eChapters

Fermentation

The term fermentation is loosely used to denote any process in which microorganisms are involved, regards of whether or not the organism is growing in the presence of molecular oxygen. Thus, it can be defined as 'an ATP generating metabolic process in which organic compounds serve both as electron donor (becoming oxidized) and electron acceptor(becoming reduced). Energetically, fermentations are poor energy yielding process and products have lower energy content then the substrates. Since there is no net oxidation in fermentation, the number of molecules of C, H and O remain the same in products as in the substrate.

Abstract

The increasing interest in polyphenolic compounds in industries has led to the exploration and exploitation of rich bio-compounds from natural sources, essentially plants and their by-products. Solid-liquid extraction is mostly applied for the recovery of these valuable compounds from plants. Since the extraction process is often difficult in a fresh matrix, a drying operation is needed to reduce the moisture content, and preserve the targeted valuable compounds. An uncontrolled drying operation will lead to product damage and hence, loss in quality, reason why, several studies have been carried out to understand and control the drying process. The present chapter aims to present some data on drying for bioactive compound recovery from plants and their by-products, mainly polyphenolic compounds. Our main interest is the mathematical tools used to model the drying process.

Abstract

There are several empirical models to describe deep bed drying dividing the domain into many thin layers. Empirical equations are seen as a resource that only serves to describe the thin layer drying kinetics of an agricultural product. Normally an empirical model is used to determine drying rate. The validity of following thin-layer drying models are accounted by coefficient of correlation (R2), standard error of estimated (SEE), root mean square error (RMSE). The properties of dried foods vary substantially depending upon the type of product, moisture content, drying type and ultimately drying conditions. To take care all above variables, a promising approach is to use Artificial Neural Network (ANN). In process control, the main objectives are food safety, high quality and yield at minimal costs. To obtain high quality products, on-line control techniques are required. Most food processes are highly nonlinear, with time varying dynamics, which complicates food automation. However, the recent developments in artificial intelligence based advanced control tools such as ANN to food processing have opened up novel possibilities for processing industries.

Abstract

Plantbreeding is an ancientart practiced throughselection ofplants fordesirable traits. Over a period of time it has evolved into scientific discipline with various methodologiesbasedon theories and principles ofgenetics. Since the rediscovery of Mendel’s law, there has been accelerated increase in yield of various crop plants due to development of high yielding varieties. Conventional approaches of following phenotypic selection from a variable population in early generation followed by yield testing in later generations have been highly successful in most of the crop species. This coupled with hybrid varieties led to significant increase in food-grain production during later half of the 20^th century. At present manycrops are facing yield plateau asthey have reached their maximum genetic potential. The growing condition is also deteriorating due to changing climate. There is an urgent need among breeders to utilize full arsenal of techniques available to them to sustain crop productivity. Molecular markers and marker assisted selection has come as a boon to the breeders to increase the efficiency ofselection in early generation breeding especially for traits whose phenotypic evaluation is difficult. Further, transgenic technology has the potential to incorporate genes from diverse sources, thus enriching the gene pool from which breeders can search for specific traits. Although at present there is skepticism about transgenic crops among public but this may become a viable strategy in future.

The paddy grain contains a rough outer covering called husk. It accounts for about one-fifth to one - fourth of the weight of paddy. The inner kernel, called brown rice or dehusked rice, again contains some soft outer layers, jointly called bran. It accounts for about 6-8% of the brown rice weight, including the small germ (1-2% by weight) located in one corner. Rice milling is the process of removing the husk and a part of the bran from paddy in order to produce edible rice. Rice milling system ranges from the home-scale to the large, complex modern rice-processing installations. They include hand-pounding equipment, single hullers, emery sheller-cum-huller mills, emery sheller-cum-cone polisher mills and the modern rubber – roller rice mills. Rice milling industry does not only mill rice but it also carries many other essential functions, such as cleaning and grading, drying, storage, quality control etc. for the production of better quality rice. All these processes are discussed in this paper.

Unlike other food grains, rice is mostly consumed as cooked whole grain, Milling technology is therefore geared to obtain maximum outturn of milled rice and to reduce breakage to the minimum. The single huller mills are by and large located in villages or in localities where paddy is custom milled for produces and they still handle the bulk of the country’s production. The large-capacity rice mills located in urban or semi-urban areas for commercial milling are of ½ to 4 tones per hour capacity and handle the rest of the paddy milled. Some battery hullers are still in operation as large commercial mills, specially for milling parboiled paddy.

For Indian foods

Food processing industries are the fastest growing in India. India is the largest producer of milk, bananas, mangoes, guavas, papaya, ginger and okra. It is the second largest producer of wheat, rice, fruits, vegetables, tea, sugarcane and cashew nut. It is the third largest producer of cereals, coconut, lettuce, chicory, nutmeg, mace, cardamom and pepper at the global level (Highlights of Agriculture Census, 2010-11, http://pib.nic.in/newsite/PrintRelease.aspx?relid=132799). Most part of the income is spent on foods. It is important to establish and nurture mutually beneficial relationships with global food processing, food retail and related supply chain organizations. This will in turn help in the realization of significant business growth opportunities in India, through newer technologies, innovations and ways of value additions. The geographical location of India helps in the export of items to other countries. It is connected to Europe, Middle East and Africa from the western coast and to Japan, Singapore, Thailand, Malaysia, Korea, Australia and New Zealand from the eastern coast. Food processing is a priority sector for the Indian Government, especially in recent times with “Make in India” initiative.The country is considered as one of the most favourable markets because of the easy and affordable credit and other fiscal incentives. The total consumption of food and beverages in India is US$ 369 billion which is expected to increase to US$1.142 trillion and the output in the food processing sector might increase to US$ 958 billion by 2025. This offers a vast market opportunity by the Indian food processing, food retail, transport, logistics and related infrastructure sectors to players in the food processing value chain. The market for plant and machinery in the food processing sector is estimated in the year 2024-25 to be USD 51.41 billion as compared to the current value of USD 17.3 billion (2016-17).

