eChapters

Introduction

Minerals are present in all body tissues and fluids to carry out the physicochemical processes of life; both in animals and human. It is broadly classified into two categories: (1) macro and (ii) micro or trace elements. The macro elements include calcium, phosphorus, potassium, magnesium, sulfur, sodium and chloride, while elements such as iron, copper, cobalt, iodine, zinc, manganese, molybdenum, selenium and chromium come under micro elements. Animals need large amounts of calcium for construction and maintenance of bone and normal function of nerves and muscles. Phosphorus is a vital constituent of adenosine triphosphate (ATP) and nucleic acid and is also essential for acid-base balance, bone and tooth formation. Iron is an important component of the cytochromes for carrying out cellular respiration in addition to its vital role in red blood cells. Magnesium, copper, selenium, zinc, iron, manganese and molybdenum are important co-factors found in the structure of certain enzymes and are indispensable in numerous biochemical pathways. Animals need iodine to make thyroid hormones. Sodium, potassium and chlorine are important in the maintenance of osmotic balance between cells and the interstitial fluid. Excessive intake of some minerals can upset homeostatic balance and cause toxic side effects.

1. Introduction

In the last few decades there has been worldwide revival on the use of herbal drugs/phytochemical for diverse purposes including medicinal, nutritional and as cosmetic. The revival of interest in natural drugs and the herbal products was due to the widespread belief that ‘green’ medicine is healthier than synthetic products. This has led to the rapid spurt of demand for health products like herbal tea, ginseng and such products of traditional medicine during the 1980s. The health promotion and disease prevention strategy in treatment is widely prevalent in oriental systems, especially the Indian (‘Ayurveda’, ‘Siddha’, ‘Unani and ‘Amchi’) and the Chinese systems of medicine, which are finding increasing popularity and acceptance in the world over. Because of this sweeping ‘greenwave’ a large number of herbal drugs and the plant derived herbal products are sold in the health food shops all over the developed countries. Export– Import Bank reports revealed that the global trade of plant-derived and plant originated products is around US $60 billion (with growth of 7% per annum) where India holds stake of US $1 billion which is expected to reach 3 trillion US$ by the end of 2015 (Pandey et al. 2008). These targets can be achieved by providing scientifically validated, safe and standardized herbal products in domestic and international markets.

Standardization is an important step for the establishment of a consistent biological activity, a consistent chemical profile, or simply a quality assurance programme for production and manufacturing of herbal drugs. It is now well known that the pharmacological activity of a medicinal plants is due to the presence of certain bioactive phytochemicals, which are either primary or secondary metabolites. Amount of these compounds are controlled and conditioned by a variety of factors such as its genetic predisposition, habitat of the plant agro climatic conditions, season and also the stage of growth and development of the plant etc. Therefore, it is desirable to study the effect of these on secondary metabolites to get the right/optimum amount of secondary metabolites in the plant part/raw drug. The formation of herbal drug based on the traditional knowledge using modern tools is the requirement of the present day. WHO’s specific guidelines for the assessment of the safety, efficacy and quality of herbal medicines as a prerequisite for global harmonization are of utmost importance. Considering the importance of the herbal drugs/products, quality assurance of raw drug/formulation and developing botanical and chemical parameters for assuring their quality, have become major riders on the pharma or drug research and industry.

Objectives

1. To know the botanical and other names of spices and condiments.
2. To identify the family, plant habit and plant part used of spices and condiments.

Objective

To study the salient features of important varieties of spices and condiments.

1. Cardamom

Table 3.1: Released/Recommended Cardamom Selections- Estimated Yield and Source of Variability.

2. Black pepper

Table 3.2: Improved varieties of black pepper and their character

Introducton

The Working Group Report of Fifth Assessment Report 2013, by UN’s Inter governmental Panel on Climate Change (IPCC) says, that to limit warming to a rise of 2ºC above pre industrial levels, 1000 gigatones (trillion tons) is the upper limit of CO₂ that can be emitted. However, by 2011, we have already emitted 531 gigatones since last 260 yrs and in next 25 years, we will cross 1000 gigatones limit. There is a clear long-term global warming trend. Notably, the 20 warmest years have all occurred since 1981, and the 10 warmest have all occurred in the past 12 years.

Introduction
Forecasting of future weather conditions are based upon the collection and analysis of meteorological observations. With the help of advanced computing system and satellite data, the accuracy of weather forecasting is improving day by day. Weather forecasts may be classified according to the space and time scale of the predicted phenomena. With advanced technology, the spatial resolution of accurate forecast is enhanced and India Meteorological Department (IMD) has started to issue the Block-level forecast. The meteorological phenomenon can be distinguished among three classes of phenomena with a scale that is larger than the turbulent microscale: the mesoscale, synoptic scale, and planetary scale (Geer et al., 2018). Considering the variability of agro-climatic condition of India, the micro-level weather based agro-advisory has great importance to enhance the farm income. IMD has initiated the Block-level Agromet Advisory Services (AAS) in collaboration with Indian Council of Agricultural Research through establishment of District Agromet Units (DAMUs) at various Krishi Vigyan Kendra’s (KVK). Improvisation of the existing District-level Agromet Advisory Services (AAS) and starting of Block-level services are the basic objectives of DAMU. The District Agromet Units will act as nodal centre to prepare and disseminate the crop and locationspecific AAS to farmers at block level. Apart from this, the micro-level Agromet Advisory Service was implemented at NICRA (National Innovation on Climate Resilient Agriculture) villages through a network project of Indian  Council of Agricultural Research (Banerjee et al, 2018; Chakraborty et al., 2018). As a part of the nation-wide program, the feasibility of village level agromet advisory has been assessed. Thus, the importance of micro-level  agromet advisory and advisory on extreme weather events are discussed in this chapter.

The performance of plants is affected by the soil in which it grows and climate and micro climate of its surroundings.

Impact of soil

The soil provides anchorage, nutrients and water for plant growth. Soil fertility status, structure and texture influence the plant growth and productivity.

Classification of soil

On the basis of the organic and inorganic contents and particle size and texture, the soils have been classified. The inorganic matter in the soil constitutes of varying amount of sand, silt and clay, on the basis of which the soils are classified as follows.

Sandy soil

These soils have coarse textured and large pore spaces, have poor water holding capacity. Retention of nutrients in this soil is very poor. This type of soil is suitable for precocious crops. Sandy soils initiate rapid root growth, hence good as propagating medium. Since sandy soils has low in nutrient content, supplementation of adequate nutrition is imperative to achieve good plant growth.

