eChapters

Leaf Curl

Taphrina deformans

Disease Symptoms

• Initially yellowish to reddish areas appear on leaf blade of young leaves in the spring.

12.1. Tea

12.1.1. Algal leaf spot: Cephaleuros virescens

Symptoms

• Leaves develop lesions that are roughly circular, raised and purple to reddish brown.

Management

• Removal of infected portions and spraying of Bordeaux mixture

• Improving soil nutrient status by application of nitrogen, phosphorus and potassium

12.1.2. Brown blight, grey blight: Colletotrichum sp., Pestalotiopsis sp.

Introduction

Pomegranates are a tasty, juicy, sweet fruit that are rich in nutrients. Punica granatum is its scientific name, and Iran is where it was originally found. Because pomegranates may be cultivated effectively in regions of the nation with dry climates, they hold a distinctive place among the fruits that are grown there. Other names for pomegranates include Dadim in Sanskrit, Bedana in Bengali, Anar in Hindi, and Madulai in Tamil. The states of Maharashtra, Gujarat, Rajasthan, Karnataka, Andhra Pradesh, Tamil Nadu, and Uttar Pradesh are the primary locations for its cultivation. From the perspective of export, this fruit is also highly significant.

Pomegranate is referred to as a “Super Food” because of its many health and nutritional advantages. It is one of the greatest sources of antioxidants and has significant medicinal and nutritional value. Ripe fruit has a pleasant and nutritious juice. Juice from pomegranates in the summer, sherbet is incredibly refreshing. Pomegranates are regarded as a medication for long life and excellent health in the Ayurvedic medical system.

Late Blight

Phytophthora infestans

• Phytophthora in Greek means “plant destroyer”(phyto=plant, phthora= destroyer).

Introduction

Potato (Solanum tuberosum) is a widely cultivated and essential staple crop globally, known for its versatile culinary applications and nutritional value. With the exception of Kerala, potatoes are grown in practically every state in India under a variety of climatic circumstances. During the brief winter months, which span from October to March, 82% of potatoes are grown in plains. In West Bengal, potatoes are the most widely grown crop after grains (Chakraborty, 2012). Originating from the Andes region of South America, potatoes have become a crucial part of diets worldwide, providing carbohydrates, vitamins, and minerals. With a rich history dating back to ancient civilizations, including the Irish potato famine, this crop has played a significant role in shaping agricultural practices and food security.

GREEN GRAM (Vigna radiata) & BLACK GRAM (Vigna mungo)
Fungal diseases
1) Seed and seedling rot
Symptoms: The seeds rot before germination and after germination the seedlings also show rotting. The disease can be easily identified by poor germination and poor stand of the crop.
Causal organisms
i) Fusarium sp
ii) Pythium sp
iii) Rhizoctonia solani
iv) Macrophomina phaseoli
 

Powdery mildew

Sphaerotheca pannosa var. rosae (Sphaerotheca pannosa)

Diagnostic Symptoms

• A white, powdery fungal growth on the upper, lower or both leaves and shoots.

Introduction

Roses, widely cultivated as cut flowers in greenhouses, as entire plants in nurseries, or within home gardens, encounter common issues across these diverse growing environments, including black spot, Botrytis blight, downy mildew, powdery mildew, and rust. Specific challenges like downy mildew and rose rosette gained prominence in the 2010s. The susceptibility of roses to various diseases varies by geographical region; for instance, rose replant disease is a concern in Europe but not in North America. Greenhouses and tropical climates, as seen in Central and South America, face heightened issues with powdery mildew, downy mildew, rust, and Botrytis blight. Disease management proves costly and can constrain rose production, prompting innovations like the adoption of hydroponics in Colombia, leading to improved pest control, enhanced rose quality, and increased productivity.

1. Ginger (Zingiber officinale)

‘Ginger’, which originated in Southeast Asia has a long history that goes back over 5000 years when the Indians and Ancient Chinese considered it as a tonic root for all ailments. At an early date it was exported to Ancient Rome from India and was used extensively by the Romans. Subsequently it was cultivated in several other countries for its medicinal value. Ginger is cultivated in most of the states of India. Kerala and Meghalaya are the major ginger growing states. India’s production of ginger constitutes about 50 per cent of the total world’s production.

Ginger is an erect, herbaceous , perennial, flowering plant, the rhizome of which is used as a spice. Ginger is an ancient spice and is a common ingredient in many Asian foods and is used in soups, curries, noodles, stews etc. It is widely used to flavor beverages, such as tea, coffee, lemonades, cocktails etc. In many places across the world ginger is used in sweets, alcoholic beverages, such as ginger beer and wine. Ginger has powerful anti-inflammatory properties. Further it has many other health related benefits, such as ability to help with arthritis, osteoarthritis, relieves nausea and pain, prevents cancer, improves respiratory conditions and reduces flatulence. It also boosts bone health, strengthens the immune system and increases appetite.

8.1. Pepper

8.1.1. Phytophthora foot rot / quick wilt: Phytophthora capsici

Symptoms

• Die back

• Discoloration occurs and rotting progress upwards and downwards resulting in die-back.

• Lateral branches of affected vines break off at nodes and fall off.

• Foot rot or collar rot

• Stem near ground level get infected and rotting and death of vine within 2-3 weeks.

• Affected portion emits bad odour.

• Root rot

• Infection starts at main root or at feeder root.

• Leaves become yellow and defoliate.

Leaf Spot

(Mycosphaerella fragariae teleomorph, Ramularia brunnea Peck anamorph)?

