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Plant propagation is one of the important fundamental aspects since the time of Rigveda (1400 B.C.) in the horticulture industry. It refers to the multiplication or perpetuation of individual or group of plants having specific value. Agriculture began, when ancient people started domestication of certain plants by growing around them and now these are cultivated in much improved form due to continuous selection and maintenance of better plant types. During the process of civilization; many plant types were selected for food and other purposes and are classified as food crops, fodder crops, fruit crops, vegetable crops, spices and condiments, aromatic and medicinal plants, etc. Now due modern plant sciences; newer and better plant types came into commercialization for cultivation. However, to maintain their qualities; it needs their propagation to keep their original form. Thus, it indicates that plant propagation has paramount importance in the horticulture industry to supply true-to-type sapling to farmers.

Plant propagation: Plant propagation means multiplication of plant by sexual or asexual method.

There are two type of plant propagation

1.    Sexual propagation

2.    Asexual propagation 

A. Sexual propagation: Sexual propagation means multiplication of the plant by seed. It is also known as seed propagation.

Advantages of sexual propagation

1.    This is the easier and cheaper method.

2.    Some of the plant does not response well by asexual method (Papaya) this type of plant only propagated by sexual propagation.

3.    Sexually raised plant are long lived have extensive root system and bear heavily as compare to asexually propagated plants.

4.    Hybrid can develop only by seed propagation.

5.    Seed can be store for longer duration.

Introduction

Tissue culture refers to the technique of growing cells, tissues, or organs under controlled, artificial conditions. This allows propagation of tissues from a small sample of cells/tissues under sterile conditions. Plant tissue culture is widely used for various purposes, including the production of disease-free plants, rapid multiplication of plants with desirable traits, genetic modification, conservation of rare or endangered species, etc. Tissue culture is a valuable technique because it enables consistent and large-scale production of plants, ensures genetic uniformity, and allows manipulation of plant biology at cellular level. In this chapter, we will discuss about the major types of tissue culture techniques and their advantages, disadvantages, as well as applications in brief.

Different Types of Tissue Culture

Callus culture involves induction and growing of unorganized, undifferentiated cell masses from an explant on a nutrient/culture medium. The part of plant used to induce callus is called explant which can be tissue, organ or part of a plant.

Organ culture is used to maintain or grow an entire organ or a part of organ in vitro. It preserves the structure and function of the organ for studies or transplantation.

Embryo culture involves isolating and growing embryos from seeds or ovules in a nutrient medium. It is used to bypass seed dormancy or rescue embryos from hybrid crosses that may not develop naturally.

Cell suspension culture involves growing dispersed cells or small cell clusters in a liquid medium under continuous shaking. This is used for large-scale production of cells, secondary metabolites, and studying cell biology..

Meristematic Tissues

These are composed of cells that are in state of division or retain the power of dividing. These cells are essentially alike and isodiametric. They may be spherical, oval or polygonal and their walls thin and homogeneous; the protoplasm in them abundant and active with large nuclei, and the vacuoles small or absent.

Classification of Meristems

Meristems are classified in different ways on the basis of certain factors.

A) According to their origin and development

1. Promeristem or primordial meristem

2. Primary meristem and

3. Secondary meristem.

Layout plan- Main objective of layout is maximum number of plant accommodated in per unit area without affecting the efficiency of production. If more than one fruit is included in the orchard plan, each kind should be grouped into individual block. Similarly fruit ripened in particular season should be grouped to facilitate convenience in watch and ward, harvest and post harvest handling. Some kind of fruit trees need pollinators/pollinisers for improving the fruit set.

The system of planting to be adopted is selected after considering the slope of land, planting density, purpose of utilizing the orchard space, convenience, etc. Traditionally, six systems of planting are recommended for fruit trees. However, with the popularity of high density planting, there are some advances inplanting, especially in fruit crops.

1. Square system: This system is considered to be the simplest of all planting systems and adopted widely. In this system, the plot is divided into number of squares as per plant spacing and trees are planted at the four corners of each square, in straight rows running at right angle. Under this system, intercultural operations, spraying, harvesting, etc. can be done conveniently and easily. Cultivation and irrigation can be done in two directions. In this system, plant to plant and row to row distance is the same. e.g. 5x5m, 4x4m, 10x10m, etc.

   While laying out the plot, a base line is first drawn parallel to the road, fence or adjacent orchard at a distance equal to half the spacing to be maintained between the trees. Wooden pegs are fixed on this line at the desired distance. At both the ends of the baseline, right angles are drawn by following the simple carpenter’s 3,4,5 meter system. After the formation of three lines, it is easy to fix all the other pages to mark the tree locations at the required spacing by using ropes connecting the pages of the lines in opposite directions.

