eChapters

Partial or complete loss of hair on the scalp is rather common among them. This is more or less to be expected in the female. Premature baldness is a tendency in certain families. Thinning of the hair is often seen in older women, but complete baldness in the female is fortunately rare. Temporary baldness may occur following a fever or some prolonged illness. The hair usually returns after the patient has recovered from the illness.

Sudden loss of the hair in certain areas, a condition called alopecia areata, may sometimes follows extreme nervous shock. This may clear up in a few weeks, although sometimes it will recur. In females excessive hair loss is seen after delivery and during lactation. This is only due to hormonal changes in the body and due to suckling of the milk by the child. This disappears after the child stops weaning. It is not a permanent problem. In children falling of hair occurs due to many reasons such as severe headache, dim eyesight, after a prolonged illness, use of different shampoos and soaps, after a severe head injury and a familial tendency. Generally it is observed that if the parents have thin hair and early loss of hair in their life so is their children.

Many people use various shampoos and creams to prevent hair loss but with no gain. Homoeopathy has a very good treatment for hair loss. Some of the important medicines indicated in the hair loss are given below:

Asthma is a condition of sudden paroxysmal, expiratory, wheezing dyspnoea caused by an abnormal response of the respiratory center to stimulus causing hyperactivity of the vagal endings in the broncho-constricting muscles resulting in bronchial spasm; with it, hyperaemia oftlte mucous membrane followed by excessive bronchial secretion is associated.

This is true spasmodic asthma and in this other conditions of periodic expiratory dyspnoea with a different primary factor should not be included.

The disease has in most a family history and is more common in males; sometimes found in young children but more frequently begins in early adult life. Some of these have definite bronchitis also but in the most, the main is paroxysmal bronchospasm no physical sign obtained in the lungs.

The factors concerned in asthma are (i) bronchospasm from causes central and peripheral, (ii) oedema of the bronchial mucous membrane from allergy. Neuropathic predisposition.

The more immediate causes are (i) allergy, (ii) peripheral stimuli from their site of production, grouped as (a) naso-pharyngeal, (b) alimentary, (c) endocrine (d) broncho-pulmonary (iii) constitutional disturbances.

For ages, man has consumed alcohol to forget his sorrows, to lead him into the realm of happiness. John Keats (1795 -1821), the great romantic English poet, listened to the full-throated music of a nightingale and wanted to become one with that moment of happiness. He thought of taking 'a beaker full of the warm south, of vintage, that has been cooled a long age in the deep delved earth⁷, drinking which he could ' leave the world unseen', and fade away into the dim forest with the singing bird. But Keats was romanticizing the effect of wine on the human mind. A moment later, he decided to give up the idea of taking the assistance of Bacchus and his leopards and switched over to reaching the bird and its music on 'the viewless wings of poesy⁷. This was definitely a wise decision because the alleged euphoric effect on alcohol is a myth. In fact alcohol dulls the brain and retards its activity.

Alcohol is basically a drug. Man consumes it more than any other drug. No form of alcohol, however mild, whether wine or beer or cider, can do good to the human system and health.

It is fallacy to believe that alcohol stimulates the brain. In reality, alcohol depressed the brain. It slows down various centers of control. When alcohol is swallowed, it goes into the stomach where a fraction of the dose enters the blood stream through the blood vessels of the stomach walls. From the stomach, alcohol has to pass into the small intestine. The carbon dioxide in soda water accelerates this flow of alcohol from the stomach into the intestines. This is what makes whisky and soda more dangerous than whisky and water.

Pimples are very common in young people during their teen years. This condition, which is also known as acne, is apparently due to certain hormone changes in the body. It is particularly prevalent during early adolescence, from 12 to 16 years of age, and is more likely to occur in those who have an oily skin. In the early stages a blackhead or comedone blocks the outlet of an oil gland within the skin. This is not a serious problem, for it leaves no permanent effect.

If the sebaceous duct ruptures and the oily material ooze out between the layers of the skin instead of coming to the surface, there may be a cyst or swelling known as a sebaceous cyst. These are somewhat disfiguring, but not harmless unless they become infected and form small abscesses. When these heal they may leave ugly scars. Severe acne may occur on the shoulders, the back and chest, as well as the face and neck.

Effects of diet: Acne is not caused by diet, but certain foods definitely aggravate this condition, especially in some patients. Rich foods, such as chocolate, nuts, cola drinks, ice-cream, fatty meats, and excessive amount of sugar, all seem to aggravate this condition. Certain drugs, such as those containing bromides and iodides, may also play a part in some people.

Some girls develop one to two pimples just before menstruation. These only last a day or two and are of no consequence. But when acne continues over months or years, it is very distressing, especially to a young woman, and something should definitely be done to clear up the condition. Nervous tension due to family trouble may also play a part in continuing this difficult problem. Even in its mildest form, acne usually persists for a year or more until the hormone system of the body has become fully adjusted.

This chapter provides a readily available means of reference for helping in life threatening emergencies, accidents and for providing first aid, where any delay caused by the lack of medication or non-availability of the doctor can have serious consequences either in terms of life or in terms of resulting serious sickness.

As soon as possible competent medical help should be availed of in emergencies and accidents. In the meanwhile the homoeopathic medicines suggested hereafter would provide relief, would halt deterioration in most cases and may save life in many instances.

Emergencies can be life threatening. One of these can be severe haemorrhage, where copious bleeding can lead to death. Obstruction of breathing for different reasons is a matter for concern. Serious accidents are life threatening because there may be unmanageable bleeding, shock and pain.

Below, a list of few medicines and other items required for first aid in emergencies has been given. This can be helpful in homes, in factories and offices.

Homoeopathy system of medicine is the system of healing suited to all the young, and the old, the rich and the poor, the people in the developed as well as the underdeveloped countries and for persons in any place or climate. This is not to take away from the value and utility of the other prevalent systems of medicine. The aim of every science of healing is the relief of sick. Every system is successful in its own fashion. Homoeopathy too fights sickness and does so with remarkable results. Additionally the cost of diagnosis in this system is negligible because the diagnosis is by and large symptomatic. The medicine itself is inexpensive. Notable efficacy and low costs are the strong points of homoeopathic medicine. There are no toxic effects of the drugs, no side effects. The time taken for the medicine to show improvement is not long. Homoeopathic medicines are easy to store and have a long life.