Abstract

Tremendous hike inworld population has increased thrust on the limited resources which has considerably affected the viability of the food. The emerging challenge for policy makers and food scientists is to put forward viable solutions. Utilization of underutilized crops/plants that act as good sources of starch could be explored in a better way for the sustenance of the limited sources like food crops/plants. The demand for starch and the foods containing starch has been increasing continually. Since, the starch in its native form has certain limitations in its functional properties to be used for different applications. Therefore, it becomes important to explore certain technologies which could be used for modification of starch. Among different technologies gamma irradiation ensures food safety while inducing physicochemical changes of the components. Although, the chemical modifications have been used widely so far but the concerns related to the chemical residues left are diminishing their demand. Hence, the physical processes particularly the gamma irradiation process has emerged as an efficient alternative with some benefits over other process like eco-friendly, economical, reliable and safe for altering the physico-chemical and biological characteristics of the product. Gamma Irradiation of starch has been successfully employed for the achievement of some desired physico-chemical, biological and functional properties of the foods and other related items. In this chapter, valuable impact of gamma irradiation on the physico-chemical, biological and functional properties of starch has been summarized.

13.1 Biopolymer-Based Coatings with Antimicrobial Properties 13.1.1 Understanding Biopolymer-Based Coatings Biopolymer-based coatings are a game-changer in food packaging, offering natural, sustainable solutions for food safety. These coatings, derived from renewable sources, like polysaccharides (e.g., chitosan, starch, cellulose), proteins (casein, gelatin), and lipids (wax, shellac), have proven to be biodegradable and consumer-friendly alternatives to synthetic polymers. 13.1.2 Antimicrobial Properties and How They Work • Antimicrobial coatings are crucial in slowing down spoilage and extending the shelf life of perishable foods. They work by disrupting cell membranes, inhibiting cell processes, and creating oxidative stress in microbes. This is achieved through natural antimicrobials like essential oils (e.g., oregano, thyme, clove), plant extracts, and organic acids; inorganic agents like silver nanoparticles, zinc oxide, and titanium dioxide; and microbial-derived substances like nisin, lysozyme, and bacteriocins (Yu, Z et al ., 2022)

Studies have shown that modified atmosphere packaging (MAP), and controlled atmosphere storage have the ability to delay quality losses and thus extend shelf life of fresh or minimally processed or fresh-cut produce (Church 1994; Gil et al. 1996a, b; Lee et al. 1995). Modified atmosphere packaging of fruits can result in reduction of respiratory activity, delay in softening and ripening and a reduced incidence of various physiological disorders and pathogenic infestations (Artés 1993).

9.1. Introduction

In a fast-paced world, demand for pre-cooked, fresh-like, and convenient products without preservatives is increasing. This demand has triggered the need for better alternative packaging materials for food products like MAP (Modified Atmosphere Packaging), CAP (Controlled Atmosphere Packaging), edible packaging, biodegradable packaging, etc. Packaging plays a crucial role in transport, distribution, storage, retailing, and end-use, a means of ensuring safe delivery to the ultimate consumer in sound conditions at optimum cost. It performs the functions depicted in Fig.1 for both fresh as well as processed products (Opara et al., 2007; Opara, 2009).

Introduction

Improvement of wheat grain quality depends on the knowledge of quality requirements of different end-use products and the genetic components controlling different quality traits. In previous chapters we have dealt in-depth with quality requirements of different end-use products and their genetic components. Depending upon the goal of quality improvement, different strategies are adopted to improve wheat quality. It depends upon the number of genes controlling the traits as well as the availability of the simple methods for phenotyping the traits especially during early segregating generations. In addition stability analysis of the traits across the environments is essential before we go for breeding. Constraints in time, cost, and sample availability usually necessitate the use of predictive tests during early generations, followed by milling and baking tests on advanced lines that have met a number of agronomic, disease and predictive quality criteria. Knowledge of germplasm lines having specific quality traits is needed to initiate breeding for the improvement of wheat grain quality for different end-use products. The quality requirements of different major end-use products are provided in the following table (Table 1).

Abstract

The conventional methods of pathogen identification have often depended on identification of disease symptoms, isolation and culturing of the organisms, and identification by morphology and biochemical tests. The major limitations of these culture based morphological approaches, however, are the reliance on the ability of the organism to be cultured, the time consuming nature and requirement of extensive taxonomic expertise. Furthermore, diagnosis of plant diseases can be even more difficult with asymptomatically infected propagative materials such as tree grafting stocks or potato tubers. The use of molecular methods can circumvent many of these shortcomings. Accordingly, there have been significant developments in the area of molecular detection of plant pathogens in the last three decades. The polymerase chain reaction (PCR) which revolutionised molecular diagnostics and biological sciences. In the last decade the range of targets that can be diagnosed using diagnostic PCR have grown tremendously. The very flexibility and application specific variations in the basic theme of the system have allowed the development of many PCR variants adapted to wide range of applications. Furthermore, diagnostic PCR has been greatly improved by the introduction of the second generation PCR, known as the Real time PCR where closed-tube fluorescence detection and quantification during PCR amplification (in real time) is possible eliminating the need for laborious post-PCR sample processing steps which greatly reduces the risk of carryover contamination. Using Real Time PCR, it is possible not only to detect the presence or absence ofthe target pathogen, but it is also possible to quantify the amount present in the sample allowing the quantitative assessment of the number of the pathogen in the sample. Enumerating the pathogen upon detection is crucial to estimate the potential risks with respect to disease development and provides a useful basis for diseases management decisions. Crops can be attacked by many pathogens which, in addition, often occur in complexes. Therefore, many disease diagnostic applications require simultaneous detection and quantification of several targets. Methodological limitations, however, are in many cases the reason for developing simplex or assays designed for only few targets. The DNA Microarray technology, originally designed to study gene expression and generate single nucleotide polymorphism (SNP) profiles, is currently a new and emerging pathogen diagnostic technology, which in theory, offers a platform for unlimited multiplexing capability. It is viewed as a technology that fundamentally alters molecular diagnostics. The fast growing databases generated by genomics and biosystematics research provides unique opportunity for the design of more versatile, high-throughput, sensitive and specific molecular assays which will address the major limitations of the current technologies and benefit plant pathology.