Horticulture means the cultivation of garden plants. The garden plants are comprised of fruits, vegetables, ornamentals etc. The culture covers the operation from raising and planting of saplings to the ultimate use of the products and, as such, it is the most intensive of all the agricultural sciences. However, the concept of growing plants in garden or in an enclosure does not fit well in the changed perspective where large scale cultivation of fruits, vegetables and flowers extending over several square kilometers, in many a place nowadays, delimits the boundary of garden.

Importance of Horticulture

The importances of horticulture are established not only as a source of nutritious foods but also in many other ways which are narrated in the following points:

Social- Due to the long association, the fruits are seen to have a permanent seat in the social life of man. As a consequence, various fruits have acquired their place in art, music, literature, customs and habits of various nations of the world. The description of fruit as symbolic insertion in the poems and literatures, the use of whole fruit plant or part thereof in various social festive and public gathering, and conferring titles to individuals in the name of fruits or naming a region or whole district after the name of a fruit are nothing but an indication of their popularities and social position in various countries.

Horticulture is of immense importance for human beings. Among these major ones are nutritional importance, economic importance, medicinal values, environmental balance, aesthetic value and recreation. These are as follows.

1. Nutritional importance: Healthy body maintains a healthy mind. Human body requires vitamins, minerals, proteins, energy, fibers, etc. for its health. Fruits and vegetables are the chief sources of proteins, carbohydrates, fats, vitamins, minerals, organic acids, fibers, etc. Consumption of fruits and vegetables keeps the human body away from several illnessess and hence are recognized as protective foods; necessary for the maintenance of human health. As per the Indian Council of Medical Research; consumption of at least 120 g of fruits and 280-300 g of vegetables per day per capita is essential. Among vegetables, 80-100 g of leafy vegetables, 100-125 g of tubers as energy source and reamaining as other vegetables. However, the consumption of fruits per capita in India is still low. In countries like Italy, France and USA; the daily fruit consumption is 308, 232 and 223 g respectively. 

Introduction
Crop production is weather dependent and almost every crop has been affected by the changing climate. The favourable weather may result bumper production sometimes or even complete destruction of crop may take place under severe weather aberrations. The number of events of sudden aberrations is increasing in a cropping period with the gradual climate change in the recent past. It has been recorded too severe in case of perennial crops. Ready to harvest crops have several times been completely destroyed due to thunderstorm or cyclone in coastal regions or even in main land. Thus, Indian agriculture is being impacted by the climatic changes, and its affect is very much visible now (Aggarwal, 2008). Horticulture sector is not escaped from its’ ill affect and new challenges are coming for production (Singh et al., 2021). The role of adaptation strategy has now been felt crucial (Babu et al., 2021; Banerjee et al., 2022) and importance of agro advisory not only protects the crops from such types of risks but also provide guidance to farmers about the right time for activities in crop husbandry (Adak et al., 2018). Issuance for improving water productivity in water limited areas (Adak and Babu, 2024), micronutrient management for nutrient richness and enhanced yield (Adak et al., 2023; Shukla et al., 2020), rejuvenating unproductive orchards (Shukla et al., 2021; Mishra et al., 2007), disease (Misra et al., 2013; Shukla et al., 2015, 2019, 2020, 2021a,b; 2022; Kumari et al., 2021) and insect pest (Gundappa et al., 2016; 2018) management are becoming very crucial and essential for the benefit of growers.

Introduction

Medicinal Aromatic Plants (MAPs) play a valuable and important role in economic, social, cultural and ecological aspects of local communities the world over.

According to World Health Organization (WHO) “a medicinal plant is plant which, in one or more of its organs, contains substance that can be used for therapeutic purpose, or which are precursors for chemo- pharmaceutical semi-synthesis”.

However, a simplest definition of the medicinal plant would be “Medicinal plants are those plants which are used in official and various traditional systems of medicines throughout the world”.

Other definition could be “Medicinal plants are plants that provide people with medicines - to prevent disease, maintain health or cure ailments”.

In Indian traditions, all the plants in this earth are considered as medicinal [Jivak in Astanga Hriday (Sutra: 9-10)].

Aromatic plants are a special class of plants used for their aroma and flavour. Many of them are exclusively used also for medicinal purposes in aromatherapy as well as in various systems of medicine.

The term plantation crops refer to those crops which are cultivated on an extensive scale in contiguous area, owned and managed by an individual or a company.

There are two groups of plantation crops namely coconut, arecanut, oil palm, cocoa, cashewnut dealt by Ministry of Agriculture and tea, coffee, rubber dealt by the Ministry of Commerce.

These are high value commercial crops of greater economic importance and play a vital role in improving Indian economy, especially in view of their export potential, employment generation and poverty alleviation particularly in rural sector.

Proper handling, packaging, transportation and storage reduce the post harvest losses of fruits and vegetables. For every one percent reduction in loss will save 5 million tons of fruit and vegetable per year. Processing and preservation technology helps to save excess fruit and vegetable during the glut season (off season). The technology has become a necessity to improve the food safety and strengthen nation’s food security. The technology helps to boost export of agricultural commodities in the form of preserved and value added products e.g. mango, pineapple, citrus, grapes, tomatoes, peas, potato and cucumber being processed on a large scale.

Principles and Methods of Preservation of Fruits

Preservation: Preservation means just protect the foods against the spoilage, but scientifically it may be defined as a science which deals with the process for prevention of decay or spoilage of the food is called preservation. In other words, just controlling the physical, chemical or microbial changes in the foods is called preservation.

• The term Spice and Condiments applies to natural plant or vegetable products or mixtures in whole or ground form, which are used for imparting flavour, aroma and piquancy to the food items.

• Under the act of Parliament, a total of 52 spices are brought under the purview of Spices Board.

• However 109 spices are notified in the ISO list.

• India is the largest producer, consumer and exporter of spices and spice products in the world and produces more than 50 spices.

• The spices production in India is of the order of 5.74 million MT from an area of about 3.07 million ha.

• Chilli is the major spice crop occupying about 25.8 percent of area under cultivation and contributing about 22.7 percent of total spices production in the country.

• Garlic accounts for 21.9% of production and 8.1% of area Turmeric accounts for 16.9% of production and 6.3% of area.

• Rajasthan occupies major area under spices owing to seed spices cultivation in the state followed by Gujarat, Andhra Pradesh, Madhya Pradesh and Karnataka.

• Andhra Pradesh, which is the foremost state producing chilli and turmeric in the country, ranks first in terms of production.

