Khoury and Makkouk, 2010).Adoption and support for using participatory approaches help to farmers in disease management of fruit crops, reducing costs and improving production efficiency. Fungal, bacterial and viral diseases pose serious threat in the cultivation of fruit crops in India. To reduce the losses caused by plant disease, a complete knowledge of disease management strategies of fruit crops are essential which are as follows:

Introduction
Vegetable crops are highly susceptible to biotic stresses indicated by disease causing micro-organism (fungi, bacteria, virus and phytoplasma). Indiscriminate use of chemicals for the control of diseases, insect pest have
several disadvantages, particularly increased cost of cultivation, residual toxicity in food chain and development of resistance against pathogen. Management of diseases involves use of resistant varieties and integration of cultural and chemical control measures. Of the biotic stresses much of the breeding efforts have been directed towards developing resistance against diseases. In fact, breeding for resistance, has been one of the most important objective of vegetable breeder in the last half of the century. Genes that contribute to pathogen tolerance/resistance can be obtained from local germplasm resources, or through exotic lines, wild species or genera, or lines from other breedingprograms. In the biotechnological approaches for vegetable breeding also,  most of the achievement has been to generate transgenic plants resistance to disease. Often breeding for resistance has assumed greater importance in a number of vegetable crops.
Major Diseases of Tomato
Early blight: The casual organism is Alternaria solani. The symptoms appears as small, black spots with enlarged and concentric rings in a bull’s eye pattern in the center of the leaves. Tissue surrounding the spots may turn yellow. On fruits, dark brown concentric rings are seen that affects the market quality.

1. Introduction

Eggplant or brinjal (Solanum melongena L. family. Solanaceae) is the major vegetable crop of wet and hot climatic zone of south Asia (Srinivasan and Huang, 2008). After China, India is the second largest brinjal producer country in the world (Majumdar and Das, 2020). It is nutritious vegetable and consumed in throughout the world. Brinjal is considered as Indian native crop and it has been cultivated for last 4000 year. India is known to be the Origin Centre of brinjal cultivation, from where it distributed to throughout the world (Choudhury and Malda, 1968). In India, brijnal crop grow almost throughout the year. Brinjal fruit contains moisture 92.7g, carbohydrates 4.0g, proteins 1.4g, minerals 0.3g, vitamins 124 I.U. and vitamin 12.0mg per 100 g of edible part (Kalra et al., 1988). It is used as vegetable purpose as well as pickle (Singh et al., 1963).

The arid zone of India covers about 12% of the country’s geographical area and occupies over 31.7 mha of hot desert and about 7 m ha under cold desert. The arid regions of Rajasthan, Gujarat, Punjab and Haryana together constitute the Great Indian Desert known as Thar Desert that accounts for 89.6% of hot arid regions of India. In Gujarat, 6.22 m ha area is under arid zone which constitute 19.6% of the arid area of country (Fig 1). In Gujarat, eight districts fall under arid zone, namely, Kachchh (100% of district area), Jamnagar (80%), Surendranagar (29%), Junagadh (20%), Banaskantha (18%), Mehsana (7%), Ahmedabad (6%) and Rajkot 6%).

Turmeric (Curcuma longa L.) is a tropical herbaceous plant extensively cultivated in India, Sri Lanka, parts of China and Pakistan.  Country of origin is not known with certainity, it is either of Chineese or Indian origin. India ranks first in area (1.49 lakh ha.) and production (5.27 lakh tonnes.) in the world and it is grown in most of the states in the country.  Major turmeric growing states are Andhra Pradesh (56,000 ha.), Orissa (23,000 ha.), Tamilnadu (17,000 ha.), Assam (12,000 ha.), West Bengal (11,000 ha.) Maharashtra (6,000 ha.)  Karnataka (6,000 ha.) and Kerala (3,000 ha.).  Andhra Pradesh ranks first in area and production in the country. Area under this crop is increasing in North Eastern States.

