eChapters

A. Case study: Breeding for disease resistance and yield improvement in Black pepper (Piper nigrum)
Black pepper (Piper nigrum) is one of the most important spice crops globally, often referred to as the “King of Spices.” However, its production is frequently threatened by various diseases, particularly Phytophthora foot rot, which can cause significant yield losses. Over the years, extensive breeding efforts have focused on developing disease-resistant and high-yielding varieties of black pepper.
1. Background
• Primary disease concern: Phytophthora foot rot (caused by Phytophthora capsici) is a major disease affecting black pepper, leading to root and stem rot, defoliation, and ultimately, plant death. Other diseases include slow
decline (caused by nematodes and fungal pathogens) and viral infections.
• Yield challenges: The average yield of black pepper is relatively low, primarily due to the susceptibility of many traditional varieties to diseases and pests, along with poor management practices and climatic challenges.
2. Objectives of breeding programs
• Disease resistance: To develop varieties resistant to Phytophthora foot rot and other significant diseases.
• Yield improvement: To increase the overall productivity of black pepper plants, focusing on higher berry yield, larger berries, and better quality.
• Quality enhancement: To maintain or improve the quality of black pepper in terms of essential oil content, piperine content, and other flavour components.
 

A) Conventional breeding techniques
Conventional breeding techniques for spices involve various methods to improve traits such as yield, quality, disease resistance, and adaptability. Here’s an overview of conventional breeding techniques applied to specific spices:
1. Black pepper (Piper nigrum)
• Selection: Choose superior plants based on traits like yield, quality of peppercorns, and disease resistance.
• Hybridization: Cross-breed different varieties to combine desirable traits.
• Mutation breeding: Induce mutations to develop new traits or enhance existing ones.
2. Small cardamom (Elettaria cardamomum)
• Selection: Select plants with high yield, better quality, and disease resistance.
• Hybridization: Cross different varieties to improve traits such as pod size and essential oil content.
• Seed production: Improve seed production and quality through selection.
3. Large cardamom (Amomum subulatum)
• Selection: Identify plants with higher yield and better resistance to pests and diseases.
• Hybridization: Develop hybrids to enhance flavour and size of the cardamom pods.

Germplasm collection and conservation are crucial for maintaining the genetic diversity and ensuring the long-term sustainability of spice crops. Here’s a general approach for spices:
A. Germplasm collection
a. Identification and selection
• Identify key spices and their varieties.
• Select diverse genetic material, including wild relatives and landraces.
b. Field collection
• Collect samples from diverse locations to capture genetic variation.
• Document the collection site, local environment, and plant characteristics.
c. Data recording
• Maintain detailed records on the collected germplasm, including geographic origin, agronomic traits, and any special characteristics.
d. Quality assessment
• Ensure the collected material is healthy and viable.
• Assess quality traits relevant to the spice industry.

A. Principles of spices breeding
Plant breeding is a science-based technique aimed at improving the genetic makeup of plants to develop desirable traits such as increased yield, resistance to diseases and pests, improved quality, and adaptability to various environmental conditions. When applied to spices, these principles are adapted to meet the unique characteristics and challenges of spice crops.
A.1. Understanding the breeding objectives
• Yield improvement: Increasing the yield of spices like black pepper, ginger, and turmeric is a primary goal. This involves selecting varieties that produce more berries, rhizomes, or seeds.
• Quality enhancement: For spices like vanilla, cinnamon, and saffron, the focus is on enhancing the quality of the product, such as increasing vanillin content, oil content, or curcumin levels.
• Disease and pest resistance: Developing resistant varieties is crucial for spices prone to specific diseases, like black pepper’s vulnerability to foot rot or large cardamom’s susceptibility to fungal diseases.
• Adaptation to environmental conditions: Breeding efforts often aim to produce varieties that can thrive in specific climates, such as the cool, shaded forests required for large cardamom or the arid conditions suitable for cumin.

