eChapters

Epididymal Total Sperm Count

The epididymal sperm is obtained as described above, incubated at 35°C, which is the optimum temperature of rat epididymal sperm. The epididymal f luid is then diluted to a volume of 5 mL of pre-warmed (32°C) the spermatozoa are counted by hemocytometer using the Improved Neubaur (Deep 1/10mm. LABART, Germany) chamber as described by Pant and Srivastava (2003).

Total viable cell/ml = (Average number of sperm per chamber) x 103 x (Dilution Factor)

Liveability and abnormal sperm

Per cent live spermatozoa is estimated by differential staining technique using Eosin-Nigrosin stain (NE) (Campbell et al., 1953). These slides are also used for estimating the per cent abnormal sperm morphologically on the basis of observable abnormalities of head, neck, mid-piece and trail region of the spermatozoa.

The percent live spermatozoa are determined adopting the differential staining technique using Eosin-Nigrosin stain.

• A drop of epididymal semen is taken on a clean, grease free pre warmed glass slide.

• 4-5 drops of Eosin-Nigrosine stain (1% Eosin and 5% Nigrosine in 3% sodium citrate dehydrate solution) is placed near the semen drop.

• Epididymal semen and stain are mixed gently using a blunt fine glass rod.

• After 30 seconds to 1 minute a thin smear is made on a clean, grease free glass slide.

• The smear is examined under oil immersion objective.

India has a unique social and cultural setting with diverse people, culture and religions. It is one of the oldest and greatest civilizations of the world. Geographically India extends snow clad Himalayan mountains in the north to the tropical rain forests of the south. It is the 7th largest country in the world and well recognised in Asia by its mountains, seas, deserts, and vast stretch of fertile plain land, which give the country a distinct geographical identity.

India has a very rich, diverse religious and cultural heritage. The four main religions of India are - Hinduism, Islam, Christianity and Sikhism. Hinduism is the largest religion in India, with approximately 80% of the population is Hindu while Islam (Muslim) is the second-largest religion having 14% (approx.) share.

Religion in India is characterised by the diversity of religious beliefs and practices. In Indian history, religion has been an important part of the country's culture. Indian subcontinent is the birthplace of four of the world's major religions, namely, Buddhism, Hinduism, Jainism and Sikhism, which are collectively known as native Indian religions and represent 83% of the total population of India approximately.

Hinduism

Hinduism is the main Indian religion. It is considered as a religious and universal order, a way of life which followed by the people who believed in Hinduism. The word ‘Hindu’ refers group of particular people who believes and follow Hinduism, the oldest religion in the world. It has also been described as ‘Sanatana dharma’.

The Ramayana written by sage Valmiki is one of the great epics of the Sanskrit language. It is most religiously regarded and popular book in Hinduism. The book is believed to be complied between 300 B.C.E. – 200 AD. However, the original story could be as old 500 B.C.E. (Goldman, 1984). There are many other versions of the epic in various regional languages found in India and South Asia (Raghunathan, 1981; Arya,1998; Dutt, 2004; Mukherjee, 2015; Sharma and Arya, 2022).

As per literature references, the name ‘Ramanaya’ is composed of two words, ‘Rama’ and ‘Aya?a’. Rama, the name of the main character of the Epic, has two contextual meanings. In the Atharvaveda, it means 'dark’, dark-coloured, ‘black' and is related to the word ‘ratri’ (night) which means 'darkness of night'. The other meaning, which can be found in the Mahabharata, is 'pleasing’, pleasant, charming, lovely, beautiful. The word ‘Ayana’ means travel or journey. Thus, Ramaya?a means ‘Rama's Journey’ from his kingdom to various places, forests during his life time (Anonymous, 1960; Tulsidas, 1966; Murthy, 2003; Kishore, 2007; Rajagopalachari, 2013).