Abstract

Molecular markers play key role in crop improvement. There are severalmarkers such as RAPD, SSR, AFLP, SNP, DArT and many more are available for their practical uses. From the past markers like RAPD, AFLP, and SSR have been used extensively in the crop breeding. Recently new markers such as SNP and DArT have become popular among the researchers of molecular biology for use in genomics-assisted crop breeding. These markers have been applied for study of genetic diversity, germplasm management, cultivar identification, genetic mapping, gene tagging and genomics etc. Though the conventional method of breeding willcontinue to play a primary role in the crop improvement, molecular marker technology is becoming one of its integral components. Agarose-based DNA markers like ISSR, CAPS, RAPD, SCAR and AS (allele specific) have the potential for faster and accurate breeding for target traits through MAS.

A. Morphological Examination of Bacterial Samples
Introduction
Bacteria are most widely distributed, the simplest in morphology, the smallest in size and difficult to identify. They are prokaryotic and a few are photosynthetic. Most bacteria are only 0.5 to 2.0 μm in diameter. Bacteria are generally rod, spherical or curved shaped. Rod shaped bacteria may vary considerably in length, have square round or pointed ends and may be motile or non-motile. The spherical or coccus shaped bacteria may occur single, in pairs in tetrads, in chains or in regular masses. The helical and curved shaped bacteria exist as slender spirochetes, spirillum and bent rods (vibrios). In this exercise the ubiquitousness of these organisms will be demonstrated in the air and water, on body and in mouth etc.
Materials
1. Petri plates containing nutrient agar
2. Sterile cotton swabs
3. Sterile water blank
4. Tube of nutrient broth
5. Materials for simple staining

Bacteria are morphologically different. This characteristic is helpful in the identification of bacteria. Routine study of bacterial morphology should include examination of stained preparations. Motility should be examined by hanging drop preparation. According to the requirement, certain cell structures may be stained. Stained preparations should be made preferably from fresh agar slant cultures. Smears of Streptococcus should be made from a liquid culture. Morphology of bacteria may be described under the following terms:

1.Shape: Spherical, coccobacillary, short or long rods, filamentous, comma- shaped, curved or spiral forms.

2.Size: Spherical are measured in diameter and rod-shaped in length and breadth. Measurement is expressed in microns (µ).

3.Sides: Straight, concave, bulging, parallel or irregular.

4.Ends: Round, pointed, truncate or concave.

5.Axis: Straight or curved.

6.Arrangement: Singly, in twos, in groups of four or eight, in grape-like bunches, in long chains, or scattered irregularly, may be in bundles, angular arrangements or in Chinese letters.

7.Pleomorphism: Bacteria may differ from their typical shape. Some of the older cultures may depart greatly from their typical forms, and may show spindles, clubs, and filaments, swollen and branching forms. Variation may be both in shape and size.

8.Capsule: May or may not be present. When present, size should be noted. This requires special staining.

Abstract

Multiflash drying (MFD) process is based on the application of multiple cycles of heatingvacuum pulse to a solid food. Heat can be provided by convection (from hot air), conduction (by contact with a hot surface), microwaves, or by a combination of these methods. Due to the moderate temperatures applied to the drying food, and the sudden decompression step (vacuum pulse), MFD processes allow obtaining crispyanddried foods with very good nutritional and sensory properties. Vacuum pulses cause food texturization, which depends on the pressure drop rate during decompression. In fact, the dried products are similar to freezedried foods, but with more open structures, which result in higher crispness. The dehydration can be performed in relatively short processing times, when compared with traditional drying processes (e.g. solar drying, airdrying, vacuum drying and freezedrying). The short process time indicates clearly that this class of drying process presents a huge potential to the production of driedandcrisp foods at industrial scale.

Mushrooms are most ancient, best known and greatly admired fleshy macrofungi. Mushrooms are delicacies, much sought after and greatly valued foods. Wild mushrooms are mostly found in forests, tree trunks, pastures, lawns and road side during winter season and appear at surprising speed. Mushroom hunting or gathering is a popular hobby in Western countries. The wild mushrooms may be edible or unpalatable or poisonous. Poisoning by wild mushrooms is common and may be fatal or produce merely mild gastrointestinal disturbance or slight allergic reaction. The term toad stool is popularly reserved for inedible or poisonous mushrooms, but this classification has no scientific basis. This alarming term keeps many people away from these foods. It is important that every mushroom intended for eating be accurately identified. Mushrooms vary in colour, size, shape, growth habit, yielding ability and other characteristics.

STRUCTURE: Mushrooms are the fleshy fungi which constitute a major group of lower plant kingdom. Mushroom is the conspicuous umbrella-shaped fruiting body (sporophore) of certain fungi of the order Agaricales in the class Basidiomycetes but also of some other groups. Popularly, the term mushroom is used to identify the edible sporophores. The edible reproductive part consists of a stem and a cap. Gills found on the underside of the cap, are arranged like spokes in a wheel. The gills bear multiple spores which start new fungi. The sporophore emerges from an extensive underground network of fine thread like white growth known as mycelium. The mushroom, fruit body produced basidiospores at the tip of club like structures, called basidia, which are arranged along the gills of the mushroom. Beneath the mushroom, in the soil, is the mold colony itself, consisting of a mat of intercoiled hyphae, sometimes several feet in diameter. The mushrooms first appear as white tiny ball consisting of short stem (stipe) and a cap (pileus) which begins to open up like an umbrella. The delicate membrane or Veil (velum) enveloping the cap tears off, if allowed to develop fully, and lamellae (gills) radiating from the stalk into the cap become visible. These gills become darkened as the basidiospores (seeds) develop into millions and fall in the ground for starting their life cycle once again for second generation of mushrooms.

