eChapters

I. INTRODUCTION

The fruits and vegetables we eat and flowers and ornamentals we enjoy are biological entities. They breath, produce heat, perspire, are easily damaged, get diseases and finally die. These carry on processes characteristics of all living things before or after harvest to perform the metabolic activities:

1. to maintain cellular organization
2. to transport metabolites around the tissue and
3. to maintain membrane permeability.

I. INTRODUCTION
The fruits and vegetables we eat and flowers and ornamentals we enjoy are biological entities. They breath, produce heat, perspire, are easily damaged, get diseases and finally die. These carry on processes characteristics of all living things before or after harvest to perform the metabolic activities:
1. to maintain cellular organization
2. to transport metabolites around the tissue and
3. to maintain membrane permeability.
The job of postharvest technologist is to understand life in fruits, vegetables and flowers and prolong their life as much as possible. The post harvest life of produce depends on the rate at which they use up their stored food reserves and their rate of water loss. The sugar and starches (carbohydrate derivatives) are stored in various parts of plants through a process known as photosynthesis occurring in growing plants and are used later to provide energy for further growth, development and to prolong post-harvest life. The process of photosynthesis in growing plants and respiration in harvested produce can be seen in Fig. 2.1. The released energy through process known as respiration occurs in plants before and after harvest. Thus, principal normal physiological processes leading to aging are respiration and transpiration.

Ethnopharmacology and Traditional medines use natural resources as medicines. They have developed the knowledge of this through the generations of traditions and experiences. Therefore the protection of this knowledge and protection of natural resources both remain critical. Bioprospecting involves sustainable, ethical and legal processes to access natural resources and use them judicially so that the business needs do not pose any threat to nature and to people.

One important consideration is assuring the preservation of traditional knowledge, particularly oral knowledge, within communities, for the potential benefit of others. Developing mechanisms to document traditional knowledge, such as national inventories, can be beneficial in identifying cases where conservation or cultivation is needed, based on an understanding of existing natural resources used for medicinal purposes. Decisions about access and ownership of information can also be made (including those relating to intellectual property rights, where appropriate) once methods have been found to collect and preserve traditional knowledge. On the other hand, the livelihood of traditional healers may depend on maintaining the secrecy of their healing methods - one argument for consulting with traditional healers as part of the process of planning and executing the collection, storage and testing of traditional medicines.

Extending some form of intellectual property protection to traditional knowledge, of which traditional medical knowledge is a significant part, is being actively debated in WIPO and other international forums. Existing intellectual property regimes can offer protection for purified compounds (produced by scientists and companies) isolated from a natural medicine. But traditional medicines, whose method of action may not be well understood, and which often consist of mixtures of different active substances, may not meet criteria for patentability. The principal impetus for international debate is a desire to compensate the holders of traditional knowledge appropriately for the use of their knowledge by others, or to prevent so-called “biopiracy” where knowledge or genetic materials are used without the consent of the holder. China and Kenya are examples of countries that have modified their laws in an effort to accommodate traditional medicine. It is not intended that the Commission should enter into this debate, except in so far as aspects of it may be relevant to its terms of reference, in particular stimulating innovation in traditional medicine with the purpose of expanding access to affordable medicines.

1. INTRODUCTION

Chemical pesticides are designed to control or eliminate pests such as insects, rodents, weeds, bacteria, and fungus. Although pesticides have played a significant role in increasing food production and controlling various diseases in the agriculture system but indiscriminate use of pesticides has resulted in contamination of soil, water bodies’ etc. raising deep concern about food safety and human health. Excessive use of these harmful chemicals affects kidneys, developing fetus, and liver immuno-suppression and increased incidence of breast cancer. Some of these pesticides are also reported as mutagenic. Other health related problems such as impaired memory and concentration, severe depression, headache, etc have also been reported.

