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Vegetative propagation enables horticulturists to multiply plants while retaining desirable genetic characteristics of selected mother plants. This chapter covers important methods including cuttings, layering, budding, grafting, division, and specialized vegetative structures. The physiological basis of rooting, wound healing, callus formation, compatibility, and environmental requirements is discussed. Grafting and budding are particularly important for combining desirable scion characteristics with suitable rootstock traits such as vigor, disease resistance, stress tolerance, and adaptation. Proper sanitation and selection of healthy propagation material are emphasized to minimize pathogen transmission. Modern techniques such as rooting hormones, mist chambers, protected propagation, and clonal multiplication further improve the efficiency and uniformity of vegetative propagation.

The inorganic residue remaining after the destruction/burning of organic matter in foodstuffs is called ash. Ash contents in food commodities roughly represent the mineral contents, however, some minerals are likely to be lost due to volatilization or some interaction between constituents during ashing. Estimation of ash content is also used as a measure to judge the quality of fruit products. The presence of high ash content or low alkalinity of the suggests the presence of adulterants in the sample. Similarly, the presence of a high amount of acid-insoluble ash indicates the presence of sand and other silicious matter in the food products.
Principle
Organic matter is burnt off at as low a temperature as possible. Heating is done in stages, first to char the product thoroughly and finally to ash it at 550ºC in a muffle furnace. The inorganic material left after burning organic matter is cooled and weighed.
Apparatus
* Silica Crucibles
* Muffle furnace
* Desiccator
* Balance

ABSTRACT

Grape is a commercially cultivated fruit crop in India. As a result of large-scale introduction, the origin and authenticity of many grape varieties is unclear and the subject of some controversy. Many of the varieties were mixed up and now have led to confusion regarding their correct identification. To explore the genetic relationships between different grape varieties and to analyze their diversity, molecular marker technique has proved to be useful. In the present study, 34 grape varieties have been characterized using Inter Simple Sequence Repeat (ISSR) markers. Out of 93 ISSR primers screened initially, 11-showed good polymorphism. Total 174 bands were obtained, out of which 145 were polymorphic. The similarity indices were calculated using DICE coefficient and NTSYS Pc 2.1 software.

Cluster analysis resulted in the formation of two main clusters. One, of the varieties belonging to Vitis vinifera and other of V. labrusca. Varieties belonging to V. vinifera appeared more diverse by forming distinct sub-clusters based on their colour, flavour and seeds. Out of 34 varieties screened, 10 varieties are grouped in white berries cluster and 15 in coloured (red /black) berries cluster. Three white varieties- Italia, Queen of Vineyard and Thompson seedless are grouped into cluster of coloured varieties. The cluster of labrusca varieties showed homogeneity and had five varieties except Dakh, which belongs to vinifera. It may be wrong introduction. Concord separates initially from all other varieties. Incidentally, Concord is a pure selection from V. labrusca, while varieties like Bangalore blue, Black Muscat, Catawba and Muzzafar Nagar in labrusca group may be the hybrids of labrusca x vinifera. The current study thus revealed that genetic relationship among grape cultivars could be assessed using ISSR markers.

ABSTRACT

DNA based molecular marker technologies have been used extensively to study the genetic relationships and to develop markers for identification of cultivars/varieties in various crops. The present study is an attempt to assess the variability and relatedness among 49 varieties/ accessions of black pepper (Piper nigrum L) based on the data generated by both RAPD and AFLP analyses. The similarity matrix was subjected to cluster analysis and dendrogram generated using the software Ntsyspc 2.1. The dendrogram revealed an average similarity of 63 per cent among accessions. Two selections from the variety Karimunda, namely Sreekara and Subhakara, together formed a single cluster with almost 92 per cent similarity. The dissimilarity observed between the varieties Panniyur 1 and Panniyur 3 the progenies of the same parentage, Uthirankotta and Cheriyakanyakadan was only 18 per cent. Based on RAPD analysis 34 varieties were grouped into 5 clusters. Distinct clustering was not observed in AFLP analysis. The results obtained were correlated with the taxonomical interrelationships reported for these varieties. The overall study has thus helped in understanding the relationship among the local cultivars and released varieties.

Availability of genetic variability and exploitation of this variability are two basic requirements of successful crop breeding programmes. The presence of genetic variation in a character is a must for any improvement in that character. If all the individuals in a population are identical i.e. no variation is there, selection cannot be done, so no improvement will be done. In case of crops, if variation is not present or variation is very small, variations are created through hybridization, mutation, polyploidy etc. Selection is identification and isolation of desirable plants from a variable population. A population consists of large number of heterogenous individuals with different morphological expressions. This variability is contributed by three factors: 1. Genetic constitution of individual, 2. Environmental effect on the individual and 3. Interaction effect between gene and environment. These three factors contribute towards the phenotypic variability among the individuals of the population. Assessment of magnitude of variability present in a crop species is of utmost importance as it provides the evidence for effective selection. Phenotypic variation in a population arises due to genotypic and environmental effects.