1. Introduction

Mushrooms represent a highly specialized group of fungi, mostly basidiomycetes, with production of fruiting bodies bearing the spores for continued propogation. The fruiting bodies vary greatly in shape, size, color, texture, structure and aroma (Rajarathnam et al., 1998). They are endowed with the unique ability to biodegrade cellulosic wastes, including lignin, and biotransform into edible fruiting bodies (Rajarathnam et al., 1992, 1998, Rajarathnam and Zakia Bano, 1990). Their chemistry is also varied comprising characteristic polysaccharides, phenolics, proteins, amino acids, peptides, lectins, fatty acids, minerals, and so on (Rajarathnam et al., 1998). The wide spectrum of compounds have endowed the mushroom species with a spectrum of biofunctional activities of medicinal importance and accordingly their role in human health are implicated through studies on chemical, cell culture and animal feeding experiments. Vast literature available on this subject is condensed for presentation here.
Fifty nine medicinal species are reported from Diaoluoshan Nature Reserve in Hainan Province, China (Li et al., 2003). A health food made from luminescent mushrooms (Mycena chlorophos) is described. The luminescent parts that emit light in the carpophore of the mushrooms are dried without thermal decomposition and pulverized (Sugimoto, 2006). Consideration is given to the health promoting properties of 3 types of Asian fungi, namely, shiitake (Lentinus edodes), reishi (Ganoderma lucidum) and maitake (Grifola frondosa). Properties considered include beneficial components (e.g polysaccharides, vitamins, amino acids) and effects on the immune system (Anon, 1997). Auricularia auricula (kikurage or tree-ear) and Tremella fuciformis (shirokikurage or white jelly-leaf) are widely used in Chinese cuisine. Chemical structures of some of the polysaccharides from A. auricula and T. fuciformis, in relation to their medicinal and nutritional effects are described (Misaki and Kakuta, 1995).

1. INTRODUCTION

Mushrooms are edible fungi of commercial importance and their cultivation has emerged as a promising agro-based land-independent enterprise. There are about 20 varieties of mushrooms being cultivated throughout the world for food. In India, only white button mushroom (Agaricus bisporus J.E. Lange Imbach), oyster mushroom (Pleurotus spp. [Fr.] P. Kumm) and paddy straw mushroom (Volvariella volvacea [Bul.] Singer) are grown commercially. Of these, white button mushroom contributes ~90% of the total production. Mushrooms have been recognized as an alternate source of good quality protein and produce the highest quantity of protein per unit area from agro-wastes. Besides, they provide potentiality for generating employment, improving economic status of growers, help in checking pollution and earn foreign exchange.

Out of the total production about 45% of mushrooms produced are consumed in the fresh form while the other 55% are processed, with 5% in dehydrated form and 50% in canned form. This is because their shelf-life in the fresh form is very short and hence mushrooms are traded in the world market mostly in the processed form. Fruiting bodies (reproductive phase) of Agaricus are appreciated not only for texture and flavour but also for their chemical and nutritional characteristics.

Abstract

Mycotoxins are naturally occurring chemicals produced by fungi growing on grain, feed or food. The fungi that produce mycotoxins in grains commonly include Fusarium, Aspergillus and Penicillium species. The toxins can accumulate in maturing corns, cereals, soybeans, sorghum, peanuts and other food and feed crops in the field and in grain during transportation. The toxins may occur in storage under conditions favorable for the growth of the toxin producing fungi. Diseases in animals and human beings resulting from the consumption of mycotoxins are called Mycotoxicoses. These fungal metabolites are detrimental to the health of both animals and human. Toxicity ranges from acute death to chronic diseases, cancer and reproductive malfunction. Mycotoxin contamination of food and feed grains is a serious economic problem for grain producers and processors because grain contamination impacts market 

Fungi cause diseases in plants and animals and in such diseases, the fungi actively grow and invade the body of their hosts. There is another means by which fungi can cause harm is growing on stored food material that we consume and produce toxic metabolites referred to as mycotoxins (means fungus poisons). Food is particularly susceptible to fungal infections at almost every stage of storage or production. Mycotoxin is toxic substance produced by a fungus. The term is restricted to toxins produced by fungi growing in human foods or animal feeds. The growth of the fungi in food grains leading to physical spoilage of grains and toxin production is a serious problem in developing countries. Their occurrence in food and feed has both economic and health implications, as the toxic effects in human beings, animals and other living systems were well established. The effects of poisoning by mycotoxin are referred to as mycotoxicoses.

Mycotoxins are naturally occurring toxins produced by certain molds and can be found in food. The molds grow on a variety of different crops and foodstuffs including cereals, nuts, spices, dried fruits, apples and coffee beans, often under warm and humid conditions. Mycotoxins can cause a variety of adverse health effects and pose a serious health threat to both humans and livestock. The adverse health effects of mycotoxins range from acute poisoning to long-term effects such as immune deficiency and cancer. A scientific expert committee jointly convened by WHO and the Food and Agriculture Organization of the United Nations (FAO), called JECFA, is the international body responsible for evaluating the health risk from natural toxins including mycotoxins. International standards and codes of practice to limit exposure to mycotoxins from certain foods are established by the Codex Alimentarius Commission based on JECFA assessments.

Mycotoxins are toxic compounds that are naturally produced by certain types of molds (fungi). Molds that can produce mycotoxins grow on numerous foodstuffs such as cereals, dried fruits, nuts and spices. Mold growth can occur either before harvest or after harvest, during storage, on/in the food itself often under warm, damp and humid conditions. Most mycotoxins are chemically stable and survive food processing. Several hundred different mycotoxins have been identified, but the most commonly observed mycotoxins that present a concern to human health and livestock include aflatoxins, ochratoxin A, patulin, fumonisins, zearalenone and nivalenol/deoxynivalenol. Mycotoxins appear in the food chain as a result of mold infection of crops both before and after harvest. Exposure to mycotoxins can happen either directly by eating infected food or indirectly from animals that are fed contaminated feed, in particular from milk.

