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Worms and other intestinal parasites are found in every part of the world. They are more common in tropical and sub tropical areas and are more common during the rainy season. Most of these worms and parasites enter in the human body either through the mouth or through the skin of the feet.

Round worms, are the giant worms present in the intestines that cause the disease kwon as ascariasis. It is found in almost all the countries. These worms are off-white in colour, are long and thick and are most frequently seen in children. Eggs produced by them are passed in stools. When children play in the soil contaminated with the eggs, particularly children living under unhygienic conditions easily transfer them to the mouth via dirty fingers.

Roundworm eggs may remain alive in the soil for many months. There is always greater danger of contamination where human wastes are used in fertilizing vegetables. Great care must be taken to be sure all foods are thoroughly cleansed and sterilized by cooking before being used.

Quality Control is very important in biofertilizer production, which determines the efficiency of microbial strains. Generally, the quality control based on BIS are voluntary in nature. However, some of the biofertilizers are brought under the ambit of Fertilizer Control Order 1985 (FCO) during 2006 (Yadav and Chandra, 2014). At present, biofertilizers are under the FCO with specific quality standards. Biofertilizer production and its sales can be regulated and is a mandatory requirement for registration of manufacturing unit with the State Fertilizer Controller (who is generally the Commissioner or Director of Agriculture Department) under the statutory provision of FCO. Moreover, some officers of the Agriculture Department have been declared as Fertilizer Inspectors at the district level, who are authorized to inspect production and storage facilities and draw samples for quality analysis. National Centre of Organic Farming (NCOF), Ghaziabad under the Govt. of India and its six Regional Centre’s located at Bhubaneswar, Bangalore, Jabalpur, Nagpur, Hissar and Imphal have been declared as testing laboratories. Under the provisions of the FCO act, State Governments can also develop their own quality control laboratories and notify them under the FCO (1985). Peat, lignite, peat soil, humus, wood charcoal or similar material favouring the growth of microbial strain could also be used. The FCO specifications for different biofertilizers are presented below:

Jaundice is a disease marked by a yellow discoloration in the skin, or in the whites of the eyes. This is due to excessive amounts of bile in the blood stream. Jaundice may arise from a number of different causes. Some types of jaundice arise from conditions outside the liver. Also in some cases the patient may have jaundice in a sub clinical form. That is, the bile pigment may be elevated but not sufficiently to actually discolour the skin.

Normally there is a certain rate at which the red blood cell are broken down and destroyed in the body. The average life of a red cell is about four months. But certain disease conditions may cause an excessive breakdown of red cells and this increases the bile pigment in the blood. Such a rapid breakdown of cells may be seen in haemolytic anaemia, sickle cell anaemia, pernicious anaemia, jaundice of the newborn child, as well as in malaria, pneumonia, yellow fever and certain bacterial infections of the blood.

Obstructive jaundice means that the bile is backing up into the blood stream because of some obstruction involving the larger bile ducts. The normal liver produces one pint of bile every twenty-four hours. This is transported to the gall bladder where it is concentrated and becomes green, or else passes directly to the small intestine where it acts as a lubricant. Bile is also necessary for the absorption of fats and for its laxative effect in the large intestine.

23.1. Aim

To determine the total soluble solids in fresh and processed fruit and vegetable products.

23.2. Theory and principle

Total soluble solids (TSS) are determined by means of brix which measures the specific gravity or by using hand refractometer which measures refractive index. Brix is a measure of soluble solids only in case of pure sucrose solutions. Generally, the fruit juices contain more sugar than any other soluble constituent. Hence, brix provides a useful guide of soluble solids or sugar contents. Brix hydrometer is used to determine the percentage of brix in syrup while Abbe refractometer gives refractive index as well as degree brix. The Refractive index of the test solution is measured at 20°C using a refractometer and the soluble solid content is determined by the use of tables correlating refractive index with soluble solids. Generally, hand refractometer is used for determining TSS in food samples in fruit processing units (Table 3.1).

The common stones found in the kidneys are of calcium oxalate, uric acid, urates, triple phosphates or a combination of these.

The patients are often males between 30 to 50 years.

Horticulture includes variety and diversity of crops. Thus, it can be divided into several branches depending upon the crops it deals with. Following are the branches of horticulture.

