eChapters

1. Introduction

Intermediate moisture foods are semi moist foods that have some of their water bound by glycerol, sorbitol, salt or certain organic acids, thus preventing the growth of many microorganisms (Stewart et al. 2001). It is now well recognized that fruits and vegetables are dehydrated to an intermediate moisture level (20-50%) (Stewart et al. 2001; Purvi et al. 2003). As the intermediate moisture products are characterized by a semi moist consistency so these foods have enough moisture content to permit easy chewing but low enough water content to prevent spoilage production of intermediate moisture foods is based on an increased scientific understanding of the chemical reactions involved in traditional food preservation methods (Purvi et al. 2003).
There will be better retention of original flavor and texture compared to fully dried products, with concomitant reduction in bulk, weight and cost of packaging, transportation and storage. Such intermediate moisture foods are more appropriate for developing countries in view of their minimal processing requirements, stability under ambient conditions, safety, convenience, ease of nutrient content adjustment, energy savings and low capital investment. They are also eminently more suited than dehydrated or canned foods for military rations (FAO, 2005).

From the earliest time, man had to conserve and preserve his food, so that surplus at harvest following a successful crop can be stored for use throughout the year. Prime agricultural products in general and more so with fruits and vegetables which are being processed world wide or preserved to a more stable form so that they can withstand transport, handling and storage over a long period. As civilization developed, man learnt to preserve fruit and vegetable products with salt, spices and by drying , smoking or cooling which could extend their shelf life. Later by experience, he learnt that by protecting it from environmental factors such as air, moisture and light, the shelf life of these processed products could be further increased. He tried to keep them dry and covered to protect from insects and rodents. As with solid foods, primitive man experimented with beverages also. It is likely that the first beverage known to him after water was fruit juice. This undoubtedly led to the discovery of wines made from the fermented fruit juices. Later on, preservation methods and development of packaging materials underwent drastic changes.

1. Introduction

Extrusion is one of the most preferred ways for creating the indulgence called ready-to-eat (RTE) snack foods with diverse shapes, sizes, colours and also nutritional and functional values which appeal to the diverse group of consumer with varied choices. In this chapter ready-to-eat extruded and puffed snacks are the focus covering the technology involved, criteria for ingredient selection, design of experiment, process modelling, effects of extrusion on physical, nutritional and functional quality of the snack; a few relevant case studies are also discussed.

1.1 Market for RTE snack foods

Indian cooking and lifestyle have undergone tremendous changes in the last 15 years. There are many major factors impacting this change which include, liberalization policy, dual income, separate living of couples, innovative kitchen applications, media proliferation, etc. The cooking style and eating habits in India varies drastically from southern part to northern part of the contry. Due to lifestyle pressure nowadays people prefer easy short way of cooking food rather spending too much time on cooking. Rapid urbanisation is also one of the reasons for people to buy such products. Snack foods comprise a very large variety of items including potato chips, crackers, nuts and extruded snacks, among others (Harper, 1981). Snack food extrusion includes subjecting selected raw material to a variety of complex physical processes to yield snacks with varied shapes and textures (White, 1994).

The RTE market in India was expected to expand to reach Rs. 2,900 crore by 2015, according to an analysis done by Tata Strategic Management Group (TSMG). The products may vary from noodles, pasta, masala packages, stuffed paratha, etc. and many more. There are many regional players who are making foray into ready-to-eat segment. Many national and international players like Nestle, Pepsico, ITC, Haldirams, Cavinkare, are already in Indian market with their products.

1. Introduction

Milk and milk products constitute essential dietary components for a vast majority of the population. India with annual production of more than 135.6 million tonnes has emerged as leading milk producing nation. The per capita per day availability of milk in India is more than 290 g. Buffalo milk accounts for 57% of the total milk produced in India. Ethnic dairy products account for 90% of all dairy products consumed (Aneja et al., 2002). Many health benefits have been attributed to fermented dairy products and probiotic microorganisms (Salminen et al., 1998). The market for functional foods, nutraceuticals, wellness foods and beverages is fast growing. The harmful effects of synthetic drinks have propelled health conscious consumers to go for natural and traditional drinks. In search of better returns, the Indian dairy industry is widening its focus to include traditional milk products, and these are emerging as new profit centres for the organised sector.

Traditional dairy products have several advantages. They require low capital investment and give high profit margin. They are more appealing to the Indian palate. Dahi or curd is the most popular indigenous fermented dairy product. There are numerous references to dahi in vedic literature. The Prevention of Food Adulteration Act (Anon, 1976) describes dahi as a product obtained from pasteurized or boiled milk by souring (natural or otherwise), using harmless lactic acid or other bacterial cultures. Indian dahi and its western counterpart yoghurt differ considerably in terms of cultures used and their bouquet and flavour profiles (Aneja et al., 2002). The major flavor compound in dahi is diacetyl. Dahi made from buffalo milk has a firm body and white glossy appearance, with a cream layer on top. Mixed mesophilic cultures of Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp diacetylactis along with Leuconostoc species are grown together for production of dahi.

1. Introduction

There is a general consensus that fruit and vegetable consumption is a key factor for a balanced diet. Globally, majority of people consistently are consuming less than the daily recommended fruit and vegetable requirement. Even in developed nations e.g., Australia, Canada, Europe, UK and USA, researchers have concluded that there is large gap between actual and recommended consumption of both fruits and vegetables despite decades of concern and publicity while resultant outcomes were short-lived. In a study from 52 low and middle-income countries 77.6% of men and 78.4% of women consumed less than the minimum recommended servings of fruits and vegetables. Same study reported 74% low fruit and vegetable consumption among adults in India (Hall et al., 2009). In India, recent studies on the fruit and vegetable intake amongst urban residents was reported as 265 g per day as against 400 g per dayof fruits and vegetables excluding potatoes and other starchy tubers, that is recommended by the World Health Organization (WHO, 2008).

Epidemiological studies conducted in recent years have indicated a positive correlation between the consumption of fruits and vegetables and prevention of cancer and cardiovascular diseases. Health-promoting functional characteristics of vegetables are mainly attributable to their functional bioactive constituents. These effects can be listed as antioxidant activity, cholesterol-bindingcapacity, antidiabetic activity and angiotensin-converting enzyme (ACE) inhibitory activity. Studies conducted in cell culture models and animal models have revealed that bioactive compounds of plant foods, including soybean, hawthorn fruit, tea, buckwheat, oats, onion, garlic, almond, and vegetable juice, composed of tomato watercress, parsley, carrots, celery, lettuce, beets and spinach, have cholesterol-lowering activity (El-Shatanovi et al., 2012; Vadivel et al., 2011).

