eChapters

8.1. Origin and distribution

• Originated in South and South East Asia.

• Cultivated mostly from Pakistan to Papua New Guinea.

8.2. Economic importance

• In India and Sri Lanka, sheaf of betel leaves are traditionally offered as a mark of respect and auspicious beginnings like greeting elders at wedding ceremonies, celebrating the New Year, and offering payment to Ayurvedic Physicians and Astrologers (To whom money and/or areca nut placed on top of the sheaf of leaves are offered in thanks for blessings).

• Consumed in Asia as betel quid or paan with arecanut and/or tobacco.

• Used in cooking usually raw for its peppery taste.

• Medicinal use over a 300 years history in China.

Botanical Name : Piper betel
Family : Piperaceae
Origin : Malaysia.
Chromosome No. : 2n=2x=26 
Useable Part : Leaf 
    ·    Betelvine leaves have a strong pungent aromatic flovour and are widely used as masticatory. 

Scale insect: Lepidosaphes sp.: Diaspididae

The mature scale is elongated and oval in shape and dark grey in colour. They infest the stems, leaves and petioles, causing shriveled leaves due to sucking of the sap. The affected parts wither and dry up. In severe infestation leaves dry up and give sticky look and gradually the vine wilts.

1. Vein-Clearing/Yellow Vein Mosaic
Bhendi yellow vein mosaic virus
Diagnostic Symptoms
• Yellowing of the entire network of veins in the leaf blade is the characteristic symptom.
• In severe infections, the younger leaves turn yellow, become reduced in size and the plant is highly stunted.
• The veins of the leaves will be cleared by the virus and intervenal area becomes completely yellow or white.
• In a field, most of the plants may be diseased and the infection may start at any stage of plant growth.
• Infection restricts flowering and fruits, if formed, may be smaller and harder.
• The affected plants produce fruits with yellow or white colour and they are not fit for marketing (Fig.1).
• The virus is spread by whitefly.

Shoot and fruit borer: Earias vitella (=E.fabia), E. insulana: Noctuidae

This is a pest on cotton, bhendi, hibiscus, holly hock and other malvaceous vegetables. The damage is due to the larva that bores into tender terminal shoots in the vegetative stage and flower buds;  flowers and young fruits in the fruit formation stage.  The damaged shoot drops, wither and dry up.  The infested fruits have bore holes, are deformed and become unfit for consumption.  Bore holes are plugged with excreta.

Introduction

Antioxidants are substances, which retard or prevent deterioration, damage or destruction caused by oxidation. Fortunately, the body has an army of antioxidants for damage limitation. Antioxidant form an integral part of the nutraceutical market. During the last few years’ research has confirmed that many have the common disease and ailments of 21^st century (Coronary Vascular Disease, diabetes, cataracts, high blood pressure, infertilities, respiratory infection, and rheumatoid arthritis) are associated with tissue deficiency and/or low dietary levels of compounds called antioxidants. The oxygen is consumed in the body during metabolism by process called oxidation. During oxidation free radicals are generated. These free radicals at a molecular level burn everything they touch. Antioxidants are power full electron donors and react with free radicals which damage the biomolecules. The formed antioxidant radical is stable and non-reactive. Antioxidants are quite large in number and diverse in nature which oppose the process of oxidation largely by neutralizing free radicals at relatively small concentrations and also have the potential to inhibit the oxidant chain reactions.