Homoeopathy has been attacked again and again on the ground that the potencised drugs cannot be tested in a laboratory. But that way a number of phenomenon is still outside the pale of conventional science. However, laboratory tests have been going in many countries and certain phenomenon not acceptable to conventional scientific beliefs have been observed.

The homoeopathic system of medicine develops for its special use, the inner medicinal powers of the crude substances by means of a process peculiar to it and which has never been tried, whereby they only all become immeasurably and penetratingly efficacious and remedial. Even those that in the crude state give no evidence of the slightest medicinal power on the human body.

This remarkable change in the qualities of natural bodies develops the latent, hidden powers, which influence the body, change the well-being. This is affected by mechanical action upon their smallest particles by means of rubbing and shaking and through the addition of an indifferent substance, dry or fluid. This process is called dynamization; potencizing and the products are dynamizations or potencies in different degrees.

The homoeopathic materia medica consists of around 1000 drugs, the characteristics of which have been verified or proved. Homoeopathy is unique in that it uses no drug, which has not been proved. The proving has been done by administering pure medicines to healthy human beings and recording in minute detail the effects, mostly adverse, produced by such administration. The tests were conducted on men and women who were mostly medical people and students who were not sick. The drugs were administered in doses consisting of crude drugs or homoeopathic potencies. These volunteers were then asked to describe the effects of the drugs in their own words. These descriptions were noted down by the doctors conducting the experiments and were carefully recorded. Hundreds of thousands of such symptoms were recorded while proving the homoeopathic drugs and the materia medica forms a record of the best proving along with other data collected as a result of laboratory tests and experiments.

In paragraph 25 of the 'Organon of medicine', Dr.Samuel Hahnemann the founder of the system laid down the basis of homoeopathy in the following words: "Now, however, in all careful trials, pure experience, the sole and infallible oracle qi healing art, teaches us that medicine which, in its action on the healthy human body, has demonstrated its power of producing the greatest number of symptoms similar to those observable in the case of disease under treatment, does also, in doses of suitable potency and attenuation, rapidly, radically and permanently remove the totality of the symptoms of this morbid state, that is to say the whole disease present, and change it into health; and that all medicines cure, without exception, those diseases whose symptoms most nearly resemble their own, and leave none of them uncured."

Homoeopathic treatment is based on an overall understanding of the symptoms in the body and mind created by a sickness. Dr. Samuel Hahnemann, the founder of the homoeopathic science of healing, after years of experiment and trial, came to finding that symptom - similarity is the curative method of medically curable diseases. In paragraph 25 of the 'Organon of Medicine⁷, he formulated the principle of 'like cures like' in these words: " Now, however, in all careful trials, pure experience, the sole and infallible oracle of the healing art, teaches us that actually that medicine which, in its action on the healthy human body, has demonstrated its power of producing the greatest number of symptoms similar to those observable in the case of disease under treatment, does also, rapidly, radically and permanently remove the totality of the symptoms of this morbid state, that is to say, the whole disease present, and change it into health; and that all medicines cure, without exception, those diseases whose symptoms most really resemble their own, and leave none of them uncured."

The aforesaid indicates the importance of symptoms in the diagnosis and treatment in homoeopathy. If a drug creates a symptom during the course of proving, then it can be cured by a potencised dose of the drug; whatever may be the name of the disease. For instance, in proving GLONOINE produces a bursting, throbbing sensation in the head in a healthy person. Conversely any surging of the blood to the head requires GLONOINE. This can happen in a sun - stroke, in high blood pressure, in cerebro - spinal meningitis. KALI BICROMICUM produces in proving, a tough, stringy discharge of mucus. In homoeopathy, KALI BICROMICUM cures a similar discharge whether from the nose inwards from mouth, from fauces, from pharynx, from larynx, trachea, bronchi, vagina, and uterus or from any mucus membrane. CACTUSGRANDIFLORUS produces in pure form and gross doses, constriction of the heat and a constriction, anywhere in the body, which is like an iron band being tightened. The same sensation of constriction leads us to the use of CACTUS in a homoeopathic dose and it need not be confined to the heart. There can be constriction of the scalp, in the oesophagus, in the chest, in the abdomen, bladder, uterus, vagina or rectum.

This individualizing examination of a case of disease, for which Dr. Hahnemann give general directions, of which the practitioner will bear in mind only what is applicable for each individual case, demands of the physician nothing but freedom from prejudice and sound senses, attention in observing and fidelity in tracing the picture of the disease.

The patient details the history of his sufferings; those about him tell what they heard him complain of, how he has behaved and what they have noticed in him; the physician sees, hears, and remarks by his other senses what there is of an altered or unusual character about him. He writes down accurately all that the patient and his friends have told him in the very expressions used by them. Keeping silence himself, he allows them to say all they have to say, and refrains from interrupting them (every interruption breaks the train of thought of the narrators, and all they would have said at first does not again occur to them in precisely the same manner after that.) unless they wander off to other matters. The physician advises them at the beginning of the examination to speak slowly, in order that he may take down in writing the important parts of what the speaker says.

He begins a fresh line with every new circumstance mentioned by the patient or his friends, so that the symptoms shall be all ranged separately one below the other. He can thus add to any one that may at first have been related in too vague a manner but subsequently more explicitly explained.

Dr. Samuel Hahnemann laid down the fundamentals of the then new doctrine of homoeopathy in organon of medicine. As observed by him in the 'Introduction⁷ to this work, till then no one had ever taught this homoeopathic mode of cure, no one had carried it out in practice.

Dr. Hahnemann was of the view that the art of the medical practice "should. Rapidly and mildly, effect an unalloyed and permanent cure, and restore to health by means of the smallest doses of a few simple medicines carefully selected according to their proved effect, by the only therapeutic law comfortable to nature, that is Similia Similibus Curentur".

Homoeopathy is based on the following principles:

"The physicians high and only mission is to restore the sick to health, to cure, as it is termed"

The highest ideal of cure is rapid, gentle and permanent restoration of health or removal and annihilation of the disease in its whole extent in the shortest, most reliable and most harmless way on easily comprehensible principles.