Naïve panel: It refers to the sensory evaluation panel that has not been trained specifically regarding the product evaluation being undertaken in the study. It is untrained panel of sensory evaluation.

Nano technology: It is the art of manipulating materials on an atomic or molecular scale to build microscopic devices. The word “nano’ meaning ‘dwarf” in Greek language refers to dimension / magnitude of the material / particle in the order of 10-9. Nanotechnology is nothing but manipulating of particles into very small particles called nano-particles. These particles have dimensions in the scale of nano-meter and used to design / build materials and devices. Nanotechnology has been provisionally defined as relating to materials, systems and processes which operate at a scale of 100 nanometers (nm) or less. It will allow many things to be manufactured at low cost and with no pollution. The concept of nanotechnology was first given by Nobel Laureate physicist Richard P. Feynman in Southern California in 1959.

NaNO2 : Pink colour of cured meat is preserved by NaNO2 . It is effective against Cl. Botulinosm

Nanogram: It is one billionth of a gram.

Nano-materials: Nano material have been defined as having one or more dimensions measuring 100 nm or less, or having at least one dimension at this scale which affects the materials behavior and properties (Plate 438).

Fundamentals of Nanotechnology in Packaging • Overview of Nanotechnology in Packaging: Nanotechnology in packaging refers to using materials manipulated at an extremely small scale (1-100 nanometers) to boost the performance and capabilities of packaging. Techniques like nanoparticles, nanofibers, and nanocomposites are integrated into packaging materials to improve their physical properties, prolong shelf life, and introduce new functions like antimicrobial features. Mechanisms and Key Materials • Nanoparticles: Small particles added to packaging to enhance strength, f lexibility, and barrier properties. Common types include silver, titanium dioxide, and zinc oxide nanoparticles. • Nanofibers: Made from substances such as chitosan, cellulose, or polymers, these fibers increase strength and are useful in active packaging coatings. • Nanoclays: Dispersed in polymers, these increase resistance to gases and moisture, enhancing barrier performance

Introduction

The mankind in the past two centuries has witnessed several technology revolutions: industrial, agricultural, medical, info-tech, biotech etc. Nanotechnology is recent one in the series. Nanotechnology is the manipulation or self-assembly of individual atoms, molecules, or molecular clusters into structures to create materials and devices with new or vastly different properties. It uses techniques, processes and materials at the supramolecular level, approximately in a range between 1-100 nanometres (nm), in order to create new properties and to stimulate particular desired functionalities. For the food industry, nanotechnology applications include: release systems for pesticides or fertilizers in agriculture; antibacterial or easy-to-clean surfaces in food processing machines; food additives such as anti-caking in salt, powders and coffee creamers; anti-foaming agents for beer; natural colour additives for beverages; encapsulated nutraceuticals(antioxidants, vitamins) for dietary supplements; and, micelle systems for low-fat foods. It is predicted that in the foreseeable future nanotechnology will transform the entire food industry, changing the way food is produced, processed, packaged, transported, and consumed. The word “nano,” derived from the Greek “nano” signifying “dwarf,” is becoming increasingly too vast in terms of its huge potential in agriculture, health and the environment. In the agricultural sector, nanotech research and development is likely to facilitate next stage of development of genetically modified crops, farm and animal protection and production inputs, food processing, nutraceuticals etc. India is bestowed with a variety of crops, vegetables, fruits, flowers but food sector is handicapped due to inefficiencies in production, processing, storage and packaging. Most of the processing and packaging in industry and agriculture leaves behind a trail of environmental hazards, which if not tackled at this juncture, will add to long term ecological problems.

Fruits and vegetables are perishables and the post-harvest losses from the point of production at the farm gate till it reaches the consumers is estimated as 30-35 per cent which constitute a major economic drain of more than 33 billion USD equivalent to Rs 2,40,000 crores annually. In order to reduce the losses at various stages, nanotechnologies have come as potential strategies to minimize the losses while improving the marketability and profitability of perishables. One of the plants derived natural compounds “hexanal” which is known to extend the shelf-life of fruits when it is used as a pre-harvest spray or post-harvest dip as a nano-emulsion. Since, it is a highly volatile compound, efforts were undertaken to entrap the compound in the polymer-based nano f ibres (as nano stickers) or as a cyclodextrin inclusion complex (as nano pellets) in order to preserve the perishables. Further, hexanal vapour was also evaluated to preserve fruits. In this book chapter, various nanotechnologies tested, evaluated and technologies developed over the years are elucidated. Overall, the literature review suggests that use of any of the nanotechnologies delivered as pre-harvest spray, post-harvest dip, nano-stickers, nano-pellets and vapour singly or in combination found to reduce the infestation of post harvest diseases, minimize the losses and extended shelf-life by 2-3 weeks that help the farmers to get lucrative prices due to the late arrival in the market besides significantly improve the productivity and profitability of orchards.

Introduction

Off late our understanding of the composition and functions of the human gut microbiota has increased exponentially and there is a great interest in producing healthy foods in particular that which have an impact in improving the gastrointestinal health such as probiotics, prebiotics and synbiotics. Many of these functional foods are dairy based and have been accepted greatly worldwide. Nonertheless, there has been a need for the development of nondairy probiotic, prebiotic and synbiotic products (Salmeron 2017). This has prompted food scientists and food industrialists to study the feasibility of applying other fermenting substrates such as cereals for the development of innovative nondairy fermented functional foods. The importance of including nanoscience and nanotechnology techniques for the creation of fermented cereal beverages that contain specific bioactive nanoparticles and their role in food packaging systems will be discussed.