   Area occupied per plant = row to row distance X plant to plant distance

Trees are perennial in nature and are valuable elements in a landscape. Trees provide shade, privacy and beauty but a tree in a wrong place may create problem in years to come. To avoid this, care should be taken while selecting trees for landscape. The ultimate size and shape of the mature tree, how much annual maintenance it requires, location and purpose are major considerations while selecting a tree. Matured tree height and tree diameter should fit it into the space when tree is planted. Shape of canopy, colour of flower and season of flowering are also to be considered.

Plants have played an important role in many mythologies and religions of the world and have been known for deep and sacred meanings throughout the ages. It is the symbolic representation of the old cultural traditions in the form of a tree across the world. Christmas tree in the Christianity and Bodhi tree in Buddhism are the example of tree featured in mythology. In most of the folklore, folktales and folk religion trees are often said to be homes of tree sprits. If history is traced, we will found that Druidism and Germanic paganism appear to have involved in religious conservational practices of sacred groves. Druidism is a nature based religion that was taught by Druids about 2000 years ago. The term druid itself possibly derives from the religeous word for oak. Therefore, the Druidism reconnects us with nature, our ancesters and ourselves by working with plants or trees.

Trees are honored in Indian culture for their different beneficial characters like medicinal properties, food, fodder, maintaining hydrological cycle, moderate the climate etc. There are some trees that have attained more than a special benevolence in Hindu mythology. In Indian mythology number of plants have been given sacred value and worshipped by different cultures. Some important plants of sacred value have been discussed here.

• Fast track plum breeding started with an aim to reduce the juvenile period in temperate fruits.
• Transgenic early continuous flowering (ECF) gene induces early flowering.

  Chilling requirement

• Japanese Plum - 700-1000 hrs - below – 7°C
• European plum - 1000-1200 hrs - 7°C
• Clonal rootstock for raising plum plants: Myrobalan B.
• Dwarfing rootstock – P. subcordata
• Planting time – Dec-Jan
• Training System – Open centre system – oldest
• Japanese plums are mostly adaptable to open centre system.
• Fruit thinning agents – DNOC, Ethephon, 3-CPA
• Japanese plum needs thinning upto 25 - 40%.
• European Plum: President, Victoria, Starking Delicious, Greengage.
• Japanese plum – Beauty, Santa Rosa, Mariposa. Kelsey, Frontier, Elephant Heart, Satsuma
• Subtropical – Sultlej Purple, Kala Amritsari, Titron.
• Self-fruitful – Beauty, Mariposa

Poinsettia (Euphorbia pulcherrima) is one of the most popular ornamental pot plants belonging to the family Euphorbiaceae. The botanical name Euphorbia pulcherrima means very beautiful. Euphorbia pulcherrima the name originally given by German botanist Karl Ludwig Wilenow was changed to “Poinsettia” in honour of “Mr. Poinsett” in 1836 by William Prescott. Poinsettia is a short-day, non-food and non-feed vegetatively propagated ornamental plant. Poinsettias are fascinating winter blooming small shrubs or trees which can grow anywhere from about 0.6 to 5 m tall. The coloured bracts of poinsettia plants are actually leaves. Colours of the bracts can be red, pink, orange, white or marbled. These coloured bracts are caused by photo-periodism. Poinsettias have also been called the lobster flower and the flame-leaf flower due to the red colour. California is the top U.S. Poinsettia-producing state.

ORIGIN AND DISTRIBUTION

The poinsettia (Euphorbia pulcherrima) is the world’s most economically important potted plant but despite its preeminence it is not clear which wild populations are ancestral to the varieties cultivated around the world. Tradition holds that the U.S. envoy to Mexico, J. R. Poinsett collected the progenitors of the over 300 varieties in global cultivation on an 1828 excursion to northern Guerrero State, Mexico. It is unknown whether the contemporary cultivars are descended from plants from Guerrero or whether germplasm from other parts of poinsettia’s 2000 km long distribution entered into cultivation during the nearly 200 years of subsequent poinsettia horticulture.

The combination of nuclear and plastid haplotypes characterizing cultivars was found only in northern Guerrero. Whereas, distribution modeling indicated that suitable habitat conditions for wild poinsettias are present in this area consistent with their likely wild status. Finding shows that the area of northern Guerrero as the cultivated poinsettia’s probable ancestral region congruent with the traditional account attributing the original collections to Poinsett (Trejo et al., 2012). Poinsettias are originally native to the central and southern parts of Mexico where they grow as a shrub up to 5 m. The poinsettia was introduced to the United States by Mr. Poinsett in 1828. It was introduced into North America in1838 from Mexico and since that time it has achieved considerable economic importance as a pot plant in the florist trade.