Unprejudiced observer of a sick person takes note of nothing in every individuars disease except the changes in the health of the body and of the mind, which can be perceived externally by means of the senses. "All these perceptible signs represent the disease in its whole extent, that is, together they form the true and only conceivable portrait of the disease"

The homoeopathic system of medicine is one of the finest systems of cure available in the world. It achieves in totality the ideal laid down by its founder, Dr. Samuel Hahnemann:

"The highest ideal of cure is rapid, gentle and permanent restoration of the health, or removal and annihilation oftlie disease in its whole extent, in the shortest, most reliable, and most harmless way, on easily compreltensible principles".

In homoeopathy, not a single drug is used without knowing its effect on healthy human beings. Hence there is no shooting in the dark. The system operates, by and large, without laboratory tests and other expensive means of diagnosis, which are being used when not needed and that too, unfortunately on patients who can hardly afford the expenses.

Cost wise, there is no medical system in tlie world, which is as inexpensive as homoeopathy. No patient who requires a speedy cure without side effects can ignore homoeopathy; no doctor who has nothing but the good of his patient at heart can overlook the utility and effectiveness of homoeopathy and no Government which has to balance its budget can afford not to use homoeopathy in hospitals and other institutions run for the health and care of the sick and thus it is the most cost effective treatment

The discovery of homoeopathic system of medicine makes a fascinating story.

8.1 Black Pepper Breeding

Black pepper (Piper nigrum L., family:Piperaceae), known as the ‘King of Spices’ and “Black gold”, is one of the important spices since time immemorial and perhaps the oldest spice regarded globally. It is a perennial climber grown for its fruits that are used as spice with plethora of medicinal properties. Black pepper is also very important in traditional medicine (Ravindran, 2000). However, it is mainly valued for its characteristic pungency and flavour, as an ingredient in food preparations and also as a condiment.

Genetic Resources

World’s largest collection of black pepper germplasm is maintained by the Indian Institute of Spices Research (IISR) at Kozhikode, Kerala, containing local cultivars, wild forms collected from the area of origin and related species. At present there are about 3466 accessions, consisting of 1266 wild relatives, 2062 cultivars, in addition to 9 exotic collections maintained in India. These collections were characterized and evaluated to estimate the genetic diversity for various yield and quality characters. Cultivar diversity is one of the principal components of diversity in black pepper. The cultivars are evolved directly from the wild P. nigrum.

7.1 Plant Tissue Culture

Spices have great socio-economic impact in moden food habit of human but loss of resources day by day due to various reasons (industrialization, high population pressure) make the access of these valuable spices limited to people. Low production is general phenomena of common spices due to lack of high yielding varieties. Hence there is a need to improve the propagation method to meet the gap between demand and supply. Conventional vegatative propagation method is common approach for propagation but low multiplication rate, seasonal dependency and susceptible to abiotic stresses and diseases are major problems to achieve the optimum productivity. To overcome these drawbacks scientists have chosen the plant tissue culture technique (a modern approach of biotechnology which deals with the propagation the valuable/endangered plants under laboratory condition). Details about this technique and its role on spices are given below.

Plant Tissue Culture: Basic Components and Objectives

The technique of growing of a whole plant cell, tissue or organ under an aseptic condition in suitable synthetic medium is termed as plant tissue culture (Thorpe, 2007). De-differentiation and re-differentiation are two essential fundamental processes which help in the physio-morphological development of the plant.

6.1 Introduction

Plant breeding is the indispensible component of elevating production and productivity of spices by evolving high yielding and/or with good quality type. Thereby, the practice of identification and selection of desirable traits and introgression of those characters in a single plant is being done since the beginning of organized agricultural activities by humankind. The act of plant breeding was reshaped and got a momentum with a scientific basis after the discovery and rather rediscovery of Mendel’s laws. Worldwide, humankind could enjoy the direct benefits of methodical plant breeding during green revolution. Manipulation of genetic architecture for creating variation in the population and successive selection is the basic of all kinds of crop breeding. But the process of creation of variability and selection differs from crop to crop depending on their mode of reproduction. It is comparatively easier to get a new homogeneous population in self pollinating plants. Therefore, segregating generations are handled differently in these two categories of self and cross pollinated crops.

Pollination

Pollination is the act of transfer of pollen from the male reproductive organ of a plant to the female reproductive organ of same or another plant.

Pollination is of two types

5.1 Self Pollination

It is the transfer of pollen from the anther to the stigma of the same flower from where the anthers have been generated. Thus, self pollination happens to be denominated as autogamy where auto means “same” and gamy means “union”. Obviously, self pollination is found in bisexual flowers. But self pollination also occurs when pollen is transferred from one bisexual flower to the stigma of another bisexual flower of the same plant. This kind of autogamy is known as geitonogamy. So, self pollination has to be defined keeping the parent plant on the main focus not the flower. If genetical consequences are considered, self pollination promotes the occurance of purelines or homozygotes. Self pollinating species developed themselves evolutionary fit due to homozygous balance and do not exhibit significant inbreeding depression.

Cultivated plants in general including the spice crops observed today have been evolved through selections from their weedy and wild types. Apart from this, there had been another force which could bring the wild forms into cultivated forms named human intervention which is actually the process of domestication. Natural mutations and genetic recombination after selfing and crossing also played a significant role in the entire process. Whereas, natural forces take a long time to come into act, the techniques of plant breeding was devised and fine tuned throughout passing of times, essentially to accelerate the speed of evolution using these natural phenomenons. It was, therefore, mandatory for us to know the modes of reproduction and pollination to create variability in natural population and make all thise happen to what we see today in organised cultivated forms. Moreover, knowledge on reproduction of different plant species helps the breeder to choose the right breeding procedure to be used for crop improvement. Modes of reproduction help to evaluate the genetic constitution of crop plants by determining the extent of homozygosity or heterozygosity which, in turn, are unanimously applicable to any plant. To comprehend modes of reproduction in spice crops effectively, understanding about the floral biology is absolutely necessary. Plant reproduction, a process by which one species gives birth to its offsprings, may be of two types

Since historical times, Indian spices have been contributed not only to the country’s wealth and prosperity but also to the subcontinent itself as a whole. Spices precisely witnessed many wars, bloodshed, armoury alongwith the glowing accounts and adventurous voyage by Pliny, Ptolemy, Marco Polo, Vasco da Gamma and other travelers. The Southern parts of India, especially the Malabar Coast, with its abundance of the World’s best pepper, cinnamon and ginger was enormously creating impact of a plethora of spices which attracted the foreign trade and continue even today by creating a unique brand value globally. In the past, invaders travelled to India by overcoming many odds and barriers with the prime focus on capturing spice trade and dominating the trade scenario of those days throughout the world. As a result, gradually Indian spices travelled far and wide to traditional destinations by providing flavor, aroma, pungency, taste and colour to a variety of foods and beverages.

