eChapters

Operculina turpethum (OT) Silva Manso, also known as Tihudi/Trivrit due to its triangular stem, is an Ayurvedic herb from India that belongs to the Convolvulaceae family. Indian Jalap/Turpeth root in English, Nisoth/Turpeth/ Panila/Pithori in Hindi, Trivrit in Sanskrit, Tegada in Telugu, Trikolpakkonna/ Triputa/Sivata in Malayalam, Sigade in Kannada, and Kumbham/Sivatai in Tamil are some of the other names for it. It grows up to 1000 square feet on Indian roadsides and is occasionally cultivated as an adornment in gardens.

Morphological characteristics

The crop is a fragrant perennial creeper with a basic stem that might be triangular or rectangular. The leaves are many and oval in shape, measuring 2 to 5 inches in length. It consists of cylindrical pieces of root and stem, 1.5-15 cm long x 1-5 cm diameter, with the central woody portion removed by splitting the bark on one side, external surface longitudinally furrowed giving the drug a rope-like appearance, odour distinct but unpleasant or musty, taste bland at first, then slightly acrid, fracture short in bark and fibrous in wood, odour distinct but unpleasant or musty. The root portions are cylindrical, twisted, and a dull grey tint on the outside. The flower arrangement is 1 to 4 inches long and includes three to four branches with white blooms. Fruit is round ½ or ¾ inch in diameter, it bears 4 shiny 2 inch long seeds. The plant bears fruits and flowers from March to December.

Pistacia integerrima Stewart (Pistaceaceae) is an ethnobotanically important tree native to Asia. Pistacia integerrima is a species of pistachio tree native to Asia, commonly called zebrawood or the crab’s claw. It is often classified as Pistacia chinensis ssp. integerrima.

Pistacia integerrima, commonly known as kakra shingi, is a moderate-sized deciduous tree, up to 18 m in height, with a short stout bole found in the Himalayas from Indus to Kumaon. Hard, horn-shaped, rugose, hollow galls like excrescences are formed on the leaves and petioles of the plant by an insect of Pemphigus species. Dry powdered galls have a very astringent and slightly bitter taste and terebinthine odour. This tree is also used as a rootstock in the cultivation of commercial pistachios

Morphological characteristics

It is a moderate sized deciduous tree with rough grey bark. Leaves are 15-23 cm long with or without a terminal leaflet. Leaflet is stalked, 4-5 sub-opposite pairs, lanceolate, coriaceous, entire and arched. Flowers are greenish-yellow or brownish in colour, dioecious, 0.2 cm diameter, reddish in lateral, puberulous panicles, appearing with or just before the young leaves.

.1. Origin and distribution

• Originated from West Africa.

• Cultivated in Malaysia, Indonesia, USA, Cameroon, Cote d’Ivoire, Ghana, Liberia, Nigeria, Sierra Leone, Togo, Angola and Congo.

• Commercial scale plantation in Malaysia was found in 1917.

9.2. Economic importance

• Produces edible palm oil as well as palm kernel oil.

• Maj or constituent of oil is mono unsaturated oleic acid; rich in carotenoids.

• Unrefined palm oil is a significant source of tocotrienol, part of vitamin E.

• Oil content is 17 to 27% for palm oil, and 4 to 10% for palm kernels.

Introduction

At the Banana Research Station, Kannara activities focused on Musa germplasm conservation and evaluation are in progress. The aim of the project is to collect and conserve wild and cultivated germplasm representative of the diversity existing in the Musa genepool for evaluation and subsequent use in breeding programmes and to make available selected superior material to the farmers of Kerala. The center presently holds a Musa germplasm of 256 accessions in its field gene bank. These accessions have been characterized and evaluated as per INIBAP/IPGRI Musa descriptors and a database developed.

In a vegetatively propagated crop like banana, in vitro culture is widely employed in germplasm introduction and conservation and is an essential requirement for the safe movement of germplasm (Sharrock and Engels, 1996). By this method, germplasm material is readily propagated, thereby facilitating the dissemination of disease free plants. Since 2001, efforts have been made at Banana Research Station, Kannara to introduce forty new exotic accessions employing in vitro culture for conservation and evaluation. These are material from the global Musa germplasm collection maintained by International Network for the Improvement of Banana and Plantain (INIBAP), France and supplied by National Bureau of Plant Genetic Resources (NBPGR), New Delhi.

Morphological characteristics

Straight bole, longitudinally fissured bark, imparipinnate and elliptic leaves, fragrant flowers in huge panicles, and winged, flat pods distinguish Pterocarpus species in the field. The tree can grow up to 30 metres tall and 2.5 metres wide, with a straight and clean bole. The bark is scaly, tough, and fissured longitudinally. Leaflets are five to seven in number, 8–13 cm long, oblong or elliptic in shape, or rotund in shape, with 15–20 pairs of lateral veins. Kino-gum is a fragrant, brittle, almost black oleo-resin obtained from tree trunks that is angular and glistering and comes in minute flakes..