Introduction

Khejri (Prosopis cineraria) grows in dry and arid regions of Arabia and in regions of India mainly Rajasthan, Haryana, Punjab, Gujarat, Western Uttar Pradesh and drier parts of Deccan. The tree is locally known as Jandi or Khejri (India), Jand (Pakistan) and Ghaf (Arabic). It is the State tree of Rajasthan, India. Prosopis cineraria are small to medium size evergreen thorny tree. It is also known as “wonder tree” and “king of desert” as all the parts of tree are useful. The importance of the medicinal value of this tree has been highlighted in our ancient literature. The bark of the tree has abortifacient and laxative properties and is also used as a remedy for rheumatism in the central provinces. The leaves are of high nutritive value and locally called ‘’Loong’’. Leaf paste of P. cineraria is applied on boils and blisters, including mouth ulcers in livestock. The smoke of the leaves is considered good for eye troubles. Leaf extracts of P. cineraria have been reported to show antibacterial, antihyperglycemic, antihyperlipidemic and antioxidative activities . Pods are brown to chocolate in colour on ripening, each containing several seeds embedded in sweet dry yellow pulp. P. cineraria pods are locally called ‘’sangar’’ or ‘’sangri’’. The pod is considered astringent in Punjab. Sangri pods are known to prevent protein and mineral deficiency. Cooked pods of Khejri are used as a functional food in Rajasthan, for the amelioration of numerous illnesses. Pods of khejri have been studied for various phytochemicals like alkaloids, saponins, tannins, flavanoids, flavanols and total phenolics. As this plant is found in water stress (or deficient area) so antioxidant potential of pods has also been investigated. Acetone and methanol extracts of Sangri pods are the most potent bioactive extracts. Nutritional analysis shows it as a good source of proteins and minerals like calcium, sodium and potassium. This study demonstrates that P. cineraria pods may be employed as nutraceutical food with rich nutrition, disease prevention and health promoting effects.

1. Introduction 
Among the innumerable gifts of nature, plants, animals and minerals have been the basis of treatment of human diseases. Generally it is either of prebiotic origin or originating from microbes, plants or animal sources. More likely they are the natural expression of the increase in complexity of organisms. Beside animals, plants are one of the most important sources of natural products. From the very beginning of their existence, man has acquainted himself with plants and used them in a variety of ways throughout the ages. In search of food and to cope successfully with human sufferings, ancient man began to distinguish those plants suitable for nutritional purposes from others with definitive therapeutic action. Some plants are widely used as food while others showed beneficial effects against various human sufferings such as injuries and diseases. This relationship has grown between plants and man, and many plants have come to be used as drugs. The use of plant as a drug can be divided broadly into five periods. The early period covers the Indian, Chinese, Sumerian, Egyptian and Assyrian civilizations followed by the Greco-Roman, Arabian, Medieval and modern periods (Buchnman, 1980). Among ancient civilisations, India has been known to be rich repository of medicinal plants as the forest in India is the principal repository of large number of medicinal and aromatic plants, which are largely collected as raw materials for manufacture of drugs and perfumery products. About 8,000 herbal remedies have been codified in Ayurveda. The Rigveda (5000 BC) has recorded 67 medicinal plants (Chopra et al., 1956), Yajurveda 81 species and Atharvaveda (4500-2500 BC) 290 species. Charak Samhita (700 BC) and Sushrut Samhita (200 BC) had described properties and uses of 1100 and 1270 species, respectively (Kirtikar & Basu, 1918; Baquar, 1989) in compounding of drugs and these are still used in the classical formulations, in the Ayurvedic system of medicine. Before the availability of synthetic drugs, medicinal plants were used as the most primary source of medicine and continue to provide the mankind with new remedies for many diseases. These were used in crude forms like expressed juice, powder, decoction or infusion. As the plant have specialized biochemical capabilities they are able to synthesize and accumulate a vast array of primary and secondary chemical constituents or phytochemicals (Ghazanfar, 1994). Phytochemicals are responsible for metabolic activities and used for defense purpose. They are produced by specific biochemical pathways, which occur inside the plant cells. Due to their physical characteristic and chemical properties these chemical constituents are highly beneficial to the mankind and most importantly in primary health care. The first chemical substance isolated from plants was benzoic acid (Figure 1) discovered in 1560. The potent pain-killer, morphine (Figure 1) was separated from the dried latex of Papaver somniferum L. (opium) by F. W Serturner (1783-1814). The bioactive molecule, ephedrine (Figure 1) is one of the most potent plant based medicine and was isolated from Ephedra sinica (Ma huang). It was used in the form of various salts to combat bronchial asthma (Tang & Eisenbrand, 1992). Reserpine (Figure 1) an indole alkaloid, obtained from the roots of Indian plant, Rauwolfia serpentina, was used to treat hypertension. Taxol (Figure 1) is a diterpenoid first isolated from the stem bark of pacific Yew, Taxus brevifolia and used against ovarian for the treatment of ovarian cancer.