The arid zone eco-system of Rajasthan is very fragile and is prone to serious imbalance even with the slightest disturbance due to mismanagement of resources. Among the eastern margin of the mean annual rainfall is 500 mm, while in the western most part it is 100 mm. The rainfall is monsoon driven. It comes between June and September (in 9-21 short spells). Large coefficient of variation (40-60%) and erratic distribution during the monsoon are characteristics features of the rainfall, leading often to prolong drought and failure of rainfed crops (More and Samadia, 2007). Temperature as low as 4.4° C, and as high as 50° C has been recorded in Western Rajasthan. Farming system and cropping system approach for sustainable use of farm resources and reduced risks have been successfully demonstrated in perennial horticulture. There is great pressure of livestock on available feed and fodder, as land available for fodder production has been decreasing. The requirement of dry and green fodder by 2025 would be 1170 mt and 650 mt while supply is expected around 411.3 mt and 488 mt deficit will be 64.87 % green fodder and 24.92 % dry fodder (Table 1).

Abscission: The shedding of leaves, flowers or fruits as a result of formation of layer of loosely adhering cells at base that breaks apart readily.

Accent: It is created in the garden to avoid the monotonous view. Mostly unusual object like tall fountain, tree, statue etc are used to create the effect

Acre: A unit of measurement of land area equivalent to 4840 square yards or 0.4047 hactare.

Acropetal: Developing sequentially from basal to the apical position.

Acrylic: It is a glassy thermoplastic that is incorporated in rigid plastic to enhance wither ability.

Aestivation: The use of high temperature to promote flowering.

After-ripening: Physiological changes that take place within a dormant seed or bud during dormancy or It is a type of embryo maturation which enables germination of seeds.

India, internationally recognized as the principal producer, consumer and exporter of spices globally, is often referred to as the spice bowl of the world. A total of 75 spices out of 109 are cultivated in the country, among which
pepper, cardamom, chillies, ginger, turmeric, coriander, cumin, and fenugreek are of significant importance. The approximate export of spices during 2021- 22 was 15,31,154 MT valued at 30,576.44 crore (US $4,102.29 million)
compared to 17,58,985 MT valued at 30,973.32 crore (US $ 4,178.80 million) during the previous financial year. Indian spices are exported to more than 180 destinations, with the leading importers being USA, China, Bangladesh, Thailand, UAE, Sri Lanka, Malaysia, UK, Indonesia, and Germany. The key spice-producing states of India include Himachal Pradesh (ginger), Haryana (garlic), Karnataka (pepper, ginger, cardamom (small), chilli, turmeric and garlic), Orissa (ginger, chilli, turmeric, and garlic), Kerala (pepper, chilli, turmeric, nutmeg, mace, and clove) and Rajasthan (coriander, chilli, cumin, garlic, fenugreek, fennel, and ajwain).
Seed Spices in Rajasthan Economy
Of the 75 spices grown in India, 20 are classified as seed spices, where the economically important part is the seed.

India is emerging as a major stakeholder in the global horticulture scenario accounting for 10.00 % of the world production of fruits. This has been made possible due to concerted efforts of scientists and progressive farming community. In recent years, there is considerable awareness about the nutritional security and food safety. Underutilized fruit trees not only provide nutrition and income security to the people but can also ameliorate the harsh environment of the semi-arid and arid region. Jamun has been named as diabetes fighter for its hypoglycemic (lowering blood sugar) properties.The minor fruits like bael, karonda, khirni, mahua, tamarind, mulberry, pilu, chironji, phalsa and wood apple have tremendous scope for their proper utilization. These fruits are nutritionally rich in bioactive compounds and some of these are of great medicinal value, processing quality and other uses. They have a growing demand in local as well as export market and for processing besides diversification in agriculture in the wake of global changes in weather conditions.

ABSTRACT

Commercially valuable plants are one of the main sources of economy. Growth, development and yield of these plants are severely affected by various environmental factors, which depend on dynamic fluctuations in different parameters. To counteract these responses, plants try to adapt evolutionarily but one form of adaptation is never going to suffice the simultaneous attack of multiple stresses. Crossing and plant breeding approaches came to practice, which is quite beneficial but requires lot of screening and is manpower consuming. In recent era new approaches have been developed combining recombinant DNA technology with tissue culture techniques to obtain improved varieties with specific desired traits. This method had an edge over other technologies but it still carries lot of complications. It is evident that genes do not act alone but act cooperatively and remain in continuous influence of other factors. So to target a particular gene for improved variety development requires a lot of detailed micro-details. In this communication, an attempt have been made to discuss mainly how transcription factors (TFs) have been targeted for this purpose. TFs in many occasions act as master regulators or central players of a particular pathway. This is attained by their ability to interact with multiple cis-elements present in the promoters of downstream stress or metabolism responsive genes. This behavior sometime leads to occurrence of some unwanted traits, which should be kept in mind to develop transgenics. Here, we focused mainly on important TF families such as ERF, WRKY, NAC and bHLH. We have been an attempt to analyzed how these TFs influence growth, yield and stress response of commercially valuable plants such as rice, wheat, tomato and cotton. This section will help to understand the genetic manipulation of plants can be targeted for improved variety development.

Introduction

The semiarid and arid ecosystem is not only neglected in India but also the whole world in respect of socio-economic and scientific development. Middlefon and Thomas, 1997) reported the arid and semi arid covers 47% of land surface of the plant and home town to 1/3 of human population. Conventional plant breeding so far only increased the yield of crops grown under arid tropics at about half the rate achieved for crops grown in temperate regions (Turner, 2004, b). The major crop of other ecosystem are considered as under exploited crop of arid ecosystem. Tomato (Lycopersicon esculentum Mill.), is one such crop, which was domesticated in ancient Peru, has become the most popular and widely consumed vegetable in the world today, for its rich flavor and high nutritional value (Vitamins A and C)

An overwhelming proportion of plant material used for compounding herbal medicines and nutraceuticals is currently collected from the wild. With traditional health care systems gaining increasing popularity, the enormous amount of plant material extracted from the limited plant populations available in natural stands is a cause of justifiable alarm. The unscrupulous plunder of plant populations growing in the wild is pushing increasing number of important species to the endangered category and many to the verge of extinction. Also, unethical practices like adulteration and storage of medicinal plants under unacceptable conditions are rampant. All this is happening, when the consumer is becoming increasingly quality conscious and is demanding traceability of source material used for formulations and adherence to good practices for the entire chain of manufacturing process for herbal drugs and plant-based nutraceuticals. Clearly, solution to this problem lies in replacing the ‘collection from the wild’ paradigm to ‘production by cultivation’. An essential prerequisite for meeting this objective is a well thought out research agenda which not only tackles productivity enhancement goals for the targeted plant species but also addresses the validity of the entrenched notion among healers who use the traditional medical systems, that only material collected from the wild produces the desired results. The reason advanced in support of this argument is that phytomedicines are derived from plant populations growing in the wild under organic conditions and under their natural rhizosphere and rhizoplane. The associated soil-microbial complexes, and a variety of endophytic microorganisms, may be the initiators or modifiers of the production and expression of medicinal properties.