Disease Symptoms

• Older leaves usually show purple spots (3-6 mm in diameter) on the upper surface, sometimes with a slightly darker halo

SUGARCANE (Saccharum officinarum)
Fungal diseases
1) Red rot
Symptoms: It is one of the most destructive disease of sugarcane. Dark reddish spot with brown margin appear along the mid rib. When the diseased canes are split open red blotches with transversely elongated white centres are seen. Under humid conditions numerous black dot like acervuli develop in the central discoloured portions. The diseased tissues emit a typical alcoholic odour. The juice of the affected stalks gives a sour alcoholic smell.
Causal organism: Colletotrichum falcatum
Management
i) Select healthy and disease free setts.
ii) Dip the setts on Carbendazim 50% WP or Thiophanate Methyl 70% WP @0.15% solution for 30 minutes before planting.
iii) Avoid rationing of infested crops.
iv) Growing resistant/tolerant varieties, i.e. Co6907, Co 7219, Co838,
 

Diagnostic Symptoms

• Practically all above ground parts are affected, but stalks are the main targets of stack.

• Spindle leaves (third or fourth) starts drying.

• Further stalks becomes discolored and hollow.

Diagnostic Symptom

• Circular to oval, dark brown to black spots appear on the leaves.The spots enlarge into round to irregular spots with concentric rings and coalesce causing blighting and withering of leaves.

• These are surrounded by chlorotic zone with grey white necrotic center.

Introduction

Tomato scientifically known as Solanum lycopersicum L. is a widely cultivated and consumed fruit that is often mistaken for a vegetable due to its culinary uses. Belonging to the Solanaceae family, tomatoes are native to western South America with their domestication dating back to ancient civilizations in Mesoamerica. Tomatoes are prized for their vibrant red colour, juicy flesh, and rich, slightly sweet f lavour. They are a versatile ingredient used in a wide range of culinary dishes, from salads and sauces to soups and sandwiches. Tomatoes are a significant source of essential nutrients, including vitamin C, vitamin K, potassium, and folate. Additionally, they contain the antioxidant lycopene, which is believed to have various health benefits.

Leaf spot

Colletotrichum capsici

Diagnostic Symptoms

• Initially oblong brown spots having about 4-5 cm in length and 2-3 cm in width with grey centres surrounded with yellow hallo are found on the leaves .

Important diseases of vegetable crops

1. Damping off: (Phytophthora parasitica, Pythium sp. Rhizoctomia solani):This is a disease of the seedlings at the nursery stage. Due to the attack of this fungus seedlings rot at the ground level and collaps.

Rust

The rust of wheat has been studied more than any other disease in the world. In India, K.C Mehta and his associates studied this disease with exemplary zeal. Three rusts of wheat are known. These are Black stem rust, caused by Puccinia graminis tritici, Brown rust (leaf rust), caused by Puccinia recondita, and Yellow rust, caused by P? striiformis. Brown and yellow rusts cause more damage thant the black rust, because of early appearance in the season.

Distillation of rose oil and rose water:

For distillation of rose oil and rose water initially the fresh rose flower are hydro distilled. The oil and water are collected together. The oil which floats upon the water is separated by decantation get oil directly, while the water is re-distillated. The oil obtained from re-distillation is called recovered oil or water oil. The direct oil and water oil are then combined to make the commercial rose oil.

The water obtained from the second re-distillation step is the commercial rose water

ABSTRACT

The genus Aconitum is in the limelight of the current scenario of the pharmacopeia and has became the centre of attention in the field of herbal medicines, as is manifested with various biological and pharmacological cores which make the genus most imperative. For vast decades some species of the genus Aconitum and its derivatives were considered as vizulently fatal but were liquidated as intact drug elites through traditional as well as modern system of medicine. The retrospection of the validated information in account of the genus Aconitum represent a pioneering approach to corroborate the scientific studies on almost everyaspect of the herbal genusAconitum, as a number of species belonging to this genus has been enlisted in the Red Data Book of IUCN which have been renowned for their medicinal benefits in various systems of medicine. The tubers some species of Aconitum contain lethal diterpene alkaloids such as aconitine, mesaconitine, and hypaconitine, which easily can be directed into screened harmless alkaloids such as benzoylaconine, aconine, and pyroaconine by heating or alkaline treatment. Currently, the processed tubers are widely and safely practiced for the remedyof pain, neuronal disorders, inflammation, and rheumatism and have a huge therapeutic index for curing ailments like hysteria, throat infection, dyspepsia, abdominal pain, diabetes and also as a cardiac depressant in massive arterial tension of cardiac origin. An important component extracted from some species of Aconitum, Lycaconitine has been found to be effective against multi-drug resistant cancers. The di-terpenoid alkaloids of aconite source and their increasing demand for the active compounds used in different systems of medicine has led to overharvesting of the tubers, resulting in rapid depletion of the natural stocks of this valuable plant. As a consequence, the genus is now on the list of rare and threatened species. Since the commercial demand of this important medicinal plant is very high and hence needs to be conserved as the species density of this genus has been threatned due to many factors. As such diverse efficient and standardized protocols for its regeneration like micropropagation, somatic embryogenesis and some other conventional efforts like storage of seeds as a means of ex-situ conservation must be attributed on the aspects of conservationof genusAconitum. The exploitation of population genetic diversity will provide useful information for the biological conservation of these esteemed species of Aconitum in the regions . Since a complete revision of the genus Aconitum in the region has not yet been carried out and the high level of endemism and a lack of relevant information of the genus Aconitum have made its various scientific studies a very interesting object. The scientific and systematic information about the genus Aconitum generated demands validation as only a little attention has been done on its various aspects from cultivation, conservation, phytochemistry and pharmacological evaluation to the molecular characterization. Consequently species specific measures are needed and a lot of important attributes has to be assigned to the species of this genus which will ultimately help to ensure simultaneously both conservation as well as sustainability in raw material production of the genus.