1. Pomology: The term pomology is a combination of two Latin words pome i.e. fruit and logos i.e. science. Recently it has been designated as ‘Fruit Science’. It deals with the study of fruit crops. It includes all aspects of fruit crops e.g. importance, uses, origin and distribution, area and production, soil and climate requirement, botany, nursery raising, cultivation practices, plant protection measures, stage and methods of harvesting, post-harvest handling, marketing, export, import, etc. e.g. mango, banana, citrus, grape, apple, peach, pomegranate, fig, etc.
2. Olericulture: The term olericulture is also derived from the Latin word. Cultivation of vegetable crops. Recently it has been designated as ‘Vegetable Science’. Vegetables are said to be herbaceous plants of which edible plant parts are used for consumption either cooked or salad. It may be stem, root, leaf, flower, etc. It also includes all aspects of vegetable crops e.g. importance, uses, origin and distribution, area and production, soil and climate requirement, botany, nursery raising, cultivation practices, plant protection measures, stage and method of harvesting, post-harvest handling, marketing, export, import, etc. e.g. tomato, brinjal, chilli, cucumber, red pumpkin, watermelon, cabbage, cauliflower, etc.
3. Ornamental Horticulture: It includes various groups of ornamental plants used to decorate in-door and out-door areas. Ornamental horticulture is the study of growing, arranging and tending decorative plants and flowers. It is further divided into.

24.1 Aim

To determine the moisture content in fresh and processed products

24.2 Theory

Estimation of moisture in fresh and processed fruit and vegetable products is important for evaluating their nutritive composition as well as to optimize method for their processing like drying, preparation of different products viz; jams, preserve, candies etc. Moisture is determined by drying the weighed sample to a constant weight in hot air oven and measuring the loss in weight to express the moisture content in percent. This method is used for estimation of moisture in all food products which do not decompose at 100ºC. Moisture content of some commodities (Table 4.1) is as under:

Microbes present in the biofertilizer formulations help in microbial processes in the soil which augment the availability of nutrients to be taken up by plants. They are of different types based on their nature and function such as nitrogen fixing, phosphate solubilizing, phosphate mobilizing or biofertilizers for micronutrients. Plants use different strategies for growth promotion at different stages of plant growth and development. Biofertilizers are mass multiplied cultures of specific beneficial microbial strains which improves the fertility of soil and productivity. The nutrients are added through natural processes of nitrogen fixation, solubilization of phosphorus and synthesis of growth promoting substances to stimulate the plant growth. The main sources of biofertilizers are bacteria, fungi and cyanobacteria.

11.1. Bio-enrichment of chemical fertilizers- A new approach

Yield response of different host plant depends on the mineral fertilizer and its fertilizer use efficiency. Generally, such fertilizers are low in efficiency due to environmental and soil factors. A large portion of synthetic fertilizers are lost due to fixation, leaching, run-off, erosion, volatilization, denitrification and precipitation without reaching plants. Hence, there is an economic loss and water and environmental pollution.

Microbial inoculants applied to the soil enhances the uptake of nutrient by plants and increase the efficiency of synthetic or organic fertilizers. In this context, it is possible to impregnate mineral fertilizers with specific microbial strain. A new approach is to incorporate the microbes or microbial compositions into fertilizer compositions.

Most backaches seem to begin while bending forward to pick up some object from the floor. The pain usually comes on suddenly without warning, and it may be very difficult to straighten up to a standing position again. In most cases it is felt either in the middle of the back or lower down on one side. If a spinal disc is involved, the pain may radiate down the thigh and leg along the sciatic nerve on that side. The most common cause of backache probably arises from failure to get enough exercise to keep the muscles of the back strong and healthy. Flabby muscles then produce a wobbly spine, followed by pain, spasm and misery especially if a disc is ruptured.

Low blood pressure or hypotension is far less common than hypertension or high blood pressure. Sometimes the pressure falls rapidly because of hemorrhage or loss of blood. Low blood pressure may also come on gradually because of slow bleeding in the gastrointestinal tract or through the kidneys or bladder.

Any lack of normal protein in the diet may also lower the pressure because of malnutrition or actual starvation or because of some endocrine disturbance, such as hypothyroidism. In rare cases an islet cell tumor may form in the pancreas producing an excessive amount of insulin and lowering the blood sugar level. Such patients always tend to feel tired and worn out.