1. Introduction

Antioxidants are considered an important class of nutraceuticals which are associated with health improving properties. Antioxidants are compounds which when present in foods at concentrations lower than an oxidizable substrate, markedly delay or prevent the oxidation of substrate (Halliwell, 1999). They are increasingly applied in food systems to retard the oxidation process, particularly in lipid containing foods (Droge, 2002; Lee et al., 2004). The commercially available solutions to counter food oxidation are predominated by synthetic antioxidants such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and tertiary butyl hydroquinone (TBHQ), among others, which are increasingly rejected by consumers and legitimized by regulators due to long term health implications (Park et al., 2001). As a consequence, the development and utilization of natural antioxidants as alternatives to synthetic ones have attracted global interest among researchers.

The use of spices and herbs in foods was known since ancient times to modify or to improve the flavor. However, the antioxidant properties of only some of the spices have been recognized. Different spices and their extracts such as turmeric, ginger, fennel, rosemary, and sage, among others, are reported to possess very high antioxidant properties, owing to the content of biologically active compounds, which enables their food application (Madhavi et al., 1996). Exploitation of natural antioxidants in consumer goods is increasingly accepted and will gain momentum due to the rise of green consumerism. In this chapter, an attempt has been made to provide an insight into natural antioxidants and colors. Beginning with the source distribution of natural antioxidants, followed by review of extraction methods for their isolation from plant based matrices and bio-preservative properties in vegetable oils to delay rancidity. Lastly, a thorough collection of literature pertaining natural colors has been presented to potentiate the application of clean label ingredients in food systems. The application of natural antioxidants and colors in food products can vouch the food safety with value addition in terms of their nutraceutical properties.

Introduction

The tropical root and tuber crops are comprised of crops covering several genera. They are staple foods in many parts of the tropics, being the source of most of the daily carbohydrate intake for large populations. These carbohydrates are mostly starches found in storage organs, which may be enlarged roots, corms, rhizomes, or tubers. Many root and tuber crops are grown as traditional foods or are adapted to unique ecosystems and are of little importance to world food production. Others such as cassava (Manihot esculenta Crantz) and white-fleshed sweet potato (Ipomoea batatas L.) are known worldwide. Several of these crops have been termed under-exploited and deserving of considerably more research input

Potato and Banana chips: Potato chips are one of the popular snack foods consumed throughout the country. Chips are mostly prepared by small scale artisans / confectioners, and sold in plastic film or paper packs. Recently, however some of the companies have installed plants having relatively large capacities and have started marketing chips in attractive packs. The fat content generally ranges between 25-40% and moisture content between 2-4%. Potatoes having high total solids, low reducing sugars and low amylase activity are most suitable for chips manufacture.

Banana chips are prepared from varieties rich in starch having very low levels of soluble sugars and polyphenol - oxidase activity. These are mostly processed and marketed in Southern States especially in Kerala. Previously, the chips were fried in coconut oil, but now other vegetable oils are also used in their processing. Relatively, banana chips are harder in texture than potato chips.

    1.    As per FSSAI, 2011 regulations, what is the fat and SNF content in buffalo milk
        a)    5 & 9    b)    6 & 8
        c)    6 & 9    d)    None
    2.    As per FSSAI, 2011 regulations, what is the fat and SNF content in  cow milk
        a)    4 & 9    b)    4 & 8.5
        c)    6 & 9    d)    None
    3.    As per FSSAI, 2011 regulations, what is the fat and SNF content in  standardized milk
        a)    4 & 9    b)    4.5 & 8.5
        c)    6 & 9    d)    None

Abstract

Though India is second largest producer of fruits and vegetables but is far behind in per capita availability of this protective food as compared to many developed countries. Floriculture and allied horticulture crop production (spices, medicinal & aromatic crops, mushroom etc.) have great potential to generate high income, jobs and new careers. Hi-tech horticulture is the need of all the developing countries and it refers to “the precise production techniques for efficient use of inputs at appropriate time and quantity for maximization of yield and quality of crops”. Various ways to make horticulture hi-tech include modern nursery management including micro propagation, protected crop production, high density planting including canopy management, use of agrochemicals, rejuvenation technique, interated nutrient management, integrated water management, integrated pest and disease management. Inspite of this, in present context, major emphasis has been given on clean horticulture production with bio-derived, renewable energy sources for developing a profitable CEA (controlled environment agriculture) that would help in sustaining the system, improving socio-economic status of growers and of-course environment-friendly.

1.    Major difference between unleavened and leavened bakery products is

(a) Unleavened do not form cracks on surface

(b) Unleavened do not rise significantly during baking

(c) Leavened bakery products are not attractive

(d) Unleavened products are much more stable

2.    Unleavened bakery products contain small holes throughout the finished product because of

(a) Ingredients contain fats that may swell

(b) Air incorporation between cells

(c) Incomplete coalescence of mixed ingredients

(d) High temperature baking

6.1 Introduction

The food industry produces large volumes of waste both solid and liquid, starting from the initial step of raw material to the final consumption of processed food. Apart from a loss of valuable biomass and nutrients, the generated waste poses increased disposal and potentially severe pollution problems. Meat and meat industry is no exception where slaughter houses, meat processors, wholesalers and rendering plant generates huge amount of waste [1, 2].

Meat and meat processing industry, is among the least profitable industries despite its immense size and large gross sales. Effective recovery and utilization of by-products and wastes is the lifeline of meat and poultry processing industry. Thus, it becomes imperative that an effort be made to reduce expenses by employing the use of new or modified processing methods and through in-plant treatment, where waste effluents and by-products could be recovered and often upgraded to useful products of higher value. Meat processing wastes have a potential for recycling raw materials or for conversion into useful products of higher value as by-product or even as raw material for other industries or for use as food or feed after biological treatment.

It is well known that lowering the temperature of food stuffs reduces microbiological and biochemical spoilage and thus extending the shelf life of the product. In addition, it is generally agreed upon that the reaction rate is reduced by half by lowering the temperature by 10°C. Though low temperature preservation process has been recognized as a preservation technique for several hundred years, the major developments in its utilization have occurred only in the last century.

10.1 Introduction

Food safety and quality are the two important factor which results in consumer food choices. Nowadays consumers are demanding food which is safe and of high quality in terms of nutrition due to a healthier life style. Food safety and quality are the terms used interchangeably. Food safety refers to all those hazards, that make food injurious to the health of the consumer and it is not negotiable. Food quality includes attributes that influences the product's value to the consumer. This includes both negative and positive attributes; negative attributes includes spoilage, contamination, discoloration, off-odours and positive attributes include colour, flavour, texture and processing method of the food. This distinction between safety and quality has implications for public policy and influences the nature of the food control system. Food control is defined as a mandatory regulatory activity which provides consumer protection and ensures food is safe, wholesome and fit for human consumption during production, handling, storage, processing, and distribution.