ABSTRACT

In the present investigation on three different plant species from pollution and control area were carried act for biochemical analysis such as total chlorophyll content, leaf extract pH, relative water content and ascorbic acid test. The results indicate accordance to order of tolerance index of plant species in air pollution are Macrosolen cochinchinensis, Loranthus parasiticus and Viscum album. The changes in pollution tolerance index are biochemically induced due to the surrounding environmental conditions. Thus, it is observed that Macrosolen cochinchinensis is highly tolerant species among the selected three species. Thus, in future this plant species may play an important role in pollution indicator. APTI analysis show the pollution tolerating decreasing order for there species can be Macrosolen cochinchinensis> Loranthus parasiticus>Viscum album and as ascorbic acid result indicate that Macrosolen cochinchinensis show mostly defence mechanism and highly tolerance level against different kind of pollutant. Besides these three hemiparasite plant species have some valuable ethnomedicinal important. In India tribal people used leaf and stem bark in curing abortion, miscarriage, vaginal bleeding during pregnancy, treat circulatory and respiratory system problems.

ABSTRACT

Brinjal is an important solanaceous vegetable, which holds coveted position among the different vegetables. Developing hybrids with high yield and resistance to borer would be highly beneficial. The biochemical parameters are used to study the mechanism of resistance to pests and diseases. With this aim, the biochemical constituents were estimated in five intervarietal hybrids and their parents with susceptible variety. The study on biochemical basis of resistance to shoot and fruit borer showed that among the parents of intervarietal crosses, EP 65 had the highest level of peroxidase, polyphenol oxidase and total phenol content in both shoot and fruit and it also recorded high content of solasodine next to APAU Bagmathi. Among the hybrids, the cross EP 65 x Pusa Uttam had the highest level of peroxidase and polyphenol oxidase activity in both shoot and fruit and high level of total phenols and solasodine content next to EP 104 x APAU Bagmathi . The susceptible check (CO 2) registered the lowest value for all the biochemical constituents .The study revealed that the genotypes with high or moderate level of these biochemical constituents suffered less for shoot and fruit borer infestation.

The native gel electrophoretic study showed that for peroxidase enzyme, the intervarietal hybrids EP 65 x Pusa Uttam, EP 12 x MDU 1 and EP 104 x APAU Bagmathi and the susceptible variety CO 2 did not show difference in the induction of isoform (PO-1) and all were single and thick. For polyphenol oxidase (PPO), the intervarietal hybrids and the variety CO 2 showed single and strong band induction

The evaluation of genotypes based on biochemical constituents and isozyme studies revealed the superiority of the parents EP 65, APAU Bagmathi and MDU 1and the hybrids EP 65 x Pusa Uttam, EP 12 x MDU 1 and EP 104 x APAU Bagmathi. The identified hybrids with high yield and resistance to shoot and fruit borer could be exploited for commercial cultivation.

ABSTRACT

Astudy was undertaken at Department of Soil Science & Agrl. Chemistry, College of Horticulture, Vellanikkara to standardize different amelioration techniques to prepare enriched coirpith compost. Twelve treatments were tried using different combinations of coirpith, Pleurotus spp., Schizophyllus spp., urea, rock phosphate, cowdung slurry, mushroom waste, goat manure, glyricidia and water hyacinth. The results of the various enrichment techniques revealed that the temperature in the compost in general, increased to a peak value of 47oC on the 22nd day of composting and later decreased to a stabilized value. The observed temperature of 40-50 °C found during the thermophilic stage is essential for the rapid degradation of lignocellulose, as the thermophilic microfungi and actinomycetes involved in this process thrive at that temperature. Though the pH show a hike at the initial stage of composting, it decreased and stabilized at the later stage of composting which is conducive for growth and proliferation of microbes. The treatment coirpith with Pleurotus spp., glyricidia +20% cowdung slurry and goat manure was found to be ideal for compost preparation.