Ramayana contains over 24,000 couplet verses, in its full form, which are divided into following seven ‘Ka??as’ (booklets) - Balaka??a, Ayodhyaka??a, Ara?yaka??a, Ki?kindhaka??a, Sundaraka??a, Yuddhaka??a, Uttaraka??a and about 500 sargas (chapters). It is regarded as one of the longest epic poems written in history (Keshavadas, 1988; Debroy, 2017; Gupta, 2021).

morally right man in the world? Narada replied that such a man is Rama, son of King Dasharatha of Ayodhya. Valmiki wanted to know more about Rama and proceeded to take bath when he saw two birds in playful mood and was happy. Suddenly, a hunter hiding in the bush shot one of the two birds. Valmiki became painful and chanted some dialogues in curse which ultimately created a new form of sloka. This was the beginning of the main narrative (Goldman, 1984; Sachithanantham, 2004).

The exile of Rama and his journey to the forests (vanvasa) for 14 years was started from Ayodhya (Uttar Pradesh, India) and ended to Lanka (Sri Lanka), through different forests, territorial regions, lands, hills, lakes of old India. The whole journey to those places was candidly portrayed in Ramayana with vivid descriptions, from narrations of the night sky, the position of different stars to descriptions of the flora, fauna and landscape. In this chapter, a retrospection of the places, forests, Rama passed through during his entire journey has been documented. The present status of those forests and places has been considered to evaluate a comparison over the thousands of years and the changes taken place besides significance they hold till date (Anonymous, 2024; Iyer, 2014).

Journey to the Forest and Route Map

Ayodhya, the capital city, kingdom of Kosala, was situated on the banks of holy river Saryu and was the birthplace of Rama. To fulfill the wish of his father, king Dasharatha, Rama left Ayodhya for 14 years of exile to the forest, along with Sita and Lakshmana. Their journey started from Ayodhya and passed through different places / forests.

In this chapter, a comprehensive list of all plants and trees mentioned in epic Ramayana has been furnished with salient details in order to establish their botanical identity and references of Sholka where it had been mentioned. Some of the plants and trees had been mentioned in multiple sholokas but the plant has been taken into account as a single reference to avoid repetition and to get correct count of the trees and plants in whole of the Epic (Amirthalingam, 2013, Arun Kumar, 2019; Balapure et al., 1987; Bast, 2022; Ghimire et.al, 2021; Mao and Dash, 2010; Prasad and Mishra, 1976).

The plant species mentioned in Ramayana were of different types having various usages. Considering their morphological characteristics, usages and other importance, they have been categorized broadly into the following heads.

1. Economically important plants (Timber value, product yielding etc. having commercial importance).

2. Edible (major and minor fruits etc.).

3. Culturally important plants (Plants used for plantation for shade, greening etc.).

4. Non-Edible Plants (Ornamental importance and other usages)

5. Medicinal Plants (having therapeutic uses).

6. Sacred Plants (having religious importance).

7. Others (having multipurpose uses).

In previous Chapter, a list was furnished mentioning common names, botanical names, family and types of the all plants mentioned in the Ramayana. The purpose was to know the total number of plants and trees mentioned besides providing reference of the Shlokas as well. This information led to identification of the plants and trees but details about their morphological and floral characters were not mentioned. Therefore, in this Chapter details of the trees and other plants have been provided. This will help to know all about their botanical standing, conservation status, nativity, morphological and floral characters so that one can know the full details including present-day status, availability and importance (Cowen, 1950; Randhawa, 1983; Roy, 2015; 2024).

1. Botanical Name: Adenanthera pavonina L.

Synonym: Adenanthera gersenii Scheff.

Common Name: ‘Red Bead Tree’, ‘Curly Bean’, ‘Red Sandal Wood’

Family: Fabaceae

Nativity: India, Sri Lanka, Southeast Asia to Queensland

Conservation Status: Common (Not Threatened)

Type: Tree

Morphological and Floral Characters: Red Bead Tree is a timber tree found wild in India. Leaves compound, bipinnate, green when young, turning yellow on maturity. Flowers small, yellowish, grows in a dense, drooping almost cat tail like flower-heads. Fruits curved pods, hanging, green that turn brown, coil up and split open on ripening, showing small bright red seeds which are attractive, used as beads in jewellery.