Floral characteristics

Yellow flowers bloom in panicles that are 1–5 cm long and fragrant. Flat, orbicular, winged pods with a diameter of up to 5 cm. Seeds range from one to three in number, are bony, and have a convex form. Flowering starts in November, and fruits lasts until March.

Distribution

The tree can be found in central and peninsular India, primarily in dry mixed deciduous tropical forests in Gujarat and Madhya Pradesh, as well as sub-Himalayan tracts, at elevations of up to 1000 metres. Natural populations have plummeted, and there are hardly any fragile young saplings in the forest. Due to a lack of autogenic reproduction, this species is endangered.

10.1. Origin and distribution

• Native to the tropical Africa and grows in the Nepal, India, Bangladesh, Sri Lanka, Cambodia, Laos, Myanmar, Thailand, Vietnam, Malaysia, Indonesia and the Philippines.

• Palm is widespread in Tamil Nadu, Kerala, Andhra Pradesh, Telangana, Odisha, West Bengal, Bihar, Jharkhand, Karnataka and Maharashtra.

• In Tamil Nadu, it is present in Tuticorin, Tirunelveli, Thenkasi, Virudhunagar, Ramanathapuram Madurai, Theni, Salem, Namakkal, Kancheepuram, Chengalpattu, Tiruvallur, Villupuram, Kallakurichi and Cuddalore districts.

• Palm is dioecious in nature.

• Palmyrah was declared as “State Tree of Tamil Nadu” by the Government of Tamil Nadu.

Recent Initiatives by National Research Centre on Seed Spices, Ajmer

Standardization of the drying techniques

In fennel five methods of grain drying were employed viz., shade dryer (30°C), sun dryer (35°C to 37°C), solar dryer (40°Cto 42°C), vegetable dryer (50°C) and multipurpose grain dryer (60°C). Fennel grains which were dried under solar dryer, gave maximum test weight (5.17 g), recovery (39.82 %) with lowest moisture content (61.30%) and highest taste marks (9.3) and colour marks (9.2).

Morphological characteristics

Rewand chini is a perennial stout herb, 1.5–3.0 m tall. Roots are very stout and thick. Leaves are radical, orbicular, or broadly ovate, very large, and 30–45 cm in diameter with long petioles.

Floral characteristics

Flowers are small, dark purple or pale red in axillary panicles. The plant has three to five years of juvenile phase, followed by reproductive phase. Flowering occurs in June–July. Seeds are collected in late August–September, when they turn dark brown. Seed production potential varies from 300 seeds to 950 seeds per plant.

Distribution

The species occurs in the temperate Himalayas, from Kashmir to Sikkim, at an elevation of 2800–4000 m. It is found in alpine zone on rocky soil, moraines, and crevices, between boulders and near streams in specific pockets. It is sporadically cultivated in the North-eastern states for its leaves, which are used as vegetable.

Climate and soil

Rewand chini plant is restricted to the temperate, sub-alpine, and alpine zones of the Himalayas. Well-drained, porous, humus-rich soil is suitable for its cultivation. It prefers exposed or partially shaded habitat and can be cultivated on altitudes above 1800 m in height.

In the coffee family Rubiaceae, Rubia cordifolia L. (RC) is a useful medicinal plant in the Ayurvedic system due to its many pharmacological characteristics. It is also known as common Madder or Indian Madder. It was grown for a red pigment generated from the roots. Rubia was divided into roughly 70 species that were found all over the world, including 36 species and two variants described from China. Because of their high bioactivities, Rubia plant extracts and phytochemicals have gotten a lot of interest.

Morphological characteristics

It is a perennial, prickly climber with a stem, growing up to 12 m long. Leaves are highly variable, ovate lanceolate, 5-7 nerved, 2-10 cm long and 2-5 cm broad, occurring in whorls of 4-6. The stems are slender and quadrangular. The leaves are simple, opposite and stipulate, the stipule is interpetiolar. The petiole is 1.5–3 cm long. The blade is lanceolate, 1.5–5 cm × 0.5–2.5 cm, cordate at base, obtuse acuminate at apex and displays three to five longitudinal nerves. Roots are perennial, long, cylindrical, and rusty brown in colour

Morphological characteristics

Tecomella undulata is a slow growing small deciduous tree with drooping branches and grey-tomentose innovations which are otherwise glabrous. Leaves are usually opposite, 5- 10 cm long, simple, narrowly oblong, obtuse and entire with undulate margins.