Withania somnifera, also known as Ashwagandha, Indian ginseng, Winter cherry, Ajagandha, Kan-aje Hindi, Amukkara in Tamil and Samm Al Ferakh, is a plant in the Solanaceae or nightshade family.

Grasshoppers : Trilophida an- nulata : Acrididae

The nymphs and adults of this grasshopper feed on the margins of leaves of Aswagandha. It is only a minor pest, nibbling on the leaves.

Atuna racemosa subspecies racemosa is shown to be a species with many uses throughout its range from Malaysia to Melanesia. The principle uses described are the seed oil used as anti-inflamatory massage oil and for hair dressing, the seed pulp that is used for caulking and varnishing boats, the wood for house construction, and many parts of the plant as ingredients of local medicines. The medicinal uses of this species deserve further study since it has been shown to have both anti-bacterial and anti-inflamatory action. It is also uncertain whether its distribution over the Pacific is natural or human caused.

Atuna racemosa Rafin. is a widely distributed tree species that ranges from the Malay Peninsula through Malesia, Australasia and to the Pacific islands as far as Fiji, Tonga and Samoa. As with many useful plants, its natural distribution is uncertain because it has been transported around from island to island during the various migrations of the Pacific islanders.

Augmentation strategies are generally used as a way to improve the effectiveness of passive or active frost protection methods. As the intensity of the frost is quite variable and sporadic in nature, simple application of the protection methods is not always helpful in giving adequate protection against freezing stress. 

Nutrition affects the development of human body immune system, playing a vital role in modifying the threat of exposure and susceptibility to infectious patho gens, acuteness of morbidity and success of treatment. Keeping in view the pres ent pandemic and emerging viruses such Eastern Equine Encephalitis, Hantavi ruses, Nipah viruses for which no effective preventive and curative treatment is in hand, a healthy immune system could be one of the most important defences to f ight off (El-Gamal et al., 2011). Vegetables are rich source of essential vitamins, minerals, antioxidants and dietary fibres; however, certain vegetables hold more significance in view of boosting immunity.

Protected structures such as cloches, low tunnels, greenhouses, livestock housing, aquaculture facilities and phytotrones provide varying degrees of climate control for agricultural production. While cloches and low tunnels provide only passive modification of the growing environment, all other protected structures have the possibility of active and precise growing environment control.

GREENHOUSE TECHNOLOGY

Greenhouse technology permits the management of both biotic and abiotic stresses related to plant growth to maximize the productivity and quality. Resultantly, profitability per unit area per unit time increases by two to three orders of magnitude as compared to open field cultivation. Introduction of greenhouse technology in India for commercial production is only about three decades old, whereas it is more than two centuries old in USA, UK, Holland, France, Italy, etc.

A greenhouse is a framed or inflated structure covered with a transparent or translucent material where plant environment could be, at least partially controlled and which is large enough to permit a person to enter and carry out cultural operations. A greenhouse is called a glasshouse, when covered with glass, and plastic house when plastic is the glazing material. Materials of construction and cropping activities are also used to identify greenhouses. In addition to the growing facility, a commercial greenhouse complex has a head house, an input storage and a sales area. All these components are so arranged that unwanted movements of man and material are obviated.The greenhouse complex should have adequate wind breaks on the upstream side of the wind direction to provide protection against heavy winds.

The avocado (Persea americana Mill) belonging to the family Lauraceae, may have originated in southern Mexico but was cultivated from the Rio Grande to central Peru long before the arrival of Europeans. It was then distributed into the West Indies and all other parts of the tropical and subtropical world. It reached India probably in 1892. Now it is grown in Tamil Nadu, Karnataka, and Kerala in India, to a limited extent.

Cultivars/Varieties: Fuerte, Hass, Lula, Pollck, Purple, Green.

Uses

Avocado is low in sugar content and rich in avocado butter, hence can be highly recommended as a high energy food for diabetics. The pulp of the fruit can be preserved by freezing, and can be used as sandwich filling or spread, in ice creams and milk shakes. Though the fruit is high in fat content, it is harmless unlike animal fat. The pulp has butter consistency, and looks very much like cow’s butter and bland in taste with a nutty flavour, without any offensive odour or smell. Its aroma resembles that of tender coconut.