It was the end of 18^th century, from when scientific research began on the idea of origin of cultivated plants. The pioneers of this field were Darwin, Alphonse de Candolle, N.I Vavilov and many more. Charles Darwin wrote his famous book ‘On the Origin of Species by Means of Natural Selection’, or the ‘Preservation of Favoured Races in the Struggle for Life’ published on 24^th November 1859. According to him, cultivated plants may have originated from its wild form after a series of intense modifications. Afterwards, a Swiss botanist Alphonse de Candolle studied 247 cultivated plant species and documented his work in the book “Origin of cultivated plants” in 1886. Later, Vavilov first identified the Centres of origin. He could determine the origin of plants by analyzing the pattern of variation and proposed that, crop plants have been evolved from their wild species in the geographic region of the greatest genetic diversity of that particular plant. That area is known as the primary centres of origin of the particular plant. But, some crop species were found to show substantial genetic diversity even though they did not originate there. Such areas are known as secondary centres of origin for that particular species.

A. General Morphology

2.1 The Root

It is the organ of a plant that typically lies below the surface of the soil.The root is best defined as the non-leaf, non-nodes bearing parts of the plant’s body. However, important internal structural differences between stems and roots exist. Root spices are, rather surprisingly, rare. Some popular examples of spices having roots are: Apium graveolens (Celery), Armoracia rusticana (Horse radish), Coriandrum sativum (Coriander), Ferula asafoetida (Asafetida), Glycyrrhiza glabra (Licorice), Levisticum officinale (Lovage), (Petroselinum crispum (Parsley), etc. All of the mentioned plants have aromatic roots.Roots of gingiberaceae are adventitious and storage roots arise from among the nodes of these scales.

Plant breeding is described as an art and science of changing the genetic architecture of plants with a goal towards betterment of mankind. Apart from this generalized concept, plant breeding is accomplished through inter-disciplinary mode assorted from simple selection in a variable plant-population having some desirable traits to the application of biotechnology and more. Worldwide, cultivars of different crops are routinely manufactured by Multinational and National level Industries, Government Institutions, Universities, Crop specific Organizations, professional plant breeders and even by the individuals such as gardeners and farmers.

Plant breeding is basically operated by the principles of Genetics where selections are practiced from a variable population and the selected variation needs to be inherited. Three categorized broad goals of plant breeding are 1) high yield 2) uplift in quality and 3) resistance against biotic and abiotic stress.

Spice crops are known to add nutritive value and increase the palatability as well as quality of food. Therapeutic uses of spices are also well recognised. The spice industry in India and trade has shown stunning progress over the last 5 years with 120% increase in revenue which is very close to touch $ 3 billion. East Asia is the major market for spices, followed by America and the European Union and the world spice trade has almost touched $ 17 billion by 2020. So, increased production is of paramount importance across the world and the potential is increasing. On the other hand, quality improvement is one of the primary objectives of spice crop breeding apart from achieving high yield. Cultivar and species diversity are the principal components of diversity of spices (Sasikumar et al. 1999; Krishnamoorthy et al., 1997; Prasath and Venugopal, 2004). A good amount of variability is found in many important Indian spices like black pepper, cardamom, cinnamon, curry leaf, ginger, garcinia, tamarind and turmeric. A number of cultivars have been released followed by intra-germplasm evaluation and selection in spices. Contribution of recombination breeding is less in spice crops with regard to other field crops and a number of valuable varieties have been bred by clonal and seedling selection (Peter, 2008). Deployment of high yielding, water use efficient, climate resilient genotypes and technologies are also necessary.

The biofertilizer technology has paved way for the manufacturers and entrepreuners to establish a biofertilizer production unit due to an increasing demand and awareness among farmers. Biofertilizer production units have already been started with most of them in the southern states of India. The biofertilizers can be produced using different kinds of carrier materials (peat, coal, lignite, clays, inorganic soil), organic materials (composts, soybean meal, wheat bran, sawdust, etc.), or inert materials (e.g., vermiculite, perlite, kaolin, bentonite, silicates). Natural carriers like peat, plant waste materials, composts, sawdust, sludge lignite, clays, coal, vermiculite, perlite and bentonite and mixture of organic and inorganic materials may also be used.

The Government of India is also encouraging a low cost technology by providing a subsidy to establish a biofertilizer production unit . The major equipment’s and other materials required for a biofertilizer production unit with the capacity of 150 metric tonnes/annum are given below

There are many manufacturers of biofertilizers in both government and private sectors. Only some of them have been listed here for the information

The soil fertility is depleting in the current scenario of gap between nutrient removal from soil and supplies. Moreover, increase in the fertilizer prices has become unaffordable to small and marginal farmers. There is also a growing concern about the environmental hazards and its threat to sustainable crop production. Indiscriminate use of synthetic fertilizers for intensive crop production has resulted in large quantity of nutrient (particularly P) accumulation in soils which has resulted in generating a dead soil. In this context, the long term use of biofertilizers are considered to be economical, eco-friendly, more efficient, productive and accessible to marginal and small farmers over chemical fertilizers (Subba Rao, 2001). Biofertilizers mainly consists of specific living cells which supplies nutrients to plants through their root system. Microbes in these fertilizers provide nutrients to the plants by nitrogen fixation, phosphate solubilization, phosphate mobilization, and promotion of beneficial rhizobacteria (Bhat et al., 2010). Biofertilizer application improves the supply of nutrients, organic carbon, accumulation of soil enzymes, productivity, soil fertility index, economy of farmers, and maintenance of sustainability in natural soil ecosystem and horticultural crops. The production of efficient and sustainable biofertilizers for crop plants needs to be undertaken to make the biofertilizers popular and efficient. The important and specific research in future may be focused on the following aspects.