An overwhelming proportion of plant material used for compounding herbal medicines and nutraceuticals is currently collected from the wild. With traditional health care systems gaining increasing popularity, the enormous amount of plant material extracted from the limited plant populations available in natural stands is a cause of justifiable alarm. The unscrupulous plunder of plant populations growing in the wild is pushing increasing number of important species to the endangered category and many to the verge of extinction. Also, unethical practices like adulteration and storage of medicinal plants under unacceptable conditions are rampant. All this is happening, when the consumer is becoming increasingly quality conscious and is demanding traceability of source material used for formulations and adherence to good practices for the entire chain of manufacturing process for herbal drugs and plant-based nutraceuticals. Clearly, solution to this problem lies in replacing the ‘collection from the wild ’ paradigm to ‘production by cultivation ’. An essential prerequisite for meeting this objective is a well thought out research agenda which not only tackles productivity enhancement goals for the targeted plant species but also addresses the validity of the entrenched notion among healers who use the traditional medical systems, that only material collected from the wild produces the desired results. The reason advanced in support of this argument is that phytomedicines are derived from plant populations growing in the wild under organic conditions and under their natural rhizosphere and rhizoplane. The associated soil-microbial complexes, and a variety of endophytic microorganisms, may be the initiators or modifiers of the production and expression of medicinal properties.

It also so happens that a majority of medicinal plants are shade loving, C4 types and will necessarily require their species-specific ambient environment for elicitation of the medicinally relevant moities.

ABSTRACT

Successful in vitro clonal propagation of mature tree of Lasoda (Cordia myxa Roxb) has been achieved through single node explant. Both types of organogenesis of shoot and root formation were obtained by placing nodal explant on shoot induction medium and subsequently on rooting medium. More than 90% culture were responsive for in-vitro axillary shoot proliferation on MS media supplemented with 30g Sucrose 8.0 g agar and a combination of 2.0 mg BA and 0.1 mg NAA per litre. After culture period of 6-8 week, the nodal explants with micro shoot were rooted on the MS media supplemented with30 g Sucrose + 3g activated charcol and different level of NAA (0, 1, 3 and 6 mg/ litre). The season and position of explant on the mother plant was also shown to influence in vitro performance of the regeneration in terms of establishment of aseptic culture, percentage of responsive explants and intensity of leaching of growth inhibitory phenolic chemicals.

ABSTRACT

Medicinal plants are the most important source of life saving drugs for the majority of the world’s population. Therefore the biotechnological tools are important to select, multiply and conserve the critical genotypes of medicinal plants. With the increase in the demand for the crude drugs, the plants are being overexploited, threatening the survival of many rare species. Also, many medicinal plant species are disappearing at an alarming rate due to rapid agricultural and urban development, uncontrolled deforestation, and indiscriminate collection. Advanced biotechnological methods of culturing plant cells and tissues should provide new means for conserving and rapidly propagating valuable, rare, and endangered medicinal plants. Maintenance of germplasm in the green house or in the field has many difficulties and so there is a need for the in vitro conservation of these plant germplasm. In the present paper we report the in vitro conservation of two medicinal plants and each of it has its own medicinal value. The tuberous roots of Holostemma are medicinally important and are utilized as a major ingredient of the drug ‘jivanthi’. Tylophora is used as a herbal medicine and the plant has been traditionally used as a folk remedy for the treatment of bronchial asthma, bronchitis and dermatitis. Shoot buds of these plants were cultured in Murashige and Skoog’s (1962) medium with different strengths and with different concentrations of sucrose and mannitol. The different treatments induced varied response with respect to the slowing of growth of cultures. Sucrose was found essential in combination with mannitol to induce maximum reduction in the growth of the plant tissues. The cultures could be maintained without sub culturing for over one year and resumed normal growth on transfer to mannitol free medium. This method of conservation has many advantages.

ABSTRACT

Jasmine is one of the economically most important flower crops cultivated for its fragrant flowers and valuable essential oil. In vitro studies to explore the possibility of regeneration of plantlets from different explants and to undertake biochemical analysis to detect the flavour compounds if present in the in vitro cultures were taken up with local varieties of Jasminum grandiflorum.

Among the different explants tried, leaf bits were found ideal for callus induction, followed by petals and shoot tips. MS medium with 2, 4-D at 1.25 ppm was the suitable medium for callus induction in leaf explants. Miller medium with NAA at 1.25 ppm and BAP at 1.25 ppm was the suitable medium for callus induction in petal explants collected from 18 days old buds of local varieties of Jasminum grandiflorum. Among the different media combinations tried, MS medium supplemented with 0.2 ppm NAA and 0.3 ppm GA₂ is the best medium for callus induction in shoot apex explants of 10mm size. Direct regeneration of plants was obtained in 10 per cent of shoot apex explants cultured in MS medium supplemented with 0.2 ppm GA₁ and 3 ppm BAP in 60 days. A maximum of 95 per cent embryogenicity is induced in leaf callus transferred to MS basal medium supplemented with 1.5 per cent sucrose from MS medium with 2, 4-D at 1.0 ppm.

The peroxidase assay indicated that embryogenic calli had six isoperoxidases while the non-embryogenic had only two. Biochemical analysis of the callus samples showed high Phenylalanine ammonia lyase and phenol indicating the initiation of phenolic pathway, which branches out and ultimately leads to the synthesis of flavour compounds in callus.

ABSTRACT

An efficient in vitro micro propagation protocol was developed for direct shoot produc tion of Contoloupe (Cucumis melo variety (NS Abhijit) using cotyledons as explant. The excised cotyledons were cultured on Murashige and Skoog’s medium (MS) contain- ing plant growth regulator, 6 Benzyl adenine with various concentrations for the study of shoot production. The best condition for the shoot production was with 1.2mg/l Benzyl adenine (BA) in MS medium. The shooting frequency was 65% and three shoots were obtained from each explant after 14 days of culture. The shoots (~1.5 cm length) were rooted most effectively on 0.1mg/l of α Naphthalene acetic acid (NAA) supplemented MS medium. We have also standardized the transformation protocol for GUS gene in the muskmelon variety Eden Gem to see test the feasibility of this variety for Agrobacterium mediated transformation. The transformed explants were confirmed through Indigogenic GUS assay.