ABSTRACT

Detection of disease tolerance with enhanced defense mechanism is a practical approach in crop variety improvement programme. Toxins being responsible for pathogenicity and virulence of the fungi, regenerating plants from toxin treated calli is an effective and easy approach. The response of tomato calli to the crude toxins of the foliar pathogens viz., Alternaria solani and Septoria lycopersici was studied and the changes in phenol metabolism due to toxin treatment were analysed. Crude toxins of A. solani and S. lycopersici extracted under optimum conditions were serially diluted and tomato calli were treated with different dilutions of the toxins to find out the optimum dilution at which minimum survival percentage was obtained, based on a 1-4 score. Minimum score of 1 was obtained at 1:10 dilution for A. solani and at 1:20 for S. lycopersici.

The calli of resistant (LE-625) and susceptible (CO-3) tomato varieties were treated with desired dilutions of the toxins of A. solani and S. lycopersici. Total phenolics and ortho- dihydroxy (OD) phenolics were estimated at 24 h interval to study the extent of necrosis on resistant and susceptible calli due to toxin treatment. The total and OD-phenol contents of calli of susceptible cultivar (CO-3) progressively decreased with increase in time interval consequent to the treatment of toxins of A. solani and S. lycopersici. In the resistant calli of LE-625, an initial reduction of total phenol and OD-phenol content was observed following the application of the toxins of A. solani and S. lycopersici at 24 hours after treatment. Later with lapse of time, the total phenol content and OD-phenol contents progressively increased reaching a maximum at 120 h after treatment. The results clearly indicated the enhancement of biochemical defense in the resistant calli and a fall in defense mechanism in the susceptible calli due to toxin treatment. Such techniques are useful in the preliminary screening of resistant genotypes in crops like tomato.

Anacardium occidentale is a medium-sized tree, spreading, and evergreen, much branched; grows to a height of 12 m. When grown on lateritic, gravelly, coastal sandy areas, it rarely exceeds 6 m and develops a spreading habit and globose shape with crown diameter to 12 m but, if grown inland on loams; it reaches 15 m tall and is much branched, with a smaller (4-6 m) crown diameter. The root system of a mature A. occidentale, when grown from the seed, consists of a very prominent taproot and a well developed and extensive network of lateral and sinker roots. Leaves simple, alternate, coriaceous, glabrous, obovate, rounded at ends, 10-18 x 8-15 cm, with short petiole, pale green or reddish when young and dark green at mature. Rai and Vatsala (1985) studied the dermal morphology and stomatal ontogeny of Spondias pinnata Kurz., Lannea coromandelica (Houtt.) Merr., Anacardium occidentale L., Holigarna arnottiana Hook. F. and Mangifera indica L. are studied. The cuticle is thick in H. arnottiana, A. occidentale and M. indica, but is thin in S. pinnata and L. coromandelica. Based on the shape of epidermal cells, the five species are categorised into three: cells in both upper and lower epidermis sinuous (A. occidentale, H. arnottiana, S. pinnata); upper sinuous and lower polygonal (M. indica) and upper polygonal and lower sinuous (L. coromandelica). Stomatal ontogeny aperigenous (L. coromandelica), paramesoperigenous (A. occidentale) and anisomesogenous (M. indica, H. arnottiana). S. pinnata is peculiar in that it possesses three different types of stomata, aperigenous, hemiparamesoperigenous and paramesoperigenous; whereas, in all the other four species, only one type of stomatal ontogenesis exists. Except S. pinnata, all the other species possess epidermal appendages. Unicellular trichomes occur in A. occidentale and H. arnottiana, and multicellular glandular trichomes in A. occidentale, M. indica and L. coromandelica. The multicellular hairs are found in A. occidentale in specialized pockets in two rows on either side of the major veins - a unique feature which is reported for the first time.

