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The importance of green manuring in crop production has been known since ancient times. However, awareness towards soil enrichment is increasing in recent years, mainly due to comparatively high cost of inorganic fertilizers, increased risk of environmental pollution and need of sustainable cropping systems. Green manuring helps to improve physical, chemical and biological properties of soil and consequently crop yields. Green manuring lowers the nitrogenous fertilizer requirements for succeeding crops. However, it’s influence may vary with soil, crop environmental variables, type of green manure crop used and its management.

Green manuring is a practice of ploughing or turning into the soil un-decomposed green plant tissues for the purpose of improving physical structure as well as fertility of the soil. It is obtained in two ways; by growing green manure crops or by collecting green leaf (along with twigs) from plants grown in wastelands, field bunds and forest. Green manuring by growing the field plants usually belonging to the leguminous family and incorporated into the soil after sufficient growth. The most important green manure crops are sunnhemp, dhaincha, Pillipesara, cluster beans and Sesbania rostrata. The advantages of green manuring are as follows.

Growth and development are two important stages in a plant. Plant growth and development are under the control of both intrinsic and extrinsic factors. Growth is one of the fundamental characteristics of all living things. Even in Plants, the growth is visible but not measurable easily. Development in plants is actually a sum of two processes i.e. growth and differentiation.

Principles involved in plant growth and development: The various principles involved in plant growth and development are discussed below.

1. Totiopotency: Living plant cell has the potential to reproduce all the characteristics of the plant since it possesses all the genetic information.
2. Competence: It is the potential of cells or tissue to develop in a particular direction. Development of competency into a particular kind of cell(s), tissue or organ also may require special signals. These may originate internally within the plant or may originate externally as an environment signal, e.g. Internal change in type of growing point such as vegetative to flowering or initiation of adventitious roots on cuttings may be associated with a shift in the activity of specific hormones, external parameters i.e. application of growth regulators, or environment. It is mainly internal potential of meristem that gradually shifts from a vegetative shoot to one that produces flowers.

Guava (Psidium guajava L.), belonging to family Myrtaceae, is cultivated and naturalized in the tropical and subtropical regions of the world. It occupies fifth place in area (0.15 million ha.) and fourth place in fruit production (1.80 million tonnes) in India. It has gained considerable prominence owing to its high nutritive value, availability at moderate price, a pleasant aroma and good flavour. Its fruits are used for both, fresh eating and processing. It excels most other fruit crops in productivity, hardiness, adaptability and vitamin C content. Besides its high nutritive value, it bears heavy crop every year and gives handsome economic return involving very little inputs. This has prompted several Indian farmers to take up guava cultivation on a commercial scale (Chadha, 2003). Guava trees are not difficult to grow and can survive in a range of soil and climatic conditions. However, precise management is required to produce a highly profitable crop.

Origin and history: Guava (Amrud) is one of the most common fruits in India.  It is a very hardy sub-tropical plant and prolific bearer. In India, it is commonly called as “poor man’s apple” widely naturalized in the country. Allahabad has the reputation of growing the best guava in the country as well as in the world. Area wise guava comes under 4th position after Mango, Banana, Citrus, in India. Uttar Pradesh has the largest area fallowed by Bihar, Madhya Pradesh, Maharashtra, Gujarat, and West Bengal, however it is generally grown all over India and also grown in South Asia that is Srilanka, Myanmar and tropical and subtropical country. Beside Psidium guajava some other important species which are commercially grown for rootstock purpose that is :

