eChapters

3.1. Origin and distribution

• Originated in the Southern Europe, Mediterranean region and grows throughout Northern, Eastern, and Western hemispheres specifically in Asia, Rumania, North America, Europe, India, Russia, Germany and France.

• In India, cultivated in Rajasthan, Andhra Pradesh, Punjab, Madhya Pradesh, Uttar Pradesh, Maharashtra, Gujarat, Karnataka, and Haryana.

3.2. Economic importance

• The leaf stalks are used both as vegetable and salad.

• A common practice in most Indian households is to have few fennel seeds at the end of every meal to freshen the mouth.

• Used for various digestive problems including heartburn, intestinal gas, bloating, loss of appetite and colic in infants.

• Source of copper, potassium, calcium, zinc, manganese, vitamin C, iron, selenium and magnesium.

• The seeds are used as condiment

• Seeds yield 4-5% volatile with anethole as major constituent.

1. Introduction

Extrusion of foods is an emerging technology for the food industries to process and market a large number of products of varying size, shape, texture and taste. Extrusion cooking of cereals is becoming more adaptive due to its various advantages over the traditional food processing techniques (White, 1994). Nowadays the extrusion of food is of major importance in the food-processing sector and it is the only process, which covers a wide range of food products such as ready to eat (RTE) breakfast cereals, snack foods, baby foods etc. Elaborate scientific research, technological development and engineering design, which have been done in this field so far, have led to large-scale industrial production. 

In spite of late introduction, different forms of chilli (Capsicum spp.) did spread throughout the length and breadth of India. Diverse soil and climatic conditions prevailing in different parts of Kerala have contributed to the development of different ecotypes in chilli. Warm humid climate in the coastal belts, dry and arid climate in the central eastern parts and mild subtropical to temperate climate in the high ranges provide ideal condition for development, maintenance and spread of different ecotypes in the crop. Plethora of chilli varieties capturing the markets of Kerala comprise of different species.

Capsicum chinense commonly known as Ney Mulaku, Karanampotti Mulaku is gaining popularity in the state due to increasing demand from Maldives during Ramzan period. Hence the crop is also known in yet another name Mali Mulaku. This species is characterized by perennial and spreading nature with medium sized oblong to round, fleshy and highly pungent fruits. It  yields substantially higher under shaded condition and there is less incidence of diseases like bacterial wilt and collar rot. Hence the species is ideal for homestead cultivation. Due to its tolerance to shade, the species can be successfully grown as an inter crop in coconut, rubber, pepper and banana plantations.

Cleome gynandra is one of the most underutilised crops in the world. It is the best known by rural and urban communities of Africa. The plant is well distributed in parts of South Africa, C. gynandra is indigenous to the country and evenly spread throughout Africa. Many people use it in their daily diets but it is not yet found on formal commercial markets. It is still regarded as a weed by commercial farmers. The nutritional value of the leaves is totally underestimated. The plant is known for the treatment of various ailments originated from epilepsy to headaches. The crop is harvested mainly from the wild and only cultivated in certain areas. The germination of the seeds can be a problem especially if the seeds are harvested simultaneously. The seeds must be harvested as it gets matured not all at once. Most consumers harvest the plant as needed. The more the leaves are picked the more growth is stimulated. A general harvesting method is not worked out yet, as this will differ from area to area.

Exacum bicolor, Roxb. commonly known as country kreat is  endemic to Peninsular India. It is a herbaceous perennial with attractive flowers and posseses medicinal properties. Plant is endangered today due to large scale habitat destruction.  Non- lodging nature of plant, attractive bi-colored flower bunches with a long flowering period and good field life offer great future for this plant to be domesticated as an attractive garden flower. Its ornamental value and medicinal properties are yet to be fully exploited.

India is bestowed with a wide array of flowering plants. It represents two of the 18 hotspots of biodiversity in the world; one being the Western ghats and other the North Eastern Himalayas. Nature has also blessed our India with  uniqueness of endemism. Of the 17000 flowering herbs distributed in nine phyto geographical regions of the country, 5700 are endemic. Exacum  bicolor Roxb. is one of the endemic species to Peninsular India. Plant is popularly known as Bharachirata and Habshi chirata in Hindi, Kannamthali in Malayalam, Dodda  chirayuta in Kannada and ceti in Tamil.