Avocado is a pear-shaped berry, which is otherwise known as alligator pear or butter fruit. The term alligator pear is due to the scaly appearance of some varieties of avocado, especially, the Hass avocados, which possesses black leathery skin. The edible portion of avocado is its flesh, which is yellowish green in colour. This flesh has a slightly nutty flavour with the consistency of butter. Avocado contains vitamins A, C, and B complex that sustain accurate performance of neurotransmitter which makes it as a superior food for the nervous system. Avocados have the highest energy value (245 cal/100 g) of any fruit besides being a reservoir of several vitamins and minerals (calcium and magnesium) that can calm and aid restorative sleep which is essential for nervous system to perform efficiently. Avocado is mainly used fresh, in sandwich filling or in salads. It can also be used in ice creams and milk shakes and the pulp may be preserved by freezing. The end quality of avocado determines its nutritional value. 

Genus : Persea 
Species : americana Mill.
Family : Lauraceae
Chromosome No. : 2n =24

Horticulturally avocado (Persea americana) is a new fruit and is often considered to be  one of the most   nutritive of present day fruit crops. Genus Persea, name has been derived from ancient Greek name of an Egyptian tree with sweet fruits  derived probably from Perseus. Avocado belongs to the family Lauraceae, has 47 genera and about 1900 species. Genus Persea has some 150 species of tropical evergreen trees. Its discovery in the new world has contributed much to the human diet. Fruit is a rich source of fat (27%), 2 per cent protein content is higher than in any other fresh fruit, mineral like Ca, Cl, Fe, Mg, P, K, Na and sulphur are also higher that makes avocado as a useful food which produces more of base forming elements rather than acid forming elements that are found more only in nut crops.

• Avocado is a dense and evergreen tree can grow about 80 feet height
  • Single seeded berry - Fat – 24-26%
  • Recalibrate seeds - Sugar – 1% Rich in K, Fe, Vitamins – B
• Its energy value is twice as much as Banana fruit.
• Most leading avocado cultivar in the world – Fuertes.
• Avocado is classified into 3 distinct horticultural races.
  1. Mexican – Coli fried Duke Pernod
  2. West Indian – Pollock, Simmonds, Black Prince Fuchsia, Peterson, Waldin.
  3. Guatemalan – Taylor, Linda, Queen, Benit
• Duke seedlings are resistant to root rot and cold hardiness.
• Important to salad fruit

The problem of frost damage to the crops is as old as agriculture is. Earlier, crop production was undertaken only as per the geographical distribution of the plant species and the cultivation of crops in non-conventional areas was not there. Later, with the expansion of agricultural activities, a number of introductions were made in the non-conventional areas. 

Euphorbiaceae is a large family that includes 310-320 genera and 7500 species (Walters and Keil, 1996). Two centers of distribution for this family are tropical America and tropical Africa. The family has an extremely variable life span and habits are found as annual and perennial herbs, vines, succulents, shrubs and tall trees. Members of the Euphorbiaceæ are found in many habitats, from deserts to rain forests. Euphorbiaceae family has genera used as unconventional foods

In Mexico, 826 species under Euphorbiaceae family are found with 50 genera including those introduced from elsewhere and cultivated. A subfamily with a larger number of genera is Acalyphoieae (16), while Euphorbioideae has more species (306), and Crotonoideae has more specific endemism (Martinez-Gordillo et al, 2002). Some of these plants are used in traditional medicine, in oil industry, as ornamental plants, and as food additive. A few examples of its uses include edible wax from Euphorbia antisyphilitica (candelilla) that coats fruits and vegetables to retard water evaporation. Jatropha dioica is a small shrub that is used in traditional medicine and Euphorbia pulcherrima (Poisenetta) an ornamental plant.

B.T.U.: It stands for British thermal unit. The heat required to raise the temperature of one pond of the water through 1oF is called BTU (Plate 82).

Baby back ribs: Meaty pork ribs cut from the lower back rib section.

Bacitracin: A polypeptide antibiotic isolated from a bacillus (Bacillus subtilis or B. licheniformis) and usually used against gram-positive bacteria.

Back Bacon / Canadian bacon: Bacon cut from the loin that has little fat and is cut into round or oblong slices.

Backward feed evaporator: In this evaporator, fresh feed enters the last and the coldest effect and continues on until the concentrated product leaves the first effect (Plate 83).

Fusarium wilt of carnation caused by Fusarium oxysporum f.spp dianthi causes severe yield losses under protected cultivation. For the management of Fusarium wilt, 27 isolates of Bacillus strains were isolated from the rhizosphere of different crops including carnation. Among various species of Bacillus, Bacillus amyloliquefaciens (BSC7) was identified as a most virulent antagonistic bacterial isolate. Studies on antagonism under Environmentally Controlled Scanning Electron Microscopy (ESEM), depicted the presence of rod shaped bacterial cells with biofilm formation at 44847x. Detection for the presence of antibiotic biosynthetic genes reflected the presence of surfactin, iturin, bacillomycin and fengycin genes. The 24 isolates of Bacillus spp. showed positive reaction for surfactin gene. Nineteen isolates were amplified for Iturin A gene except BS5, BS9, BSC1, BSC2, BSC11, BSD1 and BSD2. Strains of B. subtilis BS1, BS2, BS5, BS6 and BS8 expressed Bacillomycin D gene. The genomic DNA of strains BS1, BS2, BS4, BS6, BS7 BS8, BS11 and BS14 showed positive for Fengycin D gene. The isolate of B. subtilis BS2 showed positive reaction for four genes namely surfactin, iturin, bacillomycin and fengycin.