Gypsophila is herbacaeous annual or perennial flowering plant belongs to family Caryophyllaceae. It contains several ornamental species of which Gypsophila paniculataL. is the only species used as a cut flower (Zvi et al., 2008) and it is applied as filler in flower arrangements (Jones, 1995 and Rehman, 2002). Gypsophila derived from “gypson” meaning chalk and “phylos” meaning lover, an etymology which alludes to its preference for calcareous soils (Vettory et al., 2013). Mainly found in temperate or warm-temperate regions in the Northern Hemisphere, best developed in the Mediterranean region and Near East (Ataslar et al., 2009 and Cronquist, 1981). It is a perennial plant often grown commercially as an annual crop. Root is rich source of triterpine saponins (Henry, 1993, Hostettmann, 1995 and Gevrenova et al., 2010) which are used for commercially in medicines, detergents, adjuvents and cosmetics (Hostettmann, 1995, Tschesche and Wulff, 1973 and Gevrenova et al., 2010). G. paniculata is one of the ten best selling cut flower species worldwide and thus becomes for the genetic improvement and selection of new varieties of commercial interest. Main regions of gypsophila production are Italy, Israel, Belgium, Germany and Holland while currently its cultivation is spreading across to other countries such as Ecuador, Egypt and Kenya due to lower labour costs. Infact, Gypsophila is among one of the most important varieties of cut flowers in Israel, where it is grown in more than 200 hectares of greenhouses and is almost entirely exported. For these reasons, the competition has increased dramatically, making the world market increasingly difficult for companies which feel the need to introduce new varieties that respond better to characteristics desired by consumers and florists.

Introduction

Use of acid/base is common in laboratories for experiments like titration and proper safety in their handling laboratory is necessary. In this chapter we will briefly discuss about the safety measures necessary to be adopted while using acids/bases in laboratory for any experiment.

Handling of Strong Acid/Base

There are four major points that need to be taken care of while working with strong acids or bases that might be quite harmful if any accident happens.

1. Protect Yourself

Wear a lab-coat and acid-resistant gloves: Always use lab-coat/apron and make it sure that the sleeves properly cover your arms. More importantly, use gloves made of acid-resistant materials and also wear shoes to protect your feet while wearing long-pants/jeans. Female with long hair, must tie her hair properly (with rubber band) to avoid any disturbance caused by appearance in between.

Wear safety goggles: It is important to wear safety goggles to protect your eyes using larger and fitting goggles that cover front as well as sides of your eyes. Always use properly fitting and adjustable goggles because smaller/ loose goggles may not protect the areas around your eyes against accidental acid spills and it may fall down from your face, if it is too loose.

Locate emergency wash area: There must be an emergency washing area nearby the work place or laboratory containing lab spill management kits. All the workers in the laboratory must be aware of using the kits and emergency plans.

Neutralize acid: Don’t panic even if an acid or chemical spill happens. Stay calm and act as quickly as possible. In case an acid spills, neutralize it by using sodium bicarbonate so that they can be cleaned and disposed safely. If acid comes in contact of skin, wash it off under running tap water but do not use/ apply sodium bicarbonate on skin.

Introduction

For proper and efficient experiment to be conducted, experimenters need so many chemicals to deal with. This includes different acids, base, salt, different kind of indicators and so on. Some of these chemicals are safe for health, but some of them are dangerous to health when come in close proximity to our body while some of the chemicals cause health issues on inhaling. These may cause improper functioning of some of our physiological activities. Acids and bases are very dangerous for our tissues when come in our contact. These may cause serious respiratory problem in addition to injuries on coming to our contact. Some chemicals are exploratory yet they may cause even death of the person working with them in laboratory. Therefore, mere use of the chemicals in laboratory is not only the objective of the experimenter. The person working in laboratory should also be concerned about the proper use and handing of the chemical while working with it. Thus, every researcher of an institution who has to work with chemicals in a laboratory should be made aware about the chemicals to be used in the laboratory and the consequences if any mishandling of a laboratory chemical occurs. Not only these, suitable precautionary measures should also be known to the researcher along with ensuring the availability of the required safety equipment/installations. Hence, a proper understanding of the ways of handling of chemicals in a laboratory is of prime importance for the personals like you who are working or have to work in laboratory.

Safe Handling of Chemicals in Laboratory

Safe use/handling of chemicals in laboratory include minimized exposure to chemicals, maintaining safety data sheets, proper labelling, storage, segregation, and transport of chemicals for personal hygiene.