ABSTRACT

Phospholipase A₂ catalyzes the hydrolysis of glycerophospholipids at the sn-2 position and releases fatty acids and lysophospholipids. When the fatty acid is the arachidonic acid, a complementary metabolism leads to pro-inflammatory mediators such as prostaglandins, leukotrienes, thromboxanes and platelet activating factors. Thus, modulating pro-inflammatory lipid mediator production by inhibiting PLA₂ activity remains a potential target for the development of new drugs for the treatment of inflammatory diseases. Medicinal plants have been explored therapeutically in traditional medicines and are a valuable source for drug discovery. Molecular docking studies were carried out for anti-inflammatory compounds from various plant sources with human non-pancreatic secretory phospholipase A₂.

Genetic resources for food and agriculture are the biological basis of world food and nutrition security and they directly support the livelihood of people. Horticultural genetic resources are a few of biological assets available to resource-poor farmers to ensure sustainable production to improve livelihood options. Genetic diversity gives resource-poor farmers a low cost option to manage the shocks and vulnerability of production systems by its ability to adopt to changing environments. It is the foundation upon which plant breeding depends for the creation of new varieties and is, therefore, of critical public value for global food security. The rapid loss of genetic diversity (genetic erosion) is a worldwide concern and is particularly severe among crop landraces. Farmer’s varieties or landraces may contain co-adapted gene complexes that have evolved over decades and are the most valued genetic resources as they provide options for a range of climatic and edaphic stresses. Therefore, agricultural biodiversity has a critical public value for global food security (FAO, 1978).

ABSTRACT

The in vitro culture studies in crop plants show that there is a differential response of various explants to different concentrations of auxins and cytokinins. This indicate that hormonal requirement is specific to the genotype and developmental stage of the explant. Therefore standardisation of media and explants were done for in vitro callus culture and plant regeneration in L. esculentum and L. pimpinellifolium at the Department of Olericulture, College of Horticulture during 2000-2002.

The source materials selected for the study were L. esculentum var. Sakthi and L. pimpinellifolium. The explants were leaf segments (0.5 cm²) from fully opened leaves, nodal segments (0.5 cm) and internodal segments (1.0 cm). The media consisted of the basal MS and ½ MS media along with different auxins and cytokinins either singly or in combination. The auxins used were NAA (0, 1, 2 and 3 mg l^-1 and 2,4-D (0.5, 1, 1.5 and 2 mg l^-1) and the cytokinins used were BA and kinetin (1, 2, 3, 4 and 5 mg l^-1).

ABSTRACT

Advances in biotechnology, particularly methods for culturing plant cells and tissues, can provide new means for the commercial processing of even rare plants and the chemicals they produce. These new technologies will extend and enhance the usefulness of plants as renewable resources of valuable chemicals. Aristolochic acid is mainly found in the genus Aristolochia. Aristolochic acid has been shown to have anti-tumour activity and has been recommended for its activity against various effects of snakebite and its capacity to inhibit phospholipase A₂ or other enzymes. Considering the importance of medicinal use of A. bracteolata and A. indica, in the present study, they were selected for in vitro development and rapid multiplication of plant through tissue culture. The study was undertaken to enhance the in vitro production of Aristolochic acid in different tissue cultures. Cell suspension culture was established from friable calli of both the species in MS liquid medium. Bergmann plating technique was carried out for cell line selection for more production of Aristolochic acid. Primary transformation was carried out by Agrobacterium tumefaciens strain LBA4404 / pTOK233 with Gus and hpt genes to confirm the transgenic potential of A. bracteolata and A. indica. In order to enhance the production of aristolochic acid in in vitro culture condition, the physical, chemical and biological conditions were altered. Aristolochic acid was isolated, identified and purified from naturally growing plants as well as from tissue culture plants and all other culture staes