Spices breeding is the process of developing new and improved varieties of spices through selective breeding, hybridization, and other genetic techniques. The main goals of spices breeding are to enhance desirable traits such as yield, flavour, aroma, disease resistance, and adaptability to different growing conditions. Importance of spice breeding
• Economic value: Spices are economically valuable crops globally. Breeding can lead to higher yields and better quality, improving profitability for farmers.
• Diversity and culinary use: Spices like black pepper, cardamom, turmeric, and chili are used worldwide in various cuisines. Breeding helps to maintain and improve the diversity of these spices.
• Medicinal properties: Many spices have medicinal properties. Breeding can enhance the concentration of beneficial compounds in spices.
Breeding objectives
• Yield improvement: Increasing the quantity of spices produced per plant or per unit area.
• Quality enhancement: Improving the essential oil content, pungency, colour, and other quality traits.
• Disease and pest resistance: Developing varieties that are resistant to common diseases and pests affecting spices.
• Climate adaptation: Creating varieties that can withstand different climatic conditions, such as drought or high humidity.
• Reduced crop duration: Breeding for shorter growth cycles to increase the number of harvests per year.

Location

Bundelkhand is a geographical and cultural region and also a mountain range in central & North India. The hilly region is now divided between the states of Uttar Pradesh and Madhya Pradesh with the larger portion lying in the latter state. Bundelkhand region is situated between Vindhya range in south and Indo-Gangetic plain in north and lies between 23º10’ and 26º27’ N Latitude and 78º4’ and 81º34’ E Longitude, covering an area of 70,747 sq km. It spread over two states viz. Uttar Pradesh and Madhya Pradesh and comprises of 14 districts. It is spreading over of seven districts of Uttar Pradesh viz. Jhansi, Lalitpur, Jalaun, Hamirpur, Mahoba, Banda and Chitrakoot and similarly seven districts of Madhya Pradesh viz. Sagar, Panna, Tikamgarh, Chhatarpur, Damoh, Datia and recently formulated Niwari district (Fig. 1). The language spoken in Bundelkhand is Bundelkhandi and Hindi. The total population of Bundelkhand as 2011 census is 1,83,35,044.

1. The term extension is __________ word. (Latin)
2. _____________ is the father of extension. (Leagnes)
3. The term extension was first used in _____________. (U.K.)
4. The goal of the extension is to promote ______________. (Scientific out-look)
5. The success of rural projects depends upon ______________. (Agricultural Extension)
6. The National Extension Service was started in the year ___________. (2nd Oct., 1953)
7. Training of Rural Youth for self employment was started in the year _____________. (1980)
8. Agriculture extension was first started in ________________. (Bihar Agricultural University, Sabour)
9. T & V programme was introduced in ____________. (1974)

1. _____________ coined the term wildlife. (William Hornday)
2. National Biodiversity Board is situated at_____________. (New Delhi)
3. The term biodiversity was coined by_____________. (Edward Wilson)
4. The study of combination of animal behaviour and ecology is called as_____________. (Ethology)
5. _____________ is the place where an organism or community lives. (Habitat)
6. _____________ is the living medium of the habitat in a wetland. (Water)
7. _____________can be defined as the functional status of an organism in an ecosystem or the role played by a species in its natural habitat. (Niche)
8. The food which is totally non-nutritive and is consumed by the animals only to fulfil its stomach is called ___________. (Stuffing food)
9. The period in which the food is not sufficiently available and causes trouble to the animal, is called __________. (Pinch period)

1. A measure of the items in a random sample is known as_____________. (Statistics)
2. Most commonly used measure of central tendency is_____________. (Arithmetic Mean)
3. Mean deviation is the_____________, when calculated about median. (Least)
4. Arithmetic mean of central tendency is applied for calculation of_____________ and _____________. (Regression and Correlation coefficient)
5. Arithmetic mean and variance are always equal in_____________ distribution. (Poisson)
6. Geometric mean of central tendency is appropriate for_____________. (Index number)
7. _____________ is the sum of all values/ number of values. (Arithmetic mean)
8. Middle most item of all values is_____________. (Median)
9. _____________ measure of central tendency requires data arrangement in ascending or descending order for its estimation. (Median)