7.1 Introduction

Food processing has become one of the most competitive sectors in recent years. With increasing consumers’ demands, food industry needs to keep updating technology. These efforts are focussed at improving the organoleptic quality and nutrition of food and improve its keeping quality. This drives the industry to look for newer technologies which include new products as well as new processes. Some of the new processing technologies include non-thermal processes such as high pressure processing, pulsed electric fields and ultrasound processing. Advanced thermal processing techniques such as ohmic heating, inductive heating, extrusion and infrared heating have also been developed. Innovative methods for improving food quality through irradiation, removal of heat and moisture from food are now available. Newer packaging techniques now help to improve shelf-life and attractiveness of the food products and cater to the biodegradability of the packaging material. Hence, there is abundance of new products that have now become part of our daily life.

Food nanotechnology has its long history when the first step of revolution in food processing and improvement in quality of foods was introduced by Pasteur to kill the spoilage bacteria (1000 nm). Later, Watson and Crick’s model of DNA structure, which is about 2.5nm, opened the gateway of applications in biotechnology. Nanotechnology is defined as the design, production, and application of structures, devices, and systems through control of the size and shape of the material at the nanometer (10^-9 of a meter) scale. Nanotechnology is one such technology that has the potential to revolutionize the food industry. Taniguchi first used nanotechnology in 1974 to describe production technology at ultrafine dimensions. The word “nano” means “dwarf” in Greek and one nanometer is equivalent to 10^-9 m. Objects of this scale are not visible through the human eye or the ordinary optical microscope. A nanometer (nm) sized particle measures one billionth of a meter. A human hair measures 80,000 nm while a DNA strand is 2.5 nm wide whereas a protein chain is 5nm in diameter. Nanotechnology can be applied to develop nanoscale materials, controlled delivery systems, contaminant detection and to create nanodevices for molecular and cellular biology. Nanotechnology may be defined as the understanding and management of matter at dimensions of roughly 1-100 nanometers. Unique behavior of matter at the nanoscale enables novel applications. Nanoscale science, engineering, and technology can be used for imaging, measuring, modeling, and manipulating matter at this length scale. Range of sizes of nanomaterials in the food sector and their relative position on nanoscale/microscopic scale are listed below.

Nanotechnology-based preservation employs materials generally measuring between 1 and 100 nanometres. Their high surface-area-to-volume ratio, adjustable surface chemistry and responsiveness to external stimuli enable highly controlled interactions with foods and microorganisms. Nanomaterials may deliver antimicrobial compounds, antioxidants and other active substances in a controlled or targeted manner. Silver, zinc oxide and chitosan nanoparticles can be incorporated into coatings and films to reduce microbial contamination while maintaining sensory and nutritional quality. The chapter discusses nanoencapsulation, nanoemulsions, antimicrobial nanoparticles and nanosensors for detecting spoilage and monitoring shelf life. It also considers effectiveness, controlled release, food applications, safety assessment and potential regulatory concerns associated with nanotechnology.

Introduction

The act of substituting food or drink with low grade quality ingredients thereby the f inal product will fail to the meet the regulation or standards and in most cases even unfit for human consumption (Augustin et al., 2009). With demand for profit based manufacturing market, there will be increase in points of adulteration with cheaper alternatives or inferior grade ingredients in the production of food or drink (Banti, 2020)]. Adulteration is highly prevails in developing countries such as India, where about 70% of milk supply is adulterated on day to day basis (Bittar et al., 2017). It is every nation and each individual’s responsibility to provide safe and nutritious food to the consumers.

Previous Year Questions

1. Write down the various programmes run by state & central govts for ensuring food security in our country. Briefly discuss the strategies for sustainable agricultural production (20M, CSE 2023)

2. How will national food policy help in sustainable food security? (CSE 2020, 10 marks)

3. Discuss the national initiatives for food and nutrition security. (8M, IFoS-2019)

4. What are the special schemes for providing food grains to underprivileged, destitutes and malnourished populations (CSE 2016, 12.5 marks)

5. National and international food policies. (10M, IFoS-2016)

Introduction

The much awaited new educational Policy 2020 approved and released by the Central Government on July, 29, 2020. A document which is set to transform Indian education system in most holistic way and will exert a long lasting influence on framing the Indian education system by addressing the vivid issues across the discipline and streams. The implementation of this policy will be under a single body HECI (Higher Education Council of India) well supported by multiple bodies like National Higher Education Regulatory Council(NHREC), Meta-accrediting body referred as Nation accreditation Council (NAC), Higher Education Grant Council (HEGC), General Education Council(GEC) also called Graduate attributes, it will formulate the National Higher Education Qualification Frame work (NHEQF). This council will take over presently regulating bodies of higher education like University Grant Commission, All India Council of Technical Education and National Council of Teachers Education.

Various methods are employed to dry flowers and foliage. The method adopted for drying depends largely on the character of the plant, that is to say whether the plant is glabrous, succulent or not succulent, hardy or delicate in nature. The suitable method for drying any given plant has to be studied elaborately. However, the different methods of drying are described broadly in this and consequent chapters.

The major drawback of dried arrangements and other dry flower products is that they are dull and lack the brightness of fresh flowers. To overcome this problem, flowers are bleached and dyed. This enhances the visual appeal of the product. Both chemical and natural dyes can be used. Squires (1971) reported on the use of various types of paints to colour dried foliage and flowers using brushes or by dipping and spraying. Enamel paints, interior paints, poster paints and tube paints could be employed for this purpose. Tampion and Reynolds (1971) explained the three ways by which dyeing can be carried out for fresh flowers. They are as follows:

1. Introduction

Antioxidants are considered an important class of nutraceuticals which are associated with health improving properties. Antioxidants are compounds which when present in foods at concentrations lower than an oxidizable substrate, markedly delay or prevent the oxidation of substrate (Halliwell, 1999). They are increasingly applied in food systems to retard the oxidation process, particularly in lipid containing foods (Droge, 2002; Lee et al., 2004). The commercially available solutions to counter food oxidation are predominated by synthetic antioxidants such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and tertiary butyl hydroquinone (TBHQ), among others, which are increasingly rejected by consumers and legitimized by regulators due to long term health implications (Park et al., 2001). As a consequence, the development and utilization of natural antioxidants as alternatives to synthetic ones have attracted global interest among researchers.