ABSTRACT

Dendrophthoe falcata (L.f.) Ettingsh, a unique hemiparasitic taxa belongs to the family Loranthaceae, spreads over the entire forest in the South West Bengal. It is generally stem hemiparasitic taxa, as it is found mostly on aerial part of the host plant due to lack of root system. It depend on the host plant for mainly water and to some extend carbon. It synthesizes its own food. This plant has haustoria instead of root, they penetrate their haustoria into the vascular bundle mainly in the xylem tissue of the host plants. This species has numerous medicinal values with a long history of its use in Chinese traditional medicines. The present work deals with the anti-diabetic, phytochemicals and silver nanoparticles properties of D. falcata. The responsible enzyme i.e. Alpha-amylase breaks the large starch molecules which produces free glucose and simultaneously increases the blood sugar level and as a result hyperglycemia occurs. This study also reveals that the plant’s leaves extracts have inhibitory activities on this key alpha amylase enzyme and apart from that aqueous extract of this plant contains some secondary metabolites which is very crucial data for drug preparation and any other research purposes. The maximum amount of silver nanoparticles is present in this plant which is valuable as a silver line in near future implications for different drugs preparations

26.1 Introduction

We have already discussed the basics of tissue culture techniques in Chapter 2. Plant tissue culture techniques in combination with recombinant-DNA technology are essential requirements for development of transgenic plants (will be discussed in next chapter). However, tissue culture techniques like anther, pollen, ovule and meristem culture can themselves be used for improvement of different traits or as an aid to conventional breeding methods. In recent years, isolated microspore culture has been preferred as a breeding tool and an experiment system for various genetic manipulations. Besides, using protoplast fusion and hybrid embryo rescue, distant hybridization has been successfully achieved in many crops. Protoplast fusion technique can be used for the transfer of cytoplasmic male sterility from one species to another in very short duration. Somaclonal variation generated during tissue culture can also be utilized for recovery of variants showing disease resistance or other desirable characters.

We present in this paper the data about the popular uses of elder (Sambucus nigra) obtained in ethnobotanical studies carried out in the districts (“comarques”) of Pallars Jussà, Pallars Sobirà, Cerdanya, Conflent, Capcir, Ripollès and Alt Empordà (Pyrenees) and in the Montseny massif (Catalan Prelittoral Range), complemented with others from different Catalan regions (also investigated by our group), and compared with those from other Iberian and Mediterranean territories. This bush or small tree is one of the most reported and used plants (for medicinal and other purposes), not only in the regions studied, but in all Catalonia and in many other areas as well. It is one of the most versatile plants, since its uses embrace many different fields like medicinal, food, ornamental, craft industry and games among others. It is, in addition, aremarkable fact that almost every part of the plant (bark, root, leaves, flowers, fruits) has some uses.

The genus Sambucus L. is widely distributed in temperate and subtropical zones (Mabberley 1990). According to a few authors, it constitutes about 20-25 species (Mabberley 1990, Bolòs and Vigo 1995), whereas a more synthetic treatment (Bolli 1994) considers only nine species with 10 subspecies. The only three European species of this genus (Ferguson 1976) are S. ebulus L.

Mature seed samples of Vigna dalzelliana (O.Kuntze) Verdc. were analysed for proximate composition, total (true) seed protein fractions, amino acid composition, fatty acids profiles, mineral and antinutritional factors. The investigated seed samples contained higher amount of crude protein, crude fat, ash and nitrogen free extractives and constitute 27.33,7.48,6.71 and 50.12g/100g. seed powder respectively. The calorific value of seed material was 377.12  K.cal / 100 g Dm. The essential amino acids, isoleucine, tyrosine and phenylalanine, were present in relatively large quantities. The fatty acid profiles revealed that the seed lipids contained higher concentrations of palmitic and linoleic acids. The seeds are rich in sodium, phosphorous, calcium, zinc, manganese and iron. Anti-nutritional factors, such as total free phenols (8.26%), tannins (6.1875 %), L-DOPA (0.96%), hydrogen cyanide (1.94 %) and phytic acid (7.32%) are present in variable quantities. This paper presents the analytical data on the chemical composition and nutritional potential of Vigna dalzelliana seeds with regard to its suitability as a regular component in human diet.