Introduction

We come across a variety of chemicals every day in a laboratory. While some of them are relatively safer, others are extremely hazardous. Thus, it is very important to know the potential hazards that can be caused by the chemicals present in the laboratory and the safety measures needed to be followed to avoid the hazards. Additionally, we should also be aware of the steps necessary to be taken on such hazardous situations. According to IUPAC, a hazardous chemical mean the chemical which has the potential to cause damage in various ways. In a laboratory, there are chemicals that might be irritant, carcinogen, respiratory fibrogen, systemic poison, asphyxiant, flammable, etc. which have the potential to cause hazard to the workers or environment. Therefore, it is important to use appropriate procedure to reduce the risk and protect the health/safety of laboratory personnel, the public and the environment.

Types of Hazards Associated with Chemicals

Chemical hazards: The hazards caused by chemicals can be broadly classified into three categories.

1. Health hazard: This is also known as toxic effects of chemical. The risk associated with a chemical in laboratory depends on the extent of exposure and the inherent toxicity of the chemical. Many chemical reactions form the product that might be much more toxic than the reacting chemicals. For example, inadvertent mixing of formaldehyde (a common tissue fixative) and hydrogen chloride results in the generation of bis(chloromethyl) ether, a potent human carcinogen. For most of the chemicals, the route of exposure (through skin, eyes, gastrointestinal tract, or respiratory tract) is more important (Figure 17.1) that must be considered during risk assessment.

Plant tissue culture is mainly referred to as mass-multiplication of in vitro derived plantlets grown under controlled environment. Subsequently, the production of artificial seeds from in vitro grown plant propagules is also a method of mass-production of true-to-type plantlets. Micropropagation allows rapid and mass production of high quality, disease free and uniform planting material irrespective of the season and weather. Micro-shoots on being transferred to ex vitro conditions are exposed to abiotic (altered temperature, light intensity and humidity conditions) and biotic stress conditions i.e. soil micro-flora, so there is need for acclimatization for successful establishment and survival of plantlets (Deb and Imchen, 2010). In order to increase growth and reduce mortality in plantlets at the acclimatisation stage, research has been focused on the control of the environmental conditions (both physical and chemical) and to acclimatize the plants to compete with soil microflora (Mathur et al., 2008). In this chapter methods of acclimatization of plantlets derived from simple plant tissue culture or from synthetic seeds are discussed.

It is not just enough to produce fruit and ornamental plants in a nursery, but it is also very much important to consider the post production management practices like harvesting, packing, storage and marketing of nursery plants for successful industry.

Harvesting: Harvesting is one of the important factors considered, when nurserymen are producing seeds, bulbs, etc. The seeds or bulbs should be harvested at proper maturity stages depending on the plant species.

Seeds are generally harvested when they are fully ripe. In some of the annuals and perennials, seeds are likely to fall from the plant at ripening either due to bursting of the fruit/ capsule or due to wind. (calendula, balsam). In such cases, it is better to cover with muslin cloth or butter paper bags. Bulbs, corms and tubers are carefully dug out without injury, along with cormels or bulblets.

Packaging: The package should provide a convenient mode used for marketing and distribution. It protects the contents from undue damage during distribution. It is important that appropriate packaging should be used to preserve quality of material along with maximum survival during transit. Proper packaging of plant material (fruit plants/ ornamental plants/seeds) ensures quality during storage, distribution and transport.

• Turkish hazelnut production of 625,000 tons accounts for approximately 75% of world productions.
• The common hazel (Corylus avellana) is native to Europe and Western Asia.
• In H P, it is found growing wild in Pangi region of Chamba district and locally known as Thangi.
• Hazelnut is also known as cobnut and filbert.
• Hazelnuts are extensively used in confectionery to make praline and also used in combination with chocolate truffles.
• The temperature of minus 10°C is critical, especially if accompanied by wind, which may kill both pistillate and staminate flavours.
• Hazelnuts are more shallow-rooted, most fruit and nut trees do not tolerate wet soils, the tree cannot tolerate excessive dry summer heat and hot winds.
• Hazel will grow in pH ranging from 4.5-8.5 but around pH 7 is ideal.