The arid zone of India covers about 12% of the country’s geographical area and occupies over 31.7 mha of hot desert and about 7 m ha under cold desert. The arid regions of Rajasthan, Gujarat, Punjab and Haryana together constitute the Great Indian Desert known as Thar Desert that accounts for 89.6% of hot arid regions of India. In Gujarat, 6.22 m ha area is under arid zone which constitute 19.6% of the arid area of country (Fig 1). In Gujarat, eight districts fall under arid zone, namely, Kachchh (100% of district area), Jamnagar (80%), Surendranagar (29%), Junagadh (20%), Banaskantha (18%), Mehsana (7%), Ahmedabad (6%) and Rajkot 6%).

1. Introduction

India, as a party to the Convention on Biological Diversity (CBD), has obliged to document a whole range of organism diversity within her territorial boundaries, make all attempts to conserve these bio-resources and monitor the efficacy of the conservation measures adopted. With its diverse ecological conditions and unique geographical location, Indian landmass supports rich diversity of algal flora. However, this algal wealth needs to be systematically surveyed and documented from all possible habitats. Ecological patterns emerged from recent studies within India are assessed with the identification of knowledge gaps and conservation implications in algal systematics.

During last one decade, great advances have been made in the field of Algology/Phycology throughout the world. Over the years algae has attained a greater reputation and popularity because of its economic importance, particularly as food, for commercial products, water contamination, toxin production, sewage oxidation, municipal water purification in fisheries and above all for the study of fundamental scientific information. But before the algae put in to such important use, it is imperative to have a baseline data about the material in question. Without knowing the status of algal resources of any area one cannot have projections for their utilization or gain knowledge of their ecological role and functions. The taxonomy of algae is, therefore, a prerequisite for all applied fields of algae apart from academic interest. Recent global surveys have revealed that there is lack of knowledge about fresh water algal diversity of tropical countries including India.

1. Introduction

The gymnosperms constitute a distinct subdivision of spermatophytes, the seed bearing plants. The term ‘gymnosperm’ is derived from Greek words, gymnos, naked and sperma, seed, which indicates that these are naked-seeded plants, i.e., those in which the seeds are not enclosed within the fruits but are borne freely exposed on an open carpel. In contrast in the angiosperms the seeds are enclosed within a closed carpel (ovary). The first known use of the term gymnosperm was given by Theophrastus in 300 BC in his book “Enquiry into Plants” to describe “plants with unprotected seeds”. Goebel (1905) rightly described gymnosperms as “Phanerogams without ovary”. The Scottish botanist, Robert Brown in 1825 first distinguished gymnosperms from angiosperms. At one time they were considered to be single class of seed plants, called Gymnospermae, but taxonomists are now tend to recognise four distinct divisions of extant gymnospermous plants - coniferophyta (Pinophyta), Cycadophyta, Ginkgophyta and Gnetophyta. Geological history reveals that all divisions of gymnosperms have been distinct groups since their origin.

Among the gymnosperms are plants with stems that may barely project above the ground and others develop into the largest trees on the earth. The extant gymnosperms are a small group of plants comprising ca. 1026 species belonging to 83 genera and 12 families (Christenhusz et al. 2011). They are distributed throughout the world, with extensive latitudinal and longitudinal ranges. Economically, gymnosperms are of outstanding value, fulfilling the greater proportion of our timber as well as resin requirements. They are also the dominant forest makers of the world and in addition they play a leading role in the preservation of the environment (Sahni 1990). By far the largest group of living gymnosperms is the conifers (pines, cypresses and relatives), followed by cycads and Gnetales (Gnetum, Ephedra and Welwitschia).

Turmeric (Curcuma longa L.) is a tropical herbaceous plant extensively cultivated in India, Sri Lanka, parts of China and Pakistan.  Country of origin is not known with certainity, it is either of Chineese or Indian origin. India ranks first in area (1.49 lakh ha.) and production (5.27 lakh tonnes.) in the world and it is grown in most of the states in the country.  Major turmeric growing states are Andhra Pradesh (56,000 ha.), Orissa (23,000 ha.), Tamilnadu (17,000 ha.), Assam (12,000 ha.), West Bengal (11,000 ha.) Maharashtra (6,000 ha.)  Karnataka (6,000 ha.) and Kerala (3,000 ha.).  Andhra Pradesh ranks first in area and production in the country. Area under this crop is increasing in North Eastern States.

Turmeric belongs to family Zingiberaceae and genus Curcuma. Genus Curcuma has 42 species, of which Curcuma longa is extensively cultivated for culinary purposes, Curcuma aromatica for flavour and recently Curcuma amada (mango ginger) for preparation of chutneys. Turmeric is mainly cultivated for under ground rhizomes, rich in curcumin. Turmeric is traditionally used for medicinal, religious, culinary purposes and also as a cosmetic and dye (Shah 1997). Recent reports in controlling human cancer has given renewed interest on the crop. In Ayurveda, turmeric is regarded as aromatic, a stimulant tonic, carminative and anti helminthic. Essential oil of turmeric is antiseptic and is used in treating gall stones and gall complaints (Pruthi 1976).