The term biodiversity is abbreviated from two different terms ‘biological’ and ‘diversity’. Biodiversity is also called biological diversity. The term biological diversity was first coined by wildlife scientist and conservationist Raymond F. Dasmann in 1968. The same term i.e. natural heritage was used in the United State. Geneticists describe it as the diversity of genes and organisms. Biologists define biodiversity as the sum of genes, species and ecosystems of a region.

Biodiversity is determined by the wholesome number of different species in a particular area. It helps to strengthen the stability of an ecosystem and leads to a healthy biosphere. Existence of biodiversity is crucial for the functioning of the ecosystem and is the foundation of life on earth as it generates products and services for the existence of all living organisms on the earth.

1. Introduction

Total population of India is estimated to cross 1.64 billion in 2050. Increasing population, decreasing economic status of Farmer, improper diet and nutrition, fixed farm land, degradation of cultivable land and malnutrition in Indian population is major key problem in 2 to 3 decades. More than 600000 children below 5 year age die due to improper nutrition each year in developing countries (White and Broadley, 1998). All the data provide a scary image about future. Almost 60% of the malnutrition children are iron deficient. Iron deficiency is very common among the girls; 30% each are deficient in zinc and iodine. The incomplete diet and malnutrition can lead to several disorder and degrade the human health (Shuman, 1998).

The Distributed Information Sub Center at Kerala Agricultural University, Thrissur was set up in May 1995 as the 23^rd center of the BTISNet of DBT, Government of India. The area of specilisation of the Centre is Agricultural Biotech- nology. The Centre passed through several phases involving Internet connectivity through the ordinary C band connectivity, then the Ku band connectivity etc. Now the commu- nication facilities of the Centre have been strengthened by procuring an ISDN Internet connection and a connection through ASIANET. The Center caters to the needs of the students (UG, M Sc & Ph D) and faculty of all departments of the Colleges in the main campus as well as of the other nearby academic and research centers of the University. Over the years, DISC has come to occupy a position in the KAU in the area of Infor- mation Technology , information sources and services based on Internet, CD-ROMs etc. The Centre has procured databases in the field of Biotechnology and developed several databases in the field of Agriculture and related fields. The center regularly conducts training programmes in Bioinformatics. The research activities in the field of Bioinformatics have been enhanced. The Centre has now been upgraded to DIC to facilitate intensive research on Bioinformatics in an effective manner. During the year 2004, the Centre has also started offering a course on Bioinformatics for the M.Sc Biotechnology students of the College of Horticulture.

Biotechnology research in KAU spread over various research stations and cam- puses includes Plant Biotechnology, Animal Biotechnology and Fisheries Biotechnol- ogy. Thrust areas for Plant Biotechnology include developing in vitro protocols for mass multiplication of elite types of fruit plants, ornamentals, medicinal plants, spices, plantation crops and forest species. The other thrust areas include checking clonal stability and genetic fidelity of in vitro derived plants using molecular markers, DNA finger printing and characterisation of germplasm with molecular markers, imparting biotic and abiotic stress tolerance in otherwise accepted crop varieties, genetic trans- formation for imparting/ improving expression of desired traits, in vitro germplasm conservation, enhancement of secondary metabolites production etc.

The crops identified for biotechnology research at KAU include spice crops like black pepper, ginger, turmeric, vanilla, cardamom; plantation crops like cashew, coco- nut and medicinal & aromatic plants such as Kaempferia, Trichopus, Gymnema, Holostemma and Coscinium. For improvement of such crops through molecular breed- ing, it is highly essential to identify/design specific probes/primers, micro-satellites, etc. By combining the research results generated at the Biotechnology Centre and informa- tion retrieved from the Bioinformatics Centre, suitable probes, primers are being de- signed for characterization and improvement of these unique crops through molecular breeding.