Emotional problems are a far more frequent cause of low blood pressure. For instance, some people will fall into a dead faint on hearing bad news. This shock like state is probably brought on by temporary disturbance in the vasomotor system, causing the patient to collapse. To a lesser degree, prolonged disappointment and frustration may also result in a subnormal blood pressure. Relieving the chronic stress of mind may be all that is needed to bring the blood pressure back to normal. One should also add a little more protein to the diet, and be sure to take sufficient rest and exercise.

Homoeopathic treatment for low blood pressure is given below:

26.1. Aim

To determine the pH of food samples.

26.2. Theory

The hydrogen-ion concentration of a food is a controlling factor in many chemicals and microbiological reactions. Pure water has equal concentration of H+ and OH- ions, each having the value of 1×107g/litre at room temperature that is why water is regarded as neutral. The pH of pure water is 7.0, the solution having pH below 7.0 is regarded as acidic while solution with pH above 7.0 is alkaline. Most fruits and fruit products have pH below 4.0 while vegetable; milk and meat products have pH more than 4.0. The pH value of some fruits and fruit products is given in Table 6.1. Thus, with the increase in acidity in the solution, the pH value decrease and vice versa. However, presence of buffer salts helps in maintenance of pH. Estimation of pH of the food is important as the processing conditions for different food products are categorized on the basis of their pH.

ABSTRACT

The Cry2A proteins are promising candidates for managing the development of Cry1Ac resistance in insects. The Cry2A class of proteins is different from that of Cry1A, and experiments have shown that insects resistant to Cry1Ac are not cross-resistant to Cry2A proteins. However, the Cry2A proteins are less toxic than Cry1Ac to the polyphagous pest Helicoverpa armigera. Structure-based protein engineering of Cry toxins may pave the way for directed evolution of variants with broad spectrum of insect species specificity, enhanced potency, and stability. In the present study homology modeling of Cry2Ac was done using DeepView software. The template for Cry2Ac protein sequence (accession number X57252) was selected using ExPdb template search. The raw sequence of the Cry2Ac was superimposed over the Cry2Aa PDB template (1I5P). The superimposed model was submitted to the SWISS Model server. After receiving the Cry2Ac model, energy minimization was done using Gromos96. The threading energy and force field energy were calculated. The homology model of Cry2Ac made by us along with the Cry2Ac model from the SWISS-MODEL Repository was used for comparative analysis of receptor binding regions of Cry2Aa. Results showed variation between the two highly homologous Cry2A proteins in their receptor binding regions which may account for differences in spectrum of insecticidal activity.

There are many manufacturers of biofertilizers in both government and private sectors. Only some of them have been listed here for the information

A neurotic headache, usually reflex in origin and paroxysmal in character.

The causes are many and varying in kind, making both the diagnosis and treatment the most difficult the general practioner encounters,

Migraine is clearly a hereditary condition, and the inheritance is passed down through the females rather than the males. Nervous abnormalities of the female generative organs are the most frequent of the exciting causes. Eye strain; shock; grief; worry; gastro-intestinal irregularities; mental emotions, i.e., anger, fear, love, jealously and remorse may cause migraine at one time and hysteria at another.

Many patients have lived, to suffer from the disease, for fifty years. On the other hand, many are permanently cured. The time needed to complete the cure will depend upon your ability to determine the tissue or organ, which is the cause of the disease, and the faithfulness with which the patient will follow directions.

Next to epilepsy, no disease so frequently presents premonitory symptoms as Hemicrania. These may be blurring of visions, flashes of light, balls of fire, etc.; chilliness; flashes of heat; dizziness; depression or excitation; confusion of ideas; numbness, etc. then comes the pain which is usually one sided, and may be any or all sensations, sharp or dull, continuous or intermittent, etc. There may or may not be tenderness of the affected part.

27.1. Aim

To estimate the titratable acidity of fresh and processed fruit and vegetable products.