1. An imaginary line joining the points of equal elevation on the surface of the earth represents_____________. (Contour lines)
2. The vertical distance between consecutive contours lines is called_____________. (Contour interval)
3. When measured with a compass, the line of sight that run midway between due North and due West has a bearing of_____________. (N 45 degree W)
4. A survey of a large area which consider the earth’s curvature is called a _____________ survey. (Geodetic)
5. The distinctive feature of topographic maps is the_____________ of the land. (Shape or Contour)
6. The colour used to depict fence lines and important roads on a topographic map is _____________ and_____________. (Red and black)
7. A surveyor’s chain is divided into links and each link is_____________ long. (0.66 feet)
8. A body of water on a topographic map is presented by_____________ colour. (Blue)
9. On a topographic map a ploughed field would be sown as_____________ colour. (White)

1. The technique of acquiring information about some object by recording devices, without being in physical contact with the object under study is_____________. (Remote sensing)
2. The term remote sensing was coined by ____________. (Ms. Evelyn Pruitt)
3. Father of Indian remote sensing is ________________. (Pisharoth Rama Pisharoty)
4. In remote sensing_____________ type of radiation is utilized for photography.(Infra red)
5. First aerial photograph was taken in 1858 by Felix Tournachon, known as ________, from a tethered ballon over the Bievre Valley in France. (Nadar)
6. Remote sensing systems which provide their own source of energy for illumination are known as_____________. (Active sensors)
7. _____________ sensors are very useful in detecting and mapping forest fires. (Thermal sensors)
8. Land cover classification using three levels of the scale of 1 : 100000 was developed by _____________agency. (NRSA)
9. The forest survey of India (FSI) conducts forest cover survey once in every_____________. (2 years)

1. ‘Agrostoogical’ refers to cultivation of_____________. (Grasses)
2. Land on which the potential plant community is composed periodically of native grass, forbs and shrubs valuable for forage and in sufficient quantity to justifying grazing use is called as_____________. (Rangeland)
3. The basic object of range management is to_____________ and_____________ the rangeland for higher production of fodder, rich in nutritive value and palatability. (Improve & maintain)
4. ICAR had carried a grassland survey of India in 1954 by Wnyte, Venkatramanan and Dabadghao had found_____________ types of grass land in different environment. (8)
5. The main reason for the enormous increase in livestock population is_____________. (Social attitude of the people)
6. All the vegetation consumed by grazing animals including fruits and twigs of trees and shrubs is called_____________. (Pasturage & forage)
7. The practice by which the gap can be bridged between the requirement and availability of animal feeds and fodder is known as_____________. (Grass land techniques)
8. The scientists_____________ have recognised five type of grass covers in India. (Dabadghao & Shankar Narayan, 1979)
9. The maximum area of about 2.8 milloin square kilometre is covered under_____________ type of grassland in India. (Phragmites Saccharum type)

1. A plant cell constitutes the_____________ per cent of water. (80-90%)
2. Photosynthesis is the process of conversion of_____________ energy into _____________ energy. (Radiant, Chemical)
3. Dark reaction of photosynthesis occurs in_____________ and light reaction occurs in _____________ region of chloroplast. (Matrix, Lamellae)
4. The connecting link between glycolysis and Kreb’s cycle is_____________. (Pyruvic acid)
5. _____________ is responsible for turgidity of plant cells. (Osmosis)
6. A diffusion of liquids through a semi-permeable membrane is known as_____________. (Osmosis)
7. The diffusion of solvent particles into a living cell is known as_____________. (Endo-osmosis)
8. The diffusion of solvent particles out of a living cell is known as_____________. (Exo-osmosis)
9. The outward pressure exerted by the cell solution on the cell wall which is developed due to osmotic diffusion of water is called as_____________. (Turgor pressure)

1. Forest soils are_____________ in nature. (Acidic)
2. The term soil originated from latin word ‘Solum’ which means_____________. (Floor)
3. Most commonly used sources of N fertilizer in forestry is_____________. (NH₄)₂SO₄
4. _____________ is considered as the father of pedology. (V. V. Dokuchaev)
5. _____________ is considered as father of soil testing. (Troug)
6. Land capability class was given by_____________ and_____________. (Klingebiel and Montgomery)
7. The law of the minimum was given by_____________ in 1862. (Liebig)
8. Law of restriction was proposed by_____________. (Liebig)
9. The soil structural classification followed in India is according to_____________. (USDA)