In vitro screening of Bacillus, B. amyloliquefaciens (BSC7), P. aeruginosa (P1) and T. viride (TV1) showed highest inhibition against F.oxysporum f.sp.dianthi to an extent of 55.95, 55.29 and 81.61 per cent over the control respectively. The antifungal activity of antagonistic B. amyloliquefaciens BSC7 and T. viride TV1 against F.oxysporum f.sp. dianthi was confirmed under ESEM by observing disintegration, lysis and structural abnormalities of the hyphae of the pathogen at the zone of interaction.

The application of back-cross breeding first proposed by H. V. Harlan and M. N. Pope in 1922 provides a precise way of improving varieties that excel in a large number of attributes but are deficient in a few key characteristics. F. N. Briggs too started an extensive back-crossing programme to develop bunt resistant varieties of wheat in 1922. As the name implies, the back-cross method of breeding makes use of a series of back-crosses to the variety to be improved during which the character or characters in which improvement is sought is maintained by selection. At the end of back-crossing, the gene (or genes) being transferred will be heterozygous. Selfing at the end of last back- cross produces homozygosity for this gene pair and coupled with selection will result in a variety similar to the well adapted and recurrent parent but superior for the particular characteristic for which improvement programme was initiated.

In back-cross method of breeding, the adapted and highly desirable parent is called the recurrent parent and the source of desirable character being transferred is called as donor parent. It is most effective and easy to apply when the missing trait is qualitatively inherited, dominant and produces a phenotype that is readily observed in a hybrid plant. The procedure for transferring a recessive trait is similar to that for dominant trait but involves an additional step of selfing. Back-crossing is also used to transfer entire sets of chromosomes in a foreign cytoplasm to create a cytoplasmic male sterile (CMS) genotype as has been practiced in maize, onion and wheat, etc. This is done by crossing the donor (of chromosomes) as male until all donor chromosome are recovered in the cytoplasm of the recurrent parent. Back-cross breeding has also been used to develop isogenic lines (lines that differ only in alleles at a specific locus) for traits like disease resistance and plant height, etc.

Globalization of economies and liberalization of trade has led an urgent need for prioritizing the potential areas for investment in order to earn handsome amount of income. Even the entrepreneurs, small as well as marginal farmers aspire to take advantage of global opportunity. Diversification to horticultural crops has been found to be best option as they make more profit, generate additional employment for rural masses and conserve natural resources. Agriculture plays a vital role in the Indian economy. Over 52 per cent of the rural households depend on agriculture as their principal means of livelihood. But unfortunately Indian farmers in general are small and marginal land holders with poor resources and realizing income mainly from cereal based production system, which is insufficient to improve their living standards. Agriculture, along with fisheries and forestry, accounts for one-third of the nation’s GDP and is its single largest contributor (www.indianbusiness.nic.in). GVA at Basic Prices for 2019-20 from ‘Agriculture, Forestry and Fishing’ sector is estimated to grow by 2.8 per cent as compared to growth of 2.9 per cent in 2018-19. The GVA estimates of this sector are based on 1^st advance estimates of agricultural production during Kharif season of 2019-20 obtained from the Ministry of Agriculture & Farmer Welfare. For Livestock sector, estimates of production of major livestock products (i.e. Milk, Egg, Meat and Wool) obtained from the Department of Animal Husbandry & Dairying and Fish production data obtained from Department of Fisheries have been used. The crops including fruits and vegetables account for about 56 per cent, the livestock products 30 per cent and forestry & fisheries 14 per cent share of GVA in total GVA of ‘Agriculture, Forestry and Fishing’ sector [As per the estimates released by National Statistics Office, Ministry of Statistics and programme Implementation, GoI, 2020]. Agricultural exports constitute a fifth of the total exports of the country. In view of the predominant position of the Agricultural Sector, collection and maintenance of Agricultural Statistics assume great importance. In 2018-19, India achieved a record food grain production of 285.17 million tonnes beating the previous year’s (2017-18) 285.01 million tonnes, according to data provided by Department of Economics and Statistics (DES). Also, agricultural profitability has increased over the last decade with record increases in MSPs (minimum support prices) for agricultural produce for all covered crops.