Microbes present in the biofertilizer formulations help in microbial processes in the soil which augment the availability of nutrients to be taken up by plants. They are of different types based on their nature and function such as nitrogen fixing, phosphate solubilizing, phosphate mobilizing or biofertilizers for micronutrients. Plants use different strategies for growth promotion at different stages of plant growth and development. Biofertilizers are mass multiplied cultures of specific beneficial microbial strains which improves the fertility of soil and productivity. The nutrients are added through natural processes of nitrogen fixation, solubilization of phosphorus and synthesis of growth promoting substances to stimulate the plant growth. The main sources of biofertilizers are bacteria, fungi and cyanobacteria.

11.1. Bio-enrichment of chemical fertilizers- A new approach

Yield response of different host plant depends on the mineral fertilizer and its fertilizer use efficiency. Generally, such fertilizers are low in efficiency due to environmental and soil factors. A large portion of synthetic fertilizers are lost due to fixation, leaching, run-off, erosion, volatilization, denitrification and precipitation without reaching plants. Hence, there is an economic loss and water and environmental pollution.

Microbial inoculants applied to the soil enhances the uptake of nutrient by plants and increase the efficiency of synthetic or organic fertilizers. In this context, it is possible to impregnate mineral fertilizers with specific microbial strain. A new approach is to incorporate the microbes or microbial compositions into fertilizer compositions.

It has been well established that the biofertilizers are beneficial to plants and are cost-effective inputs for sustainable production system. However, there are some constraints with respect to its production, which are presented below:

10.1 Production constraints

10.1.1 Raw material

Eventhough there are large number of biofertilizer formulations, only few are considered as good carrier materials for biofertilizers such as peat and lignite. Moreover, its availability in India is limited and the quality is lacking for these carrier materials. Hence, a cost effective and locally available material with increased shelf-life is the need of the hour.

10.1.2 Specificity and availability of strains

As the microbial strains used as biofertilizers are specific to soil and agro-ecological conditions, availability of specific strains for the specific host and soil edaphic factors are inadequate.

Quality Control is very important in biofertilizer production, which determines the efficiency of microbial strains. Generally, the quality control based on BIS are voluntary in nature. However, some of the biofertilizers are brought under the ambit of Fertilizer Control Order 1985 (FCO) during 2006 (Yadav and Chandra, 2014). At present, biofertilizers are under the FCO with specific quality standards. Biofertilizer production and its sales can be regulated and is a mandatory requirement for registration of manufacturing unit with the State Fertilizer Controller (who is generally the Commissioner or Director of Agriculture Department) under the statutory provision of FCO. Moreover, some officers of the Agriculture Department have been declared as Fertilizer Inspectors at the district level, who are authorized to inspect production and storage facilities and draw samples for quality analysis. National Centre of Organic Farming (NCOF), Ghaziabad under the Govt. of India and its six Regional Centre’s located at Bhubaneswar, Bangalore, Jabalpur, Nagpur, Hissar and Imphal have been declared as testing laboratories. Under the provisions of the FCO act, State Governments can also develop their own quality control laboratories and notify them under the FCO (1985). Peat, lignite, peat soil, humus, wood charcoal or similar material favouring the growth of microbial strain could also be used. The FCO specifications for different biofertilizers are presented below:

Biofertilizer is already proven to be beneficial for agricultural and horticultural crops. The most important pre-requisite for the success of biofertilizer application is the presence of high population of viable cells which will show the desired effect on plants. Biotic interactions along with competition from native microflora is a major challenge to an applied strains of biofertilizers. The formulation is an important parameter for biofertilizer production containing efficient microbial strain with suitable carrier material and additives. The microbial strain needs suitable protective environment to increase their efficiency at the target site and also make it user-friendly. Performance of biofertilizers under field condition is the major requirement for inoculants. Inadequate formulations and poor inoculant quality are the major hurdles for the successful use of biofertilizers.

Formulation of biofertilizers has to be consistant during production, distribution, storage and transport to the farmer/end users. The shelf-life of inoculated microbial fertilizer depends on the formulation with suitable carrier material. If local production and distribution of an efficient native biofertilizer formulations are developed, the present problems in the field performance of biofertilizers could be significantly solved. Specific formulations of native strains for use in saline soils, acid soils, drought, high temperature and eroded soils requires specific formulations. Therefore,use of native strains of biofertilizer formulations which are tolerant to the abiotic stress is an important approach. The formulations of biofertilizers are challenging and limited success has been observed in the commercialization of new biofertilizers. Hence, the search for new formulations has been a continuous approach. However, the choice of the formulation depends on the application method, available equipment, farmer’s convenience, plant inherent characteristics and developmental stage, cost, mode of action and colonization pathway of the inoculated strain. Conventionally, solid based formulations are used, which have problems of low viability during storage and application.

Biofertilizers can directly or indirectly influence the growth of plant. The direct influence can be through production of phytohormones or making the nutrients available to plants. The indirect effect can be through management of plant pathogens through biological control. Therefore, the purposeful introduction of biofertilizer inoculants to plant microbiome represents an environmentally sound option that holds a prominent position in order to reduce the dependency on agrochemicals (Adesemoye and Kloepper, 2009; Abhilash et al., 2016; Aloo, et al., 2019).

Microbes in soil can rarely exists as single cells and survive near the plant root canopy or in bulk soils or endophytes or even on plant surfaces (Lorito, 2006). The soil microflora occurs as complex members of microbiota (Hacquard, 2015). Microbial consortia (MC) are unique mixture of organisms which respond to the environment stress and adapt to stresses due to internal beneficial interactions among the microbial community. Ecosystem’s physiological functions by microflora is carried out by more than one microbial species with the functional diversity and succession among different microbial components. The microbial balance among the different microflora of a MC will consist of a continuous shift between actively growing cells and non-dividing cells of the microbial consortia. It has been reported that different microbial populations “talk” to each other by “quorum sensing” mechanism in a given environment by exchanging specific chemical signals (Thompson, 2015). Natural microbial consortia holds many specific properties like stability, functional diversity and perform complex functions. The natural consortia have generated an interest in manpulating synthetic consortia for the benefit of application (Minty, et al., 2013).