1.    Groups of Apomixis
    
2.    Recurrent Apomixis
    
3.    Non-recurrent Apomixis
    
4.    Vegetative Apomixis
    
5.    Genetic Control of Apomixis
    
6.    Mechanism of Apomixis
    
7.    Ovule, Seed and Fruit Development in Sexual Plants
    
8.    Double Fertilization and Control of Embryo Initiation
    
9.    Sequential Events in Apomictic Pathway
    
10.    Gametophytic Apomixis
    
11.    Significance and Scope of Apomixis in Crop Plants
        
References

Abstract

The term nano technology, buzzword of present day science owes its origin from the Greek word ‘nano’ literally meaning dwarf. It is expressed in terms of dimension one nanometer equals to one billionth of a meter (1nm=10-9m), work on the principle of particle movement, smallest particle accelerate fast in the system. The nano technology deals with manufacturing, study and manipulation of matter at nano-scale (or atomic scale) in the size range of  1-100 nm, called as nanoparticles (Rajan, 2004). Nano technology is one of the most fascinating and rapidly possessing potential to revolutionize in science and technology of medicine and agriculture. In plant pathology, nano technology has potential scope to detection, diagnosis and management of plant diseases. Micromechanical cantilever arrays for selective immobilization and fast quantitative detection of vital fungal spores (Nugaeva et al., 2005). Micro-fabricated chambers designed to mimic some of the features of xylem vessels and discovered that Xylella fastidiosa migrates via type IV pilus-mediated twitching motility (Meg et al., 2005). Antifungal properties of chitosan, chitosan-saponin and Cu-chitosan nanoparticle reveal against phytopatogenic fungi namely Alternaria alternate, Macrophomina phaseolina and Rhizoctonia solani (Sharan et al. 2013). 

Abstract

Beekeeping is an agro-based activity which is being undertaken by farmers/landless labours in rural area as an integrated framing practice. It supplements income & employment generation and nutritional intake. Honeybees have been offering services to the society through ensured pollination in crops and providing honey and a variety of other bee hive products viz.; royal jelly, pollen, propolis, bee wax, bee venom, etc. Honey Bees have their vital role in sustaining plants bio-diversity resulting in environmental stability. Mainly five species of honeybees viz.; Apis cerana, A. mellifera, A. florea, A. dorsata, Stingless bees are in used in India.  Beekeeping industry has quadruple benefits: 1) providing self employment to rural and forest based population; 2) production of honey, pollen and beeswax, venom, royal jelly, etc., 3) providing employment to rural educated youths in collecting, processing and marketing of bee/beehive-products; and 4) cross-pollination of various agricultural and horticultural crops and improving their quality and increasing yields.

1.    Biological Control of Insect Pests
    
2.    Biocontrol Agents in Vegetables
    
3.    Mass Multiplication of Predators and Parasites
    
4.    Microbial Control
    
5.    Transgenic Plants
    
6.    Biological Control of Diseases
    
7.    Conclusion
        
References

Introduction

Agriculture is one of the promising sectors which offers marvelous potential for sustainable growth and play a key role in the economy of the country. Many of the foods that are already common in our diet are obtained from plant varieties that were developed using conventional genetic techniques of breeding and selection. Increase in the world population and limited food supply strained researchers to introduce and advance agriculture management and modern technologies along with conventional practices to attain highest possible crop productivity. Agricultural technology is based on the domestication of wild plants to create the crops that we have come to depend upon. Humans invented agriculture approximately 10,000 years ago when they began to harvest and cultivate specific plants to produce food. The improved plant traits selected by early agriculturalists were transmitted genetically to succeeding generations of plants. Both public institutions and private companies are now involved in crop genetic improvement.

Abstract

Abiotic stresses have been a serious challenge for sustainable agriculture particularly vegetable cultivation characterized by decreased endurance against stresses like drought, temperature fluctuation and salinity. In India, drought and salinity being major abiotic stresses affect an area of 16% and 4.28%, respectively. Plant Breeders have to face many impediments while developing stress tolerant varieties. Developing tolerant varieties is quite difficult as tolerance varies with the developmental stages and has complex inheritance. This can be understood well by studying ontogeny of plants. Drought varies in time of occurrence, severity and duration. Field screening for tolerance is very difficult because of various interactions like drought × high temperature, high temperature × salinity interactions etc. Artificial screening in different stresses could be an alternative approach. The varieties developed with higher inputs at research stations fail to perform at farmers’ fields due to high genotype × environment interactions. Cultivated species have less genetic variability for abiotic stress tolerance but tolerance can be enhanced by using diverse and heterogeneous population. Though wild species possess genes for tolerance but these genes are closely linked with the undesirable gene(s) e.g. in tomato where S. cheesmanii, S. peruvianum, S. pimpinellifolium and S. sitiens have undesirable traits which affect yield and quality adversely.