ABSTRACT

The current paper discusses the diversity, distribution and commercial importance of Indian Magnolias. Globally, these species are found in tropical, subtropical and temperate forests of southeastern Asia and tropical America. In India, there are 25 species of Magnolias and most of them are distributed in Northeast India, with highest number in Assam. Of all the species, two (Magnolia gustavii and M. pleiocarpa) are critically endangered, one (M. pealiana) endangered, two (M. manii and M. nilagirica) vulnerable, nine least concern and ten data deficient at global level. The highly threatened nature of most of the magnolias species calls for their immediate conservation and protection measures. Members of this genus are known to be rich in a wide variety of biologically active compounds including alkaloids, flavonoids, lignans, neolignans and terpenoids. Many of the species have been found to possess potent procognitive activity, anti-oxidative, anti-microbial, anti-inflammatory, anti-angiogenic, diuretic, anti-ulcer, analgesic, anti-helmintholytic, and anti-cancer activities. The species have huge economic potential and are used for a number of purposes including ornamental, medicinal, culinary, timber and joinery works.

Abstract

Lianas are slightly lesser known angiosperms, grows typically in the form of climbing vines mostly in tropical and subtropical forests. Such plants are characterized by scandent thick stems, woody stem in nature and vary in shape and size. They depend on supports in the form of trees, fence and usually climb upward towards the sunlight as they needed for their survival. Keeping the importance of lianas in forests and household, this study was conducted to document the liana angiosperms growing in Western Himalaya along with their medicinal potential for human and animals. This study reveals a total 64 lianas species belonging to 24 families recorded from Jammu and Kashmir, Ladakh, Uttarakhand, Himachal Pradesh and Punjab. This investigation also provided with medicinal potential of these species, which is used as ethnomedicine by local people living in the interior regions of hills and mountains of Himalaya. In this communication, we highlight lianas as an example of an underestimated forest element that, on the one hand, is a key component in and around forests. This study also helps in conservation of endangered liana species in Himalaya.

1. Introduction

Fungi are ubiquitously present in air, water, soil and organisms, or on organism surfaces. They are a group of eukaryotic system that are of great applied and scientific interest to common man, microbiologists, agriculturists and industrialists. They are a diverse group of organisms with respect to taxonomy, size, morphological appearance, mode of nutrition and reproduction. Fungi are eukaryotic spore bearing protists that lack chlorophyll. Fungi comprise the molds and yeasts. Yeasts are usually unicellular measuring only several microns in diameter, whereas molds are filamentous and multicellular which may grow up to several meters in diameter like the extremely large polypores. Fungi generally reproduce both sexually and asexually (Gow and Gadd 1995).

Although it is not easy to define fungi, biologists currently use the term “Fungus” to include nucleated, spore bearing, achlorophyllous organisms with absorptive nutrition which generally reproduce sexually and asexually, and whose filamentous branched somatic structures are typically surrounded by cell walls containing cellulose of chitin or both. A feature which all fungi have in common is their mode of nutrition. Like animals, fungi are heterotrophic organisms, which must consume preformed organic matter. They may live as saprophyte or parasites. They are of great economic importance to man and play an important role in the disintegration of organic matter and affect us directly by destroying food, fabric, leather and other commercial goods. They are responsible for a large number of diseases of plants, animals and man.

Horticulture is a wide field and includes a great variety and diversity of crops. In general, these crops are classified as follows:

1. Crops grown for the food and/or vitamin and mineral content, for e.g., apple and sweet potato.

2. Crops grown for their beverage properties, for e.g., tea and coffee.

3. Crops grown to beautify the environment, for e.g., the grasses of the well planned lawn.

4. Crops grown to express sentiments, for e.g., cut roses, jasmine.

5. Crops grown for perfume for e.g., rose, jasmine.

6. Crops grown for spices for e.g., pepper, nutmeg.

Delineating species boundaries correctly and also identifying species are crucial to the discovery of life’s diversity because it determines whether different individual organisms are members of the same entity or not (Dayrat, 2005). The identification of species depends on the knowledge held by taxonomists whose work cannot cover all taxon identification requested by non-specialists. To deal with these difficulties, the ‘DNA Barcode of Life’ project aims to develop a standardized, rapid and inexpensive species identification method accessible to non-taxonomists.

DNA barcoding is a technique in which species identification is performed by determining and comparing the sequences of several hundred base pairs of nucleotides from a small fragment of an identified gene, with the aim of contributing to a wide range of ecological and conservation studies in which traditional taxonomic identification is not practical (Krishnamurthy and Francis, 2012). The two main ambitions of DNA barcoding are to assign unknown specimens to species and to enhance the discovery of new species and facilitate identification, particularly in cryptic, microscopic and other organisms with complex or inaccessible morphology (Hebert et al., 2003a). Apart from these, identification of species previously defined by other criteria to also is an objective of barcoding. Another major objective could be the definition of operational units for ecological studies. This may also be used for forensic purposes by linking the specimens with already identified databases.

ABSTRACT

DNA fingerprinting is increasingly being used for identification of varieties, quantification of similarity between varieties and study of genetic diversity available in the population.

This is all the more important in crops where morphological variability is less or one variety is known by different names or different varieties are known by same name.

DNA fingerprinting using RAPD method has been standardized for tapioca. Dellaporta method has been used for extraction of DNA and amplification was done following William’s method. This technique has been used for molecular characterisation of accessions and identification of duplicates in the germplasm maintained at Central Tuber Crops Research Institute, Thiruvananthapuram. This technology has been extended for DNA fingerprinting of released varieties and also some of the elite breeding lines.

Eleven varieties released from the institute were fingerprinted using a few primers, which showed high polymorphism in tapioca. One single primer gave distinct banding pattern for all the 11 varieties and it was capable of earmarking each variety.

Individual variety was finger printed for 20 different primers and the results were documented. This technique is being extended to promising land races as well. DNA fingerprinting will be useful for protecting Plant Breeders’ Right as well as Farmers’ Right in the new Patent Regime.