Fruits, vegetables, ornamentals, plantation crops and spices, medicinal and aromatic plants, tubers, are the major horticultural crops contributing to the economic growth in India. Even though the horticultural sector is growing fast, with India being the second largest producer of fruits and vegetables in the world, the productivity and quality have to be improved in order to meet the demand of increasing population. Under these circumstances, ecofriendly techniques needs to be adopted which could ensure food security and sustainable production. Indiscriminate application of agrochemicals and chemical fertilizers in horticultural crops and their residues could harm the human health, soil and environment. Horticultural crops need an ecofriendly technique, as most of the horticultural products are directly consumed. Hence, there should not be any residues of agrochemicals in food products. In the current context, use of ecofriendly, cost effective and organic-based inputs like biofertilizers can maintain health of the soil and good quality in horticultural crops. The application of biofertilizers offers an attractive and sustainable means of improving the quality of food products. Biofertilizers like Azotobacter, Azospirillum and AMF and PGPRs not only supplies the required nutrients to plants, but improves the fruit quality and quantity (Pathak et al., 2017).

The inoculum dosage and method of application determines the efficacy of biofertilizers. The quantity of biofertilizers to be applied depends on the type of crop, seed rate and soil edaphic factors. There are 3 methods for inoculation of biofertilizers

Seed treatment: One packet containing inoculant (200 g) is mixed with 200 ml of rice gruel or 40 % gum arabic solution to make a slurry (Fig. 23). Then the seeds required for an acre are mixed in the slurry in such a manner that they have a uniform coating of the inoculant over the seeds. Then, the inoculated seeds are shade dried for 30 minutes. The shade dried seeds should be sown within 24 hours. One packet of the inoculant (200 g) is normally sufficient to treat 10 kg of seeds.

Benefit of biofertilizers have been extensively studied for various agricultural and horticultural crops. However, the effectiveness of inoculated biofertilizers are influenced by several factors. If the use of biofertilizers have to be popularized in horticultural crops, the major factors affecting their performance particularly in relation to soil, plant, quality of the product and application methods have to be addressed. Factors affecting the performance of biofertilizers are:

4.1 Quality of the product

4.1.1 Production process

The high quality of biofertilizers are determined by the production process (Bashan et al., 2014). At the same time, the population density of mother culture and the quality of the final products are directly related (Stephens and Raskm, 2000). As the inoculum consists of a strain, the relationship among the microorganisms interaction in the rhizosphere have to be understood. In the case of more than one microorganisms, the efficiency depends on the various mode of action by various microorganisms which might overlap with antagonistic acitivities (Vassilev et al., 2001, 2006 b). The relationship of microorganisms with plants is not based on the taxonomic diversity, but their functional diversity (Maherali and Klironomos, 2007).

The efficiency of biofertilizers could be assured by enhanced shelf life of the inoculant and biological traits which are a challenge for developing a formulation (Bashan, et al., 2014). Hence, either single or consortia of microorganisms formulation with or without additives determines the efficacy of the biofertilizers. The formulation determines shelf-life during storage and transport, which enhances the persistence of the strain in soil and get maximum benefits to the host plants after inoculation (Manikandan et al., 2010; Schoebitz et al., 2012). Different kinds of carriers are used in the formulation process which affects the overall quality and efficacy of the biofertilizers (Bashan et al., 2014; Herrmann and Lesueur, 2013). Granular inoculants showed good results under stressed soil conditions (Clayton et al., 2004; Lupwayi et al., 2006). Encapsulation of the microbial strain into polymers allow diverse compositions and structures (Vassilev et al., 2005) but, have limitations for commercial production (Bashan, et al., 2014; John et al., 2011). Use of additives might improve the shelf life of the product (Bashan et al., 2014; Malusà et al., 2012; Herrmann and Lesueur, 2013) with introduction of specific substances to enhance the efficacy of biofertilizers.

Biofertilizers improve the plant growth due to changes in the microbial community of rhizosphere by production of several substances. Normally, biofertilizers promote plant growth either by facilitating nutrient absorption or by secretion of plant hormones. Biofertilizers also cause inhibitory effects by acting as biocontrol agents on various pathogens so that the growth of plant is enhanced. The mode of action could be either direct or indirect methods

2.1 Nitrogenous biofertilizers

These biofertilizers augment nitrogen to the plants through fixation of atmospheric nitrogen, either symbiotically or asymbiotically. The major nitrogenous biofertilizers are:

2.1.1 Rhizobium

The word Rhizobium is a Greek word where “rhiza” refers to root, and “bios” refers to life. Rhizobium was first named by Frank (1889) which means ‘root living’ in Latin (Fig. 3). Some species of Rhizobium were later moved to new genera based on phylogenetic analysis. It consists of 49 rhizobial species and 11 non-rhizobial species.

In modern agriculture, use of synthetic chemical fertilizers are essential in agriculture for sustainable yield but, these have resulted in environmental pollution. The use of synthetic fertilizer could result in serious human health issues and also affects the soil microflora, besides being costly due to high production cost. Further, excess use affects soil processes and result in nutrient imbalances in plants with increased susceptibility to pests and diseases and also affects the symbiotic associations. Under these circumstances, biofertilizers could be an alternative for integrated nutrient management system in horticulture. The entire crop production system depends on soil beneficial microflora and their activities, which makes them highly potential alternatives to synthetic chemical fertilizers.

Biofertilizers or microbial fertilizers are essential for eco-friendly and sustainable cultural practices. The term “biofertiliser” has been defined in several ways due to its unique relationships between the plant and rhizosphere microflora. Biofertilizers mainly include nitrogen fixing bacteria, phosphate solublizing and potash solubiling bacteria.

India is bestowed with varied climatic conditions and a variety of horticultural crops are produced. It is estimated that 20-30% of the produce is lost before consumption because of various reasons including improper storage and this is where the need of processing for preservation arises. There are various ways of preserving the produce and various ways to process it.

Preservation is defined as methods of extending the shelf life of the produce in its original form or in a different usable form. The basic principle of preservation involves strategies which aim at reducing the spoilage. The horticulture produce is spoiled by microbial infection or infestation. Prevention of microbial damage can be achieved by keeping the produce away from microbes, by removing the microbes, obstructing the proliferation and by killing the microbes. Infestation in the harvested fruits may lead to other microbial infection and spoilage due to rotting.

Horticultural crops are highly perishable and the post-harvest loss is estimated to be 20-30% every year. There are various factors associated with post-harvest losses. It includes pre-harvest cultural operations, maturity index at which the produce is harvested, methods of harvest, pre and post-harvest treatments, packing materials and storage methods. Postharvest technology can be defined as the techniques which help to reduce the post-harvest losses.