Despite the huge efforts made by the food safety authorities and industries, food safety still remains a critical issue. In food industries, producers have to satisfy both safety and quality criteria for their products to the consumer. Food processors have multiple options in terms of different quality and management systems, they have to choose the most appropriate one for its specific activity and should establish, document and implement effective systems in the processing area for managing quality and safety of the product. Over the past few decades, food-borne illnesses have raised great concern about the safety of food. Considerable steps are taken by the food processor for understanding and managing the risks of airborne diseases. Among various systems, Hazard Analysis and Critical Control Point (HACCP) is effective tool which focuses mainly on controlling hazards to ensure the production of safe and wholesome food. For maintaining the safety and increasing the shelf life of food, traditionally it has been preserved by thermal processing, freezing, salting and drying. But nowadays consumers are demanding convenient, innovative, fresh foods, including new "minimally processed" products. To meet consumers' expectations in the 21^st century, the food industries are implementing novel technologies whose purpose will be two fold: 1) to provide the new quality attributes demanded by consumers; and 2) to ensure the all important and often expected assurance of food safety. The novel technologies will include various minimally processed technologies like high pressure processing (HPP), pulsed electric field (PEF), ohmic heating, dielectric heating, microwave heating, ultrasound, modified atmosphere packaging (MAP) and cleanroom technology. Among these cleanroom technology have attracted great attention for maintaining the safety and quality of the food products as because it doesn't have any effect on the nutritional quality of the food being processed, it maintains the hygienic condition in the processing area, remove contaminants from the processing area and clean air in the processing area which in turn results in safe food product. The main objective of cleanroom technology is to eliminate airborne contaminants from the humans and/or from the environment. An air curtains and a positive air pressure is maintained in the processing unit using filtered air. Production personnel will use the special clothing which is preferred for cleanroom to reduce the contamination from both air- and human-borne microorganisms. In this chapter we will discuss about cleanroom technology, its historical background, its classification, layout of cleanroom, air handling, personnel and its clothing, regulations in cleanroom, and its application in food industries.

1. EXPERIMENT 1 : INDUSTRIAL USES OF IMPORTANT CHEMICALS

Introduction

Baking is a traditional activity which has a long history, and according to some recent discovery, it is indicated to be dated back to as early as around 21,000 B.C. It is termed as a complex operation of prolonged heat transfer through radiation, convection, and conduction. Baking is considered to be one of the oldest methods of cooking, most commonly the bread baking but it is not only limited to the bread instead it provides a wide range of products such as cookies, pastry, pizzas, breakfast cereals, etc. Baking includes lots of raw materials such as flour, water, yeast, milk and milk derivatives, shortening, sugars, eggs and some minor ingredients such as malt products, cocoa products, dough improvers, inhibitors of microbiological spoilers, spices, etc. Food items which are rich in sugar and carbohydrates such as chocolates, candies are known as confections, and the art of making these products is termed as confectionary. Confectionery can be broadly classified into two major categories, namely sweet confectionary and baked confectionery. Conventionally cake was defined as the flour based sweet food, whereas bread was defined as the flou-based non-sweet food.

Baked confectionery, also known as flour confectionery includes products such as cakes, pastries, and similar baked goods, especially which are served in dessert. These delicacies hold history not much later to the bread baking. Earlier the baked confectionery especially cakes were only affordable by the rich and were prepared for special occasions such as festivity, celebrations, etc. but now a large number of flour confectioneries are being produced in the world which is delightfully acceptable and are much more in demand by a various group of people.

Introduction

Food additives are substances of natural or synthetic origin, which are added to foods to serve a certain technological or sensory function. They are utilized in the preparation and processing of almost all types of food in order to give favorable attributes to the food. Additives are the substances which are of little or no nutritive value but are added to food to enhance its flavour, appearance or other favourable quality. They are used in the processing or storage of foods especially in the developed countries includes antioxidants, food preservatives, colouring and flavouring agents etc. Food additives are substances added to food to preserve flavour or enhance its taste and appearance (Latiful Bari and Yeasmin 2018). Some additives have been used for centuries for example, preserving food by pickling with vinegar, salting, as with bacon, or using sulfur dioxide as in some wines. The use of food additives is not a modern-day invention; they have been used for centuries. The practice probably started when man first discovered that fire would cook and thereby preserve his meat. Later he realized that the addition of salt would preserve without cooking. Food preservation began when man first learned to safeguard food from one harvest to the next and by the salting and smoking of meat and fish. The Egyptians used colours and flavourings and the Romans used spices and colours for preservation and improving the appearance of foods. Thus, food additives have been used for centuries to enhance the variety of food products, to preserve, improve its taste and appearance, and provide a better mouth feel. Food additives afford us the convenience and enjoyment of a wide variety of appetizing, nutritious, fresh, and palatable food. Their quantities in food are small, yet their impact is great.

A wide variety of fungal and bacterial pathogens cause postharvest disease in fruits and vegetables. Some of these infect produce before harvest and then remain quiescent until conditions are more favourable for disease development after harvest. Other pathogens infect produce during and after harvest through surface injuries. In the development of strategies for postharvest disease control, it is imperative to take a step back and consider the production and postharvest handling systems in their entirety. Traditionally fungicides have played a central role in postharvest disease control. However, trends towards reduced chemical usage in horticulture are forcing the development of new strategies. Controlling or reducing disease relies on integrated crop and postharvest management, with attention to fungicide application, crop hygiene and nutrition, and the management of ripening, to optimise advantages conferred by the plant’s natural resistance factors that prevent and delay disease development. Agricultural production and food distribution systems have evolved over many years to a complex system which allows for nearly year-round supply of most fresh commodities through longterm storage and long distance shipments to consumers. The expectation of year round supplies has resulted in increased challenges for postharvest handling systems. The availability of fungicides for disease management likely played a major role in the evolution of our current system of food distribution (Mitcham, 1999). With the recent loss of registration of many postharvestfungicides and more expected under the Food Quality Protection Act (FQPA), as well as consumer pressure to reduce the amount of chemicals used on food, the challenge to maintain our current system of food distribution increases.

Saccharides: A monosaccharide sugar or combination of sugars is called saccharides.

Saccharification: The process of breaking a complex carbohydrate (starch or cellulose) into its monosaccharide components is called saccharification.

Saccharimeter: A device (polarimeter) used for measuring the amount of sugar in a solution is called saccharimeter. It is also known as saccharometer. It is a hydrometer with a special scale.

Saccharin: A crystalline compound C7 H5 NO3 S that is unrelated to the carbohydrates, is several hundred times sweeter than sucrose, and is used as a calorie free sweetener.

Saccharomyces: It is one of the genus (Saccharomyces) of the family Saccharomycetaceae and is usually unicellular yeasts (brewer’s yeast) that are distinguished by their sparse or absent mycelium and reproduced asexually by budding.

Safe food: Food that provide maximum nutrition and quality while revealing a minimal hazard to public health and they assume any risk present to be minimal.

Safety: Safety is a component of quality. Many experts believe that food safety is the most important quality component since a lack of safety can result in serious injury and even death of the consumer.

Abnormal milk: It is milk that is not smooth and homogenous but is flaky, ropy, stringy, slimy, watery, discoloured or bad flavoured (Plate 1 & 2).