Apoptosis is triggered by extrinsic and intrinsic stimuli as radiation, oxidative stress and genotoxic chemicals. It is defined by characteristic changes in the nucleus morphology including chromatin condensation and fragmentation, overall cell shrinkage/rounding and formation of apoptotic cell bodies. Among the many markers of apoptosis, DNA fragmentation is considered a hallmark. The DNA ladder assay described here is a simple, sensitive, cost-effective and rapid method for estimating apoptosis in cells.

Isolation of Genomic DNA

Genomic DNA from primary cells culture of PMNC and splenocytes of rats is isolated by using standard phenol-chloroform DNA isolation protocol of Sambrook and Russel (2001) as described below:

1. Preparation of primary cells culture of different cells, namely-PBMNCs, thymocytes and splenocytes form Wistar rats and viability checked by trypan blue dye exclusion test.

2. 106 cells/ml of primary cell culture is used and seeded in 24 well culture plates containing along with treatment with xenobiotics at different µM concentration.

3. Cell culture plates are incubated in CO2 incubator at 37°C with 5% CO2 for 12 hrs.

4. The cells are collected in 1.5 ml of micro centrifuge tube and centrifuged at 3200 rpm for 10 min at 4°C; supernatant is discarded and pellet is washed with PBS (pH 7.2).

5. DNA extraction buffer (500 µl/tube) is added in tubes. Gently tap to disperse the cell pellet in extraction buffer and keep in water bath for 1.0 hr at 37 °C (it is to ensure that pellet is completely suspended so that cells are accessible to SDS and Proteinase K).

6. 10 % SDS is added (20 µl/ml) to cell suspension and the tubes are gently mixed by inverting the tube. The contents of tube become viscous which indicates lysis of cells. After that sample is handled gently from onward to avoid shearing of DNA.

7. Proteinase K (15 µl) of 20 mg Proteinase K/ml of buffer is added in tubes in two pulse i.e. half the requirement is added to tube in 1st pulse, mixed gently end to end and kept in water bath at 50°C. After 3-4 hrs, the second pulse of the remaining amount of Proteinase K is added and tubes are incubated at 50°C overnight.

8. Equal volume of equilibrated phenol (Tris saturated phenol pH> 7.8 ) is added in the tubes next day morning. Mix the contents of tube gently by inverting the tube for 15 min till a light coffee coloured uniform solution without any balls of phenol is formed and then centrifuge at 3400 rpm for 15 min.

ABSTRACT

The process of developing new crop varieties involves many steps and takes several years. In recent times, biotechnological advances have helped in reducing these considerably. Apart from the techniques of haploid culture and genetic engineering, one of the tools which is making it easier for scientists to select plant traits and develop new varieties is the use of molecular markers. Molecular markers can be used for (a) characterization of germplasm (b) varietal identification and clonal fidelity testing (c) assessment of genetic diversity (d) validation of genetic relationships and (e) marker- assisted selection. These have widespread applications in management of genetic resources as well as in breeding programmes. Marker-assisted selection has practically revolutionized breeding programmes. Traditionally, plant breeders have selected plants based on their visible or measurable traits called the phenotype. But this process can be difficult especially in the case of multigenic or quantitative traits. Molecular markers can be used to trace linkages with traits of importance. In the area of horticulture, this is a very useful tool in tree breeding, where the breeder has to wait for several years for phenotypic expression of the desired trait. A number of DNA marker technologies are now available and rapid advances are being made in the development of more sensitive marker technologies. The techniques involved, the various applications of DNA marker technologies in horticultural crops and future perspectives in the national context have been discussed.

A laboratory is essential for research work in science and development. Most of the research institutes have dedicated laboratories for researchers and students to conduct experiments, perform research work, as well as to provide hands-on learning/training to students. There are certain rules and regulations laid down by the laboratory in-charge which need to be followed strictly for safety purposes. Some of the laboratories keep ‘safety manual’ for researchers to read before entering into the laboratory to conduct experiments. Whenever safety is compromised in a laboratory, there is increase in risk of unwanted incidents in the laboratory. Storing food/beverages in the same refrigerator where laboratory chemicals are kept may lead to disastrous effects on health. This chapter aims to provide instructions in brief on the things to be performed (Do’s) before and after entering onto a laboratory as well as the things never to be done (Don’ts) in a laboratory for the safety reasons.

Good Laboratory Practices (Do’s)

• P Wear apron/labcoat while working in the laboratory.
• P Use gloves/respiratory mask/fumehood while handling toxic/volatile/inflammable chemicals/reagents.
• P Use pippet or pippetor for safety in transfer of solutions.
• P Protect eyes, face and naked parts of the body from UV radiations. Use safety goggles or face mask/apron.
• P Keep your eyes away from Liquid Nitrogen and use cryogloves while handling it.
• P Arrange all the necessary materials/solutions before starting the experiment.
• P Periodically fumigate/ozonize culture and inoculation rooms.
• P Autoclave all contaminated materials before discarding.
• P Pre-treat gel or solution containing Ethidium Bromide (EtBr) before discarding into laboratory waste-disposal bin, and use gloves while handling them.
• P Burn all the transgenic plant materials in an incinerator after taking necessary observations.
• P Make entry in the designated log-book and clean the instrument after using it properly.
• P Switch-off the instrument after use or when not in use.
• P Wash hands with soap and remove the labcoat before leaving/going out of the laboratory7.
• P Ensure that switches for fans/lights, and water taps are off while leaving the laboratory.
• P Follow the biosafety guidelines suggested for handling and disposal of the hazardous chemical.