Retrospection

India is a unique country in the world with many religions and cultural diversity. Indian culture has been continuing through the ages because of its adjustability with others and openness. Unity in diversity is one of the major characteristics of Indian culture. It is a diverse nation where people of many religions live together peacefully. Religion in India is characterized by the diversity of religious beliefs and practices. In Indian history, religion has played an important role in the cultural set up of the country.

Hinduism is the main Indian religion and considered as a universal order, a way of life which is followed by the people. The origin for Hinduism is very old having no exact date of its inception. This is a belief and practices followed by people through the ages and considered as a process of humanity. The earliest known sacred text of Hinduism is ‘Vedas’ which dates back to at least 3000 BCE. However, it is believed that these texts are of divine origin and timeless. Hinduism was more philosophically and theoretically described in Upanishads, written roughly between 800 and 400 BCE. The Mahabharata and Bhagavad-Gita are other best-known book and widely cited on Hinduism. The Ramayana is the other most important epic book on Hinduism which teaches about life, principles, duty, justice etc. through the life-story of Rama.

Dasharatha was a great Hindu king of the Kosala kingdom and Ayodhya was the capital from where He ruled. He was the son of King Aja of Kosala and Queen Indumati of Vidarbha. Dasharatha had three primary queens namely - Kausalya, Sumitra, and Kaikeyi. Unfortunately, the King had no sons to continue his dynasty after him. Despaired Dasharatha summoned Rishyashringa, a forest rishi for a ritual to fulfill his desire of have sons. By that way, the King had four sons. Queen Kaushalya gave birth Rama and similarly, Kaikeyi gave birth Bharata and Sumitra gave birth two sons, namely - Lakshmana and Shatrughna. Lakshmana was very much attached and devoted to Rama while Shatrughna was devoted to Bharata.

Plant hormone ethylene is involved in a number of physiological activities within the plant system, ranging from seed germination to fruit development. Ethylene acts as a key player in accelerating fruit ripening by regulating the activities of different enzymes, and is responsible for shortening the shelf life and associated post-harvest losses during the storage of fruit crops. However, all fruit crops have different ethylene production capacities and vary in their response to ethylene. Hence, effective management of post-harvest ethylene is imperative for addressing the global challenge of reducing fruit losses after harvesting. In recent periods, numerous technologies and compounds have been developed for the suppression of ethylene and for enhancing in-situ oxidation of ethylene or inhibiting its production at a receptor level. Among these emerging techniques, the use of sachets, fumigating agents, catalysis and composite films embedded with nanoparticles has been found to be trending, but the use of some of compounds has been limited due to health risks or consumer unacceptance. However, most of these technologies are very costly and are not affordable for marginal farmers and developing nations. In order to address these issues, long term breeding approaches for shelf-life improvement, targeting the tfs, ERFs have been involved. This manuscript fills the knowledge gap by encompassing all the recent progress made in enhancing the shelf life of fruits by managing ethylene during storage.

Fruit ripening is a phenomena during which horticulture produce undergoes a series of complex physical and biochemical changes initiated through a series of hormones (Ethylene, auxin, abscisic acid (ABA), gibberellins, jasmonic acid, etc.) to attain maximum physiological maturity and favourable organoleptic properties. These changes lead to alterations in colour, texture, aroma, flavour and nutrient composition. In a nut shell, ripening involves increase in pulp to peel ratio, solubilisation of pectic substances, degradation of complex carbohydrates, organic acids and chlorophyll, accumulation sugars, flavour volatiles, polyphenolic compounds and pigments (carotenoids, xanthophylls and anthocynanins). The extent of these changes guide harvesting, readiness for processing and consumption decisions, with physic-chemical alterations serving as indicators of optimum ripeness. Respiration rate, ethylene and ABA are key regulators of fruit ripening in climacteric and non-climacteric fruits, respectively. In climacteric fruits, ABA interacts with ethylene, influencing the synthesis of ethylene itself. Although there is ample documentation on the involvement of hormones in fruit development and ripening, our understanding of the functions of various hormones throughout growth, maturation, and specific ripening processes remains incomplete.