Abrassive peeler: It is equipment comprising a rotating drum lined with carborandum (an abrasive material) in which certain vegetables like potato and ginger are tumbled and exposed to the abrasive action of the carborandum lining, thereby facilitating separation of peel from the vegetables (Plate 3).

Absinthe: It is green liqueur which is flavored with wormwood, anise, and other aromatic herbs (Plate 4). Its commercial production is banned in ma ny countries for health concerns.

Absolute alcohol: It is water free alcohol. It contains atleast 99 per cent pure alcohol by weight and obtained by digesting rectified spirit over quicklime and redistilling the same.

Absolute density: It is the ratio of weight of certain volume of a substance at 1oC to that of weight of same volume of water at 4oC.

Absolute humidity: Absolute humidity is defined as kg of water vapour per kg of dry air at a given temperature and pressure. Absolute humidity is the mass of water vapour divided by the mass of dry air in a certain volume of air at a specific temperature. The warmer air absorbs more water than cooler one. Absolute humidity is the measure of water vapour or moisture in the air, regardless of temperature.

Absolute pressure: It is equal to atmospheric pressure minus vacuum pressure (Plate 5).

Absolute zero: It is a theoretical temperature characterized by complete absence of heat and motion and equivalent to exactly -273.15°C or -459.67°F (Plate 6).

Introduction

The purpose of this e-book is to outline the main hazards in food production and processing that could influence the safety of foods and thus the health and wellbeing of consumers. The suggestive actions to restrain the formation of compounds or organisms that may threaten the safety of a food will be discussed along with the legal and regulatory framework.

“Safe Food Now for a Healthy Tomorrow”

Food is the third most basic human need, after air and water. Only when food is safe can it contribute to our food security and health. When food is not safe, people cannot thrive, hunger and poverty cannot be alleviated, and a healthy life is not possible. Food safety has a significant impact on human health, and food, which is essential for human survival, comes from plants and animals. Food safety is a core tenet of One Health.

This chapter gives an overview of the main hazards such as physical hazards, chemical hazards, biological hazards, and adulterants in different types of foods and how the hazards influence the safety of the food along with health and well-being of the consumers. The effective way of management of these hazards and different hazard limits set by the regulators such as FSSAI, USFDA, EU AND CODEX along with labelling requirements for the hazards has been discussed in this chapter.

Introduction

The cold chain is a meticulously managed series of actions using specialized equipment to ensure products remain within a specific low-temperature range from production to consumption (ASHRAE, 2024; IIR, 2024). This unbroken sequence encompasses refrigerated production, storage, and distribution activities, safeguarding the safety and quality of perishable and thermo-sensitive goods like agricultural commodities (such as fresh produce, meat, dairy, seafood, chemicals, pharmaceuticals, flowers, wine, etc.), processed foods and pharmaceuticals (Aung and Chang, 2014; Awan, 2023). For climacteric fruits like bananas, custard apples, apples, pears, tomatoes, mangoes, and avocados, cold storage facilitates controlled ripening, extending the time between harvest and consumption. Cold chain management, integral to the overall supply chain, requires expertise in maintaining temperature, air quality, and adherence to regulatory standards. Any disruption in this process can lead to product spoilage and financial implications (FAO, 2019). The management of the cold chain system is crucial for preserving the freshness, taste, and nutritional value of fruits from harvest to consumption (Kader, 2003).

Horticulture sector, comprising fruits, vegetables, floriculture, medicinal and aromatic crops, mushroom, spices and plantation crops, had witnessed phenomenal growth in production and productivity during the last two decades. Resultantly, horticulture in India is recognised as one of the fastest growing sectors in the world. The production has reached to 351.92 million tonnes in 2022-23 as compared to 231 million tonnnes in 2010. Progressive urbanization, increased spendable income and awareness for balanced and nutritious food have contributed towards increasing demand of horticulture produce. The increasing demand coupled with higher income returns per unit area has triggered this growth pattern, which has been largely possible due to policy environment to support horticulture both for research and development.

Coconut, arecanut and palmyrah, which hold significant cultural and historical importance in India, have been the primary focus of cultivation efforts. Cocoa, a relatively recent addition to India, serves as a companion crop to coconut and arecanut, contributing to the economic well-being of farmers nationwide. However, the current situation emphasizes the crucial role of value addition and product diversification in stabilizing prices. Despite the initial stages of value addition in India’s palm crops, recent innovations and established value chains have the potential to empower farmers as entrepreneurs, thereby augmenting their income. Moreover, the value-added products from these palms have enormous export potential. This chapter explores the advancements in value-added products and the diversification of coconut, arecanut, palmyrah, and cocoa, providing insights into opportunities for cottage or small-scale industries.

Fruits and vegetables are one of the most consumable commodities globally and also serves as an important ingredient in human dietaries. There are a lot of different varieties of fruit and vegetable and the prices keep fluctuating of the products. The production of fruit and vegetables needs to be handled, processed, or utilized properly. The costs involved in preventing the losses are always cheaper than the cost of production, therefore processing receives higher attention in recent years. The fruits and vegetables processing industry was earlier highly unorganized but due to the increasing demand for processed
food, the industry has started attracting investors. India facilitates the fruits and vegetables processing industry in obtaining its primary resources; India is the second-largest producer of fruits and vegetables. The global fruit and vegetable processing market includes the global fruit & vegetable processing equipment market and processed fruits & vegetables market. There are different methods included in fruits and vegetable processing that helps in increasing the life of seasonal fruits and vegetables are canning, dehydration, freezing, pickling, etc.
Crystallized Fruits
Candied fruit when covered or coated with crystals of sugar, either by rolling in finely powdered sugar or by allowing sugar crystals to deposit on them from dense syrup are called crystallized fruits.

INTRODUCTION

Radiation processing is a promising technology for improving hygiene and increasing storage and distribution life of food products. Ionizing radiation can be used to bring about beneficial changes in food stuffs (Urbain 1995) and it has been suggested as a method of ensuring safety of meat products (Patterson and Stevenson 1995). Ionizing radiation interacts with an irradiated material by transferring energy to electrons and ionizing molecules by creating positive and negativities (Moseley 1989).

Carbohydrates are synthesized from CO₂ and H₂O in chlorophyll containing plants during photosynthesis. The term carbohydrate was originally coined for this class of compounds as most of them were hydrates of carbon or could be represented by the general formula C_n(H₂O)n. Not all the carbohydrates however, have this empirical formula, for example deoxyribose (C₅H₁₀O₄) and glucosamine (C₆H₁₃O₅N). Further lactic acid (C₃H₆O₃) formaldehyde (HCHO or CH₂O) and acetic acid (CH₃­.COOH or C₂H₄O₂) with this general formula is not a carbohydrate. Carbohydrates are defined as the compounds having either an aldehyde or a ketone group or a modified aldehyde or ketone group and other carbon atoms with alcoholic (-OH) group. Hence the continued usage of the term carbohydrate is for convenience rather than exactness.