27.2. Theory

Most of the fruit, vegetables and their products contain acid or mixture of acids. The acids may occur naturally in the fruit and vegetables or may be added during manufacture of different products or by lactic acid or acetic acid fermentation. Generally citric acid is added in most fruit products while in pickles, sauces and ketchup acetic acid is used. The acids are mainly responsible for the tartness or sour taste, thus estimation of acidity is used as the measure of tartness. They also help in preservation by lowering the pH of the finished products.

Soursop, Annona muricata L., also called as Bullock’s heart, is a larger fruit than custard apple. The flesh is spinny and has a white fibrous flesh in which large black seeds are embedded. It is completely tropical in habitat. It resembles small jackfruit with spines on the surface. Soursop is native to the West Indies. It is very popular in Cuba and Mexico. It is found in India and many other parts of Polynesia.

Uses

Soursop is used for the preparation of sherbet and other refreshing drinks. In Indonesia, the tender fruit is used as a vegetable. Seeds have insecticidal properties and leaves contain an essential oil. Excellent jelly can be prepared from soursop fruit. Unripe fruit powder is prescribed for chronic dysentery. Buds and flowers are considered to be an excellent remedy for cough. Soursop is used as an astringent and also as a blood complement. It has the use as an antidysentric and vermifuge. A black dye is produced from the dye unripe fruit. Seed oil is used as an insecticide and for making agriculture implement. Essential oils extracted from soursop (pulp) have industrial applications and the oils are also thought to improve the flavour of processed fruit products.

It is an acute specific infection of the salivary glands especially of the parotids sometimes submandibular and sublingual glands also; occasionally the latter glands are affected first.

The most affected are the children or young adults of both sexes often living in a school or a barrack and the disease spreads rapidly from one person to another in close contact by droplets. This virus reaches the salivary glands by bloody or lymphatic because mumps is a systemic disease and may cause in addition, inflammation of the meninges, brain, pancreas and the gonads. The disease is more prevalent in winter and spring months. The incubation period is about 3 weeks.

The affected parotid glands and the surrounding tissues become hyperaemic and oedematous, the acinar cells disintegrate with round cell infiltration but later on, heal completely without any scarring.

Testes when involved have much oedema, perivascular infiltration with lymphocytes, localized haemorrhage, destruction of the germinal epithelia and blocking of the tubules with cellular debris. Only certain localized areas are affected, other parts remain unaffected.

An inflammation of the muscular substance of the heart. The condition may be acute or chronic. Acute myocarditis may be sub-divided into three varieties:

1. Acute Parenchyma.
2. Acute Diffuse Interstitial.
3. Acute Circumscribed.

In the first of three classes, there is a glandular degeneration of the muscular fibres of the parenchyma of the heart and an increase in the number of their nuclei. Severe cases of this class often terminate in fatty degeneration.

In the second class, we have round-cell infiltration of connective tissue of the myocardium.

The selection of the site has great importance for the establishment of a fruit orchard successfully. Establishment of orchard is a long term activity with huge investment. Hence, any mistake committed during site selection, it is difficult to rectify it later. Successful orchard is not only related with yield but also its economic production and efficient disposal of produce. For economic orchard establishment; some of the points which need to be considered, while selection of site. These are as follows.

1. Climate and soil: These are two important natural factors, which decide the success or failure of orchards. Before selection of site, climate and soil requirement for selected fruit crops needs to be studied thoroughly and accordingly site must be selected. On the other hand if a site has already been selected; then fruit crop selection needs to be done as per the existing climate and soil of that selected site.
2. Topography: Slope of site more than 15% makes the land almost unsuitable for cultivation. Land with 4% slope may be kept clean without much loss. Uneven land involves heavy expenditure in leveling. Southern slopes are generally warmer and affect the plant growth in a manner different from the northern slopes. It is due to the difference in quantum of receiving sunlight. Hence, the site with southern slopes may be selected for sun loving plants.

29.1. Aim

To estimate ascorbic acid in fresh and processed fruit and vegetable products.

29.2. Theory and Principle

Fruit, vegetables and their products are important sources of ascorbic acid. The ascorbic acid is present in sufficient quantity in aonla, guava, grapefruit, lemon, pineapple, strawberry fruits etc. Barbados cherry is the chief source of vitamin C. The products manufactured from these fruits are also considered as rich in ascorbic acid and the contents available in the commodities can be detected by using 2, 6 dichloro-phenol - indophenol visual titration method.