The use of spices and herbs in foods was known since ancient times to modify or to improve the flavor. However, the antioxidant properties of only some of the spices have been recognized. Different spices and their extracts such as turmeric, ginger, fennel, rosemary, and sage, among others, are reported to possess very high antioxidant properties, owing to the content of biologically active compounds, which enables their food application (Madhavi et al., 1996). Exploitation of natural antioxidants in consumer goods is increasingly accepted and will gain momentum due to the rise of green consumerism. In this chapter, an attempt has been made to provide an insight into natural antioxidants and colors. Beginning with the source distribution of natural antioxidants, followed by review of extraction methods for their isolation from plant based matrices and bio-preservative properties in vegetable oils to delay rancidity. Lastly, a thorough collection of literature pertaining natural colors has been presented to potentiate the application of clean label ingredients in food systems. The application of natural antioxidants and colors in food products can vouch the food safety with value addition in terms of their nutraceutical properties.

Introduction

Fruits contain various beneficial compounds that possess biological activities and can be used as pharmaceutical nutraceutical products. Most of the fruits contain polyphenolic components that can promote antioxidant potential on human health. Wild Blueberries, strawberries, plums, oranges, grapes, cherries and mangoes are rich in antioxidant. Additionally, anti-inflammatory, antibacterial, antifungal and chemopreventive effects are associated with the various fruits. Besides polyphenols, other compounds such as xanthones, carotenoids, saponins also exhibit health effects. Fruit consumption is considerably increasing in the daily diet because they supply high levels of biologically active compounds that impart health benefits beyond basic nutrition. Fruits provide an optimal mixture of antioxidants such as vitamins C and E, polyphenols and carotenoids, vitamins, phytoestrogens, glucosinolates and anthocyanins. Diets rich in fruits may help to delay the ageing processes and reduce the risk of certain types of cancer, cardiovascular diseases and other chronic diseases. The combination of vitamins, minerals, phenolic, antioxidants and fibre seems to be responsible for these effects. The most dietary components in fruits acting as antioxidants are fibre, polyphenols, conjugated isomers of linoleic acid, D-limolene, epigallocatechin, gallate, soy protein, isoflavones, vitamins A, B, C and E (group of tocopherols), calcium, selenium, chlorophyllin, alipharin, sulphides, catechin, tetrahy-drocurecumin, seasaminol, glutathione, uric acid, indoles, thiocyanates and protease inhibitors.

Introduction

Pulses are annually grown leguminous crops that yield one to twelve grains/ seeds within a pod, and are often promoted in diet owing to their low cost and many beneficial nutritional effects (Craig 2009). The United Nations general assembly has declared 2016 as the international year of pulses (Oyeyinka et al. 2016). The Food and Agriculture Organization (FAO) describes pulses as the crops that are harvested only for dry grain. This definition excludes crops that are harvested green as food sources (such as green beans and green peas), crops that are used only for sowing purposes (such as alfalfa and clover) and crops used primarily for oil extraction (such as peanuts and soybean). India is the largest producer (around 25%) of pulses in the world and the Indian subcontinent covers the leading area in terms of consumption of pulses. It was also suggested that India can also increase the production output by 30%, considering a rise in government support prices for stimulating the growth of pulses and in case of a normal monsoon season (FAOSTAT 2008). The mainly grown pulses in India include mungbean (green gram), chickpea (Bengal gram), urd bean (black gram), lentil (masur), pigeon pea (arhar), cowpea, horse gram and peas (Tiwari and Singh 2012). In the western countries, nowadays pulses are one of the preferred food sources of the population, owing to ideological reasons and interest in vegetarian diet.

Introduction

Functional elements are non-conventional biomolecules in foods that have the ability to modulate one or more body metabolic processes or pathways, leading in health advantages and well-being promotion. Research has demonstrated a connection between food, health and well-being functional elements. Consequently, functional elements have health-promoting roles connected with multiple progressive steps at various phases of disease control, from initiation to growth. They can therefore be implemented efficiently in disease therapy and prevention. Therefore, the borderline between food and medicine is becoming very thin at a moment when the role of a healthy diet in stopping non-communicable diseases is well recognized. Functional elements include plant-derived, non-nutritious and biologically active chemicals that operate in the body to avoid certain non-communicable diseases from occurring.

Food contains over 900 phytochemicals. One fruit or vegetable serving (about 120g) can contain as many as 100 distinct phytochemicals. Previously, functional elements were believed to happen predominantly only as phytochemicals in plant products including whole grains, fruits and vegetables. However, in animal goods such as milk, fermented dairy products and cold water fish, probiotics, conjugated linolenic acid, long-chain omega-3, −6 and −9 polyunsaturated fatty acids and bioactive peptides are also discovered. Usually, functional elements happen in various forms in food such as glycosylated, esterified, thiolated or hydroxylated materials. They also have numerous metabolic operations that enable positive impacts in the body on several illnesses and target tissues (Balic et al. 2020).

Introduction

Society is dynamic. The requirement of society varies in course of time. During the life process human being faces lots of health hazards which are mostly due to intake of various foods. So people become cautious about the intake of food and think about the quality of foods which can protect the health of human being. In ancient times people made medicines from plant extracts to protect their health. In 1989, Dr. Stephen coined the term “Nutraceuticals” which is combination of nutrition and pharmaceutical. Nutrition is required for health and pharmaceuticals provide remedy for sickness. So it is a preventive approach. Nowadays, the definition of nutraceuticals has been expanded to include vitamins, minerals, herbs and other botanicals, amino acids, and dietary substance for human use as a supplement diet (Stauffer, 1999).