Abstract

Modern agriculture has disturbed the age-old balance of host-pathogen system resulting diseases occur at regular interval leading to enormous loss to the agricultural production, which is estimated to be 10-25% or less during normal years but may extend up to 100 % during epidemic. Broadly, there are two breeding approaches adopted for disease resistance which includes conventional and molecular one. Conventional approach includes pureline, pedigree, backcross, recurrent selection and interspecific hybrids through which various resistant varieties has been developed. However, after sometime there is sudden outbreak of diseases due to continuous sowing of these varieties which results in the advancement in molecular biology and with the discovery of trait linked markers it paved a new pathway to look a new corner to develop varieties which are disease resistant. Molecular approach consists of gene pyramiding through which two or more genes providing resistance to different types of diseases is introgressed in a particular variety, gene deployment and multiline varieties which consists of heterogeneous population formed by mixing the cultivars to differ in the ability of disease resistance and reduces the rate of epidemic development. With the help of makers in marker assisted breeding using foreground, recombinant and background selection several linked markers are identified which provides resistance against disease like in wheat stem rust, leaf rust etc.

Introduction

High density planting in fruit trees was first introduced in case of apple in Europe during the nineteen sixties, with the development of Malling and Malling - Merton rootstocks. Subsequently, this was experimented in other temperate fruit crops like peach, plum, pear and sweet cherry. Today, majority of new orchards of apple and to some extent that of other fruit crops in Europe, New Zealand, USA and Australia use this intensive system of fruit production profitably Iyer and Kurian (2006). The successful management of trees in any high-density planting system depends on maintaining a balance between vegetative growth and fruiting.

Canopy management is the manipulation of tree canopies to optimize the production of quality and quantity fruits. The canopy management, particularly its components like tree training and pruning, affects the quantity of sunlight intercepted by trees, as tree shape determines incoming radiation. Light is critical for growth and development of trees and their fruits. The green leaves harvest the sunlight to produce carbohydrates and sugars which are transported to the sites where they are needed – buds, flowers and fruits. Better light penetration into the tree canopy improves tree growth, productivity, yield and fruit quality. The density and orientation of planting also impact light penetration in an orchard (Proietti et al., 2000). Generally, in close planting, quicker shading becomes a problem. An east-west row orientation results in more shading as compared to the western and southern orientation of trees. The problem of a fruit grower is initially to build up a strong and balanced framework of the trees, then equips them with appropriate fruiting.

1.    Classification of Trees
    
2.    Flowering and Foliage Trees of Indian Gardens
    
3.    Palms and Cycads
    
4.    Coniferous Trees and Deciduous Trees for Temperate Climatic Situations
    
5.    Propagation and Planting of Trees
    
6.    After Care of Trees
        
    Further Reading

Abstract

Mango (Mangifera indica L.) is the one of the most important fruit crop of tropics and subtropics. Mango belong to group of plant in which there is an antagonism between vegetative vigour and flowering intensity. Therefore, any factor that reduces vegetative vigour without altering the metabolic activity and favors flowering which can be possible by the use of growth regulator. Growth retardant that has been widely used in mango crop is paclobutrazol (PBZ) or cultar. Paclobutrazol is N-containing heterocyclic, trizole compound which stimulate flowering by inhibiting gibberellic acid pathway by inhibiting oxidation of ent-kaurene to ent-kaurenoic acid by blocking cytochrome P450 dependent monooxygenase enzyme. It is applied at the rate 2-5g/m of canopy diameter 3-4 months prior to desired date of flowering either by foliar spray, soil drench or trunk application. However, soil application is found more efficient on mango quality production than other. Paclobutrazol has pleiotropic response on mango cultivars. It has been found that application of paclobutrazol is effective in enhancing the protein mobilisation, starch, carotenoid, lycopene, phenol and cytokinin, abscisic acid and enzyme content in treated plant that induce flowering. It has been found that paclobutrazol is capable to reduce mango malformation and make plant tolerant in stress condition.