ABSTRACT

The investigation was carried out to develop protocol for micropropagation of acid lime (Citrus aurantifolia S) var. Sai-Sharbati. Shoot tip and nodal segment explants were obtained from axenically grown seedlings having 21 to 30 days age. Direct regeneration of shoots, roots and whole plant without intervention of callus was obtained from both the explants on MS basal media. In both the explants 100 per cent shoot proliferation was induced in nutrient medium MS + BA 0.25 mg/l + ME 200 mg/l. The shoot proliferation rate was more (5.0) in nodal segment as compare to the shoot tip explant (4.33). Regenerated shoots of more than 2.5 cm length with minimum of 4 leaves were transferred for rooting. Half strength MS medium supplemented with IBA 1.0 mg/l was best for root induction and root initiation within 15.66 days. More than 75 per cent plantlets were survived after hardening period of four weeks.

ABSTRACT

Rubia cordifolia is an effective blood purifier in indigenous medicine. Habitat destruction and indescriminate harvesting is posing threat to its survival necessitating concerted efforts for conservation. The present study is an attempt to standardise in vitro multiplication protocol, a basic step for in vitro conservation, in Rubia cordifolia. Nodal explants cultured in MS basal medium supplemented with BA 1mg l ^-1 and IAA 0.2 mgl^-1 induced axillary bud break. The in vitro formed shoots developed roots on MS basal medium supplemented with IBA 1mg l ^-1. The plantlets were ready for plant out in 100 days of culture initiation and established well on planting out.

ABSTRACT

Plants as a source of medicine are of special importance in the countries like India which has well developed traditional system of medicine which derive more than 90 per cent of medicaments from higher plants. Several of these also constitute important ingredients of Allopathic medicines. Most of the requirements for the medicinal plants are met from wild sources. But due to the changing ecological factors in the forests and unserupulous collection of medicinal plants, the wealth of medicinal plants is getting depleted and some types have already become extinct. The collection of these plants from the forests cannot cope up with the ever increasing and changing demand from the pharmaceutical, perfumery and cosmetic industry. In order to provide regular and sustained supply of medicinal plants, it is essential now to domesticate and systematically cultivate these plants for which large scale availability of planting material has to be ensured. In this context, in vitro multiplication protocols were standardised for rare, valuable and endangered medicinal plants such as Holostemma ada-kodien, Trichopus zylanicus, Aristolichia indica and Terminalia chebula. The study indicates the feasiblity of rapid multiplication of these plant species using in vitro techniques and the standardised protocols could be used to generate large number of true to type protocols within a short time.

ABSTRACT

Kattupadavalam (Trichosanthes cucumerina) is regarded as a laxative and blood purifier, considered beneficial in the treatment of skin diseases. Its entire supply being met from wild, unscrupulous exploitation has led to erosion of genetic resources of this crop. Hence it is imperative that in vitro trechniques are employed to conserve this valuable medicinal species and to evolve feasible protocols for large scale multiplication of this crop. Nodal cultures were established from two months old seedlings of Trichosanthes cucumerina and Murashige and Skoog (MS) medium, supplemented with BA @ 0.5 mg l^-1. Subculture of initiated shoots in the same medium at intervals of three weeks registered enhanced release of axillary buds producing 21 shoots from a single culture at the end of fifth subculture cycle. Elongated shoots were rooted in half strength MS medium, following a pulse treatment with IBA @ 1000 mg l^-1. A single stage root hardening process, wherein, rooted plantlets were subsequently transferred to half strength semisolid MS medium supplemented with 2 per cent sucrose, improved success rate. The rooted plantlets planted out in sterilized media and subjected to a hardening process in a phased manner, survived well.

ABSTRACT

Mucuna pruriens (L.) DC. is one of the important medicinal plants that contains L DOPA (L-3,4 dihydroxy phenylalanine) which is the precussor for the neurotransmitter dopamine that plays an important role in curing Parkinson?s disease. To accommodate the huge demand for L DOPA, in vitro production of the drug using cell cultures is felt essential. Hence, an experiment was carried out with completely randomized design with seven treatments viz., T₀ - Control; T₁ - MS + 2 mg l^-1 NAA; T₂ - MS + 2 mg l^-1 IAA; T₃ - MS + 2 mg l^-1 2,4-D; T₄ - MS + 2 mg l^-1 Kinetin; T₅ - MS + 2 mg l^-1 BA; T₆ - MS + 1 mg l^-1 IAA + 1 mg l^-1 BA and 3 replications. Among the various explants investigated stem bits registered the least days (12.38) for callus induction. The stem bits responded well with better callusability (78.57 per cent), relative growth rate (2.57) and callus index (249.16). MS medium + 2 mg l^-1 2,4-D was found to be the best for inducing callus in various explants namely stem bits, leaf bits and root bits. L DOPA accumulation was more in cell suspension cultured in liquid MS medium with 4 per cent sucrose + 1 mg l^-1 IAA + 1 mg l^-1 BA and that was confirmed by means of HPLC.

Introduction

Plants contain a variety of substances of nutraceuticals importance which are useful medicines, food additives, cosmetics and health supplements etc. However, low availability under in vivo condition, decreased plant resources, increases in labour cost and other problems in obtaining these high-value added substances from natural plants have pointed toward the use of in vitro techniques for production of the products. Because in vitro cultures are not affected as the production affected under in vivo conditios.This has led scientists and biotechnologists to consider plant cell, tissue and organ cultures as an alternative way to produce the corresponding secondary metabolites.Therefore, studies on the production of useful metabolite by in vitro techniques have been carried out on an increasing scale since the end of the 1950’s. The large scale cultivation of tobacco and various vegetable cells was examined in the late 1950’s and early 1960’s in the U.S., Canada, and Europe. At present, several firms are manufacturing a plant pigment, shikonin, and ginseng cell biomass on a commercial scale and several other products including anti-oxidant products.

Research efforts on in vitro derived extracts is well documented on each of these major product categories: flavors (onion and garlic, peppermint and spearmint, fruit flavors, chocolate aroma, seaweed flavors, vanilla, celery, coffee, spice, sweeteners, and so on); edible colors for foods and medicines (mainly betalains and anthocyanins): non-food pigments for cosmetics and textiles (shikonin, berberine, and various other products); several examples of fragrances and essential oils; and bioactive natural insecticides and phytoalexins useful in current integrated pest management programs. Of course, intensive activity has centered on production of natural drugs of chemoprotective compounds from plant cell culture. Some of the most prominent pharmaceutical products in this latter category include ajmalicine (a drug for circulatory problems) from Catharanthus roseus and taxol (a phytochemical effective in treatment of ovarian cancer) from Taxus species.