Through field survey (Kadian et al., 2010) it has been found that chlorpyrifos is a widely used pesticide in household application as well as in horticulture crop in National capital region Delhi. This tempted us to understand the nature of chlorpyrifos (CPF) and its metabolites and assess the suitability of available techniques for detoxification of contaminated soil.

Introduction

Several hazardous chemicals, microbes, and techniques like recombinant DNA technology are used for research and development work in research Institutes and universities. Therefore, the researchers, technical, helping and supporting staffs as well as the laboratory outputs must be kept free from contaminations. Thus, biosafety is necessary to prevent unintentional exposure/ release of hazardous chemicals, microbes/pathogens, and toxins to the laboratory staff and environment. Following the biosafety guidelines and ethical considerations are essential to maintain safety standards for the researchers as well as the environment. This chapter deals with various biosafety measures and ethical issues to be strictly followed while working in a research laboratory. The required safety level needs to be determined as per the chemical/biological materials are used in the laboratory to keep the researcher/environment safe for sustainable research and developmental activities.

Biosafety

The term biosafety is used to describe the procedure and policies needed to ensure environmental and personal safety. Biosafety refers to the containment principles, technologies and practices that are implemented to prevent unintentional exposure to pathogens, toxins, or their accidental release into the environment. A fundamental objective of any biosafety program is the containment of potentially harmful chemical/biological agents as precautionary safety measures.

Why Biosafety?

With the increasing number of countries adopting molecular biology tools and techniques in life science research and development activities, especially in the areas of agriculture and medicine, the biosafety issues are gaining importance to ensure biological safety for the public and the environment. Conducting research in safe and sustainable manner is not only a personal requirement but essential collective efforts to ensure biological safety for a clean and safe environment. This requires rules, regulations, monitoring bodies, and awareness among the workers as well as the public. Therefore, biosafety per se is an integral part of laboratory research and requires awareness among the researchers so that biological safety can be well taken care from the grassroot level.

. Introduction

A primary issue for modern horticulture is facing two contradictory objectives, such as the need to produce food for the increasing world population and to minimize damage to the environment, which can in turn negatively impact horticulture. Fertilizer and chemical use and manufacture are hazardous to the environment and humans. As a result, new and effective environmentally friendly compounds are required. Plant biostimulants, which are harmless for humans and the environment, are one answer. They are particularly useful for lowering pesticides in agriculture. Plant biostimulants have become popular in farming and cultivation in recent years.

ABSTRACT

The use of engineered nano-materials has been increased as a result of their positive impact on many sectors of the economy including agriculture. Silver nanoparticles (AgNPs) are now used to enhance seed germination, plant growth and as anti-microbial agents to control plant diseases. This study investigated the synthesis of silver nanoparticles using the aqueous solution of three medicinally important plants (such as Spondius mombin L., Stachytarpheta jamaicensis (L.) Vahl and Syzygium samarangense (Blume) Merr & Perry leaf extract in room temperature (350C). AgNPs present in the leaf confirmed by colour change, UV-VIS spectrum analysis, average size of AgNPs confirmed by PAS. FTIR measurement were carried out to identify the other possible biomolecule groups such as alkenes, esters, phenols, alcohols and aromatic. Leaf extract AgNPs were shown significantly higher antimicrobial activities against four species of bacteria. This findings suggest that the seed germination percentage, relative seed germination rate, relative shoot and root growth and germination index of the tested plant depends upon concentrate gradient of AgNPs.

ABSTRACT

Recognizing the need to apply plant tissue culture tools for generating planting materials of important spice crops especially vanilla, the Spices Board started Biotech Production Unit at its head quarters building at Kochi with an annual production potential of 8 lakhs plantlets. Complete tissue culture protocols were developed and scaled up for large scale production of vanilla and other spices viz. large cardamom, small cardamom and tree spices. The laboratory is equipped with all modern facilities and is established in an area of about 2000 sft. Department of Biotechnology, Govt. of India also participated in the venture by sanctioning a project entitled “Regional centre for large scale production of tissue culture plantlets of vanilla and other spices” with a financial out lay of Rs 95 lakhs equally shared by DBT, New Delhi and Spices Board, Cochin.