1. Introduction

Phytochemists and pharmacologists from all over the world obtain several patents for herbal drugs developed on the basis of the indigenous knowledge systems of different ethnic groups. Many countries rich in traditional medicinal knowledge have been conducting ethnobotanical survey of medicinal plants (Patil and Bhaskar, 2006). In India the native people have exploited a variety of herbal medicines for effective curing of various diseases. The plants used, preparation and administration of drug varies from area to area (Rajaram, 2006). The ethnobotanical research still plays its evident scientific role in stimulating further phytochemical and pharmacological studies (Leporatti and Corradi, 2001).

The objective of this study is to interact with Kani traditional healers and document their traditional knowledge. The Kani-tribals are one of the most primitive hill tribes of Western Ghats. They occupy the slopes of the Agasthiarmalai in Tirunelveli, Tamil Nadu, India. Some ethnobotanical studies have been done in this area (Anonymous, 1948-76, Chopara et al., 1956, Satyavati, 1976, Jain, 1981, Nagendra Prasad et al., 1996). The impact of deforestation, urbanization and modernization makes the tribals shifting from their natural habitats to urban areas. Documenting the traditional knowledge through ethnobotanical studies is important for the conservation of medicinal plants and traditional medicines.

In literal sense a species is called underutilized whose potential has not been properly realized. These underutilized crops (referred to also by other terms such as minor, orphan, neglected, underutilized, underexploited, underdeveloped, lost, new, novel, promising, alternative, local, traditional, niche crops) have been included in world-wide plans of action after having successfully raised the interest of decision makers. Wealth of plant genetic diversity currently available holds vast potential. We use only small number of crop plants for our basic requirements. Many thousands of wild plants have great economic and cultural importance and tremendous market potential for vast numbers of people throughout the world. But the majority are still unknown to science. Plant genetic resources have gained global attention in  recent past by agricultural and environmental institutions. Conservation and use of genetic resources attracted  considerable attention. Value of biological diversity in economic terms is very important for human society.

Abstract

Pomegranate, Punica granatum L., is an ancient fruit tree native to the Middle East. All plant parts are rich source of the various secondary metabolites that accounts for its antioxidant and health-promoting activity. A strong genetic control on anthocyanin production is responsible for variability in the color of peel and arils of pomegranate. Characterization, cloning and expression of the genes related with anthocyanin and tannin production provides routes for engineering of these complex pathways. Recent studies on pomegranate genome sequencing have elucidated several biochemical pathways and candidate genes that could assist to increase production of potent bioactive compounds in near future. This will further facilitate our understanding to some exclusive biological processes that plays a significant role in pomegranate breeding.

Agriculture University Kota, Jhalrapatan, Rajasthan, India

1. Introduction

India’s population is likely to reach 1.7 billion by 2050, making it as the thickly populated nation in the world. To feed the ever rising population, food production need augment by 70%. This augment is very serious since Indian agriculture is facing numerous challenges due to disintegration of holdings, ill brunt of climate change, sustaining investment, soaring fluctuation in prices and production, unorganized markets, inconsistent market performance, renovation of agricultural land intended for non-farm purposes and enlarged cost of production (Gangaiah, 2018). The current conditions of the Indian agriculture sector reflect that the farmers are facing severe deficit of income which is to an imperative issue and as a result dissuading the rural youth to take up agriculture as livelihood operation.

Dragon fruit  (Hylocereus undatus (Haw.) Britton and Rose) is a climbing vine cactus species which has received worldwide recognition, first, as an ornamental plant and then as a fruit crop. Its fruit is the most beautiful in the family Cactaceae with a bright red skin studded with green scales and white or red flesh with tiny black seeds.  The flower is so beautiful that it is nicknamed as Noble Woman or Queen of the Night.  The juicy flesh of the fruit is delicious in taste. It is well established as a new crop in Australia, China, Israel, Malaysia, Nicaragua, Taiwan and Vietnam. In Vietnam, it has become a major export crop, which fetches higher prices than even Durian, the King of Fruits in south East Asia.  The main constraint is that the establishment cost is high due to use of trellises for climbing. The cost of establishment will depend on the type of trellises used, and experience in Sri Lanka shows that relatively cheap trellising is sufficient. The other agronomic practices are easy and less expensive; maintenance cost is low and aftercare is minimal due to fewer pest and disease attacks. Further, the biggest advantage of this crop is that once planted, it grows for about 20 years, and one hectare could accommodate about 800 Dragon fruit plants.

Micro irrigation normally called as drip irrigation was introduced in early seventies. Drip irrigation is one of the advanced methods of irrigation, by which water can be supplied directly into the root zone of the soil to achieve considerable saving of water through high water use efficiency, compared to surface irrigation method where water use efficiency is low due to losses in the water distribution system in the field. In micro irrigation method, water supplied at slow rate that is 1.0-2.0 kg/sq cm to the root zone of an individual plant through lets called emitters or drippers fitted with laterals pipes and filters at pump side. The success of micro irrigation is proven and efforts are being made in promoting the same extensively among the farmers in arid areas.

The hot arid regions of India are spread over about 31.7 million ha area mainly in the States of Rajasthan, Gujarat, Andhra Pradesh, Punjab and Haryana, which inhabit on an average 61 persons per square km making up a population of nearly 20 million peoples. The Indian arid zone is characterized by high temperature and low and variable precipitation which limit the scope for high horticultural productivity. However, these conditions greatly favour development of high quality production in number of fruits such as date palm, ber, pomegranate, citrus, aonla, bael, grapes and, guava. The optimized technologies and inputs could increase the existing low productivity. It is now realized that there is a limited scope for quantum jump in fruit and vegetable production in the traditional production areas. The amelioration of the extreme conditions is also considered vital for life support to the inhabitants of this area. The recent awareness regarding the potential of these ecologically fragile lands for production of quality horticultural produce has not only opened up scope for providing economic sustenance for the people of this region, but also for bringing new areas to increase production through horticulture.