ABSTRACT

Aprotocol for in vitro clonal multiplication of Dendrobium var. Betty Ho is reported. Aseptic shoot tip explants cultured in MS medium containing Benzyl Adenine (5 mg l^-1) and casein hydrolysate (1000 mg l^-1) recorded a maximum multiplication rate of 10 fold in 30 days. Explant cultured in LS) medium supplemented with 1-napthalene acetic acid (0.1 mg l^-1) and Kinetinetin (1 mg l^-1) developed a maximum of 8.3 shoots in 30 days. Shoots thus obtained were rooted on MS medium supplemented with BA (0. 1mg l^-1 ) and NAA (0.5mg l^-1). Rooted plantlets after hardening were transferred to green house.

ABSTRACT

In vitro clonal propagation of Smilax zeylanica Linn was attempted. Axillary buds from shoot tip and single node explants were induced to proliferate on half strength MS basal medium supplemented with BA (0.5, 1.0 and 2.0 mg l^-1) and IAA (1.0 and 2.0 mg l^-1). Multiple shoots (6.0 - 10.0 per explant) developed from the axillary buds in about four weeks time in all the treatments tried. However, the highest rate of multiple shoot production was in the medium containing BA 1.0 mg l^-1 and IAA 1.0 mg l^-1. Maximum shoot length was also observed in the same medium. Rooting (83.3 per cent) of micro shoots could be induced on MS supplemented with NAA 0.5 mg l^-1. Individual rooted plantlets could be successfully transferred to soil and established in the field with 80 per cent survival.

Introduction

At the Banana Research Station, Kannara activities focused on Musa germplasm conservation and evaluation are in progress. The aim of the project is to collect and conserve wild and cultivated germplasm representative of the diversity existing in the Musa genepool for evaluation and subsequent use in breeding programmes and to make available selected superior material to the farmers of Kerala. The center presently holds a Musa germplasm of 256 accessions in its field gene bank. These accessions have been characterized and evaluated as per INIBAP/IPGRI Musa descriptors and a database developed.

In a vegetatively propagated crop like banana, in vitro culture is widely employed in germplasm introduction and conservation and is an essential requirement for the safe movement of germplasm (Sharrock and Engels, 1996). By this method, germplasm material is readily propagated, thereby facilitating the dissemination of disease free plants. Since 2001, efforts have been made at Banana Research Station, Kannara to introduce forty new exotic accessions employing in vitro culture for conservation and evaluation. These are material from the global Musa germplasm collection maintained by International Network for the Improvement of Banana and Plantain (INIBAP), France and supplied by National Bureau of Plant Genetic Resources (NBPGR), New Delhi.

ABSTRACT

Coleus forskohlii Briq. is a threatened medicinal plant , susceptible to fungal disease. The regeneration of this plant has been achieved on MSo medium supplemented with 2 mg/l or 3 mg/l BAP and 0.2 mg/l 2,4-D for callus induction using leaf as explants. Multiple shoot production from the leaf-derived transformed callus of coleus has been standardized using the various concentration of NAA with 2 mg/l BAP. The combination of 2 mg/l BAP and 0.5 mg/l NAA was found to be best for multiple shoot production. Rooting was found to be best on MS medium supplemented with 0.7 mg/l NAA. Coleus forskohlii was transformed with glucanase-chitinase gene along with npt-II gene. The presence of npt-II genes in the leaves of putative transformants were confirmed by PCR analysis. SDS- PAGE analysis and glucanase assay revealed the over expression of glucanase enzyme in the transgenic plants. The transgenic coleus plants survived the infection caused by Fusarium chlamydosporium due to the accumulation of glucanase-chitinase enzyme, where as control plants were susceptible to the disease.

ABSTRACT

Aprotocol has been developed for the induction, maturation and germination of somatic embryos from leaf explants of Hevea brasiliensis (clone RRII 105). Leaf explants were cultured with their adaxial sides on modified Murashige and Skoog (MS) medium supplemented with different combinations of phytohormones such as 2,4-D & BA, NAA & BA as well as 2,4-D, BA and NAA. Compact calli could be developed from the cut ends of the explants on media containing 2,4-D (1.5 mg/l) and BA (1.0 mg/l) whereas pale yellow friable calli was obtained on media which contained NAA (0.2 mg/l) along with 2,4-D (1.2 mg/l) and BA (1.0 mg/l). The calli formed were detached from the explants and sub cultured for proliferation in medium containing reduced auxin (0.4 mg/l 2,4-D) and slightly increased level of sucrose (40 g/l). Proliferated calli were then sub cultured for embryo induction in modified MS medium supplemented with different auxins and cytokinins. Embryo induction could be achieved in modified MS medium containing BA (2.0 mg/l), GA (1.0 mg/l) and NAA (0.2 mg/l) and maturation occurred in WPM medium containing BA (0.3 mg/l), TDZ (0.5 mg/l) and GA (1.5mg/l). The cotyledonary stage embryos developed into plantlets on transfer to hormone free ½ MS medium. A distinct feature of this study is the induction of somatic embryogenesis from leaf explants of Hevea which has not been reported previously. This explant could be used further as an ideal target tissue for Agrobacterium mediated genetic transformation in Hevea.

ABSTRACT

In cocoa (Theobroma cacao L.) there are successful reports on the production of shoots from nodal segments under in vitro,the rooting was always inconsistent and mortality during hardening stage was very high. In the present study 20-30 per cent rooting was obtained in half strength MS supplemented with activated charcoal. Maximum rooting was obtained when the shoots were pretreated with IBA 5000 mg l^-1 for three seconds followed by culturing in the media.