Turmeric belongs to family Zingiberaceae and genus Curcuma. Genus Curcuma has 42 species, of which Curcuma longa is extensively cultivated for culinary purposes, Curcuma aromatica for flavour and recently Curcuma amada (mango ginger) for preparation of chutneys. Turmeric is mainly cultivated for under ground rhizomes, rich in curcumin. Turmeric is traditionally used for medicinal, religious, culinary purposes and also as a cosmetic and dye (Shah 1997). Recent reports in controlling human cancer has given renewed interest on the crop. In Ayurveda, turmeric is regarded as aromatic, a stimulant tonic, carminative and anti helminthic. Essential oil of turmeric is antiseptic and is used in treating gall stones and gall complaints (Pruthi 1976).

Horticulture is a wide field and includes a great variety and diversity of crops. In general, these crops are classified as follows:

1. Crops grown for the food and/or vitamin and mineral content, for e.g., apple and sweet potato.

2. Crops grown for their beverage properties, for e.g., tea and coffee.

3. Crops grown to beautify the environment, for e.g., the grasses of the well planned lawn.

4. Crops grown to express sentiments, for e.g., cut roses, jasmine.

5. Crops grown for perfume for e.g., rose, jasmine.

6. Crops grown for spices for e.g., pepper, nutmeg.

A laboratory is essential for research work in science and development. Most of the research institutes have dedicated laboratories for researchers and students to conduct experiments, perform research work, as well as to provide hands-on learning/training to students. There are certain rules and regulations laid down by the laboratory in-charge which need to be followed strictly for safety purposes. Some of the laboratories keep ‘safety manual’ for researchers to read before entering into the laboratory to conduct experiments. Whenever safety is compromised in a laboratory, there is increase in risk of unwanted incidents in the laboratory. Storing food/beverages in the same refrigerator where laboratory chemicals are kept may lead to disastrous effects on health. This chapter aims to provide instructions in brief on the things to be performed (Do’s) before and after entering onto a laboratory as well as the things never to be done (Don’ts) in a laboratory for the safety reasons.

Good Laboratory Practices (Do’s)

• P Wear apron/labcoat while working in the laboratory.
• P Use gloves/respiratory mask/fumehood while handling toxic/volatile/inflammable chemicals/reagents.
• P Use pippet or pippetor for safety in transfer of solutions.
• P Protect eyes, face and naked parts of the body from UV radiations. Use safety goggles or face mask/apron.
• P Keep your eyes away from Liquid Nitrogen and use cryogloves while handling it.
• P Arrange all the necessary materials/solutions before starting the experiment.
• P Periodically fumigate/ozonize culture and inoculation rooms.
• P Autoclave all contaminated materials before discarding.
• P Pre-treat gel or solution containing Ethidium Bromide (EtBr) before discarding into laboratory waste-disposal bin, and use gloves while handling them.
• P Burn all the transgenic plant materials in an incinerator after taking necessary observations.
• P Make entry in the designated log-book and clean the instrument after using it properly.
• P Switch-off the instrument after use or when not in use.
• P Wash hands with soap and remove the labcoat before leaving/going out of the laboratory7.
• P Ensure that switches for fans/lights, and water taps are off while leaving the laboratory.
• P Follow the biosafety guidelines suggested for handling and disposal of the hazardous chemical.

In literal sense a species is called underutilized whose potential has not been properly realized. These underutilized crops (referred to also by other terms such as minor, orphan, neglected, underutilized, underexploited, underdeveloped, lost, new, novel, promising, alternative, local, traditional, niche crops) have been included in world-wide plans of action after having successfully raised the interest of decision makers. Wealth of plant genetic diversity currently available holds vast potential. We use only small number of crop plants for our basic requirements. Many thousands of wild plants have great economic and cultural importance and tremendous market potential for vast numbers of people throughout the world. But the majority are still unknown to science. Plant genetic resources have gained global attention in  recent past by agricultural and environmental institutions. Conservation and use of genetic resources attracted  considerable attention. Value of biological diversity in economic terms is very important for human society.