Medicinal plants and their various products are extensively utilized not only in traditional systems of medicine but also in modern medicine throughout the world. In recent times, they have also attracted attention of scientists encompassing various disciplines of medicine and biotechnology for search of new drug molecules and modern drug development. Although several research strategies are followed for drug development only the traditional, empirical and molecular methods/strategies are mostly applied. There is also need of scientific validation for safety, efficacy and rational use of traditional form of medicine along with development of standards for identity, purity and strength (Singh and Bagchi, 2002).

Approaches of Drug Developement

Following approaches are generally employed to discover and invent new therapeutic agents from plants viz. screening of plant materials, identification of active constituents, pharmacological and clinical evaluation and drug design.

Tissue Collection

Rats are most commonly employed as test subjects to conduct the experiments related to drug metabolizing enzyme assays involving various enzymes i.e. microsomal and non microsomal enzymes. Rats are humanely sacrificed at the end of the exposure period of 28 days of any therapeutic agents or drug and the liver of all the animals has to excise and immediately wash with ice-cold normal saline, weigh and perfuse with ice-cold 1.15% KCl solution containing 0.05 mM EDTA. Livers are processed further for preparation of microsomes for determination of microsomal drug metabolizing enzymes.

Preparation of Microsomes

Livers tissues are homogenized in 3 volumes (1:3) of ice-cold (1-4o C) homogenization buffer containing 1.15% potassium chloride in 50 mM potassium phosphate buffer solution (pH 7.4). The homogenates are centrifuged at 15000 g for 30 min (Hitachi cs120GX II). The mitochondrial supernatant (8 ml) is transferred to ultracentrifuge tubes and spun at 105000 g for 1 hour in ultracentrifuge to sediment the microsomal pellet. Cytosolic fractions are collected from the middle separating the lipid layer on top in separate micro centrifuge tubes. The microsomal pellet is then washed by adding 8 ml of resuspension buffer (homogenization buffer containing 0.05 mM EDTA) and spun again 105000 g for 15 min. The microsomal pellet so obtained is resuspended in 4 ml of resuspension buffer and 1 ml aliquots are stored immediately at -80o C until assayed for drug metabolizing enzymes.

Moringa (Moringa oleifera) is one of the most popular perennial vegetables grown in South India. It is commonly grown as a unique vegetable. Its fruits, leaves and flowers are equally useful. They are cut into slices and used in the preparation of a number of food items. Moringa oleifera is the most widely cultivated species of a monogeneric family, the Moringaceae. The tree is indigenous to North West India. It is found wild in the sub Himalayan tract from the river Chenab eastward to the Sarda and in Tarai tract of Uttaranchal in India. Moringa is native to the sub-Himalayan tracts of India, Pakistan, Bangladesh and Afghanistan. This rapidly growing tree (also known as horseradish tree, drumstick tree, benzolive tree, kelor, marango, mlonge, moonga, mulangay, nébéday, saijhan, sajna or Ben oil tree, radish tree, West Indian ben), was utilized by ancient Romans, Greeks and Egyptians. It is now widely cultivated and has become naturalized in many locations in tropics. It is a perennial softwood tree with timber of low quality, which for centuries was advocated for traditional medicine and industrial uses. The name drumstick is derived from the pod shape resembling slender and curved stick used for beating the drum. Fruits also resemble the siliqua of radish and hence the name “radish tree”.

12.1. Introduction

Drying refers to the method of removal of moisture content from the food to a level at which the activities of food spoilage and food poisoning micro-organism are inhibited. Drying under open sun is probably the oldest method of food preservation used for agricultural crops including food grains, oilseeds as well as fruits and vegetables. Most of the fruits and vegetables contain enough moisture to permit the activity of enzymes and micro-organisms for spoilage and drying is necessary to reduce the water activity. Therefore, reduction in water activity of the food is the main principle of preservation by drying. Two commonly used terms for dried foods are low moisture foods and intermediate moisture foods (IMF). During drying, a single layer of fruit and vegetables, either whole or sliced after primary pretreatments is spread on trays which are placed inside the dehydrator or in the open sun for drying. In mechanical dehydrator, the initial temperature is generally kept at 43oC which is then gradually increased to 60-66oC for vegetables and 66-71oC for fruits.

Drying- Removal of water from product by influence of non conventional energy source like sun and wind.

Dehydration- Process of removal of moisture by the application of artificial heat under controlled condition of temperature, humidity and airflow etc.

Advantage of dehydration over sun drying

The process of dehydration is much more rapid than sun drying.

Dehydration requires less floor area.

Dehydration is done under very hygienic condition.

Sun drying is not possible in cloudy weather or during rain while dehydration or mechanical drying is not dependent on weather.

The colour of dehydrated or mechanically dried fruit and vegetable remain uniform due to uniform drying temperature.

Introduction

While experimenting with chemicals, sometimes we need to dry the chemical/ solution (solid, liquid or gas) using a process like evaporation or distillation, mainly to remove water or different solvents. Drying is the process of evaporation or distillation to remove water/solvent from a solution, solid or even from gas. Generally, a drying agent (usually an anhydrous inorganic salt) is used for this process, which reacts with the water (present in the chemical/ solvent to be dried) to form a hydrate. For example, anhydrous MgSO4 is one of the drying agents. The drying agents are recognized by their capacity (the rate at which they absorb water) and their intensity (the amount of water left behind in the solvent at equilibrium). No chemical reaction or change in phase occurs during the drying process.

Type of Solvents

Apart from water (a universal solvent), there are three families of organic solvents used in laboratories.