    1.    Cereals are grown over 73% of the total world harvested area and contribute over 60% of the world food production providing dietary fibre, proteins, energy, minerals,  vitamins, b-glucans , Oligosaccharides, such as lactulose, fructo-oligosaccharides, transgalacto-oligosaccharides

    2.    Finger millet is a potent source of antioxidants and has potent radical-scavenging activity that is higher than that of wheat, rice, and other millets; these results corresponded to their phenolic content

    3.    The brown or red variety of finger millet, which is commonly available, had higher activity (94%) than the white variety (4%) using the DPPH method.

    4.    Most of the herbal plant polyphenols are known to have multi functional properties such as reducing agents, hydrogen donating antioxidants and singlet oxygen quenchers and flavonoids and their derivatives are the largest and most important group of polyphenols.

    5.    Phytosterols are found in plant foods and are structurally and functionally analogous to cholesterol in vertebrate animals. a-sitosterol, campesterol and stigmasterol are the most commonly occurring phytosterols and constitute 95% of total phytosterols in the diet.

1. Introduction

At present there is an increasing demand for colourants from natural sources. Since there is a possibility of banning of synthetic colours in view of their carcinogenic properties and already a reduction in number of permitted synthetic colours from 13 to 8 numbers in our legislation (PFA, 2005), the demand for natural colours is evident. In Japan and European countries because of the green movement, the demand for synthetic colours has been reduced. Synthetic colours in global market account for 400 million dollars. Despite processing limitations and higher costs, the global market for natural colours is estimated to be more than 939 million dollars (Espszezola, 1998). Color is recognized as a major factor affecting food acceptance. The consumer expects a specific food to possess a well defined colour and rejects any appreciable deviation from the normal. The change in colour during the preparation of food is a useful guide to quality control and is used by many food processors as the criterion for selecting raw materials. The characteristic colour of raw food is due to the natural pigments present in the plant and animal material. These can be enhanced in processed food products through the addition of food colourants. Addition of colour can make up for the colour losses during processing. Some of the major groups of colours are anthocyanins, carotenoids, betalains, chlorophylls, flavones and chalcones. Some of the plant sources of pigments are kokum, roscelle, chilli, marigold, beetroot, grapes and safflower. Sources of natural colours can be plants, animals or microbes. Fruits and vegetables form one of the main plant sources of pigments. Some of the plant materials rich in colours viz., alfalfa, marigold, safflower, strawberry, aronia fruits, grapes, red cherries have been exploited for pigment extraction and the results have shown positive effects. Some of the interesting features of the work carried out by researchers in this area are presented in the following sections.

1.    What is Surimi?
    
2.    Fisheries Resources for Surimi Production

    
3.    Historical Perspective of the Surimi Industry
    
4.    Present World Scenario of Surimi Industry

5.    Surimi Industry in India
    
6.    Processing Technology for Surimi from Light Muscle Fish 
    6.1     Types of fish used for surimi
    6.2     Heading, gutting, and deboning
    6.3     Mincing
    6.4     Washing and dewatering
    6.5     Stabilizing surimi with cryoprotectants
    6.6      Rapid freezing and packaging
    
7.    Surimi Processing from Dark Muscle Fish
    
8.    Quality Evaluation of Surimi
    
9.    Microbial Quality of Surimi
    
10.    Surimi based Products
    10.1     Fish cakes
    10.2     Fish balls
    10.3     Crab analogs (Crab leg meat analog)
    
11.    Scallop Analogs
    
12.    Moulded Products
    
13.    Important Considerations in Surimi and Surimi based Products
    13.1    Gelation
    
14.    Functional Properties of Surimi

15.    Cryoprotectants in Surimi
    15.1    Mechanism of action of cryoprotectants
    
16.    Future Development in Fish Protein Stabilization
    
References

1.    In milk and milk products, non bacterial ropiness or sliminess may be due to the stringiness/sliminess caused/resulted

(a) Due to thin film formation of proteins, when cooled

(b) From cream thickness

(c) By mastitis, fibrin and leucocytes from the milch animal’s blood

(d) All of the above

2.    In milk, bacterial ropiness is caused by slimy capsular material from the cells usually

(a) Lipids

(b) Proteins

(c) Gums or mucins

(d) Polysaccharides

10.1 Introduction

Edible oil production is a separate sector of the food processing industry. There are several species of plants in the world whose oil can be utilized for human consumption for edible and non-edible uses. Though around 40 oilseeds are known, only around a dozen oilseed crops have become commercially important including ten seed crops and three tree crops. These oil seed crops (mostly annual) of commercial importance are groundnut, rapeseed-mustard, sesame, soybean, sunflower, cottonseed, safflower, niger, castor and linseed. The three major tree oil crops are palm, olive and coconut [63]. Besides these, the kernels of some other tree crops i.e. apricot, almond, walnut, hazelnut, olive (fruits are used), peach, can also be utilized for oil extraction purposes for edible and non-edible uses. Besides oil of the oilseed crops, the oils of tree fruits and kernels are also becoming popular very fast for various food, pharmaceutical and cosmetic industries.

Large scale requirement and local shortages of oils for various edible and non-edible uses have prompted the identification of many new sources of oil during the last few decades. They are ricebran, corn, sal, mahua, jatropha, kokum, melia, rubber, mango kernel, kusum, simarouba, neem, karanj, crambe, moringa, tumba, jojoba and tobacco seed etc. Most of these oilseeds and their products have wide utility in the specialty oils sector and are mostly used for non-edible purposes. Soaps and detergents, paint and varnish, lubricants, pesticides and medicines, cosmetics, bio-diesel fuel industry are the major consumers of these non-traditional oilseeds. All the oil crops will gain greater attention in future as they are renewable and biodegradable in nature [63]. List of oil crops have been given in Table 1.

1.1 Importance of Food Process Engineering

Food occupies the first position among the hierarchal needs of human beings. It is more important than shelter and clothing. After production and be forec on sumpti on, food commodities are subjected to nume rous unfavourable physical, chemical, microbial or parasitic factors which may cause their spoilage or disease when consumed. To prevent such spoilage, a process engineer has to understand these and provide remedial measures. The two primary goals of processing operation are first to minimize post harvest losses and second value addition of raw food commodities through preservation and processing.

Present level of production of different types of food commodities and their estimated post-harvest losses along with monetary values are given in Table 1.1. It shows that various food items get lost, to the tune of 65 million tonnes, worth Rs. 76,000 crores per annum, during post-harvest phase. A substantial amount of these losses could be prevented if appropriate agro processing centers, having backward linkage with farmers to ensure constant supply of quality raw food materials, are established and operated. Profit generated through value addition must also be shared with farmers, who are only people in the world to create wealth every year, in the form of food, fiber and other commodities necessary for human survival. It can bring sea change in rural areas, where 65-70% population live, in respect of economy, health and happiness.