At present there is a stock of over 3.00 lakh vanilla cultures in the lab and already 1.25 lakh plant lets were sent for hardening. The Spices Board, in coll aboration with “Kudumbashree”, a poverty eradication mission of Govt. of Kerala, extend short term training in tissue culture techniques to undergraduates from ‘Below Poverty Line’ (BPL) families and engage them as long term trainees in the Biotech Production Unit. The Board also coordinates and extends support to Kudumbashree members for, establishing and maintaining hardening facilities for TC plantlets thus providing means for empowering women from BPL families.

Role of Biotechnological Approaches

In our fore-going chapters, we have studied variety of plant products such as alkaloids, glycosides, volatile or essential oils, gums and mucilage and oleoresins with varied role to play to the plants as well as to human.Knowledge of plants and knowledge of healing have been closely linked. It is well established that industries have many direct and indirect effects on human population. Increased stress is most evident at the same time health awareness goes parallel in the recent times. Most of the natural plant products are compounds biosynthetically derived from primary metabolites such as amino acids, carbohydrate metabolic intermediates and fatty acids. These secondary metabolites are the major sources of pharmaceuticals, food additives, fragrance and pesticides.

Application of biotechnology know how helps to synthesize the novel molecules and also could be beneficial to enhance the production of secondary metabolites. Classical definition of biotechnology states that it is “The use of living organisms or their products to enhance our lives and our environment.” The word Pharming is commonly use to describe genetically engineered plants or livestock to produce medically useful products –predominantly protein drugs. Plants or animals are genetically engineered to contain genes capable of producing a biologic or drug compound. Therapeutic proteins are designed to treat illness includes serum proteins, cytokines, growth regulators, anti coagulants, antbiotics, lysosomal enzymes and plant secondary metabolites.

Introduction

The horticulture sector has been a driving force in stimulating a healthy growth trend in Indian agriculture. This sector contributes around 28 per cent of the GDP from about 13.08 per cent of the area and 37 percent of the total exports of the agricultural commodities (Rather et al., 2013)  India is currently producing 257.2 million tonnes of horticulture produce from an area of 23 million ha. Over the last decade, the area under horticulture grew by about 3.8% per annum but production rose by 7.6% per annum. The higher growth rate in horticulture was brought about by improvement in productivity of horticulture crops, which increased by about 28% between 2001-02 and 2011-12. (Anonymous, 2012)

The main purpose of herbal drug technology is to convert botanical materials into medicines with a focus on standardization and quality control with proper integration of modern scientific techniques and traditional knowledge. The use of chromatographic techniques and marker compounds to standardize botanical preparations has limitations because of their variable sources and chemical complexity. DNA-based molecular markers have utility in fields like taxonomy, physiology, embryology, genetics etc. DNA based techniques have widely been used for authentication of plant species of medicinal importance. Pharmacognosy mainly addresses the quality related issues using routine botanical and organoleptic parameters of crude drugs, and chemoprofiling assisted characterization with chromatographic and spectroscopic techniques. The new pharmacognosy includes all the aspects of drug development and discovery where the biotechnology driven applications play an important role. Current focus on chemotype-driven fingerprinting and related techniques require integration with genotype-driven molecular techniques so that an optimal characterization of botanical materials is possible. This review provides a brief account of various DNA based technologies that are useful in genotyping and quick identification of botanicals with suitable examples.