1) Oxygenated solvents: Alcohols, Ketones, Glycol ethers, Glycol esters, etc.

2) Hydrocarbon solvents: Aliphatic and Aromatic hydrocarbons.

3) Halogenated solvents: Chlorinated hydrocarbons.

Introduction

Flowers are associated with mankind since the dawn of civilization. Flowers are required for all the functions, ceremonies, rituals and celebrations from dawn to the dusk. In India, flowers are mainly grown for cut flowers and loose flowers purpose. By using best postharvest techniques, we can preserve the flowers ranging from few days to few weeks, but drying or dehydration is a technique by which flowers can be preserved for months to years. India has rich biodiversity comprising of wide variety of ornamental trees, shrubs, climbers, etc. which are not being used as fresh or loose flowers but their branched, twigs, flowers, fruits, cones, stems, roots, bark, etc. can be dehydrated to preserve their beauty and for utilization in dry flower industry.

1. Introduction

Drying is a thermo physical and physicochemical process, which involves removal of excess water from a solid, semi-solid or liquid food material by evaporating it into vapour phase on applying heat, to reduce growth of microorganism or to completely eliminate their growth. Hence, reducing the water activity increases shelf life of food products. Drying medium for carrying evaporated moisture can be air or steam. Benefits of drying include increase in shelf life, easy to transport, handle food material and reduce packaging cost as it reduces bulk weight. 

DUS testing is a way of determining whether a newly bred variety differs from existing varieties within the same species, or the characteristics used to establish distinctness are expressed uniformly and that these characteristics do not change over subsequent generations. Testing the distinctness, uniformity and stability (DUS) of crop varieties is a statutory requirement before varieties can be entered into the Registers of Cultivars (National Lists) and/or granted Plant Breeders’ Rights (PBR). In India, Protection of Plant Varieties and Farmers’ Right (PPV&FR) Act (2001) provides the registration of new variety of plant if it conforms to the criteria of distinctness, uniformity and stability.
Why do we need a DUS test? New (candidate) varieties should be distinct from all other varieties whose existence is a matter of common knowledge, and also sufficiently uniform and stable with respect to the characteristics used to demonstrate distinctness. ‘Common knowledge’ is broadly defined to include all known varieties, i.e. any variety entered into or subject to an application for PBR, varieties grown commercially, held in publicly accessible reference collection, or of which there is a published description (UPOV 2002).
The responsibility for DUS testing of potato varieties in India lies with the ICAR-Central Potato Research Institute (CPRI), Shimla, which is the national institute for potato research and development. Currently a set of 51 DUS characters in potato are defined by CPRI and being used for distinguishing new variety.

ABSTRACT

Dysoxylum binectariferum (Roxb.) Hook. is an evergreen tree, indigenous to India and widely distributed in different parts of India. Primarily, this plant is used in the timber market for the preparation of furniture and other wooden goods. Apart from utility in timber market, it has tremendous medicinal properties because of its unique secondary metabolites. Its hydroalcoholic extracts possess a wide range of pharmacological activities including immunomodulatory, anti-inflammatory, anti-cancer, anti-leishmanial, etc. The chromone alkaloid rohitukine, the major constituent of the plant, possess anti-fertility, anti-leishmanial, anti-adipogenic, anti-ulcerogenic, anti-cancer and immunomodulatory activities. The most extensively studied and advanced therapeutic property of this chromone alkaloid is its cyclin-dependent kinase inhibition activity, which has a direct link with its anti-cancer effect. Inspired by this natural product, two clinical candidates (flavopiridol and riviciclib) are discovered and studied in human cancer patients for treating various types of cancer. Another preclinical lead IIIM-290, which is directly derived from rohitukine via synthetic modification, has demonstrated promising oral efficacy in animal models of various cancer types. The high-value medicinal applications of this plant have necessitated the need of bringing this tree under captive cultivation. The present chapter discusses the biology pharmacological and secondary metabolites present in Dysoxylum binectariferum. The preclinical and clinical leads derived or inspired from rohitukine are also discussed in this communication.

ABSTRACT

Dysoxylum binectariferum Hook.f. (Meliaceae) is phylogenetically related to the Ayurvedic plant D. malabaricum. D. binectariferum has been identified as an enriched source of rohitukine, an alkaloid. Two synthetic flavones, flavopiridol (Alvocidib; L868275; HMR-1275; NSC 649890 of Sanofi-Aventis + NCI) and P-276-00 (Piramal) are advanced in clinical trials for the treatment of cáncer. These two molecules are considered as potent Cdk inhibitors. Cdk has been discovered as a key regulator of cell cycle. Therefore, Cdk inhibitors or modulators are of great interest to explore as novel therapeutic agents. These two molecules can be considered as rohitukine inspired drugs since their stuructural novelity originated from a compaign to develop a route of total synthesis of rohitukine. Recently a semisynthetic derivative of rohitukine; IIIM-290 has been emerged as potential preclinical cyclin-dependent kinase (Cdk) inhibitor. Moreover, phytochemical investigation of D. binectariferum yielded dysoline a new region-isomer of rohitukine. Dysoline seems to be a potent inflammatory and cytotoxic compound. Similarly, camptothecine and 9-methoxycamptothecine were isolated from Dysoxylum binectariferum for the first time by bioassay-guided fractionation. Present chapter is reviewing some original work which were carried out at CSIR-Indian Institute of Integrative Medicine, Jammu, India. The present studies focused on the phytochemistry of Dysoxylum binectariferum; dysoline, camptothecin and 9-methoxycamptothecin and medcinal chemistry and preclinical evaluation of IIIM-290 a semi-sythestic derivative of rohitukine.