Pollination

Pollination is the act of transfer of pollen from the male reproductive organ of a plant to the female reproductive organ of same or another plant.

Pollination is of two types

5.1 Self Pollination

It is the transfer of pollen from the anther to the stigma of the same flower from where the anthers have been generated. Thus, self pollination happens to be denominated as autogamy where auto means “same” and gamy means “union”. Obviously, self pollination is found in bisexual flowers. But self pollination also occurs when pollen is transferred from one bisexual flower to the stigma of another bisexual flower of the same plant. This kind of autogamy is known as geitonogamy. So, self pollination has to be defined keeping the parent plant on the main focus not the flower. If genetical consequences are considered, self pollination promotes the occurance of purelines or homozygotes. Self pollinating species developed themselves evolutionary fit due to homozygous balance and do not exhibit significant inbreeding depression.

The emasculation and pollination in any crop depend on its floral structure and biology. Cashew is basically andromonoecious (polygamo monoecious) crop with staminate and hermaphrodite flowers appearing in the same inflorescence or panicle (Rao and Hassan 1957). The hermaphrodite/bisexual/perfect and male flowers occur intermediately in the same panicle in three or four phases viz., Male – Mixed – Male or Mixed – Male – Mixed– Male. Distinct phases depend on genotype and environmental factors. The tree shows considerable variation in the duration of different phases. The hermaphrodite flowers are bigger in size than staminate or male flowers (Damodaran et.al., 1965). Male flower always exceed the bisexual flowers. The flowers produced early in a panicle are mostly male (Rao and Hassan 1957; Damodaran et. al., 1965). Most of the perfect flowers are produced about one month after the first flower occurred in the panicle (Bhat et al., 1998).

Pollination : Transfer of pollen grains from anther to stigma is termed as pollination.

1. Self pollination

When pollen grains from an anther fallen to stigma of same flower or same plant is called self pollination or autogamy. In other word when pollen and pistil are from the same plant, often (but not always) from the same flower. In other word when transfer of pollen grain from anther to stigma of a same flower or same plant is called as self pollination. In which development of seed by self pollination is known as autogamy it is the closest form of breeding. Autogamy leads to homozygosity such species develop homozygous balance and do not exhibit significant inbreeding depression several mechanisms which promote autogamy.

• 52% use of the fruit of total weight.
• Comprising 78% juice and 22% seeds
• CIAH – Central Institutes for Arid Horticulture, Bikaner.
• Cultivars – Alandi or Vadki, Bassein Seedless, Dholka Ganesh, Jalore Seedless, Jyothi, Kabul, Kandhari, Paper Shell, Muskat Red, G-132, G-107, G-137, P-13, P-16, P-23, P-26.
• Hybrid – Mridul – Ganesh × Gul-E-Shah Red (fruit spot infection)
• Ruby – M
• Type of inflorescence is Hypanthodium.
• Pomegranate is a subtropical fruit crop.
• It is commercially propagated by hardwood cutting.
• Fruit cracking happens due to baron deficiency and water imbalance.
• HDP - (5 m × 2 m)- 1000/ha

  (5 m × 5 m)- 400/ha

Pomegranate (Punica granatum L.) commonly known as ‘Anar’, is an ancient favourite table fruit of tropical and subtropical regions of the world. It belongs to family, Punicaceae. There is a great diversity in pomegranate. Originated in southern Asia and said to be native from Iran to the Himalayas in the northern India (Sandhu and Gill, 2013). The orcharding of pomegranate is somewhat expansive and skilful job. The perennial nature of fruit take longer period to come to bearing stage (3-4 years). Thereafter due to neglect in management practices the annual production, many a times compels the orchardist have to go for distress sale of entire poor quality produce. But, the successful commercial fruit production with increased quality production has made significant contribution to economic development of the grower and ultimately those in the growing region in sustainable manner.

INTRODUCTION

Total forest cover in India is 70.17 million ha or 21.34% of the total geographical area according to the State of Forest Report 2015. Of this, 8.59 million ha (2.61%) is very dense forest, 31.54 million ha (9.59%) is moderately dense and rest of 30.04 million ha (9.14%) is open. The trees outside forest (TOF) are estimated to cover 9.26 million ha area which constitutes about 2.82% of the total geographical area of the country.  Thus the total forest and tree cover of the country is 24.16% of the total geographical area. The estimates of total growing stock of forest and TOF are 4.195 billion m3 and 1.573 billion m3, respectively. The total growing stock of wood in the county is estimated to be at 5.678 billion m3 which implies an average growing stock of 72.63 m3 ha-1 in 79.42 million ha of forest and tree cover (FSI 2016).