Floral characteristics

Flowers are pale-yellow to deep orange, arranged in few flowered corymbose racemes on short lateral branches. Calyx is campanulate, lobes broadly ovate, obtuse and mucronate. Corolla is companulate, veined, stamens exserted, flaments glabrous, stigma 2-lamillate. Capsules are slightly curved, smooth and seeds are winged.

Distribution

Plant is distributed in drier parts of North-West and Western India extending eastwards to the river Yamuna and ascending to an altitude of 1200 meter in the outer Himalaya.

Climate and Soil The plant prefers subtropical climate having medium to light soil.

Environmental agents (xenobiotics) that are injurious to cells trigger a spectrum of responses that can range from cell death to adaptation, repair, and proliferation. Cell death, in turn, can take the form of apoptosis (a programmed form of cell death). Xenobiotics have been implicated in toxic cell injury, among them free radicals (nitrogen, oxygen, and carbon derivatives) are known to interact with cellular constituents including membrane lipids and chromagen materials.

Cytotoxicity

Cytotoxic: Toxic to cells, cell-toxic, cell-killing. Any agent or process that kills cells. The prefix cyto- denotes a cell. It comes from the Greek “kytos” meaning hollow, as a cell or container. Toxic is from the Greek “toxikon” = arrow poison.

In nutshell, cell cytotoxicity refers to the ability of certain chemicals or mediator cells to destroy living cells. By using a cytotoxic compound, healthy living cells can either be induced to undergo necrosis (accidental cell death) or apoptosis (programmed cell death).

Measuring cytotoxicity can prove to be very valuable tool in identifying compounds that might pose certain health risks in humans and animals. Cytotoxicity assays are widely used by pharmaceutical industry to screen for cytotoxicity in compound libraries from initial high-throughput drug screens for unwanted cytotoxicity. This can be of vital importance and prove to be quite indispensable during research phase of developing therapeutic new pharmaceutical products to ensure the safety of end-users. Additionally, understanding the mechanisms involved in cytotoxicity can likewise give researchers a more in-depth knowledge on the biological processes (both normal and abnormal) governing cell growth, cell proliferation and death. Compounds that have cytotoxic effects often compromise cell membrane integrity. Therefore, assessing cell membrane integrity is one of the most common ways to measure cell viability and cytotoxic effects. There are three commonly used methods which can be employed to assess in vitro cytotoxicity (disruption of cell integrity) in cell cultures:

Safety is a prime concern for humans and animals. Without evaluating the safety of a chemical, no regulatory authority in the world allows the chemical to be used by humans. There are stipulated guidelines for toxicity evaluations. Among the toxicity assays genotoxicity assays are important and have occupied centre stage due to increase in understanding of the genome.

Genotoxicity describes the property of chemical agents to damage the genetic information within a cell to cause mutations, which may even lead to cancer. While genotoxicity is often confused with mutagenicity, all mutagens are genotoxic; however, not all genotoxic substances are mutagenic. Generally and depending on the agent, a cytotoxic agent may be genotoxic (causing DNA damage), mutagenic (causing gene mutation) or it may be harmful for cell organelles (e.g. cell membrane) or it may even be teratogenic. Therefore, every cytotoxic agent is not genotoxic.

Why we Need to Evaluate Genotoxicity?

Toxicity can be at any level according to the nature of chemicals and type of exposure. Duration of toxicity can also be short lived or long lived within a generation or continue to next generation. Genotoxicity effects are able to continue in next generation(s), in which the exposed population may not suffer seriously but the next generation may suffer without being exposed to the same chemicals. Therefore, genotoxicity evaluations need serious attention.

Violaceae

Morphological characteristics

Banafsha is a small perennial herb with a short semi-subterranean fleshy stem (rootstock).The older parts bear fibrous roots. The upper younger portion is marked with close circular scars of the leaves of the previous years, and gives off elongated, rather fleshy, leafless runners. Leaves are ovate and crenate–serrate.

Floral characteristics

Flowers are borne on long, slender, axillary stalks (scapes) exceeding the leaves. Flowers are lilac, blue, white or purple in colour and borne on the main stem, the larger ones being 6–12 mm in diameter. The flowers arising from the axils of the cauline leaves are minute. The cleistogamous (underground) flowers measure about 1 mm across. Fruit is a few seeded, globose capsule. Flowering occurs during February–March, while fruiting occurs during April–May.