Introduction

Crop regulation is also known as flowering/ fruiting cycle management. It can also be termed as management of vegetative-reproductive balance. Some of the fruit crops bloom throughout the year without any resting period and produces two or three crops (bahar) in a year but yield and quality is not so good in all crop harvest. It is very essential to understand the flowering and fruiting behavior of crops and which bahar will give good crop with considering all the factors associated with a particular bahar. It is important for the available time to be partitioned to give an appropriate balance between the making of sources of resources which capture and utilized resources efficiently in generating harvestable yield and the filling of sinks for resources; and sink must mature in desired time for harvest. Apart from their importance in the determination of crop yield

1.    Scab
        
2.    Powdery mildew
        
3.    Fire blight
        
4.    Soft rot
        
5.    Bitter rot

1.    Panama wilt
        
2.    Moko or Bacterial wilt
        
3.    Sigatoka disease
        
4.    Anthracnose
        
5.    Bunchy Top

1.    Anthracnose
        
2.    Bean rust
        
3.    Yellow mosaic
        
4.    Common bean mosaic/green mosaic

1.    Foot rot or Leaf rot or wilt
        
2.    Anthracnose
        
3.    Bacterial leaf spot or stem rot

1.    Yellow vein mosaic
        
2.    Cercospora leaf spot
        
3.    Fusarium wilt
        
4.    Powdery mildew

1.    Damping off
        
2.    Phomopsis fruit rot or blight
        
3.    Collar rot
        
4.    Bacterial Wilt
        
5.    Little leaf

1.    Damping off
        
2.    Fruit rot or Die back
        
3.    Powdery mildew
        
4.    Bacterial leaf spot
        
5.    Cercospora leaf spot
        
6.    Fusarium wilt
        
7.    Viral diseases

1.    Wilt
        
2.    Rust
        
3.    Septoria leaf spot
        
4.    Powdery mildew
        
5.    Chrysanthemum stunt

1.    Gummosis
        
2.    Canker
        
3.    Scab/Verucosis
        
4.    Tristeza or quick decline
        
5.    Greening
        
6.    Exocortis or scaly butt

1.    Bud rot
        
2.    Stem bleeding
        
3.    Grey blight
        
4.    Basal Stem Rot

1.    Coffee rust/Oriental leaf disease
        
2.    Black rot or Koleroga
        
3.    Die back or Anthracnose
        
4.    Damping off/Collar rot
        
5.    Berry blotch

1.    Wilt
        
2.    Stem rot
        
3.    Alternaria leaf spot

1.    Club root or Finger and toe
        
2.    Downy mildew
        
3.    Powdery mildew
        
4.    Alternaria leaf spot
        
5.    White rust
        
6.    Black rot
        
7.    Bacterial soft rot

1.    Downy mildew
        
2.    Powdery mildew
        
3.    Anthracnose
        
4.    Cercospora leaf spot
        
5.    Alternaria blight
        
6.    Bacterial wilt

1.    Rhizome rot/soft rot
        
2.    Leaf spot
        
3.    Yellows disease
        
4.    Banded leaf blight
        
5.    Cercoseptoria leaf spot
        
6.    Bacterial wilt

1.    Downy mildew
        
2.    Powdery mildew
        
3.    Anthracnose/Bird’s eye spot

1.    Wilt
        
2.    Anthracnose
        
3.    Canker

1.    Rust
        
2.    Cercospora leaf spot
        
3.    Collar rot and Root rot
        
4.    Phyllody

1.    Anthracnose
    
2.    Powdery mildew
        
3.    Mango malformation
        
4.    Red rust
        
5.    Grey blight
        
6.    Sooty mould
        
7.    Stem end rot
        
8.    Bacterial leaf spot

1.    Damping off
        
2.    Purple blotch
        
3.    Smut
        
4.    Downy mildew
        
5.    Leaf blight (blast)
        
6.    Black mould

1.    Stem rot/Foot rot
        
2.    Powdery mildew
        
3.    Anthracnose
        
4.    Ring spot
        
5.    Leaf curl

1.    Downy mildew
        
2.    Powdery mildew
        
3.    Rust
        
4.    Wilt

1.    Leaf spot or blight
        
2.    Cercospora leaf & fruit spot
        
3.    Alternaria fruit spot
        
4.    Bacterial leaf spot

1.    Late blight
        
2.    Early blight
        
3.    Black scurf
        
4.    Common scab
        
5.    Bacterial Brown rot
        
6.    Wart
        
7.    Black leg (Soft rot)
        
8.    Viral diseases
        
9.    Potato spindle tuber

1.    Powdery mildew
        
2.    Die-back
        
3.    Black spot
        
4.    Rust