Calcium, anti-oxidants and certain enzymes are reported to play vital role in extending the shelf-life of harvested produce of many horticultural crops especially fruits. They help in maintenance of cell wall structure. Being an important constituent of cell wall, in the form of calcium pectate, it helps in maintenance of cell wall structure. Antioxidants are highly beneficial in extending the shelf-life of fruits besides maintaining the quality. Similarly, some enzymes play a key role in preserving the fruit quality and prolonging its market life. As some fruits are catergorized under ‘Low volume high value fruits’, it is essential to extend their shel-flife for reducing the post-harvest losses of fruits and also to reap better remuneration. Thus, it becomes indispensable to review the role of Ca, anti-oxidants and enzymes which are beneficial in keeping the harvested produce afresh for a relatively longer period.

Fruits, the vivid and delightful offerings of the nature, undergo remarkable transformations as they travel from the orchard to our dining tables. These changes indicate the significance of post-harvest physiology, which is the scientific study that reveals the secrets of how fruits behave after they are picked, including the mechanisms that affect fruit quality and how long it may be stored. The next section provides definitions of the various stages and key players involved in the processes of fruit maturity, ripening, and post harvest physiology. These topics will be further explored in the subsequent sub-sections of this chapter.

Fruit developmental physiology

The life cycle of horticultural commodities (fruits and vegetables) can be effectively categorized into three primary physiological stages after fruit set or initiation, namely growth, maturation, and senescence. Still, a lack of obvious differentiation exists among the several stages. Growth encompasses the process of cell division followed by cell enlargement, which determines the ultimate size of the product. Maturation typically begins prior to the cessation of growth and involves various activities across different commodities. The stages of growth and maturity are commonly denoted as the developmental phase. Senescence can be described as the phase in which anabolic biochemical processes transition into catabolic processes, resulting in the ageing and eventual demise of the tissue. Ripening, a term specifically used for fruit, is typically regarded as the initial phase of senescence that occurs during the later phases of development. Defining the transition from growth to senescence is rather straightforward.

Fruits and vegetables are perishables and the post-harvest losses from the point of production at the farm gate till it reaches the consumers is estimated as 30-35 per cent which constitute a major economic drain of more than 33 billion USD equivalent to Rs 2,40,000 crores annually. In order to reduce the losses at various stages, nanotechnologies have come as potential strategies to minimize the losses while improving the marketability and profitability of perishables. One of the plants derived natural compounds “hexanal” which is known to extend the shelf-life of fruits when it is used as a pre-harvest spray or post-harvest dip as a nano-emulsion. Since, it is a highly volatile compound, efforts were undertaken to entrap the compound in the polymer-based nano f ibres (as nano stickers) or as a cyclodextrin inclusion complex (as nano pellets) in order to preserve the perishables. Further, hexanal vapour was also evaluated to preserve fruits. In this book chapter, various nanotechnologies tested, evaluated and technologies developed over the years are elucidated. Overall, the literature review suggests that use of any of the nanotechnologies delivered as pre-harvest spray, post-harvest dip, nano-stickers, nano-pellets and vapour singly or in combination found to reduce the infestation of post harvest diseases, minimize the losses and extended shelf-life by 2-3 weeks that help the farmers to get lucrative prices due to the late arrival in the market besides significantly improve the productivity and profitability of orchards.