Abstract

Tea [Camellia sinensis (L.) O. Kuntze], of family Theaceae, is an important cash crop  worldwide. It is an evergreen shrub cultivated in humid and sub-humid tropical, sub-tropical, and temperate regions of the world, and grows mainly on acidic soils. Due to limitation of expansion of tea cultivation in new land, import of low cost high quality tea, increasing vertical productivity is the best option of Indian tea Industry. The primary need of the tea industry today is to develop high yielding clones with better quality and stress tolerance potential. Conventional tea research has contributed appreciably to the genetic improvement of tea over the past several decades, but it is time-consuming and labor intensive.

It is difficult to define ‘stress’ precisely. In engineering and the physical sciences, it is usually defined as the force applied per unit area. But as far as in biology, stress it is usually described more loosely as any factor that disturbs normal functioning of an organism. Any environmental factor potentially unfavorable to plant is termed as stress.

The effect of stress on plant condition is called strain. Stress is the action, whereas strain is the reaction. Stress may be defined as external conditions that adversely affect growth, development and productivity. Stress may further classified as:

• Zero stress: This is the level of exposure of plants to environmental factors that leads to neither injury nor reduction in growth and yield of crops.
• Stress escape: Capacity of the plant to complete its life cycle during wet season before onset of stress.

English name : Bishop’s weed, Ajowan
Botanical name : Trachyspermumammi (L.) Sprague
Family : Apiaceae
Chromosome number : 2n = 18
Centre of origin : Egypt
Ajowan is an annual herbaceous plant grown for its herb and greyish brown fruits (seeds) which constitute the spice. The trade name ajowan or ajwain
is based on the Indian name which is derived for adarjawan. It is known as ‘Bishop’s weed’ in English. Ajowan is grown for its seeds (fruits) the composition of which is as follows; moisture 8.9%, protein 15.4%, fat (ether extract) 18.1%, crude fibre 11.9%, carbohydrates 38.6%, mineral matter (total ash) 7.1%, calcium 1.42%, phosphorus 0.30%, iron 14.6 mg/100 g, vitamins (per 100 g) thiamine 0.21 mg, riboflavin 0.28 mg, nicotinic acid 2.1 mg, carotene 71 mg. Its calorific value is 379.

Anthracnose
Diagnostic Symptoms
• Pathogen attacks all aerial parts of plants and at any stage of their growth.
• Symptoms are circular, black, sunken spots with dark center and bright red orange margins on leaves and pods.
• In severe infections, the affected parts wither off.
• Seedlings get blighted due to infection soon after seed germination.
• Infected pods have discolored seeds (Fig.3).
Etiology
Scientific Classification
Kingdom : Fungi
Division : Eumycota
Sub- division : Deuteromycotina

19.1. Origin and distribution

• Native to Eurasia and introduced in America, Australia and South Africa.

• Grown throughout dry parts of India

19.2. Economic importance

• Whole plant - Useful for rheumatalgia, swellings, cough, asthma, bronchitis, wounds, ulcers, flatulence, dyspepsia, hepatomegaly, otalgia, hiccough, opthalmopathy, vomiting

• Used in cardiopathy, leprosy, skin diseases, fever, splenomegaly, emollient

• As an antiseptic, anti-inflammatory, expectorant, anodyne, vulnerary, digestive, laxative, diuretic, cardiotonic, depurative, diaphoretic, febrifuge, rejuvenating, sedative, alterant and tonic.

19.3. Climate

• Well in tropical and subtropical climate

Black pepper (Piper nigrum L.), famous as “Black Gold” and also known the “King of Spices” is the most important agricultural commodities of commerce and trade in India. It is the most important, most popular and most widely used spice in the World. Of the total spices traded internationally, black pepper accounts for 34 per cent. South West India is the traditional home of this important spice, particularly the Malabar Coast. Black pepper is also grown in tropical zones such as the Asia Pacific Region, mainly India, Indonesia, Malaysia, Sri Lanka, Thailand, China, Vietnam and Cambodia. Outside the Asia Pacific region, the crop is distributed in Brazil, Mexico, Guatemala, etc., totaling about 26 countries. It is grown successfully between 20°N and 20°S latitude and from mean sea level to 1500 meters above sea level. World’s black pepper production varies in the range of 2.7 to 3.35 tonnes annually. The major production share is contributed by Vietnam with an annual production of 80,000 tonnes. About 85 per cent of the World trade of pepper is determined by the largest pepper producing countries in the International Pepper Community (IPC). World export fluctuates around 1.5–2.5 lakhs tonnes per annum. Global black pepper supply has shown a drastic increase over last ten years except in 2008–09. Vietnam’s sudden increase in production has resulted in the massive global out put of 3–3.5 lakhs metric tonnes from 1.9–2.0 lakhs metric tonnes in late nineties. 