Heliconia belongs to family Heliconiaceae is popular as ornamental plants and cut flowers because of their brilliant colours and exotic appearance. The genus name “Heliconia” is derived from “Helicon”, a mountain in southern Greece regarded by the ancient Greeks as the home of the Muses, thus suggesting the relationship between these plants and the bananas, genus Musa (Berry and Kress, 1991). Unique features of the Heliconiaceae are (1) medium to large erect herbs rising from underground rhizomes;(2) each erect shoot is composed of a stem and leaves, whereas the stem is made up of an axis covered by overlapping sheathing leaf bases, technically called a pseudostem;(3) inverted flowers and (4) the presence of a single staminode. Heliconia has long been popular horticulturally because of their showy inflorescences. It was so attractive that early explorers of the tropics returned to Europe with several species that became prized green house specimens. Heliconia, grown in nearly all of the tropical regions of the world, including Africa and Asia. In India, heliconia is grown in North-East region and Kerala.

ORIGIN AND DISTRIBUTION

Heliconias are native only to Central and South America and some of the islands of the South Pacific, for horticultural and commercial popularity. Berry and Kress (1991) stated that they are native from Tropical America and found at different altitudes, from sea level up to 2,000 meters in Central and South America, and up to 500 meters in the South Pacific Islands (Criley and Broschat, 1992).

Most of the Heliconia species are distributed primarily in Neotropical areas from the North of Mexico to the South of Brazil (Santos, 1978, Dahlgren et al., 1985 and Kress, 1990). A small Paleotropical group, about eight species, occurs in islands of the South Pacific (Tomlinson, 1969 and Kress, 1985). There are approximately 40 Brazilian species with two primary areas of distribution: the Amazon basin and the Atlantic coastal forest (Kress, 1990). About 100 or more species, native mainly to tropical and subtropical South and Central America (Cronquist, 1981).

Several attempts have been made over the years to sort out the taxonomy of the Old World Heliconia. Baker (1893), followed by Schumann (1900) and Winkler (1930), dealt with the problem of identity and origin of these taxa by assigning them to the neotropical species Heliconia bihai L. Others such as Ridley (1908a, b), Backer (1920) and Green (1969) recognized the distinctive and endemic features of the paleotropical taxa that separated them from the neotropical species. This ornamental rhizomatous herbaceous plant from the Heliconia genus, belongs to the Musaceae family, now constitutes the Heliconiaceae family in the Zingiberales order. The various species of Heliconia are subdivided into five subgenera: Heliconia, Taeniostrobus, Stenochlamys, Heliconiopsis and Griggisia; and 28 sections (Kress et al., 1993).

INTRODUCTION

Heterosis has contributed significantly towards increased crop production and it has become the basis of multibillion dollar agro-businesses in the world (Phillips, 1999). Hybridization is the important way that can create genetic variability and make new options. The F₁ population is obtained from the crossing of two homozygous but genetically dissimilar gametes or individuals (purelines in self pollinated crops or inbred lines in cross pollinated crops) that shows increased or decreased vigour over the better or mid parent value. Heterosis is maximum in F₁, generation which diminishes gradually in later generations. Hence, production of F₁, hybrid seeds of different flower crops is considered as the extreme focused area in floriculture industry.

Production of F₁ hybrids in ornamental crops was carried out even before Shull’s classical theory of hybrid vigour which was reported in 1971 for the first time in plant breeding. Benary Seed Company in Germany released F₁ hybrid in begonia (Begonia semperflorens) in the name of Prima Donna in 1909 (Reimann-Philip, 1983). But that time it did not attract the attention of plant breeders. It was during Second World War in 1942, when flower breeders were attracted towards heterosis breeding and started systematic F₁ hybrids. From 1950 onwards, numerous outstanding hybrids have been developed in many other flower crops in several countries like USA, The Netherlands, Denmark, Germany, United Kingdom and Japan, etc. First hybrid in single geranium was produced in 1960, in other flower crops like antirrhinum, pansy, marigold and zinnia in 1965, ageratum in 1966, geranium double in 1970, dianthus, begonia, impatiens and portulaca in 1976-77, gerbera in 1980 and carnation in 1981. In the present scenario of developed floriculture industry, in many countries F₁, hybrids are available in a number of annual flowers like ageratum, antirrhinum, anemones, calceolaria, dianthus, hollyhock, gazania, marigold, flowering tobacco, pansy, portulaca, stock, wall flower, zinnia, etc.