Assessment of harvesting stage

Climate may be related to periodical events in plants life. The science dealing with this relationship is termed as phenology. The phenological data for horticultural crops include dates of planting, germination, flowering, fruit set, ripening and harvest. These dates are determined by the climatic factors prior to as well at the time of harvest. A useful phonological concept for the prediction of the harvest time is based on degree days. A “ Degree day” can be defined as a day when mean daily temperature is one degree above the minimum temperature for the growth of the plant. The minimum temperature for the growth of a particular plant is termed as “zero temperature”. Zero temperature vary according to crop. E.g. if zero temperature of a particular crop is 50°F, then a day with mean temperature of 68°F would provide 18° days. The degree days are also called as “heat units”. It can be determined by how many degree days are needed for the horticultural crop to reach harvesting stage.

Plants grow in the open in nature, hence are subjected to biotic and abiotic stress.

Biotic stress is due to living components such as:

Insects and pests

Nematodes, mites, snails, slugs etc.

Vertebrates like rats, birds, jackal, bats etc.

The plant experiences biotic stress when root, stem, bark, shoots, leaves, buds, flowers and fruits are damaged by the influence and activity of biotic factors.

Biotic factors Diseases

Like the pests, diseases also cause stress to plants. Diseases are caused by fungus, bacteria and virus. Sometimes the exposed part of insect eaten plant part becomes the entry pathway for fungus, bacteria and virus.

Horticulture is an art and science of growing plants. It is the practice of engaging ailing people in various horticultural activities such as gardening and flower arrangements in order to improve their physical, mental and spiritual health. Research confirms that health benefits escalate manifold when people connect with nature and plants by viewing and/or interacting with them. Major benefits of horticultural therapy includes sensory simulation and mood enhancement, mental relaxation, improvement of memory and concentration and an overall feeling of wellness. Horticulture therapy also helps in cultivating positive emotions and provides a meditative mood. It eases both physical and emotional pains and also improves social skills. It is proven that a simple viewing of a garden benefits a person both physically and mentally.

This practice has been used as therapy for centuries. In 1798, Dr. Benjamin Rush, considered as the “Father of American Psychiatry” documented that gardening has positive effects on mentally ill patients. Following World War, II gardening was used as a form of occupational therapy for war veterans. The concept of using nature to improve human health began to gain credibility in 1970’s. Rachel and Stephen Kaplan (1989) found that natural environments had relaxing and restoration benefits for people. The Journal of Therapeutic Horticulture, a peer-reviewed publication of the American Horticultural Therapy Association, has published authentic research papers since 1986. The American Horticultural Therapy Association (AHTA) serves to promote and advance horticultural therapy as a therapeutic intervention and rehabilitative approach. Degrees and certificate programs in horticultural therapy are offered at several colleges and universities and information is available on AHTA website.

Horticultural therapy is a worldwide practice and recognized as an effective treatment for clients of all ages and abilities. It is being used in several diverse settings including hospitals, rehabilitation centres, youth outreach and recovery programs, nursing homes, senior care facilities, prisons, mental health facilities, schools, botanical gardens etc. The client is engaged in horticultural activities facilitated by some trained therapists to achieve specific and documented goals. AHTA believes that horticultural therapy is an active process that occurs in the context of an established treatment plan where the process itself is considered as the therapeutic activity rather than the end product.

Horticulture therapy is a growing profession in rehabilitation and addiction centers, vocational centers, youth mental health programs, hospitals and nursing homes, and old age homes or senior citizen centers, mental health centers, schools and colleges.

Flowers of the garden are brought indoors to enjoy the beauty. Fresh flowers are arranged in various styles according to one’s choice. There are two major and distinct styles of arranging fresh flowers, namely the European and the Japanese. Both the styles are greatly influenced by the place where they have been originated. Flowers are also dried and used for different arrangements. Fresh flowers are used as ornaments and also for making floral patterns on the floor called Rangoli.

A garden has a number of elements which give shape to its breath-taking view. Both animate and inanimate elements are included to achieve a beautiful garden The living elements are lawns, rock garden, water garden, sunken garden, hedges, edges, shrubs, topiary, flower bed, specimen plants, pergola and arches, while the inanimate elements, also called as adornments, are stones, road, footpaths, seats, lamps, lanterns, steps, bridges, huts, swings, fountain, cascade, bird bath, clock tower, umbrellas, sculptures etc.

Micro propagation in also called as in vitro propagation. In vitro is a Latin word, meaning ‘in glass’. It refers to procedures done in controlled artificial environment, outside a living organism. These procedures naturally occur in the living system. Opposite to in vitro is in vivo which is also a Latin word, meaning ‘in living’. Biological processes that occur within the living system.

Micro propagation refers to the multiplication of plants, in aseptic conditions in containers under controlled nutritional and environmental condition. Micropropagation is done in artificial growth medium from various plant parts like meristem tip, embryos, anthers, axillary buds, leaf section, shootlips etc. It is a method by which a true to type and disease free plant can be regenerated from a miniature piece of plant throughout the year. Some popularly grown crops from tissue culture are as follows

Ornamental plants - orchids, lilium, gerbera, cordyline, begonia, ficus, etc.

A kitchen garden/ nutrition garden is a vegetable garden where the vegetable crops are grown in the backyard of a house or any available space in compound or rooftop of the house, to meet the daily requirement of the family. All the members of the family are collectively involved in a kitchen garden.

For a balanced diet, 300 g of vegetables are to be included in our daily food. But on an average, Indians take only 160 g per day. Hence to fill up the gap, vegetable cultivation not only in farmers’ holdings but also in the home gardens is encouraged.

Organic cultivation is a deep rooted cultivation tradition, which had been practiced in many parts of the world, before the advent of the practice of use of chemicals.

After the green revolution there has been exponential usage of chemicals in agriculture, right from planting to harvesting. Chemicals are used in the form of nutrient inputs or for plant protection against pests, diseases, weeds and keeping deficiencies at bay. Nevertheless, after the awareness regarding the detrimental effect of chemicals on soil, climate and human health, there has been a lot of contemplation on substituting the chemicals with the organic alternatives. Growing crops using organic inputs is known as ‘organic cultivation’. Organic cultivation is a sustainable form of cultivation where a proper balance is maintained and management of plant nutrition, pests, weeds and diseases is done in a sustainable manner, keeping the soil microflora intact and active. It does not release any chemical gases in the atmosphere, does not make the soil acidic and the soil does not lose its structure and texture. Contrary to chemical farming, where over a time period, the soil quality, texture and structure is lost, in organic practices, the soil texture, quality and structure improves over time, the plants become strong and the quality of the produce is improved. Other than organic inputs, organic farming relies on some sustainable practices like using native germplasm, crop rotations with legumes, green manuring, mulching, inter cropping, mixed cropping, incorporation of farm and animal waste compost. These simple age old practices build to organic content in the soil and elevate the soil’s efficiency to make nutrients and water to the plants. The soil microflora and earthworms become biologically active, efficient and exponentially proliferate. The goal of organic cultivation is to get maximum produce from the soil in such a manner that the soil, air and water are clean and protected.