It sounds simple, but the above process is fairly complex and requires lot of coordination in order to succeed. The major challenge is to minimize the losses during the various post harvest operations and keep the food as fresh as possible. In order to do so, one must first try to understand the various causes of post harvest losses of food commodities and the various factors that influence theses causes. Secondly, we must use those food processing operations that will not only extend the shelf life of the produce but also convert the raw food commodities to more acceptable product to the consumer.

3.1 Introduction

The subject of heat transfer is firmly grounded in the sciences of thermodynamics and fluid mechanics. It has become exceedingly important for engineers to possess a clear understanding of the principles of heat transfer and its application to a large number of problems. In view of process engineering, the knowledge of heat transfer and its application is most important, because heat transfer is one of the basic unit operations in the agricultural processing activities. The design of process equipments, refrigerators, boilers or type of heat exchangers, thermal treatment of food grains, cooling of perishables, pasteurizations, sterilizations, freezing of milk and fruit juices and a large number of similar applications involve consideration of heat transfer rates. There may be other situations where heat is being generated during a certain operation, and may be detrimental to the equipment and the food product. In such cases we are often faced with the task of removing the undesirable amount of heat as far as possible.

4.1 Introduction

The purpose of processing the food is to make it safe and healthy for human consumption. The term food processing refers technologically anything, which transforms raw ingredients into consumer ready products, with the aim of stabilizing food products by preventing or reducing deteriorative changes in quality. The objective of food manufacturers is to develop, modify and employ those processing technologies that retain or create desirable sensory qualities in food and eliminate or reduce undesirable changes due to processing.

The purpose of food processing is to increase the shelf life of food before spoiling (preservation); to remove, destroy or inhibit pathogens and toxins (food safety); to change flavor, texture, aroma, color or form (variety); to reduce preparation times and make food more portable (convenience); to restore and/or raise nutrient levels in food (nutrition).The various food processing methods used extensively in food industry include physical (high temperature, freezing, chilling, dehydration, packaging), chemical (control of storage gases, reduction of water activity, pH, addition of chemical additives or spices) preservation methods.

Traditionally, food preservation has been associated with thermal treatments alone or in combination with biochemical or chemical methods. For example, the thermal processing of milk is done various thermal treatments viz. at 63^oC for 30 min for low temperature long time (LTLT), 72^oC for 15 seconds for high temperature short time (HTST) pasteurisations and up to 140^oC for a few seconds for ultra high temperature (UHT) treatment. However, such thermal treatments effectively reduce level of microorganisms but in long-term preservation, it may lead to losses of desired organoleptic attributes and destruction of temperature liable nutrients and vitamins. The limitations involved with thermal processing has been overcome by employing chemical preservation, cool chain management and process hygiene in the formulation and production of food products.

19.1 Introduction

Food safety is one of the important factor for food industry, regulatory agencies and consumers. Nowadays consumers are demanding food which is safe and of high quality in terms of nutrition due to a healthier life style. Food safety refers to all those hazards, that make food injurious to the health of the consumer and it is not negotiable. Food may be contaminated physically, chemically or microbiologically, mainly microbial contamination is of high concern in the food industries. Various pathogenic and spoilage causing microorganisms, viruses, insects can deteriorate the food products and cause food borne illnesses. Therefore, there is a need to eliminate these undesirable microorganisms from the food industries to ensure food safety to the consumer. Various methods are available to eliminate these undesirable microorganisms such as thermal technologies like pasteurization, dehydration, retort processing, freezing etc. and non thermal technologies like microwave heating, Pulsed Electric Field (PEF), High Pressure Processing (HPP), Pulsed Light Technology, Ohmic Heating, Ultra sonics, Pulsed X-Rays. But these technologies have certain disadvantages like thermal technologies lowers the nutritional value and leads to unwanted changes in the foods' sensory attributes of the food products whereas non thermal technologies retain better nutritional and sensory quality of food than thermal technologies but they are cost effective, required specialised equipments and trained personnel. Cold plasma technology (CPT) is a non-thermal green technology used now-a-days for variety of application in various industries especially in food industry. It is proposed to be an alternative method for eliminating microorganism from the fresh produce. This technology does not require extreme process conditions and can be used as a good alternative source for food preservation without affecting its nutritional and sensory attributes. Plasma is an ionized gas, consisting of active species like positively and negatively charged ions, electrons, free radicals, and gas atoms, metastable and quanta of electromagnetic radiation (photons). When the energy source is turned off, active species used to disappear immediately, therefore cold plasma technology is environmental friendly and fulfilling all ecological standards. Based on the properties of plasma, it can be applied in various fields like electronics, life sciences, textile, packaging and food industries. In food industries, it is showing promising application by retaining the nutritional, functional, and sensory characteristics thus ensuring the fresh appearance of the food product and satisfying the consumer need.

Introduction

Food grains have been the key component of the human diet for thousands of years and have played a vital role in framing human civilization. Rice, wheat, and maize, and to a lesser extent, coarse grains are major staples, crucial to the daily endurance of billions of people around the world. Cereal grains are consumed by more than 50% of world population to meet daily calorie requirements. Most grains undergo a milling process that ideally removes the outer bran (pericarp) and germ layers; catering to consumer market requirements. To a farmer, yield is the most prominent aspect. As the grain moves along the supply chain, and as the product reaches the market, quality becomes the key determinant of its sale-ability and economic value. Grain quality is defined by a range of physical and compositional properties where threshold necessities are set according to end consumer use requirements. Most industries and consumer markets have become habituated over the years to demand grain with specific attributes. Where consumers are close to the source of grain production (e.g. in local markets), their own preferences and the laws of supply and demand control the grain quality. However, where grain is traded over large distances, particularly internationally, consumers almost have no direct influence on quality, requiring regulatory standards to be established and imposed to protect consumer rights. Therefore in the grain supply chain, all points have effects with respect to grain quality.

Postharvest handling is the stage of crop production immediately following harvest, including cooling, cleaning, sorting and packing. The instant a crop is removed from the ground, or separated from its parent plant, it begins to deteriorate. Postharvest activities include harvesting, handling, storage, processing, packaging, transportation and marketing. Losses of horticultural produce are a major problem in the postharvest chain. The diseases which develop on harvested parts of the plants like seeds, fruits and also on vegetables are the postharvested diseases. The amount or extent of damage depends mainly on the pathogen(s) involved, on the condition of the products and the condition of storage. Major tips to avoid postharvest losses include Tips to avoid postharvest losses include assessing maturity, to check your water quality, to check water temperature, avoid injury, keep the produce cool and proper storage.

“Storage” means the phase of the postharvest system during which the products are kept in such a way as to guarantee food security other than during periods of agricultural production at the marketing level, to balance the supply and demand of agricultural products, thereby stabilizing market prices. Grain, which includes dry kitchen ingredients such as flour, rice, millet, couscous, cornmeal, and so on, can be stored in rigid sealed containers to prevent moisture contamination or insect or rodent infestation. For kitchen use, glass containers are the most traditional method. Maturity indices are the sign or indication the readiness of the commodity for harvest. It is the basis for determining harvest date. Postharvest losses occur between harvest and the moment of human consumption. They include on-farm losses, such as when grain is threshed, winnowed, and dried, as well as losses along the chain during transportation, storage, and processing.