Fruits with their delightful colours, flavours and tastes are nature’s wonderful gift to mankind. Apart from being the most nutritious food in the form of easily digestible simple sugars, vitamins, minerals and many phyto-nutrients (Plant derived micronutrients), they provide unique health benefits due to their antioxidant properties and valuable fibre. Antioxidants help protect the human body from oxidant stress, diseases, and cancers, and also boost the immunity of the body thus developing the capacity to fight against various types of diseases. The insoluble dietary fiber in fruits is known to provide protection from conditions like chronic constipation, hemorrhoids, colon cancer, irritable bowel syndrome, and rectal fissures etc. Due to their unique nutrition-profile the importance of fruits in our diet cannot be over emphasised.

Insects are a major limiting factor in fruit production. The infestation of insects pests on fruit trees in orchards adversely affects the quality and yield of fruits. The size, shape, taste, attractiveness and palatability of fruit deteroriates, due to which the market price is reduced and the growers incur losses. Fruit growers resort to use of chemical pesticides under such conditions. However many reports suggest that although the global production and use of pesticides in agriculture is increasing every year, but the damage due to insects has not decreased rather it has also increased, indicating that the use of pesticides is not the panacea remedy for all insect related problems. Many scientists are of the opinion that over-reliance on the use of synthetic pesticides in crop protection programs around the world has resulted in disturbances to the environment, pest resurgence, pest resistance to pesticides, and lethal and sub-lethal effects on non target organisms especially beneficial flora and fauna, including humans. Farmers spend thousands of rupees on pesticides. The residues of pesticides in food and fruits is yet another cause of concern. Fruits which are considered to be wonderful for health are often laden with unacceptable levels of pesticide residues nulling their beneficial properties. Pesticides have been linked to many diseases especially cancer. All these issues related with use of pesticides has triggered rethinking on the methods of control.The recent examples of the results of indiscriminate pesticides use , consequent crop failures and suicide by farmers now calls for introspection in this matter.

Biological control or biocontrol is a method of controlling pests such as insects, mites, weeds and plant diseases using other organisms. It relies on predation, parasitism, herbivory, or other natural mechanisms, but typically also involves an active human management role. It can be an important component of integrated pest management (IPM) programs.

1.0 INTRODUCTION

“Biological control is the reduction of the amount of inoculum or disease producing activity of a pathogen  or parasite  in its active or dormant state, by one or more organisms, accomplished naturally or through manipulation of the environment, host or antagonist or by mass introduction of one or more antagonists ” (Baker & Cook 1974). The biological world is a vast interacting network of living organisms in a state of dynamic equilibrium, reflecting changes in their physical environment and their relations to each other. Organisms exhibit many type of interactions in the process of achieving biological balance.  An organism may exert no effect on another or it may stimulate or inhibit the growth or development of the associate, it may stimulate or inhibit the formation of resting spores of the associate or it may enforce dormancy or cause lysis of the associate (Cooke & Baker, 1983).

1.0 INTRODUCTION

Infestation by insect pests is a major factor responsible for the low productivity of spice crops in India. Spice crops such as black pepper (Piper nigrum L.), cardamom (Elettaria cardamomum Maton), ginger (Zingiber officinale Roscoe) and turmeric (Curcuma longa L.) are infested by many species of insect pests, some of which cause severe crop losses. Adoption of integrated pest management (IPM) schedules in which biological control forms a major component is more relevant in these crops since excessive use of insecticides could lead to pesticide residues in the produce and other ecological hazards. Spices are high-value and export-oriented in nature and pesticide residues are the most important non-tariff barriers on trade in these produces. There has been a renewed interest during the past decade in developing environment–friendly crop management schedules in sustainable agriculture including spice crops, reflecting the increasing concern over pesticide misuse.