ABSTRACT

Aquilaria agallocha Roxb. (Thymelaeceae), otherwise known as ‘Eagle wood’ is a large evergreen tree restricted to the eastern parts of India and Bhutan. The tree is valuable for its fragrant wood - ‘Agar wood’ which is tremendously used in the perfumery and incense stick industries. Lack of rapid natural regeneration and over exploitation has reduced its population drastically. Micropropagation offers a rapid means for clonal multiplication and conservation of this endangered species and attempts were made to standardize available protocol for in vitro multiplication. Media and conditions were standardized for culture establishment and propagule multiplication. Multiple shoots were induced in vitro from nodal segments of 10 yr old trees having enhanced axillary branching. Culture establishmnet was strongly influenced by the nature of the explant and the season of collecting the explants. Nodal segments collected during the period March- April from current season shoots showed a better response (90 %) in half strength Murashige and Skoog medium supplemented with 1.0 mg l^-1benzyl adenine and 0.1 mg l^-1kinetin. The rudimentary multiple shoots induced, proliferated in the basal media supplemented with benzyl adenine (0.1 mg l^-1), naphthalene acetic acid (0.5 mg l^-1) and coconut water (10%). This media also supported further multiplication in serial subculturing with a multiplication rate of 1:6. There exists no earlier report on in vitro multiplication of this tree species valued in perfumery.

ABSTRACT

Ipomoea mauritiana (L.) belonging to the Family Convolvulaceae is an important medicinal plant. The roots, leaves and flowers possess medicinal properties. It is used in indigenous medicine as a tonic, aphrodisiac, galactogogue, diuretic, carminative, expectorant and appetizer. It is useful in syphilis, inflammation, leprosy, diseases of blood and in the improvement of voice and complexion. Tender nodal portions from one year old disease free plants grown in pots were used as the source of explants. The explants were inoculated on half strength MS medium containing 3.0 per cent sucrose and 0.6 per cent agar supplemented with 2,4-D 0.5 mg l^-1. All the explants showed callus initiation after three weeks. The contamination free, one week old callus cultures were used for testing the effect of the different treatments. The treatments comprised of different combinations of BA (0, 1.0, 2.0, 4.0 and 8.0 mg l^-1) and NAA (0 and 0.1 mg l^-1) added to half strength MS medium. The highest rate of shoot regeneration was obtained on half strength MS medium supplemented with BA 4.0 mg l^-1. Rooting of the proliferated shoots could be induced on the same basal medium supplemented with IBA 1.0 mg l^-1 and NAA 1.0 mg l^-1. The regenerated plants were hardened in a humidity chamber and successfully transferred to pots, with 90 per cent survival.

ABSTRACT

Centella asiatica (Linn.) previously Hydrocotyle asiatica belongs to the family Apiaceae.It is commonly called gotu kola and is rich in triterpene glycosides such as asiaticoside and madecassoside. Health claims range from enhanced memory and longevity, to the treatment of leprosy. It is also used in the treatment of various skin diseases, ulceration, chronic rheumatism, malaria, fever, epilepsy and enlargement of glands. Inspite of its wide range of uses,it is still collected from the wild and commercial cultivation is lacking. A good tissue culture protocol therefore would be useful for commercial propagation and thereby saving the plant from extinction. Of the various explants, nodal segment gave the best response. For direct shoot regeneration from nodal segments, BAP 2.0 mgl^-1, and BAP 1.0 mgl^-1 with Kinetin 1.0 mgl^-1 was found optimum. Callusing was observed in nodal segments and leaf bits when 3.0 mgl^-1 NAA along with 0.3 mgl^-1 BAP was used. ½ MS medium supplemented with IBA 0.5 mgl^-1 and NAA 0.2 mgl^-1 induced rhizogenesis of the regenerated shoots. Rooted plantlets survived well in a combination of pot mixture (1 sand: 1 soil: 1 FYM) and Vermiculite in the ratio of 1: 1.

Incentives are a powerful stimulus to transfer of technology. They can encourage researchers to get their results to market, a new career path for many of them. They can create a culture that attracts and retains entrepreneurial staff, scientists, students and technology transfer professionals (TTPs) that view the university as a career path and a vehicle for making a contribution to society. Working in a pro-entrepreneurship team of colleagues can also lead to getting people to think in terms of making a connection between their work and its application. Incentives can be short-term or long-term in nature, and can be tailored to individual or collective requirements. As it is likely to be challenging for universities to design incentives that cater to every researcher’s or TTP’s desire, they can design incentive programs to cater to various aspects of human. Nature.
12.1 Why incentives matter in this ecosystem
Incentives can be either financial and non-financial. These are needed to ensure that researchers and TTPs remain motivated, aligned with institutional goals, and committed to the long-term process of technology transfer and commercialization. Incentive programs serve several important purposes:
• Motivation: Incentives energize individuals to pursue innovation and commercialization, often over long- and uncertain-time horizons.
• Retention: By offering recognition, rewards, and career advancement opportunities, institutions can retain high-performing researchers and skilled TTPs.
• Alignment: Well-designed incentives ensure that the personal goals of stakeholders align with institutional and societal objectives.

Morphological characteristics

A. balfourii Stapf is an erect, glabrous shrub that grows upto 1.5 m in height. The plant has branched stems which are dull purplish brown in colur that reach about 1.2 m high. Lower leaves are long stalked while the upper ones are short. The plant has long heavy tubers with 7 to 12 cm in length. Roots are tuberous and fusiform.

Floral characteristics

The plants bears about 29 cm long inflorescence with numerous flowered racemes along with the yellow tomentum. The flowers are hermaphrodite and the mode of pollination is by bees. Sepals are helmet shaped, pubescent and blue in colur. The upper two petals are hooded and enveloped in the helmet, and the below two are glabrous. The stamens are many, the filaments are hispidulous, and the pollen is three-colpate. Carpals are yellowish, 3 to 5 in number and tomentose. Follicles range in size from 2 to 5, are sessile, and silky pubescent. Along the raphe, the seeds have a broad winged appearance. The months of September to November are ideal for flowering and fruiting in A. balfourii.

Indian Council of Agricultural Research; formerly known as Imperial Council of Agricultural Research, established on 16^th July 1929 and located at New Delhi. It is an autonomous organization under Department of Agricultural Research and Education (DARE), Ministry of Agriculture and Farmers Welfare, Government of India. It is the apex body for coordinating, guiding and managing research and education in agriculture including horticulture, fisheries and animal sciences. This is one of the largest national agricultural systems in the world.

The ICAR has played a pioneering role in ushering in the green revolution and subsequent developments in agriculture through its research and technology development. Thus, the food grains production increased by 5.6 times, horticultural crops by 10.5 times, fish by 16.8 times, milk by 10.4 times and eggs by 52.9 times to achievenational food and nutritional security during the period of 66 years (1950-51 to 2017-18). It has also played a major role in promoting excellence in higher education in agriculture. Organizational setup of ICAR to achieve mandate is as follows.