Introduction

Eradication of malnutrition and hunger (Sustainable Development Goal, SDG-2) are the major challenges to humanity. Availability of sufficient nutritionally balanced food is the prerequisite for ensuring global food security. Food security is also critical for achievement of SDG 1 (poverty eradication) and SDG 3 (health and well-being) (UNDP, 2019). Changing climatic conditions accompanied by global warming not only acts a major threat for achievement of SDGs but could actually lead to the reversal of the progress already made. The escalating global temperature has jeopardized the food security throughout the world. A great decrease in the global crop yield has been suspected even with a 2°C rise in temperature. On field, it is often associated with crop nutrient loss, less accumulation of micronutrients and low availability of micro-minerals like iron and zinc. Additionally, the effect of the rainfall and temperature has also been observed. The global food prices are projected to increase by 3–84% by 2050 due to the fluctuations in rainfall and temperature (Hoegh-Guldberg et al., 2018).

Millets are the small seeded grains which belong to family Grassacae/Poaceae. Traditionally, millets are used for the food and feed purpose and are among the oldest cultivated crops. The millet species of commercial importance are proso (Panicum miliaceum), foxtail (Setaria italica), barnyard (Echinochloa esculenta), finger (Eleusine coracana), little (Panicum sumatrense) and pearl (Pennisetum glaucum) in different regions of the world. In India, foxtail millet (priyangava), barnyard millet (aanava) and black finger millet (shyaamaka) have been mentioned even in the Yajurveda texts of 4500 BC (Morya et al., 2017). The records of consumption of foxtail and proso millet are as old as 2000 to 1000 BC in China. Cultivation of foxtail millet in Korean peninsula is documented around 3,500–2,000 BC. Foxtail millet is the oldest of all the cultivated millets and is believed to be originated in Southern Asia. From there, it was spread to Europian nations. In the west, cultivation of millets majorly started after 1850 (ICRISAT, 2019)

The method to preserve foods in sealed glass bottles by heat in boiling water was developed by one among the following scientist?

a) Edward Jenner (1798) b) Francois Nicolas Appert (1804)

c) Lazzaro spallanzani (1765) d) None of the above

Introduction

A biopolymer is defined as a polymeric substance obtained from any living organism (plant or animal) [1]. These polymers are organic compounds where simpler molecules are covalently bonded with each other [2]. Although biopolymers are strictly synthesized naturally by living cells but some of them are also synthesized from non-biomaterial as well. Natural biopolymers are of different types based on their chemical nature like carbohydrate based, protein based or lipid based. Biopolymers can be broken down by the action of naturally-occurring organisms which leaves organic non-harming by-products like water and carbon dioxide in the environment[3].

Bio polymers are gaining importance in the field of food packaging, medical application, commodity packaging etc. because of their have specific physical, chemical, biological, biomechanical and degradation properties. Wide ranges of natural or synthetic biopolymers capable of undergoing degradation hydrolytically or enzymatically are being investigated for many applications[1]. Bio polymers are mainly based on two properties: a) Biological origin b) Biodegradability.

Earlier natural biopolymers such as cellulose, protein, starch have been used extensively for food applications. Recently, synthetic biopolymers such as polylactic acid (PLA), polycaprolactone (PCL), polyglycolic acid (PGA), polyvinyl alcohol (PVOH), and polybutylene succinate (PBS) are gaining more attention[4] for various applications. Natural biopolymers are more stereospecific and mostly hydrophilic having complex 3D structures as compared to the synthetic polymers[2]. Among the synthetic biopolymers some are microbiologically degradable in the environment whereas some are recyclable[1].

    1.    Control points are the points in the food processing chain where it is possible to control or remove hazards.

    2.    Critical control points are the control points in the processing chain where it is essential to a hazard, usually because there is no later step at which to establish control.

    3.    Preventative measures are the actions and activities needed to remove hazards or control them by reducing them to acceptable levels.

    4.    Critical limits for critical control points are measurements such as temperature and time, that must be met, or characteristics such as appearance and texture. Critical limits need to be validated.

    5.    Monitoring is the regular measurement or observation of a critical control point to ensure it is not beyond its critical limits.

    6.    Take corrective action to bring the process back under control before the problem (‘deviation’) leads to a safety hazard. Consider proper management (disposition) of any adversely affected product.

    7.    Verification procedures are tests and programmes that make sure the HACCP system working properly

    8.    Record keeping and documentation systems must meet the needs of the business and be adequate to show that the food safety programme is working.

1. Introduction

Fruit products have their unique identities having large content of micronutrients, polyphenols, vitamins, flavonoids and other nutritional compounds. Characteristic appearance and flavors distinguish among various fruit based products like juice, puree, jam, jelly, squash and other several varieties. Main concern of this fruit processing industry is the stability of the products throughout the post processing storage. Thermal treatment as mean of preservation of these products is the common process implemented so far. It ensures the safety of the product but lacks in retaining the nutritional intact that like of fresh fruit. Today’s consumer demand for minimally processed fruit products and “natural” fruit products tend to think of alternative thermal processing.

High pressure processing (HPP), a relatively new concept compared to conventional thermal processing, also sometimes known as high hydrostatic pressure (HHP), or ultra high pressure (UHP) processing, is a non-thermal food processing method that subjects liquid or solid foods, with or without packaging, to pressures ranging between 50 and 1000 MPa. HPP has already been established itself as an alternative to thermal processing. In food processing it is of great interest due to its ability to inactivate food borne microorganisms and enzymes, at low temperature, without the need for chemical preservatives and it is less detrimental than thermal processes to low molecular weight food compounds such as flavoring agents, pigments, vitamins, etc., as covalent bonds are not affected by pressure. Pressure-treated foods have sensory properties similar to fresh products, which is a major advantage in fruit processing as it matches consumer demand for healthy, nutritious and ‘‘natural’’ products. HPP of fruit products offers the chance of producing food of high quality, greater safety and increased shelf life (Hayashi 1989; Gould 1996; Butz and Tauscher 1998).

1. Introduction

Storage is an important part of any food grain processing chain. Storage of raw and processed food grain has been used by humans since time immemorial as a prerequisite for insuring food security due to off time availability, and for holding seed grain for long periods (Pande and Mishra, 2012). After harvesting grains may be required to be stored for long periods before these are marketed or used as food or seed. The grain storage is mainly carried out for three purposes viz (i) to retain a supply of food (ii) to service a trading system and (iii) to retain seed for planting the following season (Hall, 1980). Storage is, therefore, carried out by the producer, trader, processor, and the exporter and at all these levels the methods adopted affect the quality of food stuffs. The length of time grains can be safely stored will depend on the condition it was harvested and the type of storage facility being utilized. Grain storage and handling is a major concern for food grain growers and processers worldwide (Mohan et al., 2011; Pande and Mishra, 2012). Depending upon the condition and uses food grains may be stored in different storage structures using traditional or modern storage methods. Different storage structures are used by farmers with the traditional names like Gumma, Oliya and Jute bag. Caswell (1973) recorded 50 to 60% of insect damage on food grain after six months of traditional storage. A typical range of storage methods encountered in tropics and subtropics region is given in Table 1.