Introduction

The cold chain is a meticulously managed series of actions using specialized equipment to ensure products remain within a specific low-temperature range from production to consumption (ASHRAE, 2024; IIR, 2024). This unbroken sequence encompasses refrigerated production, storage, and distribution activities, safeguarding the safety and quality of perishable and thermo-sensitive goods like agricultural commodities (such as fresh produce, meat, dairy, seafood, chemicals, pharmaceuticals, flowers, wine, etc.), processed foods and pharmaceuticals (Aung and Chang, 2014; Awan, 2023). For climacteric fruits like bananas, custard apples, apples, pears, tomatoes, mangoes, and avocados, cold storage facilitates controlled ripening, extending the time between harvest and consumption. Cold chain management, integral to the overall supply chain, requires expertise in maintaining temperature, air quality, and adherence to regulatory standards. Any disruption in this process can lead to product spoilage and financial implications (FAO, 2019). The management of the cold chain system is crucial for preserving the freshness, taste, and nutritional value of fruits from harvest to consumption (Kader, 2003).

Introduction

Perishable nature of horticultural produce is a major factor contributing in high post-harvest losses. The metabolic processes such as respiration, transpiration, and ethylene production remain active after the harvesting of produce, which drive ripening and senescence Fig. 1. Thus, an effective post-harvest handling is crucial to delay these changes. The rate of these processes depends on the produce’s physiological characteristics, composition, and storage conditions like temperature and humidity. Low-temperature storage is the simplest and most efficient method to slow down deterioration Escobedo-Avellaneda and Welti-Chanes, 2016. Combining low temperatures with methods that limit oxygen exposure, which fuels many enzymatic reactions, further extends shelf life and maintains food quality. Reduced oxygen levels decrease respiration and the activity of enzymes like polyphenol oxidase and peroxidase.

Introduction

India is bestowed with varied agro-climatic conditions. Thus, horticultural crops have lot of potential in the country. India has witnessed voluminous increase in horticulture production over the last few years, which has undoubtedly doubled the income of farmers. As a result, the country has emerged as the 2nd largest producer of fruits (102.48 million tonnes) after China during 2020-21 (Anonymous, 2023). The abundance of fruits ranging from tropical delights like mango, banana, guava and papaya to temperate fruits such as apple, peach, plum, apricot and pear, have laid a robust foundation for the growth of the fruit processing industry. This chapter delves into the dynamics of fruit processed products in both local and international markets, exploring the challenges, opportunities and strategies for sustained success. Fruit processing plays a crucial role in adding value to horticultural produce, enhancing shelf-life and meeting the diverse demands of local and international markets. In India, a country blessed with abundant fruit varieties, the processed fruit industry has been witnessing significant growth, catering not only to the domestic market but also making strides in the global arena. The processing of these fruits into various products such as juices, pulps, concentrates, jams and dried fruits has emerged as a thriving industry. This chapter will provide insights into the current state of fruit processed products in India, their importance for both local consumption and export markets, challenges faced by the industry and potential avenues for the future growth.

Introduction

The horticulture sector is contributing over 30 per cent share in agricultural GDP of the country. Presently, India producing about 351.92 million tons of horticultural produce from 28.04 million hectare which has surpassed the food grain production for last five consecutive years (Anonymous, 2023). Horticulture sector has been identified by Government of India also identified horticulture as one of the vital sector for entrepreneurship development, employment generation, livelihood and nutritional security, doubling farmer’s income, achieving SDGs of the United Nations.

Fruits and vegetables have become part of a modern healthy lifestyle as they possess high amounts of minerals, especially electrolytes, dietary fiber, phytochemicals, especially antioxidants, and vitamins (Yahia et al., 2019; Krishnan Kesavan et al., 2023). In consumption, the qualities of products play a critical role in consumers’ choices, which are directly related to health problems (Tian, and Xu, 2022). Bioactive compounds are phytochemical substances present in small quantities in plant materials that can provide biological activity, being generally associated with health benefits, including hepatoprotective, anti-inflammatory, antioxidant, antithrombotic, anticarcinogenic, antidiabetic and prevention of cardiovascular and degenerative diseases (Campoli et al., 2018). Therefore, maintaining high-quality fruits and vegetables is very important for the fruit and vegetable industry. This requires some methods to evaluate the quality of fresh fruits and vegetables during their post-harvest manipulation and storage and before their industrial processing as well (Tian and Xu, 2022).