GAP stands for “Good Agricultural Practices” which are used for production of safe agricultural produce which has zero microbial load on it and inside it. It is important for the producer to be acquainted with GAP.

Good Agricultural Practice of horticultural crops are intended to apply to the growing and processing of all produce as traded and used in the European Union. Hence, they apply to the production of all plant materials used in the food, feed and medicinal industries. They also apply to all methods of production including organic production in accordance with the European Union regulations.

The farmers involved in the cultivation of horticultural produce should ensure that the there is no damage to the existing wild life and the biodiversity in the farms which are maintained. It is advisable to regulate wild gathering by a specific guideline.

The main aim of GAP guidelines is to ensure that the produce meets the demand of the consumer and is of high quality standard.

It is therefore, important that they are produced hygienically, in order to reduce microbiological load to a minimum. They are produced with care, so that negative effect on plants in the course of cultivation, processing and storage is limited.

All participants in the production process are required to comply with these guidelines voluntarily and to develop practical measures to achieve it.

Greenhouses are frames or inflated structures covered with a transparent or translucent material in which crops can be grown under partially controlled or totally controlled conditions of atleast. It is large enough to permit persons to work within, to carry out cultural operations. Greenhouse cultivation as well as other modes of controlled environment cultivation has been evolved to create favourable microclimate, for the crop production all through the year or during off season, when the produce is highly priced and remunerative. Green house is a broader term of protected structures which includes different types of structures according to the need. Green houses vary in size and complexity from small home or hobby structures to large commercial units, from solar green house to sophisticated environment controlled ones.

Plant growth regulators are defined as chemicals other than nutrients and vitamins which regulate (or) modify the physiological process ofthe plant. A plant hormones or phytohormones are chemical substances which are naturally produced by plant in specific organs and are translocated from site of synthesis to the site of action. They bring about specific physiological response, even in very low concentration. The quantity of synthesis of these hormones differ from plant to plant, plant internal and external factors. They are readily absorbed and these chemicals move rapidly through the tissues when applied to different parts of the plant. A plant growth hormone may be termed as a plant growth regulator, but not vice - versa, since other than natural plant hormones, plant growth regulators are synthetically manufactured chemicals.

Plant growth regulators have a diverse chemical composition. Based on the general response the chemical mediates in the plant system, they are classified as growthpromoters and growth inhibitors/retardants. However, a particular growth regulator may be growth promoter at a lower concentration and growth inhibitor at higher concentration.

The term growth refers to an irreversible increase in size and weight of the plant. It normally reflects an increase in protoplasm, which may occur through increase in size of the cell and number of cells. Hence, the process of cell elongation and cell division provides the basis for growth. Cell division and enlargement are complicated processes involving synthesis of many organic compounds such as protein, cellulose and nucleic acid, besides the physical force required, which results in cell enlargement. The growth of the whole plant is compartmentalized into areas called ‘meristem’ where cell division and cell elongation are localized. The meristem cells such as shoot apex, root apex, cambium and the intercalary zones undergo repeated mitosis resulting in replication of DNA and nuclear material and finally the cell mass increases. Adjacent to this area of meristematic activity is the region of cell elongation, which phases in to the region of differentiation and maturation.

The increase in protoplasm (occurring in cell division and elongation) is brought about through a series of events in which water, carbon - di-oxide and inorganic salts are transformed into living materials. These events include (a) photosynthesis (production of carbohydrates) (b) nutrient absorption and translocation (uptake of water, nitrogen and other mineral elements) and (3) anabolism (synthesis of various materials produced in plant from the carbon fragments produced by photosynthesis and from the inorganic nutrients and water absorbed from the soil). The energy required for all these processes is provided by a catabolic process viz., respiration (degradation of sugars and fats to release energy). The net balance of anabolism and catabolism results in growth of the plant.

The types of crops and the practices used to grow them and the sequence in which they are grown altogether refers to cropping system. It comprises of all components required for the crop production and their relationships with the environment. Traditionally the cropping systems aim at maximizing the per unit area productivity, which can be done by growing improved high yielding varieties, by adequate and scientific crop management practices and by increasing cropping intensities with the help of different cropping patterns.

In today’s world cropping system should produce more, at the same time the practices should be ecologically and environmentally sustainable. There should be efficient utilization of land and water. It should be designed to conserve soil, soil moisture and improve soil resilience.

Training and pruning of horticultural crops is very important practice to obtain a strong frame work of tree and sustained productivity. It is necessary to understand the various terms used to make the subject more comprehendible.

Crotch: It is the angle made by the scaffold limb to the trunk or the secondary branch to the scaffold limb.

Head: The point on the trunk from which first branch arises.

Leader: It is the main stem growing from ground level up to the tip dominating all other branches.

Low head trees: Trees in which scaffold branches arise within 60-70 cm height from the ground level.

High head trees: Trees in which the scaffold branch arise 120 cm above the trunk.

Scaffold branches: The main branches arising from the head are known as scaffold branches.

An orchard is the place where different kinds of fruit crops are grown in large scale. An orchard is a long-term investment and needs lot of planning and expertise. The orchard crops are generally trees, which ultimately have a large spread, so when they are planted, the distance between plants and rows is kept keeping in mind the final spread of the tree. So, before the trees attain their full growth, some suitable crops which are of short duration, like vegetables, flowers, spices and herbs are grown as intercrops.

Fruit trees are perennial, may be tropical, sub tropical and temperate types and thus require proper environmental conditions and suitable management practices for their successful growth.

Pot mixture is the media, used in the pots for propagation of nursery plants. It is the combination of different materials in a particular proportion to create better environment for rooting, growth and development of plants.

The most commonly used ingredients to make potting mixture areas follows