1. _____________ is the main source of energy for the earth. (Sun)
2. The major cause of ‘Minamata’ disease in Japan was due to pollution by_____________. (Mercury)
3. _____________ method in water analysis is used for the quantitative estimation of dissolved oxygen. (Modified Winkler’s method)
4. Systematic scientific study of fresh water ecosystem is called as_____________. (Limnology)
5. The Earth Summit held in 1992, was organised at_____________. (Rio de Janeiro)
6. The altitude of troposphere in atmosphere is about_____________ kilometre. (Upto 11 km)
7. Bhopal gas tragedy took place in the year_____________. (1984)
8. The unit of noise pollution is measured in_____________. (Decibel)
9. Most important mitigation strategy for reducing global warming and climate change is _____________. (Carbon sequestration)

1. _____________ is a fertilize ovule consisting of intact embryo stored food and seed coat, which is viable and has got the capacity to germinate. (Seed)
2. The content of other than seeds such as straw, soil particles, small stones and seeds of other species is called_____________. (Inert material)
3. Initial seed obtained from the selected individual plants of a particular variety or parental line, used to produce breeder seed and forms the basis of the total seed production chain is_____________ seed. (Nucleus)
4. The loss of weight of a sample when it is dried due to decrease in_____________. (Moisture)
5. A specified quantity of seed, physically identifiable in respect of which an international analysis certificate may be issued is called_____________. (Seed lot)
6. The blowers are used for_____________. (Purity of seeds)
7. A type of seed that is not certified but the labelling is done to indicate the quality or standard in a truthful manner is called_____________ seed. (Truthfully labelled seed)
8. The reagent used for biochemical test for determining seed viability is_____________. (Tetrazolium chloride or Bromide)
9. The food material contained in_____________that nourishes the embryo during its development. (Endosperm)

1. Term biotechnology was coined by_____________. (Karl Ereky)
2. Applied use of molecular biology and recombinant DNA technology is _______. (Biotechnology)
3. Bacteria are present everywhere except in_____________. (Distil water)
4. The branch of science dealing with the study of microbes is known as _______. (Microbiology)
5. The unique properties of each amino acid are determined by its particular_____________. (R-groups)
6. Optical isomerism arises from the presence of a/ an_____________ atom. (Asymmetric carbon)
7. In the breakdown of glucose to pyruvic acid,_____________ enzyme catalyses the successive steps. (Eleven)
8. _____________ plants are used to accumulate pollutants from soil thus clears soil from pollutants. (Phytoremediation)
9. _____________ contains only one type of nucleic acid. (Virus)

1. Tree improvement consists of_____________ and_____________. (Silviculture and tree breading)
2. Variation within the local progenies is due to_____________. (Environment)
3. Larger units of similar vegetation are referred as_____________. (Biome)
4. _____________ is the only gaseous hormone. (Ethylene)
5. More genetic gain can be achieved in the_____________ seed orchard. (Clonal)
6. _____________ is known as father of genetics. (Mendel)
7. Pure line theory was proposed by_____________. (Johannsen)
8. Self-incompatibility hampers the development of_____________. (Inbreds)
9. Progeny having both parent identities is known as_____________. (Fullsib)

1. Life history of Indian termite was first published by_____________. (J.G. Koeing)
2. Plant disease written by_____________. (R.S. Singh)
3. J. W. Helfer seems to have the first to collect insects because these were pest of trees during the year_____________. (1810)
4. The largest insect order is_____________. (Coleoptera)
5. Project directorate of biological control is located at_____________. (Bangalore)
6. Main skeletal structure to which most of the muscle attached is_____________. (Thorax)
7. The outmost waxy layer of insect body is called_____________. (Cuticle)
8. Insect body consists of series of segment designated as_____________. (Sclerite)
9. _____________ is the author of the book entitled “The ecology of forest insect”. (CFC Beesom)