The concept of single cell micro-gel electrophoresis (SCGE)/comet assay is f irst introduced by Ostling and Johanson (1984) as a method to measure DNA single-strand breaks under neutral conditions. Singh et al. (1988) modified this technique by using alkaline conditions (>13 pH) of electrophoresis, which enabled detection of not only frank strands breaks but also alkali labile sites, DNA cross- linking and incomplete excision repair sites. Now-a-days, this technique is extensively used to study the genotoxic effects of various chemicals. The advantages of SCGE technique include: (1) collection of data at the level of individual cell, allowing for more robust types of statistical analysis; (2) need for small number of cells per sample (<10,000); (3) its sensitivity for detecting DNA damage; and (4) that virtually any eukaryotic cell population is amenable to analysis. The idea is to combine DNA gel electrophoresis with fluorescence microscopy to visualize migration of DNA strands from individual agarose-embedded cells. If the negatively charged DNA contained breaks, DNA supercoils are relaxed and broken ends are able to migrate toward the anode during a brief electrophoresis. If the DNA is undamaged, the lack of free ends and large size of the fragments prevented migration. Determination of the relative amount of DNA that migrated provided a simple way to measure the number of DNA breaks in an individual cell.

Genotoxic effects of any xenobiotics in PMNC and splenocytes of Wistar rats can be studied according to the working protocol of Dhawan et al.

Terminal deoxynucleotidyl transferase (dUTP) nick end labeling (TUNEL) is a method for detecting DNA fragmentation by labeling the terminal end of nucleic acids. TUNEL is a common method for detecting DNA fragmentation that results from apoptotic signaling cascades. The assay relies on the presence of nicks in DNA which can be identified by terminal deoxynucleotidyl transferase or TdT, an enzyme that will catalyze the addition of dUTPs that are secondarily labeled with a marker. Here we perform the apoptotic assay test by TUNEL assay kit to identify the apoptotic property of any agent or chemical after treatment of primary cells culture at different concentration as per protocol provided with TUNEL Assay Kit and apoptotic cells are identified by using fluorescent microscope. Detailed procedure of TUNEL assay is as follows:

1. Preparation of primary cells culture as described in earlier methods.

2. Induction of apoptosis in primary cells culture is done by treatment of primary cells culture with different concentration of xenobiotics and incubation in CO2 incubator for 12 h as mentioned in micronuclei assay method.

3. The positive and negative control cells provided with the kit are already f ixed. However, negative control cells are prepared from control sample with absence of inducing agent.

4. Cell Fixation: Cell fixation is a required step in the TUNEL assay and it is done according to the procedure mentioned in the supplied kit by using paraformaldehyde solution. Suspend 1–2 × 106 cells (treated) in 0.5 ml of phosphate-buffered saline (PBS) and the cell suspension into 5 ml of 1% (w/v) paraformaldehyde in PBS and place on ice for 15 minutes.

5. Centrifuge the cells for 5 minutes at 300 × g and discard the supernatant.

Technique to collect fresh and hygienic inflorescence sap (Neera) from coconut

In the traditional method of collecting sap, the inflorescence to be tapped is connected with an earthen pot/bamboo receptacle for the sap collection. Spadix to be tapped is tied with plastic rope and a small portion is sliced off from the top. The spadix is gently stroked with a mallet in the morning and evening. After a period of 8 to 10 days, sufficient sap starts oozing out from the cut end. A type of clay soil, a gummy material or leaf extract is pasted on the surface and sides of the cut end. Since the sap is highly nutritious, both enzymatic and microbial fermentation takes place. The total sugar reduces progressively and it becomes more acidic and develops a harsh odour and becomes unpalatable. In order to prevent fermentation, lime is coated inside the pot. The above method is not hygienic and attracts lot of insects, ants and contaminates from pollen and dust particles. In order to collect the sap fresh and hygienic, a simple device was developed.

7.1. Origin and distribution

• Native of South East Asia, Central America.

• Mexico - largest producer of diosgenin.

• In India, it is grown in North eastern states and Goa

• Dioscorea alata and Dioscorea esculenta - edible tubers.

• Dioscorea floribunda, Dioscorea villosa, Dioscorea composita - widely grown

• Major producing countries - Mexico, Guatamala, Costa Rica, India and China.

Injudicious and indiscriminate use of several agrochemicals viz. pesticides, herbicides, insecticides, dyes and medicaments in the field of agriculture and veterinary practices threatens the human and animal population for the toxicity of these environmental deterrents. These agrochemicals have been associated with several episodes of accidental poisoning and mortality among human and animal population. The episode of Mass ethion poisoning in Gujarat is one of the commonest example of inclusion of pesticides or agrochemicals in the environmental pollutants. There are modern tools to evaluate and analyze these chemicals in the environmental pollutants of human and animal use. The sensitivity of these modern highly sophisticated tools has now immensely changed the analytical environmental pollutants industry. Gas chromatography and Mass Spectrophotometry i.e. (GC-MS) technique is employed to detect the volatile residues from environmental pollutants. Whereas, High performance liquid chromatography i.e. HPLC is employed to analyse and detect non volatile drug residues. Gas Chromatography/Mass Spectrometry (GC/MS) instrument separates chemical mixtures (the GC component) and identifies the components at a molecular level (the MS component). It is one of the most accurate tools for analyzing environmental samples.