Nutraceuticals are dietary supplements made only from whole foods to augment health. Currently over 470 nutraceutical and functional food products are available with documented health benefits (Brower, 1999). The patients are much aware about the side effects of chemical agents in short and long term therapy. So to avoid medicine used for small diseases, new therapies came into trend with the help of nutritional approaches i.e. “Nutraceuticals”. The use of nutraceuticals becomes easier than pharmaceuticals since nutraceuticals does not require any animal verification for its use in human. Nutraceuticals,

Functional foods and Dietary Supplements are most important concept in food science. They have distinction amongst themselves.

1. INTRODUCTION

Wine is an ancient fermented product as revealed by the wine jugs recovered (Plate 1) during excavation from different places. Wine is made all over the world and wine produced in each region from different parts of the world has different characteristics. Development and implementation of practices and policies for sustainable growth of grapes and other fruits is the most essential component for the growth and development of wine industry. The science dealing with wine production, evaluation and consumption is known as 'Oenology'. For the introduction of wine, several aspects influence the wine quality are style, the consumer perception and finally, the marketing are taken in account. Factors related with fruit growing, ripening, micro organisms and other verification factors need to be considered in wine production.

Abstract

Though India is second largest producer of fruits and vegetables but is far behind in per capita availability of this protective food as compared to many developed countries. Floriculture and allied horticulture crop production (spices, medicinal & aromatic crops, mushroom etc.) have great potential to generate high income, jobs and new careers. Hi-tech horticulture is the need of all the developing countries and it refers to “the precise production techniques for efficient use of inputs at appropriate time and quantity for maximization of yield and quality of crops”. Various ways to make horticulture hi-tech include modern nursery management including micro propagation, protected crop production, high density planting including canopy management, use of agrochemicals, rejuvenation technique, interated nutrient management, integrated water management, integrated pest and disease management. Inspite of this, in present context, major emphasis has been given on clean horticulture production with bio-derived, renewable energy sources for developing a profitable CEA (controlled environment agriculture) that would help in sustaining the system, improving socio-economic status of growers and of-course environment-friendly.

Abstract
 
Technological advancement, increased urbanization, awareness and interest of consumer for variety products have given a great challenge to the food sector to develop inexpensive foods that are nutritionally superior and acceptable to the intended consumers. Keeping this objective in view, we have made an attempt to develop technology for variety products from wheat and corn and suitability of available varieties judged. With the emerging trends of food market towards fast foods, there are vast prospects for efficient utilization of cereals by exploring varieties products. A steamed bread (a Chinese traditional product) and Tortilla and corn chips (Mexican snacks) were developed. Steamed bread is defined as the product resulting from cooking or steaming fermented dough. Recipe and method for its preparation was standardized. Nutritionally rich steamed bread can be prepared by supplementing 10% level of chickpea and defatted soybean flour. Corn snacks have become a prime target of some consumer advocates and nutritionists who have referred them as a source of ‘empty calories’. But reality is that these snacks provide instant energy, as they are rich storehouse of nutrients. Process and recipe for the preparation of Tortilla and corn chips from Indian maize were standardized. The quality and acceptability of these snacks were worked out and found that these products were highly acceptable. Indian consumers have versatile taste, so these products will be readily accepted in Indian market. 

1.    UV irradiation is considered better than other irradiation methods in terms of

(a) Physical structure maintenance

(b) Chemical structure

(c) Sensory quality

(d) Microbial quality

2.    Irradiation with UV light is considered as

(a) Sterilization method

(b) Disinfection method

(c) Pasteurization method

(d) Cooking method

3.    UV treatment kills bacteria, viruses, fungi due to its effect on

(a) Metabolic pathway

(b) DNA

(c) Signaling pathway

(d) ATP generation

4.    Effectiveness of UV radiation on micro organisms depends on

(a) Species and strain

(b) Spore formation

(c) Culture and growth

(d) Both (a) and (c)

Abstract

Ghee means the pure clarified fat derived solely from milk or curd or from desi (cooking) butter or from cream to which no coloring matter or preservative has been added. It is the second largest dairy product prepared from milk and it is almost 35% of total milk production. Unfortunately, the producers or the middle- men involved in the ghee trade, tend to adulterate ghee with cheaper oils and fats like vegetable oils, animal body fats, hydrogenated fats and sometimes even the non-edible mineral oils, especially during lean season to earn more money. Recently, the problem of adulteration has assumed a very serious dimension. Such a situation has tarnished the image of dairy industry. Methods presently adopted by food law enforcing agencies to ensure the quality of ghee are mainly based on the physico- chemical constants, but all these methods have some limitations. So researchers are always in search of different innovative methods to check the quality of ghee.

Food technologies now have more sweeteners from which to choose than ever before. With the obesity epidemic and the public attention it currently receives, plus the increasing interest in foods with added benefits sweeteners that allow for consumer appealing labeling are of particular interest. These sweeteners fall into basic categories, those that are essentially calories free, often referred to as low-calories or intense sweeteners, and those, which are significantly reduced in calories, which may be referred to as reduced calories-sweeteners, bulk sweeteners, or sugar replaces. These sweeteners when used alone or in combination may permit such labeling as ‘low-calories’, ‘reduced-calories’, ‘light’ ‘sugar-free’ and ‘dose not promote tooth decay’.

The ideal sweeteners does not exist, it would be at least as sweet as sucrose and provide the same properties to a product as sucrose, with processing parameters similar to those of sucrose so that existing equipment can be used. It would be colourless, odourless and noncariogenic, with a clean, pleasant taste and have immediate onset and not much lingering. Solubility and stability are important. The ideal sweetener must be compatible with a wide range of food ingredients because sweetness is but one element of a complex food flavour system. Even sucrose is not perfect, being unsuitable for some applications.