Waste is defined as anything that doesn’t add value to their operations. To the average person, waste is simply stuff that’s unwanted or useless. But from a scientific perspective, there’s really no such thing as waste. Nearly all the things we throw away can be transformed or processed in ways that make them useful again.

Waste comes in various forms, ranging from household trash to industrial byproducts. These include domestic waste, factory waste, e-waste, construction waste, agricultural waste, and more. We can categorize waste into different types:

Solid waste: This includes organic materials like vegetable and kitchen waste, as well as general household waste.

E-waste: Discarded electronic devices such as computers, TVs, and music systems fall into this category.

Liquid waste: This comprises water used in various industries, as well as waste from tanneries, distilleries, and thermal power plants.

Plastic waste: Items like plastic bags, bottles, and containers make up this category.

Introduction

A wide range of agricultural pesticides is currently being used in India. In the financial year of 2015, the size of the crop protection market in India was of about $4.5 billion (FICCI, 2016). A large number of these pesticides are also being used in considerable quantity on horticultural crops, including fruit crops. In general, judicious application of a pesticide following Good Agricultural Practices (GAP) does not leave its residues in crop produces above unacceptable limit. But the GAP recommendations are not available for all crop-pesticide combinations. The usage pattern of pesticides also sometimes gets modified at farm level due to sudden climate changes, and under such situations, the indiscriminate use of pesticides to combat disease and insect complex might result in the contamination of pesticides in fruits.

Although after independence, the use of hybrid and composite seeds of various crops, especially of rice and wheat resulted in an increase in the Indian agricultural output leading to “Green Revolution”. This Green Revolution came to fore during the late “Nineteen Hundred and Sixty”. In fact, during the era of “Green Revolution”, introduction of high yielding varieties of rice and wheat crops were grown which responded to more use of fertilizers, pesticides as well as water.

There is no doubt that “Green Revolution” made the country-India, self- sufficient in food grains production, particularly of rice and wheat. As a matter of fact, the “Green Revolution” created a buffer stock at times exceeding to 60 million tonnes, which made the nation or the country very proud (Mahajan and Gupta, 2009). However, continuous use of chemical fertilizers as well as inorganic pesticides and totally neglect of organic manures and botanical pesticides prepared from various plant species like Neem (Azadirachta indica) and Drek (Melia azedarach) for controlling of insects came totally to stand still. So very soon, the deleterious or ill effects of the “Green Revolution” the so called the chemical-based farming or “Industrial agriculture,” came to fore, which briefly stand discussed below

History

The history of organic farming is largely based upon the history of organic movement which came to fore as a reaction against the large-scale use of chemical fertilizers and pesticides in agriculture. This movement can be traced back to England during 1920, when individuals started speaking against a variety of agricultural “innovation”.

One of the earlier pioneers of Organic Farming, Sir Albert Howard, is often referred to as the father of the modern “Organic Farming”. He began to work as a British botanist and served as an agriculture advisor in India, where he observed traditional Indian farming practices and came to respect them as superior to his conventional agriculture science. He documented and developed these organic farming practices/methods. His writings and notably the 1940 book, An Agricultural Testament, have influenced many scientists and farmers of the day.

In Germany Dr. Rudolf Steiner developed biodynamic agriculture which was probably the first comprehensive farming system. In America, the agronomist F.H. King had toured China, Korea and Japan in early 1990s, closely studied the fertilization, tillage and general farming practices being followed there. He published his findings in his book Farmers of Forty Centuries (1911). King probably did not impart any view himself as part of the movement, whether Organic Farming movement or by some other name. However, in the later years, some scientists within the organic farming movement thought, about what King had encountered in Asia as kindred approach.