World trade of fresh cut flower is increasing day by day. Fresh flowers lose their freshness and quality both during travel and also during and after arrangements due to flower specific short vase life. Such deficiencies can be ameliorated through application of nutrient additives to vase water. Use of preservative solutions has been known for many years to increase the vase life of cut flowers. Different methods like use of holding, pulsing and bud opening solution, growth regulators, gamma irradiation, pre-cooling, cold storage, packaging etc. are already in practice in the flower trade to ensure the garden fresh quality of flowers to consumers (Singh et al., 2001). The floral business is expanding in a big way, nationwide.  Sales of cut flowers are rising faster through retailers as every state has entered into development of supermarket chains. Cut flower sale through retailers is  a booming business. Retail growers and Wholesale growers are the major players in our country for domestic market. Retail market suffers from logistic bottlenecks. Retailers do not have any background scientific knowledge to increase the vase life of cut flowers. Flower buyers are very choosy and prefer quality bloom with longer vase life.

Introduction

Once the cashew fruits fallen, are collected, the nuts are to be separated from the cashew apple and sun dried for 2 to 3 days. This is very important in view of the quality of kernels obtained during subsequent processing. Raw nuts at the time of harvest will have moisture content ranging between 16 to 25 per cent; while sun dried nuts have moisture content of 8 to 10 per cent (Nagaraja, 2000). If the nuts are not dried and stored properly, it leads to fungal spoilage resulting in poor quality of kernels. Fungi such as Gonatobotryum, Helminthosporium sp., Corynespora sp., Alteraaria sp., Verticillium sp. and many species of Aspergillus have been isolated from stored cashew nuts (Joseph, 1981). In view of the occurrence of fungi, drying of the raw nuts before storage becomes absolutely essential. Maximum permissible moisture content of raw cashew nuts is 8.7 to 9.1% (Ohler, 1979). Okwelogu and Mackay (1969) have reported that high whole nut or kernel moisture percentages are closely associated with high shell moisture contents and for nuts whose moisture content is in equilibrium with the ambient relative humidity, the moisture content of whole nut and kernel could be predicted from that of shell with more than 99% accuracy. A higher kernel shell weight ratio has been shown to be inversely proportional to moisture content.

Importance of Post Harvest Technology

1.     Proper handling, packaging, transportation and storage reduce the post harvest losses of fruit and vegetables. For every one percent reduction in loss will save 5 million tons of fruit and vegetable per year.

2.     Processing and preservation technology helps to save excess fruit and vegetable during the glut season (off season).

3.     The technology has become a necessity to improve the food safety and strengthen nation’s food security.

4.     The technology helps to boost export of agricultural commodities in the form of preserved and value added products.

5.     Presently mango, pineapple, citrus, grapes, tomatoes, peas, potato and cucumber being processed on a large scale.

Post-harvest deterioration primarily means the loss of commodity after harvest. It is one of the serious problems for producers, distributors and consumers. Post-harvest deterioration of perishables is the spoilage of fruits and vegetables after harvesting. Perishables are the feed commodities with moisture content between 50 and 90%. Fruits with the size of 5 g to 5 kg or more, high to very high rate of respiration, high generation of heat, soft texture, easily damageable nature and shorter shelf life are more prone to the microbial damage. The post-harvest losses are caused either by pathological, physiological and mechanical means or combination of all these. These perishables because of having minerals, vitamins, and photochemicals (essentially required for the human health) become victim to the several biotic and abiotic factors easily. Once these fruits are detached from the plant are deprived of essential ingredients as hormones, nutrients, water and other requisites. These biotic and abiotic factors apart from rendering the produce unfit for human consumption reduce the aesthetic value, substantial decrease in food value and also organoleptic quality of the vegetables. The fleshy produce are prone to the exogenous agencies such as fungi, bacteria, etc. as well as other endogenous factors such as respiration, senescence, etc. which may lead to poor quality of the produce and also affect market quality. These perishables continue to respire and may carry out various biochemical activities, leading to ripening subsequently to senescence and making them more susceptible to microbial damage. In addition, tropical conditions prevailing in India, which are characterized by high temperature conditions, are optimal for fungal and bacterial infections. In India, the post-harvest losses of fresh fruits and vegetables are estimated to be about 30%. The post-harvest losses have been recorded are 5-20% at farmer’s field due to pre-harvest infection and sorting, 15-20% due to faulty packaging, 30-40% during transportation and 30-40% during marketing.