English name : Black pepper
Botanical name : Piper nigrum L.
Family : Piperaceae
Chromosome number : 2n = 52
Centre of origin : Western Ghats, India
Black of the commerce is the dried mature fruit of the perennial climbing vine of Piper nigrum L. It is one of the oldest and most significant spices in the world. Black pepper refers to the whole dried fruit, which is still unripe, while white pepper is made from the fruit after the mesocarp has been removed. Among all spice crops, pepper holds the highest share in foreign exchange, earning it the title of the “King of Spices.” An analysis of various types of black pepper from Kerala, Karnataka, Tamil Nadu and Assam revealed the following ranges of values: moisture content (8.7-14.1%), total nitrogen (1.55-2.60%), nitrogen in non-volatile ether extract (2.70-4.22%), volatile ether extract (3.0-4.2%), non-volatile ether extract (3.9-11.5%), alcohol extract (4.4-12.0%), starch (28-49%), crude fibre (8.7- 18.0%), crude piperine (2.8-9%), piperine (spectrophotometrically) (1.7- 7.4%), total ash (3.9-5.7%), and acid-insoluble ash (0.03-0.55%). Starch is the principal constituent of pepper, making up 34.8% of black pepper, 56.5% of white pepper, and 63.2% of decorticated white pepper. Pepper starch consists of small, polygonal granules similar to rice but much smaller. The alkaloid piperine (melting point: 29-30°C) is the primary compound responsible for black pepper’s characteristic biting taste. Other pungent alkaloids present in smaller quantities include chavicine, piperidine, and piperetine. 

1.0 INTRODUCTION

Black pepper popularly known as ‘King of spices’ or ‘Black gold’ is one of the important and earliest known spices produced and exported from India. Its original home is the dense evergreen forests of Western Ghats in south India. The flavour and fragrance of this oriental spice had a magic spell in human civilization and culture since very ancient days. Expeditions in search of pepper led to the discovery of new trade routes.

Some species of turmeric have been documented from China, Australia, and the South Pacific, despite the fact that turmeric has been farmed all throughout the tropics. Turmeric along with the 110 species is found growing in Tropical Asia, with India, Myanmar, and Thailand having the most diversity. Black turmeric (Curcuma caesia), along with Curcuma aromatica, Curcuma amada, Curcuma aeruginosa, Curcuma longa, and Curcuma zanthorrizha, is one of the economically important species native to India. Curcuma caesia (black turmeric/ black zedoary) is a critically endangered perennial herb in the Zingiberaceae family native to India. Traditional healers employ the Curcuma genus to treat a variety of diseases in ethno medical traditions, but Curcuma caesia Roxb. is a relatively unknown and unexplored medication. In the Indian subcontinent, black turmeric is frequently used as a spice, food preservative, and colouring ingredient. Black turmeric is the most recent spice to be chemically studied, with a few papers describing 100 or more secondary metabolites from this plant, and it will continue to be a source of new bioactive chemicals.

Distribution

Black turmeric is a native of Northeastern and Central India, where it has long been used in traditional rituals and medicinal treatments. Within an altitudinal range of 200–1000 m, the species is found in moist deciduous woods, primarily in Bengal, North East, and Central India. It develops as a woodland ground cover in subtropical to temperate climates.