5.1. Origin and distribution

• True or black mustard: Brassica nigra Koch-Self sterile

• White mustard (Yellow mustard) : Brassica compestris, Syn: Sinapsis alba- Self sterile

• Indian or brown mustard: Brassica juncea (L.) Czern

• These three species are commonly used as condiment.

• White mustard is indigenous to Southern Europe, whereas brown mustard is from China introduced to Northern India.

• Black mustard is endemic in Southern Mediterranean region.

• White mustard is widely cultivated in Australia, China, Chili, Denmark, Italy, Japan, UK, Netherlands, North Africa, Canada and USA.

Morinda citrifolia L is a unique horticultural plant belonging to Rubiaceae family. It has a tremendous demand because of its high medicinal value. Increase in demand has led to commercial farming of the tree. Noni is an ideal plant for mixed farming with other horticultural plantations. There is concurrent efforts to select early bearing plants with big size and fruits.  Noni can be grown in any type of soil under adverse climatic conditions.  Crop Improvement, nutritional requirement, water requirement, pest and disease management are the research priorities in Morinda citrifolia.

Indian Noni (Morinda citrifolia) is popularly known as Indian mulberry belongs to Rubiaceae family.  It is also called great Morinda, Cheese fruit, Mouse’s Pine Apple, Nono, Jumbie bread fruit, Hog apple. Noni was one of the most widely used medicinal plants in Polynesia prior to European contact, and continues popular among modern population. Noni is commonly known as canary wood in Australia, Kura in Fijii, mora de la India in spanish, nono in Tahiti and Nin in Marshall Island. Medicinal values of Noni were known to man many centuries ago. Uses of Noni were mentioned in Indian ethnopharmacology and ayurvedic medicines. Noni is grown as medicinal plant in most of  South Indian temples as sacred plant like basil (Tulasi). In India, it is called by several names in different  regional languages like nuna, manjanathi, manjanuna in Tamil,  molugu, mogal, bundamaddi in Telugu, kakaiplam, kattapitalavam, mannanatti in Malayalam, tagatemara, haladipavete in Kannada, Ach Al in Hindi, Paphan, Achchhulca in Sanskrit, Aseti, Nagkura, Mundari solidaru in Marathi.

Plants containing lignans have been utilised as folk medicines in traditional medicine of many different cultures for over 1000 years. Plants high in lignin, such as Kadsura coccinea (Schizandraceae), Fraxinus sp., and Olea europaea, have long been employed in Chinese, Japanese, and Eastern world folk medicine. Lignans are an important family of starting products for the development of new therapeutic agents based on structural modifications of such compounds due to their widespread use in traditional medicine. Podophyllum hexandrum, a plant with a high lignin concentration, has been employed throughout history and in modern times. It has been used extensively in ancient Ayurvedic medicine to treat a variety of disorders such as Condyloma acuminate, Taenia capitis, monocytoid leukemia, Hodgkin’s disease, non-Hodgkin’s Lymphoma, cancer of brain, lung, bladder and venereal warts.

The Himalayan locals have long used Indian Podophyllum, with an aqueous extract of the roots being a frequent cathartic. It has also been used as an ophthalmia treatment. Thomson tested the resin from the Indian plant in 1890 and found 56 percent podophyllotoxin concentration. Podwyssotzki discovered podophyllotoxin as the active principle of podophyllin in 1880 and obtained it in a pure state. Several lignans have been found in the rhizomes of Podophyllum hexandrum, which are dimerization products of phenylpropanoid pathway intermediates linked by the central carbons of their side chains.

ABSTRACT

Evolution theory postulates that the collision between the Indian and the Eurasian plates in early Eocene and Miocene epoch leads to formation of the Himalayas, and currently recognized across the globe as repository of life. Some species of this regions is categorized under endangered and endemic category. Rhododendrons placed under family Ericaceae is an important genus of flowering plants. It is one of the multiferous keystone member of plants, mostly endemic to Southeast Asia. Wide distribution of rhododendrons concentrated in Himalayas. The Eastern Himalaya, especially Arunachal Pradesh and Sikkim states of India is the main centre of species distribution and provide wide range of habitat for ecological studies. Several species exhibits many nutritional, medicinal and aromatic properties as evident from tribal knowledge and published research sciences. Different parts of plant such as flowers and leaves used in folklore herbal medicine and for preparation of local wines. Chemically, rhododendrons are repository of several bioactive molecules in the form of taraxerol, hyperoside, betulinic acid, quercitin, arbutin, rutin, coumaric acid, and several other fine molecules in minor quantities. In the present communication, Indian rhododendrons species diversity of different regions are revised and updated the current list for India. Besides, its value addition and future prospective is also presented for Indian economy. The checklist, chemistry, traditional knowledge, ecological requirement, keystone taxa, potential value added products for future, threat and future perspectives of rhododendrons for conservation is also discussed and presented in different subheads. First time checklist of all Indian rhododendrons are prepared and provided here in this communication.

Abstract

Medicinal plants are being widely used either as single drug or in combination in health care delivery system. Hemidesmus indicus is a rare and endangered plant of Deccan plateau of India and is a potential source of several active principles of therapeutic value. Its immense medicinal values can bring H. indicus as a royal source of herbal medicine in India. It contains various phytoconstituents belonging to the category glycosides, flavonoids, tannins, sterols and volatile oils. The main pharmacological activity of H. indicus include anti-inflammatory, anti-oxidants, anti cancerous activity, anti acne activity, anti venom activity, antinociceptive, renoprotective, heptoprotective activity, anti arthritis and anti leprotic activity. The root of Hemidesmus indicus was used mainly for the preparation of drink locally called as nannari or sugandhapala. The drink is medicinal, which cools the system, gives good appetite and acts as a blood purifier and roots are used for preparation of chutneys and pickles.

Abstract

India is known as hub of medicinal plants in recent years due to availability of variety of species in different regions which is used in treatment of frequently occurred local diseases. Family a pocynaceae is one of frequently used angiosperm members as medicine in different Indian system of medicine. At present, the genus Rauvolfia is represented by 77 species distributed across the globe, known to contains various unique active phytochemicals in the form of flavonoids, phytosterols, oleoresins, steroids, tannins and alkaloids. R. serpentina (also known as Indian snake root or sarpaghandha) and R. tetraphylla (also known as devil root or barachandrika) are two very important distinctive medicinal shrubs growing in India having wide application in traditional system of medicine for curing human disease and various herbal formulations available in market using these plants. In the present communication, we have discussed the biology, chemistry, pharmacology, cultivation practices and future perspectives of R. serpentina and R. tetraphylla for conservation and as a potential plants for future application in herbal medicine discovery.