1. Introduction

India is a land of large varieties of fruits and vegetables due to its vast soil and climatic diversity. India is the second largest producer of fruits and vegetables in the world, with an annual production of fruits 45.5 Million tonnes and vegetables 90.8 Million tonnes. It contributes 10.23% and 14.45% of the total world production of fruits and vegetables (F&V) respectively (NHB, 2010). Though fruits and vegetables are grown only on 7–8% of gross cultivated area, these contribute more than 18.8% of the gross value of agricultural output and 52% export earnings from total agricultural produce. These have a high export potential of 20-30 times more foreign exchange per unit area than cereals due to higher yields and higher price available in the international market (Sen et al., 2012). According to estimates, the per capita consumption of fruits and vegetables in India is only around 46g and 130g which are far below the stipulation of a minimum of 92g and 300g respectively as recommended by the Indian Council of Medical Research and National Institute of Nutrition. The low availability is mainly due to considerably high post-harvest losses. About 15-20% of the fruits and vegetables are wasted every year in the country, due to poor post-harvest management and lack of infrastructural facilities for processing and storage, amounting to a revenue loss of over Rs. 625 billion.

The sensorial, nutritional, and organoleptic quality of fresh produce starts deteriorating after harvest as a result of soon after harvest the altered plant metabolism and microbial growth. The quality deterioration is the result of produce respiration and transpiration, senescence, ripening-associated processes, wound-initiated reactions, development of postharvest disorders, microbial proliferation leading to postharvest quality loss. Therefore, it is necessary to understand the respiration and ripening processes and the physiological and biochemical changes during ripening. The best way to reduce respiratory metabolism is to reduce the storage temperature. All biological processes proceed more slowly at lower temperature (Yahia, 2007). In general, O₂ levels must be reduced to less than 10 % to see a reduction in the rate of respiration. Respiration rate is temperature dependent; so, as the temperature reduces the amount of oxygen required also drops. Therefore, oxygen levels must be substantially reduced at low temperature for it to affect the rate of respiration. It must however, be ensured that O₂ levels do not fall below extinction point as anaerobic respiration and decay may occur. Tolerance to low O₂ varies between species and cultivars and is also time dependent. Commodities may be able to tolerate low O₂ for short periods of time. Low O₂ can also be tolerated when CO₂ is low.

1. Introduction

Foods are complex biologically and chemically active systems that require close monitoring of their manufacturing, distribution and storage conditions in order to maintain their nutritive value, safety and sensory qualities by suitable chemical. The determination of trace impurities in food system presents considerable difficulties owing to complexity as it contains thousands of major and minor compounds (Nilufer and Boyacio, 2002). The choice of the method of analysis depends on the sample, the analyte to be assayed, accuracy, limit of detection, cost and time to complete the analysis (Aboul- Eneim et al., 2000). The chemical methods employed for food analysis and quality control are generally time consuming and require tedious or destructive sample preparation. For development of the new detection method, emphasis should be on development of simplified, cost-effective and efficient procedures that complies with the legislative requirements (Stroka and Anklem, 2002; Enker, 2003).

The molecular spectroscopy is a fast, accurate, easy and non-destructive technique that can be used as a replacement of time-consuming methods. Near infrared (NIR) spectroscopy has proved to be a powerful analytical tool used in the agricultural, nutritional, petrochemical, textile and pharmaceutical industries (McGlone and others, 2002; Esteban-Diez and others, 2004). Infra red spectroscopy is based on the absorption of infra red light by the substance to be measured. This absorption excites molecular vibrations and rotations, which have frequencies that are the same as those in the infrared range of the electromagnetic spectrum (Pande and Mishra, 2015). This application uses a fiber optic coupled FT-NIR system operating in the 10,000 – 4,000 cm^-1 region in reflectance mode. It involves the analysis and quantification of molecular responses to introduced signals of known energy or frequency. All molecules (e.g. water, glucose, protein) have a definite amount of energy. When alternate energy (e.g. infrared radiation) is introduced, an energy exchange occurs between the introduced energy and the energy contained within the molecule. This is expressed as absorbance (energy is absorbed resulting in a loss of introduced energy), attenuated (energy is scattered resulting in a loss of introduced energy) or emitted (energy is released resulting in a gain on the introduced energy). As individual molecules and molecular groups (e.g. alcohols, nucleic acids, proteins, sugars and fats) have definite energy, by applying external energy of a known amount/type, one can structurally identify, quantify and even determine the natural state of these molecules within complex samples and mixtures.

    1.    Asepsis is a process of 
        a)    Removal of microorganisms    
        b)    Keeping out microorganisms
        c)    Hindering the growth & activity of microorganisms    
        d)    Killing the microorganisms
    2.    Self-decomposition of the food is prevented by
        a)    Inactivation of enzymes    b)    Delay of chemical reactions
        c)    a & b    d)    None
    3.    Commercially available fruit beverages are generally pasteurized at 
        a)    85°C for 25 to  30 min    b)    62.5°C for 25 to  30 min
        c)    71.1°C for 15 to  30 sec    d)    92.1°C for 15 to  30 min

Abstract

Genomics is playing an increasing role in generating new tools and databases with the rapid advances in genome technology which increases the eficiency and precision of crop improvement. With the emergence of new high-throughput technologies like next-generation sequencing (NGS) and reduction in their costs are bringing many more plants within the range of genomic and post genomic data along with the integration of information from the related fields of transcriptomics, proteomics, metabolomics and phenomics. Translating the vast abundance data produced by genome technologies requires large generic databases which hold specific data types for a broad range of species, which act as a resource for the crop improvement. The development of custom bioinformatics tools and advanced databases has become an expanding challenge which systematically helps in storing, organizing, manipulating and analyzing large amounts of biological information computationally. In this review, we outline some recent advances in the features of plant genome databases and various platforms with the recent advances in technology.

1.    Evolutionary relationships between groups of organisms are determined using comparisons of

(a) Biochemical pathways

(b) Nucleotide sequences

(c) Structural features

(d) All of these

2.    The different bacterial species can be subdivided into

(a) Serovarieties

(b) Biovarietites

(c) Subspecies

(d) All of these

3.    Kingdom Prokaryotae include

(a) Bacteria

(b) Protozoa

(c) Fungi

(d) None of these

1. Size reduction is also referred as

(a) Extraction

(b) Communication

(c) Leaching

(d) Distillation

2. Size reduction causes

(a) Decrease in the temperature of the product

(b) Increase in temperature of the product

(c) Increase in pressure

(d) Decrease in surface area