As emasculation is having the maximum share in cost of hybrid seed production, researchers have found several techniques in different flower crops as the alternative of emasculation and that can help in reducing the cost of hybrid seed production. The list of techniques being utilized commercially in different flower crops (Janakiram, 2004) is as follows:

Hybrid vigour is used as a synonymous of heterosis. In 1910, the term heterosis was first used by Shull who defined this phenomenon as the stimulus of heterozygous, and in his word, it has been the “interpretation of increased vigour, size, fruitfulness, speed of development, resistance to insect-pests and diseases or to the climatic rigors of any kind manifested by the out breeding organism as compared with corresponding inbreds as a specific result of the unlikeliness in the constitution of uniting parental gametes”. This definition is often interpreted as not implying a genetic basis for heterosis, because the definition basically describes the phenotypes that result from the crossing of two different inbred lines. Generally, heterosis is manifested as an increase in vigour, size, growth rate, yield, or some other characteristics but in some cases, the hybrid may be inferior to the weaker parent. This also represents as heterosis (average heterosis), and if heterosis is estimated over the superior parent, such an estimate is sometimes referred to as heterobeltiosis, hence, authors feel “heterosis refers a biological phenomenon in which the F₁ population obtained by crossing of the two genetically dissimilar gametes or individual shows increased or decreased vigour over better parent or over the mid-parental value. Earlier, Power (1944)also suggested that the term heterosis should be used only when the hybrid is either superior or inferior to both the parents. Other situation should be regarded as partial or complete dominance. It can be easily explained with the help of Table 5.1.

Introduction to Hi-Tech Horticulture Greenhouse area in different countries

1. Japan - 42,000 (ha)
2. Holland - 10,000 (ha) 3) Italy - 20,000 (ha)
3. USA - 4,000 (ha)
4. India - 700 (ha)
   • Crops are grown in the greenhouse Strawberry, grapes, citrus, banana, papaya, melons.
   • Salt affected soils in different states: UP - 2,375 °
     • Gujarat - 1,334
     • W.B. - 1,150
     • RJ - 458
     • Punjab - 749 Haryana - 636

Hibiscus a well known member of the family Malvaceae, grows as an evergreen shrub plant. The plant is extensively cultivated as an ornamental plant that bears large flowers on the bushy hedges. These enormous flowers are usually dark red in colour and are not usually fragrant. The huge size and the reddish colour and hues attract humming birds (Kumar and Singh, 2012). The generic name is derived from the Greek word “hibiskos” which was the name Pedanius Dioscorides gave to Althaea officinalis. Hibiscus rosa-sinensis was named by Carolus Linnaeus. The Latin term “rosa-sinensis” literally means “rose of China”, though it is not closely related to the true roses. Member species are often noted for their showy flowers and are commonly known simply as hibiscus, or less widely known as rose mallow. The hibiscus is used as an offering to goddess Kali and Lord Ganesha in Hindu worship. Hibiscus is an important flowering and garden plant. It has various medicinal values (Chopra et al., 1996 and Sayed et al., 2012).

ORIGIN AND DISTRIBUTION

The ancestors of the modern exotic hibiscus were originally native to Mauritius, Madagascar, Fiji, Hawaii, China and India. Ornamentally most important species is Hibiscus rosa-sinensis. There is no known record of the history of the domestication of Hibiscus rosa-sinensis. In one view it is believed that the species is a native to China and was first cultivated by the Chinese for its showy flowers (Kimbrough, 1997). No extant wild populations of the species have however, been reported from that country. Another view holds that the species is a native of the South Indian Ocean islands of which Madagascar, the Mascarene Islands and the Rodriguez Islands are a part (Borssum Waalkes, 1966). This view is strengthened by the fact that three Hibiscus species genetically compatible with H. rosa-sinensis are native to this area (Singh and Khoshoo, 1989). It is thought that the species was collected and domesticated on these islands by far easterner during their westward migration.

India is regarded as a horticulture paradise due to growing of tropical, subtropical and temperate fruit crops in varied soil and climatic conditions. However, increasing pressure on land owing to diversion of orchard lands to various other obvious reasons as well as rising energy and land-costs, together with mounting demand for fruits have made HDP imperative to achieve higher productivity from limited space.

There is a shift in farmers’ perception from production to productivity and profitability, which can be achieved through high density planting. India is the second largest producer of fruits in the world. However, the average productivity of various fruit crops in the country is quite less. Low productivity can be attributed to many reasons such as old and senile orchards, lack of canopy management practices, planting of poor yielding varieties, low or no adoption of recommended package of practices by the farmer leading to poor orchard management, etc.

This chapter introduces the evolution of forestry from traditional human dependence on forest resources to a multidisciplinary scientific field concerned with ecological, economic, and social objectives. It traces the development of scientific forestry in Europe and its expansion into India, including the influence of German and French forestry systems. The chapter examines the establishment of forest administration, forest legislation, and the changing role of forestry during the colonial and post-independence periods. It also discusses the evolution of national forest policies, farm forestry, social forestry, participatory forest management, forestry education, and contemporary conservation frameworks. The chapter highlights the transition from production-oriented forestry toward sustainable forest management, biodiversity conservation, community participation, and climate resilience.

WORLD HISTORY

Work on ornamental crops was first initiated in Krelage Nursery founded by Ernst Heinrich Krelage for bulbs, lilium and dahlias in 1811. During 1895, Taiwan Agricultural Research Institute was established for ornamental crops. The first yellow corn Golden Bnatam was listed in Burpee’s Catalogue in 1902. In 1909 Primadonna F₁ hybrid variety of begonia was released by the Benary Seed Company in Germany and in the same year Atlee established seed farms at Floradale in California, Erfurt in Germany Anglia in UK and Kashmir valley in India for annual garden flowers. Luther Burbank did excellent breeding works on ornamental trees, flowers and fruits in 1926.

David developed the first successful hybrid in Nasturtium (1934) and Red and Gold hybrids of marigold (1937). In 1940, Burpee was successful in chromosome doubling and transformation of wild flowers and developed “Bright Scarlet” and “Super Tetra Series” in antirrhinum and “Jumbo Scarlet” in zinnia. He produced new strains of marigold, zinnia, nasturtium and snapdragon. William Houston in 1940 initiated the Ageratum breeding. Morden Research station was set up in Western Canada in 1945 for ornamental crop breeding. In 1968, Dr. Gustav A.L., Mehlquist was recognized as an international authority on breeding of ornamental plants. George J. Ball breeder of peas, asters and calendulas was a pioneer for the supply of dwarf impatiens and orange impatiens ‘Super Elfin’ in 1968. Israeli breeders during 1990 gave more attention towards the development of carnation, lily and anemone varieties. Longwood Gardens in 2008 got success in breeding of stock. The first record of chrysanthemum hybridization, germination and selection of superior seedlings occurred in 1827 by M. Bernet.

Aromatic plants synthesise and preserve a variety of biochemical products, many of which are extractable and useful as chemical feed stocks or as raw materials for various scientific investigations. Many secondary metabolites of plants are commercially important and find use in a number of perfumery, flavouring and pharmaceutical compounds. The characteristic property of the plant is due to a variety of complex chemical compounds and hence aromatic plants are generally referred to as ‘natural bio-chemical factories’ or ‘chemical goldmines’.  Not all these natural chemicals can be synthesised in laboratory. Aromatic plants possess odorous volatile substances, which occur as essential oil, green exudate, balsam and oleoresin in one or more parts, namely, root, wood, stem, foliage, flower and fruit.  The term essential oil is concomitant to fragrance or perfumes because these fragrances are oily in nature and they represent the essence or active constituents of plants. They are called volatile or ethereal oils as they evaporate when exposed to air at ordinary temperatures.